Prefix: : <http://purl.obolibrary.org/obo/>
Prefix: i: <http://purl.obolibrary.org/obo/test/inst_>
Prefix: dc: <http://purl.org/dc/elements/1.1/>
Prefix: owl: <http://www.w3.org/2002/07/owl#>
Prefix: rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#>
Prefix: xml: <http://www.w3.org/XML/1998/namespace>
Prefix: xsd: <http://www.w3.org/2001/XMLSchema#>
Prefix: rdfs: <http://www.w3.org/2000/01/rdf-schema#>



Ontology: <http://purl.obolibrary.org/obo/go/examples/change-me.owl>

Import: <http://purl.obolibrary.org/obo/wbbt.owl>
Import: <http://purl.obolibrary.org/obo/go.owl>
Import: <http://purl.obolibrary.org/obo/go/extensions/lego-relations.owl>
Import: <http://purl.obolibrary.org/obo/omeo/Caenorhabditis_elegans-uniprot-labels.owl>

Annotations: 
    rdfs:comment "PMID 21771813

Microtubule-based localization of a synaptic calcium- signaling complex is required for left-right neuronal
asymmetry in C. elegans
Chieh Chang1, Yi-Wen Hsieh1, Bluma J. Lesch2, Cornelia I. Bargmann2 and Chiou-Fen Chuang1,*

SUMMARY
The axons of C. elegans left and right AWC olfactory neurons communicate at synapses through a calcium-signaling complex to regulate stochastic asymmetric cell identities called AWCON and AWCOFF. However, it is not known how the calcium-signaling complex, which consists of UNC-43/CaMKII, TIR-1/SARM adaptor protein and NSY-1/ASK1 MAPKKK, is localized to postsynaptic sites in the AWC axons for this lateral interaction. Here, we show that microtubule-based localization of the TIR-1 signaling complex to the synapses regulates AWC asymmetry. Similar to unc-43, tir-1 and nsy-1 loss-of-function mutants, specific disruption of microtubules in AWC by nocodazole generates two AWCON neurons. Reduced localization of UNC-43, TIR-1 and NSY-1 proteins in the AWC axons strongly correlates with the 2AWCON phenotype in nocodazole-treated animals. We identified kinesin motor unc-104/kif1a mutants for enhancement of the 2AWCON phenotype of a hypomorphic tir-1 mutant. Mutations in unc-104, like microtubule depolymerization, lead to a reduced level of UNC-43, TIR-1 and NSY-1 proteins in the AWC axons. In addition,
dynamic transport of TIR-1 in the AWC axons is dependent on unc-104, the primary motor required for the transport of presynaptic vesicles. Furthermore, unc-104 acts non-cell autonomously in the AWCON neuron to regulate the AWCOFF identity. Together, these results suggest a model in which UNC-104 may transport some unknown presynaptic factor(s) in the future AWCON cell that non-cell autonomously control the trafficking of the TIR-1 signaling complex to postsynaptic regions of the AWC axons to regulate the AWCOFF identity."

