IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ cavdar_koc-2001-1 The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present. bovin-liver Bos taurus liver liver liver density sedimentation cosedimentation through density gradient density sedimentation de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Cavdar Koc E., Burkhart W., Blackburn K., Moseley A., Spremulli LL. Apoptosis - Interactions involving proteins with a function related to apoptosis Bovine mitochondria and 28 S subunits were isolated from liver tissue and the 28 S subunits were collected by centrifugation at 48,000 rpm for 6 h in a Beckman Type-50 rotor. For analysis of individual proteins the complex was separated on two-dimensional gels followed by isolation of distinct protein spots which were trypsin digested or directly digest by trypsin of fractions from the centrifuged lysate. Protein identification was determined by analysis ogf the digested proteins using LC-MS-MS. linda_spremulli@unc.edu J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation rt18c_bovin 28S ribosomal protein S18c, mitochondrial MRPS18C 28S ribosomal protein S18-1, mitochondrial protein protein bovin Bos taurus (Bovine) Bovine MAAVLGLCGGLGKRKFTRFPTAFVCLTNSGTRAVLWRSCSQCKQVTSSEDLPIPMENPYKEPLKKCILCEKRVDYKNVQLLSQFISPFTGCIYGRHITGLCGKKQREITKAIKRAQILGFMPVTYKDPAYLKDPKVCNIRYRE rt29_bovin 28S ribosomal protein S29, mitochondrial DAP3 MRPS29 Death-associated protein 3 protein protein bovin Bos taurus (Bovine) Bovine MLKGMTRLVSRVHKLDPGRFSHLGTQAPQCPVAHLDNQVPTERTRAISRTLENDPAKHGEQHVGQHYNISIQELKTVFPHGLPPRFVMQVKTFNEACLMVRKPALELLHYLKNTNFAHPAVRYVLYGEKGTGKTLSLCHIIHFCAKQDWLILHIPDAHLWVKNCRDLLQSTYNKQRFDQPLEASIWLKNFKTANERFLSQIKVQDKYVWNKRESTEKGSPLAEVVEQGIMRVRNATDAVGIVLKELKRQSSLGVFRLLVAVDGVNALWGRTTLKREDKSPITPEELALIYNLRKMVKNDWQGGAIVLTVSQTGSLFKPRKAYLPQELLGKEGFDTLDPFIPILVSNYNPKEFEGCIQYYLENNWLQHEKAHTEEGKKELLFLSNRNPGLLERLCAYL rt16_bovin 28S ribosomal protein S16, mitochondrial MRPS16 RPMS16 protein protein bovin Bos taurus (Bovine) Bovine MVQLTTVLCKAYRGGHLTIRLALGGCTNRPFYRIVAAHNKCPRDGRFVEQLGSYDPMPNSHGEKLVALNLDRIRHWIGCGAHLSKPVEKLLGLSGFFPLHPMVITNAERLRRKRAQEVLLAAQKTDTEATETKEN rm42_bovin 39S ribosomal protein L42, mitochondrial MRPL42 MRPS32 28S ribosomal protein S32, mitochondrial protein protein bovin Bos taurus (Bovine) Bovine MALAAVKWAISSRTMLKHLFPVENGALYCVGHKSTYSSLPDDYNCKVELALTSDARTIVCYHPSVDIPYEHTKPIPRPDPVHNNEETHDLVLKTRLEEKSEHLEQGPMIEQLSKMFFTTKHRWYPRGQYHRRRRKLNPPKDR rt06_bovin 28S ribosomal protein S6, mitochondrial MRPS6 RPMS6 protein protein bovin Bos taurus (Bovine) Bovine MPRYELALILKAMQRPETAAALKRTLEALMDRGAVVRSLENLGERTLPYKMSAHSQRHTRGGYFLVDFYAPTTTVASIMEHLSRDIDVIRPNVVKHPLTQEVKECEGIVPVPLEEKLYSTKKRK rt30_bovin 28S ribosomal protein S30, mitochondrial MRPS30 protein protein bovin Bos taurus (Bovine) Bovine