DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Nature structural & molecular biology 2010 imex curation Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. DIP original experiment: DIP-218343X Nature structural & molecular biology 2010 IMEx Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Nature structural & molecular biology 2010 imex curation Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. DIP original experiment: DIP-218341X Nature structural & molecular biology 2010 IMEx Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Nature structural & molecular biology 2010 imex curation Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. DIP original experiment: DIP-218340X Nature structural & molecular biology 2010 IMEx Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Nature structural & molecular biology 2010 imex curation Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. DIP original experiment: DIP-218342X Nature structural & molecular biology 2010 IMEx Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Nature structural & molecular biology 2010 imex curation Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. DIP original experiment: DIP-218344X Nature structural & molecular biology 2010 IMEx Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Chemical-genomic dissection of the CTD code. Nature structural & molecular biology 2010 imex curation Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Tietjen JR., Zhang DW., Rodríguez-Molina JB., White BE., Akhtar MS., Heidemann M., Li X., Chapman RD., Shokat K., Keles S., Eick D., Ansari AZ. DIP original experiment: DIP-218339X Nature structural & molecular biology 2010 IMEx Chemical-genomic dissection of the CTD code. kin28_yeast Serine/threonine-protein kinase KIN28 D2330 YDL108W KIN28 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKVNMEYTKEKKVGEGTYAVVYLGCQHSTGRKIAIKEIKTSEFKDGLDMSAIREVKYLQEMQHPNVIELIDIFMAYDNLNLVLEFLPTDLEVVIKDKSILFTPADIKAWMLMTLRGVYHCHRNFILHRDLKPNNLLFSPDGQIKVADFGLARAIPAPHEILTSNVVTRWYRAPELLFGAKHYTSAIDIWSVGVIFAELMLRIPYLPGQNDVDQMEVTFRALGTPTDRDWPEVSSFMTYNKLQIYPPPSRDELRKRFIAASEYALDFMCGMLTMNPQKRWTAVQCLESDYFKELPPPSDPSSIKIRN 763A5720A1D9ACF3 763A5720A1D9ACF3 ctd CTD peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) YSPTSPSYSPTSPSYSPTSPSYSPTSPS rpb1_yeast DNA-directed RNA polymerase II subunit RPB1 RPO21 RPB220 RPB1 SUA8 YDL140C D2150 DNA-directed RNA polymerase III largest subunit RNA polymerase II subunit B220 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVGQQYSSAPLRTVKEVQFGLFSPEEVRAISVAKIRFPETMDETQTRAKIGGLNDPRLGSIDRNLKCQTCQEGMNECPGHFGHIDLAKPVFHVGFIAKIKKVCECVCMHCGKLLLDEHNELMRQALAIKDSKKRFAAIWTLCKTKMVCETDVPSEDDPTQLVSRGGCGNTQPTIRKDGLKLVGSWKKDRATGDADEPELRVLSTEEILNIFKHISVKDFTSLGFNEVFSRPEWMILTCLPVPPPPVRPSISFNESQRGEDDLTFKLADILKANISLETLEHNGAPHHAIEEAESLLQFHVATYMDNDIAGQPQALQKSGRPVKSIRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLELDQVGVPKSIAKTLTYPEVVTPYNIDRLTQLVRNGPNEHPGAKYVIRDSGDRIDLRYSKRAGDIQLQYGWKVERHIMDNDPVLFNRQPSLHKMSMMAHRVKVIPYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPCMGIVQDTLCGIRKLTLRDTFIELDQVLNMLYWVPDWDGVIPTPAIIKPKPLWSGKQILSVAIPNGIHLQRFDEGTTLLSPKDNGMLIIDGQIIFGVVEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLHNGFSTGIGDTIADGPTMREITETIAEAKKKVLDVTKEAQANLLTAKHGMTLRESFEDNVVRFLNEARDKAGRLAEVNLKDLNNVKQMVMAGSKGSFINIAQMSACVGQQSVEGKRIAFGFVDRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDNTTRNSLGNVIQFIYGEDGMDAAHIEKQSLDTIGGSDAAFEKRYRVDLLNTDHTLDPSLLESGSEILGDLKLQVLLDEEYKQLVKDRKFLREVFVDGEANWPLPVNIRRIIQNAQQTFHIDHTKPSDLTIKDIVLGVKDLQENLLVLRGKNEIIQNAQRDAVTLFCCLLRSRLATRRVLQEYRLTKQAFDWVLSNIEAQFLRSVVHPGEMVGVLAAQSIGEPATQMTLNTFHFAGVASKKVTSGVPRLKEILNVAKNMKTPSLTVYLEPGHAADQEQAKLIRSAIEHTTLKSVTIASEIYYDPDPRSTVIPEDEEIIQLHFSLLDEEAEQSFDQQSPWLLRLELDRAAMNDKDLTMGQVGERIKQTFKNDLFVIWSEDNDEKLIIRCRVVRPKSLDAETEAEEDHMLKKIENTMLENITLRGVENIERVVMMKYDRKVPSPTGEYVKEPEWVLETDGVNLSEVMTVPGIDPTRIYTNSFIDIMEVLGIEAGRAALYKEVYNVIASDGSYVNYRHMALLVDVMTTQGGLTSVTRHGFNRSNTGALMRCSFEETVEILFEAGASAELDDCRGVSENVILGQMAPIGTGAFDVMIDEESLVKYMPEQKITEIEDGQDGGVTPYSNESGLVNADLDVKDELMFSPLVDSGSNDAMAGGFTAYGGADYGEATSPFGAYGEAPTSPGFGVSSPGFSPTSPTYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPGYSPGSPAYSPKQDEQKHNENENSR