IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ brockmann-2013-2 Small molecule inhibitors of aurora-a induce proteasomal degradation of N-myc in childhood neuroblastoma. human-imr_32 Homo sapiens:Neuroblastoma cell line imr_32 Human IMR-32 Neuroblastoma Human neuroblastoma cell line established from an abdominal mass (diagnosed as neuroblastoma) of a 1-year-old Caucasian boy in 1967. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Brockmann M., Poon E., Berry T., Carstensen A., Deubzer H.E., Rycak L., Jamin Y., Thway K., Robinson S.P., Roels F., Witt O., Fischer M., Chesler L., Eilers M. Affinomics - Interactions curated for the Affinomics consortium. The authors have confirmed that Figure Supp 2A was carried out in IMR-32 cells. louis.chesler@icr.ac.uk, martin.eilers@biozentrum.uni-wuerzburg.de Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer brockmann-2013-3 Small molecule inhibitors of aurora-a induce proteasomal degradation of N-myc in childhood neuroblastoma. human-sh_ep Homo sapiens SH-EP: human neuroblastoma cell line sh_ep SH-EP human neuroblastoma cell line This is one of two morphologically distinct cell types resulting from the subcloning of the SK-N-SH NB cell line: SH-SY5Y (N-type) and SH-EP (S-type); SH-EP (SHEP) is an Epithelial-like clone. (The first reference to it I found was in 1978. There are no Cabri or ATCC reference numbers.) pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Brockmann M., Poon E., Berry T., Carstensen A., Deubzer H.E., Rycak L., Jamin Y., Thway K., Robinson S.P., Roels F., Witt O., Fischer M., Chesler L., Eilers M. Affinomics - Interactions curated for the Affinomics consortium. The authors have confirmed that Figure Supp 2A was carried out in IMR-32 cells. louis.chesler@icr.ac.uk, martin.eilers@biozentrum.uni-wuerzburg.de Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer brockmann-2013-1 Small molecule inhibitors of aurora-a induce proteasomal degradation of N-myc in childhood neuroblastoma. human-sms-kcn Human SMS-KCN - N-Myc amplified Neuroblastoma cell line sms-kcn SMS-KCN - N-Myc amplified Neuroblastoma cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Brockmann M., Poon E., Berry T., Carstensen A., Deubzer H.E., Rycak L., Jamin Y., Thway K., Robinson S.P., Roels F., Witt O., Fischer M., Chesler L., Eilers M. Affinomics - Interactions curated for the Affinomics consortium. The authors have confirmed that Figure Supp 2A was carried out in IMR-32 cells. louis.chesler@icr.ac.uk, martin.eilers@biozentrum.uni-wuerzburg.de Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer brockmann-2013-4 Small molecule inhibitors of aurora-a induce proteasomal degradation of N-myc in childhood neuroblastoma. mouse-neuroblastoma Mouse Neuroblastoma neuroblastoma Neuroblastoma ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Brockmann M., Poon E., Berry T., Carstensen A., Deubzer H.E., Rycak L., Jamin Y., Thway K., Robinson S.P., Roels F., Witt O., Fischer M., Chesler L., Eilers M. Affinomics - Interactions curated for the Affinomics consortium. Transgenic Mice with the Human MYCN transgene, which is overexpressed in neuroectodermal cells, resulting in the development of Neuroblastoma. The authors have confirmed that Figure Supp 2A was carried out in IMR-32 cells. louis.chesler@icr.ac.uk, martin.eilers@biozentrum.uni-wuerzburg.de Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer brockmann-2013-5 Small molecule inhibitors of aurora-a induce proteasomal degradation of N-myc in childhood neuroblastoma. human-imr_5 Homo sapiens IMR-5:neuroblastoma cell line imr_5 Human IMR-5 Neuroblastoma cell line Morphology: neuroblastic-like, grown as monolayer. