# HISTORY 24 Mar 2016: Updated by: TOUCHUP-v1.15 14 Mar 2016: Updated by: TOUCHUP-v1.14 # molecular_function 20120409: root_PTN000119716 has function GTPase activity (GO:0003924) # cellular_component 20120409: node_PTN000120057 is found in mitochondrion (GO:0005739) 20120409: Eukaryota_PTN000119850 is found in cytosol (GO:0005829) 20120409: Eukaryota_PTN000119781 is found in nucleolus (GO:0005730) 20120409: Eukaryota_PTN000119956 is found in nucleolus (GO:0005730) 20120409: Eukaryota_PTN000119720 is found in mitochondrion (GO:0005739) 20120409: Bacteria _PTN000744586 is NOT found in mitochondrion (GO:0005739) 20120409: Bacilli_PTN001670152 is NOT found in mitochondrion (GO:0005739) # biological_process 20120409: root_PTN000119716 participates in ribosome biogenesis (GO:0042254) # PRUNED 24 Mar 2016: Bacteria _PTN000120104 has been pruned from tree # WARNINGS - THE FOLLOWING HAVE BEEN REMOVED FOR THE REASONS NOTED # NOTES 14 Mar 2016: Bacteria _PTN000120104 has been pruned from tree Fast-pass curation of PTHR11089, the GTP-BINDING PROTEIN-RELATED family. Review and integration of known information into a phylogenetic model can be found in PMID 16209721. Our phylogeny is very consistent overall. The experimental annotations are also consistent with the model proposed in the paper, which we largely replicate here: GTPase activity (MF) and ribosome biogenesis (BP) were present at the root of the entire family, with specific location (CC) annotations in different eukaryotic clades consistent with conservation since gene duplication. The clade that includes scpB (BACSU) was pruned from the family, since it lacks conserved patterns found in all the other sequences. # REFERENCE Annotation inferences using phylogenetic trees The goal of the GO Reference Genome Project, described in PMID 19578431, is to provide accurate, complete and consistent GO annotations for all genes in twelve model organism genomes. To this end, GO curators are annotating evolutionary trees from the PANTHER database with GO terms describing molecular function, biological process and cellular component. GO terms based on experimental data from the scientific literature are used to annotate ancestral genes in the phylogenetic tree by sequence similarity (ISS), and unannotated descendants of these ancestral genes are inferred to have inherited these same GO annotations by descent. The annotations are done using a tool called PAINT (Phylogenetic Annotation and INference Tool).