<?xml version="1.0" encoding="UTF-8"?>
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 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
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<entry dataset="Swiss-Prot" created="2009-07-07" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q87008</accession>
    <name>MVP_SCSVF</name>
    <protein>
        <recommendedName>
            <fullName>Putative movement protein</fullName>
            <shortName>MP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">DNA-M</name>
        <name type="synonym">C1</name>
    </gene>
    <organism>
        <name type="scientific">Subterranean clover stunt virus (strain F)</name>
        <name type="common">SCSV</name>
        <dbReference type="NCBI Taxonomy" id="291607"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Monodnaviria</taxon>
            <taxon>Shotokuvirae</taxon>
            <taxon>Cressdnaviricota</taxon>
            <taxon>Arfiviricetes</taxon>
            <taxon>Mulpavirales</taxon>
            <taxon>Nanoviridae</taxon>
            <taxon>Nanovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Trifolium subterraneum</name>
        <name type="common">Subterranean clover</name>
        <dbReference type="NCBI Taxonomy" id="3900"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1995" name="Virology" volume="207" first="354" last="361">
            <title>Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses.</title>
            <authorList>
                <person name="Boevink P."/>
                <person name="Chu P.W."/>
                <person name="Keese P."/>
            </authorList>
            <dbReference type="PubMed" id="7886939"/>
            <dbReference type="DOI" id="10.1006/viro.1995.1094"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May transport viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Host cell membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">The hydrophobic region is responsible for the localization of the protein to the cell periphery.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nanovirus movement protein family.</text>
    </comment>
    <dbReference type="EMBL" id="U16730">
        <property type="protein sequence ID" value="AAA68017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006543">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0916">Viral movement protein</keyword>
    <feature type="chain" id="PRO_0000378540" description="Putative movement protein">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="77"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="112" mass="12699" checksum="A5FA2266F02B7F13" modified="1996-11-01" version="1">MDSGDGYNTYSYEEGAGDAKKEVLYKIGIIMLCIVGIVVLWVLIILCCAVPRYAKSTMDAWLSSSSIMKRKMASRITGTPFEETGPHRERRWAERRTEATNQNNNDNVNRFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q87009</accession>
    <name>REP2_SCSVF</name>
    <protein>
        <recommendedName>
            <fullName>Para-Rep C2</fullName>
            <shortName>Rep2</shortName>
            <ecNumber>2.7.7.-</ecNumber>
            <ecNumber>3.1.21.-</ecNumber>
            <ecNumber>3.6.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Replication-associated protein of non-essential DNA C2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">C2</name>
    </gene>
    <organism>
        <name type="scientific">Subterranean clover stunt virus (strain F)</name>
        <name type="common">SCSV</name>
        <dbReference type="NCBI Taxonomy" id="291607"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Monodnaviria</taxon>
            <taxon>Shotokuvirae</taxon>
            <taxon>Cressdnaviricota</taxon>
            <taxon>Arfiviricetes</taxon>
            <taxon>Mulpavirales</taxon>
            <taxon>Nanoviridae</taxon>
            <taxon>Nanovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Trifolium subterraneum</name>
        <name type="common">Subterranean clover</name>
        <dbReference type="NCBI Taxonomy" id="3900"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1995" name="Virology" volume="207" first="354" last="361">
            <title>Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses.</title>
            <authorList>
                <person name="Boevink P."/>
                <person name="Chu P.W."/>
                <person name="Keese P."/>
            </authorList>
            <dbReference type="PubMed" id="7886939"/>
            <dbReference type="DOI" id="10.1006/viro.1995.1094"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Divalent metal cations, possibly Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Homooligomer (Potential). Rep binds to repeated DNA motifs (iterons).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text>There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nanoviridea/circoviridae replication-associated protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">This protein is encoded by a subviral DNA that is not present in all isolates of the virus.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-"/>
    <dbReference type="EC" id="3.1.21.-"/>
    <dbReference type="EC" id="3.6.1.-"/>
    <dbReference type="EMBL" id="U16731">
        <property type="protein sequence ID" value="AAA68018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q87009"/>
    <dbReference type="Proteomes" id="UP000006543">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016888">
