<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="TrEMBL" created="2016-11-02" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>A0A1B0UHX3</accession>
    <name>A0A1B0UHX3_9ADEN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protease</fullName>
            <ecNumber evidence="6">3.4.22.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Adenain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Adenovirus protease</fullName>
            <shortName evidence="6">AVP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Adenovirus proteinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Endoprotease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">L3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Bat mastadenovirus WIV13</name>
        <dbReference type="NCBI Taxonomy" id="1788435"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Rowavirales</taxon>
            <taxon>Adenoviridae</taxon>
            <taxon>Mastadenovirus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2015-08" db="EMBL/GenBank/DDBJ databases">
            <title>Isolation and characterization of novel bat adenoviruses with diverse genome sizes, low GC contents or extremely long E3 ORFs.</title>
            <authorList>
                <person name="Tan B."/>
                <person name="Yang X.-L."/>
                <person name="Ge X.-Y."/>
                <person name="Peng C."/>
                <person name="Zhang Y.-Z."/>
                <person name="Zhang L.-B."/>
                <person name="Shi Z.-L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">WIV13</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Cleaves viral precursor proteins (pTP, pIIIa, pVI, pVII, pVIII, and pX) inside newly assembled particles giving rise to mature virions. Protease complexed to its cofactor slides along the viral DNA to specifically locate and cleave the viral precursors. Mature virions have a weakened organization compared to the unmature virions, thereby facilitating subsequent uncoating. Without maturation, the particle lacks infectivity and is unable to uncoat. Late in adenovirus infection, in the cytoplasm, may participate in the cytoskeleton destruction. Cleaves host cell cytoskeletal keratins K7 and K18.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).</text>
            <dbReference type="EC" id="3.4.22.39"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Requires DNA and protease cofactor for maximal activation. Inside nascent virions, becomes partially activated by binding to the viral DNA, allowing it to cleave the cofactor that binds to the protease and fully activates it. Actin, like the viral protease cofactor, seems to act as a cofactor in the cleavage of cytokeratin 18 and of actin itself.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with protease cofactor pVI-C; this interaction is necessary for protease activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Host nucleus</location>
        </subcellularLocation>
        <text evidence="6">Present in about 10 copies per virion.</text>
    </comment>
    <comment type="induction">
        <text evidence="6">Expressed in the late phase of the viral replicative cycle.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the peptidase C5 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.39" evidence="6"/>
    <dbReference type="EMBL" id="KT698852">
        <property type="protein sequence ID" value="AMB43032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_009272932.1">
        <property type="nucleotide sequence ID" value="NC_030874.1"/>
    </dbReference>
    <dbReference type="GeneID" id="28715677"/>
    <dbReference type="KEGG" id="vg:28715677"/>
    <dbReference type="Proteomes" id="UP000108481">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044423">
        <property type="term" value="C:virion component"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04059">
        <property type="entry name" value="ADV_PRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000855">
        <property type="entry name" value="Peptidase_C5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00770">
        <property type="entry name" value="Peptidase_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001218">
        <property type="entry name" value="Protease_ADV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00703">
        <property type="entry name" value="ADVENDOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 6 7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 6" id="KW-1048">Host nucleus</keyword>
    <keyword evidence="6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0426">Late protein</keyword>
    <keyword evidence="6 7 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3 6" id="KW-0946">Virion</keyword>
    <feature type="active site" evidence="6 8">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="active site" evidence="6 8">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" evidence="6 8">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="6">
        <location>
            <begin position="51"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (with C-10 in cleaved protease cofactor pVI-C)" evidence="6">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001218-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AMB43032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000108481"/>
        </source>
    </evidence>
    <sequence length="202" mass="23362" checksum="59EB59E87218C8C4" modified="2016-11-02" version="1">MGSSETELKHIITDLGVGKYFLGTFDKRFPGFINKDKPCCAIVNTAFRETGGMHWIAFAWYPPSFTFYMFDPFGFSDEKLKQIYNFEYKSLLKRSAITSGINKCVTFIKSTESVQGGHSAACGLFCCAFLYSFVNYPLNPMKNKFMDIFKSVPNNKILEPQCQYIFKKNQENLYTFLLLNSPYFKSHENQIKCQTKFDKLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-11-02" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>A0A1B0UHY7</accession>
