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<entry dataset="Swiss-Prot" created="1988-01-01" modified="2022-02-23" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>P06945</accession>
    <name>VP2_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>Outer capsid protein VP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">Segment-2</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1985" name="J. Virol." volume="55" first="826" last="830">
            <title>Complete sequence of bluetongue virus L2 RNA that codes for the antigen recognized by neutralizing antibodies.</title>
            <authorList>
                <person name="Purdy M.A."/>
                <person name="Ghiasi H."/>
                <person name="Rao C.D."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="2991598"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">The VP2 protein is one of the two proteins (with VP5) which constitute the virus particle outer capsid. It is the major target of the host immunogenic response. Responsible for viral attachment to target host cell, probably by binding to sialic acid. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the orbivirus VP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="M11787">
        <property type="protein sequence ID" value="AAA42828.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="A25484">
        <property type="entry name" value="P2XR10"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_052958.1">
        <property type="nucleotide sequence ID" value="NC_006013.1"/>
    </dbReference>
    <dbReference type="SMR" id="P06945"/>
    <dbReference type="GeneID" id="2943152"/>
    <dbReference type="KEGG" id="vg:2943152"/>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001742">
        <property type="entry name" value="Capsid_VP2_Orbivir"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00898">
        <property type="entry name" value="Orbi_VP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1165">Clathrin-mediated endocytosis of virus by host</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1153">Inner capsid protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1164">Virus endocytosis by host</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="chain" id="PRO_0000222684" description="Outer capsid protein VP2">
        <location>
            <begin position="1"/>
            <end position="956"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="956" mass="111022" checksum="2BDA261973724810" modified="1988-01-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1988-04-01" modified="2020-12-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>P07131</accession>
    <name>VNS1_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>Non-structural protein NS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">Segment-5</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1987" name="Nucleic Acids Res." volume="15" first="7207" last="7207">
            <title>Complete sequence of the NS1 gene (M6 RNA) of US bluetongue virus serotype 10.</title>
            <authorList>
                <person name="Lee J."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="2821505"/>
            <dbReference type="DOI" id="10.1093/nar/15.17.7207"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the orbivirus non-structural protein NS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y00422">
        <property type="protein sequence ID" value="CAA68484.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="PIR" id="A26863">
        <property type="entry name" value="MNXRS1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_052970.1">
        <property type="nucleotide sequence ID" value="NC_006025.1"/>
    </dbReference>
    <dbReference type="SMR" id="P07131"/>
    <dbReference type="GeneID" id="2943158"/>
    <dbReference type="KEGG" id="vg:2943158"/>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002630">
        <property type="entry name" value="Orbi_NS1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01718">
        <property type="entry name" value="Orbi_NS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000222665" description="Non-structural protein NS1">
        <location>
            <begin position="1"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="552" mass="64445" checksum="CE525680592D5C73" modified="1988-04-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1988-04-01" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>P07132</accession>
    <name>VP4_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>Core protein VP4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">Segment-4</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1987" name="Nucleic Acids Res." volume="15" first="7206" last="7206">
            <title>Nucleotide sequence of the VP4 core protein gene (M4 RNA) of US bluetongue virus serotype 10.</title>
            <authorList>
                <person name="Yu Y."/>
                <person name="Fukusho A."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="2821504"/>
            <dbReference type="DOI" id="10.1093/nar/15.17.7206"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2006-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Roy P."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <comment type="function">
        <text>The VP4 protein is one of the five proteins (with VP1, VP3, VP6 and VP7) which form the inner capsid of the virus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the orbivirus VP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y00421">
        <property type="protein sequence ID" value="CAA68483.2"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="PIR" id="A26862">
        <property type="entry name" value="P4XRBV"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_052969.2">
        <property type="nucleotide sequence ID" value="NC_006024.2"/>
    </dbReference>
    <dbReference type="PDB" id="2JH8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.22 A"/>
        <property type="chains" value="A=1-644"/>