        <property type="term" value="F:endodeoxyribonuclease activity, producing 5'-phosphomonoesters"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018142">
        <property type="term" value="P:protein-DNA covalent cross-linking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000605">
        <property type="entry name" value="Helicase_SF3_ssDNA/RNA_vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003365">
        <property type="entry name" value="Viral_rep_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00910">
        <property type="entry name" value="RNA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02407">
        <property type="entry name" value="Viral_Rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000378523" description="Para-Rep C2">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RCR-1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RCR-2" evidence="1">
        <location>
            <begin position="45"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="2">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RCR-3" evidence="1">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="2">
        <location>
            <begin position="97"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="For DNA cleavage activity" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="172"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="280" mass="32473" checksum="BAE273C64B04D6E3" modified="1996-11-01" version="1">MARRYCFTLNYATEIERETFLSLFSQDELNYFVVGDETATTGQKHLQGFVSFKNKIRLGGLKKKFGNRAHWEIARGSDSQNRDYCCKETLISEIGIPVMKGSNKRKTMEIYEEDPEEMQLKDPDTALRCKAKKLKEEYCSCYDFQKLRPWQIELHAALMAEPDDRSIIWVYGSDGGEGKTSFAKELIRYGWFYTAGGKTQDVLYMYAQDPERNIAFDVPRCSSEMMNYQAMEMLKNRVFASTKYRPVDLCIRKLVHLIVFANVAPDPTRISEDRLVIINC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-06-10" modified="2022-02-23" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q87010</accession>
    <name>CLINK_SCSVF</name>
    <protein>
        <recommendedName>
            <fullName>Cell cycle link protein</fullName>
            <shortName>Clink</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">DNA-C</name>
        <name type="synonym">C3</name>
    </gene>
    <organism>
        <name type="scientific">Subterranean clover stunt virus (strain F)</name>
        <name type="common">SCSV</name>
        <dbReference type="NCBI Taxonomy" id="291607"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Monodnaviria</taxon>
            <taxon>Shotokuvirae</taxon>
            <taxon>Cressdnaviricota</taxon>
            <taxon>Arfiviricetes</taxon>
            <taxon>Mulpavirales</taxon>
            <taxon>Nanoviridae</taxon>
            <taxon>Nanovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Trifolium subterraneum</name>
        <name type="common">Subterranean clover</name>
        <dbReference type="NCBI Taxonomy" id="3900"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1995" name="Virology" volume="207" first="354" last="361">
            <title>Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses.</title>
            <authorList>
                <person name="Boevink P."/>
                <person name="Chu P.W."/>
                <person name="Keese P."/>
            </authorList>
            <dbReference type="PubMed" id="7886939"/>
            <dbReference type="DOI" id="10.1006/viro.1995.1094"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Interacts with and disrupts the function of host retinoblastoma-related proteins RBR, which are key regulators of the cell cycle. Induces transcriptional activation of E2F-regulated S-phase and G2/M-phase-specific genes. Inactivation of the ability of RBR to arrest the cell cycle leads to the stimulation of viral DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host SKP1. Interacts (via LXCXE domain) with host retinoblastoma-related protein 1 (RBR1). Interacts (via LXCXE domain) with retinoblastoma-related proteins (RBR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nanovirus Clink protein family.</text>
    </comment>
    <dbReference type="EMBL" id="U16732">
        <property type="protein sequence ID" value="AAA68019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q87010"/>
    <dbReference type="Proteomes" id="UP000006543">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000338628" description="Cell cycle link protein">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Binding to host SKP1 protein" evidence="2">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="LXCXE motif, interaction with host RBR" evidence="1">
        <location>
            <begin position="111"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="164" mass="19065" checksum="E315AA435A3B8131" modified="1996-11-01" version="1">MALRYFSHLPEELKEKIMNEHLKEIKKKEFLENVIKAACAVFEGLTKKESVEEDDILRFSGFLEGLSAYYAEATKKKCLVRWKKSVAINLKWRVMEEMHYKLYGFADMEDLYCSELGFPNYGEDDVAYHDGAIVNCKQLEVVFDDLGIEFMSIVIDRGSIKIEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2022-02-23" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q87011</accession>
    <name>NSP_SCSVF</name>
    <protein>
        <recommendedName>
            <fullName>Putative nuclear shuttle protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">DNA-N</name>
        <name type="synonym">C4</name>
    </gene>
    <organism>
