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<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QJR4</accession>
    <name>A0A481QJR4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase subunit b</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP synthase F(0) sector subunit b</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">ATPase subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">F-type ATPase subunit b</fullName>
            <shortName evidence="13">F-ATPase subunit b</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 16" type="primary">atpF</name>
        <name evidence="16" type="ORF">C3B55_00007</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0). The b'-subunit is a diverged and duplicated form of b found in plants and photosynthetic bacteria.</text>
    </comment>
    <comment type="function">
        <text evidence="11 13">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 14">Belongs to the ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00007"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01398">
        <property type="entry name" value="ATP_synth_b_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005864">
        <property type="entry name" value="ATP_synth_F0_bsu_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="SSF81573"/>
        <property type="match status" value="1"/>
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    <dbReference type="TIGRFAMs" id="TIGR01144">
        <property type="entry name" value="ATP_synt_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 13 14" id="KW-0138">CF(0)</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 13 14" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 13 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="58"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX81382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="156" mass="17227" checksum="7A75D419106C4C84" modified="2019-06-05" version="1">MNINATLIGQSVAFFIFVVFCMKFIWPPATAALRERQKKIADGLDATARAIHDLGLAQEKASYQLREAKEQAAEIIEQAKKRGNQIVKEAIDQARAEADLLKAAANAEIEHELNSVKDVLRTQLGALVVRGAEKILRATIDRNVHAELVNQLADEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKE6</accession>
    <name>A0A481QKE6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Magnesium and cobalt efflux protein CorC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">corC_1</name>
        <name evidence="12" type="ORF">C3B55_00023</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00023"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="11"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="235"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="302"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="446" mass="50388" checksum="F1C82D5BBDE084E5" modified="2019-06-05" version="1">MDPSNNITFATLFTNFGMIQFTLILVLLNGFFVAAEFAIVKLRATQVEAIAHKNGWRGQILRTVHNELDAYLSACQLGITLASLGLGWIGEPVFAHILESLLDTLGAQSPKVIKGISFFAAFFIISYLHIVVGELAPKSWAIRKPELLSLWTAVPLYLFYWAMYPAIYLLNASANTILRIAGQGEPRPHYEHHYSREELKLILHSSRGQDPSDQGMRVLASAVEMGELEVVDWANSRVDLITLDFNAPLKETLALFRRHKFSRYPLYDTDRNEFVGLLHIKDLLLELAALDHIPESFNLAELTRPLERVSRHMLLSKLLEQFQKGGSHFALVEEADGKVIGYLTMEDVLEVLVGDIQDEHRKAERGILAYQPGKLLVRGDTPLFKLERLLGINLDHIEAETLAGLIYDTMKRVPEEEEVLEIESLRIIIKKMKGPKIILAKVLLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKG2</accession>
    <name>A0A481QKG2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Porphobilinogen deaminase</fullName>
            <shortName evidence="8">PBG</shortName>
            <ecNumber evidence="8">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="8">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">hemC</name>
        <name evidence="11" type="ORF">C3B55_00039</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="8">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Monomer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00039"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018160">
        <property type="term" value="P:peptidyl-pyrromethane cofactor linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="SSF54782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobil_deam" evidence="9">
        <location>
            <begin position="6"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobil_deamC" evidence="10">
        <location>
            <begin position="227"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="8">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="312" mass="34004" checksum="7FDE5DA08A3C5E69" modified="2019-06-05" version="1">MSSCAIRVATRKSALALWQAEHVKTRLKQIYPDLIVTILPIVSSGDKLLNSPLSKIGGKGLFVKELETALLENKADIAVHSMKDVPMDFPEDLDIFCICEREDPRDVFVSNIYTSIDALPLGGIVGTSSLRRKAQLLTLRPDLQIRLLRSNVNHRLMKLDAGEYDAIILAAAGLIRLGLEDRITSAIKVEQSLPAGGQGAVGIECRIADSEIHTLLKLINHVDTEVRITAERAMNRRLNGGCQVPIACYAVLEGENLWLRGLVGDPDGGILLTAEMRGLRRDATALGIQVAEKLLDKGADAILQKVYGMAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKJ0</accession>
    <name>A0A481QKJ0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00144"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="53" mass="6028" checksum="C3A4A613B20BD9C0" modified="2019-06-05" version="1">MLIPCVLKLRRTKIIVQVTNLIAVAIKFIVIYVNSCPFENVLSDMRLQILVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKQ6</accession>
    <name>A0A481QKQ6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">C3B55_00209</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00209"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007016">
        <property type="entry name" value="O-antigen_ligase-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04932">
        <property type="entry name" value="Wzy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="308"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="404" mass="45507" checksum="050D27DBA19D7A02" modified="2019-06-05" version="1">MQSQCLTSGVNRVFDFLVLWILPIGLLLLLSSLFYVENRNVMHRLYYSFFSMPTLLLLCLRPKEIQELLREPLIIAFLAFCIWAIMSVLWSPEPNRDSKFLKAPLQTFMLFAGCGVMLHYRNELFKPILFSAAIIALVVCIDSLIAFAKGFKPGLSMRMIGGLGALDNPLLSSHVFGFFCVYWLCICISTKNLQVFLFSMPALAIMTLAILATGSRMALAALTLSALWIAFVGRNYRAIITITTYMLGTISFLLFNPKIIAERGSSYRFELWGMTLQRIIDQPFIGHGYNSKLSLIINNTELMEPHSFILSVLYYVGIIGLIPWIFMLGWGLHKGLKEQTHPLFLLTSALLIYGIGAGLTEGGDILSRPKEHWFLLWIPLAMIAGLSIAKRRSSMLHMPAPSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKV1</accession>
    <name>A0A481QKV1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Biotin carboxylase</fullName>
            <ecNumber evidence="5 13">6.3.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">Acetyl-coenzyme A carboxylase biotin carboxylase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">accC</name>
        <name evidence="16" type="ORF">C3B55_00250</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + hydrogencarbonate + N(6)-biotinyl-L-lysyl-[protein] = ADP + H(+) + N(6)-carboxybiotinyl-L-lysyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="5 13"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00250"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004549">
        <property type="entry name" value="Acetyl_CoA_COase_biotin_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00514">
        <property type="entry name" value="accC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0092">Biotin</keyword>
    <keyword evidence="13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7 13 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="15">
        <location>
            <begin position="7"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="14">
        <location>
            <begin position="126"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX81611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="452" mass="49215" checksum="A4B4CFE8F33FEF3A" modified="2019-06-05" version="1">MSKPTKKMQKILIANRGEIALRILRACKEEGIKTVAVYSTADTDLMHVKLADESICIGPPLATNSYLKASNIIAAAEVTGADGIHPGYGFLAENADFAEQVEKSGFTFIGPKAETIRLMGDKVSAKAAMIAAGVPTVPGSNGPLPKDNAAAMHIGRTVGYPVIIKATGGGGGHGMRVIHKEEDLIEAAKQICSEATAWFGNPIIYLEKYLTNPRHVEVQVLSDGQGHAVHLGDRDCSLQRRHQKVLEEAPALGLAEKARQEVLARCVKTCIDINYRGAGTFEFLYENGCFFFIEMNTRVQVEHPVSEMVTGIDIIHEMLSIAAGNALSFTQDDVKIHGHSMECRINAEDPKTFTPSPGLVKHFHAPGGNGVRIDSHLYSGYKIPSNYDSLIGKLITWGATRDNAMARMRNALDEIVVDGIKTNIQLHRDLVRDIGFCRGGVNIHYLEHKLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKV2</accession>
    <name>A0A481QKV2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber evidence="9">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">ImGP synthase subunit HisF</fullName>
            <shortName evidence="9">IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">hisF</name>
        <name evidence="11" type="ORF">C3B55_00162</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9 10">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00162"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 10" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7 9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="9">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="256" mass="27454" checksum="51099C9215529075" modified="2019-06-05" version="1">MTLAKRIIPCLDVDNGRVVKGVKFDSIRDAGDPVEIACRYNAQGADEITFLDITASVDKRDTMLYVVERMASQVFIPLTVGGGVRNVQDIRNLLNAGADKVSINTAAVFNPEFVSEAAQHFGSQCIVIAIDAKKVSSLSKTPRWEIFTHGGRKPTNLDAVEWAMKMESLGAGEILLTSIDNDGTKNGFDLSISRIISDTLSIPVIASGGVGNLQHLVDGVIEGHASAVLAASIFHFGEYTVLEAKTYMAACGIVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKV4</accession>
    <name>A0A481QKV4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">General stress protein 69</fullName>
            <ecNumber evidence="2">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">yhdN</name>
        <name evidence="2" type="ORF">C3B55_00213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00213"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="22"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="270" mass="29731" checksum="63A3414746217265" modified="2019-06-05" version="1">MSLSTLHDLHHTLGSTGILVSPLGLGTVKLGRNECVKYPGSFKIPNDDEVRVLLRQAKQLGINLIDTAPAYGRSEKRLGTLLRGQRKEWVLISKVGEEFNDGVPYHDFSATHTRMSIERSLKRLETDFIDLVLVHSDGNDLYILNDCEVYETLAQIKKEGKIRSFGFSSKTVEGGVKALKQGDCAMVTFNLNEQAEKAVIDYAEANSKGILVKKALASGHVCLEPGINLIHKSFILLFAQSVITSAIIGTINPLHLTYNVVTAANIMRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKW1</accession>
    <name>A0A481QKW1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 9">Uroporphyrinogen decarboxylase</fullName>
            <shortName evidence="8">UPD</shortName>
            <shortName evidence="8">URO-D</shortName>
            <ecNumber evidence="4 8 9">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 13" type="primary">hemE</name>
        <name evidence="13" type="ORF">C3B55_00190</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>4 H(+) + uroporphyrinogen III = 4 CO2 + coproporphyrinogen III</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8 9">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 10">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="4 8 9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00190"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 8 9" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen_deCOase" evidence="11">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Uroporphyrinogen_deCOase" evidence="12">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="27"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="354" mass="39093" checksum="4558D86B2690CA60" modified="2019-06-05" version="1">MINLKNDRFLRALFKQPVDVTPVWMMRQAGRYLPEYRASRTNAGDFMSLCMNPEFACEVTMQPLDRYPQLDAAILFSDILTIPDAMGQGLYFETGEGPRFKKVISTIADIEALPIPDPHKDLGYVMSAVSTICRELNGRVPLIGFSGSPWTLATYMVEGSSSKDFRKTKAMLYDNPQAMHLLLDKLAQSVTSYLNGQIIAGVHAVQIFDTWGGNLSAAAYQEFSLAYMRNIISGLVREHEGRKVPVIMFTKGSLWLEGIANAGADALGLDWTYDLGEARRRVGSQVALQGNMDPSVLYAKPEAIRSEVGRILASYGKGSGHVFNLGHGITPEVNPEHAGAFLRAVHELSAQYHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKZ5</accession>
    <name>A0A481QKZ5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA pyrophosphohydrolase</fullName>
            <ecNumber evidence="3">3.6.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">(Di)nucleoside polyphosphate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">rppH</name>
        <name evidence="3" type="synonym">nudH</name>
        <name evidence="6" type="ORF">C3B55_00066</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. RppH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00066"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03671">
        <property type="entry name" value="Ap4A_hydrolase_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00298">
        <property type="entry name" value="Nudix_RppH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022927">
        <property type="entry name" value="RppH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="5">
        <location>
            <begin position="26"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nudix box" evidence="3">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="179" mass="21353" checksum="6A214FF395BDB783" modified="2019-06-05" version="1">MPLNEPLWNNIIISAYEVVAVIDADGFRFNVGIILTNDIGQVLWARRINRVVWQFPQGGINPNETYEDALYRELNEEVGLECKDVRILACTRGWLRYRLPQRLVRTHSQPLCIGQKQKWFLLHLLSNERRVRVDLTNKPEFDSWRWVSYWYPLSQVVTFKREVYRRALKELAPRLLVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL00</accession>
    <name>A0A481QL00_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mig-14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00205"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009977">
        <property type="entry name" value="Mig-14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07395">
        <property type="entry name" value="Mig-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029703">
        <property type="entry name" value="Mig-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="298" mass="33497" checksum="C68B33B2FB19C20F" modified="2019-06-05" version="1">MFNRFQAFRERGWSNVDALTYSDAWHRFGGSVATHPKVVERLAGLAGIPVRYQAWEQNRELKACIATWGPDLALSKSTLQRNGKKGLFDLGNAELILPAAADAQVTLRHRARYLSALNAGRFVGIKLQAEQLAMARTPEELSKKFCYNKRRELRLMEEAGGVVRPVQDFSSADLAAMYCDLFQRRWGFPATGAKHMAQVLKLLRELLIGSVIFLNNAPIAIQLVYRVEAPEWVSVEYINGGVDPKARVFSPGSVLSFLNTQNAWKKAREVGKPLRFSFGRADCAYKNRWCNSVLVFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL10</accession>
    <name>A0A481QL10_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HflC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hflC</name>
        <name evidence="5" type="ORF">C3B55_00231</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">HflC and HflK could regulate a protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the band 7/mec-2 family. HflC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00231"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052547">
        <property type="term" value="P:regulation of peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03405">
        <property type="entry name" value="SPFH_HflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010200">
        <property type="entry name" value="HflC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42911">
        <property type="entry name" value="PTHR42911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005651">
        <property type="entry name" value="HflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01932">
        <property type="entry name" value="hflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="4">
        <location>
            <begin position="21"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="288" mass="32966" checksum="E806648FB9559BED" modified="2019-06-05" version="1">MSNKSLTAMIVGVGVVIIAWNCFYIVSQTERSVLLQFGRVVQADVQPGLHVKIPYVNQVLKFEGRLMTLDAPTQRFLTLEKKAVMVDAYSKWRVKDVERFYTATSGLKQIADERLSRRLESGLRDQFGKRTLHEVVSGERDVLMADITRSLNAMAEKELGIEVADVRVKAIDLPKEVNRSVFERMSTEREREAREHRAKGNELAEGIRADADRQRRVLLAEAYRESEEARGDGDAEAAAIYAKAYSQNQEFYAFYRSLRAYRESFANKTDVMVLDPSSDFFRYLEKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL22</accession>
    <name>A0A481QL22_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="4 6">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 9" type="primary">nadE</name>
        <name evidence="9" type="ORF">C3B55_00242</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="4 6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00242"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_synthase" evidence="8">
        <location>
            <begin position="28"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="50"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="267"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="275" mass="30460" checksum="99ACDD83B3C5CC7C" modified="2019-06-05" version="1">MQSIQREIAQQLKVQAPFKDWTAIDAEVNRRITFIQDCLRNSGLKSLVLGISGGVDSLTAGLLAQKAIKELRTNTGDGSYRFIAVRLPYETQFDEYDANASVDFIKPDEHHTINIGPVVKFMANEVKALQGKTAVSRDFVLGNTKARIRMVTQYTIASAYDGMVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLVKNQVRTIARYFNAPEPLVEKIPTADLENLSPGKPDEVSYGVTYAEIDAFLYGEPVCEKAFKIICDTYYKTRHKRVMPFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL32</accession>
    <name>A0A481QL32_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">23S rRNA (guanosine-2'-O-)-methyltransferase RlmB</fullName>
            <ecNumber evidence="6">2.1.1.185</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">23S rRNA (guanosine2251 2'-O)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">23S rRNA Gm2251 2'-O-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">rlmB</name>
        <name evidence="8" type="ORF">C3B55_00236</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically methylates the ribose of guanosine 2251 in 23S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>guanosine(2251) in 23S rRNA + S-adenosyl-L-methionine = 2'-O-methylguanosine(2251) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10239"/>
            <dbReference type="Rhea" id="RHEA-COMP:10241"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="EC" id="2.1.1.185"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. RlmB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.185" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00236"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070039">
        <property type="term" value="F:rRNA (guanosine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01887">
        <property type="entry name" value="23SrRNA_methyltr_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024915">
        <property type="entry name" value="23S_rRNA_MeTrfase_RlmB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46429">
        <property type="entry name" value="PTHR46429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="7">
        <location>
            <begin position="17"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="263" mass="28903" checksum="1C1B81E25FEAC86E" modified="2019-06-05" version="1">MKAAGYANIRRVMNLENIYGVHAIESLLRHHPKRVKKIWLAKSRSEPRIQVLVELATQNKIVIAQVERSDMDMWVKGVHQGVVANVSPSQIFVETMLDELLDRTESVPLLLVLDGVTDTHNLGACLRSAEAAGALAVIVPKDKSATITPVVRKISCGAAEVIPLVVVTNLSRTLEKLKRRGLWVVGTACEAKISIYDQDLTGPTILVLGAEGKGMRRLTYEYCDYLVYLPMVGSVSSLNVSVATGVCLFEVQRQRSAQVKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL74</accession>
    <name>A0A481QL74_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00394"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="48" mass="5242" checksum="263E7B4FAA96BC32" modified="2019-06-05" version="1">MRVFIADHYASIRFASKVKCCSIVLQTACQVSGFCISYPESGVINVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLB6</accession>
    <name>A0A481QLB6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">4-diphosphocytidyl-2-C-methyl-D-erythritol kinase</fullName>
            <shortName evidence="9">CMK</shortName>
            <ecNumber evidence="2 9">2.7.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">ispE</name>
        <name evidence="12" type="ORF">C3B55_00405</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.148"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the GHMP kinase family. IspE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.148" evidence="2 9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00405"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050515">
        <property type="term" value="F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="PTHR43527:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010376">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5 9 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="10">
        <location>
            <begin position="88"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="11">
        <location>
            <begin position="197"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="283" mass="31059" checksum="A613912F8B44BAE0" modified="2019-06-05" version="1">MPAARITLPSPAKINLMLHILGRREDGYHELQTLFQFLDYGDELTFAVRKDGIIRLHTKFKSVTHNSNLIVRAAKKIQEQSGCLLGIDIWVDKILPIGGGMGGGSSNAATTLLGLNYLWKLDWNYDCLATLGLTLGADVPVFVRGHAAFGDGVGEKLTPEYPEELWYVVLIPQVSVSTVEVFSDPLLTRNSPPIKLHHLIKVNNRNDCLPVVARRYPEVHDALNLLGKFTEAKLTGTGSCVVGGFHSKAEADKVLNCIIKTFAGFVAKGSNVSLLHRTLQHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLC0</accession>
    <name>A0A481QLC0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="2 8">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">glmS</name>
        <name evidence="11" type="ORF">C3B55_00014</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2 8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00014"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00164">
        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GlmS_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="PTHR10937:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8 11" id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="286"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="459"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="8">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="8">
        <location>
            <position position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="610" mass="66688" checksum="B285CCD4A496BA52" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLD0</accession>
    <name>A0A481QLD0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">4-hydroxybenzoate octaprenyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">4-HB polyprenyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">ubiA</name>
        <name evidence="8" type="ORF">C3B55_00024</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of ubiquinone-8 (UQ-8) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate 3-octaprenyl-4-hydroxybenzoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>4-hydroxybenzoate + all-trans-octaprenyl diphosphate = 4-hydroxy-3-all-trans-octaprenylbenzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27782"/>
            <dbReference type="ChEBI" id="CHEBI:1617"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57711"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00024"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002083">
        <property type="term" value="F:4-hydroxybenzoate decaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047293">
        <property type="term" value="F:4-hydroxybenzoate nonaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate octaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PTHR11048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="296" mass="33549" checksum="FD92F602FD70B1D5" modified="2019-06-05" version="1">MYQRLFKSLNCPNLRVWNFIQLARMDKPIGMYLLLWPTLWALWIASNGSPSLRNIVIFVLGVALTRAGGCVINDWADCKIDGHVQRTKQRPLVNSKISTKEALVFFTVLMSISFLLVLMTNTATILLSIGGLALAASYPFMKRYTYYPQVVLGAAFSWGIPMAFTAETSYLPAIAWLLYLANLLWTVSYDTYYAMVDHDDDLKVGIKSTAILFGDAARIIILTMQGLSLACLLLAGILFELGGWFYLGLIVAASYFSCEFWYTRDKNRTKYFKAFLHNHWVGLAIFVGIVADYWFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLD1</accession>
    <name>A0A481QLD1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">rpsD</name>
        <name evidence="11" type="ORF">C3B55_00302</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="7">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00302"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_B">
        <property type="entry name" value="Ribosomal_S4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005709">
        <property type="entry name" value="Ribosomal_S4_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="PTHR11831:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01017">
        <property type="entry name" value="rpsD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S4" evidence="10">
        <location>
            <begin position="3"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="S4" evidence="9">
        <location>
            <begin position="96"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="206" mass="23090" checksum="70EDA759032C1348" modified="2019-06-05" version="1">MARYTGPKCKLARREGTDLFLKSGARAIESKCNIEAVPGIHGQRRGRQSDYGIQLREKQKVRRIYGILECQFRGYYKKAASKKGATGENLLQLLECRLDNVVYRMGFGSTRAESRQLVSHKSVSVNSQTVNVPSYLVGANDVVAIREKAKNQLRIVQALDLRAQRGRVEWVAVDSEKKSGVFKNVPARSDLSADINESLIVELYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLD4</accession>
    <name>A0A481QLD4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rluD</name>
        <name evidence="5" type="ORF">C3B55_00425</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00425"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="4">
        <location>
            <begin position="91"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="323" mass="36320" checksum="8CA467489284A56B" modified="2019-06-05" version="1">MSDTIELRAEVPRKLSGQRLDQVAAQLFSEYSRSSLSVWIKNGCLTVSGVVIRARDIVHGGAILELNAKHKAKETWVAQNIKLDIVYEDDDILVINKPAGLVVHPTIQHADGTLLNALLHHVPDIINVRRCGIVHRLDKDTTGLIVVAKTIQAQMQLIMQLQARSVSRIYECIVIGVVVAGGKINAPIGRHGKKRKRMVVIESGKPAISHYRVLERFRAHTHVLVKLETGRTHQIRVHMAHINFPLVGDQVYGGRCRIPSAASQTMVESLKTFQRQALHARFLEFDHPTSGKRMSWESPLSDDLVWLLSLFKKDCEGMYRMSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLE4</accession>
    <name>A0A481QLE4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00217"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="30" mass="3238" checksum="93BF47B29B8ED473" modified="2019-06-05" version="1">MANKSLEVRKPIGSNQAQLIGRLVDGESIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLE7</accession>
    <name>A0A481QLE7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase J</fullName>
            <ecNumber evidence="1">2.1.1.242</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA m2G1516 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (guanine-N(2)-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">rsmJ</name>
        <name evidence="2" type="ORF">C3B55_00496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the guanosine in position 1516 of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(1516) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(2)-methylguanosine(1516) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43220"/>
            <dbReference type="Rhea" id="RHEA-COMP:10412"/>
            <dbReference type="Rhea" id="RHEA-COMP:10413"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74481"/>
            <dbReference type="EC" id="2.1.1.242"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RsmJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.242" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00496"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008990">
        <property type="term" value="F:rRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01523">
        <property type="entry name" value="16SrRNA_methyltr_J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007536">
        <property type="entry name" value="16SrRNA_methylTrfase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36112">
        <property type="entry name" value="PTHR36112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04445">
        <property type="entry name" value="SAM_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="1">
        <location>
            <begin position="120"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="255" mass="28067" checksum="6CE1DFD40260A19E" modified="2019-06-05" version="1">MTSRIRVEALVNDFQARAEHLAAKLGLPLQLHEADFSLQVSKNGLQLQKLGYEKLGPVHVDFVNGGVAHRRLYGGGSGQMIAKAIGVNKILHPRILDGTAGMGKDAFLLASLGCEIILIERQPLIGALLEDGLARGASDSKVAPIIARMRLLKCNSIEVMLNWKSELPQVIYLDPMFPHRKKTALMKKDMRLFRPIVGDDEDAPALLAAALALAIYRVVVKRPRKASCIEGPKRSYALVGKSNRYDVYHKKTLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLF0</accession>
    <name>A0A481QLF0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="5">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="5">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">truB</name>
        <name evidence="9" type="ORF">C3B55_00376</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00376"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02573">
        <property type="entry name" value="PseudoU_synth_EcTruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015240">
        <property type="entry name" value="tRNA_sdUridine_synth_fam1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="PTHR13767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09157">
        <property type="entry name" value="TruB-C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00431">
        <property type="entry name" value="TruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TruB_N" evidence="6">
        <location>
            <begin position="33"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="TruB_C_2" evidence="8">
        <location>
            <begin position="181"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="TruB-C_2" evidence="7">
        <location>
            <begin position="247"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="312" mass="34424" checksum="FB2C2B9D5A862C5B" modified="2019-06-05" version="1">MAQVKRIRRNVSGIILLDKPIGLTSNSALQKVRWLLNAEKAGHTGSLDPMATGVLPLCFGEATKFSQYLLNSDKNYETIMQLGKTTTTADVEGAVLRVRAVTVSHADIKAVLPAFRGQISQIPPMYSALKRDCQPLYKLARTGEVVERKSRLVNIARLELLSSEGDTARLSVHCSKGTYIRTLVEDIGEQLGCGAYVAELRRTQAGPFTLAQTVKLEELEAVHAEGGNESTDRYLMPSDSGLLDWPLLRFSKHSAFYWLNGQPVRVPGAPKFGMVRVYDYNNCLIGIGEVSEDGRIAPRRLIRSKLSNKCDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLH2</accession>
    <name>A0A481QLH2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">30S ribosomal protein S14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">rpsN</name>
        <name evidence="7" type="ORF">C3B55_00290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts proteins S3 and S10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00290"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00537">
        <property type="entry name" value="Ribosomal_S14_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_S14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023036">
        <property type="entry name" value="Ribosomal_S14_bac/plastid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="PTHR19836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="101" mass="11453" checksum="AAEB9082DF7AF4F4" modified="2019-06-05" version="1">MAKMSMKNRELKRRLAVAKYAKKRAALKAIIINLNANPEVRWEAAVALQKQPRDASASRIRNRCRLTGRPHGVYRKFGLGRNKLREAAMRGDVPGLVKASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLH7</accession>
    <name>A0A481QLH7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00412</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00412"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="39" mass="4617" checksum="23A9DF5FEBC58D71" modified="2019-06-05" version="1">MNRIKESATARHTMMFEQSRSLVGTRETCIQRQLFKLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLI3</accession>
    <name>A0A481QLI3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-hydroxy-3-methylbut-2-enyl diphosphate reductase</fullName>
            <shortName evidence="5">HMBPP reductase</shortName>
            <ecNumber evidence="5">1.17.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">ispH</name>
        <name evidence="6" type="ORF">C3B55_00422</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24488"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24825"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IspH family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.4" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00422"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00097"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051745">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13944">
        <property type="entry name" value="lytB_ispH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00191">
        <property type="entry name" value="IspH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003451">
        <property type="entry name" value="LytB/IspH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30426">
        <property type="entry name" value="PTHR30426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02401">
        <property type="entry name" value="LYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00216">
        <property type="entry name" value="ispH_lytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="226"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="312" mass="34507" checksum="577E09D74DDD396D" modified="2019-06-05" version="1">MQIKLANPRGFCAGVDRAIEIVNRALTVFGPPIYVHHEVVHNKFVVEDLHTRGAIFVEELDQVPNNFIVIFSAHGVSRAVRTQAVSRGLTVFDATCPLVTKVHTAVKRYSRDSRECILIGHAGHPEVKGTIGQYDASNGGAIYLVEEEEDVAKLQVKNPDRLAFVTQTTLSIDDTSRVVGALRNRFQAIRGPSKDNICYATQNRQDAVRKLADECDVVLVFGSQNSSNSNRLRELAERKTTPAYLIDSTEDMQYCWLEGAERIGITAGASAPEVLLQVAIQQLQLWGATYIDELAGRKETITFSIPKELLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLK6</accession>
    <name>A0A481QLK6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Outer-membrane lipoprotein carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">lolA</name>
        <name evidence="10" type="ORF">C3B55_00456</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the lipoprotein should stay in the inner membrane).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the LolA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00456"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044874">
        <property type="term" value="P:lipoprotein localization to outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16325">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00240">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004564">
        <property type="entry name" value="OM_lipoprot_carrier_LolA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018323">
        <property type="entry name" value="OM_lipoprot_carrier_LolA_Pbac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35869">
        <property type="entry name" value="PTHR35869"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03548">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="SSF89392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00547">
        <property type="entry name" value="lolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 9" id="KW-0574">Periplasm</keyword>
    <keyword evidence="8 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019874141" description="Outer-membrane lipoprotein carrier protein" evidence="9">
        <location>
            <begin position="22"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="207" mass="22850" checksum="DCC31BA2686E0B67" modified="2019-06-05" version="1" precursor="true">MYLIRMFLLSVLVVTAVSAHADLASVASLSNLLEKSKTITACFSQLTLDASGTSLQQTKGEIVVQRYGLLYWHTEGKAEQTIVYDGQKVMLWDPDLEQATIKKLDSRLNQTPALLLYGGVSKIKDSFDITSKQTSNVIEFTLTPKSKDTLFDSVQLSFNNGVINNMRLIDSVGHFTDILFSNVKVNGAIDASKLNFIIPNNADVIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLK7</accession>
    <name>A0A481QLK7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">APH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00385"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="124"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="518" mass="58110" checksum="F4DA08135105EFD4" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLL7</accession>
    <name>A0A481QLL7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15 16">Protoporphyrinogen IX oxidase</fullName>
            <shortName evidence="15">PPO</shortName>
            <ecNumber evidence="15 16">1.3.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">C3B55_00129</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Catalyzes the oxidation of protoporphyrinogen IX to protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>3 A + protoporphyrinogen IX = 3 AH2 + protoporphyrin IX</text>
            <dbReference type="Rhea" id="RHEA:62000"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 16">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15 16">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cell membrane</location>
            <topology evidence="3 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15 16">Belongs to the HemJ family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="15 16"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00129"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070818">
        <property type="term" value="F:protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02239">
        <property type="entry name" value="HemJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005265">
        <property type="entry name" value="HemJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40255">
        <property type="entry name" value="PTHR40255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03653">
        <property type="entry name" value="UPF0093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004638">
        <property type="entry name" value="UCP004638"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00701">
        <property type="entry name" value="TIGR00701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="13 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX81499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="142" mass="16552" checksum="54F521AA6405EA49" modified="2019-06-05" version="1">MLYLWIKAFHIVSIVCWFAGLFYLPRLFVYHAQSEDAVNKERFSVMEHRLYHSIMGPAMIATLVFGSWLIQLNQDVFQSGAWIHAKLTLLVILLGYHAMCGIQIKRFASDENTHSHIFYRWFNEVPILILLAIVILVVVKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLM1</accession>
    <name>A0A481QLM1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Glutathione reductase</fullName>
            <ecNumber evidence="12">1.8.1.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">gor</name>
        <name evidence="12" type="ORF">C3B55_00478</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.7" evidence="12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00478"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0274">FAD</keyword>
    <keyword evidence="2 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="11">
        <location>
            <begin position="6"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="10">
        <location>
            <begin position="338"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="138"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="173"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="8">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="452" mass="49998" checksum="68CD716F96C305CE" modified="2019-06-05" version="1">MAYDFDLYVIGAGSGGIRAARFAAGFGARVAIAESHYLGGTCVNVGCIPKKLLVYSAYFAEYFELASSFGWSLDEVNFNWATLITNKNREINRLHGIYRNLLTNSGVILHKGHARLMNPHQVELNGKLFTTKHILIATGSWPNIPRIPGCEYVINSNDVFFLKELPKRVLVVGGGYIAVEFAGIFHGLGAQTTLLYRGDLFLRGFDNSVCMHLREELTRRGLDLKFNVDVERINKQANGSLVTTLKDGRILEVDCVLYATGRRPMLDNLGLENTSIKLDQRGFVQVDNMYQTTETSISAIGDVIGRVQLTPVALAEGMAVARRLFRPKQYRPVNYAMIPTVIFSQPNISAVGQTEEEVKNSGHKVHIFESRFRPMNLTLTNCQEKTLMKLVVNADTDKVLGCHMVGPNAGEIIQGLAIALKSGATKQHFDETIGVHPTAAEEFVTMRTPMVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLM2</accession>
    <name>A0A481QLM2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Deoxyribodipyrimidine photo-lyase</fullName>
            <ecNumber evidence="9">4.1.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">phrB</name>
        <name evidence="9" type="ORF">C3B55_00411</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DNA photolyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.3" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00411"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901363">
        <property type="term" value="F:heterocyclic compound binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097159">
        <property type="term" value="F:organic cyclic compound binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051716">
        <property type="term" value="P:cellular response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018394">
        <property type="entry name" value="DNA_photolyase_1_CS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="PTHR11455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="SSF48173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="SSF52425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00394">
        <property type="entry name" value="DNA_PHOTOLYASES_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0157">Chromophore</keyword>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="8">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="477" mass="54591" checksum="6ECCFF0C6ABA1A4D" modified="2019-06-05" version="1">MHLIWLRTDLRLHDNTALAAACQSGKVAALYLITPQQWLIHNDAPCKVDFWLRNLKHLRQTLGELNIPLLIRTATTWHQAPTVLSKLCHELSIESVHINEEYGIHENQRDAAVAKTLKANGVTLHSYLDQIFFQPGTVLTKTGTYFQVFSQFRKACYNLLSNTLPHLVATPKAQTVIKLKSDPIPEQVVGFIPPSKTLQAIWPAGEKEAHERLNAFVDQQIKRYKDERNFPAKTGTSQLSAYLAAGMISPRQCLHAALQTNDGELENNNIGAITWINELLWREFYKHILVGYPRVSRHLAFRRETEAVVWRQAPNDLAAWQEARTGLPIIDAAMRQLLEIGWMHNRLRMIVAMFLTKNLLIDWREGERFFMRHLIDGDLAANNGGWQWSASTGTDAVPYFRIFNPLKQSKKFDSEGMFIKHWLPELTTLNKKEVHNPEEATKLFGVVNYPRSIIDLKQSRKRAIAAFHNLPSYQGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLM9</accession>
    <name>A0A481QLM9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00318"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="49" mass="5444" checksum="8DBDCE12B83F3F2A" modified="2019-06-05" version="1">MVVPDGPISVLRVKLDRLTLYAWFSVISASCLVGIFVGHTNKFQSIFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLS8</accession>
    <name>A0A481QLS8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00431</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00431"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="80" mass="9442" checksum="315382473B4FAEB9" modified="2019-06-05" version="1">MRLVCFWPFADQQTKRSCKMLSCKTYFEMIFIIGNDSDFFYKHLLQMKLLMIAVINKIANTHGLPVYTKHHAQVQAKFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLT7</accession>
    <name>A0A481QLT7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein-export membrane protein SecG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">secG</name>
        <name evidence="11" type="ORF">C3B55_00369</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in protein export. Participates in an early event of protein translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SecG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00369"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004692">
        <property type="entry name" value="SecG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34182">
        <property type="entry name" value="PTHR34182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03840">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01651">
        <property type="entry name" value="SECGEXPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00810">
        <property type="entry name" value="secG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="109" mass="11441" checksum="99BCAFB3ED425AAE" modified="2019-06-05" version="1">MLETVVVIFHMLTSIGIVALVLLQHGNSADTGMSFGISASNSIFRSQGSSTFLSKITAVLAAGLFITSLGLNYFAKKKADALAQIGLPSQIVLDIQKTKPVSDEAQASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLU9</accession>
    <name>A0A481QLU9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell division protein FtsL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">ftsL</name>
        <name evidence="7" type="ORF">C3B55_00583</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Essential cell division protein. May link together the upstream cell division proteins, which are predominantly cytoplasmic, with the downstream cell division proteins, which are predominantly periplasmic.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of a complex composed of FtsB, FtsL and FtsQ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">Localizes to the division septum where it forms a ring structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FtsL family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00583"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00910">
        <property type="entry name" value="FtsL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011922">
        <property type="entry name" value="Cell_div_FtsL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37479">
        <property type="entry name" value="PTHR37479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04999">
        <property type="entry name" value="FtsL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02209">
        <property type="entry name" value="ftsL_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="97" mass="11033" checksum="655B47E7F465BBB3" modified="2019-06-05" version="1">MSKLFSKPLPRGSLFMLLLFISVLVSAIAVSYSAHYNRQLLNTLYGELIVRDKEQAEWSRLILEQSTWTEHSRIEVLATEQLKMHIPGAAEVHIVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLV6</accession>
    <name>A0A481QLV6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 13">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="8">GluTR</shortName>
            <ecNumber evidence="4 8 13">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 17" type="primary">hemA</name>
        <name evidence="17" type="ORF">C3B55_00407</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 13">
            <text>(S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-glutamyl-tRNA(Glu) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8 13">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 13">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="4 8 13"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00407"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="SSF69075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="SSF69742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01035">
        <property type="entry name" value="hemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GlutR_N" evidence="16">
        <location>
            <begin position="16"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate_DH" evidence="15">
        <location>
            <begin position="180"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="GlutR_dimer" evidence="14">
        <location>
            <begin position="329"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 9">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="197"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="8 12">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX81753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="439" mass="48058" checksum="B7C79B47FCDC8543" modified="2019-06-05" version="1">MAYIHHQRYCMAFLALGINHKTASLDVRERVAFMPEQLVGALQQLCRLTDSREAAILSTCNRSELYIEQKQLSADVILRWLADYHHLSLEALHASAYVHENDAAVRHMMRVASGLDSLVLGEPQILGQMKSAYAVACKAGTIGPLLGRLFQATFNSAKQVRTDTAIGKNPVSVAFAAVSLAKQIFSNLQCSQALLIGAGETITLVARYLHNLGMKRIVVANRTMEHANILAEQFGAHVVLLSDIPAELVHSDIVIASTASQLPILGKGAVESALQLRKHKPIFMVDIAVPRDIKPEVGELDDVYLYSIDDLYAVVAENLKDRYGAAQAAEEMVSTGAADFMVRLRELAAIDVLKEYRQHGERLRDKELTKARRLLANGGSAEEVLMQLARSLTNKLLHAPSVQLKKLTAKGRLDALAMAQELFALGDSVSDSSSDKKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLV8</accession>
    <name>A0A481QLV8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 5">2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">UDP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gtaB</name>
        <name evidence="7" type="ORF">C3B55_00477</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00477"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="PTHR43197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01099">
        <property type="entry name" value="galU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="298" mass="33652" checksum="30B115018534C603" modified="2019-06-05" version="1">MSFIQLRYITYMYPFVKTLMIKKCLFPAAGYGTRFLPVTKAIPKEMLSMVNKPLIQYGVEEAFDAGLSEISIVTGRGKHALEDHFDISYELENQIKGTDQEKYLTGIRKLLNKCSFSYIRQTQIKGLGHAILTGRPLIGNELFAVVLTDDLCVNVTGEGVLTQMVNLYQKYRCTIVAIQEVNPQEINKYGIIAGDYISDGLIRVRHMAEKPALEDAPSNLAIIGRYILTPDIFKLIEETKPDKSGEIQITDALLRQAKNGCVIAYKFKGRRFDCGGAEGYIEAINFCYKYFYKANQAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLX2</accession>
    <name>A0A481QLX2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">rpsE</name>
        <name evidence="12" type="ORF">C3B55_00294</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.</text>
    </comment>
    <comment type="function">
        <text evidence="8">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 10">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00294"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_B">
        <property type="entry name" value="Ribosomal_S5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005712">
        <property type="entry name" value="Ribosomal_S5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01021">
        <property type="entry name" value="rpsE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="11">
        <location>
            <begin position="12"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="166" mass="17799" checksum="78368FC86D29CC79" modified="2019-06-05" version="1">MSNNDQKRDEGYTEKLVQVNRVAKTVKGGRIFTFTALTVVGDSKGRVGFGRGKSREVPAAIQKAMEAARRNLIQVDLNGTTLQYAMKSVHGASKVYMQPASEGTGIIAGGAMRAVLEVAGVQNVLAKCFGSTNPVNVVYATFKALKSMRSPGFIAAKRGLTVREIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLX6</accession>
    <name>A0A481QLX6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA gyrase subunit A</fullName>
            <ecNumber evidence="8">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">gyrA</name>
        <name evidence="10" type="ORF">C3B55_00698</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00698"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034335">
        <property type="term" value="F:DNA negative supercoiling activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01897">
        <property type="entry name" value="GyrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005743">
        <property type="entry name" value="GyrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="9">
        <location>
            <begin position="11"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GyrA-box" evidence="8">
        <location>
            <begin position="561"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="8">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="846" mass="93677" checksum="6574AD5A9FE093C1" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLY5</accession>
    <name>A0A481QLY5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00450"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="78" mass="8755" checksum="50D8D6792B656A13" modified="2019-06-05" version="1">MQSVMNYLSLALTPNLKLPRTKTLQTSANVEAAGVIDIHLYKLLPSNGVLCKHVRFTTKTLYTIMNDSVQIQTVKDTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLZ6</accession>
    <name>A0A481QLZ6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00463"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="87" mass="9934" checksum="8C483602719617CF" modified="2019-06-05" version="1">MKFYLNQLEHVDISPASLIYVILTMLLLFIVTITFTRKAKPCVDLSAAMSNLLANSEEIKQLDISISTDRVFSVNDQMPRKQIWLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM03</accession>
    <name>A0A481QM03_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD assembly factor SdhE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cptB</name>
        <name evidence="5" type="ORF">C3B55_00730</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An FAD assembly protein, which accelerates covalent attachment of the cofactor into other proteins. Plays an essential role in the assembly of succinate dehydrogenase (SDH, respiratory complex II), an enzyme complex that is a component of both the tricarboxylic acid cycle and the electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit SdhA of SDH and other flavinylated proteins as well.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SdhE FAD assembly factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00730"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="SSF109910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="84" mass="10384" checksum="AB0137D1FAC17BFA" modified="2019-06-05" version="1">MVKDVDINCLYWRSRRSMLELDVLLVPFITDVYARLDKVDRELYVRLLTYEDYDIFSWFMERATPKDSELQRMVRMILDHAWFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM24</accession>
    <name>A0A481QM24_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00564"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="58" mass="6549" checksum="0AEF272DD62309DD" modified="2019-06-05" version="1">MLTVVVTGGLAGTAMFTVEIEVIQRNLTNSQVFRHYFYHISKVLVKISALKIRLIIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM27</accession>
