# HISTORY 26 Apr 2016: Updated by: TOUCHUP-v1.15 19 Mar 2016: Updated by: TOUCHUP-v1.14 # molecular_function 20151025: Eukaryota_PTN000772288 has function structural constituent of nuclear pore (GO:0017056) # cellular_component 20151026: Eukaryota_PTN000772288 is found in nuclear pore inner ring (GO:0044611) # biological_process 20151026: Eukaryota_PTN000772288 participates in nuclear pore organization (GO:0006999) 20151026: Eukaryota_PTN000772288 participates in protein import into nucleus, docking (GO:0000059) # WARNINGS - THE FOLLOWING HAVE BEEN REMOVED FOR THE REASONS NOTED # NOTES This family contains a conserved inner ring nucleoporin, called NUP192 in S. cerevisiae or NUP205 in vertebrates and other species. Comment on an annotation not propagated: --------------------------------- From Kosova et al. 1999 Nup192p is a conserved nucleoporin with a preferential location at the inner site of the nuclear membrane (PMID:10428845), there are three annotations for NUP192. The BP and MF ones are fine. However, the CC one is to the term “nuclear pore nuclear basket”. While this annotation is certainly consistent with what the authors say, this is an old paper (1999). I think that Kosova et al. may have been speculating prematurely about the structural role of Nup192. Here is their summary paragraph at the end of the Intro: "To further study the interaction of Nic96p with other nuclear pore proteins in yeast, we sought to analyze the putativeSaccharomyces cerevisiae homologue (ORF YJL039c) of human p205, which is in a complex with Nup93 (18). We show here that YJL039c encodes the so far largest yeast NPC protein, which is essential for cell growth. By immunoelectron microscopy, Nup192p-ProtA was found to be localized at the nuclear basket. Temperature-sensitive mutants of nup192 reveal that the NPC reporter protein Nup49p-GFP was no longer assembled into nuclear pores. This suggests that Nup192p is a structural protein of the nuclear pores, most likely constituting a major component of the nuclear basket filaments.” I’ve also been reading a couple recent reviews (listed below). Both of them talk about Nup192 as a structural Nup in the inner ring, and neither lists it as part of the nuclear basket, so this annotation doesn’t seem consistent with current thinking on the location of Nup192 within the nuclear pore. # REFERENCE Annotation inferences using phylogenetic trees The goal of the GO Reference Genome Project, described in PMID 19578431, is to provide accurate, complete and consistent GO annotations for all genes in twelve model organism genomes. To this end, GO curators are annotating evolutionary trees from the PANTHER database with GO terms describing molecular function, biological process and cellular component. GO terms based on experimental data from the scientific literature are used to annotate ancestral genes in the phylogenetic tree by sequence similarity (ISS), and unannotated descendants of these ancestral genes are inferred to have inherited these same GO annotations by descent. The annotations are done using a tool called PAINT (Phylogenetic Annotation and INference Tool).