MAATRCWRFVLRSPGLSLHTAAEATVTAPEVTGSDVKAAPVARYPPIVASLTADSKAARQRRVERWQATVHAAKSVDEKLRILTKMQFMKYVVYPQTFALNADRWYQSFTKTVFLSGLPPPQAQPDREPAQVVDLAALRAAVCDCLLQEHFFLRRKKRAPIYQERYAVASPFLDQLVPSLTGLLSAYNPVLAAAALDCNRPVHFYWLRGEEIIPGGHRKGRVDAVRYQINDKPHNQIRISRQLPEFVPLDYSVPVEVPVKNCKPDKLPLFKRQYENAIFIGTKTADPLCYGHTQFHLLPDKLKRERLLKQNCADQIEVIFRANAIASLFAWTGAQAMYQGFWSEADVTRPFVSQGVITDGKYFSFFCYQLNTLALTAQADQNNPRKNICWGTQSMPLYETIEDNDVKGFNDDVLLQLVHFLLNRPEEDKAQLLVN rt33_bovin 28S ribosomal protein S33, mitochondrial MRPS33 protein protein bovin Bos taurus (Bovine) Bovine MSSLSEYALRMSRLSARLFSEVARPTDSKSMKVVKLFSEQPLAKRKETYDWYPNHNTYFALMGILRSVGLYRDEHQDFKDEQLRLKKLRGKVKPRKGEGKRAAKKK rt02_bovin 28S ribosomal protein S2, mitochondrial MRPS2 protein protein bovin Bos taurus (Bovine) Bovine MATGAVLPRLLGAGVRAAPRGRAAQRGRTLGSAAAAAAREPERDSDRSARILSEPLKHSDFFNVKELFSVRSLFNARVHLGHKAGCRHRFMEPYIFGSRLGQDIIDLEQTATHLQLALNFTAHVAFRGGIILFVSRARQFSHLIESTARSCGEYAHTRYFKGGLLTNAPLLLGARVRLPDLIIFLHTLNNVFEPHVAVRDAAKMSIPTVGVVDTNCNPCLITYPVPGNDDSPPAVQLFCQLFQTAVTRAKEKRRQLEALYRLQGAPGPHPANPAAPGAPSPGAQAQLGMGHSP rt36_bovin 28S ribosomal protein S36, mitochondrial MRPS36 Alpha-ketoglutarate dehydrogenase component 4 KGD4 protein protein bovin Bos taurus (Bovine) Bovine MMGSKMASASRVVQVVKPHTPLIRFPDRRDNPKPNVSEVLRSAGLPSHTSSISQHSKGSKSPDWLMHQGPPDTAEMIKTLPQKYRRKLVSQEEIEFIQRGGPE rt34_bovin 28S ribosomal protein S34, mitochondrial MRPS34 protein protein bovin Bos taurus (Bovine) Bovine MARRKVRPRLIAELARRVRALREQRERPRDSVRYALDYETLIRPHSGRKLPLRAWVDVRRESRLLQLLGRLPFFGLGRLVTRKSWLWQHDEPCYWRLTRVRPDYTAQNLDHGKAWGILTFKGKTESEAREIEQVMHHDWRLVPKHEEAAFTSFTPAPEETPRPVPYPPLLRAMILAERQKNGDPSTEEPMLSLERIRTDPWDYPENQEAKKKTKGTAV rt18a_bovin 28S ribosomal protein S18a, mitochondrial MRPS18A 28S ribosomal protein S18-3, mitochondrial protein protein bovin Bos taurus (Bovine) Bovine MAALNVLVSGCGRFLRGLLTGPTVTSWARPPARGFREVVEIQEGKTTIIEGRITGTPKESPNPPNPSGQCPICRWNLKHKYSYEDVLLLSQFIRPHGGMLPRSITGLCQEEHRKIEECVKMAHRAGLLPNHRPKLPEGFFPKTRPRLNRYLTRWSPRSVKPIYNKGHRWNKVRMAVGSPLLKDNVSYTGRPLVLYH rt18b_bovin 28S ribosomal protein S18b, mitochondrial MRPS18B 28S ribosomal protein S18-2, mitochondrial protein protein bovin Bos taurus (Bovine) Bovine MAASVLNVLLRRLPYFSPFRGAYGVQVPLQTLCTKAPPEDDSLPPIPVSPYEDEPWKYLDSEEYHNRNGSRPVWADYRRNHKGGIPPQRTRKMCIRGNKVAGNPCPICRDQKLHVDFRNVKLLKQFVCAHTGIIFHAPYTGVCMKQHKKLTQAIQKARDHGLLSYHIPQVEPRDLDFSTSHGAVSATPPAPTLVSGDPWYPWYSWKQPPERELSRLRRLYQGHLREESGPPPESMPKVPLTAPNEATSTEQAGPQSAL rt15_bovin 28S ribosomal protein S15, mitochondrial MRPS15 RPMS15 protein protein bovin Bos taurus (Bovine) Bovine MLRAAWRALSSIRTQAVTQPPVLGLPGGGCAKLLSVQRDLPSSLILQAARGYATQKPVQQSQEDDPPPSMLLKDYQNVPGIEKVDDVVKRLISLEMANKKEMLKIKKEQLMSKVVENPKDTSSLEAQIVALTVKIRSYEEHMQKHQKDKAHKRYLLMSIDKRQKMLKNLRKTNYPVFEKTCKELGIEYTFPPPYHRKIHRRLATKKALCIRVFQEVQKLKKQKRALKTAAAAAQKQGQRNPESPSEARSEAIKETQ rt28_bovin 28S ribosomal protein S28, mitochondrial MRPS28 protein protein bovin Bos taurus (Bovine) Bovine MAALCRTRAVTAESHFLRVFLFSRSCRGAGTESGSGSESSNSTEPRPRPGGFASALERHSELQRKAELARTRGSPKNVESFASMLRHSPLTQMGPAKDKIVIGRIFHIVENDLYIDFGGKFHCVCKRPEVDGEKYQKGTRVRLRLLDLELTSRFLGATTDTTILEAEAVLLGLQESKDSKSKEERRENK