26th September 2003, Bar Harbor
Pathogen Sequencing Unit
Wellcome Trust Sanger Institute
GO annotations in GeneDB 

	process	component	function	total	
	curated	all	curated	all	curated	all	curated 	all	
Aspergillus fumigatus	207	207	126	126	224	224	557	557	
Dictyostelium discoideum	0	4342	0	1922	0	5535	0	11799	
Glossina morsitans	0	1279	0	1221	0	2364	0	4864	
Leishmania major	97	1168	73	572	139	1584	309	3324	
Plasmodium berghei	0	1011	0	898	0	1189	0	3098	
Plasmodium chabaudi	0	1944	0	1742	0	2300	0	5986	
Plasmodium falciparum	2282	2282	2493	2493	2350	2350	7126	7126	
Trypanosoma brucei*	673	1989	700	1114	600	2636	1973	5739	

*Sanger and TIGR pooled annotation

•	Amigo installed locally and accessible via entry page for GeneDB (http://www.genedb.org)
o	all in-house annotations can be browsed
o	Gene Ontology Home page and main Amigo site are out of synch:
•	number of gene associations out of date
•	many extra data sources listed locally or listed differently
e.g. Sanger and TIGR data sets from T. brucei are combined

•	Malaria curator has been hired.
Other news
•	GOTCHA - an automated GO prediction tool by David Martin - has been tested on the manually annotated P. falciparum dataset from 2002.  It will be submitted for publication soon and results look promising.  In many cases, “false positives” were appeared correct after subsequent manual review.  

•	The genomes of three related parasites, Trypanosoma brucei, T. cruzi and Leishmania major, are close to completion.  They will be annotated together mainly by Sanger and TIGR.  “Best guess” GO terms will be provided by GOTCHA and manually reviewed to provide full (ISS) annotation for L. major and T. brucei (16,000 genes).

•	collaborators in South America have used GO to annotate a large number of ESTs from the second most virulent human malaria parasite, Plasmodium vivax