# HISTORY
25 Mar 2016: Updated by: TOUCHUP-v1.15
16 Mar 2016: Updated by: TOUCHUP-v1.14

# molecular_function
20150706: root_PTN000363272 has function pre-mRNA binding (GO:0036002) 
20150706: root_PTN000363272 has function U6 snRNA binding (GO:0017070) 

# cellular_component
20150706: root_PTN000363272 is found in Prp19 complex (GO:0000974) 
20150706: root_PTN000363272 is found in U2-type catalytic step 1 spliceosome (GO:0071006) 
20150706: root_PTN000363272 is found in U2-type catalytic step 2 spliceosome (GO:0071007) 

# biological_process
20150706: root_PTN000363272 participates in spliceosomal snRNP assembly (GO:0000387) 

# WARNINGS - THE FOLLOWING HAVE BEEN REMOVED FOR THE REASONS NOTED

# NOTES
This family contains CWC2 (from S. cerevisiae) which has been shown to be a part of the PRP19 complex that is involved in assembly of the spliceosome.
Comments on the tree:
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- The top level node is a duplication node. However, human RBM22 is described in the literature (PMID:22246180) as being homologous to S. cerevisiae CWC2, even though it is in the other main subnode from the root.
- I have pruned the node Fungi_PTN001164531 because it either seems to misplaced within this tree or inappropriately placed in this tree at all.
-- In PMID:10207106, they indicate that human ROD1 is the homolog of S. pombe nrd1. Human ROD1 is present in PTHR11188:SF69 (PROTEIN ROD1-RELATED). This same paper characterizes S. pombe nrd1 as an RNA binding protein with a preference for poly(U).
-- There is some suggestion on the SGD page that S. cerevisiae MRN1 may be involved in translational regulation via binding to mRNAs, e.g. those that contain upstream uORFs.
-- There is no evidence that the characterized genes within this node are part of the PRP19 complex, while there is lots of good evidence for many of the genes in other places in this tree.
-- Although the MSA (in compressed view) doesn't look bad, I can't do anything sensible for these sequences because if I block the annotations coming from the root, it also blocks a reasonable annotation to "RNA binding" from being propagated even this more general annotation is made only at the level of this fungal node.
Annotation comments
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- Regarding human RBM22, the work in PMID:22246180 indicates that RBM22 is the human equivalent of yeast CWC2, and that it  binds U6 snRNA specifically (not just any snRNA), as well as pre-mRNA. Thus, I have propagated both "U6 snRNA binding" and "pre-mRNA binding" to the root node, even though it is a duplication node. I have also requested for the annotation to "snRNA binding" for human RBM22 to be updated to use the appropriate more specific term.
- The annotations to "poly(A) RNA binding for human RBM22 are from two large scale papers )PMID:22658674 & PMID:22681889). For many reasons I question the biological relevance of the specificity of these terms.

# REFERENCE
Annotation inferences using phylogenetic trees
The goal of the GO Reference Genome Project, described in PMID 19578431, is to provide accurate, complete and consistent GO annotations for all genes in twelve model organism genomes. To this end, GO curators are annotating evolutionary trees from the PANTHER database with GO terms describing molecular function, biological process and cellular component. GO terms based on experimental data from the scientific literature are used to annotate ancestral genes in the phylogenetic tree by sequence similarity (ISS), and unannotated descendants of these ancestral genes are inferred to have inherited these same GO annotations by descent. The annotations are done using a tool called PAINT (Phylogenetic Annotation and INference Tool).