IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nagao-2003-1 MAGE-A4 interacts with the liver oncoprotein gankyrin and suppresses its tumorigenic activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nagao T., Higashitsuji H., Nonoguchi K., Sakurai T., Dawson S., Mayer RJ., Itoh K., Fujita J. Apoptosis - Interactions involving proteins with a function related to apoptosis jfujita@virus.kyoto-u.ac.jp Human placenta or a U-2 OS cDNA library J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer nagao-2003-3 MAGE-A4 interacts with the liver oncoprotein gankyrin and suppresses its tumorigenic activity. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Nagao T., Higashitsuji H., Nonoguchi K., Sakurai T., Dawson S., Mayer RJ., Itoh K., Fujita J. Apoptosis - Interactions involving proteins with a function related to apoptosis jfujita@virus.kyoto-u.ac.jp J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer Human placenta or a U-2 OS cDNA library nagao-2003-5 MAGE-A4 interacts with the liver oncoprotein gankyrin and suppresses its tumorigenic activity. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Nagao T., Higashitsuji H., Nonoguchi K., Sakurai T., Dawson S., Mayer RJ., Itoh K., Fujita J. Apoptosis - Interactions involving proteins with a function related to apoptosis jfujita@virus.kyoto-u.ac.jp J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer Human placenta or a U-2 OS cDNA library nagao-2003-2 MAGE-A4 interacts with the liver oncoprotein gankyrin and suppresses its tumorigenic activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nagao T., Higashitsuji H., Nonoguchi K., Sakurai T., Dawson S., Mayer RJ., Itoh K., Fujita J. Apoptosis - Interactions involving proteins with a function related to apoptosis jfujita@virus.kyoto-u.ac.jp J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer Human placenta or a U-2 OS cDNA library nagao-2003-4 MAGE-A4 interacts with the liver oncoprotein gankyrin and suppresses its tumorigenic activity. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nagao T., Higashitsuji H., Nonoguchi K., Sakurai T., Dawson S., Mayer RJ., Itoh K., Fujita J. Apoptosis - Interactions involving proteins with a function related to apoptosis jfujita@virus.kyoto-u.ac.jp J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer Human placenta or a U-2 OS cDNA library maga4_human Melanoma-associated antigen 4 MAGE-4 antigen MAGE-X2 antigen MAGE-41 antigen Cancer/testis antigen 1.4 MAGEA4 MAGE4 protein protein human Homo sapiens Human MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPASNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV psd10_human 26S proteasome non-ATPase regulatory subunit 10 26S proteasome regulatory subunit p28 Gankyrin PSMD10 p28(GANK) protein protein human Homo sapiens Human MEGCVSNLMVCNLAYSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVAKGGLGLILKRMVEG prs6b_human 26S proteasome regulatory subunit 6B Proteasome 26S subunit ATPase 4 MB67-interacting protein PSMC4 TBP7 MIP224 Tat-binding protein 7 26S proteasome AAA-ATPase subunit RPT3 protein protein human Homo sapiens Human MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK gankyrin-mage_a4 Yeast 2 hybrid analysis reveals interaction between Gankyrin protein and the MAGE-A4 protein 661943 N/A 661948 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 661949 unspecified role unspecified role bait bait physical association physical association false false false gankyrin-psmc4 Yeast 2 hybrid analysis reveals interaction between Gankyrin protein and the 26S protease regulatory subunit 6B protein 661943 N/A 661949 unspecified role unspecified role bait bait N/A 661950 unspecified role unspecified role prey prey physical association physical association false false false The authors state that the C-terminal region of the 26S protease regulatory subunit 6B protein binds to the bait protein , but do not give the coordinates of this region. gankyrin-mage_a4-2 Coimmunoprecipitation analysis reveals interaction between Gankyrin protein and the MAGE-A4 protein 661944 N/A 661948 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 661949 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1B gankyrin-mage_a4-3 Coimmunoprecipitationanalysis reveals interaction between Gankyrin protein and the MAGE-A4 protein 661944 N/A 661948 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 661949 cofactor cofactor coenzyme bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1B gankyrin-rb Coimmunoprecipitationanalysis reveals interaction between Gankyrin protein and the Rb protein 661945 N/A 661951 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 661949 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3A gankyrin-psmc4-1 Coimmunoprecipitationanalysis reveals interaction between Gankyrin protein and the 26S protease regulatory subunit 6B protein 661945 N/A 661949 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 661950 unspecified role unspecified role prey prey region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3B gankyrin-mage_a4-1 Yeast 2 hybrid analysis reveals interaction between Gankyrin protein and the MAGE-A4 protein 661946 N/A 661948 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 661949 unspecified role unspecified role bait bait physical association physical association false false false 1A 4B gankyrin-mage_a4-4 Coimmunoprecipitation analysis reveals interaction between Gankyrin protein and the MAGE-A4 protein 661947 N/A 661948 unspecified role unspecified role bait bait N/A 661949 unspecified role unspecified role prey prey physical association physical association false false false 2C