IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ stewart-2010-5 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ. kathryn.moore@nyumc.org Nature immunology 2010 imex curation Only protein-protein interactions stewart-2010-3 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ. kathryn.moore@nyumc.org Nature immunology 2010 imex curation Only protein-protein interactions stewart-2010-4 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ. kathryn.moore@nyumc.org Nature immunology 2010 imex curation Only protein-protein interactions stewart-2010-1 human-thp-1 Homo sapiens acute monocytic leukemia cells thp-1 Human acute monocytic leukemia cell line Human acute monocytic leukemia established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia (AML) at relapse in 1978. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ. kathryn.moore@nyumc.org Nature immunology 2010 imex curation Only protein-protein interactions stewart-2010-2 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ. kathryn.moore@nyumc.org Nature immunology 2010 imex curation Only protein-protein interactions stewart-2010-6 human-c32 C32 melanoma cell line, amelanotic (colourless) Human c32 C32 melanoma cell line, amelanotic (colourless) https://www.lgcstandards-atcc.org/products/all/CRL-1585 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ., Stewart CR., Stuart LM., Wilkinson K., van Gils JM., Deng J., Halle A., Rayner KJ., Boyer L., Zhong R., Frazier WA., Lacy-Hulbert A., El Khoury J., Golenbock DT., Moore KJ. kathryn.moore@nyumc.org Nature immunology 2010 imex curation Only protein-protein interactions paxi_human Paxillin PXN protein protein human Homo sapiens Human MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC cd36_human Platelet glycoprotein 4 GP3B GP4 Platelet glycoprotein IV Glycoprotein IIIb Leukocyte differentiation antigen CD36 PAS IV PAS-4 Platelet collagen receptor Fatty acid translocase Thrombospondin receptor CD36 protein protein human Homo sapiens Human MGCDRNCGLIAGAVIGAVLAVFGGILMPVGDLLIQKTIKKQVVLEEGTIAFKNWVKTGTEVYRQFWIFDVQNPQEVMMNSSNIQVKQRGPYTYRVRFLAKENVTQDAEDNTVSFLQPNGAIFEPSLSVGTEADNFTVLNLAVAAASHIYQNQFVQMILNSLINKSKSSMFQVRTLRELLWGYRDPFLSLVPYPVTTTVGLFYPYNNTADGVYKVFNGKDNISKVAIIDTYKGKRNLSYWESHCDMINGTDAASFPPFVEKSQVLQFFSSDICRSIYAVFESDVNLKGIPVYRFVLPSKAFASPVENPDNYCFCTEKIISKNCTSYGVLDISKCKEGRPVYISLPHFLYASPDVSEPIDGLNPNEEEHRTYLDIEPITGFTLQFAKRLQVNLLVKPSEKIQVLKNLKRNYIVPILWLNETGTIGDEKANMFRSQVTGKINLLGLIEMILLSVGVVMFVAFMISYCACRSKTIK fak1_human Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase Protein phosphatase 1 regulatory subunit 71 p125FAK protein protein human Homo sapiens Human MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH lyn_human Tyrosine-protein kinase Lyn JTK8 Lck/Yes-related novel protein tyrosine kinase V-yes-1 Yamaguchi sarcoma viral related oncogene homolog p53Lyn p56Lyn LYN protein protein human Homo sapiens Human MGCIKSKGKDSLSDDGVDLKTQPVRNTERTIYVRDPTSNKQQRPVPESQLLPGQRFQTKDPEEQGDIVVALYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLLTKKEGFIPSNYVAKLNTLETEEWFFKDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDFDPVHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQADGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKRLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTREEPIYIITEYMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMTALSQGYRMPRVENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP cd36_mouse Platelet glycoprotein 4 Cd36 Platelet glycoprotein IV PAS IV PAS-4 Glycoprotein IIIb protein protein mouse Mus musculus Mouse MGCDRNCGLIAGAVIGAVLAVFGGILMPVGDMLIEKTIKREVVLEEGTTAFKNWVKTGTTVYRQFWIFDVQNPDDVAKNSSKIKVKQRGPYTYRVRYLAKENITQDPEDHTVSFVQPNGAIFEPSLSVGTEDDNFTVLNLAVAAAPHIYQNSFVQVVLNSLIKKSKSSMFQTRSLKELLWGYKDPFLSLVPYPISTTVGVFYPYNDTVDGVYKVFNGKDNISKVAIIESYKGKRNLSYWPSYCDMINGTDAASFPPFVEKSRTLRFFSSDICRSIYAVFGSEIDLKGIPVYRFVLPANAFASPLQNPDNHCFCTEKVISNNCTSYGVLDIGKCKEGKPVYISLPHFLHASPDVSEPIEGLHPNEDEHRTYLDVEPITGFTLQFAKRLQVNILVKPARKIEALKNLKRPYIVPILWLNETGTIGDEKAEMFKTQVTGKIKLLGMVEMALLGIGVVMFVAFMISYCACKSKNGK mint Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport tlr4_human Toll-like receptor 4 TLR4 hToll protein protein human Homo sapiens Human MMSASRLAGTLIPAMAFLSCVRPESWEPCVEVVPNITYQCMELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYSFFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFSGLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI tlr6_human Toll-like receptor 6 TLR6 protein protein human Homo sapiens Human