        <name type="scientific">Subterranean clover stunt virus (strain F)</name>
        <name type="common">SCSV</name>
        <dbReference type="NCBI Taxonomy" id="291607"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Monodnaviria</taxon>
            <taxon>Shotokuvirae</taxon>
            <taxon>Cressdnaviricota</taxon>
            <taxon>Arfiviricetes</taxon>
            <taxon>Mulpavirales</taxon>
            <taxon>Nanoviridae</taxon>
            <taxon>Nanovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Trifolium subterraneum</name>
        <name type="common">Subterranean clover</name>
        <dbReference type="NCBI Taxonomy" id="3900"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1995" name="Virology" volume="207" first="354" last="361">
            <title>Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses.</title>
            <authorList>
                <person name="Boevink P."/>
                <person name="Chu P.W."/>
                <person name="Keese P."/>
            </authorList>
            <dbReference type="PubMed" id="7886939"/>
            <dbReference type="DOI" id="10.1006/viro.1995.1094"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text>Putative nuclear shuttle protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nanoviridae nuclear shuttle protein family.</text>
    </comment>
    <dbReference type="EMBL" id="U16733">
        <property type="protein sequence ID" value="AAA68020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q87011"/>
    <dbReference type="Proteomes" id="UP000006543">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008706">
        <property type="entry name" value="Nanovirus_C8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05629">
        <property type="entry name" value="Nanovirus_C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000378532" description="Putative nuclear shuttle protein">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="153" mass="17661" checksum="31B0BEF185450F4C" modified="1996-11-01" version="1">MADWFHSALKTCTHVCDFSDIKASSQQDFFCCDSMRGKLSEPRKVLLVSCFVSFTGSFYGSNRNVRGQVQLGMQQDDGVVRPIGYIPIGGYLYHDDYGYYQGEKTFNLDIESDYLKPDEDFWKRFTINIVNDKGLDDRCDVKCYVVHTMRIKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-09-13" modified="2022-02-23" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q87012</accession>
    <name>CAPSD_SCSVF</name>
    <protein>
        <recommendedName>
            <fullName>Capsid protein</fullName>
            <shortName>CP</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Coat protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">DNA-S</name>
        <name type="synonym">C5</name>
    </gene>
    <organism>
        <name type="scientific">Subterranean clover stunt virus (strain F)</name>
        <name type="common">SCSV</name>
        <dbReference type="NCBI Taxonomy" id="291607"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Monodnaviria</taxon>
            <taxon>Shotokuvirae</taxon>
            <taxon>Cressdnaviricota</taxon>
            <taxon>Arfiviricetes</taxon>
            <taxon>Mulpavirales</taxon>
            <taxon>Nanoviridae</taxon>
            <taxon>Nanovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Trifolium subterraneum</name>
        <name type="common">Subterranean clover</name>
        <dbReference type="NCBI Taxonomy" id="3900"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1995" name="Virology" volume="207" first="354" last="361">
            <title>Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses.</title>
            <authorList>
                <person name="Boevink P."/>
                <person name="Chu P.W."/>
                <person name="Keese P."/>
            </authorList>
            <dbReference type="PubMed" id="7886939"/>
            <dbReference type="DOI" id="10.1006/viro.1995.1094"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nanoviridae capsid protein family.</text>
    </comment>
    <dbReference type="EMBL" id="U16734">
        <property type="protein sequence ID" value="AAA68021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q87012"/>
    <dbReference type="Proteomes" id="UP000006543">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006753">
        <property type="entry name" value="Nanovirus_coat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04660">
        <property type="entry name" value="Nanovirus_coat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1140">T=1 icosahedral capsid protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000222437" description="Capsid protein">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="169" mass="18670" checksum="C30B75840122981F" modified="1996-11-01" version="1">MVAVRWGRKGLRSQRRKYSRIAYKPPSSKVVSHVESVLNKRDVTGAEVKPFADGSRYSMKKVMLIATLTMAPGELVNYLIVKSNSPIANWSSSFSNPSLMVKESVQDTVTIVGGGKLESSGTAGKDVTKSFRKFVKLGSGISQTQHLYLIIYSSDAMKITLETRMYIDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q87013</accession>
    <name>REP6_SCSVF</name>
    <protein>
        <recommendedName>
            <fullName>Para-Rep C6</fullName>
            <shortName>Rep6</shortName>
            <ecNumber>2.7.7.-</ecNumber>
            <ecNumber>3.1.21.-</ecNumber>
            <ecNumber>3.6.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Replication-associated protein of non-essential DNA C6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">C6</name>
    </gene>
