IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element. Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element. J. Biol. Chem. (0021-9258) 1995 imex curation Johnson EM., Chen PL., Krachmarov CP., Barr SM., Kanovsky M., Ma ZW., Lee WH. Cancer - Interactions investigated in the context of cancer johnson@msvax.mssm.edu 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Johnson EM., Chen PL., Krachmarov CP., Barr SM., Kanovsky M., Ma ZW., Lee WH. Cancer - Interactions investigated in the context of cancer johnson@msvax.mssm.edu Accepted 2010-FEB-5 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 1995 orchard Only protein-protein interactions imex curation Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element. Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element. J. Biol. Chem. (0021-9258) 1995 imex curation Johnson EM., Chen PL., Krachmarov CP., Barr SM., Kanovsky M., Ma ZW., Lee WH. Cancer - Interactions investigated in the context of cancer johnson@msvax.mssm.edu 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast anti bait coip anti bait coimmunoprecipitation anti bait coip monoclonal western monoclonal antibody western blot monoclonal western Johnson EM., Chen PL., Krachmarov CP., Barr SM., Kanovsky M., Ma ZW., Lee WH. Cancer - Interactions investigated in the context of cancer Extracts were prepared from cells, pulsed in log phase with [35S]Met. Autoradiography was also used to detect the presence of the protein. johnson@msvax.mssm.edu Accepted 2010-FEB-5 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 1995 orchard Only protein-protein interactions imex curation Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element. Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element. J. Biol. Chem. (0021-9258) 1995 imex curation Johnson EM., Chen PL., Krachmarov CP., Barr SM., Kanovsky M., Ma ZW., Lee WH. Cancer - Interactions investigated in the context of cancer johnson@msvax.mssm.edu 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Johnson EM., Chen PL., Krachmarov CP., Barr SM., Kanovsky M., Ma ZW., Lee WH. Cancer - Interactions investigated in the context of cancer johnson@msvax.mssm.edu Accepted 2010-FEB-5 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 1995 orchard Only protein-protein interactions imex curation Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element. Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element. J. Biol. Chem. (0021-9258) 1995 imex curation Johnson EM., Chen PL., Krachmarov CP., Barr SM., Kanovsky M., Ma ZW., Lee WH. Cancer - Interactions investigated in the context of cancer johnson@msvax.mssm.edu 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Johnson EM., Chen PL., Krachmarov CP., Barr SM., Kanovsky M., Ma ZW., Lee WH. Cancer - Interactions investigated in the context of cancer johnson@msvax.mssm.edu Accepted 2010-FEB-5 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 1995 orchard Only protein-protein interactions imex curation Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element. Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element. J. Biol. Chem. (0021-9258) 1995 imex curation Johnson EM., Chen PL., Krachmarov CP., Barr SM., Kanovsky M., Ma ZW., Lee WH. Cancer - Interactions investigated in the context of cancer johnson@msvax.mssm.edu 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Johnson EM., Chen PL., Krachmarov CP., Barr SM., Kanovsky M., Ma ZW., Lee WH. Cancer - Interactions investigated in the context of cancer johnson@msvax.mssm.edu Accepted 2010-FEB-5 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 1995 orchard Only protein-protein interactions imex curation pura_human Transcriptional activator protein Pur-alpha Purine-rich single-stranded DNA-binding protein alpha PURA PUR1 protein protein human Homo sapiens Human MADRDSGSEQGGAALGSGGSLGHPGSGSGSGGGGGGGGGGGGSGGGGGGAPGGLQHETQELASKRVDIQNKRFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDLAQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGVEEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPYKVWAKFGHTFCKYSEEMKKIQEKQREKRAACEQLHQQQQQQQEETAAATLLLQGEEEGEED 797968504D01B356 rb_cerae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt Similar to P06400 rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK DIP protein P06400 original sequence version: 102 DIP protein P06400 original sequence version: 195 DIP protein P06400 original sequence version: 204 DIP protein P06400 original sequence version: 126 DIP protein P06400 original sequence version: 151 DIP protein P06400 original sequence version: 153 DIP protein P06400 original sequence version: 181 DIP protein P06400 original sequence version: 184 DIP protein P06400 original sequence version: 213 DIP protein P06400 original sequence version: 208 DIP protein P06400 original sequence version: 174 DIP protein P06400 original sequence version: 212 DIP protein P06400 original sequence version: 179 DIP protein P06400 original sequence version: 146 C8E746111E19CC32 pura_cerae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET 3E37D4BAFC5D59BD rb-pur 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey human-wr2e3 Homo sapiens WR2E3 cells wr2e3 WR2E3 cells physical association physical association 1, 6 shows that binding of Rb to GST-Purα and GST-T is approximately equal. Phosphorylation of RB1 rb-sv40t 1 8 unspecified role unspecified role prey prey human-wr2e3 Homo sapiens WR2E3 cells wr2e3 WR2E3 cells 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1 shows that binding of Rb to GST-Purα and GST-T is approximately equal. Positive control rb-pur-2 2 9 unspecified role unspecified role bait bait methionine 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey methionine 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2 Immunoprecipitation of Purα�Rb Complexes from Lysates of CV-1� pur-rb 3 8 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 An Anti-Rb Monoclonal Antibody Immunoprecipitates a Post-synthetically Modified Form of Purα from Lysates of Transfected CV-1 Cells P06400; Q00577 transfection; rb-pur-3 1 10 inhibitor inhibitor unspecified role unspecified role lys lys tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-wr2e3 Homo sapiens WR2E3 cells wr2e3 WR2E3 cells physical association physical association 7 Purα Binds the COOH-terminal Portion of Rb, and This Binding Is Inhibited by a Peptide Containing the T-antigen Rb-binding Site rb-pur-4 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-wr2e3 Homo sapiens WR2E3 cells wr2e3 WR2E3 cells physical association physical association 7 Purα Binds the COOH-terminal Portion of Rb. pur-rb-1 4 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait physical association physical association 3 pur-rb-2 5 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait physical association physical association 3