    <name>A0A481QM27_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00507"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="95" mass="11175" checksum="B30842B667B2749D" modified="2019-06-05" version="1">MRKDKKQVIGDKIHDNQIKLFLNFEPADMTSPSLHKLIKAYRGLRVDDFERFLVFFKENGLDLDGKNKYNQTFVELVKDQRNAAAYIKLIDNARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM37</accession>
    <name>A0A481QM37_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="10">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="10">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">gatB</name>
        <name evidence="12" type="ORF">C3B55_00536</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00536"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatB_Yqey" evidence="11">
        <location>
            <begin position="328"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="481" mass="53889" checksum="EAB81FE05C4AC534" modified="2019-06-05" version="1">MQWEVIIGLEIHAQLATQSKIFSGSATTFGSEPNTQASLVDLGMPGVLPILNQEAVRMAVMFGLAINAKIGQYNVFTRKNYFYPDLPKGYQISQMELPIVGNGYLDIQLEDCTIKRVGITRAHLEEDTGKSLHEAFPSVTGIDLNRAGMPLLEIVSEPDMRSAKEAVAYVTTVHTLVRYLGICNGNMAQGSLRCDCNVSIRPKGQIEFSARCEIKNINSFRFIEKAINSEVRRQIKLIKDGNKVIQQTRLYDISKDKTRAMRNKEEANDYRYFPDPDLLPVILEDSFLEDIRARLPELPQQKLRRFQKQFGLSAYDARVLTSSCQQANYFEEVVSIAGDAKLSANWVMVELGALLNKQGLRIDDAPITAKQLGCMLLRIKDNTLSGKIAKTVFEAIAADEGSADDIIEKRKLNQVTDSSVILVILDDILTVNVEQVKQYRVADKSKRSKIFNFFVGQAMQASKGKVNPQQVNKLLKDKLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM50</accession>
    <name>A0A481QM50_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00809"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="41" mass="4845" checksum="5639DB6C300F0922" modified="2019-06-05" version="1">MADYFFDNSIRLNINYSITVRVFLRTDNLNLHINHESCIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM73</accession>
    <name>A0A481QM73_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00703</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00703"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="66" mass="8006" checksum="F9495229E1154AEA" modified="2019-06-05" version="1">MLNKVNFLIFNVFLLINKFWSFSNNWILFEIAYAVNLFKRYKLVIFYYCLSYKNGLETVHAANKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM87</accession>
    <name>A0A481QM87_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfurtransferase</fullName>
            <ecNumber evidence="3">2.8.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tusE</name>
        <name evidence="5" type="ORF">C3B55_00459</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of a sulfur-relay system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dsrC/tusE family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00459"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042072">
        <property type="entry name" value="DsrC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025526">
        <property type="entry name" value="DsrC-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043163">
        <property type="entry name" value="DsrC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007453">
        <property type="entry name" value="DsrC/TusE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37010">
        <property type="entry name" value="PTHR37010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04358">
        <property type="entry name" value="DsrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006223">
        <property type="entry name" value="DsrC_TusE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69721">
        <property type="entry name" value="SSF69721"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03342">
        <property type="entry name" value="dsrC_tusE_dsvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="4">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006223-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="111" mass="12556" checksum="F07881E280D14133" modified="2019-06-05" version="1">MNSQNIGKLTLKLDKDGYLIDPNDWSNDIVNVLANAENLKLTADHWEIINLVRDFYVEYQLSPAMRPLIKYIALKIGHRKGNSLYLNKLFNGTPAKLAAKLAGLPRPTNCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM97</accession>
    <name>A0A481QM97_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.293</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pglI</name>
        <name evidence="3" type="ORF">C3B55_00851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.293" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00851"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="11"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="317" mass="36780" checksum="442BCB4F918FB079" modified="2019-06-05" version="1">MSNASLHFDVSVLLVTYNHELYIERTLNSLFSQDFYGSIELVVTDDASSDNTLQYIQRYEGTDARFTFKYLSPSANLGITKNYQRGFLACSSRYVAVLEGDDYWSSPYKLTRQTQFLDEHWECDLCSVNYFIYYENNASFVSRVKRGDGYRLLNARDLILDNLVGNFSTCLYRKSALEALPVELFNIQSYDWIINICVARSSLIGFIEAPMSVYRIHLKNAWHQANRTKQLEMQLSLIPTYDQLTNYVFHASFFKLQLDLRRALVLSRNDKWSSKVARAVAWSSANLEQFISPIVIRLFDEIIPPLIKKIILKILKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM98</accession>
    <name>A0A481QM98_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00611</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00611"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="62" mass="7046" checksum="497769F0788D98B1" modified="2019-06-05" version="1">MIKIATRLHLLDLPLISSVDILLYPISNNGVIGLLLKLSMPYKVCIITLVRVVNFNFRSVHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-04-07" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QME6</accession>
    <name>A0A481QME6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen regulatory protein</fullName>
            <ecNumber evidence="2">2.7.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ptsN</name>
        <name evidence="2" type="ORF">C3B55_00525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00525"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00211">
        <property type="entry name" value="PTS_IIA_fru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002178">
        <property type="entry name" value="PTS_EIIA_type-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006320">
        <property type="entry name" value="PTS_Nitro_regul"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00359">
        <property type="entry name" value="PTS_EIIA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="SSF55804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01419">
        <property type="entry name" value="nitro_reg_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51094">
        <property type="entry name" value="PTS_EIIA_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTS EIIA type-2" evidence="1">
        <location>
            <begin position="6"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="154" mass="17207" checksum="2AA73EC1F7F5F443" modified="2019-06-05" version="1">MIRLETILPLSRSLMNVPGSSKKKAIEEIAKLISREVPDLAEQIVYESLIAREKLGSTGFGNGIAIPHCRLQGCEWPVSALLHMDKPIDFDAIDCIPVDLLFVLLVPQAYIDVHLELLKQIASMLDRKEVRDKLRSASSNEALYQVVLDEQNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QME7</accession>
    <name>A0A481QME7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="6">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">sucC</name>
        <name evidence="9" type="ORF">C3B55_00658</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00658"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_synthase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="PTHR11815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="9"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding; shared with subunit alpha" evidence="6">
        <location>
            <begin position="321"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="388" mass="42052" checksum="1249654A2E5918E8" modified="2019-06-05" version="1">MHIHEYQGKQLLAEYGLPVSKGYVVDTTEAAVKACEKLGGNKWVVKVQVHAGGRGKAGGVKVIHSKEDAQAFAQHWLGKRLVTHQTDTTGQPVTKILVESCTDIAKELYLSVVVDRSSRRIVFMASTEGGVDIEKIAHETPEKILKATINPLVGAQLFQGRELAFQLGLEGKQVVQFAENFINLVTLFLDYDLALLEVNPLVINADGDLHCLDAKIDIDSNAMYRQPKLKTFHDPSQDDPREMHAARFELNYIALEGNIGCMVNGAGLAMGTMDIVKLYGGKPANFLDVGGSATKERVTEAFKIILSDCHVTAVLVNIFGGIVRCDMIAEGIINAVKEVGIKIPVVVRLEGNNSKLGADTLKKSGLNIIVATSLINAAQQVIKVTEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QME8</accession>
    <name>A0A481QME8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">30S ribosomal protein S1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpsA</name>
        <name evidence="6" type="ORF">C3B55_00692</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds mRNA; thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00692"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000110">
        <property type="entry name" value="Ribosomal_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002111">
        <property type="entry name" value="RpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00681">
        <property type="entry name" value="RIBOSOMALS1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00717">
        <property type="entry name" value="rpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="107"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="194"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="279"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="366"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="453"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="564" mass="62681" checksum="BD4381BB03ED1565" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMF2</accession>
    <name>A0A481QMF2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="12">3-ketoacyl-CoA thiolase</fullName>
            <ecNumber evidence="12">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fadA</name>
        <name evidence="12" type="ORF">C3B55_00715</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00715"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012805">
        <property type="entry name" value="FadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02445">
        <property type="entry name" value="fadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="10">
        <location>
            <begin position="11"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="11">
        <location>
            <begin position="268"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="8">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="393" mass="41948" checksum="BFF67D1334D035FF" modified="2019-06-05" version="1">MLMSFNPKHAVIIDFGRTPMGRSNGGMHRNTRAEDMSAHLISKLLERNVKVDPNEIEDVIWGCANQTLEQGWNIARIASLMTKIPHTAAGQTINRLCGSSMSALHAAAQAIMTGNGNVFVVGGVEHMGHVSMTHGVDYNPYMSLYVAKASSQMGLTAEILGKIHGITREAQDAFGLRSHQLAYNATMSGKFKNEIIPINGCDENGFLKLFDNDETIWPDATMERMAALKPVFDSKNGTITSGTSSQIADGASCMIVMSAQRAKDLGIQPMAVIRSMAVAGVDPAIMGYGPVPATQQALKRAGLTISDIDFFELNEAFAAQALPVLKDLRVLDKMNEKVNVHGGAIALGHPFGCSGARISGTLLNVLKQNSGNLGVATMCIGLGQGISTVFECI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMF3</accession>
    <name>A0A481QMF3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH-quinone oxidoreductase subunit H</fullName>
            <ecNumber evidence="5">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NADH dehydrogenase I subunit H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NDH-1 subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">nuoH</name>
        <name evidence="7" type="ORF">C3B55_00441</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cell membrane</location>
            <topology evidence="5 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the complex I subunit 1 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00441"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018086">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="PTHR11432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00667">
        <property type="entry name" value="COMPLEX1_ND1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00668">
        <property type="entry name" value="COMPLEX1_ND1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0874">Quinone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="332" mass="37342" checksum="94810450374E337D" modified="2019-06-05" version="1">MTWLTIEVVDVIVSVVKAIVILFTVIIASAMLSFVERRLLGLWQDRYGPNRVGPFGMFQIAADMLKILFKEDWIPPFADKVIFTLAPLVAMSTLLIAFAIIPITPIWGVANLNVGLLLFFAMASLSVYAVLFAGWSSNNKFALLGSLRGAAQTISYEVFMGIALMGVIVQVGSFNMRDIVEYQAQHSWFIIPQFFGFCTFFIAGIAVTHRHPFDLPEAEQELADGYHIEYSGMKWGMFFIGEYIGIILISAMLVTLFCGGWHCPFNILPQLTFFWFCLKTALFIMLFILVRASIPRPRYDQVMGFSWRFCLPLTLINLLVTATIVLLNTPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMF8</accession>
    <name>A0A481QMF8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00695"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="29" mass="3380" checksum="4848E32C4931571F" modified="2019-06-05" version="1">MRKIIGIQISEIFGNLNNLASTWFESQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMG6</accession>
    <name>A0A481QMG6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP-dependent RNA helicase DeaD</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">deaD</name>
        <name evidence="10" type="ORF">C3B55_00679</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00679"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005580">
        <property type="entry name" value="DbpA/CsdA_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03880">
        <property type="entry name" value="DbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="9">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="37"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="219"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="558" mass="61521" checksum="FDC445151A6737BF" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMG8</accession>
    <name>A0A481QMG8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00706"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="67" mass="7946" checksum="728200F050A62432" modified="2019-06-05" version="1">MLGVFIKILCILRMSFPLYYLNRYFYNKLLTYKEQNMRWSYSLAQLFLCIPILLAAFAAKATPEDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMI2</accession>
    <name>A0A481QMI2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00918"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="53" mass="5907" checksum="4158A21082CFCBFF" modified="2019-06-05" version="1">MCAGISLCDVPQAFTTTIITYLTVKAVPTQINYYCALQVLGYFYVTPLSLRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMI5</accession>
    <name>A0A481QMI5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00566"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="30" mass="3485" checksum="3E5137EE17C5CFB6" modified="2019-06-05" version="1">MGVKVLCFMRKQLGLLAYIIIIDNIYVQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMI8</accession>
    <name>A0A481QMI8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Periplasmic serine endoprotease DegP-like</fullName>
            <ecNumber evidence="7">3.4.21.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mucD</name>
        <name evidence="9" type="ORF">C3B55_00727</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Might be efficient in the degradation of transiently denatured and unfolded proteins which accumulate in the periplasm following stress conditions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.</text>
            <dbReference type="EC" id="3.4.21.107"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.107" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00727"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011782">
        <property type="entry name" value="Pept_S1C_Do"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02037">
        <property type="entry name" value="degP_htrA_DO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="7 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 7" id="KW-0346">Stress response</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019614617" description="Periplasmic serine endoprotease DegP-like" evidence="7">
        <location>
            <begin position="28"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="258"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="378"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR611782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="478" mass="50929" checksum="5434B5AFE0EB6C48" modified="2019-06-05" version="1">MSILRLKSYLFIVATVLILGQTVSAQASELPDFAQLVEQASPAVVNISTTQKLPDRKVSIRQIPDLEGLPPMLREFFERGLPQQRPPRSGSGQREAQSLGSGFIISSDGYILTNNHVIVDADEILVRLADRSELKAKLVGTDQRSDVALLKIESQNLPVLRLGKSQDLKAGQWVAAIGSPFGFDHTVTQGIVSAIGRSLPNENYVPFIQTDVPINPGNSGGPLFNLAGEVVGINSQIYTRSGGFMGVSFAIPIDVAMDVSNQLKSGGKVSRGWLGVVIQEVNKDLAESFHLDKPVGALVAQIQDDGPAAKGGLQVGDVILSINSQPIIMSADLPHLVGALKAGNKAKLEVIRDGKRQTVALTVGAIPEEGMALDALGNAKLGAERSSNRLGIAVVELTDEQKKSLELKSGVVIKEVQDGPAALIGLQSGDVITHLNSQAINSTREFIDIAKALPKNRSVSMRVLHQGSASFITFKLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMJ7</accession>
    <name>A0A481QMJ7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00738"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="81" mass="8942" checksum="9B0FDB263A19C024" modified="2019-06-05" version="1">MHNSGIDMAYKFLSPIELLIDTQVSIGSVTRVKQIIKANPNSVSYLVPVASVLCCCARLLCDTSGCYLLNNSLLIMLHDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMK8</accession>
    <name>A0A481QMK8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease D</fullName>
            <shortName evidence="4">RNase D</shortName>
            <ecNumber evidence="4">3.1.13.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rnd</name>
        <name evidence="6" type="ORF">C3B55_00721</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Exonuclease involved in the 3' processing of various precursor tRNAs. Initiates hydrolysis at the 3'-terminus of an RNA molecule and releases 5'-mononucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Exonucleolytic cleavage that removes extra residues from the 3'-terminus of tRNA to produce 5'-mononucleotides.</text>
            <dbReference type="EC" id="3.1.13.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.5" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00721"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033890">
        <property type="term" value="F:ribonuclease D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042780">
        <property type="term" value="P:tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01899">
        <property type="entry name" value="RNase_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006292">
        <property type="entry name" value="RNase_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="SSF47819"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01388">
        <property type="entry name" value="rnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="HRDC" evidence="5">
        <location>
            <begin position="211"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="375" mass="42522" checksum="D6C35FD67420C5B8" modified="2019-06-05" version="1">MAVDIHWICGNYSLGWHCHEWQQLPFVAIDTEFMRINTFYPLAGLIQIGDGISAYLIDPLTINNWQPLAALLENPAVTKVLHACSEDLEVLRRLTGSLPIPLFDTQLAAAYLNLGFSMGYSRLVRAVLNIELSKGETRSNWLQRPLSEMQISYAIEDAAYLAEVYMRLRSRLSDNKYAWVLEDGAELVSSLRREVNPYEVYCDAKLAWKLSRSQLVVLRELCAWREQQARARDLPRNRIIYERSLWLMATYRPDNLVALGKIACIHQCTLHQDGQALLDLIKHAGSVSMDQWPLAVSKPLPVDAAVLIKQLRALGQAEAQRLKIAPELMLRKKILEVLVKSGYPSGPYKLPDMLRGWRRELMGQALLDGLATVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QML4</accession>
    <name>A0A481QML4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00740"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="67" mass="7766" checksum="30087447BB853903" modified="2019-06-05" version="1">MQPNFNSYLTNYFLADILRLIYFAITSKLEQHVDYALTFRFTVYMLSITSTIFVISIVALNSETSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QML5</accession>
    <name>A0A481QML5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rod shape-determining protein MreD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mreD_1</name>
        <name evidence="10" type="ORF">C3B55_00508</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in formation of the rod shape of the cell. May also contribute to regulation of formation of penicillin-binding proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MreD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00508"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007227">
        <property type="entry name" value="Cell_shape_determining_MreD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026034">
        <property type="entry name" value="MreD_proteobac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37484">
        <property type="entry name" value="PTHR37484"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04093">
        <property type="entry name" value="MreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018472">
        <property type="entry name" value="MreD_proteobac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03426">
        <property type="entry name" value="shape_MreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="162" mass="18625" checksum="358D053E8922284F" modified="2019-06-05" version="1">MAYTNARNSYIVWLNFVIGLLLSVSPLPQFMEILRPLWLALLLAFWSLIMPHTVGLITAMCLGLAEDILYGTLLGQNALLLTIAIFLMQSLQQRLRMFFMWQQCLMILVIFGLMQLVQFWLSALTGNRQPTLALMLPALISALLWPCISFSLRGLRRRYKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QML9</accession>
    <name>A0A481QML9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00867</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00867"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="48" mass="5293" checksum="A1E23F826473B603" modified="2019-06-05" version="1">MPIVPIVFQVCHNTNLGNTARRVGAKHLQLLNFLLILAGNHTTNANAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMN6</accession>
    <name>A0A481QMN6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="9">CTP synthetase</shortName>
            <shortName evidence="9">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">pyrG</name>
        <name evidence="12" type="ORF">C3B55_00754</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00754"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP_synth_N" evidence="11">
        <location>
            <begin position="3"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="GATase" evidence="10">
        <location>
            <begin position="300"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="9">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-glutamine binding" evidence="9">
        <location>
            <begin position="379"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="9">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="514"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="516"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="146"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="186"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="545" mass="60241" checksum="184CFC7CAD6F99A5" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMN7</accession>
    <name>A0A481QMN7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fdxA</name>
        <name evidence="9" type="ORF">C3B55_00780</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00780"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022569">
        <property type="entry name" value="Fd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11953">
        <property type="entry name" value="DUF3470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="107" mass="12251" checksum="C7513EB7E47E7B8E" modified="2019-06-05" version="1">MTFVVTDSCIKCKYTDCVEVCPVDCFYEGPNFLVIHPDECIDCALCEPECPVIAIFSEDEVPAEMQEFIQLNVKLAKIWPNITERKDPMPDAAEWDGKKGKIKYLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMQ3</accession>
    <name>A0A481QMQ3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Acyl carrier protein</fullName>
            <shortName evidence="8">ACP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">acpP</name>
        <name evidence="12" type="ORF">C3B55_00877</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by acpS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00877"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="UniPathway" id="UPA00360"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036104">
        <property type="term" value="P:Kdo2-lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1 8 9" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="11">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="8 9">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="77" mass="8458" checksum="9C3903077E72FF98" modified="2019-06-05" version="1">MSTIEERVKKIVAEQLGVKEEVTSSASFVEDLGADSLDTVELVMALEEEFETEIPDEEAEKITTVQAAIDYVTAHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMQ8</accession>
    <name>A0A481QMQ8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipopolysaccharide export system permease protein LptF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">lptF</name>
        <name evidence="14" type="ORF">C3B55_00561</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex LptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the lipopolysaccharide transport and assembly complex. The LptBFG transporter is composed of two ATP-binding proteins (LptB) and two transmembrane proteins (LptF and LptG).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the LptF/LptG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00561"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030922">
        <property type="entry name" value="LptF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005495">
        <property type="entry name" value="LptG/LptF_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33529">
        <property type="entry name" value="PTHR33529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03739">
        <property type="entry name" value="LptF_LptG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04407">
        <property type="entry name" value="LptF_YjgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="371" mass="41357" checksum="E1E65A2A504858CA" modified="2019-06-05" version="1">MIVFRHLFREVLLTLIAVSAVLLIITMSGRFIKFLTQVASGALDPNALFLIMSFRLPSFLQLILPLGLFLSILLAYGQLHLESEMIVLSATGMSQQRLLAITMVPAAGVALVVAWLSMSLAPHGAMQFQLLLNKQNAMTEFHTLEPGRFQALNNGTRVTYTETMTDDRSNLGGVFISQTNFVQNHKDYGISILVANSGRQEVRPDGSRYLILENGFRYDGSPGLANYRTIKYDTYNILLAKPEASEEVTDCDALPMSSLFGTTEPRSVAVLQWRISLPLLVFIVTLMAVPLSRVNPRQGRFFKLLPAILLYMVYLTTLIAARGYLEKGKLSPAIGLWWVHLVFLVIGLKLFYWESICLKIKSCYLPKGVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMQ9</accession>
    <name>A0A481QMQ9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Cysteine synthase</fullName>
            <ecNumber evidence="5 11">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cysM</name>
        <name evidence="13" type="ORF">C3B55_00837</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5 11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00837"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005858">
        <property type="entry name" value="CysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314:SF100">
        <property type="entry name" value="PTHR10314:SF100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01138">
        <property type="entry name" value="cysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 9 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="22"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="9">
        <location>
            <begin position="191"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX82147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="313" mass="34084" checksum="1BFC933752C71835" modified="2019-06-05" version="1">MMFAYSSLDYEYAMILQYPTIADCVGNTPIIRLQRMADKTTNTLLLKLEGNNPAGSVKDRPALSMITRAELRGQIKPGDTLVEATSGNTGIALAMAATIKGYKMVLIMPDNGSAERKAAITAYGAQLILVTREEGMEGARDLAKCMAAEGHAFVLDQFANSDNPEVHYNSTGPEIWHQTHGTITHFVSSMGTTGTIMGNARYLKEQNSAIQIVGMQPMEGAVIPGIRRWSEEYLPKIYNATRVDRIIDIAQREAEDTTRCLAREEGIFCGVSSGGAVAGMLRLSHEVDNAVIVAIICDRGDRYLSTGIFNEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMR9</accession>
    <name>A0A481QMR9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">O-succinylhomoserine sulfhydrylase</fullName>
            <shortName evidence="2">OSH sulfhydrylase</shortName>
            <shortName evidence="2">OSHS sulfhydrylase</shortName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 5" type="primary">metZ</name>
        <name evidence="5" type="ORF">C3B55_00798</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of L-homocysteine from O-succinyl-L-homoserine (OSHS) and hydrogen sulfide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>hydrogen sulfide + O-succinyl-L-homoserine = L-homocysteine + succinate</text>
            <dbReference type="Rhea" id="RHEA:27826"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from O-succinyl-L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the trans-sulfuration enzymes family. MetZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00798"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00449"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071268">
        <property type="term" value="P:homocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02056">
        <property type="entry name" value="MetZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006234">
        <property type="entry name" value="O-succ-hSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01325">
        <property type="entry name" value="O_suc_HS_sulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="2 3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2 3">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="403" mass="43486" checksum="B013F93452735920" modified="2019-06-05" version="1">MSKEWDAGRLDSNLDGVAFNTLAVRAGQHRTSEGEHSDPIFFTSSYVFRTAADAAARFAGEVPGNVYSRYTNPTVRAFEERIAALEGAELAVAMATGMAAILALVMSLCSSGDHVLVSRSVFGSTISLFERYFKRFGIEVDYVPLADLSGWEASIQTNTKLLFVESPSNPLAELVDINALSKLAHTKGAMLAVDNCFCTPALQQPLKLGADIVVHSATKFIDGQGRCMGGVVAGCGEQMREVLGFMRTAGSALSPFNAWIFLKGLETLSLRMNVHCANAIALAEWLEQQAGIKKVYYAGLKSHPQHMLAKQQQRNFGAVLSFEVRGGKDGAWRFIDATRLISITTNLGDSKTTITHPSTTSHGRLAHQEREAAGIGDSLIRISVGLEDIADLQADITRGLTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMS3</accession>
    <name>A0A481QMS3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00857</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00857"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="88" mass="9712" checksum="4B8DB54E06E1901B" modified="2019-06-05" version="1">MPQIQPCTSCDLLLIAIIAHINVAVFYNSSNKVAQDSCLKFYLAFASVARLNIKVYSMPLNVQTILDLEIAIAQLSKIKNIIVQELCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMS7</accession>
    <name>A0A481QMS7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 13">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="13">ASA dehydrogenase</shortName>
            <shortName evidence="13">ASADH</shortName>
            <ecNumber evidence="8 13">1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">asd</name>
        <name evidence="15" type="ORF">C3B55_00803</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-L-aspartate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 13">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="8 13"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00803"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011534">
        <property type="entry name" value="Asp_ADH_gamma-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46278:SF4">
        <property type="entry name" value="PTHR46278:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01745">
        <property type="entry name" value="asd_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12 13" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="13 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="14">
        <location>
            <begin position="3"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="13">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="37"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="165"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX82118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="370" mass="40966" checksum="E58F770F00FE5BCC" modified="2019-06-05" version="1">MKRVGLIGWRGMVGSVLMQRMLDELDFNLIEPVFFTTSNVYGQGPFVGKDINPLKDAFSIEELKILDVILACQGGAYTNEIFPRLRKSGWQGYWIDAASSLRMHDDAVIILDPVNRKIIDRQLDAGIKNYIGGNCTVSLMLMGLGGLFEAGLVEWMSVMTYQAASGAGAQNMRELIKQMGATHAAVANQLMDPASAILDIDRCVADAMRSDAYPTKMFGVPLAGSLIPWIDKELLNGQSREEWKAQAETNKILGRLKSPIPVDGICVRIGAMRCHTQALTIKLNKDVPITDIEWLISQHNPWVKLVPNSRDISIKELSPTMVTGTLNVPVGRLRKLNMGTKFLGAFTVGDQLLWGAAEPLRRMLRILLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMS9</accession>
    <name>A0A481QMS9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA uridine(34) hydroxylase</fullName>
            <ecNumber evidence="1">1.14.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA hydroxylation protein O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trhO</name>
        <name evidence="3" type="ORF">C3B55_00811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes oxygen-dependent 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + O2 + uridine(34) in tRNA = 5-hydroxyuridine(34) in tRNA + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:64224"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:13381"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136877"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrhO family.</text>
    </comment>
    <dbReference type="EC" id="1.14.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00811"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00469">
        <property type="entry name" value="TrhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020936">
        <property type="entry name" value="TrhO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040503">
        <property type="entry name" value="TRHO_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268">
        <property type="entry name" value="PTHR43268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17773">
        <property type="entry name" value="UPF0176_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="127"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="259" mass="29757" checksum="2D75BB49CB11B44E" modified="2019-06-05" version="1">MTQPIVVAALYKFVTLIDYATIRKPLLQVMVENGIKGTLLIAEEGINGTISGNRESINCLIAWLRKDHRMVNIDYKESYCHYQPFYRTKVKLKKEIVTLGVEGIDTNKKVGTYVNPYDWNELISDLEVLLIDTRNYYEVVIGTFKGAINPKITIFREFPDYIKTNFNPATHKKIAIFCTGGIRCEKASSYMLQEGFEEVYQLKGGILNYLKEMPQEETKWHGDCFVFDNRVTLRHDLSEGGISNAQPYAHAGQYKRSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMT9</accession>
    <name>A0A481QMT9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 9">Chorismate synthase</fullName>
            <shortName evidence="8">CS</shortName>
            <ecNumber evidence="4 8 9">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">aroC</name>
        <name evidence="10" type="ORF">C3B55_00816</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="8 9">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8 9">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the chorismate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="4 8 9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00816"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="PTHR21085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="SSF103263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="7 8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="237"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="292"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="363" mass="38959" checksum="4468374C11DEFDAA" modified="2019-06-05" version="1">MSGNTFGKLFTVTITGESHGQALAAIVDGCPPGLELSLEDLQCDLDRRKPGVSRHATQRQEPDKVEIFSGVFDGRTTGCAIGLLIRNTDQKPKDYSAIKDIFRPAHADYTYHHKYGERDYRGGGRSSARETAMRVAAGAIAKKYLAIEGILIRGYMSQLGQIEIPFKTWSSVNNNAFFSPDPDKVPELEAYMDQLRRDQDSVGAKITVVAEGVKPGLGEPIFDRLDAELAHALMSINAVKGLEIGAGFACVAQRGTEHRDEMTPQGFLSNNAGGILGGISSGQPIVAHLALKATSSITTPGRSIDVHGNPVDVITKGRHDPCAGIRATSIAEAMMAIVLMDHLLRNRGQNANVSVSTPVLGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMU9</accession>
    <name>A0A481QMU9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00933"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="40" mass="4613" checksum="E2FA90B759D33EE5" modified="2019-06-05" version="1">MRQVEKHHIINSLVVTNAALSINKISTMLKRMIHRSLCQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMV1</accession>
    <name>A0A481QMV1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA recombination protein RmuC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rmuC</name>
        <name evidence="4" type="ORF">C3B55_00832</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RmuC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00832"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003798">
        <property type="entry name" value="DNA_recombination_RmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30563">
        <property type="entry name" value="PTHR30563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02646">
        <property type="entry name" value="RmuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="65"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="492" mass="55794" checksum="E5CA7F9E0B7EDC69" modified="2019-06-05" version="1">MVFDWTSFALVITGTAVPLLAVCCQMQLKLNTRIVDCKLLEERLTTAQLLQEGLQIQLDTCHDKINKLSQANAAKQADLAAMRREAELLQINCDRARDAANAWNLDCKTKETELRRLDALSAALNAELREQQDSHKQRLADLQGSRDELRAQFAELAGKIFDEREQRFSETSHKSLSHLLDPLKERIQSFEKRVEESYQNESRERFSLAKELERLQQLNLRLSDEATNLTRALKGQKTQGNWGELILEQVLEHAGLEKGREYQTQVSLKGSDGERFQLDVLIMLPGNKQVVVDSKVSLTAYQQYVAANSEAISQAALRQHVLSLRNHVKSLAGKDYKRLEGLHSLDFVLLFVSIEAAFSAALQIEPNLFQEAFDHHIIIVSPTTLLATLLVIDSFWRHERQNKNAREIAERAGWLYDKFVLFIQDLDEVGSRLQQLDKAYSAARNKLTDGRGNLVGRIEQLRLLGARASKNLPANLLEQAMTDSDSIAHLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMV2</accession>
    <name>A0A481QMV2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable septum site-determining protein MinC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">minC</name>
        <name evidence="9" type="ORF">C3B55_00824</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with MinD and FtsZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the MinC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00824"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901891">
        <property type="term" value="P:regulation of cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00267">
        <property type="entry name" value="MinC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013033">
        <property type="entry name" value="MinC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036145">
        <property type="entry name" value="MinC_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007874">
        <property type="entry name" value="MinC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005526">
        <property type="entry name" value="Septum_form_inhib_MinC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34108">
        <property type="entry name" value="PTHR34108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03775">
        <property type="entry name" value="MinC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05209">
        <property type="entry name" value="MinC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63848">
        <property type="entry name" value="SSF63848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01222">
        <property type="entry name" value="minC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0717">Septation</keyword>
    <feature type="domain" description="MinC_N" evidence="8">
        <location>
            <begin position="8"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="MinC_C" evidence="7">
        <location>
            <begin position="142"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="245" mass="26684" checksum="8767651A5AB1C804" modified="2019-06-05" version="1">MSQTKPLNQDSVFLLKGSILAIIILELACNDLDTLDRQLGAKIALAPSFFKNAPLVLALDKYPSCYGPIDLPNLLRICRSHGLRTLAIRASRIEDITAAIAIELPVLPPSNVRERLLDLMESEVKENPEKSPESIVKPTKIITYPVRGGQQIYAQNSDLVVIAPVSSGAELLADGNIHAYGPMRGRALAGINGNIKARIFCQQLTAELVSIAGQYKVSEDLRRDPLWGARAQVSLSGNVLNILRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMX7</accession>
    <name>A0A481QMX7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">dTDP-4-amino-4,6-dideoxy-D-glucose transaminase</fullName>
            <ecNumber evidence="6">2.6.1.33</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">vioA</name>
        <name evidence="6" type="ORF">C3B55_00845</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.33" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00845"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019179">
        <property type="term" value="F:dTDP-4-amino-4,6-dideoxy-D-glucose transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="357" mass="40273" checksum="AD733E759FAC3667" modified="2019-06-05" version="1">MNITVTKPFMPPYADYQRYLAGIWSREWLTNNGPLLNELEIKLKECLSVKNLLFLSNGTAALQIAIRALELHGEIITTPFSYVATTSSIVWEHCTPVMVDICPDTLNIDANLIEQAITEKTTAILATHVFGNPCDVEAIAVITKKYNLKMIYDAAHAFGTQHKGKSLLAYGDVSTCSFHSTKLFHTVEGGAVISPHANISKRMAMMRNFGHSSANDFGDVGINGKNSEFHAAMGLCNIKHMPEILKVRRRLCERYVQNLKGLPVSLQKILPKTEYNYAYFPVMFESEATLLKLVEIFNLNSIYPRRYFYPSLTKLPYIHGQNASVAEDISRRILCLPLYHTLSLEEVDMICRLVKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN07</accession>
    <name>A0A481QN07_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Electron transfer flavoprotein subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">etfA</name>
        <name evidence="7" type="ORF">C3B55_00869</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00869"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="PTHR43153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ETF" evidence="6">
        <location>
            <begin position="3"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="309" mass="32159" checksum="0FE7DF071FC489DC" modified="2019-06-05" version="1">MTILVIVEHNNKVLSPSASNTVAAAAKIGGDIHILVAGYGAGSVAEAATKIAGVSKVLLADNVAYAYQLPENVAPLVAELSSDYSHILSAATSNGKNILPCVAAQLDVDQISEIISVESANTFKRLIYAGTVVATVQSTAAIKVITVRTISFNPVTTTGASAIIETVSIVHNNSTSNFVSEELVMSDRPELTAAKIVISGGRGMQNSDNFKRLYALADKLGAAVGASRAAVDAGFAHNDIQIGQTGKIVAPQLYIAIGISGAIQHLAGMKDSKVIVAINRDETALIFQIADYGLVSDLFEALLELEKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN12</accession>
    <name>A0A481QN12_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome c-type biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ccmH</name>
        <name evidence="6" type="ORF">C3B55_00670</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Possible subunit of a heme lyase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CcmH/CycL/Ccl2/NrfF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00670"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005616">
        <property type="entry name" value="CcmH/CycL/Ccl2/NrfF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038297">
        <property type="entry name" value="CcmH/CycL/NrfF/Ccl2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03918">
        <property type="entry name" value="CcmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019612747" description="Cytochrome c-type biogenesis protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="154" mass="17631" checksum="5F571AA8EDBD3FD7" modified="2019-06-05" version="1">MKRLLVSVVCMISVAHAAIDTYEFASDAARKRFRDLTKELRCPKCQNQDIADSNAPIAADLRKEIFRMLGEGKNNQQIIDFMVDRYGNFVRYKPPLNGKTALLWFGPAGLLLVGVVIMIMIVRYRYVIPIHRPDALSPEEHKRLNQLLDTKIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN38</accession>
    <name>A0A481QN38_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Imelysin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">C3B55_00905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00905"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018976">
        <property type="entry name" value="Imelysin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038352">
        <property type="entry name" value="Imelysin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09375">
        <property type="entry name" value="Peptidase_M75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019831437" evidence="3">
        <location>
            <begin position="20"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M75" evidence="4">
        <location>
            <begin position="66"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="444" mass="48101" checksum="EBD64086B210581C" modified="2019-06-05" version="1">MIRILLATASLLAITIYFAGCDKGKDKAVTLTQTTNATAQITPPIAIQVNDITAKAVIAHYADIAFAVYSDAELAAKKLQTTIDIFLANPNVKTLKMVRNAWITSRVPYLQSEVFRFGNTIIDDWESQVNAWPLDEGLIDYVDKSYEHALGNSSANANIIANTEIQIGEDVVNIKEITPEKLASLNELGGSEANVATGYHVIEFLLWSQDLNGTGPGAGNRPASDYMTGDGASGGNNERRRTYLHAVTELLVSDLKEMVGNWKPNVTDNYRATLEAEPTINGLRKILFGMGSLSMGELAGERMLAGLEAGSPEDEQDCFSDNTHNSHFYNAKGIRNIYLGEYTRTNGTKITGASLSSLVAKADQVADAELKADLAITEEKIQIMIDHANNGEHYDQLIAAGNYTGNKIVRDAITALVKQTNAIEAAASKLGISNLYLDTANHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN41</accession>
    <name>A0A481QN41_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Integration host factor subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ihfB</name>
        <name evidence="6" type="ORF">C3B55_00691</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 5">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00691"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005685">
        <property type="entry name" value="IHF_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00988">
        <property type="entry name" value="hip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="91" mass="10315" checksum="7369A20B857E8FA0" modified="2019-06-05" version="1">MTKSELIKRIVTNQKLLSYKSVELAIQTILEQMSQCLAAGSRIEIRGFGSFSLHYRAPHVGRNPKTGQFVSLGGKFMPHFKPGKRLRDRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN51</accession>
    <name>A0A481QN51_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquinol oxidase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cyoA</name>
        <name evidence="14" type="ORF">C3B55_00978</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00978"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016682">
        <property type="term" value="F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04212">
        <property type="entry name" value="CuRO_UO_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010514">
        <property type="entry name" value="COX_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034227">
        <property type="entry name" value="CuRO_UO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006333">
        <property type="entry name" value="Cyt_o_ubiquinol_oxidase_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="PTHR22888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06481">
        <property type="entry name" value="COX_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000292">
        <property type="entry name" value="Ubi_od_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="SSF81464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01433">
        <property type="entry name" value="CyoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_TM" evidence="13">
        <location>
            <begin position="21"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_CUA" evidence="12">
        <location>
            <begin position="124"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX82277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="296" mass="33063" checksum="0CE7B5EDC1C82EA7" modified="2019-06-05" version="1">MSKNKYQRLLSFLPLLGMLPTLEGCKWTLLDPKGQVGLDEQNLIITATLMMLLVVIPVIIMTFAFAWKYRASNTSAIYAPKWSHSTKIEITVWLVPILIIIALGSITYKSTHALDPYHSLESNVTPVNIEVVALDWKWLFIYPDLGIATINEIQFPEHTPLNFKITSDTVMNSFFIPALGSQIYAMAGMQTNLHLIANKKAEMDGISANYSGAGFAGMKFKAISTSQEDFIAWVAKVKAAPKLLDQDEYDTLAKQSQNNSVALYSKYMPNLFQKIISKYVGMKPGKQIQHEKSSCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN52</accession>
    <name>A0A481QN52_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00701"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="44" mass="5272" checksum="25D90F594D0AEEAE" modified="2019-06-05" version="1">MVQSVEHYLLRLNSIAISNLDNYHYIKDYIDHHCTAYQLVNILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN56</accession>
    <name>A0A481QN56_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00912</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00912"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="45" mass="4788" checksum="3D7778D3729A26CA" modified="2019-06-05" version="1">MMNIAACQISCVANGLGINTKPFVVKIVHLLALSKIVVASYKSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN57</accession>
    <name>A0A481QN57_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Protein translocase subunit SecA</fullName>
            <ecNumber evidence="14">7.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">secA</name>
        <name evidence="18" type="ORF">C3B55_00920</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="14">Distribution is 50-50.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 15">Belongs to the SecA family.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.8" evidence="14"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00920"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18803">
        <property type="entry name" value="SF2_C_secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="PTHR30612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="SSF81767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="SSF81886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00963">
        <property type="entry name" value="secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 14 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-1278">Translocase</keyword>
    <keyword evidence="12 14 15" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 14 15" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SECA_MOTOR_DEAD" evidence="17">
        <location>
            <begin position="10"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="96"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="112"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="519"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="QAX82223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="918" mass="104433" checksum="BDBC34C9BC5405DC" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN65</accession>
    <name>A0A481QN65_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="8">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">gltX</name>
        <name evidence="12" type="ORF">C3B55_00806</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00806"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1c" evidence="10">
        <location>
            <begin position="4"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_2" evidence="11">
        <location>
            <begin position="334"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="251"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="493" mass="56436" checksum="ABFE08FFA1B61414" modified="2019-06-05" version="1">MITVRTRIAPSPTGDPHVGTAYIALFNYCFAKQYGGEFILRIEDTDQLRSNRKSEQQILDALRWLGITWSEGPDVGGPHGPYRQSERSDIYKQYIKQLVSMGHAFPCFCTAEELNQMRAEQYARGETPRYDGRALLLSKEDVAQRLAAGRPHVIRMKVPSEGVCVVPDMLRGDVEIPWNHIDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLSSAAKLILLYEYFGWELPRLCHMPLLRNPDKSKLSKRKNQTSLTFYERIGFMPEAMLNYLGRMGWSMPDAREKFSLQEMVGNFDMSRVSLGGPVFDIEKLSWLNGQWLRDMPVEKFAMRMQQWAFRPEHTMKIASLVQSRVETFSQVVSLAGFFFTDGVSPDVKLFESKNLSSNQVRQLMQLILWKLESLPQWEKNVIISTIKTVVESMKLTFRDAMPLMFAAITGRANSVSVLDSMEILGLDLTCFRLHQALDLLGGVSEKEKKEWKNLMDLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN71</accession>
    <name>A0A481QN71_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage T7 F exclusion suppressor FxsA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00930"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007313">
        <property type="entry name" value="FxsA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35335">
        <property type="entry name" value="PTHR35335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04186">
        <property type="entry name" value="FxsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="149" mass="16356" checksum="0998007751EA2ECB" modified="2019-06-05" version="1">MRNFLLLFLLSQLLELFVFIQVSSTIGFFPAVLLIILGSMLGFLVLRIAGLTTVLRVSKSLSRGKLPAQPMLTGLMAALAGCLLILPGFISDAVGLSILLLFTCKLLVSKTCHHAFGNDTSYRSSPYIHDTFDCKGDVIEGEFEHRYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNA4</accession>
    <name>A0A481QNA4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00839</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00839"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="59" mass="6205" checksum="A0D128430AF65F80" modified="2019-06-05" version="1">MRIKTSNSKAKPAPAVETSESINSQIADFLKSGGKIQQIAKGVSGQTFGLSKQVGLDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNE2</accession>
    <name>A0A481QNE2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome bo(3) ubiquinol oxidase subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome o ubiquinol oxidase subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Oxidase bo(3) subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquinol oxidase polypeptide III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquinol oxidase subunit 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cyoC</name>
        <name evidence="19" type="ORF">C3B55_00976</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooctamer of two A chains, two B chains, two C chains and two D chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00976"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02863">
        <property type="entry name" value="Ubiquinol_oxidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014206">
        <property type="entry name" value="Cyt_c_ubiqinol_oxidase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033946">
        <property type="entry name" value="Ubiquinol_oxase_su3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="PTHR11403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02842">
        <property type="entry name" value="CyoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="18">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="QAX82275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="203" mass="22685" checksum="06F5CED7BBC268AE" modified="2019-06-05" version="1">MSSLVTKFSNTYAGSHHRDHGSTTIFGFWLYLMTDCILFASIFAVYAVLVNNIADGPSGRDIFELPYVLGETAMLLFSSIIYGLAMLAFDRGNKKSVLGWLALTFLLGLGFITMEIKEFYLLISEGYGPHCSGFLSAFFTLLGTHGLHITAGLLWMAVMIYQVNKHGLTNTNNTRLSCLSLFWHFLDVIWICIFTIVYLMGAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNH1</accession>
    <name>A0A481QNH1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="4 7">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ribH2</name>
        <name evidence="7" type="synonym">ribH</name>
        <name evidence="8" type="ORF">C3B55_00893</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00893"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 7 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="83"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="170" mass="19249" checksum="0D3776D4FAE49DA1" modified="2019-06-05" version="1">MQPTTINNTSKNYHSERVAFIQACWHKDIVDQSRKGFLTEMIALGHQESNIDFFEVSGAFEIPLHAKILAKTNSYAGIVAAALIVDGGIYRHEFVAQSVVGGLMQVQLETEVPVFSVSLTPHHFHASSEHHKFFYEHFVYKGQEAARTCTETLQKMRAIQRYHDSRVTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNJ1</accession>
    <name>A0A481QNJ1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00776"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="32" mass="3654" checksum="406C2AF15EF4C3F7" modified="2019-06-05" version="1">MLNSKSIYLSSKIIDRGMLYNLLVKIITLEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNJ9</accession>
    <name>A0A481QNJ9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9 10">Chromosomal replication initiator protein DnaA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 14" type="primary">dnaA</name>
        <name evidence="14" type="ORF">C3B55_01017</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 11">Belongs to the DnaA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01017"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06571">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1750.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00377">
        <property type="entry name" value="DnaA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001957">
        <property type="entry name" value="Chromosome_initiator_DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020591">
        <property type="entry name" value="Chromosome_initiator_DnaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018312">
        <property type="entry name" value="Chromosome_initiator_DnaA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013159">
        <property type="entry name" value="DnaA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024633">
        <property type="entry name" value="DnaA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038454">
        <property type="entry name" value="DnaA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30050:SF2">
        <property type="entry name" value="PTHR30050:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08299">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 9 10" id="KW-0235">DNA replication</keyword>
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            <end position="332"/>
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            <begin position="413"/>
            <end position="482"/>
        </location>
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        <location>
            <begin position="210"/>
            <end position="217"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018893"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="RuleBase" id="RU004227"/>
        </source>
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        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
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            <dbReference type="SMART" id="SM00760"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="QAX82315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="505" mass="56916" checksum="8FA3F5D8569CE0DD" modified="2019-06-05" version="1">MSVKLWQQCVKLLREELPAQQFNTWIRPLQVEIEGDELRIYAPNRFVLDWVNEKYLSRVLELLNECGNGIVPVLSLLIGSKSSAVPHATPSSTLVITASQTQAVPVNNSLVLVAQTITKSSLQENAFIDHEEPSRDGFDPMAGASSQQAPILTEHRAVQVGGALKHTSYLNSTFTFENFVEGKSNQLARAAAWQVADNPQHGYNPLFLYGGVGLGKTHLMHAVGNHLLNKNQNAKVVYLHSERFVADMVKALQLNAINEFKRFYRSLGALLIDDIQFFARKERSQEEFFHTFNALLEGGQQVILTSDRYPKEIEGLEERLKSRFGWGLTVAVEPPELETRVAILMKKADQAKVYVPHDAAFFIAQRIRSNVRELEGALKRVIAHSHFMGCNITIELIRESLKDLLALQDKLVSVDNIQRTVAEYYKIKISDLLSKRRSRSVARPRQVAMALSKELTNHSLPEIGDVFGGRDHTTVLHACRKINELKESNADIREDYKNLLRTLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNL7</accession>
    <name>A0A481QNL7_9PSED</name>
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        <submittedName>
            <fullName evidence="6">Colicin V production protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cvpA</name>
        <name evidence="6" type="ORF">C3B55_00799</name>
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        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
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            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00799"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009403">
        <property type="term" value="P:toxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Colicin-V_CvpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02674">
        <property type="entry name" value="Colicin_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="184" mass="20142" checksum="E2F7045E5404A879" modified="2019-06-05" version="1">MSFTLADWAIVAIVAISGLISLSRGFVKEALSLLTWILAGVVTWMFGGSLSGYLNRYIETSSLRVIVSCVIMFIVTLIVGAMVNHLICKLIRIKGLSGIDRFLGMSFGIARGGLLVVVGVGLLSLGPVQQDLWWQESILVPKFLLVADWSKNLILSLSSRWLAIVTGLPFNLLFKEQFLLVTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNZ9</accession>
    <name>A0A481QNZ9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Membrane protein insertase YidC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Foldase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 13">Membrane integrase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Membrane protein YidC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">yidC</name>
        <name evidence="15" type="ORF">C3B55_01021</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with the Sec translocase complex via SecD. Specifically interacts with transmembrane segments of nascent integral membrane proteins during membrane integration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the OXA1/ALB3/YidC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01021"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01810">
        <property type="entry name" value="YidC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019998">
        <property type="entry name" value="Membr_insert_YidC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028053">
        <property type="entry name" value="Membr_insert_YidC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038210">
        <property type="entry name" value="YidC_periplasmic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038221">
        <property type="entry name" value="YidC_periplasmic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14849">
        <property type="entry name" value="YidC_periplas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00701">
        <property type="entry name" value="60KDINNERMP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01900">
        <property type="entry name" value="YIDCPROTEIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03593">
        <property type="entry name" value="yidC_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="YidC_periplas" evidence="14">
        <location>
            <begin position="83"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX82319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="560" mass="63211" checksum="1C4F8B363B7D68EB" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QPG4</accession>
    <name>A0A481QPG4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00996</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00996"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="89" mass="10424" checksum="77D0D91B7DA76F4D" modified="2019-06-05" version="1">MLSCTAVITNYRFYADQHYIFSLNSSGYIQTNDKINQFVYYSLLNVSIKYYILTSLSNVYCYPCLRHYFMAISKSTPTVLICTAENLYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QJW4</accession>
    <name>A0A481QJW4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">DNA-directed RNA polymerase subunit omega</fullName>
            <shortName evidence="11">RNAP omega subunit</shortName>
            <ecNumber evidence="2 11">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">RNA polymerase omega subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">Transcriptase subunit omega</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">rpoZ</name>
        <name evidence="12" type="ORF">C3B55_00027</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the RNA polymerase subunit omega family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00027"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00366">
        <property type="entry name" value="RNApol_bact_RpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003716">
        <property type="entry name" value="DNA-dir_RNA_pol_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34476">
        <property type="entry name" value="PTHR34476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00690">
        <property type="entry name" value="rpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 11 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="87" mass="9929" checksum="AD2E9E367C3B323B" modified="2019-06-05" version="1">MARITVEDCLKHVDNRFELVMLSAKRARQLATGNKEPMVQQENDKPTVLALREIARGLMNYELIAKAEIVEDEPLFVAFQEKSNEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK37</accession>
    <name>A0A481QK37_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Diaminopimelate epimerase</fullName>
            <shortName evidence="8">DAP epimerase</shortName>
            <ecNumber evidence="4 8">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">dapF</name>
        <name evidence="10" type="ORF">C3B55_00036</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="4 8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00036"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="210"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="220"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="site" description="Important for dimerization" evidence="8">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="276" mass="30226" checksum="843329C58A856DC0" modified="2019-06-05" version="1">MLLRFTKMHGLGNDFMVLDLVNQHAHILPQQVKQWGNRHTGIGFNQLLIVEAPSNPEVDFYYRIFNSDGSEAEQCGNGARCFARFILDKHLTTKRHIRVETKSGLIDLDIHSDGQISVNMGKPRLVPADIPFQATKQATSYALDVDGTFIDISTVSMGNPHALLRVNDINSAPVYELGPKIEHHPRFPARANVGFLQVIDRSSVELRVWERGSGETQACGTCACAAAVAAISHGWMDSPLLVNLPGGRLSVEWAGPGYPVIMTGPASLVYEGQVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK96</accession>
    <name>A0A481QK96_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00067"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="34" mass="4036" checksum="CF9AD4EE0381B0DB" modified="2019-06-05" version="1">MFNKLRKIVQEIKSVQDLNGCIIDYYVVRLESYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKA9</accession>
    <name>A0A481QKA9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00073</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00073"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="69" mass="7944" checksum="717566D4B070DC87" modified="2019-06-05" version="1">MFSKAQDLPGPLDFRYEIKWSISCIPELFFLVFVADDHDVAGFDFIDQNTMYGAILAFKNFSIAFKQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKB9</accession>
    <name>A0A481QKB9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal RNA small subunit methyltransferase G</fullName>
            <ecNumber evidence="2">2.1.1.170</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">16S rRNA 7-methylguanosine methyltransferase</fullName>
            <shortName evidence="2">16S rRNA m7G methyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">rsmG</name>
        <name evidence="3" type="ORF">C3B55_00003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically methylates the N7 position of guanine in position 527 of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>guanosine(527) in 16S rRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(527) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42732"/>
            <dbReference type="Rhea" id="RHEA-COMP:10209"/>
            <dbReference type="Rhea" id="RHEA-COMP:10210"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.170"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.170" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00003"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070043">
        <property type="term" value="F:rRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00074">
        <property type="entry name" value="16SrRNA_methyltr_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003682">
        <property type="entry name" value="rRNA_ssu_MeTfrase_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31760">
        <property type="entry name" value="PTHR31760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02527">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003078">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00138">
        <property type="entry name" value="rsmG_gidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="214" mass="24041" checksum="75AC94E4AF1887A0" modified="2019-06-05" version="1">MSVLLAYQQADELLIGARQLGVNLTVNQHKLLLGYLAILIKWNKVYNLTAVRNPEQMVSRHLLDSLSVMPFIKNGHWLDVGSGAGIPGIPLAILCPCLQVTCLDSNGKKARFMTQVKLQLKLENLQVLHNQVKKITPELSFNGIIARAFSSLVNFINWTRYLGDLDTHWLAMKGVYPNDELLALPSDFYLHSKYVLAVPGFLGQRHLLVLRRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKC4</accession>
    <name>A0A481QKC4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate carboxylase large subunit</fullName>
            <ecNumber evidence="3">6.4.1.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cfiA</name>
        <name evidence="3" type="ORF">C3B55_00019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.7" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00019"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034029">
        <property type="term" value="F:2-oxoglutarate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005776">
        <property type="entry name" value="OadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01108">
        <property type="entry name" value="oadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="1">
        <location>
            <begin position="526"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="603" mass="66443" checksum="0884AF38A09BD6EE" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKD3</accession>
    <name>A0A481QKD3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Bifunctional protein GlmU</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="15">UDP-N-acetylglucosamine pyrophosphorylase</fullName>
                <ecNumber evidence="15">2.7.7.23</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="15">N-acetylglucosamine-1-phosphate uridyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="15">Glucosamine-1-phosphate N-acetyltransferase</fullName>
                <ecNumber evidence="15">2.3.1.157</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="15 17" type="primary">glmU</name>
        <name evidence="17" type="ORF">C3B55_00013</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="15"/>
    <dbReference type="EC" id="2.3.1.157" evidence="15"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00013"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 15" id="KW-0133">Cell shape</keyword>
    <keyword evidence="14 15" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0511">Multifunctional enzyme</keyword>
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    <keyword evidence="8 15" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="6"/>
            <end position="118"/>
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            <begin position="1"/>
            <end position="226"/>
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            <begin position="227"/>
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            <begin position="248"/>
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            <position position="22"/>
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        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
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            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
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            <name>Mg(2+)</name>
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            <name>acetyl-CoA</name>
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            <name>acetyl-CoA</name>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
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        </source>
    </evidence>
    <sequence length="455" mass="49554" checksum="F89A7563F5A3253D" modified="2019-06-05" version="1">MSLEIIILAAGQGTRMCSTLPKVLHQVAGHSMLSHVIRSARQLKPSRIHIVIGYGADVVHKLLAADDLNFVLQEQQLGTGHATAQATPFISADNVLILYGDVPLIEVRTLRRLLKHLVFGQIGLLTVVLDDPTGYGRIIRDIDGKVVAIVEQKDTCESQRMINEINTGIFAVPANKLANWISRLSNNNVQGEYYVTDVIKMAVSDGWLVATEQPYNSMEVYGVNDRKQLARLERHYQLRAGQHLMAQGVTLLDPARFDVRGEVTVGRDVLIDINVILEGRVIIEDNVVIGPNCIIKDSTLRKGVVVKANSYLEGAVMGEDSEAGPFMRLRFGSVLGARAQVGNFVELKNTYLGEGTKVNHLTYLGDAFIGARTNIGAGTITCNFDGAKKHQTVLGEDVFIGANNSLVAPLRVGDGATTAAGSTINQDVDNFQLGVARASQRNINGWKRPVKTKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKD7</accession>
    <name>A0A481QKD7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <shortName evidence="11">AdoMet synthase</shortName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">MAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 17" type="primary">metK</name>
        <name evidence="17" type="ORF">C3B55_00100</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 13">Belongs to the AdoMet synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00100"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="PTHR11964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="SSF55973"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10 11 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-AdoMet_synt_N" evidence="14">
        <location>
            <begin position="6"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="S-AdoMet_synt_M" evidence="15">
        <location>
            <begin position="115"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="S-AdoMet_synt_C" evidence="16">
        <location>
            <begin position="234"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Flexible loop" evidence="11">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="246"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX81471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="420" mass="45240" checksum="58DB9BECA5213B9B" modified="2019-06-05" version="1">MSEYSIFTSESVSEGHPDKIADQISDAVLDAIIADDKFARVACETLVKTGVAIIAGEVTTTAWVDLEQIVRDMITKIGFNSFDVGFDGATCGVMNIIGKQSPDINQGIDRAKPIDQGAGDQGLMFGYASNETDVLMPAPITFSHQLVQRQATARKNGLLPWLRPDAKSQVTCRYEGGKVIGIDAVVLSTQHNPDVSYADLREGVMELIVKRVLPAELLTKDTRFHINPTGQFIIGGPVSDCGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISLNTFGTGKISDNKLIKLVREIFDLRPYAITTMLDLLHPMYQETAAYGHFGRAPKPKTVGDDTFTTFTWEKTDRANDLRTAAGLKTLDGSLSTHLDSTTGNLLTSQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKF3</accession>
    <name>A0A481QKF3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">30S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5 7" type="primary">rpsU</name>
        <name evidence="7" type="ORF">C3B55_00112</name>
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    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
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            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="71" mass="8428" checksum="90EE93AD112DCE22" modified="2019-06-05" version="1">MPAIKVKENEPFDVALRRFKRSCEKAGVLAEVRSREFYEKPTSERKRKAAAAIKRHAKKVQREQRRAIRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKH0</accession>
    <name>A0A481QKH0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00122"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="42" mass="4505" checksum="E03055AD23D9369E" modified="2019-06-05" version="1">MSDAKPKDSLVCTTLGLPDYVELIAPTKTPSKQGLPIAYNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKJ7</accession>
    <name>A0A481QKJ7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase gamma chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP synthase F1 sector gamma subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">F-ATPase gamma subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">atpG</name>
        <name evidence="10" type="ORF">C3B55_00010</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00010"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00815">
        <property type="entry name" value="ATP_synth_gamma_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="PTHR11693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="SSF52943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="286" mass="31781" checksum="E3B42A54853251D2" modified="2019-06-05" version="1">MTGAKEIQSKIASIKSTQKITDAMEKVAVSKMRKAQIRMVASRPYAERICQIIGHLANANPEYRHQFMINCEVDRIGYVVVSSDRGLCGSLNINLFKVLVKDMEVHYKKGIKIDLCVIGSKGAAFFRNFGGNVVAAINHLGEEPSIHALIGSVKVMLDSYLDGRISRLSIASNKFINRMIQQPAIEQLIPLLATPDQKLKHYWDYLYEPDAKELLDGLIMRYIESQIYQAVVENHAAEQAARMIAMKNATDNATDLISDLQLIYNKARQGAITQEISEIIGGAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKJ8</accession>
    <name>A0A481QKJ8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Thymidylate synthase</fullName>
            <shortName evidence="4">TS</shortName>
            <shortName evidence="4">TSase</shortName>
            <ecNumber evidence="1 4">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">thyA</name>
        <name evidence="6" type="ORF">C3B55_00071</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="1 4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00071"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylat_synt" evidence="5">
        <location>
            <begin position="2"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="126"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="179"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="277" mass="31455" checksum="6129947B4B74D5AF" modified="2019-06-05" version="1">MKQYLALLNEVITKGLTKSDRTSTGTKAVFARQYRHNLADGFPLLTTKKLHFKSIANELIWMLSGNTNIKWLNENSVKIWDKWATEDGNLGPVYGKQWTAWPTKDGGKINQIDYIVCTLKNNPNSRRILFHGWNVEYLPDETKSPQENAHGGKQALPPCHVLYQAFVHDSYLSMQVYIRSSDVFLGLPYNTAALALLTHMLAQQCDLIPHEIIVTTGDTHAYSNHIEQIYTQLARTPKKLPELVINRKPASIYDYKFEDFAIVGYDADPSIKANVAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-08-12" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKK3</accession>
    <name>A0A481QKK3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Porin D</fullName>
            <ecNumber evidence="3">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">oprD</name>
        <name evidence="3" type="ORF">C3B55_00153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane porin (Opr) (TC 1.B.25) family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00153"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005318">
        <property type="entry name" value="OM_porin_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34596">
        <property type="entry name" value="PTHR34596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03573">
        <property type="entry name" value="OprD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019862347" evidence="2">
        <location>
            <begin position="23"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="408" mass="44961" checksum="631BE908BD14BB69" modified="2019-06-05" version="1">MLKQRMGLIAIGFFSVSQVAMANGQEQSKGFIEDGDSNIIIRNSYIGRDYKNGKQDKVTWGQSFTSKYESGFTQGTVGVGVDITGQYAIRLNSGTGSSGEAAYDLARGSVAAKARISGTIIKYGQQIPNVPVLQYDNSRLLSEIYEGTSIVSKEIAGLQLDASHLTKKEACRGARGSDSGRAETYLKNIDYIGGSYKFTEGLSAALYVSNMQDMLKKQYINVKYMLALPKKQSLTFNFNGYNTKLDRSFAIEKHGNADARDNKIWSLSATWAIGPHSFTVVHQRSNGDIGSIGDAPLLANSYWSDFNSKDERSWQAGYGLNFSVFGMPELTYSVAYVHGSNIDDGSNRGNGTELEIFNQFRYVIRNGPAKDITLRARASWLRVSSNVSNYNVSGNEIRLFADYPINLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKK6</accession>
    <name>A0A481QKK6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <shortName evidence="10">PCK</shortName>
            <shortName evidence="10">PEP carboxykinase</shortName>
            <shortName evidence="10">PEPCK</shortName>
            <ecNumber evidence="4 10">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">pckA</name>
        <name evidence="11" type="ORF">C3B55_00148</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="4 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00148"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
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        </source>
    </evidence>
    <sequence length="513" mass="56137" checksum="C892DEB9C3E48348" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-10-07" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKL7</accession>
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        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
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            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">HWA_ENA</strain>
        </source>
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        </source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81448.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKQ3</accession>
    <name>A0A481QKQ3_9PSED</name>
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        </recommendedName>
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        <lineage>
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            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
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            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
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            <dbReference type="ChEBI" id="CHEBI:58613"/>
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        </reaction>
    </comment>
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    </comment>
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    </comment>
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    </dbReference>
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        <property type="reaction ID" value="UER00043"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
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        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="IGPS" evidence="9">
        <location>
            <begin position="7"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="277" mass="30591" checksum="3734BAAB63420F1A" modified="2019-06-05" version="1">MSTPTFLEKILSRKVKEVAERRIRVSLAELESMVKIAAAPLGFANAMISRAKLKHPAVIAEIKKASPSKGVIRKNFVPADIAISYEKGGATCLSVLTDIDFFQGSDLFLQQARTACKLPVIRKDFIVDPYQIIEARALGADCVLLIVSILDNVKIAELAAVAKSVGLDVLVEVHNNDELERALKILDTPLVGVNNRNLHTFEVSFDNIFDILSRIPCDRLVIAESGIVNRADVDLMELSGVYAFLVGETFMRAEDPGLELQRLFFPDRSMAFSDFCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKQ8</accession>
    <name>A0A481QKQ8_9PSED</name>
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        <submittedName>
            <fullName evidence="1">Esterase YqiA</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
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            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00218"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008886">
        <property type="entry name" value="UPF0227/Esterase_YqiA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35602">
        <property type="entry name" value="PTHR35602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05728">
        <property type="entry name" value="UPF0227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="202" mass="22460" checksum="F771894A05F760E0" modified="2019-06-05" version="1">MSASILYIHGFNSAPASNKATKLTTVMDSLGLGNQLRVPALHHHPRQAIPQLEDAIMHLGRPLLVGSSLGGYYATHLAERYGLKALLVNPVVSPHLMFKGYLGTQRNLYTDEIWELTHDHVTALAELEVPAPQDAKRYQVWLQTGDKTLDYRLAKQYYCACVLRIQIGGDHGYQGFAQQLPAILSFADINADHYQSFDFAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKS8</accession>
    <name>A0A481QKS8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00140"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="86" mass="10199" checksum="1036B21AF982B54E" modified="2019-06-05" version="1">MIDMPKSSWKYADEKDLTNMPFHSCSKQVIYVFPLILLFVLTLGFYIFIMRNFYLIQYAQLVYLIMVDKNYPSTTLSFCSVRARCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKU3</accession>
    <name>A0A481QKU3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tim44-like domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">C3B55_00015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00015"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019840434" evidence="3">
        <location>
            <begin position="24"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Tim44" evidence="4">
        <location>
            <begin position="150"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="284" mass="31043" checksum="B7C1DF482365717E" modified="2019-06-05" version="1">MKRFFVITMALCLSMMASLDVNAKRFGGGKNIGSTPTHQTSQTTTAASNTSATSATTGTTSASRWLGPLAGIAAGGLLASMLMGGGFHGMQILDILIIMIVAFVIVRLIASRRRKQLEYIAPAGAPRQHEVSQPSLAMNSVLGDSATYTTSATSRAGAYAPLWFNEKRFLEEAYSHFQSLQQHWDANEIEKIAEFVTPQLLELLKSERTEIGNTFQSTYIENLHVQLDGIDDRADKTVATLTFSGTSKNSRFDQGEAFSESWNIERIQGDNQVWLVAGIRQNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKV0</accession>
    <name>A0A481QKV0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis accessory factor UbiJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiJ</name>
        <name evidence="3" type="ORF">C3B55_00178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for ubiquinone (coenzyme Q) biosynthesis. Binds hydrophobic ubiquinone biosynthetic intermediates via its SCP2 domain and is essential for the stability of the Ubi complex. May constitute a docking platform where Ubi enzymes assemble and access their SCP2-bound polyprenyl substrates.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00178"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032150">
        <property type="term" value="P:ubiquinone biosynthetic process from chorismate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02215">
        <property type="entry name" value="UbiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038989">
        <property type="entry name" value="UbiJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38693">
        <property type="entry name" value="PTHR38693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SSF55718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="SCP2" evidence="2">
        <location>
            <begin position="14"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="200" mass="22851" checksum="73CE251DDEE9E713" modified="2019-06-05" version="1">MLFVDCLTMVEYGLNRILSLDSTVWTRLAHLNGKIIAVNCISPTLRLFILLVDKGLLLSSDWTTKADCTMHAPASSWLHLALSHNKTAVLHSSEVKIEGDSSVLLNLVTVLQDLELDWEYELSHWIGPIATQLVNWHLRSCSIWCKQLFTGINQNITEYLSEESRTIVGQREAQARFGELDKAKIKLEYLEARFKRLNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKW0</accession>
    <name>A0A481QKW0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATP-dependent RNA helicase RhlE</fullName>
            <ecNumber evidence="11">3.6.4.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rhlE</name>
        <name evidence="11" type="ORF">C3B55_00096</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00096"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="32"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="223"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="385"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="489"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="387"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="412"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="489"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="569" mass="63844" checksum="FCD81F7B643F2857" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKW2</accession>
    <name>A0A481QKW2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 12" type="primary">rplK</name>
        <name evidence="12" type="ORF">C3B55_00266</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="PTM">
        <text evidence="7 9">One or more lysine residues are methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00266"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_L11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_L11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L11_N" evidence="11">
        <location>
            <begin position="9"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L11" evidence="10">
        <location>
            <begin position="73"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="143" mass="15100" checksum="B9400DBF5700ED42" modified="2019-06-05" version="1">MAKKITAYIKLQVKAAQANPSPPIGPALGQHGLNIMEFCKSFNARTQGLELGLPTPVIITVYSDRSFTFETRSAPASVLLKKAVNVTSGSARPNTVKVGVVTRAQLEEIAKTKNMDLTAADMNAAVRTIAGSARSMGLDVEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKW5</accession>
    <name>A0A481QKW5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Homoserine O-succinyltransferase</fullName>
            <shortName evidence="2">HST</shortName>
            <ecNumber evidence="2">2.3.1.46</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Homoserine transsuccinylase</fullName>
            <shortName evidence="2">HTS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">metX</name>
        <name evidence="2" type="synonym">metXS</name>
        <name evidence="5" type="ORF">C3B55_00084</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-homoserine + succinyl-CoA = CoA + O-succinyl-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:22008"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="EC" id="2.3.1.46"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.46" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00084"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00075"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008899">
        <property type="term" value="F:homoserine O-succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="PTHR32268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="51"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2 3">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="active site" evidence="2 3">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="active site" evidence="2 3">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for acyl-CoA specificity" evidence="2">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000443-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="380" mass="41887" checksum="2562B7F36981D125" modified="2019-06-05" version="1">MPTAFPPDSVGLVAPKVAHFSEPLALACGRSLPNYDLIYETYGQLNAMVSNAVLISHALSGHHHAAGFHSIYERKPGWWDSCIGPGKPIDTNKFFVVSLNNLGGCNGSTGPSSLNPATGKPFGADFPVLTVEDWVHSQARLANLLGIAQWAAVIGGSLGGMQALQWTISYPDRVRHCLAIASAPKLSAQNIAFNEVARQAILTDPEFHGGFFQEAGVIPKRGLMLARMVGHITYLSDNSMGEKFGRGLKNEKLNYDFHSVEFQVESYLRYQSEEFSGRFDANTYLLMTKALDYFDPAASYDNDLAKTFEQTTAKFCVMSFTTDWRFSPARSRELVDALMAARKDVCYLEIDAPQGHDAFLIAIPRYLQAFSNYMNCITLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKY6</accession>
    <name>A0A481QKY6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Bifunctional protein HldE</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="11">D-beta-D-heptose 7-phosphate kinase</fullName>
                <ecNumber evidence="11">2.7.1.167</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">D-beta-D-heptose 7-phosphotransferase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="11">D-glycero-beta-D-manno-heptose-7-phosphate kinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="11">D-beta-D-heptose 1-phosphate adenylyltransferase</fullName>
                <ecNumber evidence="11">2.7.7.70</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">hldE</name>
        <name evidence="14" type="ORF">C3B55_00211</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 11">Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.</text>
    </comment>
    <comment type="function">
        <text evidence="2 11">Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + D-glycero-beta-D-manno-heptose 1-phosphate + H(+) = ADP-D-glycero-beta-D-manno-heptose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59967"/>
            <dbReference type="ChEBI" id="CHEBI:61593"/>
            <dbReference type="EC" id="2.7.7.70"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27473"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60204"/>
            <dbReference type="ChEBI" id="CHEBI:60208"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.167"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Bacterial outer membrane biogenesis; LPS core biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">In the C-terminal section; belongs to the cytidylyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">In the N-terminal section; belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.167" evidence="11"/>
    <dbReference type="EC" id="2.7.7.70" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00211"/>
    <dbReference type="UniPathway" id="UPA00356">
        <property type="reaction ID" value="UER00437"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00958"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033785">
        <property type="term" value="F:heptose 7-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033786">
        <property type="term" value="F:heptose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097171">
        <property type="term" value="P:ADP-L-glycero-beta-D-manno-heptose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009244">
        <property type="term" value="P:lipopolysaccharide core region biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01172">
        <property type="entry name" value="RfaE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01603">
        <property type="entry name" value="HldE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023030">
        <property type="entry name" value="Bifunc_HldE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011913">
        <property type="entry name" value="RfaE_dom_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011914">
        <property type="entry name" value="RfaE_dom_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02198">
        <property type="entry name" value="rfaE_dom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02199">
        <property type="entry name" value="rfaE_dom_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="12">
        <location>
            <begin position="11"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="CTP_transf_like" evidence="13">
        <location>
            <begin position="344"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ribokinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Cytidylyltransferase" evidence="11">
        <location>
            <begin position="343"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="194"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="475" mass="50667" checksum="9E70B73BEE589714" modified="2019-06-05" version="1">MKLSMPRFDQAHVLVVGDVMLDRYWHGDTLRISSESPVPVVNVKHIEDRPGGAANVALNIAALGATASLVGVIGDDEAASSLSNSLRSAGVHTLFQCIPHQPTIVKLRVMSRHHQLMRIDFEESFVTDAMVLGEEIYELFESINVLVLSDYGKGTLKNHQVLIQAAKALNIPVLADPKGKDFSVYRGASLITPNLNEFEAIVGSCADEHELVTKGSVLMNNLDLGALLVTRGEHGMTLLHPDHLAIHLPARAREVFDVTGAGDTVMSTLSASIAAGEKLANSVALANLAAGIVVGKLGTAAISAPGLRRAIQRYEGLERGVLAIEQLLLAIDDARAHNESIVFTNGCFDILHTGHVAYLEQARAQGDRLVVAVNDDASVSRLKGSGRPINSVNRRMAVLAGLGAVDWVVSFSEDTQKSLLAQVKPDVLVKGGDYSVAQVVGADIVSAYGGTVKVLSMVEDSSTTAIVEKIRNYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKZ1</accession>
    <name>A0A481QKZ1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secY</name>
        <name evidence="11" type="ORF">C3B55_00297</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00297"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="442" mass="48597" checksum="8137AA3C84DFE054" modified="2019-06-05" version="1">MAKQSALSALGQGGMPELWVRLRFLFLAIIVYRIGAHIPVPGINPNQLVDLFRHNEGTILSLFNMFSGGALERMSIFAMGIMPYVSASIIMQLMIAASPQLEQLKKEGEAGRCKINLYTRYGAVVLALIQAISISIGLVEHEVTLTHDFGFYFIAVSTFVAGSMFMMWLGEQITERGIGNGISMLIFAGIVAGLPKAIGQSFESARQGDINIIALIIIGFLAVVIIGFVVFIERGQRRIVVHYARRQQGRKVFAAQTSHLPLKVNMAGVIPAIFASSILLFPASLGTWFGQSENMYWLQGLVQLIAPGQPLNVLLFSVGIIFFCFFYTALIFNPKDVAENLKKSGAFIPGIRPGEQSARYINVVLTRLTLFGALYIMVVCLLPQFLVVAANVPFYLGGTSLLIVVVVVMDFMSQVQSHLFSHQYESLIKKANLKSCSSSMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKZ2</accession>
    <name>A0A481QKZ2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00223"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="56" mass="6214" checksum="99B2E2B280B5D9B2" modified="2019-06-05" version="1">MVANIFIHLTMLVLIAYAIIIGVIIDVLSMCTKHHDRLAMSIQLFEMLIIDSASIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKZ6</accession>
    <name>A0A481QKZ6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00138"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="64" mass="7044" checksum="717CC8C8F1113069" modified="2019-06-05" version="1">MNQYCERNKLSAYLCFLLSARSAINNGSGFISILVLVQVKQVINIFTNAAHFIIGVVSARLILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKZ7</accession>
    <name>A0A481QKZ7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylserine decarboxylase proenzyme</fullName>
            <ecNumber evidence="11">4.1.1.65</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="11">Phosphatidylserine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="11">Phosphatidylserine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">psd</name>
        <name evidence="13" type="ORF">C3B55_00221</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:20828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="4.1.1.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="11">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="11">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Prokaryotic type I sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00221"/>
    <dbReference type="UniPathway" id="UPA00544"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00616"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00662">
        <property type="entry name" value="PS_decarb_PSD_B_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033178">
        <property type="entry name" value="PSD_type1_pro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PTHR10067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10 11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 11" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023461797" description="Phosphatidylserine decarboxylase beta chain" evidence="11">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023461796" description="Phosphatidylserine decarboxylase alpha chain" evidence="11">
        <location>
            <begin position="276"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="11">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="11">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="11">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity" evidence="11">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="11">
        <location>
            <begin position="275"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="11">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="310" mass="34633" checksum="656F2785F0A454CA" modified="2019-06-05" version="1">MLAHGANGVITPVPQSRFNVHGRLMQKQLFILSQYLLPHHLLSRLIGCIAECRVYWFKNVIIAWFVKRYQVDMSQVLVEDLTAYKNFNAFFTRALKYGARILDQTQGAVLSPTDGVISQLGSIENGRVFQAKGHSFNVLELLGGDLALATPFMGGDFATIYLSPKDYHRVHMPIAGTLRDMIYVPGRIFSVNQTTVKNVPKLFTRNERAICLFDTERGPMAIVLVGAMIVASIETVWAGLVTQPKRELKAFRYDQATQAPIYLEKGAELGRFKLGSTAIVLFSQDQVSWVEKLMTGTLVQMGQCIAFPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL05</accession>
    <name>A0A481QL05_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="15">UvrABC system protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">uvrA</name>
        <name evidence="15" type="ORF">C3B55_00306</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000288953">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="C:excinuclease repair complex"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="UvrA_DNA-bd"/>
    </dbReference>
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        <property type="entry name" value="UvrA_inter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0228">DNA excision</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="612"/>
            <end position="943"/>
        </location>
    </feature>
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        <location>
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            <end position="653"/>
        </location>
        <ligand>
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        </ligand>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="QAX81661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="948" mass="105814" checksum="BFD66950DC7D7931" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL38</accession>
    <name>A0A481QL38_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00244</name>
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    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00244"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="42" mass="4629" checksum="8FF45ADDF6956881" modified="2019-06-05" version="1">MTFVNKGGGINGEQVKTVTSEYAYKLKHHVAVGDRFTDNDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL39</accession>
    <name>A0A481QL39_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Apolipoprotein N-acyltransferase</fullName>
            <shortName evidence="9">ALP N-acyltransferase</shortName>
            <ecNumber evidence="9">2.3.1.269</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">lnt</name>
        <name evidence="11" type="ORF">C3B55_00336</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phospholipid dependent N-acylation of the N-terminal cysteine of apolipoprotein, the last step in lipoprotein maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a glycerophospholipid + N-terminal S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein] = a 2-acyl-sn-glycero-3-phospholipid + H(+) + N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48228"/>
            <dbReference type="Rhea" id="RHEA-COMP:14681"/>
            <dbReference type="Rhea" id="RHEA-COMP:14684"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136912"/>
            <dbReference type="ChEBI" id="CHEBI:140656"/>
            <dbReference type="ChEBI" id="CHEBI:140657"/>
            <dbReference type="ChEBI" id="CHEBI:140660"/>
            <dbReference type="EC" id="2.3.1.269"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; lipoprotein biosynthesis (N-acyl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CN hydrolase family. Apolipoprotein N-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.269" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00336"/>
    <dbReference type="UniPathway" id="UPA00666"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07571">
        <property type="entry name" value="ALP_N-acyl_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01148">
        <property type="entry name" value="Lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004563">
        <property type="entry name" value="Apolipo_AcylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045378">
        <property type="entry name" value="LNT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20154">
        <property type="entry name" value="LNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00546">
        <property type="entry name" value="lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="CN hydrolase" evidence="10">
        <location>
            <begin position="232"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="517" mass="57833" checksum="9AA365F84E5EB8FB" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL40</accession>
    <name>A0A481QL40_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="5">IGPD</shortName>
            <ecNumber evidence="5 6">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">hisB</name>
        <name evidence="7" type="ORF">C3B55_00159</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="5 6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00159"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="PTHR23133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4 5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="197" mass="22254" checksum="1CA64E8416A400F4" modified="2019-06-05" version="1">MVERKASVERKTLETNIRVSINLDGTGKSRFDIGIPFFEHMLNQVAYHGLIDLDIVSEGDLHIDDHHTVEDLGITLGQAFTNAIGDKKGIRRYGYAYVPLDEALSRVVIDFSGRPSLHMHVPYTRAIVGGFDVDLFREFFQGCVNYALFSLHIDNLRGTNTHHQIETVFKAFGRALRIAVELDDRMLRQIPSTKGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL48</accession>
    <name>A0A481QL48_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">C3B55_00255</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00255"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO" evidence="11">
        <location>
            <begin position="2"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="297" mass="33394" checksum="425190AB671BE5AB" modified="2019-06-05" version="1">MKTPKRIEPMIEDGMIDEVLRELMSGKEASVYVVRCGNELRCAKVYKKANKRSFRHMSKYQEGRKIRNSRLARAMAKGSKFGKQETEDAWQNAEVAALFRLASAGVRVPKPYDFLDGVLLMEFMTNEYGAAAPRLNDVAIEPDKAREYHAFLISQIVLMLCTGLVHGDLSEFNVLLTTTGPVIIDLPQVMDAVGNNYAFSILERDVGNISAYFGRFAPELKNTQYAKEMWALYAAGTLHPTSALTGKFDEPKELVNVCSVIREIEVVQLDKKRYEAMLAADDAPLGKKSKKPLPYWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL64</accession>
    <name>A0A481QL64_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">trpD</name>
        <name evidence="8" type="ORF">C3B55_00123</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + N-(5-phospho-beta-D-ribosyl)anthranilate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00123"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="PTHR43285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="1 5 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycos_trans_3N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_3" evidence="6">
        <location>
            <begin position="76"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="5">
        <location>
            <begin position="85"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="5">
        <location>
            <begin position="92"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="5">
        <location>
            <begin position="110"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="349" mass="36916" checksum="FBDD13566E3C8C78" modified="2019-06-05" version="1">MNIKTALSRIVSHLDLSTAEMSDVMSEILTGQCTDAQIGAFMIAMRMKCESIDEIVGAVLIMRKLADKVKLKTLESVVDVVGTGGDGANIFNVSTASSFVIAAAGCTVAKHGNRAVSSKSGSADLLEAAGIYLNLTPAQVARCIDSVGIGFMFAQSYHSGMKYAAGPRQDLGLRTIFNILGPLTNPAGVKHQVVGVFSQALCRLLAEVLQRLGSKHALVVHSKDGLDEFSLAVPTVVAELQNDQVTEYCIKPEDLGIKSQSLHGLSVESPAASLGLIRDALSRRKTKNGQKAADIIILNAGAALYAADYVYSLKDGVALAHDALQSGLAREKLEELGIFTAVLKTENNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL79</accession>
    <name>A0A481QL79_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="8">BPG-independent PGAM</shortName>
            <shortName evidence="8">Phosphoglyceromutase</shortName>
            <shortName evidence="8">iPGM</shortName>
            <ecNumber evidence="8">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 14" type="primary">gpmI</name>
        <name evidence="14" type="ORF">C3B55_00164</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the BPG-independent phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00164"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006007">
        <property type="term" value="P:glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16010">