A45C1360FF99F968 A45C1360FF99F968 bur1_yeast Serine/threonine-protein kinase BUR1 SGV1 BUR1 YPR161C P9584.8 Bypass UAS requirement protein 1 Suppressor of GPA1-Vall50 mutation protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDNGSPAVLPKTEFNKYKIGKVKSTPAIQRDAKTNLTYIKLRKRSSEKVYGCTVFQNHYREDEKLGQGTFGEVYKGIHLETQRQVAMKKIIVSVEKDLFPITAQREITILKRLNHKNIIKLIEMVYDHSPDITNAASSNLHKSFYMILPYMVADLSGVLHNPRINLEMCDIKNMMLQILEGLNYIHCAKFMHRDIKTANILIDHNGVLKLADFGLARLYYGCPPNLKYPGGAGSGAKYTSVVVTRWYRAPELVLGDKQYTTAVDIWGVGCVFAEFFEKKPILQGKTDIDQGHVIFKLLGTPTEEDWAVARYLPGAELTTTNYKPTLRERFGKYLSETGLDFLGQLLALDPYKRLTAMSAKHHPWFKEDPLPSEKITLPTEESHEADIKRYKEEMHQSLSQRVPTAPRGHIVEKGESPVVKNLGAIPRGPKKDDASFLPPSKNVLAKPPPSKIRELHQNPRPYHVNSGYAKTAIPPPAAPAGVNRYGPNNSSRNNRFSGNSTAPNNSRNPVNRFHPETNVSSKYNKVPLPLGPQSRYQGNSNESRYKNSPNDSRYHNPRYVNKPETNFNRQPQKYSRQESNAPINKNYNPSNGSRNMAGDHHQGSRPSHPQFPISPSQGQHQLTSKPIEKKNGSFKDERAKPDESKEFQNSDIADLY CC9034A4B10A510E CC9034A4B10A510E ctk1_yeast CTD kinase subunit alpha CTK1 CTD kinase subunit 1 CTD kinase 58 kDa subunit YKL139W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSYNNGNTYSKSYSRNNKRPLFGKRSPNPQSLARPPPPKRIRTDSGYQSNMDNISSHRVNSNDQPGHTKSRGNNNLSRYNDTSFQTSSRYQGSRYNNNNTSYENRPKSIKRDETKAEFLSHLPKGPKSVEKSRYNNSSNTSNDIKNGYHASKYYNHKGQEGRSVIAKKVPVSVLTQQRSTSVYLRIMQVGEGTYGKVYKAKNTNTEKLVALKKLRLQGEREGFPITSIREIKLLQSFDHPNVSTIKEIMVESQKTVYMIFEYADNDLSGLLLNKEVQISHSQCKHLFKQLLLGMEYLHDNKILHRDVKGSNILIDNQGNLKITDFGLARKMNSRADYTNRVITLWYRPPELLLGTTNYGTEVDMWGCGCLLVELFNKTAIFQGSNELEQIESIFKIMGTPTINSWPTLYDMPWFFMIMPQQTTKYVNNFSEKFKSVLPSSKCLQLAINLLCYDQTKRFSATEALQSDYFKEEPKPEPLVLDGLVSCHEYEVKLARKQKRPNILSTNTNNKGNGNSNNNNNNNNDDDDK 9862EB10FD476F6B 9862EB10FD476F6B ssn8_yeast RNA polymerase II holoenzyme cyclin-like subunit SSN8 SRB11 GIG3 YNL025C N2805 NUT9 UME3 Suppressor of RNA polymerase B 11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGSFWTSTQRHHWQYTKASLAKERQKLWLLECQLFPQGLNIVMDSKQNGIEQSITKNIPITHRDLHYDKDYNLRIYCYFLIMKLGRRLNIRQYALATAHIYLSRFLIKASVREINLYMLVTTCVYLACKVEECPQYIRTLVSEARTLWPEFIPPDPTKVTEFEFYLLEELESYLIVHHPYQSLKQIVQVLKQPPFQITLSSDDLQNCWSLINDSYINDVHLLYPPHIIAVACLFITISIHGKPTKGSSLASAASEAIRDPKNSSSPVQIAFNRFMAESLVDLEEVMDTIQEQITLYDHWDKYHEQWIKFLLHTLYLRPASAI 2BA16A3374CCF207 2BA16A3374CCF207 ctd4-kin28-1 2 8 enzyme target enzyme target substrate substrate unspecified role unspecified role pSer pSer opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 7 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation bur1-ctd-1 6 10 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 enzyme target enzyme target substrate substrate unspecified role unspecified role YSPTSPS-repeat domain Region 1556-1653 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pSer pSer opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation ctd4-ctk1-1 5 11 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 enzyme target enzyme target substrate substrate unspecified role unspecified role pSer pSer opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation ctd-kin28-1 3 7 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 enzyme target enzyme target substrate substrate unspecified role unspecified role YSPTSPS-repeat domain Region 1556-1653 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pSer pSer opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation ctd4-srb11-1 4 12 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 enzyme target enzyme target substrate substrate unspecified role unspecified role pSer pSer opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation bur1-ctd4-1 1 8 enzyme target enzyme target substrate substrate unspecified role unspecified role pSer pSer opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 10 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation