IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ vuoriluoto-2011-2 Spatio-temporal composition of the mitotic Chromosomal Passenger Complex detected using in situ proximity ligation assay. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Vuoriluoto M., Laine L.J., Saviranta P., Pouwels J., Kallio M.J. Affinomics - Interactions curated for the Affinomics consortium. marko.kallio@vtt.fi Mol Oncol (1574-7891) 2011 Only protein-protein interactions imex curation vuoriluoto-2011-1 Spatio-temporal composition of the mitotic Chromosomal Passenger Complex detected using in situ proximity ligation assay. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Vuoriluoto M., Laine L.J., Saviranta P., Pouwels J., Kallio M.J. Affinomics - Interactions curated for the Affinomics consortium. marko.kallio@vtt.fi Mol Oncol (1574-7891) 2011 Only protein-protein interactions imex curation ince_human Inner centromere protein INCENP protein protein human Homo sapiens Human MGTTAPGPIHLLELCDQKLMEFLCNMDNKDLVWLEEIQEEAERMFTREFSKEPELMPKTPSQKNRRKKRRISYVQDENRDPIRRRLSRRKSRSSQLSSRRLRSKDSVEKLATVVGENGSVLRRVTRAAAAAAAATMALAAPSSPTPESPTMLTKKPEDNHTQCQLVPVVEIGISERQNAEQHVTQLMSTEPLPRTLSPTPASATAPTSQGIPTSDEESTPKKSKARILESITVSSLMATPQDPKGQGVGTGRSASKLRIAQVSPGPRDSPAFPDSPWRERVLAPILPDNFSTPTGSRTDSQSVRHSPIAPSSPSPQVLAQKYSLVAKQESVVRRASRRLAKKTAEEPAASGRIICHSYLERLLNVEVPQKVGSEQKEPPEEAEPVAAAEPEVPENNGNNSWPHNDTEIANSTPNPKPAASSPETPSAGQQEAKTDQADGPREPPQSARRKRSYKQAVSELDEEQHLEDEELQPPRSKTPSSPCPASKVVRPLRTFLHTVQRNQMLMTPTSAPRSVMKSFIKRNTPLRMDPKCSFVEKERQRLENLRRKEEAEQLRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEEVEARRKQEEEARRLRWLQQEEEERRHQELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERREQERREQERREQERQLAEQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAERQLQEEQEKKAKEAAGASKALNVTVDVQSPACTSYQMTPQGHRAPPKINPDNYGMDLNSDDSTDDEAHPRKPIPTWARGTPLSQAIIHQYYHPPNLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSPPLQGARVPSSLAYSLKKH borea_human Borealin Dasra-B Cell division cycle-associated protein 8 Pluripotent embryonic stem cell-related gene 3 protein CDCA8 PESCRG3 protein protein human Homo sapiens Human MAPRKGSSRVAKTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK birc5_human Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 BIRC5 API4 IAP4 protein protein human Homo sapiens Human MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD aurkb_human Aurora kinase B AIRK2 Aurora- and IPL1-like midbody-associated protein 1 Aurora/IPL1-related kinase 2 STK-1 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase 5 STK1 STK5 Aurora 1 AURKB AIK2 AIM1 ARK2 STK12 Serine/threonine-protein kinase aurora-B protein protein human Homo sapiens Human MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS birc5-cdca8-16 1435558 n/a 1435560 ancillary ancillary neutral component neutral component n/a 1435561 unspecified role unspecified role neutral component neutral component n/a 1435562 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 2b, 2d, 2e, 3c ZM447439 (aurora B kinase inhibitor), upon MG132 treatment (required to stop cell cycle and make the effect detectable). Interaction found during pro-metaphase and metaphase. aurkb-birc5-17 1435558 n/a 1435562 unspecified role unspecified role neutral component neutral component n/a 1435563 unspecified role unspecified role neutral component neutral component n/a 1435560 ancillary ancillary neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 2d, 2e, 3c ZM447439 (aurora B kinase inhibitor), upon MG132 treatment (required to stop cell cycle and make the effect detectable). Interaction found during pro-metaphase and metaphase. aurkb-borea-18 1435558 n/a 1435563 unspecified role unspecified role neutral component neutral component n/a 1435561 unspecified role unspecified role neutral component neutral component n/a 1435560 ancillary ancillary neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 2d, 2e, 3c ZM447439 (aurora B kinase inhibitor), upon MG132 treatment (required to stop cell cycle and make the effect detectable). Interaction found during pro-metaphase and metaphase. aurkb-cdca8-2 1435558 n/a 1435560 unspecified role unspecified role neutral component neutral component n/a 1435563 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 2d, 2e, 3c ZM447439 (aurora B kinase inhibitor), upon MG132 treatment (required to stop cell cycle and make the effect detectable). Interaction found during pro-metaphase and metaphase. birc5-cdca8-17 1435558 n/a 1435562 unspecified role unspecified role neutral component neutral component n/a 1435560 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 2b, 2d, 2e, 3a, 3b, 3c ZM447439 (aurora B kinase inhibitor), upon MG132 treatment (required to stop cell cycle and make the effect detectable). Interaction found during pro-metaphase, metaphase, anaphase and telophase. Interaction detected in the kinetochore pre-anaphase and in the spindle midzone post-anaphase. incenp-plk1 1435558 n/a 1435564 unspecified role unspecified role neutral component neutral component n/a 1435560 ancillary ancillary neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 4a Interaction found during all cell-cycle phases. The interaction greatly increases during anaphase, when it is located in the central spindle, and even more during telophase, when it locates to the midbody. birc5-cdca8-14 1435559 n/a 1435561 unspecified role unspecified role neutral component neutral component n/a 1435562 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2a Interaction detected during the metaphase.