        <property type="entry name" value="iPGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01038">
        <property type="entry name" value="GpmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011258">
        <property type="entry name" value="BPG-indep_PGM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036646">
        <property type="entry name" value="PGAM_B_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005995">
        <property type="entry name" value="Pgm_bpd_ind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637">
        <property type="entry name" value="PTHR31637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06415">
        <property type="entry name" value="iPGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001492">
        <property type="entry name" value="IPGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64158">
        <property type="entry name" value="SSF64158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01307">
        <property type="entry name" value="pgm_bpd_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 8 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="12">
        <location>
            <begin position="5"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="iPGM_N" evidence="13">
        <location>
            <begin position="83"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="154"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="258"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="8 9">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="507" mass="55529" checksum="D750DAC961D34448" modified="2019-06-05" version="1">MTTPKPLVLIILDGFGHSENHHYNAVYLAKTPVLDHLIATAPSGLLSSSGMDVGLPEGQVGNSEVGHMTLGTGRVVYQNFTRVTEAIHDGKFFENPIICAAVDKAVAAGKAVHVMGLLSDGGVHSHQNHLVAMAKLAFKRGTDKIYLHAFLDGRDTPPKSARSSIELLDTTFATLGKGHIASMIGRYFAMDRNNYWDRVAQAYNLIVDGQAEFNSATAQEGLQAAYVRGESDEFVKATTIGEPVQVENGDAVVFMNFRSDRARELSYIFVNTNFKNFERARQPKVEFVMLTKYATDIHAPAAFEPDSLKNVLGDYLSKNGKTQLRIAETEKYAHVTFFFSGGREEPFPGEERILIQSPKVATYDLQPEMSALEVTNKIINAIKYQHFDMIVVNYANSDMVGHSGNLKAAVKAVEFLDLCIGRIVDNLKKVGGEALITADHGNCERMSDKCTGQPHTAHTAEPVPFIYIGKRNLTVRKGGVLADVAPTILKLMGLKKPKEMTGTSILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL83</accession>
    <name>A0A481QL83_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="5">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Gamma-ECS</fullName>
            <shortName evidence="5">GCS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">gshA</name>
        <name evidence="8" type="ORF">C3B55_00145</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5 6">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00145"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00578">
        <property type="entry name" value="Glu_cys_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007370">
        <property type="entry name" value="Glu_cys_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006334">
        <property type="entry name" value="Glut_cys_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38761">
        <property type="entry name" value="PTHR38761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04262">
        <property type="entry name" value="Glu_cys_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01434">
        <property type="entry name" value="glu_cys_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glu_cys_ligase" evidence="7">
        <location>
            <begin position="9"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="527" mass="59706" checksum="811B106F2FC8C24D" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL99</accession>
    <name>A0A481QL99_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-type D-Ala-D-Ala carboxypeptidase</fullName>
            <ecNumber evidence="4">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">dacC</name>
        <name evidence="17" type="ORF">C3B55_00329</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S11 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00329"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015956">
        <property type="entry name" value="Peniciliin-bd_prot_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012907">
        <property type="entry name" value="Peptidase_S11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037167">
        <property type="entry name" value="Peptidase_S11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07943">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00936">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69189">
        <property type="entry name" value="SSF69189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 17" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019807321" description="Serine-type D-Ala-D-Ala carboxypeptidase" evidence="15">
        <location>
            <begin position="25"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="PBP5_C" evidence="16">
        <location>
            <begin position="275"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="12">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX81684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="385" mass="42345" checksum="DD0F3BEB4E3D3D9C" modified="2019-06-05" version="1">MNITTLAKHICMLCSLMITQLTWAAEMVPTFPQLAAKSWILMDAVSGNVLVEGNSDQRLPPASLTKLMTAYIATMEIIRGQIGENDKVTVSENAWRTVGSRMFIKIGSQVTVSDLLHGIIIQSGNDASVALSEHIAGSEDAFVDLMNDTAGDLGMNNSNFINTTGLPSQDHYSSAHDIAILARAIIYDDPVRYTIYSQKEFFWNNIKQLNRNLLLWRDKAVDGLKTGYTDEAGYCIASSAIHDGQRLIAVVFGANSEQARAVETQKLLTYGFHFFEAHTFYHKATVLSRAKVWNGAPNQVTAGLADNLILTMPKGQLNNLVTSITMNSQLIAPIAKGDVIGKVEVISNNKVVHSVDLIALDAIDKGSIFRCVWNKINLLFYDLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLD2</accession>
    <name>A0A481QLD2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Carbamoyl-phosphate synthase large chain</fullName>
            <ecNumber evidence="13">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 17" type="primary">carB</name>
        <name evidence="17" type="ORF">C3B55_00362</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 4 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="13"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00362"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_A">
        <property type="entry name" value="CPSase_L_chain_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_B">
        <property type="entry name" value="CPSase_L_chain_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CarbamoylP_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="170"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="715"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="16">
        <location>
            <begin position="973"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carboxyphosphate synthetic domain" evidence="13">
        <location>
            <begin position="1"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Oligomerization domain" evidence="13">
        <location>
            <begin position="441"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric domain" evidence="13">
        <location>
            <begin position="973"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="865"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="877"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="879"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX81716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="1110" mass="121679" checksum="E1B8B83D856D221C" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLE8</accession>
    <name>A0A481QLE8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
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            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000288953">
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="67" mass="7588" checksum="08EBEFCDF09446EB" modified="2019-06-05" version="1">MLILPINIVDNLSDVIEDINKDSFPHTEAIASKYYYSSIQCTLTEENYGSAMVIVSMLFADSYRCMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLF1</accession>
    <name>A0A481QLF1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
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            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00384"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="52" mass="6267" checksum="96118165BF8041E8" modified="2019-06-05" version="1">MVSMRCKQFAYTVCILMPHHRIDSQNYLTHIYTNQYKITLRSLLDARNVDYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLF2</accession>
    <name>A0A481QLF2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">elbB</name>
        <name evidence="3" type="ORF">C3B55_00044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Displays glyoxalase activity, catalyzing the conversion of glyoxal to glycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glyoxal + H2O = glycolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51672"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:34779"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026041">
        <property type="entry name" value="ElbB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10224:SF12">
        <property type="entry name" value="PTHR10224:SF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006320">
        <property type="entry name" value="Elb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="2">
        <location>
            <begin position="15"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="221" mass="23545" checksum="B524A14CC1356837" modified="2019-06-05" version="1">MSKNIAVILSGCGVYDGTEIHESVITLLCLDQHGAQVQCFAPDITQQHVINHLTGEKMPESRNVLVESARIVRGQVKDICEANANAFDALIMPGGFGAVTNLSNFAIEGAGCSVNPQILELVKAFAEARKPVGLICISPALAAKIYGPGVKCTIGNCPDTIATLDKMGATHCECTVENIVEDQARKLLSTPAYMLGKSISEVSSGINKLVERVLDLTYENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLF3</accession>
    <name>A0A481QLF3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylmuramoyl-L-alanine amidase</fullName>
            <ecNumber evidence="2">3.5.1.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">amiC</name>
        <name evidence="5" type="ORF">C3B55_00227</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.</text>
            <dbReference type="EC" id="3.5.1.28"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.5.1.28" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00227"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021731">
        <property type="entry name" value="AMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11741">
        <property type="entry name" value="AMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00646">
        <property type="entry name" value="Ami_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MurNAc-LAA" evidence="4">
        <location>
            <begin position="241"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="473" mass="51964" checksum="5F0E6DFCFA4D5C81" modified="2019-06-05" version="1">MYRFSIRILIKLMELGMRLRAMVVVVWVLLTVMTFSALAVSQVKDVRVWRAPHNMRLVFDLSGPVRYDVFTLISPDRLVVDIHGANLVAPLKVYALNTQIIAMRSFQRAPIDLRVVVDLKKSVTTKSFILAPNAKYGHRLVVDLFDNHAAAVPSALTNTPMPQVKLPLVLPVPTGKRDIIVVIDAGHGGEDPGASSPRGQYEKDVVLAISRKLQHQVNSMKGYRADLTRTSDYFIPLRGRTEIARKKNADLFVSIHADAAPSTAVYGASVFALSDRSATSETSRWLADNENRFDLISGTGNVSLDNKDKMLAGVLLDLSMTASITSSLNVGQKALSNIGRITSLHKRHVEQAGFMVLKSPDIPSILVEAGFISNSYEASKLASVSHQEALARSISNGIRQFFQQNPPPGTYIAWLRDSGKIVIDQLNYHLRSGDTMGHIASSSMTILRSTSNLKTNKLKVDQVLTIRGTELAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLH0</accession>
    <name>A0A481QLH0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative Fis-like DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fis</name>
        <name evidence="4" type="ORF">C3B55_00249</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory Fis family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00249"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005412">
        <property type="entry name" value="Fis_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002097">
        <property type="entry name" value="DNA-binding_Fis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01591">
        <property type="entry name" value="DNABINDNGFIS"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_8" evidence="3">
        <location>
            <begin position="62"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="105" mass="11839" checksum="8460A846AA528538" modified="2019-06-05" version="1">MMMIETLVSEITPVSDNVNLKQYLNTPNEEGQTLRRSVKKALHNYFTNLDGASVKDLYSLVLSEIEAPLLESVMDHVKGNQTKASSMLGLNRGTLRKKLKQYNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLH3</accession>
    <name>A0A481QLH3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00485"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008318">
        <property type="entry name" value="UCP030820"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06073">
        <property type="entry name" value="DUF934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030820">
        <property type="entry name" value="UCP030820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="164" mass="19041" checksum="10622CE8965A17FA" modified="2019-06-05" version="1">MQRIIKNNTVFNENWHLLPKGASFDSISSCDNLIVPLAMWCEYANTLKARYGSLGVWLDADEEAEVISCDVEQFQVIALNFPVFTDGRNYSNARLLRDRYAFKGELRAIGDILRDQLFYLHRCGFDAFALRADKDPYEALKSFKDFSVMYQSATYEPLPLFRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLI7</accession>
    <name>A0A481QLI7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein AmpE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00433"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031347">
        <property type="entry name" value="AmpE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17113">
        <property type="entry name" value="AmpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="278" mass="32130" checksum="D13A4C5BDE6807E1" modified="2019-06-05" version="1">MPFLVLILAVWMKKCLILRLWLQRDGLWLRELTKLELSTRMVKQPWLILILLVLLPVILLALLLSMLEPMAYGLLVFPVHLLVVIYSLGRGDLLADLRQFRNAFYCGDFQAFENVAKYDFKVASTSSEQLLKLVQGYLLWQAYQSFFAVIFWFFLFGPVTVLAYRLLALISEHSQNSLLTERTKQLRHVFDWLPVRMLSVSFALVGNFVAVSRVMQHELLNLDICAAQLIEKVGLVAIEIQSPLTEVDSINSLDRLWALLLRAAVLWYAWFAIWTVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLK0</accession>
    <name>A0A481QLK0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Toluene tolerance, Ttg2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00517"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008869">
        <property type="entry name" value="MlaC/ttg2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042245">
        <property type="entry name" value="Tgt2/MlaC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36573">
        <property type="entry name" value="PTHR36573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05494">
        <property type="entry name" value="MlaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004649">
        <property type="entry name" value="MlaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019726819" evidence="2">
        <location>
            <begin position="23"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="208" mass="23286" checksum="6B10452FC689EBF2" modified="2019-06-05" version="1">MISILKSGLLILLAVLPLTSKAAGSAHDLVQDITNKMLADLAAHKEQYKQNPTKFYDALNAIIGSVIDAEGISRSIMTVKYSCKATPAQMKTFQENFKKSLFQFYGNALLEYNDQRINITSAVDESINRTSVNMTVKANNGIIYPVQYTMEKINGEWKLRNVIINGINIGKLFRDQFVDAMQRNNNDLNKTINSWTSEIAKATAETKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLL1</accession>
    <name>A0A481QLL1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rpmJ</name>
        <name evidence="6" type="ORF">C3B55_00298</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00298"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_L36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_L36_sp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42888">
        <property type="entry name" value="PTHR42888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="SSF57840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00828">
        <property type="entry name" value="RIBOSOMAL_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="38" mass="4521" checksum="28A87A83C7D960DC" modified="2019-06-05" version="1">MKVRASVKKLCRNCKIIRRKNVVRVICGMEPRHKQRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLN2</accession>
    <name>A0A481QLN2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00480"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="120" mass="13715" checksum="A09370FBBFDBD84D" modified="2019-06-05" version="1">MSRLAEFRAVKKALQEQLAKLESLKKDAGLQKEIEFEEKLQDLMKYYSKNLCDIISILEPSYGKASTATIASKQRNIRVVKVYHNQHTGDLIETKDTNHRGLQAWKEQYGAAIVDSWLCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLP5</accession>
    <name>A0A481QLP5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
            <ecNumber evidence="9">2.7.8.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UDP-MurNAc-pentapeptide phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">mraY</name>
        <name evidence="10" type="ORF">C3B55_00579</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMP</text>
            <dbReference type="Rhea" id="RHEA:28386"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="EC" id="2.7.8.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyltransferase 4 family. MraY subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.13" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00579"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051992">
        <property type="term" value="F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06852">
        <property type="entry name" value="GT_MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00038">
        <property type="entry name" value="MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003524">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10555">
        <property type="entry name" value="MraY_sig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00445">
        <property type="entry name" value="mraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01347">
        <property type="entry name" value="MRAY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="360" mass="39496" checksum="2158FD23101410EA" modified="2019-06-05" version="1">MLLLLAEYLQQFYSGFAVFQYLTLRGILSVMTALSLSLFLGPWMIHTLQSLQISQSVRNDGPQSHLSKSGTPTMGGALILSSISTSTLLWVDLHNCYVWVVLLVTILFGVIGWFDDYCKVIEKNSRGLPSYWKYLWQSVFGLTAAIFLYVTAPSAVETTLIIPMFKDASTPLGIGFVVLTYFVIVGSSNAVNITDGLDGLAIMPAMMVGGALGIFCYLSGNIKFAEYLLIPYVPGAGELIVFCSALLGASLGFLWFNTYPAQVFMGDVGALALGAALGTIAVIVRQEIVLFIMGGVFVMETLSVIIQVLSFKLTARRVFRMAPIHHHFELKGWPEPRVIVRFWIINVILVLAGLATLKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLP8</accession>
    <name>A0A481QLP8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cobalamin-independent methionine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Methionine synthase, vitamin-B12 independent isozyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">metE</name>
        <name evidence="13" type="ORF">C3B55_00502</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-methionine + tetrahydropteroyltri-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21196"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58140"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
            <dbReference type="EC" id="2.1.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the vitamin-B12 independent methionine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.14" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00502"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cbl-indep_Met_Synth_N"/>
    </dbReference>
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        <property type="entry name" value="Cobalamin-indep_Met_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08267">
        <property type="entry name" value="Meth_synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000382">
        <property type="entry name" value="MeTrfase_B12_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="2"/>
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    <dbReference type="TIGRFAMs" id="TIGR01371">
        <property type="entry name" value="met_syn_B12ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4 10 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Meth_synt_1" evidence="12">
        <location>
            <begin position="4"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Meth_synt_2" evidence="11">
        <location>
            <begin position="433"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-methyltetrahydropteroyltri-L-glutamate binding" evidence="10">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-homocysteine binding" evidence="10">
        <location>
            <begin position="438"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-methionine binding" evidence="10">
        <location>
            <begin position="438"/>
            <end position="440"/>
        </location>
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    <feature type="region of interest" description="5-methyltetrahydropteroyltri-L-glutamate binding" evidence="10">
        <location>
            <begin position="522"/>
            <end position="523"/>
        </location>
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        <location>
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        </location>
    </feature>
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        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="491"/>
        </location>
    </feature>
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        <location>
            <position position="568"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
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        <location>
            <position position="606"/>
        </location>
    </feature>
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        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="648"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="650"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="672"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="733"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00002777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004681"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="QAX81838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="762" mass="86801" checksum="75E1B96139C5A52D" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-02-10" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLR8</accession>
    <name>A0A481QLR8_9PSED</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">C3B55_00206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
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        <lineage>
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            <taxon>Gammaproteobacteria</taxon>
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            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
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            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000288953">
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    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="471" mass="54599" checksum="7A70B369BA2B9803" modified="2019-06-05" version="1">MSELINRKQILLKRYRRNKCMGLLVSLLILICVGLLVVWWLPILLAVFTWLVHENWFVEYLFYSPKNDYQYKFTADTEQLGVTLDKGRLVLRQEFELIGDETLILAVRLKSSMVGHFIDPYVVLSYDDRQVFERGVNGLRYLNLTGQAQLLLSGQLLLRSRFCHVQGELVLSVFRYPDVRRQRMMVIAPHADDAELAAFGLYSQTVQPWIVTLTAGEIEAEHYQRMGLSKPEAARLKGRLRAWDSIVVPRWAGIRQEYLIQLGYFCLQLMAMKLEPDRAVVSLEAELNDIRVFRQFNQFLLPADTNGLATWSNLLADLRAIILLARPEVIVLPCSLLDSHPDHVCARQAVLEALQDLEWQPTILLGYANHLHDNNRWPMGDSGNGVALPPFFDTTYTLLPYCVSLSKQEQCNKAMSLGMMHDLQPRISFKRRVRRVIQRLFSGRAISPWGENDFFRKAVRRHELFWFVKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLR9</accession>
    <name>A0A481QLR9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH-quinone oxidoreductase subunit E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">NADH dehydrogenase I subunit E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">NDH-1 subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nuoE</name>
        <name evidence="10" type="ORF">C3B55_00444</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="9">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
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        <property type="protein sequence ID" value="QAX81787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
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        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="165" mass="18579" checksum="5EA7DE6D08A507F7" modified="2019-06-05" version="1">MNSTLFQTNNFILRKTERSAIEHILNHYDDYRAASIEALKIIQKERGWVPDGALYAISKILSITTSDVEGVATFYSQIFRQPVGRHIIRVCDSITCYIGGYESVISEIKNKHDIDLGQTTQDNRFTLLPVCCLGNCDKAPALMIDDDTFGDMRPAGVIQLLESYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLS3</accession>
    <name>A0A481QLS3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamate dehydrogenase</fullName>
            <ecNumber evidence="2">1.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gdhB</name>
        <name evidence="4" type="ORF">C3B55_00623</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamate + NAD(+) = 2-oxoglutarate + H(+) + NADH + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.2"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.4.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00623"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019551">
        <property type="term" value="P:glutamate catabolic process to 2-oxoglutarate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028971">
        <property type="entry name" value="NAD-GDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007780">
        <property type="entry name" value="NAD_Glu_DH_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43403">
        <property type="entry name" value="PTHR43403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05088">
        <property type="entry name" value="Bac_GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036761">
        <property type="entry name" value="GDH_Mll4104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="1622" mass="183426" checksum="0A1856341195B652" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLT0</accession>
    <name>A0A481QLT0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="6">RPPK</shortName>
            <ecNumber evidence="6">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="6">P-Rib-PP synthase</shortName>
            <shortName evidence="6">PRPP synthase</shortName>
            <shortName evidence="6">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">prs</name>
        <name evidence="8" type="ORF">C3B55_00403</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00403"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran_N" evidence="7">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="96"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="313" mass="34315" checksum="0AC165CB0CB807C3" modified="2019-06-05" version="1">MSKMMVFTGNANPDLVRRVVRHLHIPLGDISVGKFSDGEITAEINENVRGKDVFIIQPTCAPTNDNLMELVVMADSFRRSSATRITAVIPYFGYARQDRRPRSARVAISAKVVADMLTVVGIDRVLTVDLHADQIQGFFDIPVDNIYGSPVLMDDIEGQRFENLMIISPDIGGVVRARAVAKSLSVDLGIIDKRREKANQSEVMHIIGDVEGRICILIDDMVDTSGTLCHAATALKKHGAEKVFAYCTHPVLSGQAISNIENSMLDELIVTNTIPLSTAAQACSRIRQLDIAPIVAEAVRRISNEESISAMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLT1</accession>
    <name>A0A481QLT1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">dnaJ</name>
        <name evidence="12" type="ORF">C3B55_00359</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DnaJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00359"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0346">Stress response</keyword>
    <keyword evidence="5 8 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="5"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="11">
        <location>
            <begin position="133"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="8">
        <location>
            <begin position="146"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="8">
        <location>
            <begin position="163"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="8">
        <location>
            <begin position="185"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="8">
        <location>
            <begin position="199"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="9">
        <location>
            <begin position="133"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="374" mass="40832" checksum="E3600B65003420E6" modified="2019-06-05" version="1">MAKRDYYEVLGVERGSSEADLKKAYRRLAMKHHPDRNPDSKESEELFKEVNEAYECLSDPKKRAAYDQYGHVGVDPSMGGGGTGFSGQNFSDIFGDVFSDFFNSGRSAQRGGAQRGNDLRYTLELNLEEAVRGTTVNIRVPTLVSCNTCNGLGAKKGSSPITCSTCGGIGQVRMQQGFFSVQQTCPRCHGQGKIISDPCNSCHGEGRTEEYKTLSVSIPTGVDTGDRIRLSGEGEAGIQGGPMGDLYVMINVREHSIFRRDGKHLACEVPINFVDAALGGELEIPTLDGRVKLKIPEGTQTGKQFRIRGKGVAPVRGGDTGDLMCRVVVETPVNLGRRQRELLEEFRNSLKSDDSHSPKTTGFFDGVKRFFGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLW1</accession>
    <name>A0A481QLW1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Alkyl hydroperoxide reductase C</fullName>
            <ecNumber evidence="4 11">1.11.1.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 11">Peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ahpC</name>
        <name evidence="13" type="ORF">C3B55_00479</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>a hydroperoxide + H(+) + NADH = an alcohol + H2O + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:62628"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.11.1.26"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.26" evidence="4 11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00479"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102039">
        <property type="term" value="F:alkylhydroperoxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017559">
        <property type="entry name" value="AhpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF121">
        <property type="entry name" value="PTHR10681:SF121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03137">
        <property type="entry name" value="AhpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 11 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 11 13" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="2"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="10">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="187" mass="20504" checksum="52B7E3520A94CCE2" modified="2019-06-05" version="1">MSLINSQVKPFKATAFKNGSFVEVTDVNLKGKWSVVFFYPADFTFVCPTELEDLADNYAGFQKLGVEIYSISTDTHFAHAAWHKTSQAIGKIQYTMIGDPTLTISRSFDVLIEETGLAERGTFVINPEGQIKTVELSDGGVGRDTSELLRKIKAVQYVAAHPGEVCPARWKEGEATLAPSLDLVGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLX7</accession>
    <name>A0A481QLX7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lipoprotein-releasing system transmembrane protein LolC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">lolC_2</name>
        <name evidence="12" type="ORF">C3B55_00710</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC-4 integral membrane protein family. LolC/E subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00710"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011925">
        <property type="entry name" value="LolCE_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02212">
        <property type="entry name" value="lolCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="22"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="317"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="11">
        <location>
            <begin position="27"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="10">
        <location>
            <begin position="302"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="416" mass="45889" checksum="6C62AA4875A7BB70" modified="2019-06-05" version="1">MFRPLSVFIGMRYTRAKHRNHFLSFISIVSIIGLALGVAVMIVVLSVMNGFDHEMRTRVLGMVPHAIIESYEPISDWQSLADQVMKASKVMAVAPFTQIPGLLAYDGKVQKILLNGIEPSQESNVSIIDHCMLHGKLNDLVPGKFGIVIGDKAAVRFSVGIGDKLTFIAPEVIVTPAGMFPCMKRFTVVGIFHVGAAEIDGYLCLTNLIDLAHVHRWQSDQVQGLRLKFHSLFDAPLSALDIVKHLGESKYYAIDWTRTHGNLYQAIRMEKAVIGLLLLLIIAIAAFNIISMLVIIVNNKTSDIAILRTIGVMPRQIISIFIVEGTLIGMVGTLIGTVFGILCALNIRTTIAALERVIGYTFLNADIYFIDYVPSKVQTQDVFIVGCAALFLSFIATLYPAWRAACTQPSHALRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLY2</accession>
    <name>A0A481QLY2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">clpX</name>
        <name evidence="9" type="ORF">C3B55_00604</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ClpX-ClpP complex. Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the ClpX chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00604"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00175">
        <property type="entry name" value="ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004487">
        <property type="entry name" value="Clp_protease_ATP-bd_su_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046425">
        <property type="entry name" value="ClpX_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010603">
        <property type="entry name" value="Znf_CppX_C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038366">
        <property type="entry name" value="Znf_CppX_C4_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06689">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00994">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00382">
        <property type="entry name" value="clpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51902">
        <property type="entry name" value="CLPX_ZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ClpX-type ZB" evidence="8">
        <location>
            <begin position="4"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="122"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="427" mass="47219" checksum="5A59F929A65E843C" modified="2019-06-05" version="1">MTDTCNSEDNGKLLYCSFCGKSQHEVRKLIAGPSVFICDECVSLCNDIIREEVQETQAENDLHKLLSPKEISGILDQYVIGQERAKKVLAVAVYNHYKRLNQHDKKGDEVELGKSNILLIGPTGSGKTLLVETLARLLNVPFTIADATTLTEAGYVGEDVENIIQKLLQRCDYDVEKAQMGIIYIDEIDKISRKSENPSITRDVSGEGVQQALLKLIEGTIASVPPQGGRKHPQQEFLQVDTRNILFICGGAFSGLEKVIQNRSAHGGIGFSAEVRSKKEVKNIGESLRQVEPDDLVKFGLIPEFIGRLPVLATLDELDESALIQILTEPKNALTKQYARLFEMEGVDLEFRTDALKSIAKWALKRKTGARGLRSILEGVLLDTMYEIPSQSEVSKVVIDESLIEGKSKPLYIYENSQSNAKAAPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM48</accession>
    <name>A0A481QM48_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">50S ribosomal protein L25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">General stress protein CTC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">rplY</name>
        <name evidence="6" type="synonym">ctc</name>
        <name evidence="8" type="ORF">C3B55_00402</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA. Binds to the 5S rRNA independently of L5 and L18.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00402"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00495">
        <property type="entry name" value="Ribosomal_L25_TL5_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01334">
        <property type="entry name" value="Ribosomal_L25_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029751">
        <property type="entry name" value="Ribosomal_L25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020057">
        <property type="entry name" value="Ribosomal_L25_b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037121">
        <property type="entry name" value="Ribosomal_L25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001021">
        <property type="entry name" value="Ribosomal_L25_long"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01386">
        <property type="entry name" value="Ribosomal_L25p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14693">
        <property type="entry name" value="Ribosomal_TL5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00731">
        <property type="entry name" value="bL25_bact_ctc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_TL5_C" evidence="7">
        <location>
            <begin position="102"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="200" mass="21791" checksum="4F811F816E6FDCD3" modified="2019-06-05" version="1">MNDFNLNAEARFNLGKGASRRMRRLANLVPAVVYGGNKASKSISIPTKEITKLLINHAAYSHVIELNIGGIKQTVIIKALQRHPAKDQVLHVDFVRVIADQKLTAIVLVRFIGEATIIKKGGEISHVTSEIEVSCLPKDLPEFIEVDLTNAKIGDIIHLSDLTVPRGIEFVALAHGDDKAIVNIHAPRVVTLEATEGATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM53</accession>
    <name>A0A481QM53_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold shock protein CapB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">capB_2</name>
        <name evidence="4" type="ORF">C3B55_00793</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00793"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="4"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="70" mass="7700" checksum="E71AB42A05005259" modified="2019-06-05" version="1">MSNRQTGTVKWFNDEKGFGFITPQGGGNDLFVHFKAIESEGFKSLKEGQTVSFVAEKGQKGMQAAQVRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM55</accession>
    <name>A0A481QM55_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ferrochelatase</fullName>
            <ecNumber evidence="7">4.99.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Heme synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Protoheme ferro-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">hemH</name>
        <name evidence="9" type="ORF">C3B55_00413</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 H(+) + heme b = Fe(2+) + protoporphyrin IX</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.99.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Fe-coproporphyrin III + 2 H(+) = coproporphyrin III + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:49572"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:68438"/>
            <dbReference type="ChEBI" id="CHEBI:131725"/>
            <dbReference type="EC" id="4.99.1.9"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:49574"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00413"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="PTHR11108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="2 7" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="342" mass="38588" checksum="0FE3B3B48A38DABB" modified="2019-06-05" version="1">MINQSLLLVNLGSPASASVTDVRSYLNQFLMDPYVIDLPWPLRRLLVSLIIIKRQHQSAHAYASIWWDEGSPLVVLGHRLQQQMTKQWTHGPVELAMRYGKPSIEAALMRLVAQGMQKVTLVPLYPQFANSTVTTVIEKARRVVRDKKLNVQFSIVHSFYDQPEYLDVLVASVKPYMKQDFDHLLLSFHGLPKHHLRKFYANSGHCFKNVDCCKSASLKVLTTCYRAQCFSVARNLASRIGLSEGTWSVAFQSRIGRAKWIEPNTKDSLEALACQGVKKLLVMCPAFVADCIETLEEIGNRGLEQFREAGGEELILVPCLNDNIQWAVALNTLCERATTISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM77</accession>
    <name>A0A481QM77_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH-quinone oxidoreductase subunit B</fullName>
            <ecNumber evidence="2">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">NADH dehydrogenase I subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">NDH-1 subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 5" type="primary">nuoB</name>
        <name evidence="5" type="ORF">C3B55_00446</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00446"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="1 2 3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0874">Quinone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="4">
        <location>
            <begin position="86"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="224" mass="25463" checksum="523F5465BECB909D" modified="2019-06-05" version="1">MQYNLTKIDSNALNNKHPIDKNETISDPLKDQIYKNIYMGKLKDALSNAVNWGRKNSIWPYNFGLSCCYVEMTTAFTAPHDIARFGAEVIRASPRQADLMVIAGTCFIKMAPIIQRLYEQMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYVPGCPPRPEAFLQGLILLQESISKECRQLSWIVGDQGVYRAKMPSQKEQYREQRIQVTNLRNPDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM79</accession>
    <name>A0A481QM79_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-oxoacyl-[acyl-carrier-protein] synthase 3</fullName>
            <ecNumber evidence="4">2.3.1.180</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fabH</name>
        <name evidence="4" type="ORF">C3B55_00830</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.180" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00830"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033818">
        <property type="term" value="F:beta-ketoacyl-acyl-carrier-protein synthase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACP_syn_III" evidence="3">
        <location>
            <begin position="108"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="ACP_syn_III_C" evidence="2">
        <location>
            <begin position="221"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="309" mass="33266" checksum="A472A935AF043CE0" modified="2019-06-05" version="1">MIGIKSIASYVPVDGVDNYAQGVKFSKNEDFIISKIGSAFLPRKAATQETSDLCVEAINALFINNPDLKREAIDALIVVTQNGDEEGLPHTTAIVQAKLGLPTNVAAFDISLGCSGYVYGIYAMKGFMEATGLKNGLLITADPYSKIINPEDRNTTMLFGDAATATWMGESATWLLGKSRFGTDGSGAPYLKVSDGVFFMNGRQVFNFALLKVPAHLHELLNDSDLKADKIDAFCIHQGSAAIVDAVARRFEGVPVEKFIKDMAGTGNTVSSSIPLLLEKHMIDGDWKRIAISGFGVGLSWGSAILYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM94</accession>
    <name>A0A481QM94_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00606"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="90" mass="10695" checksum="DCD0D0048A9F02FD" modified="2019-06-05" version="1">MTFYVNVKCCKMLSLFRLENCVNFFKETQSEPDYQSGICTNHILINMPMRNHCYRQKSHSIQTSAYRYLNLILILLLYNFFKPSTGRSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMA3</accession>
    <name>A0A481QMA3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Succinate dehydrogenase iron-sulfur subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">sdhB</name>
        <name evidence="11" type="ORF">C3B55_00662</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00662"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="139"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="238" mass="26886" checksum="3BF9D39B83C51A39" modified="2019-06-05" version="1">MVTSMLSVSVYRYNPDQESAPFMQEFQVDTCGKDLMVLDVLAFIKEQDESFSYRRSCREGVCGSDGMNINGKNGLACIMPLSTVVKRNKLILRPLPSLPVIRDLVVDMSIFYKQYEKVKPFLQNDTPTPAIERLQLPEERKKLDGLYECILCACCSTSCPSFWWNPDKFLGPAALLQSYRFLADSRDTKTSERLASLDDPFSVFRCRGIMNCVHVCPKGLNPTKAIGQIRNMLLQSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMB4</accession>
    <name>A0A481QMB4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Poly(A) polymerase I</fullName>
            <shortName evidence="5">PAP I</shortName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 11" type="primary">pcnB</name>
        <name evidence="11" type="ORF">C3B55_00382</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00382"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004652">
        <property type="term" value="F:polynucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006378">
        <property type="term" value="P:mRNA polyadenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00957">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010206">
        <property type="entry name" value="PolA_pol_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025866">
        <property type="entry name" value="PolyA_pol_arg_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12626">
        <property type="entry name" value="PolyA_pol_arg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01942">
        <property type="entry name" value="pcnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PolyA_pol" evidence="8">
        <location>
            <begin position="56"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PolyA_pol_RNAbd" evidence="10">
        <location>
            <begin position="216"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="PolyA_pol_arg_C" evidence="9">
        <location>
            <begin position="332"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="441"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="467" mass="54140" checksum="784347B5931B2F20" modified="2019-06-05" version="1">MLKKLFQSFRYPLRYTQHIQGTPKVLNSDQHSLERAKFSRYAVNIIERLQNAGYQAYLVGGCVRDMLLNITPKDFDVATSATPEQIRNEFRNARIIGRRFKLVHIHFGREIIEVATFRAHHPQNYEAANTNQSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSNHILDYDNGIHDIRNNLICLIGDPMQRYQEDPVRMLRAVRFAAKLNFGIEKHTAAPIHNLAPMLREIPSARLFEEVLKMFLSGYAANTFKILVDLQLFDPLFRASAEALKYNPIYTHTLISEALIRTDLRIKQNKSVTPAFLFAALLWPALLMRALRLQERGMLPKLAMQEAAHKLIIEQCQHITIPKRFTMLIREIWNIQERMLYRSGKRADLLLNNPRFRASYDFLLLRESAGEQTGGLGEWWTNYQNANDSQRRNMIHNLDSKRDRVGDDLKKRCSNNTKRRHSSINAAGAASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMC2</accession>
    <name>A0A481QMC2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Adenosine 5'-phosphosulfate reductase</fullName>
            <shortName evidence="10">APS reductase</shortName>
            <ecNumber evidence="6 10">1.8.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">5'-adenylylsulfate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Thioredoxin-dependent 5'-adenylylsulfate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">cysH</name>
        <name evidence="13" type="ORF">C3B55_00862</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 10">Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 10">
            <text>[thioredoxin]-disulfide + AMP + 2 H(+) + sulfite = [thioredoxin]-dithiol + adenosine 5'-phosphosulfate</text>
            <dbReference type="Rhea" id="RHEA:21976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.8.4.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 10">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the PAPS reductase family. CysH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.10" evidence="6 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00862"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043866">
        <property type="term" value="F:adenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004604">
        <property type="term" value="F:phosphoadenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00063">
        <property type="entry name" value="CysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011798">
        <property type="entry name" value="APS_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004511">
        <property type="entry name" value="PAPS/APS_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02055">
        <property type="entry name" value="APS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00434">
        <property type="entry name" value="cysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAPS_reduct" evidence="12">
        <location>
            <begin position="96"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; cysteine thiosulfonate intermediate" evidence="10">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX82172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="306" mass="35564" checksum="DF41E0BAAAAC1E7E" modified="2019-06-05" version="1">MNQHYPSKSRQTLSKRTPMRLSKQQNYQVAAIETMCHNAGQILISLLSYKALSTTDLQENFLMNQTFNVAKLAKIYANKSAHDILKLAFHQFGDGLWVSFSGAEDVVLIDIAWKLNKNVKVFSLDTGRLYPETYQFIEQVREFYKIDIELISPDHVKLEPFIKEKGLFSFYKDRHDECCGIRKIEPLRRKLSGVSAWVTGQRSDQSPSTRSKVAALEVDANFSTPKRTLYKFNPLIQMASEEAWNYIRMLELPYNSLHKRGFISIGCEPCTRPVLPNQHEREGRWWWEEDTHKECGLHTRNIISKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMC7</accession>
    <name>A0A481QMC7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tail-specific protease</fullName>
            <ecNumber evidence="8">3.4.21.102</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">prc</name>
        <name evidence="8" type="ORF">C3B55_00636</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase S41A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.102" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00636"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07560">
        <property type="entry name" value="Peptidase_S41_CPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004447">
        <property type="entry name" value="Peptidase_S41A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020992">
        <property type="entry name" value="Tail_Prtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040573">
        <property type="entry name" value="TSP_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11818">
        <property type="entry name" value="DUF3340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17804">
        <property type="entry name" value="TSP_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00225">
        <property type="entry name" value="prc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019775991" evidence="6">
        <location>
            <begin position="22"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="240"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="638"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="608"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="693" mass="78695" checksum="037554BF4136A4A6" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QME1</accession>
    <name>A0A481QME1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA polymerase III subunit delta</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">holB</name>
        <name evidence="4" type="ORF">C3B55_00873</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00873"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004622">
        <property type="entry name" value="DNA_pol_HolB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00678">
        <property type="entry name" value="holB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="1 4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="329" mass="37292" checksum="4BE9272DFF559D74" modified="2019-06-05" version="1">MVEAYPWQDSAWKRLTSRNNYAHAYLLHGPIGIGKRALSERFMASLLCHGQINLPACGECKSCLLLKADSHPDIYLLEPEDADKAIKVDQVRNLVSFVAQTAQMGWRKVVLIEPVEAMNINAANALLKSLEEPSGNTVFLLVSHQFSRLLPTIRSRCIQQACQLPSKAMSLQWLAKVLPECSEDQRVELLTLVSGSPLAAVKMHAQGVCEQRILVVDGVKKLLKRELFATQLAESTWKDIPLLLLFDWFYDWSSLIIRYQLTHDKNYLGLSDMRKVVQYLSQKSAHGKVLNIQDWILAQRQKVLDKANLNRVLLLEALLVQWVSLPGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QME2</accession>
    <name>A0A481QME2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA-dihydrouridine(20/20a) synthase</fullName>
            <ecNumber evidence="6">1.3.1.91</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">U20-specific dihydrouridine synthase</fullName>
            <shortName evidence="6">U20-specific Dus</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA-dihydrouridine synthase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dus</name>
        <name evidence="6" type="synonym">dusA</name>
        <name evidence="10" type="ORF">C3B55_00704</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouridine(20) in tRNA + NAD(+) = H(+) + NADH + uridine(20) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:53340"/>
            <dbReference type="Rhea" id="RHEA-COMP:13533"/>
            <dbReference type="Rhea" id="RHEA-COMP:13534"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.91"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouridine(20) in tRNA + NADP(+) = H(+) + NADPH + uridine(20) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:53336"/>
            <dbReference type="Rhea" id="RHEA-COMP:13533"/>
            <dbReference type="Rhea" id="RHEA-COMP:13534"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.91"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouridine(20a) in tRNA + NAD(+) = H(+) + NADH + uridine(20a) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:53348"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouridine(20a) in tRNA + NADP(+) = H(+) + NADPH + uridine(20a) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:53344"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Dus family. DusA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.91" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00704"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102264">
        <property type="term" value="F:tRNA-dihydrouridine20 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02041">
        <property type="entry name" value="DusA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004653">
        <property type="entry name" value="DusA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42907">
        <property type="entry name" value="PTHR42907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00742">
        <property type="entry name" value="yjbN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="5 6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Dus" evidence="9">
        <location>