    </dbReference>
    <dbReference type="PDB" id="2JH9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A=1-644"/>
    </dbReference>
    <dbReference type="PDB" id="2JHA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="A=1-644"/>
    </dbReference>
    <dbReference type="PDB" id="2JHC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A=1-644"/>
    </dbReference>
    <dbReference type="PDB" id="2JHP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A=1-644"/>
    </dbReference>
    <dbReference type="PDBsum" id="2JH8"/>
    <dbReference type="PDBsum" id="2JH9"/>
    <dbReference type="PDBsum" id="2JHA"/>
    <dbReference type="PDBsum" id="2JHC"/>
    <dbReference type="PDBsum" id="2JHP"/>
    <dbReference type="SMR" id="P07132"/>
    <dbReference type="GeneID" id="2943157"/>
    <dbReference type="KEGG" id="vg:2943157"/>
    <dbReference type="EvolutionaryTrace" id="P07132"/>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039624">
        <property type="term" value="C:viral outer capsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20758">
        <property type="entry name" value="capping_2-OMTase_Orbivirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007753">
        <property type="entry name" value="Orbi_VP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043026">
        <property type="entry name" value="Orbi_VP4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05059">
        <property type="entry name" value="Orbi_VP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1152">Outer capsid protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000222705" description="Core protein VP4">
        <location>
            <begin position="1"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="7"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="12"/>
            <end position="14"/>
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    <feature type="helix" evidence="4">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
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        <location>
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    <feature type="helix" evidence="4">
        <location>
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        <location>
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        </location>
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    <feature type="strand" evidence="4">
        <location>
            <begin position="79"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
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    <feature type="turn" evidence="4">
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    <feature type="strand" evidence="2">
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            <end position="517"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="568"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="579"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="584"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="587"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="612"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="627"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="635"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2JH9"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2JHA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2JHP"/>
        </source>
    </evidence>
    <sequence length="644" mass="75385" checksum="D056FB588006E150" modified="2007-01-23" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1988-04-01" modified="2020-12-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>P07389</accession>
    <name>VP5_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>Outer capsid protein VP5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">Segment-6</name>
        <name type="synonym">M5</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1986" name="J. Gen. Virol." volume="67" first="957" last="962">
            <title>Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10.</title>
            <authorList>
                <person name="Purdy M.A."/>
                <person name="Ritter G.D."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="3009695"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Proc. Natl. Acad. Sci. U.S.A." volume="101" first="2100" last="2105">
            <title>A capsid protein of nonenveloped Bluetongue virus exhibits membrane Permeabilization activity.</title>
            <authorList>
                <person name="Forzan M."/>
                <person name="Wirblich C."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="14762165"/>
            <dbReference type="DOI" id="10.1073/pnas.0306448101"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">VP5 protein is one of the two proteins (with VP2) which constitute the virus particle outer capsid. Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. Permeabilization activity is probably negatively regulated by VP2 and is triggered by endosomal degradation of VP2 and exposure to low pH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the orbivirus VP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="D12532">
        <property type="protein sequence ID" value="BAA02095.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="A25419">
        <property type="entry name" value="P5XR10"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_052955.1">
        <property type="nucleotide sequence ID" value="NC_006010.1"/>
    </dbReference>
    <dbReference type="SMR" id="P07389"/>
    <dbReference type="GeneID" id="2943151"/>
    <dbReference type="KEGG" id="vg:2943151"/>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000145">
        <property type="entry name" value="Capsid_VP5_Orbivir"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00901">
        <property type="entry name" value="Orbi_VP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1152">Outer capsid protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-1173">Viral penetration via permeabilization of host membrane</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="chain" id="PRO_0000222712" description="Outer capsid protein VP5">
