L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate
bicarbonate and glutamine combine to form carbamoyl phosphate
carbamoyl phosphate + L-aspartate <=> N-carbamoyl L-aspartate + orthophosphate
carbamoyl phosphate and L-aspartate combine to form N-Carbamoyl-L-aspartate
N-carbamoyl L-aspartate + H+ <=> (S)-dihydroorotate + H2O
condensation of N-Carbamoyl-L-aspartate to form (S)-Dihydroorotate (ring closure)
(S)-dihydroorotate + ubiquinone => orotate + ubiquinol
oxidation of (S)-Dihydroorotate to form Orotate
orotate + 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) <=> orotidine 5'-monophosphate (OMP) + pyrophosphate
phosphoribosylation of orotate to form oritidine 5'-phosphate (OMP)
decarboxylation of Orotidine 5'-phosphate to form Uridine 5'-phosphate
orotidine 5'-monophosphate => uridine 5'-monophosphate + CO2
dCMP + H2O => dUMP + NH4+
deamination of dCMP to form dUMP
dUTP + H2O => dUMP + pyrophosphate
hydrolysis of 2'-deoxyuridine 5'-triphosphate to form 2'-deoxyuridine 5'-phosphate
conversion of dUMP to dTMP
dUMP + N5,N10-methylene tetrahydrofolate => TMP + dihydrofolate
Pyrimidine biosynthesis