            <begin position="11"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6 8">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="229"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="site" description="Interacts with tRNA" evidence="6">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with tRNA; defines subfamily-specific binding signature" evidence="6">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with tRNA" evidence="6">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with tRNA; defines subfamily-specific binding signature" evidence="6">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with tRNA; defines subfamily-specific binding signature" evidence="6">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="325" mass="36273" checksum="EC4E38A050318029" modified="2019-06-05" version="1">MHKTTFHRFSVAPMMDLTDSYCRFFLRLLSKYTWIYTEMVTTSAILYGKHGCLLHTQEAENPIALQLGGSIPADLATCARMAEAAGYNEVNLNVGCPSSRVQNNMIGAILMAHPALVADCVKAMRDAASIPVTVKHRIGISERDSYAELCDFVGTVKGAGCASFMVHARLARLDGLSPKENRDIPPLRYDVVSQLKQDFPKLEFILNGGIKTLKQCHEHLQTFDGVMLGRGVYYNPYLLAEVDQQLFGSETPVISRAQLFEQLQPYIAEQLATRGTVHHIMRHILWLGTRFPGARKFRQLLSVDIHKISDLLVLLDQAGELLKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QME5</accession>
    <name>A0A481QME5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase cytochrome b556 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sdhC</name>
        <name evidence="14" type="ORF">C3B55_00665</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Membrane-anchoring subunit of succinate dehydrogenase (SDH).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="12">The heme is bound between the two transmembrane subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, plus two membrane-anchoring proteins, SdhC and SdhD. The complex can form homotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome b560 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00665"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045281">
        <property type="term" value="C:succinate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018495">
        <property type="entry name" value="Succ_DH_cyt_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="PTHR10978"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000178">
        <property type="entry name" value="SDH_cyt_b560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01001">
        <property type="entry name" value="SDH_CYT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <note>ligand shared with second transmembrane subunit</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000178-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="128" mass="13934" checksum="E9115B656F473F0F" modified="2019-06-05" version="1">MKKAVNNQRPVNLDLRTIKLPITGVTSFLYRISGIILFLGLGIMLYVLSRSLDSEGGYVAVKACLTSLPAKFIAWGLISALLYHLIAGVRHLIMDMGIGETLEGGRLGSKIIIGISLVLIILVGVWIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMF0</accession>
    <name>A0A481QMF0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Signal peptide peptidase SppA</fullName>
            <ecNumber evidence="3">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sppA</name>
        <name evidence="3" type="ORF">C3B55_00882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.21.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00882"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S49" evidence="2">
        <location>
            <begin position="141"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="327" mass="36078" checksum="E5D78C67603A0E8D" modified="2019-06-05" version="1">MSDDWGALKKTESANDKSWKLLEKTILASVNEQRRARYWGIFFKLLTFAYLIAILALFNSLIEIEKSGSRSNNYIALIEVRGMIADKESASADNIVSSLRAAFEDSKVKGIIMRINSPGGSPVQSGYVYDEIRRLRTLHPETKLYAVISDLGASGAYYIASAADQIYADKASLVGSIGITAVGYGFVGTMEKLGVERRTYISGEHKSFLDPFQPQKADETQFWQSVLDTAHHQFIASVKQGRGDRLQDKDHPELFSGLVWSGEQALPLGLIDGLGSVSSVAWDVIGEKDLVDFTVQESPLDRFSKKLGASVIDKLALYLGFQGQTFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMF9</accession>
    <name>A0A481QMF9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Enoyl-[acyl-carrier-protein] reductase [NADH]</fullName>
            <shortName evidence="9">ENR</shortName>
            <ecNumber evidence="9">1.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fabV</name>
        <name evidence="12" type="ORF">C3B55_00702</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the final reduction of the elongation cycle of fatty acid synthesis (FAS II). Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2,3-saturated acyl-CoA + NAD(+) = a (2E)-enoyl-CoA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:18177"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.1.44"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TER reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.9" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00702"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016631">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050343">
        <property type="term" value="F:trans-2-enoyl-CoA reductase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01838">
        <property type="entry name" value="FabV_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024906">
        <property type="entry name" value="Eno_Rdtase_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024910">
        <property type="entry name" value="Enoyl-CoA_Rdtase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010758">
        <property type="entry name" value="Trans-2-enoyl-CoA_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37480">
        <property type="entry name" value="PTHR37480"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07055">
        <property type="entry name" value="Eno-Rase_FAD_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12242">
        <property type="entry name" value="Eno-Rase_NADH_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12241">
        <property type="entry name" value="Enoyl_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 9" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl_reductase" evidence="11">
        <location>
            <begin position="82"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Eno-Rase_FAD_bd" evidence="10">
        <location>
            <begin position="322"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="48"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="111"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="272"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in discriminating NADH against NADPH" evidence="9">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="395" mass="43056" checksum="42921174AF9AAFAC" modified="2019-06-05" version="1">MIIKPRVRGFICVTAHPVGCEANVKNQIDYVIQSATISGGPKKVLVLGASTGYGLAARISVAFGCGADTLGVFFEKEGEVGKPSSAGWYNSAAFEKFAVKKGLYAKSINGDAFSDEIKRLTIETIKKDFGKIDLIIYSVAAPRRTDRQGLVYNATLKPIGKTVIIRGINTDTGVVIDTILKPATQEEIDGTVKVMGGEDWQLWIDAMRDKNVLAEGVKTTAFTYLGEKLTQDIYWNGSIGKAKQDLDKKVVTIRDNLAILNGDARVSVLKAVVTQASAAIPIMPLYLSLLFKVMKEHGTHEGCIEQVYGLLKNSLYGSEPKTDSVGRLRADLAELEPKVQNAVATLWTQVTNDNINEISDFSGYKSEFLRLFGFGIDGIDYEVDVNQTVKINSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMG4</accession>
    <name>A0A481QMG4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Undecaprenyl phosphate N,N'-diacetylbacillosamine 1-phosphate transferase</fullName>
            <ecNumber evidence="4">2.7.8.36</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pglC</name>
        <name evidence="4" type="ORF">C3B55_00686</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.36" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00686"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102334">
        <property type="term" value="F:N,N'-diacetylbacilliosaminyl-1-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="3">
        <location>
            <begin position="2"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="186" mass="21036" checksum="2D4147623CDFE915" modified="2019-06-05" version="1">MKRFFDFILSLFALIILVVPIFLITIAVKLTSKGPALYWSNRIGMNNMPFKMPKFRSMHEGTPIVATHLLLNSSKYLTPVGNFLRKSSLDELPQLWSIIVGDMSLVGPRPALFNQFDLIELRTGKNVHRIRPGLTGLAQISGRDKLLITAKVDYDAYYAKNISIIFDIKILILTTINVVKRVGVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMI1</accession>
    <name>A0A481QMI1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="5">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">surE</name>
        <name evidence="7" type="ORF">C3B55_00748</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="5">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00748"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SurE" evidence="6">
        <location>
            <begin position="3"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="253" mass="27120" checksum="FDDFECDAB311FE83" modified="2019-06-05" version="1">MHILISNDDGVTAPGLAALYTALQDYAECVVVAPHQDKSGASSSMTLNRPLRSQVLANGFISVNGTPVDCVYLAINSLLNQEPDLVVSGINLGANLGDDVLYSGTVAAALEGCILGRTSFAFSFASRQLENLSTATYFARKLVVAYGSLDLPQHTVLNVNIPNMPLNYIRGIQLTRLGHRSRALAPLKVVDQYGKEGYWIATAGDAKDCSPGTDFYAIMQGYVSITPLQLDRTFSDAFSCLESWLKGAAFNGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMJ1</accession>
    <name>A0A481QMJ1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00488"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="82"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="254" mass="28653" checksum="CA6F20278560F45F" modified="2019-06-05" version="1">MTDKAFAQVNPEWLALISAAHEWLAGPIGKFLLDEERRMLEDELSRFFGSYLVHYGPSSQTLPVTAQVQHNVYLGAPLPGVEIVCEEQAWPLRENAADVVLLQHGLDFCISPHGLLREAASSVRPGGHLLIIGINPWSSWGLRHLFAHDGMRQARCISPSRVGDWLNLLNFALEKCRFGCYRPPLESTKWQYRLVGWERRLSVWQLCGGGFYLLVARKIGGGLRPVHQVLRQPIANLVPILMPIPKVNRKQNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMJ6</accession>
    <name>A0A481QMJ6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Co-chaperonin GroES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">10 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Chaperonin-10</fullName>
            <shortName evidence="3">Cpn10</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">groS</name>
        <name evidence="3" type="synonym">groES</name>
        <name evidence="5" type="ORF">C3B55_00929</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00929"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="PTHR10772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="98" mass="10319" checksum="9B74791B0B5F6703" modified="2019-06-05" version="1">MSKLRPLHDRVVIRRSEEEKKTSGGIVLPGSAAEKANHGVIVAAGPGKTLENGEVRALAVKVGDKVVFGPYSGSNTVKVDGEDLLIMVENEILAVLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMK2</accession>
    <name>A0A481QMK2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Adenylate kinase</fullName>
            <shortName evidence="6">AK</shortName>
            <ecNumber evidence="6 8">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">adk</name>
        <name evidence="10" type="ORF">C3B55_00498</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="6 8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00498"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="PTHR23359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ADK_lid" evidence="9">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="6">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="6">
        <location>
            <begin position="122"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="132"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="215" mass="23752" checksum="B1DD6745D9423E76" modified="2019-06-05" version="1">MRIMLMGAPGAGKGTQAKFITEKFSIPQVSTGDILRAAVTTGTEIGLTVKNMIESGGLVSDNTIINLVQERISREDCKNGFLFDGFPRTVPQAKAMIKVGIELDAIVEIAVEDKEIIQRIAGRRVHETSGRVYHTIYNPPKVEGKDDVTGENLVQRKDDTEKTIRYRLSVYYSQTKPLVDFYHRLSVAHGKPKYSQILGVGSVEIIASKMLQALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QML6</accession>
    <name>A0A481QML6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(R)-stereoselective amidase</fullName>
            <ecNumber evidence="2">3.5.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ramA</name>
        <name evidence="2" type="ORF">C3B55_00950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.100" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00950"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="275" mass="31603" checksum="A6BD31B13E133767" modified="2019-06-05" version="1">MQEYAAISSRKNMHDLSTLPNLNIALVQTILMWHDRQANLKHFELLLEQTKDADLIVLPEMFTTGFSMASKTLAEPENGPTYQWLQVQAAKYNAVITGSVIIHVADGSHRNRLLWAQPNGEVLHYDKRHLFRMAGELNYYTPGEYQMLFELNGWRIRPLICYDLRFPVWSYDIHHTDLLLYIANWPGVRRHHWNRLLPARAIENLCYVAALNRVGTDGNGLTYTGDSQILDFQGDTLLNAGNAEVVFSSCLNAVELAKYRIRFPVKLDADKFQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMM9</accession>
    <name>A0A481QMM9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-3-O-acylglucosamine N-acyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.191</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">lpxD</name>
        <name evidence="8" type="ORF">C3B55_00761</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a (3R)-hydroxyacyl-[ACP] + a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:53836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="ChEBI" id="CHEBI:137740"/>
            <dbReference type="ChEBI" id="CHEBI:137748"/>
            <dbReference type="EC" id="2.3.1.191"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the transferase hexapeptide repeat family. LpxD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.191" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00761"/>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03352">
        <property type="entry name" value="LbH_LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00523">
        <property type="entry name" value="LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007691">
        <property type="entry name" value="LpxD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020573">
        <property type="entry name" value="UDP_GlcNAc_AcTrfase_non-rep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43378">
        <property type="entry name" value="PTHR43378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04613">
        <property type="entry name" value="LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01853">
        <property type="entry name" value="lipid_A_lpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 6" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="LpxD" evidence="7">
        <location>
            <begin position="25"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="351" mass="36622" checksum="17154749611E6F6A" modified="2019-06-05" version="1">MTATIKLGKLAEFLGATLCGSKEKEITGLATLQEAGPAQLSFLANPQYRKYLVGSQAAAVLLKAVDADGFAGATLVVADPYLAYARVSHLFDPKPKAARGIHPSAVIADDAQVDLTVSIGAFTVIESSARISAGVTVGAHCFIGARCEIGVDGWLAPRVTLYHDVRIGERVVIQSGAVIGGEGFGFANYKGIWHKIAQVGGVLIGDDVEIGVNTAVDRGALADTVIGNGVKIDNQIQIAHNVQIGDHTAMAACVGISGSTRIGKHCMLAGGVGLVGHIDICDNVFITGMTMVTHSITEPGTYSSGTAMQTAAEWRKSAARLRKIDSMARRLRQVEKRVEDVTPSGNASSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMP2</accession>
    <name>A0A481QMP2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cell division protein ZapE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Z ring-associated protein ZapE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">zapE</name>
        <name evidence="4" type="ORF">C3B55_00540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Reduces the stability of FtsZ polymers in the presence of ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with FtsZ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AFG1 ATPase family. ZapE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00540"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01919">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030870">
        <property type="entry name" value="ZapE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="PTHR12169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="66"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="364" mass="42606" checksum="5ADDB24807777854" modified="2019-06-05" version="1">MTPIERYQSDLKRPDFFHDAGQENAVRHLQRLYDNLVVDSQNKPWMPSKLFSKKYHAPIKGLYFWGGVGRGKTYLVDTFFEVLPFKEKVHTHFHGFMKRVHEEMKTLSGVKNPLTIIAKQFSKEARVICFDEFFISDITDAMIIGTIMEELFKNGVTLVVTSNIMPDILYKDGLQRTRFLPTIALIKQNTEIINVDSGVDYRLRNLEQVKLFYFPLNKAAHESLKKSFHTLTLECTKTVKNDKLIIENREIIARRTCSNVSWFEFRQLCDGPRSQNDYIELGKIFHAVILSGVEQMSITTDDIARRFINMVDEFYNRNVKLIISAEVELKELYTGGRLNFEFQRTLSRLLEMQSNEFLLRRHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMV4</accession>
    <name>A0A481QMV4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADPH-dependent 7-cyano-7-deazaguanine reductase</fullName>
            <ecNumber evidence="2">1.7.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">7-cyano-7-carbaguanine reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">NADPH-dependent nitrile oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">PreQ(0) reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 4" type="primary">queF</name>
        <name evidence="4" type="ORF">C3B55_00707</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-deazaguanine + 3 H(+) + 2 NADPH</text>
            <dbReference type="Rhea" id="RHEA:13409"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58703"/>
            <dbReference type="EC" id="1.7.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.7.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00707"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046857">
        <property type="term" value="F:oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033739">
        <property type="term" value="F:preQ1 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00817">
        <property type="entry name" value="QueF_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029500">
        <property type="entry name" value="QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029139">
        <property type="entry name" value="QueF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016428">
        <property type="entry name" value="QueF_type2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14489">
        <property type="entry name" value="QueF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14819">
        <property type="entry name" value="QueF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004750">
        <property type="entry name" value="Nitrile_oxidored_YqcD_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03138">
        <property type="entry name" value="QueF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="QueF_N" evidence="3">
        <location>
            <begin position="16"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="83"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="223"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimide intermediate" evidence="2">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="85"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="252"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="276" mass="31138" checksum="28D42A0133072AD1" modified="2019-06-05" version="1">MHIVARHSPLGKSSEYISTYAPSLLFPISRSIRWAELGLTIETLPYIGVDFWNCFELSWLLPSGKPVVAIGEFSIPANSPNIIESKSFKLYLNSLNQTVFINTQSLETTLRTDLSTVAGTPMRVRIRALAEITSEGIQALPGVCIDDLDISISKYKHPCPELLRCDDSRTTEESVHSHLLKSNCPVTGQPDWGSVVVTYSGNVLNHASLLEYIVSFRQHSDFHEQCIDRIFLDLQHLLQPKKLTVYARYVRRGGLDINPYRSTEAMQFQNSRLARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN61</accession>
    <name>A0A481QN61_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">C3B55_00927</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00927"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010538">
        <property type="entry name" value="DHOR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06537">
        <property type="entry name" value="DHOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028099">
        <property type="entry name" value="DUF1111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019810905" evidence="5">
        <location>
            <begin position="20"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="343"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="475" mass="52334" checksum="602997571BFF90BE" modified="2019-06-05" version="1">MYCSFFRLSITLVVLSLIACNDVPRFTKAEPSESWAGGTTTVNKRDQNAFSLPSANLTPTRRLDFSVGNSFFRSPWVIAPSTTTARDGLGPLFNTNACQNCHIKDGRGHPPLPNALNAVSMLVRLSIPNTLAHTKIVEQIGVVPEPVYGGQLQDMAIPGVIPEGKVRVNYSPVNVTFKDSFVVELRKPHLQITHLGYGQMHQDTLFSARIAPPMIGLGLLEAISDTDILRNTDAKTADKQATIGRANWVWDYAQHKTVLGRFGWKAGQPNLNQQNAHAFSGDMGLTTSMRPFDDCTEAQGTCKQAPNGNNANDEPEVSDNILRLVLFYTRNLAVPMHRDSNTAQVLAGKNLFYQAGCQGCHKPKFTTSATASEPELANQLIYPYSDLLLHDMGPGLADYRSEFKAGDRDWRTPPLWGIGLTQIVNDQAKFLHDGRARNLLEAVLWHGGEAQAAQQHVLSFNIEQRTELLAFLNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN62</accession>
    <name>A0A481QN62_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RecBCD enzyme subunit RecC</fullName>
            <ecNumber evidence="6">3.1.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Exonuclease V subunit RecC</fullName>
            <shortName evidence="6">ExoV subunit RecC</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Helicase/nuclease RecBCD subunit RecC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">recC</name>
        <name evidence="8" type="ORF">C3B55_00988</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit recognizes the wild-type Chi sequence, and when added to isolated RecB increases its ATP-dependent helicase processivity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.</text>
            <dbReference type="EC" id="3.1.11.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RecC family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.5" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00988"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009338">
        <property type="term" value="C:exodeoxyribonuclease V complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008854">
        <property type="term" value="F:exodeoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01486">
        <property type="entry name" value="RecC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006697">
        <property type="entry name" value="RecC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041500">
        <property type="entry name" value="RecC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17946">
        <property type="entry name" value="RecC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000980">
        <property type="entry name" value="RecC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01450">
        <property type="entry name" value="recC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecC_C" evidence="7">
        <location>
            <begin position="839"/>
            <end position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="1144" mass="131624" checksum="EF36948ADCAF21CE" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNB7</accession>
    <name>A0A481QNB7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase EpsE</fullName>
            <ecNumber evidence="3">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">epsE</name>
        <name evidence="3" type="ORF">C3B55_00849</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00849"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="19"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="387" mass="44503" checksum="3DEB527B931223B7" modified="2019-06-05" version="1">MSVEKSNILRNTDSIPLVSVVVPSYNYAKYISKAINSVINQSFSNFELLISDDCSLDNSWEVICNFNDPRIRTFQQEKNLGPVGNLIFLIQQARGKYIALLNSDDTWYPLKLSKQVMILTAQPKLGACFTWAKLVNETGKGISGPEVIWNDVFRQPNRTQAEWLKYFFLKGNSICHPSMLVRKEVFDKLGYYNPGLRQLPDFDMWIRLVKQFPIYIIQENLVTHMRSGNNISAVSLENSARNLTELVEIFSLFFESLADEVFTDGFSEYFKLKGVPVTQARLEAEKFFLLLESAFVQASGKAAALSWFIKRCTNPEFERVLRDEYMFSIFDFYQITGQAGFGHFFMAANQAPIPISTSVDEININTLKQGRIIYFLKCIVRHLRQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNC9</accession>
    <name>A0A481QNC9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00936</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00936"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="34" mass="4153" checksum="A2503AAB1FC292AC" modified="2019-06-05" version="1">MRVYAVIFRMLINNSIRNYKHNNKDSTFQCLKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QND8</accession>
    <name>A0A481QND8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rplU</name>
        <name evidence="6" type="ORF">C3B55_00984</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">This protein binds to 23S rRNA in the presence of protein L20.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Contacts protein L20.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00984"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01363">
        <property type="entry name" value="Ribosomal_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="L21-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="L21p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001787">
        <property type="entry name" value="Ribosomal_L21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018258">
        <property type="entry name" value="Ribosomal_L21_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="PTHR21349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="SSF141091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00061">
        <property type="entry name" value="L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01169">
        <property type="entry name" value="RIBOSOMAL_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="105" mass="11892" checksum="476B566BD5DA9573" modified="2019-06-05" version="1">MSYAVIVTGGKQYSVTQNEYLKIEKLEVATGSSITFDRVLLVTNGDDVRIGAPVVAGATVTAKVISKNRNDKISIIKFRRRKHYMKRMGHRQWYTEIKITDIQAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNF5</accession>
    <name>A0A481QNF5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="11">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">trpB</name>
        <name evidence="13" type="ORF">C3B55_01002</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 11">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01002"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="62"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX82300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="409" mass="44594" checksum="D397FA9BA414CC0F" modified="2019-06-05" version="1">MTQSQICLRNGPDAKGLFGAFGGCYVAETLMPLILNLTSEYEAAKIDPEFNEKLAYFQRDYVGRPSPLYFAQRLTEFCGGAKIYLKREELNHTGAHKINNCIGQILLARRMGKQRIIAETGAGMHGVATATVAARFAFDCVIYMGATDIERQQANVFRMKLLGAEVIQVSAGTSTLKDAMNEALRDWVTNVNNTYYLIGTVAGPHPYPTMVRDFQAVIGQEVRTQLQDQEGRLPDSLIACIGGGSNAMGLFHPFLDDASVEIIGVEAAGYGIETGKHAASLNGGVPGILHGNRTFLLQDDDGLIINTHSISAGLDYPGIGPEHAWLHNLGRVQYTSVTDNEALNAFHKCCNLEGIIPALESAHALAEAFKRAPNLPKDHLMVVNLSGRGDKDMQTVMQHMEIAPQEKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNK4</accession>
    <name>A0A481QNK4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9 11">Integration host factor subunit alpha</fullName>
            <shortName evidence="9">IHF-alpha</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">ihfA</name>
        <name evidence="9" type="synonym">himA</name>
        <name evidence="12" type="ORF">C3B55_00786</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9 11">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00786"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13835">
        <property type="entry name" value="IHF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00380">
        <property type="entry name" value="IHF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005684">
        <property type="entry name" value="IHF_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00987">
        <property type="entry name" value="himA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 9 11" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="100" mass="11643" checksum="BABEF26023118F2F" modified="2019-06-05" version="1">MKALTKAEIAERLCEELSLNRREAKELVELFFKEIRYALAENEQVKLSGFGNFDLRDKRQRPGRNPKTGEYTPITARRVVTFRPGQKLKARVEAYTKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNL3</accession>
    <name>A0A481QNL3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbonic anhydrase 1</fullName>
            <ecNumber evidence="2">4.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cynT</name>
        <name evidence="2" type="ORF">C3B55_01000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01000"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="PTHR11002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="SSF53056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="184" mass="20465" checksum="83877DF9FB4C753C" modified="2019-06-05" version="1">MFITCTDLRIVLELITHSSPGDLFVTRNVGNVVPPYGQMNGSVSTAIEYAVLTLDVQHIIVCGHSNCSAMRMVLYPDSLENMSTVRAWLRHADVAKSIVKDNFDFANESASMQALTEENVIAQLQHLRTHPSVALRMASGYLFIHGWIYNIETSDISAYDVDLATFRLLNDIDTIPCATPRVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNP3</accession>
    <name>A0A481QNP3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 14">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="7">IMP dehydrogenase</shortName>
            <shortName evidence="7">IMPD</shortName>
            <shortName evidence="7">IMPDH</shortName>
            <ecNumber evidence="7 14">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 16" type="primary">guaB</name>
        <name evidence="16" type="ORF">C3B55_00947</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="7">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 14">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 13">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="7 14"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00947"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
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        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3 7 14" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10 14" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="93"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="152"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7 9">
        <location>
            <begin position="337"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7 9">
        <location>
            <begin position="360"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7 9">
        <location>
            <begin position="384"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="7 8">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="247"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <begin position="297"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX82249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="489" mass="51783" checksum="65ED6CC63A929D7F" modified="2019-06-05" version="1">MLRISQEALTFDDILLVPGYSEALPNEVSLKTFLTSGIELNIPLVSAAMDTVTEARLAIAMAQEGGIGIIHKNMTIEQQAAEVRKVKRYEAGVVKDPITIDADATVRDLFDLTIMHNISSVPVLHAGDLVGIVTSRDVRFESRLAVTVRAVMTPKERLVTIKEGAAKSDVRKLLHRHRIERVLIVNDNFALKGMMTVNDIEKAKAYPLASKDDQGRLRVGAAVGTGKDIGDRVSALVANGVDVVVVDTAHGHSKGVVDSVRWVKQNFPNMQVIGGNIATGAAAKALVAAGADAVKVGIGPGSICTTRIVAGVGVPQISAIANVVFALEGTGIPLIADGGIRFSGDLSKAIAVGASSVMIGSMFAGTEESPGEIELFQGRSYKAYRGMGSLGAMFQAQGSSDRYFQDSLVGAEKLVPEGIEGRVPYKGSLSAIIHQLMGGLRSSMGYTGSADIEEMRTKPEFVRITGAGIVESHVHDVQLTKEAPNYRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNR1</accession>
    <name>A0A481QNR1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">FeS assembly protein IscX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">iscX</name>
        <name evidence="1" type="ORF">C3B55_00958</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007479">
        <property type="entry name" value="ISC_FeS_clus_asmbl_IscsX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036762">
        <property type="entry name" value="IscX-like_sf"/>
    </dbReference>
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        <property type="entry name" value="PTHR37532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04384">
        <property type="entry name" value="Fe-S_assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039003">
        <property type="entry name" value="IscX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140319">
        <property type="entry name" value="SSF140319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03412">
        <property type="entry name" value="iscX_yfhJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="66" mass="7790" checksum="998BF8DD6727300C" modified="2019-06-05" version="1">MNLKWVDVQEIAILLNARNPDLNPYSINFVVLRDMVMSLSEFEDEKNRSSEKILEAIQIAWQEEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNW8</accession>
    <name>A0A481QNW8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Oligopeptidase A</fullName>
            <ecNumber evidence="8">3.4.24.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">prlC</name>
        <name evidence="12" type="ORF">C3B55_01001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Hydrolysis of oligopeptides, with broad specificity. Gly or Ala commonly occur as P1 or P1' residues, but more distant residues are also important, as is shown by the fact that Z-Gly-Pro-Gly-|-Gly-Pro-Ala is cleaved, but not Z-(Gly)(5).</text>
            <dbReference type="EC" id="3.4.24.70"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.70" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01001"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06456">
        <property type="entry name" value="M3A_DCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034005">
        <property type="entry name" value="M3A_DCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024080">
        <property type="entry name" value="Neurolysin/TOP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045666">
        <property type="entry name" value="OpdA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PTHR11804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19310">
        <property type="entry name" value="TOP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TOP_N" evidence="11">
        <location>
            <begin position="29"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M3" evidence="10">
        <location>
            <begin position="225"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="683" mass="77210" checksum="AD8F1C0422BFAC78" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QP79</accession>
    <name>A0A481QP79_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">rpsP</name>
        <name evidence="4" type="ORF">C3B55_00942</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00942"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_S16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_S16_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="PTHR12919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="SSF54565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="83" mass="9250" checksum="D1513CF9E81C6006" modified="2019-06-05" version="1">MLAIRLALGGAKKRPFYYLTVTDSRNPRDGSHKEHVGFFNPIACGQEVRLFVNQERIAYWMSVGAQPTARVTKLLKDSVNAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKI0</accession>
    <name>A0A481QKI0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="8">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">tyrS</name>
        <name evidence="10" type="ORF">C3B55_00132</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-tyrosyl-tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00132"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02007">
        <property type="entry name" value="Tyr_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024108">
        <property type="entry name" value="Tyr-tRNA-ligase_bac_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="PTHR11766"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF1">
        <property type="entry name" value="PTHR11766:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="399" mass="44408" checksum="6DAC8A8294A2629A" modified="2019-06-05" version="1">MKSVEEQLALIKFGAAELLGEADLIKKLKSGQPLRIKAGFDPMAPDLHLGHTVLIDKLRQFQELGHQVIFLIGDFTGMIGDPSGKSATRPPLTREQVLNNAETYKTQAFNILDPATTEVVFNSVWMDQMKPADFISLASQYTVARMLERDDFDKRYSTNQSIAIHEFLYPLVQGYDSVALYADVELGGTDQKFNLLMGRELQRAYGQEAQCVLTMPLLVGLDGVKKMSQSLGNYVGIQEAPGVMYSKLVSMPDFLMWRYLELLSFRSMDEIKALRYKVEAGTNPRDLKITLAEEIIARYHGEEAAVSAHRAVGNRMRDGYLPDNVPEIELKATESMPIATVLNKAALVKNAAVARDLLGSGVVRIDGKIVDRTFLYELSATHVCQVGKKVFARITLKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKI2</accession>
    <name>A0A481QKI2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Murein DD-endopeptidase MepM</fullName>
            <ecNumber evidence="9">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mepM_1</name>
        <name evidence="9" type="ORF">C3B55_00131</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00131"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042834">
        <property type="term" value="F:peptidoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045834">
        <property type="entry name" value="Csd3_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007340">
        <property type="entry name" value="Opacity-associatedA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19425">
        <property type="entry name" value="Csd3_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04225">
        <property type="entry name" value="OapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="OapA" evidence="7">
        <location>
            <begin position="107"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Csd3_N2" evidence="8">
        <location>
            <begin position="198"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="6">
        <location>
            <begin position="331"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF19425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="470" mass="52293" checksum="8734CB2CF55F1F9D" modified="2019-06-05" version="1">MSIPQSNILIVYLKAYLLVTSSITACFSLVLLVCHFCDVEAKRISRSFDIENQFKKLIHNQDISSIQQAIHAPTKSLFAQMDSTPEDVQVITQEQSTPINQKNPLYRQITVTKGDTLSTLFEKIGLPAYTINKVLASNKQAKQFMQLKHGQKLEFELTSEGQLKNLHSTISDLESITLTKSAQGFAFNHIITEPVMRSAYIHGVINGSLSQSAACVGLSHSMTMNIASMFGYDIDFTQDIRQSDEFDIIYEQKIANGKIVGTGAILSARFTNRGKTYTAVHYTNKQGNSSYYTADGYNIHKTFIRTPVDFARISSRFSMGRKHPILNKIRVHKGVDYAAPSGTPIKAAGDGKVLLASRCGSYGNTVIIQHGDNYRTLYGHMQAFAKGLSIGSNVKQSQIIGYIGTTGLSTGPHLHYEFQINGVHVDPLSHKLLITSQISKTERMRFLQQSRPLIERMNQERSTLLASAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKJ2</accession>
    <name>A0A481QKJ2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine O-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cysE</name>
        <name evidence="6" type="ORF">C3B55_00143</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + L-serine = CoA + O-acetyl-L-serine</text>
            <dbReference type="Rhea" id="RHEA:24560"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.3.1.30"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00143"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="304" mass="33557" checksum="5BF6EC527083F123" modified="2019-06-05" version="1">MNEYCSHWHLQAIVSQLYKVRNQWRTRNGRVSREHGGRELPSREAVLRILELLRCALFPMRLGPVNLREKGEDFYVGHTLNVALNALLAQARLELRYAACHMTDNYNEVDSLAIHLIQDFALALPSLRSLLDTDILAAYQGDPAARSVDEVLLCYPGVLAVIHHRLAHHLYCAGLPLLARISAQIAHSVTGIDIHPGAQISPSFFIDHGTGVVIGETAIIGERVRIYQAVTLGAKRFPIDKHGQLKKNHLRHPIIEDDVVIYAGATILGRVTIGKGSIIGGNVWLTRSVPAGANITQAGLQHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKK5</accession>
    <name>A0A481QKK5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="2">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Phosphoriboisomerase A</fullName>
            <shortName evidence="2">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">rpiA</name>
        <name evidence="3" type="ORF">C3B55_00063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00063"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="PTHR11934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="232" mass="24843" checksum="AFBD53455F9ACFBD" modified="2019-06-05" version="1">MNYNQLKKVVAQAAINLILPKLNNKSIIGVGTGSTTNYFIDALAKYKSIFDGAVASSTTTAARLNKYGIPVYELNTVSDLEFYVDGADESDKHLNLIKGGGAALTREKIIAAVAKTFICIADASKLVPIIGAFPLPVEVIPMARSHVARELVKLGGDPVYREGILTDNGNIIIDVFNMQITNPVELEAKINAIVGVVTNGLFAARTADILLLGTPEGVETIKANQVENFQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKL6</accession>
    <name>A0A481QKL6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00166"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="76" mass="8579" checksum="16CBED45FC8730A1" modified="2019-06-05" version="1">MIASINIFTANYTLRQEYVYLSFSYFGKHLRFIVNLDLFTGNAFVKQSLSSYLSDQSTLTIANNNLANITLPAVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKM1</accession>
    <name>A0A481QKM1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Ribosomal RNA small subunit methyltransferase E</fullName>
            <ecNumber evidence="4 10">2.1.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rsmE</name>
        <name evidence="11" type="ORF">C3B55_00093</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) + N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42920"/>
            <dbReference type="Rhea" id="RHEA-COMP:10283"/>
            <dbReference type="Rhea" id="RHEA-COMP:10284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74502"/>
            <dbReference type="EC" id="2.1.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the RNA methyltransferase RsmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.193" evidence="4 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00093"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006700">
        <property type="entry name" value="rRNA_ssu_MeTrfase-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30027">
        <property type="entry name" value="PTHR30027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04452">
        <property type="entry name" value="Methyltrans_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015601">
        <property type="entry name" value="MTase_slr0722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00046">
        <property type="entry name" value="TIGR00046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8 10 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="239" mass="26550" checksum="BF80290A8BBA1DF1" modified="2019-06-05" version="1">MRFSRFFTNIPLNIGNHELPEALAYYISRVLRKRKGDTIRLFDGSGQEFLGILLEVGKQQVTVQLTKSFAGQTESPLYVYLGQGLSRSKRMDWAIQKATELGVNEITPIFSERCEIYLKGEHNSKRLLHWRQIAISACEQCGRSTVPVILPPLLLIDWLKQTEADLKLTLHPVAEPMTSYAKPASIAFLIGPAGGLTDTEIKTAQGAGFHTASLGPRILRTETTPVVALAVAQQLWGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKP6</accession>
    <name>A0A481QKP6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ADP-heptose--LPS heptosyltransferase 2</fullName>
            <ecNumber evidence="2">2.-.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rfaF</name>
        <name evidence="2" type="ORF">C3B55_00197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.-.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00197"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011910">
        <property type="entry name" value="RfaF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02195">
        <property type="entry name" value="heptsyl_trn_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="341" mass="38227" checksum="ECDCD6527A3312AB" modified="2019-06-05" version="1">MNILIVGPSWVGDMVIAQTLFQCLKQCHLDCQIDVLAPEWSRPILGRMLEVRQALCLPVNCRVIKLAMYRSFGKSMAGQYDQAILLPNSLKSALVPYFAGIPKRTGWRGEFRYVLLNDMRVLDRARYPLIMERFMALAYEPGAELPAPYPLTRLQINLVNRDVALAKFGLELDWPVLALCPGAEFGDSKRWPVEHYAKVAEMKIYDGWQVWLFGSKNDFSVGENIYKRISPSLRKKLINLSGYTTLAEAIDLLSCANLVVSNDSGLMHVAAALNLPLVAVYGSTSPNFTPPLANKVEIVRMGLDCSPCFKRVCPFGHYNCMRQLLPQLVSNALQRLESAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKP8</accession>
    <name>A0A481QKP8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3 7" type="primary">coaBC</name>
        <name evidence="7" type="ORF">C3B55_00032</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00032"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00353"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 4" id="KW-0288">FMN</keyword>
    <keyword evidence="3 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="5">
        <location>
            <begin position="7"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="DFP" evidence="6">
        <location>
            <begin position="186"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="191"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="402" mass="43071" checksum="9F1970040FDDE61C" modified="2019-06-05" version="1">MQRLYRKRIVLGVGGGIAAYKSAELVRRLLDQGAEVRVVMTRSGSEFITPLTMQALSGYPVHLDLLDPVAEAAMSHIDLAKWPDLVLIAPTTADLLARLAQGIADDLLTTLVLATDAIVAIAPAMNKTMWHNPVTQANTQLLQSRGFIVLGPASGSQACGDVGLGRMLEATDLVLCAAACFKHAALTGKNVLITAGPTQENIDPVRYITNHSSGIMGFALAEAAVEAGACVTLVTGPVRLPTPDNVYRINVISAQDMLTACIAAIPCDLFIASAAVADYRPEIIAPQKLKKDPASGDGLLLKMVRTTDILATIAKHVDRPFSVGFAAETEHLLDYAIRKLKDKNLDLIVVNDVNNPNIGFNSEENAISVIDRELRTTVFAQTNKSKLARQLILLIAQQLNQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKR8</accession>
    <name>A0A481QKR8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="1">4.2.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hemD</name>
        <name evidence="3" type="ORF">C3B55_00040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydroxymethylbilane = H2O + uroporphyrinogen III</text>
            <dbReference type="Rhea" id="RHEA:18965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="EC" id="4.2.1.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the uroporphyrinogen-III synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.75" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00040"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38042">
        <property type="entry name" value="PTHR38042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="SSF69618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEM4" evidence="2">
        <location>
            <begin position="18"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="249" mass="27370" checksum="ECC4996758E26841" modified="2019-06-05" version="1">MIDWRVLLTRPAEESAVLASILSKIGIFSSILPLLDIEALPVTAEQQSVFCNLDRYRAVIVVSKPAARLAERQLNKPWPQVAWFSVGAATAQVLANHGLNFSYPQTGNNSEALLELPALRKVIAQPGSRILIMRGEGGREFLAERLRTQGVNVDYLELYRRFLPDYDTGALMRRIKLERLNGLVVCSGQGFLHLQALAGHDWLKLARLPLFVPSSRVCAMARAAGAQKVVDCRGANVTALLMALRSAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKS9</accession>
    <name>A0A481QKS9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">ATP synthase subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP synthase F0 sector subunit a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">F-ATPase subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">atpB</name>
        <name evidence="13" type="ORF">C3B55_00005</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11 12">Cell membrane</location>
            <topology evidence="11 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00005"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01393">
        <property type="entry name" value="ATP_synth_a_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045082">
        <property type="entry name" value="ATP_syn_F0_a_bact/chloroplast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023011">
        <property type="entry name" value="ATP_synth_F0_asu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42823">
        <property type="entry name" value="PTHR42823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="SSF81336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01131">
        <property type="entry name" value="ATP_synt_6_or_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00449">
        <property type="entry name" value="ATPASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11 12" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 11 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 11 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="289" mass="32335" checksum="3B93C7590A28D08E" modified="2019-06-05" version="1">MTKVNASGYIQHHLQNLTFGQAPNGSWGFAHTIAEAKEMGFWAFHLDTLGLSVTLGLVFIMIFRMVAKKATSDQPGTLQNFIEILIEFVDNSVKDSFHGRSSVIAPLALTIFVWVFLMNAVDLIPVDWIPQLAMWITGDHHIPFRAVSTADPNTTMGMAMSVFVLIIFYSIKIKGISNFVGELTLHPFGSKNIFVQTMLIPANFLLEFVTLIAKPISLALRLFGNMYAGELIFILIAVMFNSDLLWLGGLGIILQLLWAVFHILIIVLQSFIFMMLTIVYLSMAHEDNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKT0</accession>
    <name>A0A481QKT0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription termination factor Rho</fullName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-dependent helicase Rho</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rho</name>
        <name evidence="11" type="ORF">C3B55_00050</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. The homohexamer assembles into an open ring structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the Rho family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00050"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008186">
        <property type="term" value="F:ATP-dependent activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04459">
        <property type="entry name" value="Rho_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01128">
        <property type="entry name" value="rho_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01884">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041703">
        <property type="entry name" value="Rho_factor_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011112">
        <property type="entry name" value="Rho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036269">
        <property type="entry name" value="Rho_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011113">
        <property type="entry name" value="Rho_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004665">
        <property type="entry name" value="Term_rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46425">
        <property type="entry name" value="PTHR46425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07498">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07497">
        <property type="entry name" value="Rho_RNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00959">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68912">
        <property type="entry name" value="SSF68912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00767">
        <property type="entry name" value="rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51856">
        <property type="entry name" value="RHO_RNA_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 8" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Rho RNA-BD" evidence="10">
        <location>
            <begin position="48"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA-binding 1" evidence="8">
        <location>
            <begin position="61"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA-binding 1" evidence="8">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA-binding 1" evidence="8">
        <location>
            <begin position="108"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA-binding 2" evidence="8">
        <location>
            <begin position="284"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="169"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="181"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="RNA-binding 2" evidence="8">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="419" mass="46958" checksum="DEA80C3457A13DFF" modified="2019-06-05" version="1">MNLTELKQKPIADLLQLAEKMGIENMARSRKQDIIFSMLKKHAKSGEEISGDGVLEILQDGFGFLRSADASYLAGPDDIYVSPSQIRRFNLRTGDTIVGKIRPPKEGERYFALLKVDTINFDRPENAKNKILFENLTPLFPTVRMTMEAGNGSTEDLTGRVIDLCAPIGKGQRGLIVAPPKAGKTIMLQNIAANIARNNQEVHLIVLLIDERPEEVTEMQRTVRGKVVASTFDEPPTRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGIDAHALEKPKRFFGAARNIEEGGSLTIIATALVETGSKMDEVIYEEFKGTGNMELPLDRRIAEKRVFPAININRSGTRREELLTADDELQRMWILRKLLHPMDEVAAIEFLVDKLKTTKTNDEFFLSMKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKT9</accession>
    <name>A0A481QKT9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00239"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="297" mass="32896" checksum="D5C7616889C686EF" modified="2019-06-05" version="1">MRALAEFIMHSRVQAALVVAGCVSLPLFYWLGAAAGCLILLKYGLKNSFGVLSLGLLSALIWWLQFSDPRVLLVLLGSSSLALVLRASESWVRTLLVSVALGLIYSVILSKVFCSQIEALSQEIIKILPMIIEDFYRQLLVGERARLALMITPILTGLIAALLQFVSVLSLILGRYWQAVLYNPGGFSREFYNIRLPVELVMLLLVCIVSGLYCGSQLVLLALICSVPLVFAGLALIHGLVAQKYLAKFWLVGLYVTFLLFMQLIYPLLVILAIFDGLIDFRSRLTLRDADNSSDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKV3</accession>
    <name>A0A481QKV3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Dihydrofolate reductase</fullName>
            <ecNumber evidence="3 7">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dhfrIII</name>
        <name evidence="10" type="ORF">C3B55_00072</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15009"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="3 7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00072"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="PTHR48069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000194">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="9">
        <location>
            <begin position="6"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="170" mass="19247" checksum="2E07E1DA401706D6" modified="2019-06-05" version="1">MKKTFSFSLIAAIGDNHVIGVNNTIPWYLPDDFKYFKATTLGKPIIMGRKTWDSIGRPLPGRLNIVVSRQTGLYLEGAEIFSSLDAAIKGAEVWALEHNVDEIMLIGGAQLYKQWLAQVDRLYLTRVALSPEGDTWFPEFNKQQWKLVLSLKNPAIDSKPAYSFEVWEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKX0</accession>
    <name>A0A481QKX0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6 8">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">rplC</name>
        <name evidence="9" type="ORF">C3B55_00277</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.</text>