        <location>
            <begin position="1"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in membrane permeabilization">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="14762165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="526" mass="59163" checksum="29AE556DBD55522C" modified="1988-04-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1988-08-01" modified="2020-08-12" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>P08363</accession>
    <name>VP8_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>Non-structural protein P8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Non-structural protein NS3</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Non-structural protein NS3A</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">Segment-10</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1986" name="J. Gen. Virol." volume="67" first="2833" last="2837">
            <title>Nucleotide sequence of a cDNA clone of RNA segment 10 of bluetongue virus (serotype 10).</title>
            <authorList>
                <person name="Lee J.W."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="3025349"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1991" name="J. Gen. Virol." volume="72" first="2263" last="2267">
            <title>Localization of the non-structural protein NS3 in bluetongue virus-infected cells.</title>
            <authorList>
                <person name="Hyatt A.D."/>
                <person name="Gould A.R."/>
                <person name="Coupar B."/>
                <person name="Eaton B.T."/>
            </authorList>
            <dbReference type="PubMed" id="1654377"/>
            <dbReference type="DOI" id="10.1099/0022-1317-72-9-2263"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1993" name="Virology" volume="193" first="592" last="603">
            <title>Release of bluetongue virus-like particles from insect cells is mediated by BTV nonstructural protein NS3/NS3A.</title>
            <authorList>
                <person name="Hyatt A.D."/>
                <person name="Zhao Y."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="8384747"/>
            <dbReference type="DOI" id="10.1006/viro.1993.1167"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="43092" last="43097">
            <title>The NS3 protein of bluetongue virus exhibits viroporin-like properties.</title>
            <authorList>
                <person name="Han Z."/>
                <person name="Harty R.N."/>
            </authorList>
            <dbReference type="PubMed" id="15292261"/>
            <dbReference type="DOI" id="10.1074/jbc.M403663200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2013" name="J. Virol." volume="87" first="8241" last="8246">
            <title>NS3 of bluetongue virus interferes with the induction of type I interferon.</title>
            <authorList>
                <person name="Chauveau E."/>
                <person name="Doceul V."/>
                <person name="Lara E."/>
                <person name="Breard E."/>
                <person name="Sailleau C."/>
                <person name="Vidalain P.O."/>
                <person name="Meurs E.F."/>
                <person name="Dabo S."/>
                <person name="Schwartz-Cornil I."/>
                <person name="Zientara S."/>
                <person name="Vitour D."/>
            </authorList>
            <dbReference type="PubMed" id="23658442"/>
            <dbReference type="DOI" id="10.1128/JVI.00678-13"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2015" name="Virus Genes" volume="51" first="244" last="251">
            <title>A coiled-coil motif in non-structural protein 3 (NS3) of bluetongue virus forms an oligomer.</title>
            <authorList>
                <person name="Chacko N."/>
                <person name="Mohanty N.N."/>
                <person name="Biswas S.K."/>
                <person name="Chand K."/>
                <person name="Yogisharadhya R."/>
                <person name="Pandey A.B."/>
                <person name="Mondal B."/>
                <person name="Shivachandra S.B."/>
            </authorList>
            <dbReference type="PubMed" id="26318174"/>
            <dbReference type="DOI" id="10.1007/s11262-015-1230-9"/>
        </citation>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2016" name="BMC Biol." volume="14" first="69" last="69">
            <title>The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing.</title>
            <authorList>
                <person name="Pourcelot M."/>
                <person name="Zemirli N."/>
                <person name="Silva Da Costa L."/>
                <person name="Loyant R."/>
                <person name="Garcin D."/>
                <person name="Vitour D."/>
                <person name="Munitic I."/>
                <person name="Vazquez A."/>
                <person name="Arnoult D."/>
            </authorList>
            <dbReference type="PubMed" id="27538435"/>
            <dbReference type="DOI" id="10.1186/s12915-016-0292-z"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INTERACTION WITH HOST OPTN</scope>
    </reference>
    <comment type="function">
        <text evidence="4 6">Plays a role in the inhibition of host innate immune response. Interacts with host OPTN and thus inhibits the recruitment of TBK1 to the host Golgi apparatus. In turn, downstream partner IRF3 cannot be activated and IFN-beta production is impaired.</text>
    </comment>
    <comment type="function">
        <text evidence="2 7">Facilitates viral particle release either by increasing plasma membrane permeability through a viroporin-like activity (PubMed:15292261) or by viral budding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5 6">Forms homooligomers via coiled-coil motif (PubMed:15292261, PubMed:26318174). Interacts with host OPTN; this interaction inhibits innate immune response (PubMed:27538435).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 3">Host cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 6">Host Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the orbivirus NS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="M28981">