AnnotationProperty: rdfs:label

    
AnnotationProperty: rdfs:comment

    
Datatype: rdf:PlainLiteral

    
ObjectProperty: BFO_0000066

    
ObjectProperty: directly_activates

    
ObjectProperty: RO_0002212

    
ObjectProperty: RO_0002213

    
ObjectProperty: has_input

    
ObjectProperty: has_output

    
ObjectProperty: indirectly_disables_action_of

    
ObjectProperty: enabled_by

    
ObjectProperty: occurs_in

    
ObjectProperty: activates

    
ObjectProperty: BFO_0000050

    
ObjectProperty: directly_inhibits

    
Class: WBbt_0005672

    
Class: GO_0019901

    
Class: GO_0030424

    
Class: UniProtKB_E0AHC6

    
Class: GO_0001078

    
Class: UniProtKB_H2KYW5

    
Class: GO_0030182

    
Class: GO_0034613

    
Class: UniProtKB_Q965R3

    
Class: UniProtKB_Q9N419

    
Class: UniProtKB_G5EDF7

    
Class: UniProtKB_P23678

    
Class: GO_0045596

    
Class: GO_0003700

    
Class: GO_0004871

    
Class: GO_0030154

    
Class: UniProtKB_O61715

    
Class: UniProtKB_O62305

    
Class: GO_0001708

    
Class: GO_0005634

    
Class: GO_0048675

    
Class: GO_0004709

    
Class: GO_0004708

    
Class: WBbt_0003826

    
Class: WBbt_0003827

    
Class: UniProtKB_G4SFC3

    
Class: GO_0005515

    
Class: UniProtKB_Q21029

    
Class: GO_0005200

    
Class: GO_0003777

    
Class: UniProtKB_Q86DA5

    
Class: GO_0004672

    
Class: GO_0032092

    
Class: GO_0060581

    
Class: GO_0004674

    
Class: GO_0035545

    
Class: GO_0005243

    SubClassOf: 
        occurs_in some GO_0005921
    
    
Class: GO_0003674

    
Class: GO_0005262

    
Class: GO_0005923

    
Class: GO_0060573

    
Class: UniProtKB_Q18817

    
Class: GO_0005921

    
Class: UniProtKB_G5EF54

    
Class: GO_0016337

    
Class: GO_0015267

    
Class: WBbt_0005832

    
Class: WBbt_0005833

    
Individual: DPH_4000010

    Annotations: 
        rdfs:label "unc-2-calcium channel activity in AWC-ON"
    
    Types: 
        GO_0005262,
        enabled_by some UniProtKB_G4SFC3,
        BFO_0000066 some WBbt_0005832
    
    
Individual: DPH_4000012

    Annotations: 
        rdfs:label "microtubule organization in AWC-OFF"
    
    Types: 
        GO_0005200,
        enabled_by some UniProtKB_Q18817
    
    Facts:  
     BFO_0000050  DPH_4000003
    
    
Individual: DPH_4000011

    Annotations: 
        rdfs:label "AWC axon outgrowth"
    
    Types: 
        BFO_0000066 some WBbt_0005672,
        GO_0048675
    
    
Individual: LEGO_PMID_21771813_0000016

    Annotations: 
        rdfs:label "unc-76 activity"@en
    
    Types: 
        GO_0004871,
        enabled_by some UniProtKB_E0AHC6,
        BFO_0000066 some WBbt_0003827,
        BFO_0000066 some GO_0030424
    
    Facts:  
     directly_activates  LEGO_PMID_21771813_0000024,
     directly_activates  LEGO_PMID_21771813_0000023,
     BFO_0000050  DPH_4000011,
     BFO_0000050  DPH_4000001
    
    
Individual: LEGO_PMID_21771813_0000019

    Annotations: 
        rdfs:label "nsy-1-MAPKKK activity"@en
    
    Types: 
        BFO_0000066 some WBbt_0003827,
        BFO_0000066 some GO_0030424,
        GO_0004674,
        enabled_by some UniProtKB_Q21029
    
    Facts:  
     directly_activates  LEGO_PMID_21771813_0000018,
     BFO_0000050  DPH_4000003
    
    
Individual: LEGO_PMID_21771813_0000018

    Annotations: 
        rdfs:label "sek-1-MAPKK-activity"@en
    
    Types: 
        GO_0004674,
        enabled_by some UniProtKB_G5EDF7
    
    Facts:  
     directly_activates  LEGO_PMID_21771813_0000026,
     directly_inhibits  LEGO_PMID_21771813_0000025,
     BFO_0000050  DPH_4000004,
     BFO_0000050  DPH_4000003
    
    
Individual: DPH_4000005

    Annotations: 
        rdfs:label "srsx-3 -> AWC-OFF"
    