    </comment>
    <comment type="PTM">
        <text evidence="6">Methylated by PrmB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00277"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_L3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_L3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="PTHR11229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="modified residue" description="N5-methylglutamine" evidence="6">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="211" mass="22696" checksum="43A4649AADE6A933" modified="2019-06-05" version="1">MTIGVVGRKCGMTRIFTKKGTSIPVSVIEIEQNRVTQFKTKLTDGYCAVQVTVGERRVSRITAAQAGHFVKANVAAGRTIVEFRLEDGQYQAGDLINVGIFAVGQLVDITGNSKGKGFQGTIKRWNFHGQDNTHGNSVSHRVPGSIGQCQTPGRVFKGKKMSGHMGAKRVTVQSLEVVRIDAERNLLLVKGAVPGANSGNLIVRPAVKFHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKX4</accession>
    <name>A0A481QKX4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inner membrane protein YohD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">yohD</name>
        <name evidence="2" type="ORF">C3B55_00045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00045"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="211" mass="24437" checksum="ECC7DF4F43D0F2B6" modified="2019-06-05" version="1">MLQQFLHDFGYFALFLGTFFEGETILVIAGFLAFRGYMDIKIVIVVTFFGSYAGDQLWYFLGRKHGRKLLSRKPRWQVMCDKALEHLCKHPDIWVLSFRFIYGLRTVMPIAIGLSGYPPTRYLTFNGIGAAIWATALGSAAYHFGSVLENMLNIVTKYELWILWILLVLGFSLQLRRRFKNMGITRKYYAKDKALRLAKLAQTKKSRTPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKX9</accession>
    <name>A0A481QKX9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00038"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="85" mass="9719" checksum="5E2F1C2E93B97EEC" modified="2019-06-05" version="1">MTLVYSNRVDINEGKTCKAGCRLSARRLNYKEVSTLIGWHLVVKCLIWNCLVLPINQYQLMSFISDCISVTFLDTVVLSLQLMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL02</accession>
    <name>A0A481QL02_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inner membrane transport protein YajR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">yajR</name>
        <name evidence="7" type="ORF">C3B55_00307</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00307"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="16"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="464" mass="49709" checksum="B093A003BB4C3367" modified="2019-06-05" version="1">MHDHCSERMSSSETRAASGLALVFAFRMLGMFMVLPVLATYGMDLAGATPALIGLAIGAYGLTQAFFQIPFGIISDRIGRRPVIYLGLILFALGSVLAAQSNTIWGVIAGRVLQGAGAISAAVIALLSDFTREQHRTKAMAIIGITIGLSFSAAMVIGPLLTRVSGLRGLFLTAGGMALLGIMIVAFIVPRSTSTLAHRESSLVRRALLPILKHPALLCLDLGICVLHAILMCSFVALPLALIEKAGLPKEQHWWVYLTALLISLFVMIPFINYSEKKRKIKRVFLSAIVTLMLIELFFWRFGDSLWTLVIGTIVFFTAFNLLEALLPSLVSKVSPAGSKGTAIGVYATSQFLGSALGGVMGGWISQHGGLSVVFLGSAGLAAFWLAFAVIMHEPPYVISIRLPLSPEAICEAGLVERLETVAGIVDAFVVAEEAAIYIKLDTELLDRATLEQLVNQSPVVRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL20</accession>
    <name>A0A481QL20_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Oligoribonuclease</fullName>
            <ecNumber evidence="3">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">orn</name>
        <name evidence="5" type="ORF">C3B55_00225</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">3'-to-5' exoribonuclease specific for small oligoribonucleotides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the oligoribonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00225"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06135">
        <property type="entry name" value="Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00045">
        <property type="entry name" value="Oligoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022894">
        <property type="entry name" value="Oligoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046">
        <property type="entry name" value="PTHR11046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="4">
        <location>
            <begin position="6"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="180" mass="20645" checksum="C116F7B063E96C97" modified="2019-06-05" version="1">MHNPKNLIWIDLEMTGLNPDTDVIIEMATIVTDSNLNTLAEGPVIAIHHSKAVLATMDEWNARIHGKSGLTQRVHDSRISMADAELKTIVFLEKWVPMGKSPICGNSVCQDRRFIYTHMKALENYFHYRNLDVSTLKELAARWAPEVKDSFHKNSTHLALDDIRQSIAELQHYRKHFIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL49</accession>
    <name>A0A481QL49_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00193"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="61" mass="7020" checksum="0AC58F04F3F3334D" modified="2019-06-05" version="1">MYRRPAQDDHQLVAVDEMACRAFMTSASPQENYQSVHQTLQVVAPWARMLFNKVNKVQPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL90</accession>
    <name>A0A481QL90_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Phosphoglucosamine mutase</fullName>
            <ecNumber evidence="6 8">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 13" type="primary">glmM</name>
        <name evidence="13" type="ORF">C3B55_00367</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="6">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="6 8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00367"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05802">
        <property type="entry name" value="GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_B">
        <property type="entry name" value="GlmM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006352">
        <property type="entry name" value="GlmM_bact"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01455">
        <property type="entry name" value="glmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="10">
        <location>
            <begin position="4"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="11">
        <location>
            <begin position="156"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="12">
        <location>
            <begin position="257"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="9">
        <location>
            <begin position="400"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="6">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="6">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="445" mass="47921" checksum="668E4AA00AD73ED8" modified="2019-06-05" version="1">MTKKYFGTDGIRGRVGIFPITAEFMLKLGWAAGMAFRSMGACRILVGKDTRISGYMLESALEAGLSAAGADVMLLGPMPTPAIAYLTRTLHAEAGIVISASHNPYDDNGIKFFSGDGTKLLDEIELIIEKLLDTPMTAVKSSQLGKVSRINDAPGRYIEFCKSSVPASINFTGLKIVIDCAHGATYKVAPSVFRELGADVTLLSAQPNGLNINHNCGSTHMEQLQTAVLAKHADLGIAFDGDGDRVLMVDHAGVVVDGDELLFIIARDLYECNKLQGGVVGTMMSNLGLELAMASMGIPFVRANVGDRYVIAELLERNWLVGGESSGHVVCFQHTTTGDAIIAALQVLLSLFRREESLAQACQALCKYPQVLLNVHFASTENPIEHSVIKNAHERITLAMAGHGRVLLRKSGTEPLVRVMVEGEDAAQVRLHAEELAKLVTEIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL97</accession>
    <name>A0A481QL97_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Chaperone protein ClpB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">clpB</name>
        <name evidence="13" type="ORF">C3B55_00426</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer; The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00426"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017730">
        <property type="entry name" value="Chaperonin_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03346">
        <property type="entry name" value="chaperone_ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Clp R" evidence="12">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="412"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="858" mass="95847" checksum="8407721E2CDD8E67" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLA0</accession>
    <name>A0A481QLA0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bifunctional ligase/repressor BirA</fullName>
            <ecNumber evidence="2">6.3.4.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">birA</name>
        <name evidence="2" type="ORF">C3B55_00258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.4.15" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00258"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin-[acetyl-CoA-carboxylase] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="6"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="267" mass="30039" checksum="4FA579421AC45CCB" modified="2019-06-05" version="1">MFLNVEEIIARAAPLAWPVYLYNSIGSTNTEAIRLISDTDYTAPFLVLAEQQTTGKGRRGRKWISPFAQNVYFSLVLQIDGGLQQLEGLSLVVGLAVMHSLRALSVHGAALKWPNDILVGHKKIAGILLELVGNPIDICHIVLGIGINANMQASMEIHQQWTSIQLETGFPIDRNYLIAKLWLQLQDYLTRHKTFGFIALQEEWERNHLWQGKAVSLIADVNQIDGIVLGVDRYGALRLNLDGVETIYRSGELSLRLRDDSRVRLWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLA1</accession>
    <name>A0A481QLA1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucose-6-phosphate isomerase</fullName>
            <shortName evidence="8">GPI</shortName>
            <ecNumber evidence="8">5.3.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphoglucose isomerase</fullName>
            <shortName evidence="8">PGI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphohexose isomerase</fullName>
            <shortName evidence="8">PHI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">pgi</name>
        <name evidence="10" type="ORF">C3B55_00379</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8 9">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8 9">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00379"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="PTHR11469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 8 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="554" mass="61530" checksum="03903C89744FCD1D" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-02-10" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLA3</accession>
    <name>A0A481QLA3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription antitermination protein NusB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Antitermination factor NusB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nusB</name>
        <name evidence="8" type="ORF">C3B55_00313</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the NusB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00313"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00073">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011605">
        <property type="entry name" value="NusB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11078">
        <property type="entry name" value="PTHR11078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01951">
        <property type="entry name" value="nusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusB" evidence="7">
        <location>
            <begin position="29"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="165" mass="19066" checksum="4A0E8093E1EB0194" modified="2019-06-05" version="1">MIYDESDRFNPRDPYLTASKLSKSDKRSAARQLATQALYQRHISGASLNEIEAQFCVDNDFTYADQNYFHDILYGVHTNLTKIDTALAPCLDLHIKRLDPVELCVMRLSTWELLYCIDVPYRVIINEGIELAKVYGSTDSHKFVNGVLNNMATRLRKAEFKERKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLB0</accession>
    <name>A0A481QLB0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Octanoyltransferase</fullName>
            <ecNumber evidence="6 7">2.3.1.181</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Lipoate-protein ligase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Lipoyl/octanoyl transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 12" type="primary">lipB</name>
        <name evidence="12" type="ORF">C3B55_00331</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6 7">Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)-octanoyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="EC" id="2.3.1.181"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6 7">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="6 7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00331"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16444">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00013">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016262">
        <property type="entry name" value="LPLase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="11">
        <location>
            <begin position="31"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6 9">
        <location>
            <begin position="70"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6 9">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6 9">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6 8">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="6 10">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR016262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="215" mass="23810" checksum="CBF76A5B17222313" modified="2019-06-05" version="1">MLQVLGFRELGQMIYTPVWHAMQRFTNERGTSAQDEVWLVEHQPVFTQGQSGKAEHLLLPGNIPVIQVDRGGQVTYHGPGQLVAYLLIDVRKLGLGVRDLVSRIEACLIELLANYGVSVVTKPDAPGVYVDDAKIASLGLRIRHGCSFHGLALNIDMDLRPFLRINPCGYAGLAMTQLRDHVTSIKFAEVSARLRAQLVKHLNYAEQITLTDGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLB3</accession>
    <name>A0A481QLB3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.2" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00212"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="313" mass="34289" checksum="B2D60F5EB086903F" modified="2019-06-05" version="1">MNNAEKWVLITGGAGFIGSHLVDALLTKGYAIRVLDNLSTGKRSNLPLGNPRLQLIEGSVASAELLIQSTVDTSAVVHLAAVASVQASINDPVNTHQSNFVGTLNVCEAMRKASVKRVVFASSAAVYGNKGSKGVAINEETTKAPLTPYASDKLSGEYYLDFYRRQHSLDPVIFRFFNIFGPRQDLVSPYSGVISIFSERVRHGMPIAIFGDGEQTRDFMYVEDLVNLLVQAIEAPEAPLGAINVGWNRATTLKQVLQALKEVVGQLPVITYGRVHSWDIRHSRANNQRLLENFRMPEPTPLKAGLERLINSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLB9</accession>
    <name>A0A481QLB9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00341</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00341"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014949">
        <property type="entry name" value="DUF1820"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08850">
        <property type="entry name" value="DUF1820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028538">
        <property type="entry name" value="DUF1820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="109" mass="12660" checksum="761E8F9047806F2D" modified="2019-06-05" version="1">MIKREVQIYKVIFLNQGQVFEIYAKQIYQSDLWGFLEVEGFIFGERTHLVVDPGEEKLKAQFEGVVRSFVPMHSIIRIDEVERLGTPKISEKCSMSNVMPFPPIPMPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLC3</accession>
    <name>A0A481QLC3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 7" type="primary">rplF</name>
        <name evidence="7" type="ORF">C3B55_00292</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 5">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00292"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_B">
        <property type="entry name" value="Ribosomal_L6_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019906">
        <property type="entry name" value="Ribosomal_L6_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002358">
        <property type="entry name" value="Ribosomal_L6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF14">
        <property type="entry name" value="PTHR11655:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00059">
        <property type="entry name" value="RIBOSOMALL6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03654">
        <property type="entry name" value="L6_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00525">
        <property type="entry name" value="RIBOSOMAL_L6_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L6" evidence="6">
        <location>
            <begin position="12"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L6" evidence="6">
        <location>
            <begin position="90"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="177" mass="19402" checksum="B37909B6BFEE74E2" modified="2019-06-05" version="1">MSRIAKNPVKLPASIQVKFIGQKLSVKGVKGTLELNIHPSVDVFEKTGGLFFAARNGDQQARAMVGTTRALVNNMVQGVSQGFERKLQLVGVGYKAQSKGTVLNLALGFSHQIDYELPEGVFAETPSQTDVVIKGIDKQLIGHVAAEIRKFRPPEPYKGKGVRYADEVIRRKDAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLC7</accession>
    <name>A0A481QLC7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sulfate adenylyltransferase subunit 1</fullName>
            <ecNumber evidence="13">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-sulfurylase large subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Sulfate adenylate transferase</fullName>
            <shortName evidence="13">SAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">cysN</name>
        <name evidence="15" type="ORF">C3B55_00415</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">APS kinase catalyzes the synthesis of activated sulfate.</text>
    </comment>
    <comment type="function">
        <text evidence="13">With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer composed of CysD, the smaller subunit, and CysN.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="13"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00415"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00204"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04166">
        <property type="entry name" value="CysN_ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03695">
        <property type="entry name" value="CysN_NodQ_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04095">
        <property type="entry name" value="CysN_NoDQ_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00062">
        <property type="entry name" value="Sulf_adenylyltr_sub1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041757">
        <property type="entry name" value="CysN_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044138">
        <property type="entry name" value="CysN_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044139">
        <property type="entry name" value="CysN_NoDQ_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011779">
        <property type="entry name" value="SO4_adenylTrfase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02034">
        <property type="entry name" value="CysN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 13 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tr-type G" evidence="14">
        <location>
            <begin position="22"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="110"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="165"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX81761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="632" mass="70481" checksum="DC1AB4704B262A5A" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLD7</accession>
    <name>A0A481QLD7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 9">Nicotinate phosphoribosyltransferase</fullName>
            <shortName evidence="8">NAPRTase</shortName>
            <ecNumber evidence="4 8 9">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pncB2</name>
        <name evidence="8" type="synonym">pncB</name>
        <name evidence="12" type="ORF">C3B55_00243</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O + nicotinate = ADP + diphosphate + nicotinate beta-D-ribonucleotide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8 9">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8 9">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="4 8 9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00243"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01401">
        <property type="entry name" value="PncB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00570">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006406">
        <property type="entry name" value="Nic_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="PTHR11098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01514">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 8 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NAPRTase_N" evidence="11">
        <location>
            <begin position="15"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="NAPRTase" evidence="10">
        <location>
            <begin position="172"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine; by autocatalysis" evidence="8">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="404" mass="46745" checksum="B84D943C5D76BB14" modified="2019-06-05" version="1">MSESVFTDRIVQNLLDTDFYKLTMMQAVLHNYPNVEVAWEFRCRNSEDLSQYLTEIRYQIELLTELNLDQDKLGFLEGISFIKPDFLRFLGLFRFNLRYVKTGIEKGELFIRLLGPWLHVILFEVPMLAIVSEVRNRYRYQTVLIEQAREQLYRKFYWLTANASSDELSELQVADFGTRRRFSYLVQEDVVKALKHDFPGHFVGTSNVHLAYKFNIRPLGTMAHEWIMAHQQLGPRLIDSQIAALDCWVREYRGLLGITLTDCITTDAFLNDFDLYFAKLFDGLRHDSGDPVQWAEKAINHYNKLGIEPMSKTLVFSNSLSLPKALEIFRTLRGRINVRFGIGTNLTCDIPGIQPMSIVLKMTACNGQPVAKISDETGKTHCNDQNFVAYLRHVFNVPDISSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLE0</accession>
    <name>A0A481QLE0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpmG</name>
        <name evidence="6" type="ORF">C3B55_00034</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00034"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00294">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="L33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001705">
        <property type="entry name" value="Ribosomal_L33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00471">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01023">
        <property type="entry name" value="rpmG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="51" mass="6073" checksum="422FCF1212A9752F" modified="2019-06-05" version="1">MRELIRMISSAGTGHFYTTDKNKRANPDKLEKKMFDPRVHRHVIYKEGKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLE5</accession>
    <name>A0A481QLE5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00254"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="66" mass="7520" checksum="04D541CF35697989" modified="2019-06-05" version="1">MLQLLSVVIIVGCYFCQFYRSYTIVSNKVPLAVAMKDLIGLNGLHCQFFVEVRLLEWRSGIPVILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLF8</accession>
    <name>A0A481QLF8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00398"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="38" mass="4308" checksum="23265AF13099DFAE" modified="2019-06-05" version="1">MKGSCSDFDGTENRIAAIQDDYLKVIYLCFILPLRAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLF9</accession>
    <name>A0A481QLF9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Intracellular sulfur oxidation protein DsrF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dsrF</name>
        <name evidence="2" type="ORF">C3B55_00461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DsrF/TusC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00461"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017462">
        <property type="entry name" value="Sulphur_relay_TusC/DsrF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38780">
        <property type="entry name" value="PTHR38780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03010">
        <property type="entry name" value="sulf_tusC_dsrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="115" mass="12471" checksum="0BD8C3A7F52F7AA2" modified="2019-06-05" version="1">MQSLLVISRQAPWSGLGALDALDIVLAGGVFDLPIGLLFMDDGVFQLAPDQNAAAVQQRDLCANLQVLNLFGIDDIFVCSHSLAERQLVSPTSVQVLSNNAIYQLIDQYNQIITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLG8</accession>
    <name>A0A481QLG8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1 2">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 4" type="primary">pth</name>
        <name evidence="4" type="ORF">C3B55_00401</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PTH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1 2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00401"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00083">
        <property type="entry name" value="Pept_tRNA_hydro_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PTHR17224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="SSF53178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01195">
        <property type="entry name" value="PEPT_TRNA_HYDROL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="194" mass="21178" checksum="4354C241108D74F8" modified="2019-06-05" version="1">MTTIKLIVGLGNPGSEYKKTRHNAGALFVERTSRTQSVNLVADCKYFGLTGRFIHQGQDVHLLIPTTYMNRSGQAVVALAGFFRIKPEEIIVAHDEMNLPPGTAKLKFSGGHGGHNGLRDIITQLGCQNNFYRLQLGIGHPGAASIVSNFVLSRAGYDEQKKLDVSIDFALSVLPKILAGKWNCAMQDLNNQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLH1</accession>
    <name>A0A481QLH1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Glutamate racemase</fullName>
            <ecNumber evidence="2 7">5.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">murI</name>
        <name evidence="8" type="ORF">C3B55_00410</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Provides the (R)-glutamate required for cell wall biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate = D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12813"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="EC" id="5.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aspartate/glutamate racemases family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.3" evidence="2 7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00410"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008881">
        <property type="term" value="F:glutamate racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00258">
        <property type="entry name" value="Glu_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018187">
        <property type="entry name" value="Asp/Glu_racemase_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004391">
        <property type="entry name" value="Glu_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF2">
        <property type="entry name" value="PTHR21198:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="SSF53681"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00067">
        <property type="entry name" value="glut_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00923">
        <property type="entry name" value="ASP_GLU_RACEMASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="25"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="57"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="89"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="200"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="279" mass="30652" checksum="CA5E3ECC2B54E94C" modified="2019-06-05" version="1">MSIVTPVSGYAGFHMFKNLPIGVFDSGIGGLSVLYEIQQLLPHESLLYLADCRYIPYGEKSSAFILKRSRLAAEFFRKQNAKVFVIACNTATIAAVSDLRQDYPKWPLVGMEPAIKPAAAATRSCVVGILATTSTLQSAKFTTLLNRFATDMRVITQPCPGLVELIETGDLNSATLLQMLHGYLRPLLSEGCDTIILGCTHYFFLKPLLAQILPRDIIIIDTGPAVALQLKHLLNEHDLLLASGNPKSVQFWTSGNVDHLRNILPILWKSPGVLNSFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLI2</accession>
    <name>A0A481QLI2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">Succinyl-diaminopimelate desuccinylase</fullName>
            <shortName evidence="15">SDAP desuccinylase</shortName>
            <ecNumber evidence="5 15">3.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 15">N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15 17" type="primary">dapE</name>
        <name evidence="17" type="ORF">C3B55_00495</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate</text>
            <dbReference type="Rhea" id="RHEA:22608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:58087"/>
            <dbReference type="EC" id="3.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="15">Binds 2 Zn(2+) or Co(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase M20A family. DapE subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.18" evidence="5 15"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00495"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009014">
        <property type="term" value="F:succinyl-diaminopimelate desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01690">
        <property type="entry name" value="DapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005941">
        <property type="entry name" value="DapE_proteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01246">
        <property type="entry name" value="dapE_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 15" id="KW-0170">Cobalt</keyword>
    <keyword evidence="11 15" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 15 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="M20_dimer" evidence="16">
        <location>
            <begin position="182"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX81831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="382" mass="41525" checksum="F5290C34E9BC51A9" modified="2019-06-05" version="1">MAHATFSLTIQLAIDLIRRPSITPIDANCQKLMMQRLGDVGFTLESMRIEAVDNFWATHGQNDGPVLCFAGHTDVVPTGPVQAWQYDPFNALIDENGMLCGRGAADMKGSLAAMLVASQRFVTDYPDHNGLVAFLITSDEEGPAQYGTKAVVERLAARKKRLDWCIIGEPSSATLVGDVVKNGRRGSLSATLTLHGVQGHVAYPHLAKNPIHMAVPALAELVAEHWDDGNTFFQPTSLQISNLHSSTCVTNVIPGHLTVMFNFRFSTESTMEGLQKRVAQILDKHSLDWHIEWVLSGLPFLTKPGVLLGAVSASIKAITGRETKISTSGGTSDGRFIATLGAQVIELGPVNATIHQVNERILASDLDVLTEIYYQTMIRLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLJ1</accession>
    <name>A0A481QLJ1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase C</fullName>
            <ecNumber evidence="1">2.1.1.172</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA m2G1207 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (guanine-N(2)-)-methyltransferase RsmC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 4" type="primary">rsmC</name>
        <name evidence="4" type="ORF">C3B55_00505</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the guanine in position 1207 of 16S rRNA in the 30S particle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(1207) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(2)-methylguanosine(1207) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42736"/>
            <dbReference type="Rhea" id="RHEA-COMP:10213"/>
            <dbReference type="Rhea" id="RHEA-COMP:10214"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74481"/>
            <dbReference type="EC" id="2.1.1.172"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RsmC family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.172" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00505"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052914">
        <property type="term" value="F:16S rRNA (guanine(1207)-N(2))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01862">
        <property type="entry name" value="16SrRNA_methyltr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013675">
        <property type="entry name" value="Mtase_sm_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023543">
        <property type="entry name" value="rRNA_ssu_MeTfrase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08468">
        <property type="entry name" value="MTS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS_N" evidence="3">
        <location>
            <begin position="5"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="MTS" evidence="2">
        <location>
            <begin position="162"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="332" mass="36169" checksum="6467651E90B3A115" modified="2019-06-05" version="1">MDQRSEVLLRQARLFQGHLLLVGLPADDLLGRLPNAYGWSWHAGDQAILDASFPERSQFGVNVPNWGFDAAVIFLPKSKDLTDYLISAVAARLDGAELFLVGEKQGGIKSAFKQMIHCGKPRKLDNARHCQLWQVTVTGAPPAVELASLIRVFNIPLTNGLLKVVSLPGVFSHGRLDHGTKLMLKHLDKLPSGHLLDFGCGAGVLGSAVKLLYPHNTVTMLDVDAFAAASSRLTIDANGLDAEVLVGDGIDTAPINLNAILSNPPFHIGVHTNYSATENLLRKSAQHIARGGELRLVANKFLKYQILIEKHFGMCEIKAINNSFCVYRAKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLJ2</accession>
    <name>A0A481QLJ2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH-quinone oxidoreductase subunit N</fullName>
            <ecNumber evidence="5">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NADH dehydrogenase I subunit N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NDH-1 subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">nuoN</name>
        <name evidence="8" type="ORF">C3B55_00435</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the complex I subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00435"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00445">
        <property type="entry name" value="NDH1_NuoN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010096">
        <property type="entry name" value="NADH-Q_OxRdtase_suN/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01770">
        <property type="entry name" value="NDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="7">
        <location>
            <begin position="147"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="508" mass="54694" checksum="A6309B61B32B6573" modified="2019-06-05" version="1">MAYSIGLALPFLNLLQSGKSAMELTIQHLSALAPLLITNLTIVVVMLTIAWNRNHLQTFMLSCAGLNLALLSIIQALKVAPLVVTKLMIIDNFALLYIALILIATIACIILAHAHIGEGSTGYPGNKEELYLLLLLAAAGGIVLVSSQHLAELLIGLELLSIPTYGLVTYAFFNKRSLEAGIKYMVLSTASSAFLLFGMALLYAEAGSLSFTGIGHAIAATKMPAPSAQLGLTIMLIGLSFKLSLVPFHLWTPDVYEGAPAPVAAFLATVSKVAVFAVMVRLFQISPAANTGVLSNVLTVIAIASILFGNLLALTQSNLKRLLSYSSIAHFGYLLIALVASKGLAVEAICVYLVTYVLNSLGAFGVITLMSAPFQDCDTDTLHTYRGLFWRRPYLTIVLTLIMLSLAGIPLTAGFIGKFYIVATGVEAHEWWLVTSLVLGNAISLFYYLRVMITLYMIGPSLRYVDTELHWEQKAGGVMLLAIAIFVFLLGVYPQPLLTIVRYTTLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLJ4</accession>
    <name>A0A481QLJ4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rplD</name>
        <name evidence="7" type="ORF">C3B55_00278</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="function">
        <text evidence="5">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00278"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_L4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4/L1e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="PTHR10746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="200" mass="22194" checksum="B2310F86448CF848" modified="2019-06-05" version="1">MQLNVNNSKAIEVSRLAFGGEFNETLVHQAIVAYMAGSHQYSKQQKTRSEVRGGGKRPWRQKGTGRARAGTIRSPIWRGGGTTFAARPQNCTQKLNKKMYRAAIRSILSELVRTDRLVVVQDFTVELPKTKDLLNKLHSMGLTDVMIVSEAIDQNLYLAARNLLRVGVHDVQGSDPASLIAYDKVLITVSAVKKFEELLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLK8</accession>
    <name>A0A481QLK8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase PmbA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">C3B55_00527</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00527"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD_M" evidence="3">
        <location>
            <begin position="126"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="2">
        <location>
            <begin position="240"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="448" mass="48160" checksum="1F85ECFE13BBB4AB" modified="2019-06-05" version="1">MSADQSVGPQALPALQEKVEYILAEAKRQGASACEVAVSLEQGLSTSVRQQAVETVEFNRNQGFSISLYIGQRKGSASTSTGGLKAMSETVAAALAIAKHTSEDESSGLANQNLMAKELQDFNLFHAWNITPEQAIEKALICEAAAFDADARIKNSDGTILSTHQGCRVYGNSHGFIGGYASTCYHMSCVMIAEANGQMQRDYWYDMNLQGELLADPVSIGRRAAQRAVNRLGAHAVPTCKVPVLFSAEIAGGLFSSFLEAISGSNLYRKSSFLEGTIDKILFPEWLTIDERPCLMRTMGSSAFDGDGLATYAKPFVEKGKLVSYVLDTYAGRKLGLPSTANAGGVHNLFVTHSDEDQMALLRRMGSGLLVTELMGSGLNMATGDYSRGAVGFWVENGEIQFAVQEVTIAGNMHDMFKQIIAVGNDLELRSNIRTGSVLIEQMTVAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLN4</accession>
    <name>A0A481QLN4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'(2'),5'-bisphosphate nucleotidase CysQ</fullName>
            <ecNumber evidence="3">3.1.3.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">3'-phosphoadenosine 5'-phosphate phosphatase</fullName>
            <shortName evidence="3">PAP phosphatase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">cysQ</name>
        <name evidence="4" type="ORF">C3B55_00151</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Converts adenosine-3',5'-bisphosphate (PAP) to AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol monophosphatase superfamily. CysQ family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00151"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02095">
        <property type="entry name" value="CysQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006240">
        <property type="entry name" value="CysQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01331">
        <property type="entry name" value="bisphos_cysQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="93"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="278" mass="30130" checksum="9D0B15EAFF9B70E1" modified="2019-06-05" version="1">MNKLFFSSRFIAPVIELARQAGEVILSYWRTDLEVTSKLDNSPVTAADLAAHHLILAGLTALNPSIPVLSEEDADIDCNIRSCWQRWWLVDPLDGTQEFISGSEEFTVNIALIEQGDVVFGVVLIPTSGCCYFGGAGLGAWRSNLNAMPQQIQVRQAPSAGEVFTVVVSRRHSTAEQERLLSGLSETLGELQLENIGSSLKFCLLAEGSADCYPCLAPTSQWDTAAAQGVLEGAGGEVLELNGEPFSYLARESLLNSFFLALPAKAAWREHLLSLARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLP3</accession>
    <name>A0A481QLP3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histidinol dehydrogenase</fullName>
            <shortName evidence="5">HDH</shortName>
            <ecNumber evidence="5">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 12" type="primary">hisD</name>
        <name evidence="12" type="ORF">C3B55_00512</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5 10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6 11">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00512"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="PTHR21256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="2 5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 10" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5 7">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5 7">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9 10">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 9 10">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9 10">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9 10">
        <location>
            <position position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="447" mass="48432" checksum="42DBB0DB07843D55" modified="2019-06-05" version="1">MVKPTSIIRLNADDSDFTHHLAHLLRRESVSDDLINQRVLNIIKAVRECGDKALIDFTSQFDKLDVKSMSDLIILHERLELALMRITAPQRDALMIAAARVRSYHEKQKQNSWSYTEADGTILGQKVTPLDSAGLYVPGGKASYPSSVLMNAIPAKVAGVAEVIMVVPTPHGEINELMLAAACIAGVDRVFTIGGAQAIAAMAYGTESVPKVDKVVGPGNIYVTTAKRHVFGQVGIDMIAGPSEILVVCDGQTNPAWIVMDLFSQAEHDEDAQAILISPDAEFLDKVTAIINELLPTMDRAAIIEKSINDRSALILVRDIEQAIEVVNLIAPEHLELSITNPQTWLPHIRHAGAIFMGRHTSEALGDYCAGPNHVLPTSGTARFSSPLSVYDFQKRSSIIFCSPQGASELGKTASVLARGESLSAHARSAEYRILDLLDSNSKGTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLQ0</accession>
    <name>A0A481QLQ0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">D-alanine--D-alanine ligase</fullName>
            <ecNumber evidence="6 14">6.3.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">D-Ala-D-Ala ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">D-alanylalanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ddlA</name>
        <name evidence="14" type="synonym">ddl</name>
        <name evidence="18" type="ORF">C3B55_00397</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 14">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the D-alanine--D-alanine ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.4" evidence="6 14"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00397"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00047">
        <property type="entry name" value="Dala_Dala_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000291">
        <property type="entry name" value="D-Ala_lig_Van_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005905">
        <property type="entry name" value="D_ala_D_ala"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011127">
        <property type="entry name" value="Dala_Dala_lig_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01820">
        <property type="entry name" value="Dala_Dala_lig_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039102">
        <property type="entry name" value="Ddl/VanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01205">
        <property type="entry name" value="D_ala_D_alaTIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00843">
        <property type="entry name" value="DALA_DALA_LIGASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 14" id="KW-0133">Cell shape</keyword>
    <keyword evidence="13 14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 14" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="135"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR039102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="QAX81745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="354" mass="38916" checksum="0497D2E991DFA381" modified="2019-06-05" version="1">MSKMRVGIIFGGRSAEHEVSLQSAYNIVNALDLSRFKPILIGIDKAGHWYFNDTFDFLINRSSNKLAVVFSKTNRQAKEACWQSLLKHIDIVFPIVHGTFGEDGCLQGLLRMAGLPFIGSDVLGSAVCMDKDISKRLLRDAGIAVTPFITVTSRNIASISFKKVVNKLGLPLFVKPANQGSSVGVSKVINQLEYYAAVKLAMGFDEKVLIESAVQNREIECAILGNEKPIASGCGEIVVSNGFYSYDNKYTNNHAAQMIVPANISVAVSEHIRYLSIKAFEVLGCAGLARVDCFLTSDNEVLINEINSLPGFTNISMYPKLWHAAGMSYSELVSRLIELALERHARCMELKIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLR7</accession>
    <name>A0A481QLR7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00542"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009386">
        <property type="entry name" value="DUF1043"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39579">
        <property type="entry name" value="PTHR39579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06295">
        <property type="entry name" value="DUF1043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="138" mass="15774" checksum="228610A7C0A6B04E" modified="2019-06-05" version="1">MEHSFLFWLLLILAMFAGVAIGFLIARLLPNAAPNRTKRQLDDIQERFDSYQNEVVTHFTCTATMVKKLIKGYQEVQDHLTDGAARLAIDDSTHQRLLATLDFDAQKTILDRLTPPRDYAPKVPNSLGMLDEYYGFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLV2</accession>
    <name>A0A481QLV2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00667"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="73" mass="7956" checksum="557B769878317B81" modified="2019-06-05" version="1">MSNLSVVTPDHILANILDMKLNFIAKLCLIVSTTIQVVKMLSDNCPHNLILSFVLLAMTSVKPITTTIVAKCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLV4</accession>
    <name>A0A481QLV4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein translocase subunit SecE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">secE</name>
        <name evidence="8" type="ORF">C3B55_00264</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00264"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005807">
        <property type="entry name" value="SecE_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038379">
        <property type="entry name" value="SecE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33910">
        <property type="entry name" value="PTHR33910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01650">
        <property type="entry name" value="SECETRNLCASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00964">
        <property type="entry name" value="secE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="116" mass="13020" checksum="1FAC837B0F2C0E53" modified="2019-06-05" version="1">MIPKAKSSRFDFAKWPSVIILTTVGIAGNQYYSVLPILYRVLALLTITILAVFIGMQTAKGKSFAVLVKEASTEIRKVVWPTRQETTQTTLIVVTVVLVMALLLWGLDYLLYWLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLW8</accession>
    <name>A0A481QLW8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3 beta-hydroxysteroid dehydrogenase/Delta 5--&gt;4-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00687"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="346" mass="38352" checksum="2A19B20E8D205B29" modified="2019-06-05" version="1">MLNDVNNHVVVKKPTILLTGGTGFVGCSVLRTLINLEYPVVNITRKNIIPTLHGVQNFLIGSLINEIDWKSFLSGVNVVIHAAGCAHVMYDIHRNQLQTFRKVNVDVTLKLARQAATAGIKRFIFISSIKVNGEETSARSAFTSFDIPMPLDDYGRSKHEAEQALLELAGRISMEVVIIRPVLVYGPGVKANFECMLSVVNKKLPLPFRAINNRRSLVFIDNLVDLICVCINHPAAKNRVFLVSDDDDLSIGQILEKLAVALHTSPYLFTVPKFLLNTAALLTGQKKILQRLCGSLQVDMTDTRERLSWTPPVSVDEAFAVTARYFQEQQAGKVNSCINSLVKMPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLW9</accession>
    <name>A0A481QLW9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="7">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">lysU</name>
        <name evidence="7" type="synonym">lysS</name>
        <name evidence="10" type="ORF">C3B55_00499</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 8">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7 8">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00499"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="179"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="500" mass="57338" checksum="DC94FECCE2335F7D" modified="2019-06-05" version="1">MSDQQLDSQDIQKEENSLIALRKEKLAAVRAKGNVFPNNFRRNNYCNTLHNQYADKTKEELADAAIQVKVAGRIMLNRGSFIVIQDMTGRIQIYVNRKTLPEETLISVKKWDIGDIIAAEGTIARSRKGDLYVKITSVSLLTKSLRPLPDKHHGLTDTEQRYRQRYVDLIVNEDARQTFRVRSKVIAYIRNFLMQRDFLEVETPMLHTIPSGAAAKPFKTHHNALNMEMSLRIAPELYLKRLVVGGFEKVFEINRNFRNEGISPRHNPEFTMLEFYQAFTDYEDNMDLTEELFRELAQLVLGSTDVPYGDKVFHFGEPFVRLSVFDSILKYNPDLTADDLTDINKARAIAKKVCTSLFGFESLGKLQVIIFEKLVEHKLEQPHFITQYPFEVSPLARRNNDNPNLTNRFELFIGGREIANAYSELNDAEDQAERLMAQVIEKDAGNDEAMHYDADFIRALEYGMPPTAGEGIGIDRLVMLLTNSPSIRDVILFPHMRPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLX1</accession>
    <name>A0A481QLX1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA gyrase inhibitor YacG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">yacG</name>
        <name evidence="2" type="ORF">C3B55_00429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibits all the catalytic activities of DNA gyrase by preventing its interaction with DNA. Acts by binding directly to the C-terminal domain of GyrB, which probably disrupts DNA binding by the gyrase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with GyrB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA gyrase inhibitor YacG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00429"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008657">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00649">
        <property type="entry name" value="DNA_gyrase_inhibitor_YacG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005584">
        <property type="entry name" value="DNA_gyrase_inhibitor_YacG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36150">
        <property type="entry name" value="PTHR36150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03884">
        <property type="entry name" value="YacG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="66" mass="7643" checksum="DB2C07DDC80E976B" modified="2019-06-05" version="1">MNQLLTINCPTCGIFVKWTTTNHNQPFCSDRCKLIDLGAWASEKYKIQVKSDIKDEIFPEDLPLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLY1</accession>
    <name>A0A481QLY1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="15">ATP-PRT</shortName>
            <shortName evidence="15">ATP-PRTase</shortName>
            <ecNumber evidence="6 15">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 17" type="primary">hisG</name>
        <name evidence="17" type="ORF">C3B55_00513</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 15">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Heteromultimer composed of HisG and HisZ subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">Lacks the C-terminal regulatory region which is replaced by HisZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the ATP phosphoribosyltransferase family. Short subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="6 15"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00513"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13595">
        <property type="entry name" value="PBP2_HisGs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01018">
        <property type="entry name" value="HisG_Short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024893">
        <property type="entry name" value="ATP_PRibTrfase_HisG_short"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 15 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 15" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="11 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HisG" evidence="16">
        <location>
            <begin position="51"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX81847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="205" mass="22550" checksum="9AF2831575717F3C" modified="2019-06-05" version="1">MLTIALSKGRIFDDALPLLAEANIVPIENQNKSRKLIIPTTQDDVRLLIVRATDVPTYVEHGAADLGIAGKDVLMEYGSLNLYEILDLRIAMCKLMTAGRIGHTKPKGRLRVATKFVKVAKRYYVEHGSQVDIMKLYGSMELAPLLGLADKIIDVVDTGNTLLANGLEPQDLVANISSYLIANKASMKMQYARIQALISTLRKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-04-07" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM00</accession>
    <name>A0A481QM00_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LPS-assembly lipoprotein LptE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lptE</name>
        <name evidence="3" type="ORF">C3B55_00334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Together with LptD, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane. Required for the proper assembly of LptD. Binds LPS and may serve as the LPS recognition site at the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the lipopolysaccharide transport and assembly complex. Interacts with LptD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LptE lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00334"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01186">
        <property type="entry name" value="LPS_assembly_LptE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007485">
        <property type="entry name" value="LPS_assembly_LptE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38098">
        <property type="entry name" value="PTHR38098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="191" mass="21508" checksum="1DAFD9451462949A" modified="2019-06-05" version="1">MIKRNLMVIGLTVLLDACGFQLRSSTAINALNIKKLDVSASNAYGDTVTQLRQTLVNNGVHVVTGAIYKLNLIDEKETQRNLSYASVGRTPDIELSSVIIYELQDQDHLTLINDKITLKKVVSYNGNNLIGLDSIITQVRKEIRHELVQRIIMRLQQITPEKIAMLQQKVNNKTKASTLKNTQKYEDNTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM01</accession>
    <name>A0A481QM01_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00742"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="70" mass="8108" checksum="F949474AB849FC31" modified="2019-06-05" version="1">MSYNIKNKKNKLMLLILIAAKEHLILNVRCHMLTSIRKMSVMVNNLDLNTSAIIILVKVFFYTINVAKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM14</accession>
    <name>A0A481QM14_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-specific adenosine deaminase</fullName>
            <ecNumber evidence="3">3.5.4.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">tadA</name>
        <name evidence="5" type="ORF">C3B55_00554</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine(34) in tRNA + H(+) + H2O = inosine(34) in tRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:43168"/>
            <dbReference type="Rhea" id="RHEA-COMP:10373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10374"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.33" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00554"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00972">
        <property type="entry name" value="tRNA_aden_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028883">
        <property type="entry name" value="tRNA_aden_deaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14437">
        <property type="entry name" value="MafB19-deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="164" mass="17842" checksum="6B3D735D1B3484A7" modified="2019-06-05" version="1">MSQIEIFDHSRDQYFMRDALALAAQGAARGEVPVGAVLVQNGAIIGRGFNCPISSNDPSAHAEIVAIRIAATVISNYRLVDSTLYTTLEPCSMCAGLIVHSRITRLVYGALEPKAGIVQSQGRFFTQGFLNHRVLFKGGVLAEECRTVLGDFFRARRDGKLISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM16</accession>
    <name>A0A481QM16_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Outer membrane protein assembly factor BamE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">bamE</name>
        <name evidence="7" type="ORF">C3B55_00355</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BamE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00355"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00925">
        <property type="entry name" value="OM_assembly_BamE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026592">
        <property type="entry name" value="BamE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037873">
        <property type="entry name" value="BamE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007450">
        <property type="entry name" value="Lipoprotein_SmpA/OmlA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37482">
        <property type="entry name" value="PTHR37482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04355">
        <property type="entry name" value="SmpA_OmlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SmpA_OmlA" evidence="6">