    <organism>
        <name type="scientific">Subterranean clover stunt virus (strain F)</name>
        <name type="common">SCSV</name>
        <dbReference type="NCBI Taxonomy" id="291607"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Monodnaviria</taxon>
            <taxon>Shotokuvirae</taxon>
            <taxon>Cressdnaviricota</taxon>
            <taxon>Arfiviricetes</taxon>
            <taxon>Mulpavirales</taxon>
            <taxon>Nanoviridae</taxon>
            <taxon>Nanovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Trifolium subterraneum</name>
        <name type="common">Subterranean clover</name>
        <dbReference type="NCBI Taxonomy" id="3900"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1995" name="Virology" volume="207" first="354" last="361">
            <title>Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses.</title>
            <authorList>
                <person name="Boevink P."/>
                <person name="Chu P.W."/>
                <person name="Keese P."/>
            </authorList>
            <dbReference type="PubMed" id="7886939"/>
            <dbReference type="DOI" id="10.1006/viro.1995.1094"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Divalent metal cations, possibly Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Homooligomer (Potential). Rep binds to repeated DNA motifs (iterons).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nanoviridea/circoviridae replication-associated protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">This protein is encoded by a subviral DNA that is not present in all isolates of the virus.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-"/>
    <dbReference type="EC" id="3.1.21.-"/>
    <dbReference type="EC" id="3.6.1.-"/>
    <dbReference type="EMBL" id="U16735">
        <property type="protein sequence ID" value="AAA68022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q87013"/>
    <dbReference type="Proteomes" id="UP000006543">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016888">
        <property type="term" value="F:endodeoxyribonuclease activity, producing 5'-phosphomonoesters"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018142">
        <property type="term" value="P:protein-DNA covalent cross-linking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000605">
        <property type="entry name" value="Helicase_SF3_ssDNA/RNA_vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003365">
        <property type="entry name" value="Viral_rep_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00910">
        <property type="entry name" value="RNA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02407">
        <property type="entry name" value="Viral_Rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000378525" description="Para-Rep C6">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RCR-1" evidence="1">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RCR-2" evidence="1">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="2">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RCR-3" evidence="1">
        <location>
            <begin position="82"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="2">
        <location>
            <begin position="99"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="active site" description="For DNA cleavage activity" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="175"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="285" mass="33500" checksum="EDC1315985477504" modified="1996-11-01" version="1">MPTRQSTSWVFTLNFEGEIPILPFNESVQYACWQHERVGHDHLQGFIQFKSRNTTLRQAKYIFNGLNPHLEIARDVEKAQLYAMKEDSRVAGPWEYGLFIKRGSHKRKLMERFEEDGEEMKIADPSLYRRCLSRKMAEEQRCSSEWNYDLRPWQEEVMHLLEEEPDYRTIIWVYGPAGNEGKSTFARHLSLKDGWGYLPGGKTQDMMHLVTAEPKNNWVFDIPRVSSEYVNYGVIEQVKNRVMVNTKYEPCVMRDDNHPVHVIVFANVLPDLGKLSEDRIKLIRC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2020-08-12" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q87014</accession>
    <name>U1_SCSVF</name>
    <protein>
        <recommendedName>
            <fullName>Protein U1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">DNA-U1</name>
        <name type="synonym">C7</name>
    </gene>
    <organism>
        <name type="scientific">Subterranean clover stunt virus (strain F)</name>
        <name type="common">SCSV</name>
        <dbReference type="NCBI Taxonomy" id="291607"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Monodnaviria</taxon>
            <taxon>Shotokuvirae</taxon>
            <taxon>Cressdnaviricota</taxon>
            <taxon>Arfiviricetes</taxon>
            <taxon>Mulpavirales</taxon>
            <taxon>Nanoviridae</taxon>
            <taxon>Nanovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Trifolium subterraneum</name>
        <name type="common">Subterranean clover</name>
        <dbReference type="NCBI Taxonomy" id="3900"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1995" name="Virology" volume="207" first="354" last="361">
            <title>Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses.</title>
            <authorList>
                <person name="Boevink P."/>
                <person name="Chu P.W."/>
                <person name="Keese P."/>
            </authorList>
            <dbReference type="PubMed" id="7886939"/>
