IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ elkabets-2015-1 human-cal33 CAL 33-Human tongue squamous cell carcinoma cell line Human cal33 CAL 33-Human tongue squamous cell carcinoma cell line DSMZ no.: ACC 447 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. CAL33-R cells-a BYL719 (PIK3CA inhibitor) resistant cell line This publication refers to various phosphorylated tyrosine residues whose presence is detected by site specific antibodies. The location of these phosphorylated tyrosine residues as has been mapped to the canonical UniProt entry for EGFR (P00533) by reference to peptide sequences listed in Chattopadhyay et, 1999, pmid: 10473558. scaltrim@mskcc.org,baselgaj@mskcc.org Cancer cell 2015 Only protein-protein interactions imex curation imex curation Cancer - Interactions investigated in the context of cancer elkabets-2015-2 human-cal33 CAL 33-Human tongue squamous cell carcinoma cell line Human cal33 CAL 33-Human tongue squamous cell carcinoma cell line DSMZ no.: ACC 447 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. CAL33-R cells-a BYL719 (PIK3CA inhibitor) resistant cell line scaltrim@mskcc.org,baselgaj@mskcc.org Cancer cell 2015 Only protein-protein interactions imex curation imex curation Cancer - Interactions investigated in the context of cancer elkabets-2015-3 human-kyse-180 KYSE-180 Esophageal squamous cell Carcinoma Cell Line Human kyse-180 KYSE-180 Esophageal squamous cell Carcinoma Cell Line DSMZ no.: ACC 379 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. KYSE-180 R cells-a BYL719 (PIK3CA inhibitor) resistant cell line scaltrim@mskcc.org,baselgaj@mskcc.org Cancer cell 2015 Only protein-protein interactions imex curation imex curation Cancer - Interactions investigated in the context of cancer elkabets-2015-4 human-kyse-180 KYSE-180 Esophageal squamous cell Carcinoma Cell Line Human kyse-180 KYSE-180 Esophageal squamous cell Carcinoma Cell Line DSMZ no.: ACC 379 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Elkabets M., Pazarentzos E., Juric D., Sheng Q., Pelossof RA., Brook S., Benzaken AO., Rodon J., Morse N., Yan JJ., Liu M., Das R., Chen Y., Tam A., Wang H., Liang J., Gurski JM., Kerr DA., Rosell R., Teixidó C., Huang A., Ghossein RA., Rosen N., Bivona TG., Scaltriti M., Baselga J. Affinomics - Interactions curated for the Affinomics consortium. KYSE-180 R cells-a BYL719 (PIK3CA inhibitor) resistant cell line This publication refers to various phosphorylated tyrosine residues whose presence is detected by site specific antibodies. The location of these phosphorylated tyrosine residues as has been mapped to the canonical UniProt entry for EGFR (P00533) by reference to peptide sequences listed in Chattopadhyay et, 1999, pmid: 10473558. scaltrim@mskcc.org,baselgaj@mskcc.org Cancer cell 2015 Only protein-protein interactions imex curation imex curation Cancer - Interactions investigated in the context of cancer ufo_human Tyrosine-protein kinase receptor UFO UFO AXL oncogene AXL protein protein human Homo sapiens Human MAWRCPRMGRVPLAWCLALCGWACMAPRGTQAEESPFVGNPGNITGARGLTGTLRCQLQVQGEPPEVHWLRDGQILELADSTQTQVPLGEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTPFNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGPQRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATITVLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPLTHCTLQAVLSDDGMGIQAGEPDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTHWLPVETPEGVPLGPPENISATRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPSTPAFSWPWWYVLLGAVVAAACVLILALFLVHRRKKETRYGEVFEPTVERGELVVRYRVRKSYSRRTTEATLNSLGISEELKEKLRDVMVDRHKVALGKTLGEGEFGAVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSERESFPAPVVILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRQGNRLKQPADCLDGLYALMSRCWELNPQDRPSFTELREDLENTLKALPPAQEPDEILYVNMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAPGQEDGA egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA egfr-axl-2 1808116 N/A 1808120 unspecified role unspecified role prey prey N/A 1808121 unspecified role unspecified role bait bait N/A optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false N/A optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4H, 6C, supp 6B The cells in the resistant model (CAL33R) show increased EGFR and AXL interaction. R428 - a small molecule ATP competitor that inhibits the AXL by in this instance preventing the specific interaction between (phosphoryated) residue 1197 (1173) of EGFR with AXL. egfr-axl-1 1808117 N/A 1808120 unspecified role unspecified role neutral component neutral component N/A 1808121 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 4I, Supp 6C The number of AXL/EGFR complexes is significantly higher in resistant cell lines compared to the parental counterpart. R428 - a small molecule ATP competitor that inhibits the AXL by in this instance preventing the specific interaction between (phosphoryated) residue 1197 (1173) of EGFR with AXL. axl-egfr-1 1808118 N/A 1808120 unspecified role unspecified role neutral component neutral component N/A 1808121 unspecified role unspecified role neutral component neutral component tyr-1197 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false proximity proximity close-contact colocalization neighbourhood interaction false false false 4I, supp 6C The number of AXL/EGFR complexes is significantly higher in resistant cell lines compared to the parental counterpart. R428 - a small molecule ATP competitor that inhibits the AXL by in this instance preventing the specific interaction between (phosphoryated) residue 1197 (1173) of EGFR with AXL. egfr-axl-3 1808119 N/A 1808120 unspecified role unspecified role prey prey N/A 1808121 unspecified role unspecified role bait bait N/A optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false N/A optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4H, Supp 6B The cells in the resistant model (CAL33R) show increased EGFR and AXL interaction. R428 - a small molecule ATP competitor that inhibits the AXL by in this instance preventing the specific interaction between (phosphoryated) residue 1197 (1173) of EGFR with AXL.