IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ teo-2014-2 Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. teo-2014-3 Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. teo-2014-4 Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. teo-2014-1 Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma tap tandem affinity purification TAP TAP tap sequence tag sequence tag identification sequence tag Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins The two affinity tags used are: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP). Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. teo-2014-5 Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma scanning electron microscopy scanning electron microscopy sem sem antibody detection identification by antibody antibody detection Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. teo-2014-6 Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. teo-2014-7 Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Teo C.S., Chu J.J. Virus - Publications including interactions involving viral proteins Viral strain is given as:-DENV-2 NGC strain (NCBI accession no. NC_001474.2). This corresponds to only one Uniprot Genome polyprotein = P14340. miccjh@nus.edu.sg J. Virol. (0022-538X) 2014 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. p14340-pro_0000037966 Non-structural protein 4A protein protein den2n Dengue virus type 2 (strain Thailand/NGS-C/1944) (DENV-2) SLTLNLITEMGRLPTFMTQKARDALDNLAVLHTAEAGGRAYNHALSELPETLETLLLLTLLATVTGGIFLFLMSGRGIGKMTLGMCCIITASILLWYAQIQPHWIAASIILEFFLIVLLIPEPEKQR 2094 2220 vime_human Vimentin VIM protein protein human Homo sapiens Human MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE enoa_human Alpha-enolase ENO1 ENO1L1 MBPB1 MPB1 2-phospho-D-glycerate hydro-lyase Non-neural enolase Enolase 1 Phosphopyruvate hydratase C-myc promoter-binding protein MBP-1 MPB-1 Plasminogen-binding protein protein protein human Homo sapiens Human MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK kpym_human Pyruvate kinase PKM PKM Cytosolic thyroid hormone-binding protein OIP3 Opa-interacting protein 3 PK2 PK3 PKM2 Pyruvate kinase 2/3 Pyruvate kinase muscle isozyme Thyroid hormone-binding protein 1 Tumor M2-PK p58 protein protein human Homo sapiens Human MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP tbb5_human Tubulin beta chain TUBB Tubulin beta-5 chain TUBB5 OK/SW-cl.56 protein protein human Homo sapiens Human MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA g6pd_human Glucose-6-phosphate 1-dehydrogenase G6PD protein protein human Homo sapiens Human MAEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL gdib_human Rab GDP dissociation inhibitor beta Guanosine diphosphate dissociation inhibitor 2 GDI2 RABGDIB protein protein human Homo sapiens Human MNEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESASITPLEDLYKRFKIPGSPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVTEGSFVYKGGKIYKVPSTEAEALASSLMGLFEKRRFRKFLVYVANFDEKDPRTFEGIDPKKTTMRDVYKKFDLGQDVIDFTGHALALYRTDDYLDQPCYETINRIKLYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPIEEIIVQNGKVIGVKSEGEIARCKQLICDPSYVKDRVEKVGQVIRVICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISFAHNVAAQGKYIAIVSTTVETKEPEKEIRPALELLEPIEQKFVSISDLLVPKDLGTESQIFISRTYDATTHFETTCDDIKNIYKRMTGSEFDFEEMKRKKNDIYGED actb_human Actin, cytoplasmic 1 ACTB Beta-actin protein protein human Homo sapiens Human MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF ef1a1_human Elongation factor 1-alpha 1 EEF1A1 EEF1A EF1A LENG7 Eukaryotic elongation factor 1 A-1 Elongation factor Tu Leukocyte receptor cluster member 7 protein protein human Homo sapiens Human MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK p14340_pro-actb-1 1572186 n/a 1572193 unspecified role unspecified role bait bait n/a 1572194 unspecified role unspecified role prey prey physical association physical association false false false Figure 1B vim-p14340_pro 1572186 n/a 1572193 unspecified role unspecified role prey prey n/a 1572194 unspecified role unspecified role bait bait physical association physical association false false false Figure 1B p14340_pro-vim-1 1572187 n/a 1572194 unspecified role unspecified role neutral component neutral component n/a 1572193 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 2,3,9, 10. Colocalization of vimentin with NS4A was lost upon knockdown of CaMKII-gamma. KN93 -a specific CaMK II inhibitor p14340_pro-vim-2 1572187 n/a 1572193 tag visualisation tag visualisation unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1572194 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 5 p14340_pro-vim 1572188 n/a 1572194 unspecified role unspecified role neutral component neutral component n/a 1572193 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 3D p14340_pro-vim-3 1572188 n/a 1572193 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1572194 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 6 p14340_pro-actb 1572189 n/a 1572195 unspecified role unspecified role prey prey n/a 1572196 unspecified role unspecified role prey prey n/a 1572197 unspecified role unspecified role prey prey n/a 1572198 unspecified role unspecified role prey prey n/a 1572194 unspecified role unspecified role prey prey n/a 1572199 unspecified role unspecified role prey prey n/a 1572200 unspecified role unspecified role prey prey n/a 1572201 unspecified role unspecified role prey prey n/a 1572193 unspecified role unspecified role bait bait region tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Table 1, Figure 1 vim-p14340_pro-3 1572190 n/a 1572194 unspecified role unspecified role neutral component neutral component n/a 1572193 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 4 p14340_pro-vim-5 1572191 n/a 1572194 unspecified role unspecified role prey prey n/a 1572193 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6D vim-p14340_pro-4 1572192 n/a 1572194 unspecified role unspecified role bait bait n/a 1572193 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 6D