        <location>
            <begin position="35"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="175" mass="19444" checksum="19325F32A80FA6BF" modified="2019-06-05" version="1">MQNIHILLTSVTIIVVLALTSCSFPKAYKNDIQQGNVITQDMINQLRPGMTRRQVQFIMGTPLLTNTFDANCWDYLYSMQSNGSGHQQKRVSLIFNVNDQLVSLSGDFMPDISHDKALLGKENITNMTANVRKTEKAQPEVSVKSHSLLDQIQKDIDGAKTVPVPAPQNLDTSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM34</accession>
    <name>A0A481QM34_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 7" type="primary">rpsO</name>
        <name evidence="7" type="ORF">C3B55_00377</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="3 6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00377"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_S15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_S15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="PTHR23321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="89" mass="10365" checksum="18B367F6457C150D" modified="2019-06-05" version="1">MVLDVQEKAQIIADYRQAVNDTGSPEVQVSLLTYNINKLQGHFRVNCKDHHSRRGLICMVNQRRKLLDYLKGKDLSRYQVLIGRLGLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM39</accession>
    <name>A0A481QM39_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vitamin B12 transport ATP-binding protein BacA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">bacA</name>
        <name evidence="10" type="ORF">C3B55_00544</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00544"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="39"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="371"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="404"/>
            <end position="411"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="575" mass="65871" checksum="E450B54F71909558" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM56</accession>
    <name>A0A481QM56_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Valine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Valyl-tRNA synthetase</fullName>
            <shortName evidence="8">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 13" type="primary">valS</name>
        <name evidence="13" type="ORF">C3B55_00565</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The C-terminal coiled-coil domain is crucial for aminoacylation activity.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00565"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="10">
        <location>
            <begin position="14"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="11">
        <location>
            <begin position="669"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="Val_tRNA-synt_C" evidence="12">
        <location>
            <begin position="885"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="921"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="551"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="554"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="948" mass="108306" checksum="A08464E15CBF7C6D" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM59</accession>
    <name>A0A481QM59_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Lon protease</fullName>
            <ecNumber evidence="5 6">3.4.21.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent protease La</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 13" type="primary">lon</name>
        <name evidence="13" type="ORF">C3B55_00603</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6 9">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="5">By heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6 9">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="5 6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00603"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="lon_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
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        <property type="entry name" value="Lon_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="12">
        <location>
            <begin position="7"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="11">
        <location>
            <begin position="587"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="779"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="780"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7 9">
        <location>
            <position position="674"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7 9">
        <location>
            <position position="717"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <begin position="352"/>
            <end position="359"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="798" mass="89171" checksum="223C692936A4B8E4" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM71</accession>
    <name>A0A481QM71_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH-quinone oxidoreductase subunit M</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">NADH dehydrogenase I subunit M</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NDH-1 subunit M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nuoM</name>
        <name evidence="9" type="ORF">C3B55_00436</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00436"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010227">
        <property type="entry name" value="NADH_Q_OxRdtase_chainM/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507">
        <property type="entry name" value="PTHR43507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01972">
        <property type="entry name" value="NDH_I_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="8">
        <location>
            <begin position="134"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="510" mass="56977" checksum="48A74A441C2F3DEC" modified="2019-06-05" version="1">MILPWLILIPFIGGQLCWISAHFRATLPRWIALLTMSVELSLSLWLWVHSDYSFIPIPGADPAFVFEFKQIWIQRFGINMHLAIDGLSLLMILLTGLLGILSVLCAWKEIQRYIGFFYFNLMWILGSVVGVFLALDLFMFFFFWEMTLVPMYFLIALWGHDSSDGKKTRIYAATKFFIFTQTSGLIMLVAILGLVLVNFNNTGIITFNYVDLLKTKISLTTEYILMLGFFIAFAVKLPAVPFHSWMPDTHAQAPIAGSVDLAGILLKTAAYGLLRFALPLFPNASAKFAPIAMTMGLVGIFYGAFLAFAQTDIKRLIAFSSISHMGFVLIGIYSNNQIALQGAIIQMLAHGISTAALFILTGQLYERLHTFDMREMGGLWARITYLPAINLFFCAASLGLPGTGNFIGEFLILMGAFVRTPWISAIATSGLVLNAIYLLIMIHRIYFGYAKSDILLQGMDARELIMTLSLALLILYIGIYPQPFLDISATTIYGIQHRLSIAFSQLASVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM75</accession>
    <name>A0A481QM75_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">UDP-3-O-acyl-N-acetylglucosamine deacetylase</fullName>
            <shortName evidence="12">UDP-3-O-acyl-GlcNAc deacetylase</shortName>
            <ecNumber evidence="4 12">3.5.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">lpxC</name>
        <name evidence="13" type="ORF">C3B55_00570</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine + H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate</text>
            <dbReference type="Rhea" id="RHEA:67816"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:137740"/>
            <dbReference type="ChEBI" id="CHEBI:173225"/>
            <dbReference type="EC" id="3.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 2/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the LpxC family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.108" evidence="4 12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00570"/>
    <dbReference type="UniPathway" id="UPA00359">
        <property type="reaction ID" value="UER00478"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008759">
        <property type="term" value="F:UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103117">
        <property type="term" value="F:UDP-3-O-acyl-N-acetylglucosamine deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00388">
        <property type="entry name" value="LpxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004463">
        <property type="entry name" value="UDP-acyl_GlcNac_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011334">
        <property type="entry name" value="UDP-acyl_GlcNac_deAcase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015870">
        <property type="entry name" value="UDP-acyl_N-AcGlcN_deAcase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33694">
        <property type="entry name" value="PTHR33694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03331">
        <property type="entry name" value="LpxC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00325">
        <property type="entry name" value="lpxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="303" mass="33170" checksum="C315D3D46E5AA183" modified="2019-06-05" version="1">MIKQRTIKNSIHATGVGLHSGEKVYLTLRPAPIDSGIVFVRADLDPVVQISARAENVGETTMSTTLVNGNVKVDTVEHLLSAMAGLGIDNAYVELSASEVPIMDGSAGPFVFLIQSAGLEEQDAEKKFIRILREVKVEDGDKRATFVPFQGFKVSFEVDFDHPAFRAHTQSATVNFSSTSFVKEISRARTFGFMSDIEYLRKHNLALGGSVDNAIVVDADGVLNEDGLRYEDEFVKHKILDAIGDLYLLGNSLIGEFKGFKSGHALNNQLLRKLISQTGAWEIVTFKDANATPISYMRPAVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM85</accession>
    <name>A0A481QM85_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutamine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutaminyl-tRNA synthetase</fullName>
            <shortName evidence="9">GlnRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">glnS</name>
        <name evidence="13" type="ORF">C3B55_00596</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00596"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00126">
        <property type="entry name" value="Gln_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022861">
        <property type="entry name" value="Gln_tRNA_ligase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020061">
        <property type="entry name" value="Glu_tRNA_lig_a-bdl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1c" evidence="11">
        <location>
            <begin position="39"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1c_C" evidence="12">
        <location>
            <begin position="351"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="279"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="46"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="52"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="272"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="575" mass="66561" checksum="E3736EEAE8D1C37E" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM86</accession>
    <name>A0A481QM86_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00642</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00642"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="43" mass="4962" checksum="EB6EB2E73BE6E143" modified="2019-06-05" version="1">MIAEDNTSVSYLVCITMQKILIFQAYRVVYIARSSKQNQHLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMB0</accession>
    <name>A0A481QMB0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00746"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="62" mass="7377" checksum="AB2D094586688A15" modified="2019-06-05" version="1">MAIEKHRVFCVRLMPHDKFTGLLLEDIINLLKRQADNSQMHTYHNEDKASLCYDKFLSDILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-04-07" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMC0</accession>
    <name>A0A481QMC0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="5">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroF</name>
        <name evidence="7" type="ORF">C3B55_00683</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00683"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00084"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DHAP_synth_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PTHR21225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP_synth_1" evidence="6">
        <location>
            <begin position="45"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="358" mass="39283" checksum="B63A120B071706EB" modified="2019-06-05" version="1">MTDLPINDLNIAFSETLITPDQLKREIPLSHAALQTVTNGRKVIRDILDGIDHRLFVVIGPCSIHDLHATHKYAEHLKVLAIEMSDTLYLVMRVYFEKPRTTIGWKGLINDPYLDDSFKIQDGLYISRKLLLELAEMGLPTATEALDPISPQYLQDLISWSGIGARTTESQTHREMASGLSSAVGFKNGTDGGLTVAINALQSVSSPHRFIGINQDGGVSIITTTGNSYGHVVLRGGNGKPNYDSIGVALCEQALHKAKIKPNIMVDCSHANSNKDPALQPLVMENVTNQILEGNQSIIGLMVESHLNWGCQTIPKDLAGLQYGVSITDACIDWSATQTTLRSMHTKLKKILPNRKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMC1</accession>
    <name>A0A481QMC1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00396</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00396"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="74" mass="8137" checksum="AFE13136CE0B42CC" modified="2019-06-05" version="1">MDSSRESVLLALVSYKARNLKSLFTASATIAGFIWIKDYMASLLGKIYSMPPCVSSMAQRFNGKKIDLTSSDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMC6</accession>
    <name>A0A481QMC6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)</fullName>
            <ecNumber evidence="3">2.5.1.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Ditrans,polycis-undecaprenylcistransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="3">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="3">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ispU</name>
        <name evidence="3" type="synonym">uppS</name>
        <name evidence="4" type="ORF">C3B55_00767</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with (2E,6E)-farnesyl diphosphate (E,E-FPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E)-undecaprenyl diphosphate (di-trans,octa-cis-UPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = di-trans,octa-cis-undecaprenyl diphosphate + 8 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.31" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00767"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008834">
        <property type="term" value="F:di-trans,poly-cis-decaprenylcistransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="3" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="190"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="246" mass="27846" checksum="A7E09A514E2FCC13" modified="2019-06-05" version="1">MTELYVVPRHVAIIMDGNNRWAKKRFMPVVSGHKAGFDAVRAVIEVCAKAKIEVLTLFAFSSENWQRPVAEVNALMDLFFKALRREAKHLQENNISLRIIGDRSRFHSKLQTAILEAESITAGSNCFVLQIAANYGGQWDITQAAQRLAREAHAGHLRPEDITSELLQSCLATCDLPLPDLCIRTGGEHRISNFLLWQLAYTELYFSDLFWPDFKHDAMHNALIEFASRQRRFGKTSEQIDAGAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMD4</accession>
    <name>A0A481QMD4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable lipid II flippase MurJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">murJ</name>
        <name evidence="10" type="ORF">C3B55_00418</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the MurJ/MviN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00418"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015648">
        <property type="term" value="F:lipid-linked peptidoglycan transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13123">
        <property type="entry name" value="MATE_MurJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02078">
        <property type="entry name" value="MurJ_MviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004268">
        <property type="entry name" value="MurJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03023">
        <property type="entry name" value="MurJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002869">
        <property type="entry name" value="MviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01806">
        <property type="entry name" value="VIRFACTRMVIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01695">
        <property type="entry name" value="murJ_mviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 8 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8 9" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="521" mass="57612" checksum="BEBCCBC868751828" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMD9</accession>
    <name>A0A481QMD9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein-arginine rhamnosyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">EF-P arginine rhamnosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">C3B55_00681</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Protein-arginine rhamnosyltransferase that catalyzes the transfer of a single rhamnose to elongation factor P (EF-P) on 'Lys-32', a modification required for EF-P-dependent rescue of polyproline stalled ribosomes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dTDP-beta-L-rhamnose + L-arginyl-[protein] = dTDP + H(+) + N(omega)-(alpha-L-rhamnosyl)-L-arginyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66692"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:17096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57510"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:167445"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:66693"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyltransferase 104 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00681"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106361">
        <property type="term" value="F:protein-arginine rhamnosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016633">
        <property type="entry name" value="EarP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10093">
        <property type="entry name" value="EarP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015557">
        <property type="entry name" value="UCP015557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03837">
        <property type="entry name" value="efp_adjacent_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="377" mass="43899" checksum="4BD044D65CC0C2D8" modified="2019-06-05" version="1">MKACCDIFCRVIDNYGDIGIAWRLAKQLAVEHSYEVRLWVDNAHAFKRMRQEINVQLDQQWQEGVQVCHWRTDWPQTESADVVIAAFSCQLPLNYMEAMAARERTPLWINLDYLSAEDWVTGCNRLPSVKFKSVQKYFFFPGFRLGTGGLLREVGLLAQRQAFQQEVAVQRQFLQSVGVIPIADARLISLFAYENANLASWLDILAMDRHVTHLLVPYDCIISDVQHWLEVTSLAVGDMHQRNALTVQVLPFMRQEQYDHLLWCCDFNVVRGEDSFVRAQWAGRPLLWHIYHQDKNIHLDKLNAFLELYTTTLSQAAKTAVVALWQAWNTDGDMMQTWKMLLEHWQEVSLHSQKWCLEQSLQADLATSLVKFYESWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMH2</accession>
    <name>A0A481QMH2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alpha-ketoglutarate permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">kgtP</name>
        <name evidence="7" type="ORF">C3B55_00737</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00737"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004736">
        <property type="entry name" value="MHS_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00883">
        <property type="entry name" value="2A0106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="18"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="430" mass="47369" checksum="DBB1311BC320C00B" modified="2019-06-05" version="1">MITTGYYTGKERSKRIFAIIGASSGNLVEWFDFYVYAFCAMYFAPAFFPSDNPTVQLVNTAGIFAAGFLMRPIGGWLFGRVADKRGRKTSMMISVLMMCAGSLIIAVLPTYSDISVWAPILLLIARLFQGLSIGGEYGTTATYMSEVALKGQRGFFASFQYVTLIGGQLLAVLVVVILQQVLSEDELRTWGWRIPFLIGAIAAVISLLLRRTLKETISKEVRKDKDAGSIAALFRNHKAAFITVLGYTAGGSLIFYTFTTYMQKYLVNTAGMHAKTSSYIMTCALFLYMCMQPVFGMLADKIGRRNSMLWFASLGTLLTAPILLTLKTMHDPFLTFLLITLALAIVSFYTSISGLVKAEMFPPHVRALGVGLAYAIANAVFGGSAEVVALSLKSIGMENTFYWYVTAMMAVAFLFSLRLPKESAYLHHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-06-17" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMH9</accession>
    <name>A0A481QMH9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00724</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00724"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="78" mass="9323" checksum="2890B88614254E73" modified="2019-06-05" version="1">MNPDLNHVWIRANFALYHKPWRIEDINFSRSLYYINMVKIKEQREDKLSCSKLCGSKLSREKIETGTERYQVYPAMPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMI0</accession>
    <name>A0A481QMI0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Septum site-determining protein MinD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cell division inhibitor MinD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">minD_2</name>
        <name evidence="11" type="ORF">C3B55_00825</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">ATPase required for the correct placement of the division site. Cell division inhibitors MinC and MinD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with MinC and FtsZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParA family. MinD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00825"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010223">
        <property type="entry name" value="MinD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01968">
        <property type="entry name" value="minD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="10">
        <location>
            <begin position="3"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="270" mass="29299" checksum="D1BAFC54E0438238" modified="2019-06-05" version="1">MAKILVVTSGKGGVGKTTTSAAIGTGLALRDHKTVIVDFDVGLRNLDLIMGCERRVVYDLVNVVNSEASLQQALIKDKRLENLYVLAASQTRNKDSLTKEGVGEVLDKLKETFEYIVCDSPAGIEAGAHLAMYFADEAIIVTNPEVSSVRDSDRMLGLLASKSKRAQKGEAPIKEHLLLTRYNPERVSNGEMLGVADVNEILAVTMLGVIPESQAVLKASNQGIPVILDDQSDAGQAYSDAVDRLLGKTVKYRFLDVRKKGFFERIFGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMJ5</accession>
    <name>A0A481QMJ5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chaperonin GroEL</fullName>
            <ecNumber evidence="6">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Chaperonin-60</fullName>
            <shortName evidence="6">Cpn60</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">groL</name>
        <name evidence="6" type="synonym">groEL</name>
        <name evidence="10" type="ORF">C3B55_00928</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00928"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="525"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="87"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="479"/>
            <end position="481"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="549" mass="57229" checksum="F87C9226CB3F1407" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QML0</accession>
    <name>A0A481QML0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00779</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00779"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="59" mass="6677" checksum="936F2355C932F25C" modified="2019-06-05" version="1">MLGYGQSIEKTSTATDFVCQYHGAFSGIVKDIRGFAYFEHHGLMPISNTVNYQQFTVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QML1</accession>
    <name>A0A481QML1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UDP-2,3-diacylglucosamine hydrolase</fullName>
            <ecNumber evidence="9">3.6.1.54</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UDP-2,3-diacylglucosamine diphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">lpxH</name>
        <name evidence="11" type="ORF">C3B55_00597</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Hydrolyzes the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP by catalyzing the attack of water at the alpha-P atom. Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>H2O + UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine = 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl 1-phosphate + 2 H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:25213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:57957"/>
            <dbReference type="ChEBI" id="CHEBI:78847"/>
            <dbReference type="EC" id="3.6.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Binds 2 Mn(2+) ions per subunit in a binuclear metal center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 4/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the LpxH family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.54" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00597"/>
    <dbReference type="UniPathway" id="UPA00359">
        <property type="reaction ID" value="UER00480"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019897">
        <property type="term" value="C:extrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008758">
        <property type="term" value="F:UDP-2,3-diacylglucosamine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00575">
        <property type="entry name" value="LpxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043461">
        <property type="entry name" value="LpxH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010138">
        <property type="entry name" value="UDP-diacylglucosamine_Hdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34990">
        <property type="entry name" value="PTHR34990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34990:SF1">
        <property type="entry name" value="PTHR34990:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01854">
        <property type="entry name" value="lipid_A_lpxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="10">
        <location>
            <begin position="2"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="249" mass="28469" checksum="1A89F6773653D31F" modified="2019-06-05" version="1">MILLISDLHLAEERPDITLAFLDLLYSRAHRIKVLYILGDLFEVWINDDGMTTFQHLICAVLRKLSNSGALVFIMHGNRDFLIGKTFCKAAGATLLQDPTVVQFYGEPVLLMHGDSLCTRDIAYMKMRCILRNPITLYILRHLPLCARHKLASKLRNASRAKTKANNIVDVTPEEVPLVMQHFGVRTMVHGHTHRPAIHELQIGDQVAWRIVLGSWDQHGWALQVDEHSFTLSSFNFVRPLLRQTSYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMM7</accession>
    <name>A0A481QMM7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uridylate kinase</fullName>
            <shortName evidence="6">UK</shortName>
            <ecNumber evidence="6">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Uridine monophosphate kinase</fullName>
            <shortName evidence="6">UMP kinase</shortName>
            <shortName evidence="6">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">pyrH</name>
        <name evidence="8" type="ORF">C3B55_00769</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00769"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02075">
        <property type="entry name" value="pyrH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="7">
        <location>
            <begin position="13"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="18"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="141"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="245" mass="26454" checksum="3FBF992B578113F8" modified="2019-06-05" version="1">MAQQGCGRQARYKRILLKISGEALMGSEEFGIDPKVLDRMALEVGQLVGIGVQVGLVIGGGNLFRGAALSAAGMDRVTGDHMGMLATVMNALAMRDALERANISVIVMSAISMLGVTDHYDRRKAMRHLNSKEVVIFAAGTGNPFFTTDSAACLRAIEIDSDIMLKATKVDGVYTADPFKDQHAKKFDHLTYDEVLSLKLSVMDLTAICLCRDHEMPLRVFNMNKPGALLNIVHGGAEGTLIEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMR0</accession>
    <name>A0A481QMR0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="7">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">trpA</name>
        <name evidence="9" type="ORF">C3B55_01003</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01003"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="PTHR43406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6 7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="269" mass="28862" checksum="D67A06C984A2FA7C" modified="2019-06-05" version="1">MSRLQICFAQLKKQNQAAFITFMTAGDPSYNTSLAIFKGLPAAGADIIELGMPFTDPMADGPIIQLANIRALEAKQNLTKTLQMVKEFRKDNSNTPLILMGYFNPIHKYGVPQFIANAKAVGVDGLIIVDMPPEHNGELCDPAQAAGIDFIRITTPTTNDARLKTVLNDSSGFVYYISVAGVTGTGTATLKHIEGAVTRLRQHTNLPISVGFGIRTPKQAADIARLADGVVVGSALIDRIATAKNDQQAIDGVLSLCLALSDSIHNTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMS4</accession>
    <name>A0A481QMS4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase</fullName>
            <ecNumber evidence="7">6.3.2.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Meso-A2pm-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Meso-diaminopimelate-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UDP-MurNAc-tripeptide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UDP-N-acetylmuramyl-tripeptide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 12" type="primary">murE</name>
        <name evidence="12" type="ORF">C3B55_00581</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:23676"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
            <dbReference type="ChEBI" id="CHEBI:83905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MurCDEF family. MurE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.13" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00581"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008765">
        <property type="term" value="F:UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00208">
        <property type="entry name" value="MurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005761">
        <property type="entry name" value="UDP-N-AcMur-Glu-dNH2Pim_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01085">
        <property type="entry name" value="murE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 7 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7 8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7 8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="11">
        <location>
            <begin position="106"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="10">
        <location>
            <begin position="329"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-MurNAc-L-Ala-D-Glu binding" evidence="7">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Meso-diaminopimelate binding" evidence="7">
        <location>
            <begin position="402"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Meso-diaminopimelate recognition motif" evidence="7">
        <location>
            <begin position="402"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="108"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>meso-2,6-diaminoheptanedioate</name>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>meso-2,6-diaminoheptanedioate</name>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>meso-2,6-diaminoheptanedioate</name>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="7">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="487" mass="52698" checksum="530BAA6F0D8D9B09" modified="2019-06-05" version="1">MSFSQNKVFSYAQHDLLIRDLSLDSRNVRTGDLFLAVPGSHLDGRAYITNALERGAVAVVYELEGATVLPITDVPMIPIKGLAAQLSDIARQFYGDPSRQLNLVGVTGTNGKTSVTHLIAQALELLGQHCGIVGTLGAGFCGELQSGSHTTPNQIAVQATLMHLQKAGAETVAMEVSSHGLDQGRVAGLAFDVAVITNLSRDHLDYHGTMEAYAATKGRLFTWNDLKCRVVNLDDTFGRKLAIEYSNARLITYSLKDSSSYLYCCEAQFSDKGVLATLVTPQGKYYLCSTLIGRFNLSNMLASIGALLGLEYSLDEILHVLPKLKGPIGRMHCLGGGFRPLVVIDYAHTPDAIEKVLIALRPHAKGKLLCLFGCGGDRDRGKRPLMAKIVERLADSVLVTDDNPRSEDSGQIFDNIRVGFKDVCKVSFINDRSAAIAQLIASASVNDVVLLAGKGHETYQEINGKRYAFSDLVEADHALTTYEVAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMU0</accession>
    <name>A0A481QMU0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00923"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd16170">
        <property type="entry name" value="MvaT_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035616">
        <property type="entry name" value="MvaT_DBD"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="124" mass="14212" checksum="762333AEDABC9D87" modified="2019-06-05" version="1">MSLITEYRATEEAIKELQARLKNLSQDNNLQTELEFEGKLRTLMGEYSKSMRDIIALLDPELTVKAPRSAVKITSTKLARKVKQYKNPYNGEVIQTKGGNHKTLKEWKAKWGSNVVEDWSTLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMW2</accession>
    <name>A0A481QMW2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00838"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="43" mass="5204" checksum="924527FE4EA5A8DD" modified="2019-06-05" version="1">MYNTRVGNMQYTSIKNDMYRFAMSINLLVCLFRDIICLEVYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMW6</accession>
    <name>A0A481QMW6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00834"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="61" mass="6767" checksum="F3E9C2FC232998FE" modified="2019-06-05" version="1">MISANTGVLMSDLALADEPLTVFVSDSAYIYDGSIVRHRANTYLSLLPLTLYSYENKDAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMY5</accession>
    <name>A0A481QMY5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ</fullName>
            <ecNumber evidence="4">2.1.1.200</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA Cm32/Um32 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">trmJ</name>
        <name evidence="6" type="ORF">C3B55_00966</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-adenosyl-L-methionine + uridine(32) in tRNA = 2'-O-methyluridine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42936"/>
            <dbReference type="Rhea" id="RHEA-COMP:10107"/>
            <dbReference type="Rhea" id="RHEA-COMP:10290"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.200"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>cytidine(32) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42932"/>
            <dbReference type="Rhea" id="RHEA-COMP:10288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10289"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.200"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.200" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00966"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="PTHR42786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="5">
        <location>
            <begin position="5"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="256" mass="28425" checksum="50B8ED201EECD582" modified="2019-06-05" version="1">MLQNICVVLVNVSHPGNIGSVARAMKNMGLTRLVLVEPRAFPHNEANIRASGANDILEKAKIVATLENALVSCNLVFGTSARHRRISWPLLGLRQCGTKVVEEAASGTQIALVFGREDSGLTNKELQQCHYHVHIPSNPKFSSLNLGAAVQVVSCEVRIAWLIAQDQSSKLEKNEVASTKNSELATVDELERFYKHLEQTLVAIKFLKPEKPRYLMARLRCLYGRSSVSRTEINILRGILTETQKVVDSASRKRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN04</accession>
    <name>A0A481QN04_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">4-diphosphocytidyl-2C-methyl-D-erythritol synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">MEP cytidylyltransferase</fullName>
            <shortName evidence="7">MCT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">ispD</name>
        <name evidence="8" type="ORF">C3B55_00750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>2-C-methyl-D-erythritol 4-phosphate + CTP + H(+) = 4-CDP-2-C-methyl-D-erythritol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="EC" id="2.7.7.60"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.60" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00750"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00093"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050518">
        <property type="term" value="F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00108">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001228">
        <property type="entry name" value="IspD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018294">
        <property type="entry name" value="ISPD_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00453">
        <property type="entry name" value="ispD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01295">
        <property type="entry name" value="ISPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5 7 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="7">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="7">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="235" mass="26050" checksum="6745E161209D030A" modified="2019-06-05" version="1">MNKPLPDFWAVIPAAGFGIRMAADRPKQYLQLGERTILEHSLECFLDHPGLKGLVISLAVDDPYWQNLPCANDLRILRTAGGVERSGSVLNALLQLNALGVSDDNWVLVHDAVRPNLSRDDLNKLLAQLANDPVGGFLAVPARDTLKRVDKYGRVIETLDRSLIWQAYTPQMFRLGALCRALTNCLVSGVLISDEASAIEWSGQAQRLIEGRSDNIKVTRPEDLDWLRLCLANRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN15</accession>
    <name>A0A481QN15_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ</fullName>
            <ecNumber evidence="9">4.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase</fullName>
            <shortName evidence="9">(3R)-hydroxymyristoyl-ACP dehydrase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Beta-hydroxyacyl-ACP dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">fabZ</name>
        <name evidence="10" type="ORF">C3B55_00760</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the thioester dehydratase family. FabZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.59" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00760"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008659">
        <property type="term" value="F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008693">
        <property type="term" value="F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047451">
        <property type="term" value="F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004317">
        <property type="term" value="F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00406">
        <property type="entry name" value="FabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010084">
        <property type="entry name" value="FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01750">
        <property type="entry name" value="fabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="9">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="146" mass="16585" checksum="F3E7AC0BDBBACEE8" modified="2019-06-05" version="1">MMGINEIREYLPHRYPFLLVDRVVDLNVAEKRICAYKNVSINEPFFNGHFPAHPIMPGVLIIEAMAQAAGILGFKMLDLKPADGMLYYFVGSDKLRFRNPVIPGDQLILEARLISCKRQIWKFECQASVTSKPVCSAEIICAERKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN16</accession>
    <name>A0A481QN16_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Membrane-bound lytic murein transglycosylase F</fullName>
            <ecNumber evidence="7">4.2.2.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Murein lyase F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">mltF</name>
        <name evidence="9" type="ORF">C3B55_00945</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Exolytic cleavage of the (1-&gt;4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.</text>
            <dbReference type="EC" id="4.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell outer membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="7">Attached to the inner leaflet of the outer membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The N-terminal domain does not have lytic activity and probably modulates enzymatic activity. The C-terminal domain is the catalytic active domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transglycosylase Slt family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the C-terminal section; belongs to the transglycosylase Slt family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the N-terminal section; belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.n1" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00945"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016837">
        <property type="term" value="F:carbon-oxygen lyase activity, acting on polysaccharides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008933">
        <property type="term" value="F:lytic transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000270">
        <property type="term" value="P:peptidoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02016">
        <property type="entry name" value="MltF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023703">
        <property type="entry name" value="MltF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000189">
        <property type="entry name" value="Transglyc_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00922">
        <property type="entry name" value="TRANSGLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBPb" evidence="8">
        <location>
            <begin position="20"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="LT domain" evidence="7">
        <location>
            <begin position="246"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="462" mass="52159" checksum="FA15141269247339" modified="2019-06-05" version="1">MLGACVNKHSTLEQIKENGILRAITRNSPATYFQDHNGNTGFEYELVKLFADDIGVKLEIKTANNLDNLFSQLGKPNGPVIAAAGLVSSEQRAQQVLFSYPYLEVTPQIIYRHGQPRPTNLVDLAGKTIMALKGSTHAEQLAKLKKQNPAIEYEESDVAEVVDLLNMVDNGQIDLTLLDSNEVAMNQVYFPNIRVAFDLGNASNQSWAVVPGDDNSLLNEINKYLAKMEKNGTLKRLKDRYYGHVDVLGCVGAYTFAQHLQQRLPKYEKHFRAYAKDKNIDWRLLAAIGYQESLWQPTVTSKTGVRGLMMLTKNTAQAMGVSNRLDAKQSIMGGAKYLTKIKNKLDSDIAEPDRTWFALAAYNVGIGHLEDARMLAKKTGLNANKWLDVRQMLPRLSQKQWYSQTRYGYARGGEPVHFVANIQRYYDILTWVTKPHPDKREIIKGNLRILDIHKDMLQKDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN17</accession>
    <name>A0A481QN17_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal RNA subunit accumulation protein YceD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">23S rRNA accumulation protein YceD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">C3B55_00881</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in synthesis, processing and/or stability of 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DUF177 domain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00881"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003772">
        <property type="entry name" value="YceD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039255">
        <property type="entry name" value="YceD_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38099">
        <property type="entry name" value="PTHR38099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02620">
        <property type="entry name" value="YceD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="180" mass="20642" checksum="8A9AEA17D7449852" modified="2019-06-05" version="1">MFTPMLNNAFPIQVNPYKLADSNISLQGEMLLACFDRLCSQSFNTLNMVQIKFIFKRDERKSVVINSIINTEGQMVCQRCLELFTMPIHSKCNYVVVKEGADTQSLLKDYEVLELSADHLNLHELIEDELLLALPIVPVHNAEECQQPTWLNAEPKLSDNEIIRYSPFSILAQFKHNLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN30</accession>
    <name>A0A481QN30_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Elongation factor Ts</fullName>
            <shortName evidence="5">EF-Ts</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">tsf</name>
        <name evidence="7" type="ORF">C3B55_00770</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the EF-Ts family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00770"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="PTHR11741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="SSF54713"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00116">
        <property type="entry name" value="tsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01126">
        <property type="entry name" value="EF_TS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF_TS" evidence="6">
        <location>
            <begin position="71"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in Mg(2+) ion dislocation from EF-Tu" evidence="5">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="276" mass="29780" checksum="8DBEF89CE09DD5D0" modified="2019-06-05" version="1">MAEINTALVKKLRERTGEGMMDCKKALLKAGGEIEKAIDDMRASGAIKAAKKAGNIAIEGAIALKEDGKSAVLLEVNSQTDFLAIQDDFKAFVLASIEQAFADKLITTELLIKAQEAARIVLVGKVGENVNIRRLVRIEGDVVGGYLHGNTIGAVVALKGGDVKLAKDIAMHVAATHPEFLLPSEVSNAVIQREQAVFLRLNADKIAGKPENIVDNMINGRIRKFLAEVSLVEQAFIKNHAVKVGELVKKAGAEIVSFTYYKVGEDIEKPGDNFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN34</accession>
    <name>A0A481QN34_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Histidine transport system permease protein HisQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hisQ</name>
        <name evidence="7" type="ORF">C3B55_00898</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the binding-protein-dependent transport system permease family. HisMQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00898"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071705">
        <property type="term" value="P:nitrogen compound transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="13"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="229" mass="24979" checksum="FAD017BAA166B4BD" modified="2019-06-05" version="1">MLKGYEFVILDGVWLTLQLAVSSMALAVVFGLIGVILCLSPVRWLAWLGALYSTVIRGIPDLVLILLIFYGGQDLLNLVAPMLGYHAFIDLNPLAAGIGTLGFIFGAYLTETFRGAYIAIPKGQVEAGIAYGMSSFQVFFLVMVPQMIRLAIPGFTNNWLVLTKATALISVVGLQDMMFKAKQAADATREPFTFFLAVAVMYLLITSASLLVFRYLEKRYSIGVSAADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN46</accession>
    <name>A0A481QN46_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">YciI-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">C3B55_00909</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00909"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="YCII" evidence="3">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="87" mass="9233" checksum="65FF1B48761F7840" modified="2019-06-05" version="1">MLCSITATDVAGAPEKRKLVYLTYIEHLDALEAKGSLVIAGLNTVADSNDQNLAGFTGGLIIVELTSMAAAQALGIGRPMYHRKRLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN74</accession>
    <name>A0A481QN74_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 16">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="6 16">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">hom</name>
        <name evidence="19" type="ORF">C3B55_00938</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-homoserine + NAD(+) = H(+) + L-aspartate 4-semialdehyde + NADH</text>
            <dbReference type="Rhea" id="RHEA:15757"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="6 16"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00938"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016204">
        <property type="entry name" value="HDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000098">
        <property type="entry name" value="Homoser_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 16" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11 15 16" id="KW-0521">NADP</keyword>
    <keyword evidence="12 16 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="18">
        <location>
            <begin position="353"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="QAX82240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="434" mass="46189" checksum="4E004D3C4E1AFE99" modified="2019-06-05" version="1">MKPVKVGICGLGTVGSGTFNVLQRNAGEISRRAGRGIKVAQIATRTSKPQFQTTGIVMTNDVFALAANDEIDIVIELVGGYTVARDLVLLAIENGKHVVTANKALIAVHGNEIFAKACEHGVIVAFEAAVAGGIPVIQAIREGLSANRINWVAGIINGTGNFILTEMREKGRTFEDVLAEAQTLGYAEADPTFDVEGIDAAHKLTILASIAFGIPLQFDKVYTEGITKLTTADVSYAEALGYRIKHLGVARSTASGIELRVHPTLIPADRLIANVNGVMNAVMVNGDAAGSTLFYGAGAGMEPTASSVIADLVDVVRAMTSDPENRVPHLAFQPDSLSAHPILPIEACESAYYLRIQAKDHPGVLAQVARILSERGINIESIMQKEVEAQDGLVPMILLTHRVLEQHINNAITALEALHGVVGSVVRIRVEYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN79</accession>
    <name>A0A481QN79_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00817"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="40" mass="4421" checksum="8C89612556CFB9F7" modified="2019-06-05" version="1">MSDNKNHVAAHIIAAMNSTTQPLPHFNSTQTMAIFLHTND</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-04-07" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN83</accession>
    <name>A0A481QN83_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome maturation factor RimM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 5" type="primary">rimM</name>
        <name evidence="5" type="ORF">C3B55_00941</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Binds ribosomal protein S19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The PRC barrel domain binds ribosomal protein S19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RimM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00941"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="16S_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RimM" evidence="3">
        <location>
            <begin position="14"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="PRC" evidence="4">
        <location>
            <begin position="102"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="178" mass="20022" checksum="56DDCB929112DF47" modified="2019-06-05" version="1">MNATLAVVDNLIIIGKIYSSYGVRGEVKVYSFINPTKDSLQYKTWTLKRGLSAKQVELAGGRWSHKFLIAKLKGLENREDARLLAGYDVCVHRNLFPALTDGEYYWYQLIGLKVIDQLGQLLGKIDYVLETGANDVIVVKSCTGSLDNRERLLPYTEQCILAVNLAVGEMKVDWDADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNF4</accession>
    <name>A0A481QNF4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RecBCD enzyme subunit RecD</fullName>
            <ecNumber evidence="10">3.1.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Exonuclease V subunit RecD</fullName>
            <shortName evidence="10">ExoV subunit RecD</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Helicase/nuclease RecBCD subunit RecD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">recD</name>
        <name evidence="12" type="ORF">C3B55_00986</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.</text>
            <dbReference type="EC" id="3.1.11.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the RecD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.5" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00986"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009338">
        <property type="term" value="C:exodeoxyribonuclease V complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008854">
        <property type="term" value="F:exodeoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01487">
        <property type="entry name" value="RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006344">
        <property type="entry name" value="RecD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041851">
        <property type="entry name" value="RecD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027785">
        <property type="entry name" value="UvrD-like_helicase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13538">
        <property type="entry name" value="UvrD_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01447">
        <property type="entry name" value="recD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD_C_2" evidence="11">
        <location>
            <begin position="611"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="215"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="691" mass="77415" checksum="FAB6A3E1D5500EB9" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNI5</accession>
    <name>A0A481QNI5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Superoxide dismutase</fullName>
            <ecNumber evidence="2 6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sodB</name>
        <name evidence="9" type="ORF">C3B55_00904</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 H(+) + 2 superoxide = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00904"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="SSF46609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="SSF54719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sod_Fe_N" evidence="7">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Sod_Fe_C" evidence="8">
        <location>
            <begin position="91"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="198" mass="22682" checksum="AAF5BC2DAAD8ECC9" modified="2019-06-05" version="1">MAFELSPLPYEYDALQPHISKETLEYHHDKHHNTYVVNLNNLVPGTEFEDKTLDEIVMSSSGDIFNNAAQVWNHTFYWNCLAPNNDDYAIGALASAIDVTFGSFDKFKEEFIKTSVRIFGSGWSWLVKKTDGSLAIASTIGAGNPLTSYDIPLLTCDVWEHAYYIDYRNVRLKYIEAFWSLVNWKFVTEQFEGKTVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNN1</accession>
    <name>A0A481QNN1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">16S rRNA (cytosine(967)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.176</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">16S rRNA m5C967 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">rRNA (cytosine-C(5)-)-methyltransferase RsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rsmB</name>
        <name evidence="13" type="ORF">C3B55_01010</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(967) in 16S rRNA + S-adenosyl-L-methionine = 5-methylcytidine(967) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42748"/>
            <dbReference type="Rhea" id="RHEA-COMP:10219"/>
            <dbReference type="Rhea" id="RHEA-COMP:10220"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.176"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.176" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01010"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004573">
        <property type="entry name" value="rRNA_ssu_MeTfrase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00563">
        <property type="entry name" value="rsmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 11 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="12">
        <location>
            <begin position="161"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="11">
        <location>