            <dbReference type="DOI" id="10.1006/viro.1995.1094"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nanovirus U1 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="U16736">
        <property type="protein sequence ID" value="AAA68023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006543">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000378536" description="Protein U1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="146" mass="16854" checksum="8390F51353F28342" modified="1996-11-01" version="1">MVSFSFPEIYDVSDDVLVSDSRRSVAVEVEEKVQVINVKVLRLIEAVDEDRVGVKVMFRLCYRYRRELKITLLGCKMELWTSLKSSGKYSVQSLLQRKLNGICVSNYCIGIDMFVSNVKELINRCKWITSVQGVNPICCLYHMDEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q9ICP7</accession>
    <name>MREP_SCSVF</name>
    <protein>
        <recommendedName>
            <fullName>Master replication protein</fullName>
            <shortName>M-Rep</shortName>
            <ecNumber>2.7.7.-</ecNumber>
            <ecNumber>3.1.21.-</ecNumber>
            <ecNumber>3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">DNA-R</name>
        <name type="synonym">C8</name>
    </gene>
    <organism>
        <name type="scientific">Subterranean clover stunt virus (strain F)</name>
        <name type="common">SCSV</name>
        <dbReference type="NCBI Taxonomy" id="291607"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Monodnaviria</taxon>
            <taxon>Shotokuvirae</taxon>
            <taxon>Cressdnaviricota</taxon>
            <taxon>Arfiviricetes</taxon>
            <taxon>Mulpavirales</taxon>
            <taxon>Nanoviridae</taxon>
            <taxon>Nanovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Trifolium subterraneum</name>
        <name type="common">Subterranean clover</name>
        <dbReference type="NCBI Taxonomy" id="3900"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2000" name="Virology" volume="274" first="189" last="195">
            <title>The master rep concept in nanovirus replication: identification of missing genome components and potential for natural genetic reassortment.</title>
            <authorList>
                <person name="Timchenko T."/>
                <person name="Katul L."/>
                <person name="Sano Y."/>
                <person name="de Kouchkovsky F."/>
                <person name="Vetten H.J."/>
                <person name="Gronenborn B."/>
            </authorList>
            <dbReference type="PubMed" id="10936099"/>
            <dbReference type="DOI" id="10.1006/viro.2000.0439"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for the replication of all genomic viral ssDNA (trans-replication). The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-A[GT]TATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Divalent metal cations, possibly Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Homooligomer (Potential). Rep binds to repeated DNA motifs (iterons).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nanoviridea/circoviridae replication-associated protein family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-"/>
    <dbReference type="EC" id="3.1.21.-"/>
    <dbReference type="EC" id="3.6.1.-"/>
    <dbReference type="EMBL" id="AJ290434">
        <property type="protein sequence ID" value="CAB96405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q9ICP7"/>
    <dbReference type="Proteomes" id="UP000006543">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016888">
        <property type="term" value="F:endodeoxyribonuclease activity, producing 5'-phosphomonoesters"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018142">
        <property type="term" value="P:protein-DNA covalent cross-linking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000605">
        <property type="entry name" value="Helicase_SF3_ssDNA/RNA_vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003365">
        <property type="entry name" value="Viral_rep_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00910">
        <property type="entry name" value="RNA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02407">
        <property type="entry name" value="Viral_Rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000378521" description="Master replication protein">
        <location>
            <begin position="1"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RCR-1" evidence="1">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RCR-2" evidence="1">
        <location>
            <begin position="41"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="2">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RCR-3" evidence="1">
        <location>
            <begin position="79"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="2">
        <location>
            <begin position="96"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="active site" description="For DNA cleavage activity" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="180"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="286" mass="33281" checksum="3E49185793F09C58" modified="2000-10-01" version="1">MARQVICWCFTLNNPLAPLSLHESMKYLVYQTEAGDNGTIHYQGYVEMKKRTSLVQMKKLLPGAHLEKRRGSQGEARAYAMKEDSRVEGPWEFGEFKEVLEDKLRSVMEDMKSTGKRPVEYIEDCCNTYDKSSATLREFRGELKKKQAIEEWQLQRQPWMDEVERLMETKDCRRIIWVYGPQGGEGKTSYAKHLVKTRDAFYSTGGKTADIAFAWDHQELVLFDFPRSFEEYVNYGVIEQLKNGIVQSGKYQSIVKYCNYVEVIVFANFIPRSGMFSEDRIVIVYA</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>