# HISTORY 25 Mar 2016: Updated by: TOUCHUP-v1.15 15 Mar 2016: Updated by: TOUCHUP-v1.14 # molecular_function 20140912: node_PTN000236067 has function valine-tRNA ligase activity (GO:0004832) 20140911: Opisthokonta_PTN000236303 has function tyrosine-tRNA ligase activity (GO:0004831) 20140913: node_PTN000235772 has function isoleucine-tRNA ligase activity (GO:0004822) 20140913: cellular organisms_PTN000236210 has function leucine-tRNA ligase activity (GO:0004823) 20140911: Eukaryota_PTN000236416 has function leucine-tRNA ligase activity (GO:0004823) 20140911: Bacteria _PTN000236475 has function leucine-tRNA ligase activity (GO:0004823) 20140913: node_PTN000235847 has function methionine-tRNA ligase activity (GO:0004825) # cellular_component 20140912: root_PTN000235771 is found in cytosol (GO:0005829) 20140911: Eukaryota_PTN000235928 is found in mitochondrion (GO:0005739) 20140911: Eukaryota_PTN000236416 is found in mitochondrion (GO:0005739) 20140913: node_PTN000942566 is found in mitochondrion (GO:0005739) 20140911: Cyanobacteria_PTN000942434 is NOT found in mitochondrion (GO:0005739) 20140912: node_PTN000236067 is NOT found in cytosol (GO:0005829) 20140912: Eukaryota_PTN000236416 is NOT found in cytosol (GO:0005829) 20140912: node_PTN000942566 is NOT found in cytosol (GO:0005829) # biological_process 20140911: root_PTN000235771 participates in tRNA aminoacylation for protein translation (GO:0006418) 20140912: node_PTN000236067 participates in valyl-tRNA aminoacylation (GO:0006438) 20140911: Opisthokonta_PTN000236303 participates in tyrosyl-tRNA aminoacylation (GO:0006437) 20140913: node_PTN000235772 participates in isoleucyl-tRNA aminoacylation (GO:0006428) 20140911: cellular organisms_PTN000236210 participates in leucyl-tRNA aminoacylation (GO:0006429) 20140911: Eukaryota_PTN000236416 participates in leucyl-tRNA aminoacylation (GO:0006429) 20140911: Bacteria _PTN000236475 participates in leucyl-tRNA aminoacylation (GO:0006429) 20140913: node_PTN000235847 participates in methionyl-tRNA aminoacylation (GO:0006431) # WARNINGS - THE FOLLOWING HAVE BEEN REMOVED FOR THE REASONS NOTED # NOTES - Propagated 'cytokine activity' and 'inflammatory response' to PTN000236355 based on MSA (the similrity goes down in more distant organisms). TO DO - Annotate LUCA_PTN000942379 to "isoleucine-tRNA ligase activity" (added primary annotation to E. coli gene 2014-09-11). - Annotate LUCA_PTN000942633 to "ivaline-tRNA ligase activity" (added primary annotation to E. coli gene 2014-09-12). # REFERENCE Annotation inferences using phylogenetic trees The goal of the GO Reference Genome Project, described in PMID 19578431, is to provide accurate, complete and consistent GO annotations for all genes in twelve model organism genomes. To this end, GO curators are annotating evolutionary trees from the PANTHER database with GO terms describing molecular function, biological process and cellular component. GO terms based on experimental data from the scientific literature are used to annotate ancestral genes in the phylogenetic tree by sequence similarity (ISS), and unannotated descendants of these ancestral genes are inferred to have inherited these same GO annotations by descent. The annotations are done using a tool called PAINT (Phylogenetic Annotation and INference Tool).