IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ reid-2004-1 Crystallographic analysis of CaaX prenyltransferases complexed with substrates defines rules of protein substrate selectivity. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Reid TS., Terry KL., Casey PJ., Beese LS. Apoptosis - Interactions involving proteins with a function related to apoptosis Farnesyl transferase were cocrystalized with a peptide substrate thanks to the use of farnesyl diphosphate (FPP) analog : FPT-II J. Mol. Biol. (0022-2836) 2004 reid-2004-2 Crystallographic analysis of CaaX prenyltransferases complexed with substrates defines rules of protein substrate selectivity. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Reid TS., Terry KL., Casey PJ., Beese LS. Apoptosis - Interactions involving proteins with a function related to apoptosis Farnesyl transferase were cocrystalized with a peptide substrate thanks to the use of farnesyl diphosphate (FPP) analog : FPT-II J. Mol. Biol. (0022-2836) 2004 rap2a_human Ras-related protein Rap-2a RbBP-30 RAP2A protein protein human Homo sapiens Human MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYEKVPVILVGNKVDLESEREVSSSEGRALAEEWGCPFMETSAKSKTMVDELFAEIVRQMNYAAQPDKDDPCCSACNIQ fntb_human Protein farnesyltransferase subunit beta CAAX farnesyltransferase subunit beta Ras proteins prenyltransferase subunit beta FNTB protein protein human Homo sapiens Human MASPSSFTYYCPPSSSPVWSEPLYSLRPEHARERLQDDSVETVTSIEQAKVEEKIQEVFSSYKFNHLVPRLVLQREKHFHYLKRGLRQLTDAYECLDASRPWLCYWILHSLELLDEPIPQIVATDVCQFLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDIINREKLLQYLYSLKQPDGSFLMHVGGEVDVRSAYCAASVASLTNIITPDLFEGTAEWIARCQNWEGGIGGVPGMEAHGGYTFCGLAALVILKRERSLNLKSLLQWVTSRQMRFEGGFQGRCNKLVDGCYSFWQAGLLPLLHRALHAQGDPALSMSHWMFHQQALQEYILMCCQCPAGGLLDKPGKSRDFYHTCYCLSGLSIAQHFGSGAMLHDVVLGVPENALQPTHPVYNIGPDKVIQATTYFLQKPVPGFEELKDETSAEPATD fnta_human Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha CAAX farnesyltransferase subunit alpha Ras proteins prenyltransferase subunit alpha FTase-alpha Type I protein geranyl-geranyltransferase subunit alpha FNTA protein protein human Homo sapiens Human MAATEGVGEAAQGGEPGQPAQPPPQPHPPPPQQQHKEEMAAEAGEAVASPMDDGFVSLDSPSYVLYRDRAEWADIDPVPQNDGPNPVVQIIYSDKFRDVYDYFRAVLQRDERSERAFKLTRDAIELNAANYTVWHFRRVLLKSLQKDLHEEMNYITAIIEEQPKNYQVWHHRRVLVEWLRDPSQELEFIADILNQDAKNYHAWQHRQWVIQEFKLWDNELQYVDQLLKEDVRNNSVWNQRYFVISNTTGYNDRAVLEREVQYTLEMIKLVPHNESAWNYLKGILQDRGLSKYPNLLNQLLDLQPSHSSPYLIAFLVDIYEDMLENQCDNKEDILNKALELCEILAKEKDTIRKEYWRYIGRSLQSKHSTENDSPTNVQQ rras2_human Ras-related protein R-Ras2 Ras-like protein TC21 Teratocarcinoma oncogene RRAS2 TC21 protein protein human Homo sapiens Human MAAAGWRDGSGQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVIDDRAARLDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF fnta_rat Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha CAAX farnesyltransferase subunit alpha Ras proteins prenyltransferase subunit alpha FTase-alpha Type I protein geranyl-geranyltransferase subunit alpha Fnta protein protein rat Rattus norvegicus (Rat) Rat MAATEGVGESAPGGEPGQPEQPPPPPPPPPAQQPQEEEMAAEAGEAAASPMDDGFLSLDSPTYVLYRDRAEWADIDPVPQNDGPSPVVQIIYSEKFRDVYDYFRAVLQRDERSERAFKLTRDAIELNAANYTVWHFRRVLLRSLQKDLQEEMNYIIAIIEEQPKNYQVWHHRRVLVEWLKDPSQELEFIADILNQDAKNYHAWQHRQWVIQEFRLWDNELQYVDQLLKEDVRNNSVWNQRHFVISNTTGYSDRAVLEREVQYTLEMIKLVPHNESAWNYLKGILQDRGLSRYPNLLNQLLDLQPSHSSPYLIAFLVDIYEDMLENQCDNKEDILNKALELCEILAKEKDTIRKEYWRYIGRSLQSKHSRESDIPASV fntb_rat Protein farnesyltransferase subunit beta CAAX farnesyltransferase subunit beta Ras proteins prenyltransferase subunit beta Fntb protein protein rat Rattus norvegicus (Rat) Rat