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Brockmann M., Poon E., Berry T., Carstensen A., Deubzer H.E., Rycak L., Jamin Y., Thway K., Robinson S.P., Roels F., Witt O., Fischer M., Chesler L., Eilers M. Affinomics - Interactions curated for the Affinomics consortium. The authors have confirmed that Figure Supp 2A was carried out in IMR-32 cells. louis.chesler@icr.ac.uk, martin.eilers@biozentrum.uni-wuerzburg.de Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer brockmann-2013-6 Small molecule inhibitors of aurora-a induce proteasomal degradation of N-myc in childhood neuroblastoma. human-sh_ep Homo sapiens SH-EP: human neuroblastoma cell line sh_ep SH-EP human neuroblastoma cell line This is one of two morphologically distinct cell types resulting from the subcloning of the SK-N-SH NB cell line: SH-SY5Y (N-type) and SH-EP (S-type); SH-EP (SHEP) is an Epithelial-like clone. (The first reference to it I found was in 1978. There are no Cabri or ATCC reference numbers.) anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Brockmann M., Poon E., Berry T., Carstensen A., Deubzer H.E., Rycak L., Jamin Y., Thway K., Robinson S.P., Roels F., Witt O., Fischer M., Chesler L., Eilers M. Affinomics - Interactions curated for the Affinomics consortium. The authors have confirmed that Figure Supp 2A was carried out in IMR-32 cells. louis.chesler@icr.ac.uk, martin.eilers@biozentrum.uni-wuerzburg.de Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer max_human Protein max MAX BHLHD4 Class D basic helix-loop-helix protein 4 Myc-associated factor X protein protein human Homo sapiens Human MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS mycn_human N-myc proto-oncogene protein MYCN NMYC BHLHE37 Class E basic helix-loop-helix protein 37 protein protein human Homo sapiens Human MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC apex1_mouse_gene APEX1_MOUSE_GENE gene gene mouse Mus musculus Mouse aurka_mouse Aurora kinase A Serine/threonine-protein kinase Ayk1 Aurka Aik Airk Ark1 Aura Ayk1 Btak Iak1 Stk15 Stk6 Aurora 2 Aurora family kinase 1 Aurora/IPL1-related kinase 1 Ipl1- and aurora-related kinase 1 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein mouse Mus musculus Mouse MDRCKENCVSRPVKTTVPFGPKRVLVTEQIPSQNLGSASSGQAQRVLCPSNSQRVPSQAQKLGAGQKPAPKQLPAASVPRPVSRLNNPQKNEQPAASGNDSEKEQASLQKTEDTKKRQWTLEDFDIGRPLGKGKFGNVYLARERQSKFILALKVLFKTQLEKANVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSNGELKIADFGWSVHAPSSRRTTMCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGMPPFEAHTYQETYRRISRVEFTFPDFVTEGARDLISRLLKHNASQRLTLAEVLEHPWIKANSSKPPTGHTSKEPTSKSS mycn-aurka-1 1426719 n/a 1426725 unspecified role unspecified role prey prey n/a 1426726 unspecified role unspecified role prey prey n/a 1426727 unspecified role unspecified role bait bait association association false false false 2A/B/E Supp 2A, Supp 3A MLN8054 and MLN8237 - Aurora-A inhibitors Addition of MLN8054 or MLN8237 decreased the amount of Aurora-A that coimmunoprecipitated with N-Myc; however MLN8054 had no effect on the amount of Max that bound to N-Myc. aurka-max 1426719 n/a 1426727 unspecified role unspecified role prey prey n/a 1426726 unspecified role unspecified role bait bait physical association physical association false false false 2C MLN8237 - Aurora-A inhibitor max-mycn 1426720 n/a 1426725 unspecified role unspecified role neutral component neutral component n/a 1426727 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 2D aurka-mycn-3 1426720 n/a 1426726 unspecified role unspecified role neutral component neutral component n/a 1426727 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 2D MLN8237 - Aurora-A inhibitor aurka-mycn-2 1426721 n/a 1426726 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1426727 unspecified role unspecified role prey prey physical association physical association false false false 3C MLN8054 -an Aurora-A inhibitor The N-Myc/Aurora-AT217D complex remained stable in the presence of MLN8054 mycn-apex1 1426722 n/a 1426728 unspecified role unspecified role prey prey promoter_e_box binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1426727 unspecified role unspecified role bait bait association association false false false 6B MLN8054 - an Aurora-A-specific inhibitor aurka-max-1 1426723 n/a 1426726 unspecified role unspecified role bait bait n/a 1426727 unspecified role unspecified role prey prey physical association physical association false false false Supp 2B aurka-mycn-4 1426724 n/a 1426729 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1426727 unspecified role unspecified role prey prey physical association physical association false false false Supp 5A