UniProt UniProt http://www.uniprot.org zhang-2012j-2 Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Traffic (1398-9219) 2012 mimix curation zhang-2012j-3 Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Traffic (1398-9219) 2012 mimix curation zhang-2012j-1 Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Traffic (1398-9219) 2012 mimix curation zhang-2012j-4 Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Traffic (1398-9219) 2012 mimix curation zhang-2012j-6 Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Traffic (1398-9219) 2012 mimix curation zhang-2012j-7 Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Zhang J., Reiling C., Reinecke J.B., Prislan I., Marky L.A., Sorgen P.L., Naslavsky N., Caplan S. Affinomics - Interactions curated for the Affinomics consortium. Origin of EHD1 (for GST-EH1) according ref. 4 (pmid 12032069 and ref 42 (pmid 15020713). Interactions involving Vps26 not included as the used antibodies might not differentiate between VPS26A and VPS26B. scaplan@unmc.edu Traffic (1398-9219) 2012 mimix curation ehd1_human EH domain-containing protein 1 Testilin CDABP0131 EHD1 PAST PAST1 PAST homolog 1 protein protein human Homo sapiens Human MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE anfy1_human Rabankyrin-5 Ankyrin repeat and FYVE domain-containing protein 1 ANKFY1 ANKHZN KIAA1255 Ankyrin repeats hooked to a zinc finger motif protein protein human Homo sapiens Human MAEEEVAKLEKHLMLLRQEYVKLQKKLAETEKRCALLAAQANKESSSESFISRLLAIVADLYEQEQYSDLKIKVGDRHISAHKFVLAARSDSWSLANLSSTKELDLSDANPEVTMTMLRWIYTDELEFREDDVFLTELMKLANRFQLQLLRERCEKGVMSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMSAQLLYKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNEADHNGDLALDLALSRRLESIATTLVSHKADVDMVDKSGWSLLHKGIQRGDLFAATFLIKNGAFVNAATLGAQETPLHLVALYSSKKHSADVMSEMAQIAEALLQAGANPNMQDSKGRTPLHVSIMAGNEYVFSQLLQCKQLDLELKDHEGSTALWLAVQHITVSSDQSVNPFEDVPVVNGTSFDENSFAARLIQRGSHTDAPDTATGNCLLQRAAGAGNEAAALFLATNGAHVNHRNKWGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPLPKEAASLTSLADSVHLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGAAINDTMSDGQTLLHMAIQRQDSKSALFLLEHQADINVRTQDGETALQLAIRNQLPLVVDAICTRGADMSVPDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPGGCLQTLLHRAIDENNEPTACFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPIHVAISSQHGVIIQLLVSHPDIHLNVRDRQGLTPFACAMTFKNNKSAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVHANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPGYPLDKPDADGSTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDGSYCYECTARFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVCNICFDVLTLGGVS milk1_human MICAL-like protein 1 MICALL1 KIAA1668 MIRAB13 Molecule interacting with Rab13 protein protein human Homo sapiens Human MAGPRGALLAWCRRQCEGYRGVEIRDLSSSFRDGLAFCAILHRHRPDLLDFDSLSKDNVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFCSPGQAGVSPPRKGLAPCSPPSVAPTPVEPEDVAQGEELSSGSLSEQGTGQTPSSTCAACQQHVHLVQRYLADGRLYHRHCFRCRRCSSTLLPGAYENGPEEGTFVCAEHCARLGPGTRSGTRPGPFSQPKQQHQQQLAEDAKDVPGGGPSSSAPAGAEADGPKASPEARPQIPTKPRVPGKLQELASPPAGRPTPAPRKASESTTPAPPTPRPRSSLQQENLVEQAGSSSLVNGRLHELPVPKPRGTPKPSEGTPAPRKDPPWITLVQAEPKKKPAPLPPSSSPGPPSQDSRQVENGGTEEVAQPSPTASLESKPYNPFEEEEEDKEEEAPAAPSLATSPALGHPESTPKSLHPWYGITPTSSPKTKKRPAPRAPSASPLALHASRLSHSEPPSATPSPALSVESLSSESASQTAGAELLEPPAVPKSSSEPAVHAPGTPGNPVSLSTNSSLASSGELVEPRVEQMPQASPGLAPRTRGSSGPQPAKPCSGATPTPLLLVGDRSPVPSPGSSSPQLQVKSSCKENPFNRKPSPAASPATKKATKGSKPVRPPAPGHGFPLIKRKVQADQYIPEEDIHGEMDTIERRLDALEHRGVLLEEKLRGGLNEGREDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQRQADVEYELRCLLNKPEKDWTEEDRAREKVLMQELVTLIEQRNAIINCLDEDRQREEEEDKMLEAMIKKKEFQREAEPEGKKKGKFKTMKMLKLLGNKRDAKSKSPRDKS