IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ahn-2004-3 Calmodulin binding to the Fas death domain. Regulation by Fas activation. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ahn EY., Lim ST., Cook WJ., McDonald JM. Apoptosis - Interactions involving proteins with a function related to apoptosis A CaM pull down assay was used (Jurkat cell lystaes were incubated with CaM-Sepharose to isolate binding proteins) J. Biol. Chem. (0021-9258) 2004 ahn-2004-1 Calmodulin binding to the Fas death domain. Regulation by Fas activation. human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot Ahn EY., Lim ST., Cook WJ., McDonald JM. Apoptosis - Interactions involving proteins with a function related to apoptosis A CaM pull down assay was used (Jurkat cell lystaes were incubated with CaM-Sepharose to isolate binding proteins) J. Biol. Chem. (0021-9258) 2004 ahn-2004-2 Calmodulin binding to the Fas death domain. Regulation by Fas activation. human-jurkat Homo sapiens jurkat Human T cell leukemia coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Ahn EY., Lim ST., Cook WJ., McDonald JM. Apoptosis - Interactions involving proteins with a function related to apoptosis A CaM pull down assay was used (Jurkat cell lystaes were incubated with CaM-Sepharose to isolate binding proteins) J. Biol. Chem. (0021-9258) 2004 calm_human Calmodulin CALM1 CALM CAM CAM1 CALM2 CAMB CALM3 CAM3 CAMC CAMIII CAM2 CALML2 protein protein human Homo sapiens Human MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK tnr6_human Tumor necrosis factor receptor superfamily member 6 FASLG receptor Apoptosis-mediating surface antigen FAS Apo-1 antigen FAS APT1 TNFRSF6 FAS1 protein protein human Homo sapiens Human MLGIWTLLPLVLTSVARLSSKSVNAQVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPETVAINLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQLLRNWHQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLV cam-cd95-1 The a region of the cytoplasmic domain of the CD95(Fas) protein (residues 230-270) interacts with the calmodulin protein 173772 N/A 173775 unspecified role unspecified role bait bait in vitro In vitro N/A 173776 unspecified role unspecified role prey prey region Fas death domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Binding of CD95(Fas)(residues 230 - 270) to CaM-Sepharose required the presence of calcium. cam-cd95pm Point mutations within the cytoplasmic domain of the CD95 protein reduce binding affinity for Calmodulin 173772 N/A 173776 unspecified role unspecified role prey prey region cytoplasmic domian binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P25445:p.Thr241Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 173776 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region cytoplasmic domian binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P25445:p.Phe248Ala Phenylalanine to alanine point mutation reduces binding to calmodulin mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 173776 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region cytoplasmic domian binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P25445:p.Gly247Phe Glycine to phenylalanine point mutation reduces binding to calmodulin mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 173776 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region cytoplasmic domian binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 173775 unspecified role unspecified role bait bait region Calmodulin sepharose tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro association association false false false cam-cd95 The calmodulin protein interacts with the CD95(Fas) protein 173773 N/A 173775 unspecified role unspecified role bait bait in vitro In vitro N/A 173776 unspecified role unspecified role prey prey physical association physical association false false false Binding of CD95(Fas)to CaM-Sepharose required the presence of calcium. cam-cd95-2 The CD95 (Fas) protein binds to calmodulin in vivo 173774 N/A 173776 unspecified role unspecified role prey prey N/A 173775 unspecified role unspecified role bait bait physical association physical association false false false