rt17_bovin 28S ribosomal protein S17, mitochondrial MRPS17 RPMS17 protein protein bovin Bos taurus (Bovine) Bovine MSVVRSSVHAKWIVGKVIGTAMQKTAKVRVTRLVLDPYLLKYFNKRKTYFAHDALQQCTVGDIVLLKALPVPRTKHVKHELAEIVFKVGQVVDPVTGKRCAGTTYLESPVDLETTPLAKNLEELSLSTTQ rt21_bovin 28S ribosomal protein S21, mitochondrial MRPS21 RPMS21 protein protein bovin Bos taurus (Bovine) Bovine MAKHLKFIARTVMVQEGNVEGAYRTLNRILTMDGLIEDIKRRRYYEKPCRRRQRESYETCRRIYNMEMARKINFLMRKNRADPWQGC rt11_bovin 28S ribosomal protein S11, mitochondrial MRPS11 protein protein bovin Bos taurus (Bovine) Bovine MQVLRNSGSWLLRSWAWPPTTRVVAGVPAPTIHMSAQQMQDAAAKEEVEKAETPAPAPSRSSFSIYPPIPGQESSLRWAGKKFEEIPIAHIKASYNNTQIHVVSAAHQPLARASCGTEGFRNAKKGTGIAAQTAGIAAAAKATGKGVTHVRVVVKGLGPGRLSAIKGLTMGGLEVISITDNTPIPHNGCRPRKARRL rt31_bovin 28S ribosomal protein S31, mitochondrial MRPS31 protein protein bovin Bos taurus (Bovine) Bovine MFPRVSAVLPFRPLSRLPLCSAGPEASAATVVPLASPHGTVRTKCNIQRYFGTNSVIYSKKDDKSVPACEISKETENQGSTKENKKKDLVNIIKGMKVELSTVNVQTTKPPNRGQLKSLEAAIRRLQKSPEDAPQKSKSLSPELVAAATAVADSLPFDKQTTKSELLRQLRQHEEDSKAQKDGEKPKISFSNIISDMKVARSSTARASTRPVHQIQFDEGADDFVDREETADLRKRFRKNIFKGKRLNIFELKPVTEEAPETEAAPSLWDVEFAKQLAAVTEQPFQNGFEEMIQWTKEGKLWEFPINNEAGFDDDGSEFHEHIFLDKYLEGFPKQGPIRHFMELVTCGLSKNPYLSVKQKVEHIEWFRNYFNEKQDILKESGINFS rt1-rt02 Protein components of the bovine small mitoribosomal subunit 545066 N/A 545067 unspecified role unspecified role neutral component neutral component N/A 545068 unspecified role unspecified role neutral component neutral component N/A 545069 unspecified role unspecified role neutral component neutral component N/A 545070 unspecified role unspecified role neutral component neutral component N/A 545071 unspecified role unspecified role neutral component neutral component N/A 545072 unspecified role unspecified role neutral component neutral component N/A 545073 unspecified role unspecified role neutral component neutral component N/A 545074 unspecified role unspecified role neutral component neutral component N/A 545075 unspecified role unspecified role neutral component neutral component N/A 545076 unspecified role unspecified role neutral component neutral component N/A 545077 unspecified role unspecified role neutral component neutral component N/A 545078 unspecified role unspecified role neutral component neutral component N/A 545079 unspecified role unspecified role neutral component neutral component N/A 545080 unspecified role unspecified role neutral component neutral component N/A 545081 unspecified role unspecified role neutral component neutral component N/A 545082 unspecified role unspecified role neutral component neutral component N/A 545083 unspecified role unspecified role neutral component neutral component N/A 545084 unspecified role unspecified role neutral component neutral component physical association physical association false false false Table 1