    Types: 
        occurs_in some WBbt_0005833,
        enabled_by some UniProtKB_Q965R3,
        GO_0003674
    
    Facts:  
     BFO_0000050  DPH_4000004
    
    
Individual: DPH_4000004

    Annotations: 
        rdfs:label "negative regulation of AWC-ON differentiation in AWC-OFF cell"
    
    Types: 
        occurs_in some WBbt_0005833,
        GO_0045596
    
    Facts:  
     RO_0002212  DPH_4000007
    
    
Individual: DPH_4000007

    Annotations: 
        rdfs:label "AWC-ON cell differentiation from AWCR"
    
    Types: 
        GO_0030182,
        has_input some WBbt_0003826,
        has_output some WBbt_0005832
    
    
Individual: DPH_4000006

    Annotations: 
        rdfs:label "AWC-OFF cell differentiation from AWCL"
    
    Types: 
        GO_0030182,
        has_output some WBbt_0005833,
        has_input some WBbt_0003827
    
    
Individual: DPH_4000009

    Annotations: 
        rdfs:label "axonal localization of Unc-43, NSY-1, TIR-1"
    
    Types: 
        BFO_0000066 some WBbt_0003827,
        BFO_0000066 some GO_0030424,
        GO_0034613
    
    
Individual: DPH_4000008

    Annotations: 
        rdfs:label "unc-104 activity"
    
    Types: 
        BFO_0000066 some WBbt_0003826,
        GO_0003777,
        enabled_by some UniProtKB_P23678
    
    Facts:  
     BFO_0000050  DPH_4000003,
     BFO_0000050  DPH_4000009
    
    
Individual: DPH_4000001

    Annotations: 
        rdfs:label "negative regulation of AWC-OFF differentiation in AWC-ON cell"
    
    Types: 
        occurs_in some WBbt_0005832,
        GO_0045596
    
    Facts:  
     RO_0002212  DPH_4000006
    
    
Individual: DPH_4000000

    Annotations: 
        rdfs:label "nsy-7 as transcription repressor of srsx-3"
    
    Types: 
        enabled_by some UniProtKB_H2KYW5,
        occurs_in some WBbt_0005833,
        occurs_in some GO_0005634,
        GO_0001078
    
    Facts:  
     BFO_0000050  DPH_4000001,
     RO_0002212  DPH_4000005
    
    
Individual: DPH_4000003

    Annotations: 
        rdfs:label "cell fate commitment of AWC-OFF cell"
    
    Types: 
        occurs_in some WBbt_0003827,
        GO_0060581,
        has_output some WBbt_0005833
    
    Facts:  
     BFO_0000050  DPH_4000006,
     BFO_0000050  LEGO_PMID_21771813_0000027
    
    
Individual: DPH_4000002

    Annotations: 
        rdfs:label "cell fate specification of AWC-ON cell"
    
    Types: 
        occurs_in some WBbt_0003826,
        GO_0060581,
        has_output some WBbt_0005832
    
    Facts:  
     BFO_0000050  DPH_4000007,
     BFO_0000050  LEGO_PMID_21771813_0000027
    
    
Individual: LEGO_PMID_21771813_0000020

    Annotations: 
        rdfs:label "tir-1 activity"@en,
        rdfs:comment "Here, we show that microtubule-based localization of the TIR-1 signaling complex to the synapses regulates AWC asymmetry"
    
    Types: 
        GO_0019901,
        BFO_0000050 some GO_0032092,
        enabled_by some UniProtKB_Q86DA5
    
    Facts:  
     directly_activates  LEGO_PMID_21771813_0000021,
     directly_activates  LEGO_PMID_21771813_0000019,
     BFO_0000050  DPH_4000003
    
    
Individual: LEGO_PMID_21771813_0000024

    Annotations: 
        rdfs:label "claudin activity"@en,
        rdfs:comment "establishment of cell fate?"
    
