IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ghosh-2019-3 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in The FEBS journal 2019 Only protein-protein interactions imex curation imex curation ghosh-2019-4 in vitro In vitro atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in The FEBS journal 2019 Only protein-protein interactions imex curation imex curation ghosh-2019-1 in vitro In vitro aggregation aggregation assay aggregation predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease Figure 8; Experiments are stated to be using TMR-Ab42 in the image text, and monitored by ThT fluorescence. The image itself does not mention TMR and as TMR tagged Ab42 is monitored by a decrease in TMR fluorescence it has been assumed that Ab42 is NOT tagged. Amyloid formation was monitored by Thioflavin T (ThT) fluorescence which exhibits a fluorescence emission maximum at ~488nm exclusively when bound to amyloid. Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in The FEBS journal 2019 Only protein-protein interactions imex curation imex curation ghosh-2019-2 in vitro In vitro aggregation aggregation assay aggregation predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease Figure 8; Experiments are stated to be using TMR-Ab42 in the image text, and monitored by ThT fluorescence. The image itself does not mention TMR and as TMR tagged Ab42 is monitored by a decrease in TMR fluorescence it has been assumed that Ab42 is NOT tagged. Aggregation is monitored by quenching of TMR fluorescence. Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in The FEBS journal 2019 Only protein-protein interactions imex curation imex curation ghosh-2019-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in The FEBS journal 2019 Only protein-protein interactions imex curation imex curation ghosh-2019-6 in vitro In vitro fcs fluorescence correlation spectroscopy FCS FCS fcs predetermined predetermined participant predetermined Ghosh S., Sil TB., Dolai S., Garai K. Alzheimers - Interactions investigated in the context of Alzheimers disease Abeta-42 is of Human origin based on the sequence given on aapptec web-site and APOE is of Human origin based on mutation mapping. Source of recombinant APOE traced to pmid:25207746 kanchan@tifrh.res.in The FEBS journal 2019 Only protein-protein interactions imex curation imex curation p02649-pro_0000001987 Apolipoprotein E APOE protein protein human Homo sapiens Human KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH 19 317 p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 apoe-app-4 3648788 N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false HDF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept edans_label fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept edans_label fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 5A, 8C, Supp. fig 10. The APOE*4 variant :P02649 VAR_000652. Native tryptophan residues in apoE are the FRET donor and EDANS tagged to ab-42 is the acceptor. The slow time-dependent increase of FRET is consistent with interactions of apoE with species of Ab42 such as the ‘oligomers’ that form in a time-dependent manner. apoe-app-5 3648788 N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Ala62Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Ser139Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Trp210Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Trp276Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) 3648808 3648809 3648810 3648812 3648813 3648814 3648815 3648816 3648817 3648818 direct interaction direct interaction false false false 5B, Supp. fig 6 The APOE*4 variant :P02649 VAR_000652. Figure 5B shows that the kinetics of FRET efficiency between apoE and Ab are similar for all the five cysteine mutants. Similar results are obtained with EDANS-labelled lipidated apoE (Suppl Fig. 6) apoe-app-6 3648788 N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) 3648825 3648826 direct interaction direct interaction false false false 6A,7A, 8D, Supp. fig 7,8. The APOE*4 variant :P02649 VAR_000652. It has been assumed the APOE mutation "W247C" is a typo and was meant to be A247C. Figure 7:FRET is measured as a decrease in donor fluorescence at t=14hrs, rather than FRET efficiency. apoe-app-7 3648788 N/A 3648794 competitor competitor competitor unspecified role unspecified role purified purified P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) 3648836 3648837 direct interaction direct interaction false false false 6A-2, 7A-2, 8D, Supp. Fig. 7 The APOE*4 variant :P02649 VAR_000652. It has been assumed the APOE mutation "W247C" is a typo and was meant to be A247C. The reduction of the FRET signal is very small in the presence of the (unlabelled) APOE4 fragments but high in the presence of the full-length apoE4. Figure 7: FRET is measured as a decrease in donor fluorescence at t=14hrs, rather than FRET efficiency. This effect is reversed in the presence of a competitor, full length APOE, but less so in the presence of NTF and CTF mixed together. apoe-app-9 3648788 N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 3648845 3648846 3648854 3648855 direct interaction direct interaction false false false 6B,7B The APOE*4 variant :P02649 VAR_000652. It has been assumed the APOE mutation "W247C" is a typo and was meant to be A247C. Figure 7:FRET is measured as a decrease in donor fluorescence rather than FRET efficiency. apoe-app-10 3648788 N/A 3648794 competitor competitor competitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 3648860 3648861 3648869 3648870 direct interaction direct interaction false false false 6B-2,7B-2 The APOE*4 variant :P02649 VAR_000652. It has been assumed the APOE mutation "W247C" is a typo and was meant to be A247C. FRET is reduced strongly in the presence of unlabelled full-length apoE4 but affected modestly in the presence of unlabelled NTF or CTF or NTF + CTF. Figure 7:FRET is measured as a decrease in donor fluorescence rather than FRET