        <property type="protein sequence ID" value="AAA42840.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="A29153">
        <property type="entry name" value="P8XR10"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_052960.1">
        <property type="nucleotide sequence ID" value="NC_006015.1"/>
    </dbReference>
    <dbReference type="ELM" id="P08363"/>
    <dbReference type="GeneID" id="2943154"/>
    <dbReference type="KEGG" id="vg:2943154"/>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002565">
        <property type="entry name" value="Orbi_NS3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01616">
        <property type="entry name" value="Orbi_NS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="chain" id="PRO_0000040628" description="Non-structural protein P8">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000040629" description="Non-structural protein NS3A">
        <location>
            <begin position="14"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="CCM-I" evidence="5">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="CCM-III" evidence="5">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="CCM-II" evidence="5">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15292261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="1654377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="23658442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="26318174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="27538435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="8384747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="8"/>
    <sequence length="229" mass="25602" checksum="1A23B53198ECB684" modified="1988-08-01" version="1">MLSGLIQRFEEEKMKHNQDRVEELSLVRVDDTISQPPRYAPSAPMPSSMPTVALEILDKAMSNTTGATQTQKAEKAAFASYAEAFRDDVRLRQIKRHVNEQILPKLKSDLSELKKKRAIIHTTLLVAAVVALLTSVCTLSSDMSVAFKINGTKTEVPSWFKSLNPMLGVVNLGATFLMMVCAKSERALNQQIDMIKKEVMKKQSYNDAVRMSFTEFSSIPLDGFEMPLT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1989-10-01" modified="2022-02-23" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>P12435</accession>
    <name>VP3_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>Core protein VP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Major inner capsid protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">Segment-3</name>
        <name type="synonym">L3</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1985" name="Virus Res." volume="3" first="181" last="190">
            <title>The complete sequence of bluetongue virus serotype 10 segment 3 and its predicted VP3 polypeptide compared with those of BTV serotype 17.</title>
            <authorList>
                <person name="Ghiasi H."/>
                <person name="Purdy M.A."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="2998117"/>
            <dbReference type="DOI" id="10.1016/0168-1702(85)90007-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text>The VP3 protein is one of the five proteins (with VP1, VP4, VP6 and VP7) which form the inner capsid of the virus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the orbivirus VP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="M22096">
        <property type="protein sequence ID" value="AAA42832.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="S07419">
        <property type="entry name" value="S07419"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_052959.1">
        <property type="nucleotide sequence ID" value="NC_006014.1"/>
    </dbReference>
    <dbReference type="SMR" id="P12435"/>
    <dbReference type="GeneID" id="2943153"/>
    <dbReference type="KEGG" id="vg:2943153"/>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002614">
        <property type="entry name" value="Inner_layer_core_VP3_Orbivir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016029">
        <property type="entry name" value="Inner_layer_core_VP3_Reovir"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01700">
        <property type="entry name" value="Orbi_VP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56831">
        <property type="entry name" value="SSF56831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000222695" description="Core protein VP3">
        <location>
            <begin position="1"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="901" mass="103343" checksum="4E485F5A38FC6456" modified="1989-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-01-01" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>P13840</accession>
    <name>RDRP_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>RNA-directed RNA polymerase</fullName>
            <ecNumber>2.7.7.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>VP1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">Segment-1</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1988" name="Nucleic Acids Res." volume="16" first="11759" last="11767">
            <title>Evidence for genetic relationship between RNA and DNA viruses from the sequence homology of a putative polymerase gene of bluetongue virus with that of vaccinia virus: conservation of RNA polymerase genes from diverse species.</title>
            <authorList>
                <person name="Roy P."/>
                <person name="Fukusho A."/>
                <person name="Ritter G.D."/>
                <person name="Lyon D."/>
            </authorList>
            <dbReference type="PubMed" id="2850542"/>
            <dbReference type="DOI" id="10.1093/nar/16.24.11759"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the reoviridae RNA-directed RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48"/>
    <dbReference type="EMBL" id="X12819">
        <property type="protein sequence ID" value="CAA31306.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="A34296">