    Types: 
        GO_0005515,
        BFO_0000066 some WBbt_0003826,
        enabled_by some UniProtKB_Q9N419,
        occurs_in some GO_0005923,
        BFO_0000050 some GO_0016337
    
    Facts:  
     BFO_0000050  DPH_4000001,
     directly_inhibits  DPH_4000010
    
    
Individual: LEGO_PMID_21771813_0000023

    Annotations: 
        rdfs:label "innexin activity"@en,
        rdfs:comment "establishment of cell fate?"
    
    Types: 
        BFO_0000066 some WBbt_0003826,
        GO_0005243,
        enabled_by some UniProtKB_O61715
    
    Facts:  
     BFO_0000050  DPH_4000001,
     directly_inhibits  DPH_4000010
    
    
Individual: LEGO_PMID_21771813_0000022

    Annotations: 
        rdfs:label "unc-2-calcium channel activity in AWC-OFF"@en
    
    Types: 
        GO_0005262,
        BFO_0000066 some WBbt_0003827,
        enabled_by some UniProtKB_G4SFC3
    
    Facts:  
     directly_activates  LEGO_PMID_21771813_0000021,
     BFO_0000050  DPH_4000003
    
    
Individual: LEGO_PMID_21771813_0000021

    Annotations: 
        rdfs:label "CaMKII/UNC-43 activity"@en
    
    Types: 
        enabled_by some UniProtKB_O62305,
        BFO_0000066 some WBbt_0003827,
        GO_0004672,
        BFO_0000066 some GO_0030424
    
    Facts:  
     BFO_0000050  DPH_4000003,
     directly_activates  LEGO_PMID_21771813_0000019
    
    
Individual: LEGO_PMID_21771813_0000028

    Annotations: 
        rdfs:comment "check: no 'maintenance of cell identity' in GO. Existing GO annotation is to 'neuron fate specification'.

GO:0001708 ! cell fate specification [DEF: \"The process involved in the specification of cell identity. Once specification has taken place, a cell will be committed to differentiate down a specific pathway if left in its normal environment.\"]",
        rdfs:label "nsy-7 activity"@en,
        rdfs:comment "NSY-7 likely acts as a transcriptional regulator that represses expression of AWC-OFF genes, such as srsx-3 and hlh-11, in the AWC-ON cell; in vitro, NSY-7 displays sequence-specific, DNA-binding activity to elements found within the promoter regions of srsx-3 and hlh-11; consistent with its role as a transcriptional regulator, a NSY-7::GFP reporter localizes to the AWC nucleus"
    
    Types: 
        enabled_by some UniProtKB_H2KYW5,
        GO_0003674,
        BFO_0000066 some WBbt_0005832
    
    Facts:  
     RO_0002213  LEGO_PMID_21771813_0000025,
     BFO_0000050  DPH_4000002
    
    
Individual: LEGO_PMID_21771813_0000027

    Annotations: 
        rdfs:label "l/r asymmetry"@en
    
    Types: 
        GO_0035545
    
    
Individual: LEGO_PMID_21771813_0000026

    Annotations: 
        rdfs:label "srsx-3 -> AWCL"@en,
        rdfs:comment "Troemel et al. 1999; Bauer Huang et al. 2007"
    
    Types: 
        BFO_0000066 some WBbt_0003827,
        enabled_by some UniProtKB_Q965R3,
        GO_0003674
    
    Facts:  
     BFO_0000050  DPH_4000006
    
    
Individual: LEGO_PMID_21771813_0000025

    Annotations: 
        rdfs:label "str-2 -> AWC-ON"@en,
        rdfs:comment "Troemel et al. 1999; Bauer Huang et al. 2007"
    
    Types: 
        occurs_in some WBbt_0005832,
        enabled_by some UniProtKB_G5EF54,
        GO_0003674
    
    Facts:  
     BFO_0000050  DPH_4000001,
     BFO_0000050  DPH_4000007