CDC6 association with ORC:origin complexes mediated by MCM8
The geminin component of geminin:Cdt1 complexes is ubiquitinated, releasing Cdt1
Ubiquitinated geminin is degraded by the proteasome
Free CDT1 associates with CDC6:ORC:origin complexes
Mcm2-7 associates with the Cdt1:CDC6:ORC:origin complex, forming the pre-replicative complex (preRC)
Orc3 associates with Orc2 constitutively bound at origins of replication
Orc5 associates with Orc3:Orc2:origin complexes
Orc4 associates with Orc5:Orc3:Orc2:origin complexes
Orc1 associates with Orc4:Orc5:Orc3:Orc2:origin complexes
Orc6 associates with Orc1:Orc4:Orc5:Orc3:Orc2:origin complexes, forming ORC:origin complexes
Association of MCM8 with ORC:origin complex
Assembly of the pre-replicative complex
Assembly of the ORC complex at the origin of replication
CDC6 association with the ORC:origin complex
CDT1 association with the CDC6:ORC:origin complex
CDC6 protein is synthesized under the control of E2F transcription factors