IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ borroto_escuela-2012-3 Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation borroto_escuela-2012-1 Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation borroto_escuela-2012-2 Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain pla proximity ligation assay pELISA pELISA PLA tag visualisation tag visualisation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation borroto_escuela-2012-4 Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pla proximity ligation assay pELISA pELISA PLA tag visualisation tag visualisation Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation borroto_escuela-2012-5 Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation borroto_escuela-2012-6 Fibroblast growth factor receptor 1- 5-hydroxytryptamine 1A heteroreceptor complexes and their enhancement of hippocampal plasticity. rat-neuron Rattus norvegicus neuronal cell neuron Neuron [Neuronal cell; Neural cell confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Borroto-Escuela D.O., Romero-Fernandez W., Mudó G., Pérez-Alea M., Ciruela F., Tarakanov A.O., Narvaez M., Di Liberto V., Agnati L.F., Belluardo N., Fuxe K. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that the FGFR1 constructs are of HUMAN origin. Kjell.Fuxe@ki.se Biol. Psychiatry (0006-3223) 2012 Only protein-protein interactions imex curation fgfr1_human Fibroblast growth factor receptor 1 FGFR1 FGFBR FLG FLT2 Fms-like tyrosine kinase 2 Proto-oncogene c-Fgr BFGFR CEK HBGFR Basic fibroblast growth factor receptor 1 N-sam protein protein human Homo sapiens Human MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR 5ht1a_human 5-hydroxytryptamine receptor 1A HTR1A ADRB2RL1 ADRBRL1 G-21 Serotonin receptor 1A protein protein human Homo sapiens Human MDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVKKVEKTGADTRHGASPAPQPKKSVNGESGSRNWRLGVESKAGGALCANGAVRQGDDGAALEVIEVHRVGNSKEHLPLPSEAGPTPCAPASFERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPTLLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRQ 5ht1b_human 5-hydroxytryptamine receptor 1B HTR1B HTR1DB Serotonin receptor 1B Serotonin 1D beta receptor S12 protein protein human Homo sapiens Human MEEPGAQCAPPPPAGSETWVPQANLSSAPSQNCSAKDYIYQDSISLPWKVLLVMLLALITLATTLSNAFVIATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQVVCDFWLSSDITCCTASILHLCVIALDRYWAITDAVEYSAKRTPKRAAVMIALVWVFSISISLPPFFWRQAKAEEEVSECVVNTDHILYTVYSTVGAFYFPTLLLIALYGRIYVEARSRILKQTPNRTGKRLTRAQLITDSPGSTSSVTSINSRVPDVPSESGSPVYVNQVKVRVSDALLEKKKLMAARERKATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHLAIFDFFTWLGYLNSLINPIIYTMSNEDFKQAFHKLIRFKCTS 5ht1a_rat 5-hydroxytryptamine receptor 1A Htr1a 5ht1a Serotonin receptor 1A protein protein rat Rattus norvegicus (Rat) Rat MDVFSFGQGNNTTASQEPFGTGGNVTSISDVTFSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVRKVEKKGAGTSLGTSSAPPPKKSLNGQPGSGDWRRCAENRAVGTPCTNGAVRQGDDEATLEVIEVHRVGNSKEHLPLPSESGSNSYAPACLERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPALLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRR fgfr1_rat Fibroblast growth factor receptor 1 Fgfr1 Flg MFR Proto-oncogene c-Fgr Basic fibroblast growth factor receptor 1 protein protein rat Rattus norvegicus (Rat) Rat MWGWRGLLFWAVLVTATLCTARPAPTLPEQAQPWGVPVEVESLLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVRDSIPADSGLYACVTNSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRRPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPSPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYDQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIIYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPNPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWNAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQDSPSFPDTRSSTCSSGEDSVFSHEPFPEEPCLPRHPTQLANGGLNRR fgfr1-htr1a-4 1374922 n/a 1374928 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1374929 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P08908:p.[Asp406_Asn409delinsAlaAlaAlaAla;Phe411_Lys416delinsAlaAlaAlaAlaAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false proximity proximity close-contact colocalization neighbourhood interaction false false false 1D, 1E, supp 3B, supp 3C, supp 4C. 8-OH-DPAT ((±)-7-Hydroxy 2-dipropylaminotetralin hydrobromide), -a 5-HT1A receptor agonist +FGF2 fgfr1-fgfr1 1374922 n/a 1374929 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P08908:p.[Asp406_Asn409delinsAlaAlaAlaAla;Phe411_Lys416delinsAlaAlaAlaAlaAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1374928 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1374928 competitor competitor competitor neutral component neutral component n/a 1374929 competitor competitor competitor neutral component neutral component h8-mutant_asp406ala,phe407ala,gln408ala,ans409ala,phe411ala,lys412ala,lys413ala,ile414ala,ile415ala,lys416ala sufficient to bind sufficient binding region sufficient to bind alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1374930 competitor competitor competitor neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 1D, 1E, supp 3B, supp 3C, supp 4C 8-OH-DPAT ((±)-7-Hydroxy 2-dipropylaminotetralin hydrobromide), -a 5-HT1A receptor agonist +FGF2 Treatment with FGF2 or 8-OH-DPAT significantly reduced the BRET50 values and thus increased the affinity of the FGFR1–5-HT1A heteroreceptor interaction (Figure 1D, also see Fig Supp 3C). The 5-HT1A-Hx8 mutant showed an increased BRET50 value versus the wild-type but has a reduced ability to form a receptor complex with FGFR1. fgfr1-fgfr1-1 1374922 n/a 1374929 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1374928 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1374929 competitor competitor competitor neutral component neutral component trans_membrane_region_5 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trans_membrane_region_7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false proximity proximity close-contact colocalization neighbourhood interaction false false false 1E fgfr1-htr1a-24 1374923 n/a 1374931 unspecified role unspecified role unspecified role unspecified role n/a 1374932 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false 1B fgfr1-htr1a-2 1374924 n/a 1374931 unspecified role unspecified role neutral component neutral component n/a 1374932 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 1A,1C, Supp1C 8-OH-DPAT ((±)-7-Hydroxy 2-dipropylaminotetralin hydrobromide), -a 5-HT1A receptor agonist +FGF2 fgfr1-htr1a-8 1374925 n/a 1374928 unspecified role unspecified role neutral component neutral component n/a 1374929 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false Supp 1B 8-OH-DPAT ((±)-7-Hydroxy 2-dipropylaminotetralin hydrobromide), -a 5-HT1A receptor agonist +FGF2 fgfr1-htr1a-10 1374926 n/a 1374928 unspecified role unspecified role neutral component neutral component c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1374929 unspecified role unspecified role neutral component neutral component c_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false supp 2B 8-OH-DPAT ((±)-7-Hydroxy 2-dipropylaminotetralin hydrobromide), -a 5-HT1A receptor agonist +FGF2 fgfr1-htr1a-11 1374927 n/a 1374929 unspecified role unspecified role neutral component neutral component c_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1374928 unspecified role unspecified role neutral component neutral component c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false supp 2A