# HISTORY 27 Mar 2016: Updated by: TOUCHUP-v1.15 18 Nov 2015: redistribution of PTN000121726 from PTHR11107 to PTHR23389 17 Mar 2016: Updated by: TOUCHUP-v1.14 # molecular_function 20140528: Bacteria _PTN000121726 has function DNA ligase (NAD+) activity (GO:0003911) 20140528: Eukaryota_PTN000121684 has function DNA binding (GO:0003677) # cellular_component 20140528: Bacteria _PTN000121726 is found in cytosol (GO:0005829) 20140528: Eukaryota_PTN000121684 is found in nucleus (GO:0005634) # biological_process 20140528: Bacteria _PTN000121726 participates in base-excision repair, DNA ligation (GO:0006288) # WARNINGS - THE FOLLOWING HAVE BEEN REMOVED FOR THE REASONS NOTED # NOTES The bacteria branch of this family is clearly demonstrated to function as a DNA ligase; in the eukaryotic branch, there is a lack of experimental evidence to clearly show that proteins in this family are directly involved in DNA replication (human RFC1 is shown to be part of a RFC complex). # REFERENCE Annotation inferences using phylogenetic trees The goal of the GO Reference Genome Project, described in PMID 19578431, is to provide accurate, complete and consistent GO annotations for all genes in twelve model organism genomes. To this end, GO curators are annotating evolutionary trees from the PANTHER database with GO terms describing molecular function, biological process and cellular component. GO terms based on experimental data from the scientific literature are used to annotate ancestral genes in the phylogenetic tree by sequence similarity (ISS), and unannotated descendants of these ancestral genes are inferred to have inherited these same GO annotations by descent. The annotations are done using a tool called PAINT (Phylogenetic Annotation and INference Tool).