IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kiel-2011-1 Structural and functional protein network analyses predict novel signaling functions for rhodopsin. pig-retina-rod_outer_segment Pig Rod Outer Segment retina-rod_outer_segment rod outer segment bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Kiel C., Vogt A., Campagna A., Chatr-aryamontri A., Swiatek-de Lange M., Beer M., Bolz S., Mack A.F., Kinkl N., Cesareni G., Serrano L., Ueffing M. Affinomics - Interactions curated for the Affinomics consortium. Two dimensional electrophoresis: BN page followed by SDS-PAGE. cesareni@uniroma2.it,luis.serrano@crg.eu,marius.ueffing@uni-tuebingen.de Mol. Syst. Biol. 2011 Only protein-protein interactions imex curation kiel-2011-3 Structural and functional protein network analyses predict novel signaling functions for rhodopsin. pig-retina-rod_outer_segment Pig Rod Outer Segment retina-rod_outer_segment rod outer segment density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Kiel C., Vogt A., Campagna A., Chatr-aryamontri A., Swiatek-de Lange M., Beer M., Bolz S., Mack A.F., Kinkl N., Cesareni G., Serrano L., Ueffing M. Affinomics - Interactions curated for the Affinomics consortium. Rho ABC was found in this complex, but cannot be identified due to lack of antibody specificity. "Arrestin" was found in this complex, but also cannot be identified. cesareni@uniroma2.it,luis.serrano@crg.eu,marius.ueffing@uni-tuebingen.de Mol. Syst. Biol. 2011 Only protein-protein interactions imex curation kiel-2011-4 Structural and functional protein network analyses predict novel signaling functions for rhodopsin. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kiel C., Vogt A., Campagna A., Chatr-aryamontri A., Swiatek-de Lange M., Beer M., Bolz S., Mack A.F., Kinkl N., Cesareni G., Serrano L., Ueffing M. Affinomics - Interactions curated for the Affinomics consortium. Rho ABC was found in this complex, but cannot be identified due to lack of antibody specificity. cesareni@uniroma2.it,luis.serrano@crg.eu,marius.ueffing@uni-tuebingen.de Mol. Syst. Biol. 2011 Only protein-protein interactions imex curation kiel-2011-5 Structural and functional protein network analyses predict novel signaling functions for rhodopsin. pig-retina-rod_outer_segment Pig Rod Outer Segment retina-rod_outer_segment rod outer segment anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kiel C., Vogt A., Campagna A., Chatr-aryamontri A., Swiatek-de Lange M., Beer M., Bolz S., Mack A.F., Kinkl N., Cesareni G., Serrano L., Ueffing M. Affinomics - Interactions curated for the Affinomics consortium. Rho ABC was found in this complex, but cannot be identified due to lack of antibody specificity. cesareni@uniroma2.it,luis.serrano@crg.eu,marius.ueffing@uni-tuebingen.de Mol. Syst. Biol. 2011 Only protein-protein interactions imex curation i3lfi0_pig RAC1 protein protein pig Sus scrofa (Pig) Pig AVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTQDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL The sequence has been withdrawn from uniprot. f1smv2_pig Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta PDE6D protein protein pig Sus scrofa (Pig) Pig MPYSSLICLIRNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV rock2_pig Rho-associated protein kinase 2 ROCK2 Rho kinase 2 Rho-associated, coiled-coil-containing protein kinase 2 Rho-associated, coiled-coil-containing protein kinase II p164 ROCK-2 protein protein pig Sus scrofa (Pig) Pig MSRPPPTGKMPGAPEAVSGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCKENDSIQIRKNEESQEIQKKLYTLEEHLSTEIQAKEELEQKCKSVNTRLEKVAKELEEEIALRKNVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKDFINLQSVLESERRDRTHGSEIINDLQGRISGLEEDLKNGKILLTKVEMEKRQLQERFTDLEKEKNNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSILDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMSLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDTTIASLEETNRTLTSDVANLANEKEELNNKLKDAQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDMRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNCICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAANKPS opsd_pig Rhodopsin RHO RHO1 protein protein pig Sus scrofa (Pig) Pig MNGTEGPNFYVPFSNKTGVVRSPFEYPQYYLAEPWQFSMLAAYMFMLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGLALTWVMALACAAPPLVGWSRYIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFSIPLVIIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVVAFLICWLPYASVAFYIFTHQGSDFGPIFMTIPAFFAKSASIYNPVIYIMMNKQFRNCMLTTLCCGKNPLGDDEASTTTSKTETSQVAPA i3lje2_pig Dihydropyrimidinase-related protein 2 DPYSL2 protein protein pig Sus scrofa (Pig) Pig MSYQGKKNIPRITSDRLLIKGGKIVNDDQSFYADIYMEDGLIKQIGENLIVPGGVKTIEAHSRMVIPGGIDVHTRFQMPDQGMTSADDFFQGTKAALAGGTTMIIDHVVPEPGTSLLSAFDQWREWADSKSCCDYSLHVDITEWHKGIQEEMEALVKDHGVNSFLVYMAFKDRFQLTDSQIYEVLSVIRDIGAIAQVHAENGDIIAEVEAEAVNRSITIANQTNCPLYITKVMSKSAAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDFLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSIIWDKAVVTGKMDENQFVAVTSTNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLEDGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAKQQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG i3la61_pig GNAT1 protein protein pig Sus scrofa (Pig) Pig GAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFERASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFSEKIKKAHLSICFPDYNGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF cona_canen Concanavalin-A protein protein canen Canavalia ensiformis (Jack bean) Horse bean MAISKKSSLFLPIFTFITMFLMVVNKVSSSTHETNALHFMFNQFSKDQKDLILQGDATTGTDGNLELTRVSSNGSPQGSSVGRALFYAPVHIWESSAVVASFEATFTFLIKSPDSHPADGIAFFISNIDSSIPSGSTGRLLGLFPDANVIRNSTTIDFNAAYNADTIVAVELDTYPNTDIGDPSYPHIGIDIKSVRSKKTAKWNMQNGKVGTAHIIYNSVDKRLSAVVSYPNADSATVSYDVDLDNVLPEWVRVGLSASTGLYKETNTILSWSFTSKLKSNEIPDIATVV pde6d-rac1 1496467 n/a 1496471 unspecified role unspecified role neutral component neutral component n/a 1496472 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 7A dpysl2-gnat1 1496468 n/a 1496471 unspecified role unspecified role neutral component neutral component n/a 1496473 unspecified role unspecified role neutral component neutral component n/a 1496474 unspecified role unspecified role neutral component neutral component n/a 1496475 unspecified role unspecified role neutral component neutral component n/a 1496476 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp 6A cona-dpysl2-2 1496469 n/a 1496475 unspecified role unspecified role prey prey pig-retina-rod_outer_segment Pig Rod Outer Segment retina-rod_outer_segment rod outer segment n/a 1496477 unspecified role unspecified role bait bait n/a 1496474 unspecified role unspecified role prey prey pig-retina-rod_outer_segment Pig Rod Outer Segment retina-rod_outer_segment rod outer segment n/a 1496476 unspecified role unspecified role prey prey pig-retina-rod_outer_segment Pig Rod Outer Segment retina-rod_outer_segment rod outer segment association association false false false Supp 6C AMM:-methylmannoside -lowers the affinity of proteins for the beads. cona-dpysl2-3 1496470 n/a 1496476 unspecified role unspecified role prey prey n/a 1496474 unspecified role unspecified role bait bait physical association physical association false false false Supp 6D