GO Annotation File Example

The file format of a gene association file, along with sample annotations, is shown below. Red text denotes required fields; the rows with column numbers are not part of the file, but are included here for convenience in viewing.

In the annotation files, each annotation (association of a single gene product with a single GO term) takes up one line, so the contents columns 1 through 15 would appear on a single line. They have been split up here for ease of viewing.

1.
Database (DB)
[required]
2.
DB Object ID
[required]
3.
DB Object Symbol
[required]
4.
Qualifier
[optional]
5.
GO ID
[required]
SGD S000000296 PHO3 GO:0006796
SGD S000000296 PHO3 GO:0003993
SGD S000005370 RCL1 NOT GO:0003963
SGD S000005197 TEX1 GO:0006406
SGD S000005316 ABZ1 GO:0046820
6.
DB:Reference (|DB:Reference)
[required]
7.
Evidence Code
[required]
8.
With/From
[optional]
9.
Aspect
[required]
10.
DB Object Name (|Name)
[optional]
SGD_REF:S000047115|PMID:2407294 TAS P acid phosphatase
SGD_REF:S000047115|PMID:2407294 IMP F acid phosphatase
SGD_REF:S000039255|PMID:10790377 IDA F
SGD_REF:S000069956|PMID:11979277 IC GO:0000346 P
SGD_REF:S000057703|PMID:8346682 ISS CGSC:pabA|CGSC:pabB F aminodeoxychorismate synthase
11.
DB Object Synonym (|Synonym)
[optional]
12.
DB Object Type
[required]
13.
Taxon (|Taxon)
[required]
14.
Date
[required]
15.
Assigned by
[required]
YBR092C gene taxon:4932 20010118 SGD
YBR092C gene taxon:4932 20041220 SGD
YOL010W gene taxon:4932 20020530 SGD
YNL253W gene taxon:4932 20030221 SGD
YNR033W gene taxon:4932 20030106 SGD

The above entries as they appear in the actual file:

SGD	S000000296	PHO3		GO:0003993	SGD_REF:S000047763|PMID:2407294 	IMP		F	acid phosphatase	YBR092C	gene	taxon:4932	20010118	SGD
SGD	S000000296	PHO3		GO:0006796	SGD_REF:S000047115|PMID:2407294 	TAS		P	acid phosphatase	YBR092C	gene	taxon:4932	20041220	SGD
SGD	S000005370	RCL1	NOT	GO:0003963	SGD_REF:S000039255|PMID:10790377	IDA		F		YOL010W	gene	taxon:4932	20020530	SGD
SGD	S000005197	TEX1		GO:0006406	SGD_REF:S000069956|PMID:11979277	IC	GO:0000346	P		YNL253W	gene	taxon:4932	20030221	SGD
SGD	S000005316	ABZ1		GO:0046820	SGD_REF:S000057703|PMID:8346682 	ISS	CGSC:pabA|CGSC:pabB	F	aminodeoxychorismate synthase	YNR033W	gene	taxon:4932	20030106	SGD

Return to GO annotation file format guide