IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. J. Biol. Chem. (0021-9258) 2006 imex curation Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-endothelium Homo sapiens endothelail cells/endothelium endothelium Endothelial cells [endothelium] confocal microscopy confocal microscopy predetermined predetermined participant predetermined Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl Accepted 2006-SEP-13 by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. J. Biol. Chem. (0021-9258) 2006 imex curation Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa predetermined predetermined participant predetermined Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. J. Biol. Chem. (0021-9258) 2006 imex curation Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells facs fluorescence-activated cell sorting FACS Flow cytometry facs immunostaining immunostaining Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. J. Biol. Chem. (0021-9258) 2006 imex curation Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] facs fluorescence-activated cell sorting FACS Flow cytometry facs immunostaining immunostaining Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation pai1_human Plasminogen activator inhibitor 1 Endothelial plasminogen activator inhibitor SERPINE1 PAI1 PLANH1 Serpin E1 protein protein human Homo sapiens Human MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP A2E181ED28DD6082 a1ag1_human Alpha-1-acid glycoprotein 1 Orosomucoid-1 ORM1 AGP1 protein protein human Homo sapiens Human MALSWVLTVLSLLPLLEAQIPLCANLVPVPITNATLDQITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQDQCIYNTTYLNVQRENGTISRYVGGQEHFAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES 63292233AD6EAD8B agp1-pai1 1 5 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Figure 4 A and Figure 5 A AGP preferentially interacted with the active form of PAI1 Binding of active PAI-1 to the AGP expressed on endothelial cells. The authors refer to protein construct PAI-14-1B but this is not descibed within the text or cited references. agp1-pai1-1 2 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component physical association physical association Figure 4 E AGP preferentially interacted with the active form of PAI1 E. shows interaction of different PAI-1 forms with AGP analyzed by a solid phase binding assay. agp1-pai1-2 4 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component P05121:p.[Asn173His;Lys177Thr;Gln342Leu;Met377Ile] mutation mutation certain certain sequence position certain certain certain sequence position certain K T certain certain sequence position certain certain certain sequence position certain N H certain certain sequence position certain certain certain sequence position certain Q L certain certain sequence position certain certain certain sequence position certain M I Wed May 02 16:27:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn173his,lys177thr,gln342leu,met377ile' Tue Jun 30 18:25:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn173His;Lys177Thr;Gln342Leu;Met377Ile]' colocalization colocalization Figure 3 The authors state that a mutant form of P05121 was used in this study. Binding of PAI-1 to AGP on cell membranes. agp1-pai1-3 3 5 unspecified role unspecified role neutral component neutral component P05121:p.[Asn173His;Lys177Thr;Gln342Leu;Met377Ile] mutation mutation certain certain sequence position certain certain certain sequence position certain K T certain certain sequence position certain certain certain sequence position certain N H certain certain sequence position certain certain certain sequence position certain Q L certain certain sequence position certain certain certain sequence position certain M I Wed May 02 16:54:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn173his,lys177thr,gln342leu,met377ile' Tue Jun 30 17:06:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn173His;Lys177Thr;Gln342Leu;Met377Ile]' 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 3 The authors state that a mutant form of P05121 was used in this study. Binding of PAI-1 to AGP on cell membranes.