MASSSSFTYYCPPSSSPVWSEPLYSLRPEHARERLQDDSVETVTSIEQAKVEEKIQEVFSSYKFNHLVPRLVLQREKHFHYLKRGLRQLTDAYECLDASRPWLCYWILHSLELLDEPIPQIVATDVCQFLELCQSPDGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYNVINREKLLQYLYSLKQPDGSFLMHVGGEVDVRSAYCAASVASLTNIITPDLFEGTAEWIARCQNWEGGIGGVPGMEAHGGYTFCGLAALVILKKERSLNLKSLLQWVTSRQMRFEGGFQGRCNKLVDGCYSFWQAGLLPLLHRALHAQGDPALSMSHWMFHQQALQEYILMCCQCPAGGLLDKPGKSRDFYHTCYCLSGLSIAQHFGSGAMLHDVVMGVPENVLQPTHPVYNIGPDKVIQATTHFLQKPVPGFEECEDAVTSDPATD rash_human GTPase HRas HRAS HRAS1 Transforming protein p21 p21ras H-Ras-1 c-H-ras Ha-Ras protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS p01116-2 GTPase KRas KRAS KRAS2 RASK2 K-Ras 2 Ki-Ras c-K-ras c-Ki-ras K-Ras4B protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM Variant in position: 152:V->G (in NS3). Variant in position: 153:D->V (in CFC2 and NS3, exhibits only minor alterations in its in vitro biochemical behavior compared to wild-type protein). Variant in position: 156:F->I (in NS3/CFC2). Variant in position: 156:F->L (found in a patient with Costello syndrome, exhibits an increase in intrinsic and guanine nucleotide exchange factor catalyzed nucleotide exchange in combination with an impaired GTPase-activating protein-stimulated GTP hydrolysis but functional in interaction with effectors). tc21 TC21 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide human Homo sapiens Human KKSKTKCVIF pgtb1_rat Geranylgeranyl transferase type-1 subunit beta Geranylgeranyl transferase type I subunit beta Type I protein geranyl-geranyltransferase subunit beta Pggt1b protein protein rat Rattus norvegicus (Rat) Rat MAATEDDRLAGSGEGERLDFLRDRHVRFFQRCLQVLPERYSSLETSRLTIAFFALSGLDMLDSLDVVNKDDIIEWIYSLQVLPTEDRSNLDRCGFRGSSYLGIPFNPSKNPGTAHPYDSGHIAMTYTGLSCLIILGDDLSRVDKEACLAGLRALQLEDGSFCAVPEGSENDMRFVYCASCICYMLNNWSGMDMKKAISYIRRSMSYDNGLAQGAGLESHGGSTFCGIASLCLMGKLEEVFSEKELNRIKRWCIMRQQNGYHGRPNKPVDTCYSFWVGATLKLLKIFQYTNFEKNRNYILSTQDRLVGGFAKWPDSHPDALHAYFGICGLSLMEESGICKVHPALNVSTRTSERLRDLHQSWKTKDSKQCSDNVHISS rhob_human Rho-related GTP-binding protein RhoB Rho cDNA clone 6 RHOB ARH6 ARHB protein protein human Homo sapiens Human MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCPNVPIILVANKKDLRSDEHVRTELARMKQEPVRTDDGRAMAVRIQAYDYLECSAKTKEGVREVFETATRAALQKRYGSQNGCINCCKVL gbg2_human Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 G gamma-I GNG2 protein protein human Homo sapiens Human MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL p60953-2 Cell division control protein 42 homolog CDC42 G25K GTP-binding protein Placental protein protein human Homo sapiens Human MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL pfta-rap2-1 X-Ray: Human Farnesyl transferase complexed with Rap2A c-terminus peptide (containing CAAX motif) 188893 N/A 188895 unspecified role unspecified role neutral component neutral component caax motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 188896 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) N/A 188897 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false 1.80A working 17.9%, free 19.9% the Rap2A peptide used does not match 100% the entry: DDPTASACNIQ pfta-rras2-1 X-Ray: Rat Farnesyl transferase complexed with TC21 c-terminus peptide (containing CAAX motif) 188893 N/A 188898 unspecified role unspecified role neutral component neutral component caax motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false kkksktk tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro N/A 188899 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 188900 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false 2.30A working 18.0%, free 20.7% the TC21 peptide used does not match 100% the entry, it has been mutated to make it more soluble: KKSKTKCVIF pfta-rash-1 X-Ray: Rat Farnesyl transferase complexed with H-Ras c-terminus peptide (containing CAAX motif) 188893 N/A 188900 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 