        <property type="entry name" value="RRXRBT"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_052968.1">
        <property type="nucleotide sequence ID" value="NC_006023.1"/>
    </dbReference>
    <dbReference type="SMR" id="P13840"/>
    <dbReference type="GeneID" id="2943156"/>
    <dbReference type="KEGG" id="vg:2943156"/>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007097">
        <property type="entry name" value="RNA-dir_pol_reovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008723">
        <property type="entry name" value="RNA_pol_orbivir"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05788">
        <property type="entry name" value="Orbi_VP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000821">
        <property type="entry name" value="RdRPol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50523">
        <property type="entry name" value="RDRP_DSRNA_REO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0693">Viral RNA replication</keyword>
    <feature type="chain" id="PRO_0000222660" description="RNA-directed RNA polymerase">
        <location>
            <begin position="1"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="domain" description="RdRp catalytic" evidence="1">
        <location>
            <begin position="562"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="1302" mass="149587" checksum="0C063AE396F0C33D" modified="1990-01-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1991-11-01" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>P23065</accession>
    <name>VNS2_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>Non-structural protein NS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">Segment-8</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1989" name="J. Gen. Virol." volume="70" first="1677" last="1689">
            <title>Completion of the sequence of bluetongue virus serotype 10 by the characterization of a structural protein, VP6, and a non-structural protein, NS2.</title>
            <authorList>
                <person name="Fukusho A."/>
                <person name="Yu Y."/>
                <person name="Yamaguchi S."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="2544660"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text>Single-stranded RNA-binding protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the orbivirus non-structural protein NS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="D00500">
        <property type="protein sequence ID" value="BAA00392.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="A32400">
        <property type="entry name" value="MNXRC3"/>
    </dbReference>
    <dbReference type="PDB" id="1UTY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-177"/>
    </dbReference>
    <dbReference type="PDBsum" id="1UTY"/>
    <dbReference type="SMR" id="P23065"/>
    <dbReference type="EvolutionaryTrace" id="P23065"/>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007602">
        <property type="entry name" value="BTV_NS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037194">
        <property type="entry name" value="NS2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04514">
        <property type="entry name" value="BTV_NS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110132">
        <property type="entry name" value="SSF110132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000222675" description="Non-structural protein NS2">
        <location>
            <begin position="1"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="22"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="44"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="56"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="73"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="84"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="97"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="102"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="138"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="154"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1UTY"/>
        </source>
    </evidence>
    <sequence length="357" mass="40999" checksum="3484FD802F9327A1" modified="1991-11-01" version="1">MEQKQRRFTKNIFVLDVTAKTLCGAIAKLSSQPYCQIKIGRVVAFKPVKNPEPKGYVLNVPGPGAYRIQDGQDIISLMLTPHGVEATTERWEEWKFEGVSVTPMATRVQYNGVMVDAEIKYCKGMGIVQPYMRNDFDRNEMPDLPGVMRSNYDIRELRQKIKNERESAPRLQVHSVAPREESRWMDDDEAKVDDEAKEIVPGTSGLEKLREARSNVFKEVEAVINWNLDERDEGDRDERGDEEQVKTLSDDDDQGEDASDDEHPKTHITKEYIEKVAKQIKLKDERFMSLSSAMPQASGGFDRMIVTKKLKWQNVPLYCFDESLKRYELQCVGACERVAFVSKDMSLIICRSAFRRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-02-01" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>P33423</accession>
    <name>VP6B_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>Protein VP6-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Minor inner core protein VP6-B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">Segment-9</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1992" name="Virus Res." volume="24" first="315" last="323">
            <title>Comparative sequence analyses of the cognate structural protein VP6 genes of five US bluetongue viruses.</title>
            <authorList>
                <person name="Hwang G.-Y."/>
                <person name="Chiou J.-F."/>
                <person name="Yang Y.-Y."/>
                <person name="Li J.K.-K."/>
            </authorList>
            <dbReference type="PubMed" id="1329371"/>
            <dbReference type="DOI" id="10.1016/0168-1702(92)90126-T"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text>Surrounds and interacts with the genomic dsRNA. Possesses ss- and dsRNA-binding capacity. Its hydrophilic nature and capability to bind ss- and dsRNA suggest that it interacts with BTV genomic RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <text>Inner capsid.</text>
    </comment>
    <comment type="alternative products">
        <event type="alternative initiation"/>
        <isoform>
            <id>P33423-1</id>
