# molecular_function 20160307: Magnoliophyta_PTN000189597 has function nitrate:proton symporter activity (GO:0009671) 20160307: root_PTN001363397 has function chloride ion binding (GO:0031404) 20160307: root_PTN001363397 has function voltage-gated chloride channel activity (GO:0005247) # cellular_component 20160307: Dikarya_PTN000914046 located in endoplasmic reticulum (GO:0005783) 20160307: Dikarya_PTN000914046 located in fungal-type vacuole (GO:0000324) 20160307: Dikarya_PTN000914046 located in Golgi apparatus (GO:0005794) 20160307: Dikarya_PTN000914046 located in endosome (GO:0005768) 20160307: Opisthokonta_PTN001706874 located in integral component of membrane (GO:0016021) 20160307: Opisthokonta_PTN001706874 located in endosome (GO:0005768) 20160307: Euteleostomi_PTN000189436 located in endosome (GO:0005768) 20160307: Opisthokonta_PTN000189255 located in integral component of plasma membrane (GO:0005887) 20160307: Metazoa_PTN001706894 located in lysosomal membrane (GO:0005765) 20160307: Magnoliophyta_PTN001363514 located in plant-type vacuole membrane (GO:0009705) 20160307: Magnoliophyta_PTN000189597 located in plant-type vacuole membrane (GO:0009705) 20160307: Magnoliophyta_PTN001363520 located in trans-Golgi network (GO:0005802) 20160307: Eukaryota_PTN001363449 located in lysosomal membrane (GO:0005765) 20160307: Bacteria _PTN000189633 located in plasma membrane (GO:0005886) 20160307: LUCA_PTN001706855 located in integral component of plasma membrane (GO:0005887) 20160307: Embryophyta_PTN000914191 located in chloroplast (GO:0009507) 20160307: Embryophyta_PTN000914191 located in Golgi apparatus (GO:0005794) 20160307: Eukaryota_PTN000189254 located in integral component of plasma membrane (GO:0005887) # biological_process 20160307: Magnoliophyta_PTN000189597 participates in nitrate transport (GO:0015706) 20160307: Dikarya_PTN000914046 participates in cellular iron ion homeostasis (GO:0006879) 20160307: Dikarya_PTN000914046 participates in cellular copper ion homeostasis (GO:0006878) 20160307: Opisthokonta_PTN001706874 participates in endosomal lumen acidification (GO:0048388) 20160307: LUCA_PTN001706855 participates in nitrate transport (GO:0015706) 20160307: root_PTN001363397 participates in chloride transmembrane transport (GO:1902476) # Notes Annotation inferences using phylogenetic trees The goal of the GO Reference Genome Project, described in PMID 19578431, is to provide accurate, complete and consistent GO annotations for all genes in twelve model organism genomes. To this end, GO curators are annotating evolutionary trees from the PANTHER database with GO terms describing molecular function, biological process and cellular component. GO terms based on experimental data from the scientific literature are used to annotate ancestral genes in the phylogenetic tree by sequence similarity (ISS), and unannotated descendants of these ancestral genes are inferred to have inherited these same GO annotations by descent. The annotations are done using a tool called PAINT (Phylogenetic Annotation and INference Tool).