IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hinek-2006-1 Lysosomal sialidase (neuraminidase-1) is targeted to the cell surface in a multiprotein complex that facilitates elastic fiber assembly. human-cls Homo sapiens fibroblast cell line (GM03318) cls Human fibroblast obtained from patients with Coffin-Lowry Syndrome (CLS). GM03318 (NIGMS Human Genetic Cell Repository) Catalog ID GM03318 (NIGMS Human Genetic Cell Repository) affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Hinek A., Pshezhetsky AV., von Itzstein M., Starcher B. BioCreative - Critical Assessment of Information Extraction systems in Biology Multi protein complex was isolated by elastin fixed to Affi-gel 10 using affinity chromatography. alek.hinek@sickkids.ca J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation hinek-2006-2 Lysosomal sialidase (neuraminidase-1) is targeted to the cell surface in a multiprotein complex that facilitates elastic fiber assembly. human-aorta Homo sapiens aorta confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Hinek A., Pshezhetsky AV., von Itzstein M., Starcher B. BioCreative - Critical Assessment of Information Extraction systems in Biology Human aortic smooth muscle cells were used in this study. Multi protein complex was isolated by elastin fixed to Affi-gel 10 using affinity chromatography. alek.hinek@sickkids.ca J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation hinek-2006-3 Lysosomal sialidase (neuraminidase-1) is targeted to the cell surface in a multiprotein complex that facilitates elastic fiber assembly. human-aorta Homo sapiens aorta electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy antibody detection identification by antibody antibody detection Hinek A., Pshezhetsky AV., von Itzstein M., Starcher B. BioCreative - Critical Assessment of Information Extraction systems in Biology Human aortic smooth muscle cells were used in this study. Multi protein complex was isolated by elastin fixed to Affi-gel 10 using affinity chromatography. alek.hinek@sickkids.ca J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation neur1_human Sialidase-1 Lysosomal sialidase N-acetyl-alpha-neuraminidase 1 Acetylneuraminyl hydrolase G9 sialidase NEU1 NANH protein protein human Homo sapiens Human MTGERPSTALPDRRWGPRILGFWGGCRVWVFAAIFLLLSLAASWSKAENDFGLVQPLVTMEQLLWVSGRQIGSVDTFRIPLITATPRGTLLAFAEARKMSSSDEGAKFIALRRSMDQGSTWSPTAFIVNDGDVPDGLNLGAVVSDVETGVVFLFYSLCAHKAGCQVASTMLVWSKDDGVSWSTPRNLSLDIGTEVFAPGPGSGIQKQREPRKGRLIVCGHGTLERDGVFCLLSDDHGASWRYGSGVSGIPYGQPKQENDFNPDECQPYELPDGSVVINARNQNNYHCHCRIVLRSYDACDTLRPRDVTFDPELVDPVVAAGAVVTSSGIVFFSNPAHPEFRVNLTLRWSFSNGTSWRKETVQLWPGPSGYSSLATLEGSMDGEEQAPQLYVLYEKGRNHYTESISVAKISVYGTL ppgb_human Lysosomal protective protein Cathepsin A Carboxypeptidase C Protective protein for beta-galactosidase CTSA PPGB Carboxypeptidase L Protective protein cathepsin A protein protein human Homo sapiens Human MIRAAPPPLFLLLLLLLLLVSWASRGEAAPDQDEIQRLPGLAKQPSFRQYSGYLKGSGSKHLHYWFVESQKDPENSPVVLWLNGGPGCSSLDGLLTEHGPFLVQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSDDKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVTNLQEVARIVGNSGLNIYNLYAPCAGGVPSHFRYEKDTVVVQDLGNIFTRLPLKRMWHQALLRSGDKVRMDPPCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAAFTMFSRFLNKQPY eln_bovin Elastin Tropoelastin ELN protein protein bovin Bos taurus (Bovine) Bovine MRSLTAAARRPEVLLLLLCILQPSQPGGVPGAVPGGVPGGVFFPGAGLGGLGVGGLGPGVKPAKPGVGGLVGPGLGAEGSALPGAFPGGFFGAGGGAAGAAAAYKAAAKAGAAGLGVGGIGGVGGLGVSTGAVVPQLGAGVGAGVKPGKVPGVGLPGVYPGGVLPGAGARFPGIGVLPGVPTGAGVKPKAQVGAGAFAGIPGVGPFGGQQPGLPLGYPIKAPKLPAGYGLPYKTGKLPYGFGPGGVAGSAGKAGYPTGTGVGPQAAAAAAKAAAKLGAGGAGVLPGVGVGGPGIPGAPGAIPGIGGIAGVGAPDAAAAAAAAAKAAKFGAAGGLPGVGVPGVGVPGVGVPGVGVPGVGVPGVGVPGVGVPGVGVPGVGVPGVGVPGVGVPGALSPAATAKAAAKAAKFGARGAVGIGGIPTFGLGPGGFPGIGDAAAAPAAAAAKAAKIGAGGVGALGGVVPGAPGAIPGLPGVGGVPGVGIPAAAAAKAAAKAAQFGLGPGVGVAPGVGVVPGVGVVPGVGVAPGIGLGPGGVIGAGVPAAAKSAAKAAAKAQFRAAAGLPAGVPGLGVGAGVPGLGVGAGVPGLGVGAGVPGPGAVPGTLAAAKAAKFGPGGVGALGGVGDLGGAGIPGGVAGVVPAAAAAAKAAAKAAQFGLGGVGGLGVGGLGAVPGAVGLGGVSPAAAAKAAKFGAAGLGGVLGAGQPFPIGGGAGGLGVGGKPPKPFGGALGALGFPGGACLGKSCGRKRK bgal_human Beta-galactosidase ELNR1 Acid beta-galactosidase Elastin receptor 1 GLB1 protein protein human Homo sapiens Human MPGFLVRILPLLLVLLLLGPTRGLRNATQRMFEIDYSRDSFLKDGQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDHDVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDKWLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLFTTDGAHKTFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDHWGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV eln-glb1 Elastin contaning complex from Human fibroblast cells 505503 N/A 505506 unspecified role unspecified role prey prey N/A 505507 unspecified role unspecified role prey prey N/A 505508 unspecified role unspecified role bait bait N/A 505509 unspecified role unspecified role prey prey association association false false false Figure 1A From the reference given in the paper the identity of the elastin protein was deteremined as P04985. eln-neu1 Elastin and Neu1 protein colocalise in arotic smooth muscle cells 505504 N/A 505506 unspecified role unspecified role neutral component neutral component N/A 505509 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 2C The proteins colocalised to the surface of non-fixed human aortic smooth muscle cells. eln-neu1-1 Elastin and Neu1 protein colocalise in arotic smooth muscle cells 505505 N/A 505506 unspecified role unspecified role neutral component neutral component N/A 505509 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 3D Proteins colocalised to the cell surface of aortic smooth muscle cells.