    <name>A0A1B0UHY7_9ADEN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pre-hexon-linking protein VIII</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pre-protein VIII</fullName>
            <shortName evidence="1">pVIII</shortName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="1">Hexon-linking protein-N</fullName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">12.1 kDa protein VIII</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">Protein VIII-N</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Hexon-linking protein-C</fullName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">7.6 kDa protein VIII</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">Protein VIII-C</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">L4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Bat mastadenovirus WIV13</name>
        <dbReference type="NCBI Taxonomy" id="1788435"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Rowavirales</taxon>
            <taxon>Adenoviridae</taxon>
            <taxon>Mastadenovirus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2015-08" db="EMBL/GenBank/DDBJ databases">
            <title>Isolation and characterization of novel bat adenoviruses with diverse genome sizes, low GC contents or extremely long E3 ORFs.</title>
            <authorList>
                <person name="Tan B."/>
                <person name="Yang X.-L."/>
                <person name="Ge X.-Y."/>
                <person name="Peng C."/>
                <person name="Zhang Y.-Z."/>
                <person name="Zhang L.-B."/>
                <person name="Shi Z.-L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">WIV13</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Hexon-linking protein-C: Structural component of the virion that acts as a cement protein on the capsid interior and which glue the peripentonal hexons and group-of-nine hexons together.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Hexon-linking protein-N: Structural component of the virion that acts as a cement protein on the capsid interior and which glue the peripentonal hexons and group-of-nine hexons together.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with the peripentonal hexons as well as the hexons in the facets. Part of a complex composed of the core-capsid bridging protein, the endosome lysis protein VI and the hexon-linking protein VIII; these interactions bridge the virus core to the capsid.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Hexon-linking protein-N</molecule>
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <text evidence="1">Located on the inner side of the capsid shell. Present in 120 copies per virion.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Hexon-linking protein-C</molecule>
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <text evidence="1">Located on the inner side of the capsid shell. Present in 120 copies per virion.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Pre-hexon-linking protein VIII</molecule>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="1">Expressed in the late phase of the viral replicative cycle.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Cleaved by the viral protease during virion maturation. May cause the middle segment to be shed from the capsid.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenoviridae hexon-linking protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KT698852">
        <property type="protein sequence ID" value="AMB43037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_009272937.1">
        <property type="nucleotide sequence ID" value="NC_030874.1"/>
    </dbReference>
    <dbReference type="GeneID" id="28715682"/>
    <dbReference type="KEGG" id="vg:28715682"/>
    <dbReference type="Proteomes" id="UP000108481">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019028">
        <property type="term" value="C:viral capsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031423">
        <property type="term" value="F:hexon binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04049">
        <property type="entry name" value="ADV_CAP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000646">
        <property type="entry name" value="Adeno_PVIII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01310">
        <property type="entry name" value="Adeno_PVIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0167">Capsid protein</keyword>
    <keyword evidence="1" id="KW-1048">Host nucleus</keyword>
    <keyword evidence="1" id="KW-0426">Late protein</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_5023531153" description="Pre-hexon-linking protein VIII" evidence="1">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="peptide" id="PRO_5011803885" description="Hexon-linking protein-N" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="peptide" id="PRO_5011803884" description="Hexon-linking protein-C" evidence="1">
        <location>
            <begin position="141"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="1">
        <location>
            <begin position="111"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="1">
        <location>
            <begin position="140"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine; by host" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AMB43037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000108481"/>
        </source>
    </evidence>
    <sequence length="210" mass="23058" checksum="92A85B5A60B112F9" modified="2016-11-02" version="1">MSKEIPTPYVWTFQPQMGVAAGASQDYSTQMNWLSAGNNMSQKVFALRNQRNNILEKQAIITETPRLLPNPPRWPARKIDHSLKIPTLVNLPRNQFLETYMSNSGMQLAGGGITGGSIQLNNEYVGMPIRSDGVMQLAGGGSHSLTPLNAYLLLQSASSMPRNGGIGSNQFVKEFVPTVYYNPYSSSPASFPDQFISNYDVISDSVAEYS</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>