vps35_human Vacuolar protein sorting-associated protein 35 Vesicle protein sorting 35 Maternal-embryonic 3 VPS35 MEM3 TCCCTA00141 protein protein human Homo sapiens Human MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL f1md79_bovin ANKFY1 protein protein bovin Bos taurus (Bovine) Bovine MKEVAKLEKHLMLLRQEYVKLQKKLAETEKRCTLLAAQAKKESSSESFISRLLTIVADLYEQEQYSDLKIKVGSRHINAHKFVLAARSDSWSLASLSSTEELDLSDANPEVTMTMLRWIYTDELEFKEDDVFLTELMKLANRFQLQLLRERCEKGVMSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMSAQLLYKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNELDHNGDLALDLALSRRLESIATTLVNHKADVDMVDKNGWSLLHKGIQRGDLFAATFLIKNGALVNAATLGAQETPLHLVASYNSKKHSAAVMSEMAQLTEALLQAGANPNMQDSKGRTPLHLSIMARNEYVFNQLLQCKQLDLELKDHEGSTALWLAVQYITVSSDQSVNPFEDLPVVNGTSFDENSFAARLIQRGSNTNAPDTVTGNCLLQRAAEAGNEAAALFLATNGAHVNHRNKWGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPSPKEAMSLPSSADSSVYLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGACINDTMSDGQTLLHMAMQRQDSKSALFLLEHQADINVRTQDGETALQLAIRNQLPLVVDAICTRGADMSVPDEQGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPSGCLQTLLHRAIDENNEPITCFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPVHVAISNQHSVIIQLLISHPDIHLNVRDRQGLTPFACAMTYKNNKAAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVQANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPEYPLDKPDAEGNTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDGSNCYECTAKFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVCNICFDVLTLGGVS ehd1-ankfy1-1 1566321 n/a 1566327 unspecified role unspecified role bait bait n/a 1566328 unspecified role unspecified role prey prey physical association physical association false false false 1 c ankfy1-ehd1-4 1566321 n/a 1566327 unspecified role unspecified role prey prey n/a 1566328 unspecified role unspecified role bait bait physical association physical association false false false 1b ankfy1-ehd1 1566322 n/a 1566327 unspecified role unspecified role prey prey n/a 1566328 unspecified role unspecified role bait bait physical association physical association false false false 2c ankfy1-micall1 1566322 n/a 1566329 unspecified role unspecified role prey prey n/a 1566328 unspecified role unspecified role bait bait n/a 1566330 unspecified role unspecified role prey prey association association false false false 4e VPS26A and/or VPS26B detected in the precipitate. ehd1-ankfy1 1566323 n/a 1566327 unspecified role unspecified role bait bait n/a 1566331 unspecified role unspecified role prey prey physical association physical association false false false 1A ankfy1-ehd1-2 1566324 n/a 1566327 unspecified role unspecified role neutral component neutral component n/a 1566328 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2d ankfy1-ehd1-3 1566325 n/a 1566327 unspecified role unspecified role neutral component neutral component n/a 1566328 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false table 1 ehd1-micall1 1566326 n/a 1566329 unspecified role unspecified role neutral component neutral component n/a 1566327 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 5c