            <name>Protein VP6-B</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q98829-1</id>
            <name>Protein VP6-A</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the orbivirus VP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="L08669">
        <property type="protein sequence ID" value="AAA42818.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="B48561">
        <property type="entry name" value="B48561"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001399">
        <property type="entry name" value="Orbi_VP6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01516">
        <property type="entry name" value="Orbi_VP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00902">
        <property type="entry name" value="VP6CAPSID"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0024">Alternative initiation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000222723" description="Protein VP6-B">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="325" mass="35163" checksum="AC759B7005DACD5A" modified="1994-02-01" version="1">MLLAPGDVIKRSSEELKQRQIQINLIDWIESESGKEDKAEPKEESKAEESKDGQGTQSESSQKKEGSKEAKDADVDRRIHTAVGSGSSAKGPGVRANENVDRGDGKVGGGGGNADAGVGTIGANGGRWVVLTEEIARAIESKYGTKIDVYRDKVPAQIIEVERSLQKELGISREGVAEQTERLRDLRRKEKSGAHAKAAERGRRKQGKKPHGDVQKEGTEEEKTSEEQASVGIAIEGVMSQKKLLSMIGGVERKMAPIGARESAVMLVSNSIKDVVRATAYFTAPTGDPHWKEVAREASKKKNILAYTSTGGDVKTEFLHLIDHL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1988-08-01" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>P69361</accession>
    <accession>P07886</accession>
    <name>VP7_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>Core protein VP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">Segment-7</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1988" name="Nucleic Acids Res." volume="16" first="1620" last="1620">
            <title>Complete nucleotide sequence of the group-reactive antigen VP7 gene of bluetongue virus.</title>
            <authorList>
                <person name="Yu Y."/>
                <person name="Fukusho A."/>
                <person name="Ritter D.G."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="2831505"/>
            <dbReference type="DOI" id="10.1093/nar/16.4.1620"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1995" name="Nature" volume="373" first="167" last="170">
            <title>The crystal structure of bluetongue virus VP7.</title>
            <authorList>
                <person name="Grimes J."/>
                <person name="Basak A.K."/>
                <person name="Roy P."/>
                <person name="Stuart D."/>
            </authorList>
            <dbReference type="PubMed" id="7816101"/>
            <dbReference type="DOI" id="10.1038/373167a0"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text>The VP7 protein is one of the five proteins (with VP1, VP3, VP4, and VP6) which form the inner capsid of the virus.</text>
    </comment>
    <comment type="subunit">
        <text>Homotrimer that assemble in a complex of 260 capsomers on an inner scaffold composed of VP3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the orbivirus VP7 family.</text>
    </comment>
    <dbReference type="EMBL" id="X06463">
        <property type="protein sequence ID" value="CAA29771.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="A28537">
        <property type="entry name" value="P7XR10"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_052967.1">
        <property type="nucleotide sequence ID" value="NC_006022.1"/>
    </dbReference>
    <dbReference type="PDB" id="1BVP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="1/2/3/4/5/6=1-349"/>
    </dbReference>
    <dbReference type="PDBsum" id="1BVP"/>
    <dbReference type="SMR" id="P69361"/>
    <dbReference type="GeneID" id="2943155"/>
    <dbReference type="KEGG" id="vg:2943155"/>
    <dbReference type="EvolutionaryTrace" id="P69361"/>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008980">
        <property type="entry name" value="Capsid_hemagglutn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001803">
        <property type="entry name" value="Orbi_VP7_capsid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023178">
        <property type="entry name" value="Orbi_VP7_capsid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023176">
        <property type="entry name" value="Orbi_VP7_capsid_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008935">
        <property type="entry name" value="Virus_capsid_a-hlx_vir"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00897">
        <property type="entry name" value="Orbi_VP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00903">
        <property type="entry name" value="VP7CAPSID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48345">
        <property type="entry name" value="SSF48345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49818">
        <property type="entry name" value="SSF49818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1152">Outer capsid protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000222732" description="Core protein VP7">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="2"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="90"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="148"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="158"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="168"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="175"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="201"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="209"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="215"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="223"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="227"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="231"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="237"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="263"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="289"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="293"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="341"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1BVP"/>
