IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ni-2014-1 Interaction of integrin β4 with S1P receptors in S1P- and HGF-induced endothelial barrier enhancement. human-endothelium Homo sapiens endothelail cells/endothelium endothelium Endothelial cells [endothelium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ni X., Epshtein Y., Chen W., Zhou T., Xie L., Garcia J.G., Jacobson J.R. Affinomics - Interactions curated for the Affinomics consortium. Lung endothelial cells jrjacob@uic.edu J. Cell. Biochem. (0730-2312) 2014 Only protein-protein interactions imex curation ni-2014-2 Interaction of integrin β4 with S1P receptors in S1P- and HGF-induced endothelial barrier enhancement. human-endothelium Homo sapiens endothelail cells/endothelium endothelium Endothelial cells [endothelium] pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Ni X., Epshtein Y., Chen W., Zhou T., Xie L., Garcia J.G., Jacobson J.R. Affinomics - Interactions curated for the Affinomics consortium. Lung endothelial cells jrjacob@uic.edu J. Cell. Biochem. (0730-2312) 2014 Only protein-protein interactions imex curation itb4_human Integrin beta-4 GP150 ITGB4 protein protein human Homo sapiens Human MAGPRPSPWARLLLAALISVSLSGTLANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRRCNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFELEVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYRTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSNIVELLEEAFNRIRSNLDIRALDSPRGLRTEVTSKMFQKTRTGSFHIRRGEVGIYQVQLRALEHVDGTHVCQLPEDQKGNIHLKPSFSDGLKMDAGIICDVCTCELQKEVRSARCSFNGDFVCGQCVCSEGWSGQTCNCSTGSLSDIQPCLREGEDKPCSGRGECQCGHCVCYGEGRYEGQFCEYDNFQCPRTSGFLCNDRGRCSMGQCVCEPGWTGPSCDCPLSNATCIDSNGGICNGRGHCECGRCHCHQQSLYTDTICEINYSAIHPGLCEDLRSCVQCQAWGTGEKKGRTCEECNFKVKMVDELKRAEEVVVRCSFRDEDDDCTYSYTMEGDGAPGPNSTVLVHKKKDCPPGSFWWLIPLLLLLLPLLALLLLLCWKYCACCKACLALLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDVVRWKVTNNMQRPGFATHAASINPTELVPYGLSLRLARLCTENLLKPDTRECAQLRQEVEENLNEVYRQISGVHKLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKPALLKLTEKQVEQRAFHDLKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPAGTATLGRRLVNITIIKEQARDVVSFEQPEFSVSRGDQVARIPVIRRVLDGGKSQVSYRTQDGTAQGNRDYIPVEGELLFQPGEAWKELQVKLLELQEVDSLLRGRQVRRFHVQLSNPKFGAHLGQPHSTTIIIRDPDELDRSFTSQMLSSQPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPSGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNDDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYDSFLMYSDDVLRSPSGSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSSDAEAPHGPPDDGGAGGKGGSLPRSATPGPPGEHLVNGRMDFAFPGSTNSLHRMTTTSAAAYGTHLSPHVPHRVLSTSSTLTRDYNSLTRSEHSHSTTLPRDYSTLTSVSSHDSRLTAGVPDTPTRLVFSALGPTSLRVSWQEPRCERPLQGYSVEYQLLNGGELHRLNIPNPAQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDIVGYLVTCEMAQGGGPATAFRVDGDSPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQDGGPFPQLGSRAGLFQHPLQSEYSSITTTHTSATEPFLVDGLTLGAQHLEAGGSLTRHVTQEFVSRTLTTSGTLSTHMDQQFFQT s1pr2_human Sphingosine 1-phosphate receptor 2 S1PR2 EDG5 Endothelial differentiation G-protein coupled receptor 5 Sphingosine 1-phosphate receptor Edg-5 protein protein human Homo sapiens Human