efficiency. apoe-app-11 3648788 N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 3648879 3648880 direct interaction direct interaction false false false 6C The APOE*4 variant :P02649 VAR_000652. apoe-app-12 3648788 N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648794 competitor competitor competitor unspecified role unspecified role purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 3648888 3648889 direct interaction direct interaction false false false 6C-2 The APOE*4 variant :P02649 VAR_000652. apoe-app-13 3648788 N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648794 stimulator stimulator unspecified role unspecified role purified purified CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 3648900 3648901 direct interaction direct interaction false false false 6C-3 The APOE*4 variant :P02649 VAR_000652. The presence of an excess amount of CTF appears to enhance the FRET between the EDANS-NTF and TMR-Ab42. apoe-app-14 3648788 N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Ala102Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648794 competitor competitor competitor unspecified role unspecified role purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 3648913 3648914 direct interaction direct interaction false false false 6C-4 The APOE*4 variant :P02649 VAR_000652. The NTF fragment is a less effective competitor than the full length protein. apoe-app-15 3648788 N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) 3648921 3648922 direct interaction direct interaction false false false 6D The APOE*4 variant :P02649 VAR_000652. apoe-app-16 3648788 N/A 3648794 competitor competitor competitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) 3648931 3648932 direct interaction direct interaction false false false 6D-2 The APOE*4 variant :P02649 VAR_000652. apoe-app-17 3648788 N/A 3648794 competitor competitor competitor unspecified role unspecified role purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) 3648943 3648944 direct interaction direct interaction false false false 6D-3 The APOE*4 variant :P02649 VAR_000652. The CTF and NTF fragments are less effective competitors than full length APOE. apoe-app-18 3648788 N/A 3648794 competitor competitor competitor unspecified role unspecified role purified purified CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648794 unspecified role unspecified role fluorescence donor fluorescence donor purified purified P02649-PRO_0000001987:p.Ala247Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false edans_label fluorophore fluorophore certain certain sequence position certain certain certain sequence position certain false CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) 3648954 3648955 direct interaction direct interaction false false false 6D-4 The APOE*4 variant :P02649 VAR_000652. The CTF and NTF fragments are less effective competitors than full length APOE. apoe-app-8 3648789 N/A 3648794 inhibitor inhibitor unspecified role unspecified role purified purified NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false HDF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Supp. fig. 1 The inhibitory effect on aggregation of ab-42 is the highest with the full-length apoE4 followed by the NTF, CTF and HDF. Fragments of apoE (Apoe4) and full length Apoe3 and Apoe4, both in the lipid-free and in the lipidated forms, interact with Ab-42 to delay the kinetics of fibrillization. app-app-127 3648789 N/A 3648795 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Supp. fig. 1. app-app-128 3648789 N/A 3648795 unspecified role unspecified role neutral component neutral component edans_label fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role neutral component neutral component edans_label fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Supp. fig. 5. app-app-125 3648790 N/A 3648795 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1A-E, 8 A/B, Supp. fig. 2. app-app-121 3648790 N/A 3648795 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648794 inhibitor inhibitor unspecified role unspecified role purified purified NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false HDF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1A-E, 2, 8 A/B, supp. fig. 2 The inhibitory effect on aggregation of ab-42 is the highest with the full-length apoE4 followed by the NTF, CTF and HDF. Fragments of apoE (Apoe4) and full length Apoe3 and Apoe4, both in the lipid-free and in the lipidated forms, interact with Ab-42 to delay the kinetics of fibrillization. apoe-app-3 3648791 N/A 3648794 inhibitor inhibitor unspecified role unspecified role purified purified NTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false CTF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false HDF_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P02649-PRO_0000001987:p.Cys112Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3, Supp. fig.3 The inhibitory effect on aggregation of ab-42 is the highest with the full-length apoE4 followed by the NTF, CTF and HDF. Both apoE3 and apoE4 delay the kinetics of aggregation of Ab42 both in lipid-free as well as in lipidated forms. app-app-126 3648791 N/A 3648795 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3, Supp. fig. 3 apoe-app-19 3648792 N/A 3648794 unspecified role unspecified role neutral component neutral component purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Supp. fig.9D apoe-app-20 3648793 N/A 3648794 unspecified role unspecified role neutral component neutral component purified purified P02649-PRO_0000001987:p.Cys112Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3648795 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3648795 unspecified role unspecified role neutral component neutral component n-terminus tetramethyl rhodamine label tetramethyl rhodamine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Supp. fig.9E-I apoE-Ab form complexes that are highly heterogeneous and oligomeric in nature.