mpidb The Microbial Protein Interaction Database Oligomeric sensor kinase DcuS in the membrane of Escherichia coli and in proteoliposomes: chemical cross-linking and FRET spectroscopy. Oligomeric sensor kinase DcuS in the membrane of Escherichia coli and in proteoliposomes: chemical cross-linking and FRET spectroscopy. J. Bacteriol. (0021-9193) 2010 imex curation Scheu P.D., Liao Y.F., Bauer J., Kneuper H., Basché T., Unden G., Erker W. Only protein-protein interactions in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Scheu P.D., Liao Y.F., Bauer J., Kneuper H., Basché T., Unden G., Erker W. Accepted 2013-FEB-19 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation Oligomeric sensor kinase DcuS in the membrane of Escherichia coli and in proteoliposomes: chemical cross-linking and FRET spectroscopy. Oligomeric sensor kinase DcuS in the membrane of Escherichia coli and in proteoliposomes: chemical cross-linking and FRET spectroscopy. J. Bacteriol. (0021-9193) 2010 imex curation Scheu P.D., Liao Y.F., Bauer J., Kneuper H., Basché T., Unden G., Erker W. Only protein-protein interactions ecolx Escherichia coli comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Scheu P.D., Liao Y.F., Bauer J., Kneuper H., Basché T., Unden G., Erker W. Accepted 2013-FEB-19 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation Oligomeric sensor kinase DcuS in the membrane of Escherichia coli and in proteoliposomes: chemical cross-linking and FRET spectroscopy. Oligomeric sensor kinase DcuS in the membrane of Escherichia coli and in proteoliposomes: chemical cross-linking and FRET spectroscopy. J. Bacteriol. (0021-9193) 2010 imex curation Scheu P.D., Liao Y.F., Bauer J., Kneuper H., Basché T., Unden G., Erker W. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Scheu P.D., Liao Y.F., Bauer J., Kneuper H., Basché T., Unden G., Erker W. Accepted 2013-FEB-19 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation Oligomeric sensor kinase DcuS in the membrane of Escherichia coli and in proteoliposomes: chemical cross-linking and FRET spectroscopy. Oligomeric sensor kinase DcuS in the membrane of Escherichia coli and in proteoliposomes: chemical cross-linking and FRET spectroscopy. J. Bacteriol. (0021-9193) 2010 imex curation Scheu P.D., Liao Y.F., Bauer J., Kneuper H., Basché T., Unden G., Erker W. Only protein-protein interactions ecoli Escherichia coli (strain K12) fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Scheu P.D., Liao Y.F., Bauer J., Kneuper H., Basché T., Unden G., Erker W. Accepted 2013-FEB-19 by ORCHARD J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation dcus_ecoli Sensor histidine kinase DcuS yjdH dcuS b4125 JW4086 Fumarate sensor protein protein ecoli Escherichia coli (strain K12) MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQIGVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRLDGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR 653C369344CBA238 dcus-2 2 5 unspecified role unspecified role neutral component neutral component region P0AEC8-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0AEC8:p.[Cys199Ser;Cys471Ser] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:55:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys199ser,cys471ser' Tue Jun 30 19:42:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys199Ser;Cys471Ser]' P0AEC8:p.Cys471Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:01:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys471ser' Tue Jun 30 16:28:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys471Ser' physical association physical association Figure 1 dcus-dcus-1 3 5 unspecified role unspecified role self self region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role self self region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2 dcus-1 3 5 unspecified role unspecified role neutral component neutral component region P0AEC8-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 1 dcus-dcus-3 1 5 unspecified role unspecified role fluorescence donor fluorescence donor region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region alexa 594 label alexa 594 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Figure 4A dcus-dcus-5 4 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region alexa 594 label alexa 594 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role fluorescence donor fluorescence donor region alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Figure 5 dcus-dcus-6 4 5 unspecified role unspecified role fluorescence donor fluorescence donor region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Figure 6