Prefix: : <http://purl.obolibrary.org/obo/>
Prefix: i: <http://purl.obolibrary.org/obo/test/inst_>
Prefix: dc: <http://purl.org/dc/elements/1.1/>
Prefix: owl: <http://www.w3.org/2002/07/owl#>
Prefix: rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#>
Prefix: xml: <http://www.w3.org/XML/1998/namespace>
Prefix: xsd: <http://www.w3.org/2001/XMLSchema#>
Prefix: rdfs: <http://www.w3.org/2000/01/rdf-schema#>
Ontology: <http://purl.obolibrary.org/obo/go/examples/change-me.owl>
Import: <http://purl.obolibrary.org/obo/wbbt.owl>
Import: <http://purl.obolibrary.org/obo/go.owl>
Import: <http://purl.obolibrary.org/obo/go/extensions/lego-relations.owl>
Import: <http://purl.obolibrary.org/obo/omeo/Caenorhabditis_elegans-uniprot-labels.owl>
Annotations:
rdfs:comment "PMID 21771813
Microtubule-based localization of a synaptic calcium- signaling complex is required for left-right neuronal
asymmetry in C. elegans
Chieh Chang1, Yi-Wen Hsieh1, Bluma J. Lesch2, Cornelia I. Bargmann2 and Chiou-Fen Chuang1,*
SUMMARY
The axons of C. elegans left and right AWC olfactory neurons communicate at synapses through a calcium-signaling complex to regulate stochastic asymmetric cell identities called AWCON and AWCOFF. However, it is not known how the calcium-signaling complex, which consists of UNC-43/CaMKII, TIR-1/SARM adaptor protein and NSY-1/ASK1 MAPKKK, is localized to postsynaptic sites in the AWC axons for this lateral interaction. Here, we show that microtubule-based localization of the TIR-1 signaling complex to the synapses regulates AWC asymmetry. Similar to unc-43, tir-1 and nsy-1 loss-of-function mutants, specific disruption of microtubules in AWC by nocodazole generates two AWCON neurons. Reduced localization of UNC-43, TIR-1 and NSY-1 proteins in the AWC axons strongly correlates with the 2AWCON phenotype in nocodazole-treated animals. We identified kinesin motor unc-104/kif1a mutants for enhancement of the 2AWCON phenotype of a hypomorphic tir-1 mutant. Mutations in unc-104, like microtubule depolymerization, lead to a reduced level of UNC-43, TIR-1 and NSY-1 proteins in the AWC axons. In addition,
dynamic transport of TIR-1 in the AWC axons is dependent on unc-104, the primary motor required for the transport of presynaptic vesicles. Furthermore, unc-104 acts non-cell autonomously in the AWCON neuron to regulate the AWCOFF identity. Together, these results suggest a model in which UNC-104 may transport some unknown presynaptic factor(s) in the future AWCON cell that non-cell autonomously control the trafficking of the TIR-1 signaling complex to postsynaptic regions of the AWC axons to regulate the AWCOFF identity."
