DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure (London, England : 1993) 2009 imex curation Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. DIP original experiment: DIP-132190X Structure (London, England : 1993) 2009 IMEx Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure (London, England : 1993) 2009 imex curation Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. DIP original experiment: DIP-132191X Structure (London, England : 1993) 2009 IMEx Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure (London, England : 1993) 2009 imex curation Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa tag visualisation tag visualisation Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. DIP original experiment: DIP-132193X Structure (London, England : 1993) 2009 IMEx Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure (London, England : 1993) 2009 imex curation Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Ganesan R., Eigenbrot C., Wu Y., Liang WC., Shia S., Lipari MT., Kirchhofer D. DIP original experiment: DIP-132192X Structure (London, England : 1993) 2009 IMEx Unraveling the allosteric mechanism of serine protease inhibition by an antibody. kqlr KQLR inhibitor peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KQLR hgf_human Hepatocyte growth factor HGF HPTA Scatter factor Hepatopoietin-A protein protein human Homo sapiens Human MWVTKLLPALLLQHVLLHLLLLPIAIPYAEGQRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS DIP protein P14210 original sequence version: 186 DIP protein P14210 original sequence version: 122 DIP protein P14210 original sequence version: 136 DIP protein P14210-1 original sequence version: 153 2D997938295ADD2F o43278-1 Kunitz-type protease inhibitor 1 UNQ223/PRO256 HAI-1B Hepatocyte growth factor activator inhibitor type 1 SPINT1 HAI1 protein protein human Homo sapiens Human MAPARTMARARLAPAGIPAVALWLLCTLGLQGTQAGPPPAPPGLPAGADCLNSFTAGVPGFVLDTNASVSNGATFLESPTVRRGWDCVRACCTTQNCNLALVELQPDRGEDAIAACFLINCLYEQNFVCKFAPREGFINYLTREVYRSYRQLRTQGFGGSGIPKAWAGIDLKVQPQEPLVLKDVENTDWRLLRGDTDVRVERKDPNQVELWGLKEGTYLFQLTVTSSDHPEDTANVTVTVLSTKQTEDYCLASNKVGRCRGSFPRWYYDPTEQICKSFVYGGCLGNKNNYLREEECILACRGVQGGPLRGSSGAQATFPQGPSMERRHPVCSGTCQPTQFRCSNGCCIDSFLECDDTPNCPDASDEAACEKYTSGFDELQRIHFPSDKGHCVDLPDTGLCKESIPRWYYNPFSEHCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREIPIPSTGSVEMAVAVFLVICIVVVVAILGYCFFKNQRKDFHGHHHHPPPTPASSTVSTTEDTEHLVYNHTTRPL DIP protein O43278-1 original sequence version: 102 hgfa_human Hepatocyte growth factor activator HGFAC protein protein human Homo sapiens Human MGRWAWVPSPWPPPGLGPFLLLLLLLLLLPRGFQPQPGGNRTESPEPNATATPAIPTILVTSVTSETPATSAPEAEGPQSGGLPPPPRAVPSSSSPQAQALTEDGRPCRFPFRYGGRMLHACTSEGSAHRKWCATTHNYDRDRAWGYCVEATPPPGGPAALDPCASGPCLNGGSCSNTQDPQSYHCSCPRAFTGKDCGTEKCFDETRYEYLEGGDRWARVRQGHVEQCECFGGRTWCEGTRHTACLSSPCLNGGTCHLIVATGTTVCACPPGFAGRLCNIEPDERCFLGNGTGYRGVASTSASGLSCLAWNSDLLYQELHVDSVGAAALLGLGPHAYCRNPDNDERPWCYVVKDSALSWEYCRLEACESLTRVQLSPDLLATLPEPASPGRQACGRRHKKRTFLRPRIIGGSSSLPGSHPWLAAIYIGDSFCAGSLVHTCWVVSAAHCFSHSPPRDSVSVVLGQHFFNRTTDVTQTFGIEKYIPYTLYSVFNPSDHDLVLIRLKKKGDRCATRSQFVQPICLPEPGSTFPAGHKCQIAGWGHLDENVSGYSSSLREALVPLVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLACEKNGVAYLYGIISWGDGCGRLHKPGVYTRVANYVDWINDRIRPPRRLVAPS DIP protein Q04756 original sequence version: 105 2CF72F1E1B862ED7 hgfac-kqlr-1 1 5 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role Region 373-655 Region 373-655 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction hgfac-spint1-2 3 7 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 8 unspecified role unspecified role bait bait Region 373-655 Region 373-655 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction hgfac-kqlr-2 2 5 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role Region 373-655 Region 373-655 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction hgf-hgfac-1 4 8 enzyme enzyme unspecified role unspecified role Region 373-655 Region 373-655 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 6 enzyme target enzyme target substrate substrate unspecified role unspecified role 125I-label 125I-label 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster cleavage cleavage reaction cleavage