DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kato-2008-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Kato M., Wynn RM., Chuang JL., Tso SC., Machius M., Li J., Chuang DT. DIP original experiment: DIP-132184X david.chuang@utsouthwetstern.edu Structure (London, England : 1993) 2008 IMEx Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops. Kato M., Wynn RM., Chuang JL., Tso SC., Machius M., Li J., Chuang DT. Only protein-protein interactions imex curation kato-2008-4 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc experimental particp experimental participant identification experimental particp Kato M., Wynn RM., Chuang JL., Tso SC., Machius M., Li J., Chuang DT. david.chuang@utsouthwetstern.edu Structure (London, England : 1993) 2008 Only protein-protein interactions imex curation kato-2008-3 in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase experimental particp experimental participant identification experimental particp Kato M., Wynn RM., Chuang JL., Tso SC., Machius M., Li J., Chuang DT. david.chuang@utsouthwetstern.edu Structure (London, England : 1993) 2008 Only protein-protein interactions imex curation p11177-1 Isoform 1 of Pyruvate dehydrogenase E1 component subunit beta, mitochondrial PDHB PHE1B protein protein human Homo sapiens Human MAAVSGLVRRPLREVSGLLKRRFHWTAPAALQVTVRDAINQGMDEELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSGGLQPVPIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVVLENELMYGVPFEFPPEAQSKDFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKKTLNI p08559-1 Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial PDHE1-A type I PDHA1 PHE1A protein protein human Homo sapiens Human MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS pdk4_human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial PDK4 PDHK4 Pyruvate dehydrogenase kinase isoform 4 protein protein human Homo sapiens Human MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM pdha1-pdhb-3 3181682 N/A 3181685 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component 31-359 binding region 31-359 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3181686 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component P08559-1:p.[Ser232Ala;Ser300Ala] S271A,S203A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P08559-1:p.Tyr118Phe Y89F mutation mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false 30-390 binding region 30-390 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 1,Fig 3,Table 2 pdha1-pdhb-4 3181682 N/A 3181685 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component 31-359 binding region 31-359 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3181686 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component P08559-1:p.[Ser232Ala;Ser300Ala] S271A,S203A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 30-390 binding region 30-390 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false pSer293 pho S1 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 3,Table 2 pdha1-pdhb-5 3181683 N/A 3181685 unspecified role unspecified role neutral component neutral component 128-265 binding region 128-265 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false xpress tag xpress tag xpress tag xpress tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3181686 unspecified role unspecified role neutral component neutral component P08559-1:p.[Ser232Ala;Ser300Ala] S271A,S203A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 30-390 binding region 30-390 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 6 pdha1-pdhb-6 3181683 N/A 3181685 unspecified role unspecified role neutral component neutral component 128-265 binding region 128-265 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false xpress tag xpress tag xpress tag xpress tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3181686 unspecified role unspecified role neutral component neutral component P08559-1:p.[Ser232Ala;Ser300Ala] S271A,S203A mutation mutation substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 30-390 binding region 30-390 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false opser opser opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 6 pdha1-pdk4-1 3181684 N/A 3181687 predetermined predetermined participant predetermined enzyme enzyme unspecified role unspecified role purified purified 10-411 binding region 10-411 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false SUMO Tag SUMO Tag sumo tag sumo tag SUMO tag sumo tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3181686 predetermined predetermined participant predetermined enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified 30-390 binding region 30-390 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 32p radiolabel 32p radiolabel 32p radiolabel 32p radiolabel P32 32P 32P P32 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P08559-1:p.[Ser232Ala;Ser300Ala] S323A,S300A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P08559-1:p.[Ser232Ala;Ser293Ala] S232A,S293A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphotransfer phosphotransfer reaction phosphotransfer false false false Fig 2