# HISTORY
26 Mar 2016: Updated by: TOUCHUP-v1.15
16 Mar 2016: Updated by: TOUCHUP-v1.14

# molecular_function
20150508: node_PTN000475663 has function alanine-glyoxylate transaminase activity (GO:0008453) 
20150508: node_PTN000475663 has function serine-pyruvate transaminase activity (GO:0004760) 
20150508: node_PTN000475583 has function O-phospho-L-serine:2-oxoglutarate aminotransferase activity (GO:0004648) 
20150508: node_PTN001465704 has LOST/MODIFIED function alanine-glyoxylate transaminase activity (GO:0008453) 
20150508: node_PTN001465704 has LOST/MODIFIED function serine-pyruvate transaminase activity (GO:0004760) 

# cellular_component
20150508: node_PTN000475663 is found in peroxisome (GO:0005777) 
20150508: node_PTN001465704 is NOT found in peroxisome (GO:0005777) 

# biological_process
20150514: node_PTN000475663 participates in glycine biosynthetic process, by transamination of glyoxylate (GO:0019265) 
20150514: node_PTN001465704 does NOT participate in glycine biosynthetic process, by transamination of glyoxylate (GO:0019265) 

# WARNINGS - THE FOLLOWING HAVE BEEN REMOVED FOR THE REASONS NOTED

# NOTES
- there are 2 major clades: PTN000475583 and PTN000475663
PTN000475583 is a phosphoserine aminotransferase group
PTN000475663 is an alanine-glyoxylate transaminase/serine-pyruvate transaminase group
both of these belong to class IV aminotransferase group
- PTN000475583:
pmid:8513804 original characterization of family
conserved residues: (yeast SER1 coordinates): 173G, 194D, 218K, 368R
PTN001465704 doesn?t have the first 3 of the conserved residues, the rest are very good.
the enzyme is a dimer
pmid:10024454 - structure for the E. coli protein
active site residues: (yeast SER1 coordinates): 45H, 46R, 80G, 112W, 170T, 194D, 196S, 197S, 217Q, 218K, 271N, 272T, 361H, 362R, 368R
the enzyme is a dimer
- PTN000475663:
PTN001465704 looks very much out of place in this clade; PTN001080594 is also very much divergent
the rat enzyme has 2 isoforms that localize to peroxisome/mitochondrion
it seems that the protein localizes to the peroxisome in all the other systems as well (in yeast it is reported in the cytosol in a http experiment)
pmid:16226833 : crystal structure for yeast AGX1 protein
REF: in mammals the protein is in both mito/peroxisome
sc enzyne: serine:pyruvate transaminase activity is only 1% of the ala:glyoxylate

# REFERENCE
Annotation inferences using phylogenetic trees
The goal of the GO Reference Genome Project, described in PMID 19578431, is to provide accurate, complete and consistent GO annotations for all genes in twelve model organism genomes. To this end, GO curators are annotating evolutionary trees from the PANTHER database with GO terms describing molecular function, biological process and cellular component. GO terms based on experimental data from the scientific literature are used to annotate ancestral genes in the phylogenetic tree by sequence similarity (ISS), and unannotated descendants of these ancestral genes are inferred to have inherited these same GO annotations by descent. The annotations are done using a tool called PAINT (Phylogenetic Annotation and INference Tool).