GO Tutorial: Annotating A Paper

Exercise IV

Annotation - Answers

Mutants with defects in RNA binding protein (RBP) are defective in cell growth and differentiation. An example RBP that regulates development is provided by the Bruno protein and its role as a translational repressor of oskar mRNA. In Drosophila, oskar is required for formation of germ cells and positioning of the posterior of the embryo.

Text string GO Ontology GO Term GO ID
RNA binding function RNA binding GO:0003723
cell growth process cell growth GO:0016049
differentiation process cell differentiation GO:0030154
translational repressor of oskar mRNA process negative regulation of oskar mRNA translation GO:0007319
translational repressor of oskar mRNA function translation repressor activity GO:0030371
formation of germ cells process germ-cell development GO:0007281

 


 

Using microarray analysis, we identified RERG (ras-related and estrogen-regulated growth inhibitor). Like Ras, RERG protein exhibited intrinsic GDP/GTP binding and GTP hydrolysis activity. Unlike Ras proteins, RERG lacks a known recognition site for COOH-terminal prenylation and was localized primarily in the cytoplasm.

Text string GO Ontology GO Term GO ID
estrogen-regulated process response to hormone stimulus GO:0009725
growth inhibitor process negative regulation of cell growth GO:0030308
Ras, GDP/GTP binding process small GTPase mediated signal transduction GO:0007624
GDP/GTP binding function GDP binding GO:0019003
GDP/GTP binding function GTP binding GO:0005525
GTP hydrolysis function GTPase activity GO:0003924
cytoplasm component cytoplasm

 


Back to exercise IV

Amelia Ireland