AnnotationProperty: rdfs:label
AnnotationProperty: rdfs:comment
Datatype: rdf:PlainLiteral
ObjectProperty: BFO_0000066
ObjectProperty: directly_activates
ObjectProperty: enabled_by
ObjectProperty: occurs_in
ObjectProperty: activates
ObjectProperty: BFO_0000050
ObjectProperty: directly_inhibits
Class: WBbt_0005672
Class: GO_0030424
Class: GO_0019901
Class: UniProtKB_E0AHC6
Class: UniProtKB_H2KYW5
Class: GO_0045892
Class: UniProtKB_Q965R3
Class: UniProtKB_Q9N419
Class: UniProtKB_G5EDF7
Class: GO_0045596
Class: GO_0003700
Class: GO_0004871
Class: UniProtKB_O61715
Class: UniProtKB_O62305
Class: GO_0001708
Class: GO_0006355
Class: GO_0004709
Class: GO_0004708
Class: UniProtKB_G4SFC3
Class: UniProtKB_Q21029
Class: UniProtKB_Q86DA5
Class: GO_0004672
Class: GO_0004674
Class: GO_0035545
Class: GO_0005243
SubClassOf:
occurs_in some GO_0005921
Class: GO_0003674
Class: GO_0005262
Class: GO_0005923
Class: GO_0005921
Class: UniProtKB_G5EF54
Class: GO_0015267
Class: WBbt_0005832
Class: WBbt_0005833
Class: GO_0000981
Individual: <http://purl.obolibrary.org/obo/go/examples/change-me.owl#neg_reg_cell_diff_AWCOFF>
Types:
BFO_0000066 some WBbt_0005833,
GO_0045596
Facts:
BFO_0000050 LEGO_PMID_21771813_0000027
Individual: <http://purl.obolibrary.org/obo/go/examples/change-me.owl#neg_reg_cell_diff_AWCON>
Types:
BFO_0000066 some WBbt_0005832,
GO_0045596
Facts:
BFO_0000050 LEGO_PMID_21771813_0000027
Individual: <http://purl.obolibrary.org/obo/go/examples/change-me.owl#sek-1_clone>
Types:
GO_0004674,
enabled_by some UniProtKB_G5EDF7
Facts:
BFO_0000050 <http://purl.obolibrary.org/obo/go/examples/change-me.owl#neg_reg_cell_diff_AWCON>,
directly_inhibits LEGO_PMID_21771813_0000025
Individual: <http://purl.obolibrary.org/obo/go/examples/change-me.owl#nsy-7_activity_clone>
Types:
enabled_by some UniProtKB_H2KYW5,
GO_0000981
Facts:
directly_inhibits LEGO_PMID_21771813_0000026,
BFO_0000050 <http://purl.obolibrary.org/obo/go/examples/change-me.owl#neg_reg_cell_diff_AWCOFF>,
BFO_0000050 <http://purl.obolibrary.org/obo/go/examples/change-me.owl#negative_regulation_of_tsc>
Individual: LEGO_PMID_21771813_0000020
Annotations:
rdfs:label "tir-1 activity"@en,
rdfs:comment "Here, we show that microtubule-based localization of the TIR-1 signaling complex to the synapses regulates AWC asymmetry"
Types:
GO_0004672,
enabled_by some UniProtKB_Q86DA5
Facts:
BFO_0000050 LEGO_PMID_21771813_0000017,
directly_activates LEGO_PMID_21771813_0000019
Individual: LEGO_PMID_21771813_0000024
Annotations:
rdfs:label "claudin activity"@en,
rdfs:comment "establishment of cell fate?"
Types:
enabled_by some UniProtKB_Q9N419,
occurs_in some GO_0005923,
GO_0015267
Facts:
directly_inhibits LEGO_PMID_21771813_0000022
Individual: LEGO_PMID_21771813_0000023
Annotations:
rdfs:label "innexin activity"@en,
rdfs:comment "establishment of cell fate?"
Types:
GO_0005243,
enabled_by some UniProtKB_O61715
Facts:
directly_inhibits LEGO_PMID_21771813_0000022,
BFO_0000050 LEGO_PMID_21771813_0000017
Individual: LEGO_PMID_21771813_0000022
Annotations:
rdfs:label "unc-2-calcium channel activity"@en
Types:
GO_0005262,
enabled_by some UniProtKB_G4SFC3
Facts:
directly_activates LEGO_PMID_21771813_0000021,
BFO_0000050 LEGO_PMID_21771813_0000017
Individual: LEGO_PMID_21771813_0000021
Annotations:
rdfs:label "CaMKII/UNC-43 activity"@en
Types:
GO_0019901,
enabled_by some UniProtKB_O62305
Facts:
directly_activates LEGO_PMID_21771813_0000020,
BFO_0000050 LEGO_PMID_21771813_0000017
Individual: <http://purl.obolibrary.org/obo/go/examples/change-me.owl#negative_regulation_of_tsc>
Types:
GO_0045892
Individual: LEGO_PMID_21771813_0000028
Annotations:
rdfs:comment "check: no 'maintenance of cell identity' in GO. Existing GO annotation is to 'neuron fate specification'.
