IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mihara-2003-2 p53 has a direct apoptogenic role at the mitochondria. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation mihara-2003-1 p53 has a direct apoptogenic role at the mitochondria. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation mihara-2003-6 p53 has a direct apoptogenic role at the mitochondria. human-ml-1 Human myeloblastic leukemia ML-1 cell line ml-1 Myeloblastic leukemia ML-1 cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis The co-inmunoprecipitation was performed using purified mitochondria from this cell line. Cells were pre-treated with camptothecin (induces intrinsic apoptosis). umoll@notes.cc.sunysb.edu Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation mihara-2003-5 p53 has a direct apoptogenic role at the mitochondria. human-rko Homo sapiens colorectal carcinoma cell line rko Human colorectal carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis The co-inmunoprecipitation was performed using purified mitochondria from this cell line. Cells were pre-treated with camptothecin (induces intrinsic apoptosis). umoll@notes.cc.sunysb.edu Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation mihara-2003-3 p53 has a direct apoptogenic role at the mitochondria. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation mihara-2003-4 p53 has a direct apoptogenic role at the mitochondria. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation q07817-1 Bcl-2-like protein 1 BCLX Apoptosis regulator Bcl-X BCL2L1 BCL2L Bcl-xL protein protein human Homo sapiens Human MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD bcl2_human Apoptosis regulator Bcl-2 BCL2 protein protein human Homo sapiens Human MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK bcl2l1-tp53-1 1268348 n/a 1268354 unspecified role unspecified role bait bait n/a 1268355 unspecified role unspecified role prey prey mitochondria-targeting tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 3b tp53-bcl2l1-1 1268348 n/a 1268355 unspecified role unspecified role bait bait mitochondria-targeting tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1268354 unspecified role unspecified role prey prey physical association physical association false false false 3b and 7d bcl2l1-tp53-2 1268348 n/a 1268355 unspecified role unspecified role prey prey n/a 1268354 unspecified role unspecified role bait bait physical association physical association false false false 3b bcl2-tp53 1268348 n/a 1268355 unspecified role unspecified role prey prey mitochondria-targeting tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1268356 unspecified role unspecified role bait bait physical association physical association false false false 3d tp53-bcl2l1-8 1268348 n/a 1268355 unspecified role unspecified role bait bait n/a 1268354 unspecified role unspecified role prey prey physical association physical association false false false 7d tp53-bcl2l1 1268349 n/a 1268355 unspecified role unspecified role bait bait mitochondria-targeting tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1268354 unspecified role unspecified role prey prey physical association physical association false false false 3a bcl2l1-tp53 1268349 n/a 1268354 unspecified role unspecified role bait bait n/a 1268355 unspecified role unspecified role prey prey mitochondria-targeting tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 3a tp53-bcl2l1-12 1268349 n/a 1268354 unspecified role unspecified role prey prey n/a 1268355 unspecified role unspecified role bait bait P04637:p.Arg175His mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P04637:p.Arg280Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P04637:p.Arg248Thr mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P04637:p.Leu194Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 7e bcl2l1-tp53-5 1268350 n/a 1268354 unspecified role unspecified role bait bait n/a 1268355 unspecified role unspecified role prey prey physical association physical association false false false 4a right bcl2-tp53-1 1268350 n/a 1268355 unspecified role unspecified role prey prey n/a 1268356 unspecified role unspecified role bait bait physical association physical association false false false 4b right tp53-bcl2-4 1268350 n/a 1268355 unspecified role unspecified role bait bait n/a 1268356 unspecified role unspecified role prey prey physical association physical association false false false 4b left bcl2l1-tp53-4 1268351 n/a 1268354 unspecified role unspecified role bait bait n/a 1268355 unspecified role unspecified role prey prey physical association physical association false false false 4a left tp53-bcl2l1-3 1268351 n/a 1268354 unspecified role unspecified role prey prey n/a 1268355 unspecified role unspecified role bait bait physical association physical association false false false 4a left, 7c bcl2l1-tp53-3 1268352 n/a 1268354 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1268355 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false 3e left tp53-bcl2l1-2 1268353 n/a 1268355 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1268354 unspecified role unspecified role prey prey purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3e right