        </source>
    </evidence>
    <sequence length="349" mass="38548" checksum="F08EDD6DD2CE86D9" modified="1988-08-01" version="1">MDTIAARALTVMRACATLQEARIVLEANVMEILGIAINRYNGLTLRGVTMRPTSLAQRNEMFFMCLDMMLSAAGINVGPISPDYTQHMATIGVLATPEIPFTTEAANEIARVTGETSTWGPARQPYGFFLETEETFQPGRWFMRAAQAVTAVVCGPDMIQVSLNAGARGDVQQIFQGRNDPMMIYLVWRRIENFAMAQGNSQQTQAGVTVSVGGVDMRAGRIIAWDGQAALHVHNPTQQNAMVQIQVVFYISMDKTLNQYPALTAEIFNVYSFRDHTWHGLRTAILNRTTLPNMLPPIFPPNDRDSILTLLLLSTLADVYTVLRPEFAIHGVNPMPGPLTRAIARAAYV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-05" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q98829</accession>
    <accession>P23066</accession>
    <accession>Q6LCJ9</accession>
    <name>VP6A_BTV10</name>
    <protein>
        <recommendedName>
            <fullName>Protein VP6-A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Minor inner core protein VP6-A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">Segment-9</name>
    </gene>
    <organism>
        <name type="scientific">Bluetongue virus 10 (isolate USA)</name>
        <name type="common">BTV 10</name>
        <dbReference type="NCBI Taxonomy" id="10900"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Duplornaviricota</taxon>
            <taxon>Resentoviricetes</taxon>
            <taxon>Reovirales</taxon>
            <taxon>Reoviridae</taxon>
            <taxon>Sedoreovirinae</taxon>
            <taxon>Orbivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Antilocapra americana</name>
        <name type="common">Pronghorn</name>
        <dbReference type="NCBI Taxonomy" id="9891"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Bos taurus</name>
        <name type="common">Bovine</name>
        <dbReference type="NCBI Taxonomy" id="9913"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Capra hircus</name>
        <name type="common">Goat</name>
        <dbReference type="NCBI Taxonomy" id="9925"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Ovis aries</name>
        <name type="common">Sheep</name>
        <dbReference type="NCBI Taxonomy" id="9940"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Culicoides variipennis</name>
        <name type="common">Biting midge</name>
        <dbReference type="NCBI Taxonomy" id="46212"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1989" name="J. Gen. Virol." volume="70" first="1677" last="1689">
            <title>Completion of the sequence of bluetongue virus serotype 10 by the characterization of a structural protein, VP6, and a non-structural protein, NS2.</title>
            <authorList>
                <person name="Fukusho A."/>
                <person name="Yu Y."/>
                <person name="Yamaguchi S."/>
                <person name="Roy P."/>
            </authorList>
            <dbReference type="PubMed" id="2544660"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2010-01" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Blachere C."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1996" name="J. Virol." volume="70" first="5735" last="5739">
            <title>Phylogenetic comparison of the S3 gene of United States prototype strains of bluetongue virus with that of field isolates from California.</title>
            <authorList>
                <person name="de Mattos C.C."/>
                <person name="de Mattos C.A."/>
                <person name="MacLachlan N.J."/>
                <person name="Giavedoni L.D."/>
                <person name="Yilma T."/>
                <person name="Osburn B.I."/>
            </authorList>
            <dbReference type="PubMed" id="8764098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
        <source>
            <strain>Isolate 10O80Z</strain>
            <strain>isolate BTV10</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Surrounds and interacts with the genomic dsRNA. Possesses ss- and dsRNA-binding capacity. Its hydrophilic nature and capability to bind ss- and dsRNA suggest that it interacts with BTV genomic RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <text>Inner capsid.</text>
    </comment>
    <comment type="alternative products">
        <event type="alternative initiation"/>
        <isoform>
            <id>Q98829-1</id>
            <name>Protein VP6-A</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>P33423-1</id>
            <name>Protein VP6-B</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the reoviruses VP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="D00509">
        <property type="protein sequence ID" value="BAA00399.2"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="EMBL" id="U55781">
        <property type="protein sequence ID" value="AAC55309.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="EMBL" id="U55801">
        <property type="protein sequence ID" value="AAC55329.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007662">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001399">
        <property type="entry name" value="Orbi_VP6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01516">
        <property type="entry name" value="Orbi_VP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00902">
        <property type="entry name" value="VP6CAPSID"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0024">Alternative initiation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000372866" description="Protein VP6-A">
        <location>
            <begin position="1"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="329" mass="35530" checksum="71B6EF82DC19DB18" modified="2011-07-27" version="3">MSAAILLAPGDVIKRSSEELKQRQIQINLVDWMESEGGKEDKTEPKEESKAEGSKDGEGTQSESGQKEEGGKETKDADVDRRIHTAVGSGSGTKGSGERANENANRGDGKVGGGGGDADAGVGATGTNGGRWVVLTEEIARAIESKYGTKIDVYRDEVPAQIIEVERSLQKELGISREGVAEQTERLRDLRRKEKNGTHAKAVERGGRKQRKKAHGDAQREGVEEEKTSEEPARIGITIEGVMSQKKLLSMIGGVERKMAPIGARESAVMLVSNSIKDVVRATAYFTAPTGDPHWKEVAREASKKKNILAYTSTGGDVKTEFLHLIDHL</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>