GO:0001708 ! cell fate specification [DEF: \"The process involved in the specification of cell identity. Once specification has taken place, a cell will be committed to differentiate down a specific pathway if left in its normal environment.\"]",
rdfs:label "nsy-7 activity"@en,
rdfs:comment "NSY-7 likely acts as a transcriptional regulator that represses expression of AWC-OFF genes, such as srsx-3 and hlh-11, in the AWC-ON cell; in vitro, NSY-7 displays sequence-specific, DNA-binding activity to elements found within the promoter regions of srsx-3 and hlh-11; consistent with its role as a transcriptional regulator, a NSY-7::GFP reporter localizes to the AWC nucleus"
Types:
enabled_by some UniProtKB_H2KYW5,
GO_0000981
Facts:
BFO_0000050 <http://purl.obolibrary.org/obo/go/examples/change-me.owl#cell_fate_spec_AWCON>,
BFO_0000050 <http://purl.obolibrary.org/obo/go/examples/change-me.owl#reg_of_tsc>,
activates LEGO_PMID_21771813_0000025
Individual: <http://purl.obolibrary.org/obo/go/examples/change-me.owl#cell_fate_spec_AWCON>
Types:
GO_0001708,
BFO_0000066 some WBbt_0005832
Facts:
BFO_0000050 LEGO_PMID_21771813_0000027
Individual: LEGO_PMID_21771813_0000027
Annotations:
rdfs:label "l/r asymmetry"@en
Types:
GO_0035545
Individual: LEGO_PMID_21771813_0000017
Annotations:
rdfs:label "establishment of cell fate"@en
Types:
occurs_in some WBbt_0005672,
GO_0001708
Facts:
BFO_0000050 LEGO_PMID_21771813_0000027
Individual: LEGO_PMID_21771813_0000026
Annotations:
rdfs:label "srsx-3 -> AWC-OFF"@en,
rdfs:comment "Troemel et al. 1999; Bauer Huang et al. 2007"
Types:
occurs_in some WBbt_0005833,
enabled_by some UniProtKB_Q965R3,
BFO_0000050 some
(GO_0001708
and (occurs_in some WBbt_0005833)),
GO_0003674
Individual: <http://purl.obolibrary.org/obo/go/examples/change-me.owl#cell_fate_spec_AWCOFF>
Types:
GO_0001708,
BFO_0000066 some WBbt_0005833
Facts:
BFO_0000050 LEGO_PMID_21771813_0000027
Individual: LEGO_PMID_21771813_0000016
Annotations:
rdfs:label "unc-76 activity"@en
Types:
GO_0004871,
enabled_by some UniProtKB_E0AHC6,
BFO_0000066 some GO_0030424
Facts:
directly_activates LEGO_PMID_21771813_0000024,
BFO_0000050 LEGO_PMID_21771813_0000017,
directly_activates LEGO_PMID_21771813_0000023
Individual: LEGO_PMID_21771813_0000025
Annotations:
rdfs:label "str-2 -> AWC-ON"@en,
rdfs:comment "Troemel et al. 1999; Bauer Huang et al. 2007"
Types:
occurs_in some WBbt_0005832,
enabled_by some UniProtKB_G5EF54,
BFO_0000050 some
(GO_0001708
and (occurs_in some WBbt_0005832)),
GO_0003674
Individual: <http://purl.obolibrary.org/obo/go/examples/change-me.owl#reg_of_tsc>
Types:
GO_0006355
Individual: LEGO_PMID_21771813_0000019
Annotations:
rdfs:label "nsy-1-MAPKKK activity"@en
Types:
GO_0004674,
enabled_by some UniProtKB_Q21029
Facts:
directly_activates LEGO_PMID_21771813_0000018,
BFO_0000050 LEGO_PMID_21771813_0000017,
directly_activates <http://purl.obolibrary.org/obo/go/examples/change-me.owl#sek-1_clone>
Individual: LEGO_PMID_21771813_0000018
Annotations:
rdfs:label "sek-1-MAPKK-activity"@en
Types:
GO_0004674,
enabled_by some UniProtKB_G5EDF7
Facts:
directly_activates LEGO_PMID_21771813_0000026,
BFO_0000050 <http://purl.obolibrary.org/obo/go/examples/change-me.owl#cell_fate_spec_AWCOFF>