MGSLYSEYLNPNKVQEHYNYTKETLETQETTSRQVASAFIVILCCAIVVENLLVLIAVARNSKFHSAMYLFLGNLAASDLLAGVAFVANTLLSGSVTLRLTPVQWFAREGSAFITLSASVFSLLAIAIERHVAIAKVKLYGSDKSCRMLLLIGASWLISLVLGGLPILGWNCLGHLEACSTVLPLYAKHYVLCVVTIFSIILLAIVALYVRIYCVVRSSHADMAAPQTLALLKTVTIVLGVFIVCWLPAFSILLLDYACPVHSCPILYKAHYFFAVSTLNSLLNPVIYTWRSRDLRREVLRPLQCWRPGVGVQGRRRGGTPGHHLLPLRSSSSLERGMHMPTSPTFLEGNTVV s1pr3_human Sphingosine 1-phosphate receptor 3 S1PR3 EDG3 Endothelial differentiation G-protein coupled receptor 3 Sphingosine 1-phosphate receptor Edg-3 protein protein human Homo sapiens Human MATALPPRLQPVRGNETLREHYQYVGKLAGRLKEASEGSTLTTVLFLVICSFIVLENLMVLIAIWKNNKFHNRMYFFIGNLALCDLLAGIAYKVNILMSGKKTFSLSPTVWFLREGSMFVALGASTCSLLAIAIERHLTMIKMRPYDANKRHRVFLLIGMCWLIAFTLGALPILGWNCLHNLPDCSTILPLYSKKYIAFCISIFTAILVTIVILYARIYFLVKSSSRKVANHNNSERSMALLRTVVIVVSVFIACWSPLFILFLIDVACRVQACPILFKAQWFIVLAVLNSAMNPVIYTLASKEMRRAFFRLVCNCLVRGRGARASPIQPALDPSRSKSSSSNNSSHSPKVKEDLPHTAPSSCIMDKNAALQNGIFCN s1pr1_human Sphingosine 1-phosphate receptor 1 S1PR1 CHEDG1 EDG1 Endothelial differentiation G-protein coupled receptor 1 Sphingosine 1-phosphate receptor Edg-1 protein protein human Homo sapiens Human MGPTSVPLVKAHRSSVSDYVNYDIIVRHYNYTGKLNISADKENSIKLTSVVFILICCFIILENIFVLLTIWKTKKFHRPMYYFIGNLALSDLLAGVAYTANLLLSGATTYKLTPAQWFLREGSMFVALSASVFSLLAIAIERYITMLKMKLHNGSNNFRLFLLISACWVISLILGGLPIMGWNCISALSSCSTVLPLYHKHYILFCTTVFTLLLLSIVILYCRIYSLVRTRSRRLTFRKNISKASRSSEKSLALLKTVIIVLSVFIACWAPLFILLLLDVGCKVKTCDILFRAEYFLVLAVLNSGTNPIIYTLTNKEMRRAFIRIMSCCKCPSGDSAGKFKRPIIAGMEFSRSKSDNSSHPQKDEGDNPETIMSSGNVNSSS s1pr2-itgb4 1778912 n/a 1778914 unspecified role unspecified role prey prey n/a 1778915 unspecified role unspecified role bait bait physical association physical association false false false Figure 2 A, 4B S1P -Sphingosine 1-phosphate HGF -hepatocyte growth factor siRNA specific for S1PR1 significantly attenuated S1P-induced increases in this association. itgb4-s1pr2 1778912 n/a 1778914 unspecified role unspecified role bait bait n/a 1778915 unspecified role unspecified role prey prey physical association physical association false false false Figure 2 B, 4B S1P -Sphingosine 1-phosphate HGF -hepatocyte growth factor siRNA specific for S1PR1 significantly attenuated S1P-induced increases in this association. itgb4-s1pr2-1 1778912 n/a 1778914 unspecified role unspecified role bait bait n/a 1778916 unspecified role unspecified role prey prey physical association physical association false false false Figure 2 D, 4C S1P -Sphingosine 1-phosphate HGF -hepatocyte growth factor s1pr2-itgb4-1 1778912 n/a 1778914 unspecified role unspecified role prey prey n/a 1778916 unspecified role unspecified role bait bait physical association physical association false false false Figure 2C, 4C S1P -Sphingosine 1-phosphate HGF -hepatocyte growth factor itgb4-s1pr3 1778913 n/a 1778916 unspecified role unspecified role neutral component neutral component n/a 1778914 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false Figure3 S1P -Sphingosine 1-phosphate HGF -hepatocyte growth factor siRNA specific for S1PR3 itgb4-s1pr3-1 1778913 n/a 1778915 unspecified role unspecified role neutral component neutral component n/a 1778917 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 5 S1P -Sphingosine 1-phosphate HGF -hepatocyte growth factor