Mcm10 associates with the pre-replicative complex, stabilizing Mcm2-7
Cdt1 is displaced from the pre-replicative complex.
CDK and DDK associate with the Mcm10:pre-replicative complex
Mcm2-7 is phosphorylated by DDK
Cdc45 associates with the pre-replicative complex at the origin
DNA Replication Factor A (RPA) associates with the pre-replicative complex at the origin
DNA polymerase alpha:primase binds at the origin
DNA polymerase epsilon binds at the origin
Activation of the pre-replicative complex