# HISTORY
24 Mar 2016: Updated by: TOUCHUP-v1.15
14 Mar 2016: Updated by: TOUCHUP-v1.12

# molecular_function

# cellular_component

# biological_process

# WARNINGS - THE FOLLOWING HAVE BEEN REMOVED FOR THE REASONS NOTED

# NOTES
Molecular Function: PTHR10188 has both Taspase (peptidase activity) and asparaginase activities. Based on sequence alignments shown in Figure 3 of PMID: 14636557, LDTVG is the conserved motif for taspase activity and hence threonine peptidase activity (GO:4298) was propagated to the AN3 node. HFTIGM is the conserved motif for glycosylasparaginase and hence GO:3948 was propagated to AN50 node. And the AN98 node has an asparginase motif  and hence GO:4067 was propagated.
PTHR10188_AN155 Family has the EColi iaaA gene annotated to both peptidase and asparaginase activity and hence those both annotations were propagated to that family.  As these appear in different eukaryotic clades the dual activity was most likely ancestral, so annotated root, with loss of one or more function in each descendant clade.
Biological Process: Propagated GO:33345 (asparagine catabolic process via L-aspartate ) to root but lost in clades without asparaginase activity.  Protein maturation is annotated for mouse Aga, but the paper indicates that Aga is processed (i.e. it does not do the processing) so this annotation is suspect.  Protein deglycosylation was propagated to the root of the Aga clade.
Component: Based on annotations plus SwissProt annotation of human asparaginase to cytosol, inferred cytosol is ancestral and glycosylasparaginase targeting to the lysosome evolved at the base of that clade.

# REFERENCE
Annotation inferences using phylogenetic trees
The goal of the GO Reference Genome Project, described in PMID 19578431, is to provide accurate, complete and consistent GO annotations for all genes in twelve model organism genomes. To this end, GO curators are annotating evolutionary trees from the PANTHER database with GO terms describing molecular function, biological process and cellular component. GO terms based on experimental data from the scientific literature are used to annotate ancestral genes in the phylogenetic tree by sequence similarity (ISS), and unannotated descendants of these ancestral genes are inferred to have inherited these same GO annotations by descent. The annotations are done using a tool called PAINT (Phylogenetic Annotation and INference Tool).