188901 unspecified role unspecified role neutral component neutral component caax motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P01112:p.Lys185Gly mutation mutation certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 188899 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false 2.25A working 19.3%, free 21.8% the H-Ras peptide used does not match 100% the entry: GCVLS pfta-kras-1 X-Ray: Rat Farnesyl transferase complexed with K-Ras4B (ki-Ras isoform 2) c-terminus peptide (containing CAAX motif) 188893 N/A 188899 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 188902 unspecified role unspecified role neutral component neutral component in vitro In vitro N/A 188900 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false 2.70A working 19.4%, free 21.1% pfta-rras2-2 X-Ray: Rat Farnesyl transferase complexed with TC21 c-terminus peptide (containing CAAX motif) 188893 N/A 188903 unspecified role unspecified role neutral component neutral component in vitro In vitro N/A 188899 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 188900 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false 2.85A working 18.9%, free 21.0% the TC21 peptide used does not match 100% the entry, it has been mutated to make it more soluble: KKSKTKCVIF pgt1-rras2-1 X-Ray: Rat Geranylgeranyl transferase complexed with TC21 c-terminus peptide (containing CAAX motif) 188894 N/A 188898 unspecified role unspecified role neutral component neutral component caax motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false kkksktk tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro N/A 188899 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 188904 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false 2.85A working 18.9%, free 21.0% the TC21 peptide used does not match 100% the entry, it has been mutated to make it more soluble: KKKSKTKCVIF pgt1-kras-1 X-Ray: Geranylgeranyl transferase complexed with K-Ras4B (ki-Ras isoform 2) c-terminus peptide (containing CAAX motif) 188894 N/A 188902 unspecified role unspecified role neutral component neutral component in vitro In vitro N/A 188899 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false The participant EBI-602447(Q04631) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later. 2.70A working 19.4%, free 21.1% pgt1-rhob-1 X-Ray: Geranylgeranyl transferase complexed with RhoB c-terminus peptide (containing CAAX motif) 188894 N/A 188899 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 188905 unspecified role unspecified role neutral component neutral component caax motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false The participant EBI-602447(Q04631) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later. 2.70A working 19.3%, free 21.2% pgt1-gng2-1 X-Ray: Geranylgeranyl transferase complexed with gamma subunit of heterotrimeric Gprotein c-terminus peptide (containing CAAX motif) 188894 N/A 188906 unspecified role unspecified role neutral component neutral component caax motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 188899 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false The participant EBI-602447(Q04631) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later. 2.70A working 19.3%, free 21.2% pgt1-cdc42-1 X-Ray: Geranylgeranyl transferase complexed with cdc42 c-terminus peptide (containing CAAX motif) 188894 N/A 188907 unspecified role unspecified role neutral component neutral component caax motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 188899 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false The participant EBI-602447(Q04631) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later. 2.90A working 19.9%, free 21.8%