UniProt UniProt http://www.uniprot.org cohen-2004a-1 Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu Science (0036-8075) 2004 Only protein-protein interactions imex curation cohen-2004a-2 Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. in vitro In vitro deacetylase assay deacetylase assay western blot western blot Immuno blot Immuno blot Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu Science (0036-8075) 2004 Only protein-protein interactions imex curation cohen-2004a-3 Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu Science (0036-8075) 2004 Only protein-protein interactions imex curation cohen-2004a-4 Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. in vitro In vitro deacetylase assay deacetylase assay predetermined predetermined participant predetermined Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu Science (0036-8075) 2004 Only protein-protein interactions imex curation xrcc6_human X-ray repair cross-complementing protein 6 XRCC6 G22P1 ATP-dependent DNA helicase II 70 kDa subunit Lupus Ku autoantigen protein p70 70 kDa subunit of Ku antigen Thyroid-lupus autoantigen CTC box-binding factor 75 kDa subunit DNA repair protein XRCC6 X-ray repair complementing defective repair in Chinese hamster cells 6 ATP-dependent DNA helicase 2 subunit 1 5'-deoxyribose-5-phosphate lyase Ku70 protein protein human Homo sapiens Human MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD sir1_human NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 SIRT1 SIR2L1 SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein human Homo sapiens Human MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS bax_human Apoptosis regulator BAX BAX BCL2L4 Bcl-2-like protein 4 protein protein human Homo sapiens Human MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD sirt1-xrcc6-1 1241658 n/a 1241662 unspecified role unspecified role prey prey n/a 1241663 unspecified role unspecified role bait bait physical association physical association false false false 3A xrcc6-sirt1-1 1241658 n/a 1241662 unspecified role unspecified role bait bait n/a 1241663 unspecified role unspecified role prey prey physical association physical association false false false 3A sirt1-xrcc6-2 1241659 n/a 1241662 enzyme target enzyme target substrate substrate neutral component neutral component lys-539 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1241663 enzyme enzyme neutral component neutral component deacetylation deacetylation reaction deacetylation false false false 3c sirt1-xrcc6-3 1241659 n/a 1241663 enzyme enzyme neutral component neutral component n/a 1241662 enzyme target enzyme target substrate substrate neutral component neutral component lys452 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro deacetylation deacetylation reaction deacetylation false false false 3c bax-xrcc6 1241660 n/a 1241662 unspecified role unspecified role prey prey n/a 1241664 unspecified role unspecified role bait bait physical association physical association false false false 4C SIRT1 (UniProt Ac Q96EB6). Interaction is decreased by siRNA reduced SIRT1 or catalytically inactive SIRT1 construct. xrcc6-bax 1241660 n/a 1241664 unspecified role unspecified role prey prey n/a 1241662 unspecified role unspecified role bait bait physical association physical association false false false 4C SIRT1 (UniProt Ac Q96EB6). Interaction is decreased by siRNA reduced SIRT1 or catalytically inactive SIRT1 construct. sirt1-tp53-9 1241661 n/a 1241663 enzyme enzyme neutral component neutral component n/a 1241665 enzyme target enzyme target substrate substrate neutral component neutral component lys-320 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false deacetylation deacetylation reaction deacetylation false false false 3e sirt1-xrcc6-4 1241661 n/a 1241663 enzyme enzyme neutral component neutral component n/a 1241662 enzyme target enzyme target substrate substrate neutral component neutral component lys539 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false lys-542 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false deacetylation deacetylation reaction deacetylation false false false 3e