MTKDKEPIVKSFHFVCLMIIIVGTRIQFSDGNEFAVDKSKRGLIHVPKDLPLKTKVLDMSQNYIAELQVSDMSFLSELTVLRLSHNRIQLLDLSVFKFNQDLEYLDLSHNQLQKISCHPIVSFRHLDLSFNDFKALPICKEFGNLSQLNFLGLSAMKLQKLDLLPIAHLHLSYILLDLRNYYIKENETESLQILNAKTLHLVFHPTSLFAIQVNISVNTLGCLQLTNIKLNDDNCQVFIKFLSELTRGSTLLNFTLNHIETTWKCLVRVFQFLWPKPVEYLNIYNLTIIESIREEDFTYSKTTLKALTIEHITNQVFLFSQTALYTVFSEMNIMMLTISDTPFIHMLCPHAPSTFKFLNFTQNVFTDSIFEKCSTLVKLETLILQKNGLKDLFKVGLMTKDMPSLEILDVSWNSLESGRHKENCTWVESIVVLNLSSNMLTDSVFRCLPPRIKVLDLHSNKIKSVPKQVVKLEALQELNVAFNSLTDLPGCGSFSSLSVLIIDHNSVSHPSADFFQSCQKMRSIKAGDNPFQCTCELREFVKNIDQVSSEVLEGWPDSYKCDYPESYRGSPLKDFHMSELSCNITLLIVTIGATMLVLAVTVTSLCIYLDLPWYLRMVCQWTQTRRRARNIPLEELQRNLQFHAFISYSEHDSAWVKSELVPYLEKEDIQICLHERNFVPGKSIVENIINCIEKSYKSIFVLSPNFVQSEWCHYELYFAHHNLFHEGSNNLILILLEPIPQNSIPNKYHKLKALMTQRTYLQWPKEKSKRGLFWANIRAAFNMKLTLVTENNDVKS lyn-cd36-1 2957544 N/A 2957550 unspecified role unspecified role prey prey possible_phosphres nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] N/A 2957551 unspecified role unspecified role bait bait c-terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2957552 unspecified role unspecified role prey prey possible_phosres nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] association association false false false Supp figure 4A Results from blasting the CD36 peptide sequence used for Pull down gives 100% homology with Human CD36, but only ~90% with the Mouse protein. A protein of approximately 60 kDa was also pulled down- and shown to be Lyn.. cd36-lyn-1 2957544 N/A 2957551 competitor competitor competitor unspecified role unspecified role c-terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false MISY_fragment required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2957551 unspecified role unspecified role bait bait c-terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2957553 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false 6C-bottom-i Results from blasting the CD36 peptide sequence used for Pull down gives 100% homology with Human CD36, but only ~90% with the Mouse protein. cd36-lyn-2 2957544 N/A 2957551 unspecified role unspecified role bait bait c-terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2957553 unspecified role unspecified role prey prey cell lysate cell lysate human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false Fig 6C -bottom figure Results from blasting the CD36 peptide sequence used for Pull down gives 100% homology with Human CD36, but only ~90% with the Mouse protein. cd36-tlr4-2 2957545 N/A 2957554 immunocytochemistry immunocytochemistry unspecified role unspecified role ancillary ancillary Q08857:p.Tyr463Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 2957555 unspecified role unspecified role bait bait c_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 2957556 unspecified role unspecified role prey prey association association false false false 6B oxidized LDL tlr4-tlr6-1 2957545 N/A 2957555 unspecified role unspecified role bait bait c_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 2957556 unspecified role unspecified role prey prey physical association physical association false false false 6B lane 2 very weak interaction lyn-cd36-3 2957546 N/A 2957554 unspecified role unspecified role prey prey N/A 2957553 unspecified role unspecified role bait bait physical association physical association false false false 6C-top lyn-cd36-2 2957546 N/A 2957554 unspecified role unspecified role bait bait N/A 2957550 unspecified role unspecified role prey prey N/A 2957553 unspecified role unspecified role prey prey association association false false false Supp figure 4B 4234-1 2957547 N/A 2957556 unspecified role unspecified role prey prey N/A 2957555 unspecified role unspecified role prey prey N/A 2957551 unspecified role unspecified role bait bait association association false false false 5 A/B, 6E oxidized LDL amyloid-beta peptide, amino acids 1–42 The authors state that "treatment with the Lyn kinase inhibitor PP1...blocked the physical association of CD36 with TLR4 and TLR6 in oxLDL-treated monocytes". cd36-tlr4-1 2957548 N/A 2957554 immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component N/A 2957556 unspecified role unspecified role neutral component neutral component c_terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 2957555 unspecified role unspecified role neutral component neutral component c_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false 5C oxidized LDL Minimal colocalization of CD36, TLR4 and TLR6 was seen in resting cells, but upon treatment with oxLDL, CD36, TLR4 and TLR6 colocalized predominantly in intracellular compartments. cd36-lyn-3 2957549 N/A 2957550 unspecified role unspecified role prey prey N/A 2957551 unspecified role unspecified role bait bait N/A 2957553 unspecified role unspecified role prey prey association association false false false Supp figure 4Bi