IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ cooper-2005-1 A screen for proteins that interact with PAX6: C-terminal mutations disrupt interaction with HOMER3, DNCL1 and TRIM11. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Cooper ST., Hanson IM. Apoptosis - Interactions involving proteins with a function related to apoptosis simon.t.cooper@ed.ac.uk mouse brain cDNA library. BMC Genet. 2005 Only protein-protein interactions imex curation cooper-2005-2 A screen for proteins that interact with PAX6: C-terminal mutations disrupt interaction with HOMER3, DNCL1 and TRIM11. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Cooper ST., Hanson IM. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 3, table 1 simon.t.cooper@ed.ac.uk mouse brain cDNA library. BMC Genet. 2005 Only protein-protein interactions imex curation dyl1_mouse Dynein light chain 1, cytoplasmic Dynll1 Dlc1 Dnclc1 Dncl1 Dynein light chain LC8-type 1 8 kDa dynein light chain Protein inhibitor of neuronal nitric oxide synthase protein protein mouse Mus musculus Mouse MCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG pax6_human Paired box protein Pax-6 Oculorhombin Aniridia type II protein PAX6 AN2 protein protein human Homo sapiens Human MQNSHSGVNQLGGVFVNGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRYYETGSIRPRAIGGSKPRVATPEVVSKIAQYKRECPSIFAWEIRDRLLSEGVCTNDNIPSVSSINRVLRNLASEKQQMGADGMYDKLRMLNGQTGSWGTRPGWYPGTSVPGQPTQDGCQQQEGGGENTNSISSNGEDSDEAQMRLQLKRKLQRNRTSFTQEQIEALEKEFERTHYPDVFARERLAAKIDLPEARIQVWFSNRRAKWRREEKLRNQRRQASNTPSHIPISSSFSTSVYQPIPQPTTPVSSFTSGSMLGRTDTALTNTYSALPPMPSFTMANNLPMQPPVPSQTSSYSCMLPTSPSVNGRSYDTYTPPHMQTHMNSQPMGTSGTTSTGLISPGVSVPVQVPGSEPDMSQYWPRLQ home3_human Homer protein homolog 3 HOMER3 protein protein human Homo sapiens Human MSTAREQPIFSTRAHVFQIDPATKRNWIPAGKHALTVSYFYDATRNVYRIISIGGAKAIINSTVTPNMTFTKTSQKFGQWADSRANTVYGLGFASEQHLTQFAEKFQEVKEAARLAREKSQDGGELTSPALGLASHQVPPSPLVSANGPGEEKLFRSQSADAPGPTERERLKKMLSEGSVGEVQWEAEFFALQDSNNKLAGALREANAAAAQWRQQLEAQRAEAERLRQRVAELEAQAASEVTPTGEKEGLGQGQSLEQLEALVQTKDQEIQTLKSQTGGPREALEAAEREETQQKVQDLETRNAELEHQLRAMERSLEEARAERERARAEVGRAAQLLDVSLFELSELREGLARLAEAAP tri11_human E3 ubiquitin-protein ligase TRIM11 RING finger protein 92 Protein BIA1 TRIM11 RNF92 RING-type E3 ubiquitin transferase TRIM11 Tripartite motif-containing protein 11 protein protein human Homo sapiens Human MAAPDLSTNLQEEATCAICLDYFTDPVMTDCGHNFCRECIRRCWGQPEGPYACPECRELSPQRNLRPNRPLAKMAEMARRLHPPSPVPQGVCPAHREPLAAFCGDELRLLCAACERSGEHWAHRVRPLQDAAEDLKAKLEKSLEHLRKQMQDALLFQAQADETCVLWQKMVESQRQNVLGEFERLRRLLAEEEQQLLQRLEEEELEVLPRLREGAAHLGQQSAHLAELIAELEGRCQLPALGLLQDIKDALRRVQDVKLQPPEVVPMELRTVCRVPGLVETLRRFRGDVTLDPDTANPELILSEDRRSVQRGDLRQALPDSPERFDPGPCVLGQERFTSGRHYWEVEVGDRTSWALGVCRENVNRKEKGELSAGNGFWILVFLGSYYNSSERALAPLRDPPRRVGIFLDYEAGHLSFYSATDGSLLFIFPEIPFSGTLRPLFSPLSSSPTPMTICRPKGGSGDTLAPQ pax6-dncl1-2 Y2H: bait = PAX6 PST domain, prey = Dncl1 329064 N/A 329066 unspecified role unspecified role prey prey N/A 329067 unspecified role unspecified role bait bait pst domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false pax6-homer3-1 Y2H: bait = PAX6 c-terminus peptide, prey =HOMER3 329064 N/A 329068 unspecified role unspecified role prey prey N/A 329067 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false pax6-dncl1-1 Y2H: bait = PAX6 c-terminus peptide, prey =Dncl1 329064 N/A 329066 unspecified role unspecified role prey prey N/A 329067 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false pax6-homer3-2 Y2H: bait = PAX6 PST domain, prey = HOMER3 329064 N/A 329067 unspecified role unspecified role bait bait pst domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 329068 unspecified role unspecified role prey prey physical association physical association false false false pax6-trim11-1 Y2H: bait = PAX6 PST domain, prey = Trim11 329064 N/A 329067 unspecified role unspecified role bait bait pst domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 329069 unspecified role unspecified role prey prey physical association physical association false false false pax6-dncl1-3 Y2H: bait = PAX6 PST domain, prey = Dncl1 329065 N/A 329067 unspecified role unspecified role bait bait c-terminus required but not sufficient required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pst domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 329066 unspecified role unspecified role prey prey physical association physical association false false false The following mutations in PAX6 disrupt the interaction with Dcln1: 1629insT mutation changing the stop codon by a Leucine at the c-terminus of PAX6 (X423L) generating a 35 amino acids extension, 1615del10 mutation, a PAX6 frameshift mutation causing replacement of the last 5 amino-acids by a 103 amino acid extension. Mutations found in aniridia (OMIM:106210) disrupts the interaction. pax6-homer3-3 Y2H: bait = PAX6 PST domain, prey = HOMER3 329065 N/A 329067 unspecified role unspecified role bait bait pst domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false c-terminus required but not sufficient required to bind necessary binding region required to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 329068 unspecified role unspecified role prey prey c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The following mutations in PAX6 disrupt the interaction with Dcln1: 1629insT mutation changing the stop codon by a Leucine at the c-terminus of PAX6 (X423L) generating a 35 amino acids extension, 1615del10 mutation, a PAX6 frameshift mutation causing replacement of the last 5 amino-acids by a 103 amino acid extension. Mutations found in aniridia (OMIM:106210) disrupts the interaction. pax6-trim11-2 Y2H: bait = PAX6 PST domain, prey = Trim11 329065 N/A 329069 unspecified role unspecified role prey prey N/A 329067 unspecified role unspecified role bait bait truncated pst domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false