IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gruszczynska_biegala-2013-1 Native STIM2 and ORAI1 proteins form a calcium-sensitive and thapsigargin-insensitive complex in cortical neurons. rat-cortical_neuron Rat cortical Neurons brain-cortex_neuron Cerebral cortical neuron anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gruszczynska-Biegala J., Kuznicki J. Affinomics - Interactions curated for the Affinomics consortium. jkuznicki@iimcb.gov.pl J. Neurochem. (0022-3042) 2013 Only protein-protein interactions imex curation gruszczynska_biegala-2013-2 Native STIM2 and ORAI1 proteins form a calcium-sensitive and thapsigargin-insensitive complex in cortical neurons. rat-cortical_neuron Rat cortical Neurons brain-cortex_neuron Cerebral cortical neuron pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Gruszczynska-Biegala J., Kuznicki J. Affinomics - Interactions curated for the Affinomics consortium. jkuznicki@iimcb.gov.pl J. Neurochem. (0022-3042) 2013 Only protein-protein interactions imex curation d4a5x1_rat Stim2 protein protein rat Rattus norvegicus (Rat) Rat MLLFGLLVAGVADGCDLVPRHLRGRRASGSAGAAASPSAAAAGERQALLTDPCMSLSPPCFTEEDRFSLEALQTIHKQMDDDKDGGIEVDESDEFIREDMKYKDATNKHSHLHREDKHITVEDLWKQWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHETSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTISIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRTVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSLYSDHSWVVMPRVSIPPYPIAGGVDDLDEDTPPIVSQFPGTVAKPAGSLARSSSLCRSRRSIVPSSPQSQRAQLPAHAPLAAHPRHPHHPQHSLPSPDPDILSVSSCPALYRNEEEEEAIYFTAEKQWEVPDTASECDSLNSSIGRKQSPPSSLEIYQTLSSRKISRDELSLEDSSRGDSPVTADVSRGSPECVGLTETKSMIFSPASRVYNGILEKSCSMHQLSSGIPVPNPRHTSCSLAGSDSKPVQEASSVSRVGSIPHDLCHNGEKSKKPSKIKSLFKKKSK crcm1_rat Calcium release-activated calcium channel protein 1 Orai1 Cracm1 Tmem142a Protein orai-1 Transmembrane protein 142A protein protein rat Rattus norvegicus (Rat) Rat MHPEPAPPPNNSNPELPLSGGSSTSGSRRSRRRSGDGEPTGAPPLPPPPAVSYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDTDHDYPPGLLIVFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKRQAGQPSPTKPPTEPAVVVANSSNNGGITPGEAAAIASTAIMVPCGLVFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHSLTPGTHYA stim1_rat Stromal interaction molecule 1 Stim1 Sim protein protein rat Rattus norvegicus (Rat) Rat MDVCARLALWLLWGLLLHQGQSLSHSHSEKNTGASSGATSEESTEAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKASEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHTSDRQRVAPKPPQMGRAADEALNATSSNGSHRLIEGVHPGSLVEKLPDSPALAKKTILALNHGLDKAHSLMELNPSVPPGGSPLLDSSHSHSPSSPDPDTPSPVGDSRALQGSRNTRIPHLAGKKAMAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK stim2-orai1 1567173 n/a 1567175 unspecified role unspecified role bait bait n/a 1567176 unspecified role unspecified role prey prey physical association physical association false false false 1 EGTA (removes calcium ions) orai1-stim2 1567173 n/a 1567176 unspecified role unspecified role bait bait n/a 1567175 unspecified role unspecified role prey prey physical association physical association false false false 1 EGTA (removes calcium ions) orai1-stim2-2 1567174 n/a 1567175 unspecified role unspecified role neutral component neutral component n/a 1567176 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 2,3,4,5, Supp fig 1 EGTA containing medium (chelates/removes calcium ions). high-calcium ion containing medium Results suggest that STIM2-ORAI1 complexes formed as a result of decreased cytosolic calcium levels. BAPTA-AM - a calcium chelator that penetrates live cells and decreases intracellular calcium concentrations Low extracellular calcium levels. orai1-stim2-3 1567174 n/a 1567176 unspecified role unspecified role neutral component neutral component n/a 1567177 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 6 Thapsigargin (a non-competitive inhibitor of sarco/endoplasmic reticulum Ca2+ ATPases resulting in increased cytosolic (intracellular) calcium concentration. The addition of the SOCE (store-operated calcium entry) inhibitor ML9 (1-(5-Chloronaphthalenesulfonyl)homopiperazine hydrochloride) after addition of Thapsigargin decreased the number of complexes nearly to the state before thapsigargin stimulation.