UniProt UniProt http://www.uniprot.org Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. J. Biol. Chem. (0021-9258) 2007 mimix curation Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de 20-Jun-2012: Contacted by JYOTI. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de Accepted 2012-MAY-14 by ORCHARD 20-Jun-2012: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 mimix curation Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. J. Biol. Chem. (0021-9258) 2007 mimix curation Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de 20-Jun-2012: Contacted by JYOTI. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de Accepted 2012-MAY-14 by ORCHARD 20-Jun-2012: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 mimix curation Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. J. Biol. Chem. (0021-9258) 2007 mimix curation Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de 20-Jun-2012: Contacted by JYOTI. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de Accepted 2012-MAY-14 by ORCHARD 20-Jun-2012: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 mimix curation Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. J. Biol. Chem. (0021-9258) 2007 mimix curation Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de 20-Jun-2012: Contacted by JYOTI. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de Accepted 2012-MAY-14 by ORCHARD 20-Jun-2012: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 mimix curation Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. J. Biol. Chem. (0021-9258) 2007 mimix curation Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de 20-Jun-2012: Contacted by JYOTI. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de Accepted 2012-MAY-14 by ORCHARD J. Biol. Chem. (0021-9258) 2007 mimix curation Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. J. Biol. Chem. (0021-9258) 2007 mimix curation Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de 20-Jun-2012: Contacted by JYOTI. rat-brain Rattus norvegicus brain brain Brain [cerebrum] confocal microscopy confocal microscopy immunostaining immunostaining Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de Accepted 2012-MAY-14 by ORCHARD 20-Jun-2012: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 mimix curation gpm6a_rat Neuronal membrane glycoprotein M6-a Gpm6a m6a protein protein rat Rattus norvegicus (Rat) Rat MEENMEEGQTQKGCFECCIKCLGGIPYASLIATILLYAGVALFCGCGHEALSGTVNILQTYFEMARTAGDTLDVFTMIDIFKYVIYGIAAAFFVYGILLMVEGFFTTGAIKDLYGDFKITTCGRCVSAWFIMLTYLFMLAWLGVTAFTSLPVYMYFNVWTICRNTTLVEGANLCLDLRQFGIVTIGEEKKICTVSENFLRMCESTELNMTFHLFIVALAGAGAAVIAMVHYLMVLSANWAYVKDACRMQKYEDIKSKEEQELHDIHSTRSKERLNAYT D2EDAF98C0E8715D oprm_rat Mu-type opioid receptor Oprm1 Ror-b Opioid receptor B protein protein rat Rattus norvegicus (Rat) Rat MDSSTGPGNTSDCSDPLAQASCSPAPGSWLNLSHVDGNQSDPCGLNRTGLGGNDSLCPQTGSPSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTIEQQNSTRVRQNTREHPSTANTVDRTNHQLENLEAETAPLP 9C916DE7C1C33743 oprm1-gpm6a 5 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association no figure oprm1-gpm6a-1 1 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 1A oprm1-gpm6a-2 4 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 1B gpm6a-oprm1 4 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 1B gpm6a-oprm1-1 3 8 unspecified role unspecified role fluorescence donor fluorescence donor 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept proximity proximity close-contact colocalization neighbourhood interaction 1C, 2B and C oprm1-gpm6a-3 2 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey direct interaction direct interaction 3 gpm6a-oprm1-2 6 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 6D and 7 Monensin and DAMGO treatment enhance colocalization in cytoplasmuc vesicles.