            <begin position="251"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX82308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="438" mass="48751" checksum="FA9FE31B4168F274" modified="2019-06-05" version="1">MNPRLAAAKALATVLNGQSLLNSSLLIQLNKVEDHDRSFTKNLAFGAARWQPRLSVLSAILLRKPFKAVDADIEALLLVGLYQLIYTRVPAYAAISETVDCADKLHKPWAKALLNAVLRRAQRESVALFAERKHDPVVRTAHPRWLQKSLKKFWPEQWETICAANNEHPPMTLRVNRFHYNRDTYLKLLTDACINAMPCVYSIDGIVLESATDVSTLPGFTEGWVSVQDEAAQLAANLLDLAPHQRVLDVCCAPGGKTCHILEVEKNLASMVAIDVKAKRLVRVRENLVRLGLSAKLIAADGRDTTTWWDGKLFQRILLDAPCSSTGVIRRHPDIKLTRKPNDIATLSILQGELLDAVWPILEVGGILLYTTCSILPTENTEVIKDFLSRTQNAHELGLATTAGIKQRYGRQLLTQKGGHDGFYYAKLVKSSIVCRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNV4</accession>
    <name>A0A481QNV4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00864</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00864"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="97" mass="11021" checksum="6B607DC565E76B71" modified="2019-06-05" version="1">MQVSCTVQPCFSLKTDAFCGRLHYIIVSHVSIMVQILVPAQIRANYISALSLLRIRMLRLKILLVSCINKHALPIKANYCGYMLGLYAQQDVLLFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QPA1</accession>
    <name>A0A481QPA1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ancillary SecYEG translocon subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">C3B55_00953</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the YfgM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00953"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018704">
        <property type="entry name" value="TPR_21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026039">
        <property type="entry name" value="YfgM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38035">
        <property type="entry name" value="PTHR38035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09976">
        <property type="entry name" value="TPR_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006170">
        <property type="entry name" value="YfgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="TPR_21" evidence="5">
        <location>
            <begin position="17"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="214" mass="23662" checksum="3F6A04BA31B042D8" modified="2019-06-05" version="1">MGTAMLSADDDQLSEFKDWWKRNCKLLVIGGLFVLVVVFGRQAWYQYQANQSRNASTTYQQLLEIMLILDDKLDLAQVVSQVDKLKHEFRNSTYAQYGSLFVAKVAVDTGKLDDAVIELKTISEKSILGEIARHRLAKVLAAQNKTNEALKLLSSHSDKTFTATCEELKGDLLVQIGDLDGAYAAYQAAQAELSDKSAVGVLQIKLDNLAKGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QPC1</accession>
    <name>A0A481QPC1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Iron-sulfur cluster assembly scaffold protein IscU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">iscU</name>
        <name evidence="5" type="ORF">C3B55_00963</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">A scaffold on which IscS assembles Fe-S clusters. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00963"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISC_FeS_clus_asmbl_IscU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="PTHR10093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU_N" evidence="4">
        <location>
            <begin position="2"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="128" mass="13564" checksum="BEE894C1E1A4F693" modified="2019-06-05" version="1">MAYSEKIIDHYENPRNVGKMNVEDPDVGTGMVGAPACGDVMRLQIKVNAAGVIEDAKFKTYGCGSAIASSSLATERMKGKTLDEAVIISNTQLAGELALPPVKIHCSVLAEGAIKAAVRDYKQKKGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QJU7</accession>
    <name>A0A481QJU7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00017"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="48" mass="5392" checksum="DAA6FB0CADF672B9" modified="2019-06-05" version="1">MPIDASLWILVGAFLSFIRSERVQIATLAKRFTGQPQALTPIKKLDSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK07</accession>
    <name>A0A481QK07_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Polyphosphate kinase</fullName>
            <ecNumber evidence="6 7">2.7.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-polyphosphate phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Polyphosphoric acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 12" type="primary">ppk</name>
        <name evidence="12" type="ORF">C3B55_00047</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>[phosphate](n) + ATP = [phosphate](n+1) + ADP</text>
            <dbReference type="Rhea" id="RHEA:19573"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="PTM">
        <text evidence="6 7">An intermediate of this reaction is the autophosphorylated ppk in which a phosphate is covalently linked to a histidine residue through a N-P bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the polyphosphate kinase 1 (PPK1) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.1" evidence="6 7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00047"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009358">
        <property type="term" value="C:polyphosphate kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00347">
        <property type="entry name" value="Polyphosphate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003414">
        <property type="entry name" value="PP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041108">
        <property type="entry name" value="PP_kinase_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024953">
        <property type="entry name" value="PP_kinase_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036830">
        <property type="entry name" value="PP_kinase_middle_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025200">
        <property type="entry name" value="PPK_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025198">
        <property type="entry name" value="PPK_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036832">
        <property type="entry name" value="PPK_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30218">
        <property type="entry name" value="PTHR30218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02503">
        <property type="entry name" value="PP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13090">
        <property type="entry name" value="PP_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17941">
        <property type="entry name" value="PP_kinase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13089">
        <property type="entry name" value="PP_kinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015589">
        <property type="entry name" value="PP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140356">
        <property type="entry name" value="SSF140356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03705">
        <property type="entry name" value="poly_P_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 6 7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PP_kinase_N" evidence="9">
        <location>
            <begin position="54"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase" evidence="8">
        <location>
            <begin position="170"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase_C_1" evidence="11">
        <location>
            <begin position="378"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase_C" evidence="10">
        <location>
            <begin position="550"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphohistidine intermediate" evidence="6">
        <location>
            <position position="482"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="611"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="639"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="737" mass="83293" checksum="A7FCE7730FD637C9" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK34</accession>
    <name>A0A481QK34_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphomannomutase/phosphoglucomutase</fullName>
            <ecNumber evidence="12">5.4.2.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">algC</name>
        <name evidence="12" type="ORF">C3B55_00031</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.2" evidence="12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00031"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004614">
        <property type="term" value="F:phosphoglucomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="9">
        <location>
            <begin position="27"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="10">
        <location>
            <begin position="173"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="11">
        <location>
            <begin position="275"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="8">
        <location>
            <begin position="389"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="477" mass="52236" checksum="A597DBEEAD56E6AF" modified="2019-06-05" version="1">MPPFFSDRAIYAMSITTRIAPTTSNSIFRAYDIRGVVPKTLTAETSYWIGRAIGSESIAQGEPNISVGRDGRLSGPELVAQLIHGLTDSGCHISDIGLVPTPALYYAANVLAGKSGVMLTGSHNPSDYNGFKIVIAGDTLANEQIQALHDRLKNNDLTKGNGSVTKVDILQCYSDQITGDIKLARRLKVVVDCGNGVAGVIAPQLLEALNCEVIPLFCDVDGNFPNHHPDPGKPENLVDLIAKVKETSSDIGLAFDGDGDRVGVVTNTGENVFADRLLMLFARDVMARNANTEIIFDVKCTRRLTPLIKKYGGRPLMWKTGHSLIKKKMKETGALLAGEMSGHVFFKERWFGFDDGIYSAARLLEILSKEKYTAQKLFQAFPNDISTPEINISVTEESKFSIINALHNAQWGEGAHLTNIDGVRIDYAKGWGLIRASNTTPVLVLRFEADTKAELQRIKDVFFSQLRRVAPNLQLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKE8</accession>
    <name>A0A481QKE8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inner membrane protein YccS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">yccS</name>
        <name evidence="4" type="ORF">C3B55_00105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00105"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010019">
        <property type="entry name" value="Integral_membrane_YccS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010020">
        <property type="entry name" value="Integral_membrane_YCCS_YHJK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032692">
        <property type="entry name" value="YccS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12805">
        <property type="entry name" value="FUSC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01666">
        <property type="entry name" value="YCCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01667">
        <property type="entry name" value="YCCS_YHJK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="615"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="FUSC-like" evidence="3">
        <location>
            <begin position="71"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="679"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="728" mass="82033" checksum="9C9B2717DF3E9025" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKG1</accession>
    <name>A0A481QKG1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bis(5'-nucleosyl)-tetraphosphatase, symmetrical</fullName>
            <ecNumber evidence="5">3.6.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ap4A hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Diadenosine tetraphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">apaH</name>
        <name evidence="7" type="ORF">C3B55_00116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = 2 ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24252"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.41"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the Ap4A hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.41" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00116"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008803">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07422">
        <property type="entry name" value="MPP_ApaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00199">
        <property type="entry name" value="ApaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004617">
        <property type="entry name" value="ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000903">
        <property type="entry name" value="B5n-ttraPtase_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00668">
        <property type="entry name" value="apaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="6">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="280" mass="31860" checksum="F381D5DE75F9DCC9" modified="2019-06-05" version="1">MTTYAVGDLQGCLEPLKCLLQQADFNPSKDRLWLVGDLVNRGPKSLETLRFLYKIRYSLVFVLGNHDLHLLAVSSNIKNLKKGDTLHEILEAPDQAKLLDWLRQQKFIHYDKSRNIALVHAGIPPHWTLKKAMKYATEIENALIDNHIYAAYLSGIDGNKPVKLETNLTGIARLRIITNYFTRMRFCTAKGKLDLKHKGGVDTVITGYKPWFAHKERKTRNIKIIFGHWAALEGKCDEPDVFALDTGCVWGGTMTLMNIDTGERLVCHCKSFLYCNTEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKH7</accession>
    <name>A0A481QKH7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00126"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="140" mass="15374" checksum="E24BB054E2CCD1CB" modified="2019-06-05" version="1">MKARIQWIGEAMFLGESGSGHAVIMDGPPEDGGRNLSIRPMEMLLLGIGGCSNFDVVSILKKSRQAVKSCEIFLKATRATEEPKVFTKIDMHFVVKGYDLKDVQVKRAIELSAEKYCSAAIMLRAAGVVITHDYEIIGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKJ3</accession>
    <name>A0A481QKJ3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-octaprenyl-6-methoxyphenol hydroxylase</fullName>
            <ecNumber evidence="5">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ubiH</name>
        <name evidence="5" type="ORF">C3B55_00053</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00053"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008681">
        <property type="term" value="F:2-octaprenyl-6-methoxyphenol hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011295">
        <property type="entry name" value="UbiH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01984">
        <property type="entry name" value="UbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="4">
        <location>
            <begin position="5"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="395" mass="43847" checksum="42DE07038FBFCBA5" modified="2019-06-05" version="1">MSRVNLAIIGSGLVGTSLALALQAGAESRGWQILLIDQFTPGSIYQPCYDARSSALSFGVRQIYQSLGVWLNISRRAEPIKKIHVSDRGWFSTIFLSAAEEGVPAFGYVVENAWLGQCLWQGLNKEVVSWRYQMKVTRMEPLPHGYCLTLNDGTVLKCDLAVLADGGRSNLREQLGIRVKTRLYNQSSLIANITPDKAHNGEAFERFTHKGPIALLPMPDNRCMLVWTRKDIDAKRLLGLDKRSFLSELQSFFGYRLGMLKQVGTRHLYPLTLVKAEEQVRSHLVILGDAAHSLHPIAGQGFNLSMRDVSTLAEVLLAGPAVPGDLATLQNYCERQYLDQKLTVGFSNQVTRLFGSAQPLVVLSRNIGLLSLNLLPQVKHWFASHAMGMGTRPDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKJ4</accession>
    <name>A0A481QKJ4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrane fusion protein (MFP) family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">prsE</name>
        <name evidence="11" type="ORF">C3B55_00137</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00137"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006144">
        <property type="entry name" value="Secretion_HlyD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010129">
        <property type="entry name" value="T1SS_HlyD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01843">
        <property type="entry name" value="type_I_hlyD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00543">
        <property type="entry name" value="HLYD_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="10">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="250"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="454" mass="50877" checksum="FDA7B699F5518334" modified="2019-06-05" version="1">MWPDFIKNAVSRYFKSNNYLHGQPMSEINKTLIEDAPRVIRLTIWAIIAFFVFLVTWAGLSSTDEVTCGDGKAIPSSKLQKVQNLEGGIIAELYVKEGQIVEAGAPLIRLDDTRFVSNAGEIEALRLAMQLRVERLSAQVDDRPLNIHDDVLQAAPNQAANERSLYESRRQQLRDEVGGLKEQLVQRQQELREFTSKQGQYRNQLSLQRQELNMSAPLVSQGAVSPVEVLRLKRAEMETRGQLDATTLAIPRAESAIKEVQRKIDETRGKFRSDALIQLNEARTELSKAEATIRALEDRVSRTLVISPVRGIVKQLLVNTVGGVIQPGSDIVEIVPLNDTLLVEARIRPKDIAFLHPGQEAIVKFTAYDYTIYGGLQAKLERISADTMTDEDKKTTYYMITLRTDRSHLGTDDNPLLIIPGMVASVDIITGKKSILSYLLKPIIKARAEALHER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKM8</accession>
    <name>A0A481QKM8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="7">PRA-CH</shortName>
            <ecNumber evidence="7">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hisI</name>
        <name evidence="9" type="ORF">C3B55_00176</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00176"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="SSF141734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PRA-CH" evidence="8">
        <location>
            <begin position="32"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="132" mass="15378" checksum="EE56B2423AD64036" modified="2019-06-05" version="1">MQNWLNTIHWDSNDNGLVPAITQDYKTGHVLMMAWINREALCLTAAEQRVTYWSRSRGKLWRKGEQSGYMQILREMRIDCDADVVIFQVEQIGDIACHTGRYSCFYRVLENNTWKTVEPVLKDPNTIYSTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKP4</accession>
    <name>A0A481QKP4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent dethiobiotin synthetase BioD</fullName>
            <ecNumber evidence="5">6.3.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DTB synthetase</fullName>
            <shortName evidence="5">DTBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Dethiobiotin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">bioD1</name>
        <name evidence="5" type="synonym">bioD</name>
        <name evidence="6" type="ORF">C3B55_00107</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)-dethiobiotin + ADP + 3 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the dethiobiotin synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.3" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00107"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00161"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="PTHR43210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006755">
        <property type="entry name" value="DTB_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="117"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="206"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="226" mass="23858" checksum="B8D58B9C2D5A0814" modified="2019-06-05" version="1">MSAAYFITGTDTDVGKTTIATGLLYAARLVGKSTAAGKPISSGCQVTPNGLCNADAIALLAECSLPLSYAEVNPVAFKPAIAPHLAAREVGVALTVRSLLKPMQYILAQQADFTIIEGAGGWRVPLTDHDNLSDLVIALRLPVILVVSVRPGCISHSLLTAEAIVRDGLLLVGWVANIIRSKIFRLEESLATLAERLPAPCLGRVPKLKHASSAVVAKYLQLDLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKR1</accession>
    <name>A0A481QKR1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Putative protoheme IX biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">C3B55_00042</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in a late step of protoheme IX synthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Porphyrin-containing compound metabolism; protoheme biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00042"/>
    <dbReference type="UniPathway" id="UPA00252"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042168">
        <property type="term" value="P:heme metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005254">
        <property type="entry name" value="Heme_biosyn_assoc_TPR_pro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010817">
        <property type="entry name" value="HemY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07219">
        <property type="entry name" value="HemY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00540">
        <property type="entry name" value="TPR_hemY_coli"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="HemY_N" evidence="12">
        <location>
            <begin position="27"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="413" mass="47185" checksum="04FB8E37B218BBC2" modified="2019-06-05" version="1">MKRFHIILVFVIAIAIILCVSASKHTGYVLITYPHLLHYESSLWAMLVAVFAILLAIYLILVLLRLLITFSGVLNPWSRRNRIRRVQIAIENGPIDLAEGRWASAERYLHCVAESERYPLLYYLGAARAANEQGRYKESDGLLERALERQPHAELAVALSHAQLQMDRGDTDGTLVTLQAMYERYPHHAQVLRQLQRLHQQRGDWPLVIRLLSELRKDKELPDAELAELERRAWGIHLGLVIEHEEQNNAGLQYLEWSWKQLTSARRQEPQLVLTYAEQLRLLGADAKAEEVLRGAIKCGYDSYLIRLYGLLRGSDPRYQLKFAESWLEGHPGDASLLLTLGRLCLQNSLWGKAHSYFEGSLQVQRNPEACAELARLLDQLGDSKRSNQLFQESLKLLDSRLLALPLPTPTHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKR4</accession>
    <name>A0A481QKR4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal subunit assembly factor BipA</fullName>
            <ecNumber evidence="3">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GTP-binding protein BipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">typA</name>
        <name evidence="3" type="synonym">bipA</name>
        <name evidence="5" type="ORF">C3B55_00170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3">Binds to ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. BipA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00170"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03710">
        <property type="entry name" value="BipA_TypA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00849">
        <property type="entry name" value="BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035651">
        <property type="entry name" value="BipA_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042116">
        <property type="entry name" value="TypA/BipA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006298">
        <property type="entry name" value="TypA_GTP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF8">
        <property type="entry name" value="PTHR42908:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01394">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="3"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="15"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="606" mass="67551" checksum="A7CEE0C7609B1F17" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKU9</accession>
    <name>A0A481QKU9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="3">P5C reductase</shortName>
            <shortName evidence="3">P5CR</shortName>
            <ecNumber evidence="3">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">proC</name>
        <name evidence="6" type="ORF">C3B55_00086</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00086"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PTHR11645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="2 3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="4">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="P5CR_dimer" evidence="5">
        <location>
            <begin position="154"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="264" mass="28160" checksum="9B57E65B7B32A53A" modified="2019-06-05" version="1">MSNTRIAFIGAGNMAASLIGGLRDKSLEIIQISARESAKYNINTFADITEKISEVDIIVLAVKPQAMQAICKKLRPNLQPHQLVVSIAASITCASMKTWLGAAQPIVRCMPNTPALLHKGVSGLYATPEVVAKQRNQAQELLSAVGVVVWLKQEQQLDAVTALSGSGPAYFFLLIEAMITTGVKLGLPYEVTKLLVEQTALGAASMVIDSDVDTTELRRRVTSPGGTTQAALELLQAGNFEILVEKALIAAAHRSAELAKQLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKV7</accession>
    <name>A0A481QKV7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">2-oxoglutarate carboxylase small subunit</fullName>
            <ecNumber evidence="7">6.4.1.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cfiB</name>
        <name evidence="7" type="ORF">C3B55_00018</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.7" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00018"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034029">
        <property type="term" value="F:2-oxoglutarate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="6">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="119"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="471" mass="52205" checksum="9B84255C97056A0F" modified="2019-06-05" version="1">MIKKILIANRGEIAVRIIRACAEMGVRSVAVYSDADRHALHVKRADESHNVGIEPLAGYLNPRKLVNIAVETGCDALHPGYGFLSENAELADACVKRGVKFIGPSAEVIRRMGDKTEARRSMMKNGIPVIPGTEGNVADIDDALTESDRIGYPVMLKATSGGGGRGIRRCNSYEELKQAFPRVISEATKAFGSAQVFLEKCIVNPKHIEAQILGDSFGNVVHLFERDCSIQRRHQKLIEIAPSPQLTPEQRAYIGDLSVRAAKAVSYKNAGTVEFLLADGEVYFMEMNTRVQVEHTITEEITGIDIVREQIRIASGLPLSVIQEDIQHRGFALQFRINAEDPTNNFLPSFGKITRYYAPGGPGVRIDTAIYTGYTIPPFYDSICLKLVVWALTWEEAMNRGLRALDDMRLQGVKTTAAYYQEILRNPEFRSGQFNTSFVEDYPELMNYSIKRKPEELALVIATAIAAHAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL14</accession>
    <name>A0A481QL14_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="6">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Aspartate transcarbamylase</fullName>
            <shortName evidence="6">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">pyrB</name>
        <name evidence="10" type="ORF">C3B55_00089</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00089"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="9">
        <location>
            <begin position="19"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="8">
        <location>
            <begin position="171"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="334" mass="36573" checksum="53D2B0A414BE64CE" modified="2019-06-05" version="1">MIPLNAKCQLQLNAHGQLQHFLSIDGLPRRLLTEILETANSFLEVGGMAVKNIPLLNGKTICNVFFENSTRTRTTFEIAARRLSADVITLNMSTSSASKGETLLDTLRNLQAMATDMFVVRHSHSGAAHFIAEHIYPQVSIINGGDGQHAHPTQSMLDMLTIRRHKGDFENISVAIVGDILHSRVARSNMLALKTLGCPDIRVIAPKTLLPIGIGQYGVKIYTNMNQGLQDVDVVIMLRLQRERMAAGLLPSEGEFYQIFGLTTASLASAKPNAIVMHPGPINRGVEIESTLADGPQSVILNQVTYGTAVRMAVLYMAMQGQTIHCQLQQENTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL15</accession>
    <name>A0A481QL15_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="8 10">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">hisA</name>
        <name evidence="11" type="ORF">C3B55_00161</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8 10">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 8 9">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="8 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00161"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="Isoase_HisA-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00007">
        <property type="entry name" value="TIGR00007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="245" mass="26214" checksum="5DD91EF699492A10" modified="2019-06-05" version="1">MLIIPAIDLKNGACVRLRQGRMEDFTVFSDDPVSMAGKWVEKGCRRLHLVDLNGAFEGYPVIGEVVAAIAKRYHSLPIQIGGGIRSLETIEYYVKAGVSYVIIGTKAVKDLAFVAEACRTFPGKIIVSLDAKDSFVATDGWAEISTVQAIVLANQLEAYGVSAIVYTDISRDGMMQGCNILFTIALAAATKIPVIASGGIRNLGDIKSLLDANVPSIIGAITGRAIYEGTLDITEAQAYCDTYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL27</accession>
    <name>A0A481QL27_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutathione synthetase</fullName>
            <ecNumber evidence="10">6.3.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GSH synthetase</fullName>
            <shortName evidence="10">GSH-S</shortName>
            <shortName evidence="10">GSHase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutathione synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">gshB</name>
        <name evidence="13" type="ORF">C3B55_00092</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + glutathione + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the prokaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00092"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00162">
        <property type="entry name" value="GSH_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006284">
        <property type="entry name" value="Glut_synth_pro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004218">
        <property type="entry name" value="GSHS_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004215">
        <property type="entry name" value="GSHS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF4">
        <property type="entry name" value="PTHR21621:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02955">
        <property type="entry name" value="GSH-S_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02951">
        <property type="entry name" value="GSH-S_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01380">
        <property type="entry name" value="glut_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="3 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="125"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="317" mass="35815" checksum="B0C20027B90B0298" modified="2019-06-05" version="1">MNVRGGIVMDPIAHITYNKDSSLAILLAAQSSGWTLFYMEQRDLYYGDGEVRARMRQLQVFPNHEKWFELDNEIDSRLSDFDVILMRKDPPFNMEFIYSTYLLEQAERVGVLIVNKPQSLRDYNEKLFVTFFPQCAPPTVVSRHPDVLRKFASNHSDVILKPLDGMGGTSIFRHRAGDPNLSVILETMTALGTQQIMGQTYLPAIKDGDKRILMIDGEPVDYFLARIPAAGETRGNLAAGGHGEARILSDKDRWIASQVGPTLREKGLLFVGLDIIGEHLTEINVTNPTCIREIDNAFDINISEMLIETIERKLQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL46</accession>
    <name>A0A481QL46_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">dnaK</name>
        <name evidence="10" type="ORF">C3B55_00358</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a chaperone.</text>
    </comment>
    <comment type="induction">
        <text evidence="7">By stress conditions e.g. heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00358"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="SSF100934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="610"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="621"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine; by autocatalysis" evidence="7">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="638" mass="68681" checksum="002C33F76FB039DA" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL59</accession>
    <name>A0A481QL59_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase EpsD</fullName>
            <ecNumber evidence="3">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">epsD</name>
        <name evidence="3" type="ORF">C3B55_00204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00204"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="19"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="148"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="339" mass="37759" checksum="EFB9338FCB436C93" modified="2019-06-05" version="1">MTTVFLTGSSDPTVVASCDSDEVLFMEFSSKDIRGLKLCAICELHKIATSRNFSLCIAHRFKPIYIALIGTRLPVIGVHHAFSDYQRCSRKLLAWIFRKRLSLLGVSNAVRDDMRRSLPALPAVRIQTLYNRIDIEAMQALQVSAHEARDVLGLSLDEWVIGNVGRLHPDKDQATLLKGFALALPHLPARSRLAILGTGYLEQDLKSLACKLGIAERVLFFGYVPDARRYFHAFDTFVLSSNNEPFGMVLLEAMVAGVPLLATSCGGSREVVDGVGTLFPLGDAEHLGNGLLHLAAMNRQEHKQYSDMILKSLRQRFSDQVARDTFWQLPHVTELITRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL60</accession>
    <name>A0A481QL60_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
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            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00357"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="37" mass="4130" checksum="C22FDA9D2E6F06C5" modified="2019-06-05" version="1">MLHHLLMSRLEISCNDPTYKFKRLSATAVIPTAAATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL77</accession>
    <name>A0A481QL77_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00224"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="46" mass="5044" checksum="11DCF19756AB56BD" modified="2019-06-05" version="1">MKPAAILLQLMWSKLNAQMSGEANQFTATVEVEQLRFSSSVCTLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLA5</accession>
    <name>A0A481QLA5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Pyruvate dehydrogenase E1 component</fullName>
            <ecNumber evidence="4 10">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">aceE</name>
        <name evidence="14" type="ORF">C3B55_00196</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H(+) + pyruvate = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10480"/>
            <dbReference type="Rhea" id="RHEA-COMP:10481"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer. Part of the PDH complex, consisting of multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="4 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00196"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02017">
        <property type="entry name" value="TPP_E1_EcPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035807">
        <property type="entry name" value="PDC_E1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004660">
        <property type="entry name" value="PDH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041621">
        <property type="entry name" value="PDH_E1_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825:SF3">
        <property type="entry name" value="PTHR43825:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17831">
        <property type="entry name" value="PDH_E1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000156">
        <property type="entry name" value="Pyruvate_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00759">
        <property type="entry name" value="aceE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="12">
        <location>
            <begin position="183"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="PDH_E1_M" evidence="13">
        <location>
            <begin position="476"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000156-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="881" mass="99868" checksum="A3A8A32A5D9B2FE4" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLB8</accession>
    <name>A0A481QLB8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00189"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="48" mass="5415" checksum="A94DD4A9F02F1BEE" modified="2019-06-05" version="1">MVVIIMSRSRDYKVILSGTVDYNLQAQFKLSRCGLSSFKSMLICINAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLE9</accession>
    <name>A0A481QLE9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Adenylosuccinate lyase</fullName>
            <shortName evidence="11">ASL</shortName>
            <ecNumber evidence="4 11">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">purB</name>
        <name evidence="14" type="ORF">C3B55_00448</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="4 11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00448"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013539">
        <property type="entry name" value="PurB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08328">
        <property type="entry name" value="ASL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="12">
        <location>
            <begin position="14"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="ASL_C" evidence="13">
        <location>
            <begin position="332"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="456" mass="51038" checksum="B18D325716815488" modified="2019-06-05" version="1">MQISSLTAISPIDGRYANKTQALRYIFSEYGLIRARILVEVRWLQRLAAHPAISEVLVFSDKANAILSALTENFSLKYAERVKEIECSTNHDVKAVEYLLKEQAATVPELAKVSEFIHFACTSEDINNLAYAMMLKEGRHDIILPLMRQTAEAIRELAINFATVSMLSRTHGQPASPTTLGKELANVVYRLERQIAQTAAVPLMGKINGAVGNYNAHLSAYPDIDWEANSRIFIEDELGLCFNPYTTQIEPHDYIAELCNAIARFNIILIDFNRDIWGYISLGYFKLHIITSESGSSTMPHKINPINFENSEGNLGMANAVLHHLASNLPISRWQRDLTGSTVLRNLGVGLAYCVIAYKASLKGISQLELNEQKIAADLNSFWEVLAEPIQTVMRRYNIENPYEKLKELTRGKVISPDALQTFIDNLDIPVSAKIKLKLLTPANYIGNAVTQAKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLF4</accession>
    <name>A0A481QLF4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">DNA-directed RNA polymerase subunit beta</fullName>
            <shortName evidence="6">RNAP subunit beta</shortName>
            <ecNumber evidence="6 8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">RNA polymerase subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Transcriptase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 15" type="primary">rpoB</name>
        <name evidence="15" type="ORF">C3B55_00270</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6 8">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="6 8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00270"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01321">
        <property type="entry name" value="RNApol_bact_RpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042107">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_ext_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019462">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_external_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010243">
        <property type="entry name" value="RNA_pol_bsu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10385">
        <property type="entry name" value="RNA_pol_Rpb2_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02013">
        <property type="entry name" value="rpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA_pol_Rpb2_1" evidence="12">
        <location>
            <begin position="26"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_2" evidence="11">
        <location>
            <begin position="156"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_2" evidence="11">
        <location>
            <begin position="359"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_3" evidence="13">
        <location>
            <begin position="518"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_45" evidence="14">
        <location>
            <begin position="596"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_6" evidence="9">
        <location>
            <begin position="722"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_7" evidence="10">
        <location>
            <begin position="1281"/>
            <end position="1356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX81625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="1357" mass="151405" checksum="E4FD9ECBD4E753E0" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLF7</accession>
    <name>A0A481QLF7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoyl synthase</fullName>
            <ecNumber evidence="6">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Lip-syn</fullName>
            <shortName evidence="6">LS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Lipoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Lipoic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Sulfur insertion protein LipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">lipA</name>
        <name evidence="8" type="ORF">C3B55_00332</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00332"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102552">
        <property type="term" value="F:lipoyl synthase activity (acting on glycine-cleavage complex H protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102553">
        <property type="term" value="F:lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="PTHR10949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="95"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="336" mass="37705" checksum="573F745731143F79" modified="2019-06-05" version="1">MTTDSAQNIIMTLDITERPALRDTAEVGVQLRGAAKVARIPIKVIQATELLKKPTWICVRISASPAVDHVKALLRKYNLYSVCEEASCPNLGECFSSGTATFMIMGNICTRRCPFCDVSHGRPKPLDVQEPESLAIAITNLKLKYVVITSVNRDDLRDGGAQHFADCIREIRKLSPHIMLETLVPDYRGRMDVALAITVSEPPDVFNHNLETVPRLYKAVRPGSDYQWSLTMLQKFKQMMPHIPTKSGLMLGLGETDEEVISVMKCMREHDIDMLTLGQYLQPSRSHLPVQRFVHPSTFVYFAEEGYKMGFKNVASGPLVRSSYHADKQVNLVRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLG7</accession>
    <name>A0A481QLG7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="7">EPSP synthase</shortName>
            <shortName evidence="7">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">aroA</name>
        <name evidence="9" type="ORF">C3B55_00472</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00472"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="13"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="26"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="7">
        <location>
            <begin position="95"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="170"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="418" mass="44546" checksum="CDEC3607D445244B" modified="2019-06-05" version="1">MSSQKTVTVTPPKSPINGKVLLPGSKSITNRVLLLAALAKGTSRLSGALKSDDTNYMSIALRQMGVTIDEPDNTTFVVTGSGKLQMPTQPLFLGNAGTAMRFLTAASVLVEGIVVQNGDNNMHTRPISPLLAALSQNGIRVDSQTGYPPVIVHGVGKMHAKRFEIDGSLSSQYVSALLMLAACGEAPAEIVLTGKDISANGYIDLTLNCMRAFGAKIKTVNDTTWHVAPTGYTARNYLIEPDASAATYLWAAEVLTGGRIDIGIDAPDFTQPDAKAKAIITQFPNIQTIVVGSKIQDAIPTLAVLAAFNNTPVRFTELTNLRIKECDRVQALYNGLNKICHGLASIDGDDLLIASDPTLANISSNALIDTQTDHRIAMCFALAGLKITGIRIQNPGCVAKTYPEYWKTLVSLGVVLCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLH6</accession>
    <name>A0A481QLH6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosome association toxin RatA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ratA</name>
        <name evidence="4" type="ORF">C3B55_00353</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribosome association toxin RatA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00353"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="PTHR12901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="Polyketide_cyc" evidence="3">
        <location>
            <begin position="11"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="150" mass="16822" checksum="2799163C0AEB592F" modified="2019-06-05" version="1">MTMHIKRSAVLPYSAQALYELVNDVARYPEFLPWCSTAEVLEGSDEYMVASVGIVNGGFSQHFVTRNVLVFGKSIEMNLQEGPCTQLHGVWVFKSLTDKACKISLDLSFDYSGPIVRVILRPLFHYVANTLVDAFCQRPMQLHRGDCSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLJ7</accession>
    <name>A0A481QLJ7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="7">3.3.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ahcY</name>
        <name evidence="7" type="ORF">C3B55_00098</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EC" id="3.3.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00098"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="PTHR23420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AdoHcyase_NAD" evidence="6">
        <location>
            <begin position="199"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="469" mass="52171" checksum="6A3C8F1C7993932D" modified="2019-06-05" version="1">MSVAKTRVDFNDYKVANISLAAWGRREIIIAEFEMPALMGLRRKYATEQPLQGAKILGCIHMTIQTAVLIETLVALGAEVRWSSCNILSTQDQAAAAIAATGIAVYAWKGATEQEYQWCLEQTILKDGKLWDANMVLDDGGDLTELLHKKYPKTLDRIHGVTEETTTGVYRLLNMLDKGELRIPAINVNDSVTKSKNDNKYGCRHSLNDAIKRGTDHLLSGKQTLVIGYGDVGKGSSQSLRQEGMIVKVSEVDPICAMQACMDGFEVVSPFVNGYNDDTEASIDKALLNKIDLIVTTTGNMHVCNAHMLKALKKRAIICNIGHFDNEIDIAFMRKNWIWEEVKPQVHKVHRANDGNVDPQNDDYLILLAEGRLVNLGNATGHPSRVMDGSFANQVLAQIFLFRQKYADLTLSQKAERLTVEVLPKKLDEEVAMEMVRGFGGMLTYLTKTQANYIGVQIGGPFKPQAYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLJ9</accession>
    <name>A0A481QLJ9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00548"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="129" mass="14691" checksum="DC39B948355DC036" modified="2019-06-05" version="1">MSSAISLKQYSQLVLYNIMSHFSVSIFGTWRYITFKVYPLVYSITNYTFAYNLNCIDKLIGMAALPSIVLLPKLGIGDFKSFFWIARLAMSKHGLSSRLTMYSFKDYEDLVYARMMLLACAATAVDVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLM3</accession>
    <name>A0A481QLM3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 19">UDP-N-acetylenolpyruvoylglucosamine reductase</fullName>
            <ecNumber evidence="6 19">1.3.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 19">UDP-N-acetylmuramate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 21" type="primary">murB</name>
        <name evidence="21" type="ORF">C3B55_00468</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 19">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:12248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="EC" id="1.3.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 19">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 19">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the MurB family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.98" evidence="6 19"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00468"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008762">
        <property type="term" value="F:UDP-N-acetylmuramate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.78.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00037">
        <property type="entry name" value="MurB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003170">
        <property type="entry name" value="MurB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011601">
        <property type="entry name" value="MurB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036635">
        <property type="entry name" value="MurB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21071">
        <property type="entry name" value="PTHR21071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02873">
        <property type="entry name" value="MurB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56194">
        <property type="entry name" value="SSF56194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00179">
        <property type="entry name" value="murB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9 19" id="KW-0132">Cell division</keyword>
    <keyword evidence="13 19" id="KW-0133">Cell shape</keyword>
    <keyword evidence="17 19" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 19" id="KW-0274">FAD</keyword>
    <keyword evidence="10 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12 19" id="KW-0521">NADP</keyword>
    <keyword evidence="15 19 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14 19" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="20">
        <location>
            <begin position="16"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="19">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="QAX81808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="339" mass="37393" checksum="6A894A7FBADD9BA1" modified="2019-06-05" version="1">MQVFAQISLKPFNSFGIDVCAQLFAEAYDDSDVHEAISYAVIHALPLFIIGGGSNLLLTQDIHALVLRMATQGIRVLQDDSVHVVIEAEAGEAWHPFVLWTLDHGLYGLENLSLIPGTVGAAPMQNIGAYGVEIKDVFAGLTALDCQTGELRDFSLQECQFAYRDSLLKHDIGRWLVLRVRFMLSRISNKKLDYGSLQQCLVKQGITEATPIDISRAICSIRRETLPDPVKLGNAGSFFKNPLVSEVLAAELKCQYPDLVTHQQPDGQIKLAAGWLIDKSGWKGFRDGDAGVYKMQALVLVNYGAATGRDIVSLAKRIQSDIAKRFKVHLEMEPTIIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLN6</accession>
    <name>A0A481QLN6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="9">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 15" type="primary">leuS</name>
        <name evidence="15" type="ORF">C3B55_00335</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00335"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_B">
        <property type="entry name" value="Leu_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="PTHR43740"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1g" evidence="13">
        <location>
            <begin position="39"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1_2" evidence="14">
        <location>
            <begin position="221"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1" evidence="11">
        <location>
            <begin position="423"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="12">
        <location>
            <begin position="707"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="627"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="630"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX81690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="868" mass="98371" checksum="3035947F691DECF4" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLN7</accession>
    <name>A0A481QLN7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide chain release factor 2</fullName>
            <shortName evidence="4">RF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">prfB</name>
        <name evidence="6" type="ORF">C3B55_00500</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="4">Methylated by PrmC. Methylation increases the termination efficiency of RF2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00500"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00094">
        <property type="entry name" value="Rel_fac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004374">
        <property type="entry name" value="PrfB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00020">
        <property type="entry name" value="prfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0488">Methylation</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="5">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="4">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="348" mass="39073" checksum="DC9EA77988178CE0" modified="2019-06-05" version="1">MKLFGGVFDYSQKHERLTEINRELEDPNVWNKPEYAQELGRERALLAQIVDTLDELHIGLCDCRDLLEMAITENDEGAAGDVSTYLASLEESLAKLEFNRMFSHEIDSNNAYLNIQAGSGGTEAQDWANILLCMYLRWADKRGFDATIMELSAGEVAGIKGVTVHIKGEYAFGWLRTEIGVHRLVRKSPFDSGNRRHTSFSAVFVSPEVDDKVELEINLADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTNTVVSCQNERSQHANKDTAMKMLRAKLYEQEMQKRNAALQALEGTKSDIGWGHQIRSYVLDASRIKDLRTNIERSDCNKVLDGDIDEYLEASLKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLP2</accession>
    <name>A0A481QLP2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NYN domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00345"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="64"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="219" mass="24439" checksum="A487B2D257DFA501" modified="2019-06-05" version="1">MVFITTIAMIFFFLNSEDNHLHLISYNYNALLPKINWLRNVSVSSILVFSGVSARLSRGSAVKKIAVFADVQNLYYTVRQAYGCHFNYAALWADISMRGQIVEAYAYAIDRGDSKQQQFQQILRNLGFMVKLKPYIQRADGSAKGDWDVGITLDIMDTIDHVDEVVLASGDGDFNMLLERITYKHGVEAVAYGVPGLTASSLVRAASRYVPIEGALLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLP4</accession>
    <name>A0A481QLP4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase-binding transcription factor DksA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">dksA</name>
        <name evidence="8" type="ORF">C3B55_00383</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Transcription factor that acts by binding directly to the RNA polymerase (RNAP). Required for negative regulation of rRNA expression and positive regulation of several amino acid biosynthesis promoters. Also required for regulation of fis expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts directly with the RNA polymerase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DksA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00383"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00926">
        <property type="entry name" value="DksA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012784">
        <property type="entry name" value="DksA_RNA_pol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037187">
        <property type="entry name" value="DnaK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020458">
        <property type="entry name" value="Znf_DskA_TraR_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01258">
        <property type="entry name" value="zf-dskA_traR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109635">
        <property type="entry name" value="SSF109635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02420">
        <property type="entry name" value="dksA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01102">
        <property type="entry name" value="ZF_DKSA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51128">
        <property type="entry name" value="ZF_DKSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DksA C4-type" evidence="7">
        <location>
            <begin position="66"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="dksA C4-type" evidence="6">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="146" mass="17151" checksum="7909D75EB59D9852" modified="2019-06-05" version="1">MSTQTKQQQQSLSGFIPYVETEGEKYMSNPMRQHFTRILQKWKQNLMQEVDRTVDHMKDEAANFPDPADRASQEEEFALELRARDRERKLIKKIDKTLQLIENEEYGWCESCDVEIGIRRLEARPTADLCVDCKTLAEIKEKQVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLR2</accession>
    <name>A0A481QLR2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Release factor glutamine methyltransferase</fullName>
            <shortName evidence="6">RF MTase</shortName>
            <ecNumber evidence="6">2.1.1.297</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">N5-glutamine methyltransferase PrmC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein-(glutamine-N5) MTase PrmC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein-glutamine N-methyltransferase PrmC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">prmC</name>
        <name evidence="9" type="ORF">C3B55_00409</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = H(+) + N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00409"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:protein-(glutamine-N5) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036009">
        <property type="term" value="F:protein-glutamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018364">
        <property type="term" value="P:peptidyl-glutamine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02126">
        <property type="entry name" value="RF_methyltr_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="Release_fac_Glu-N5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PrmC_N" evidence="8">
        <location>
            <begin position="5"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="MTS" evidence="7">
        <location>
            <begin position="109"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="116"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="182"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="276" mass="30712" checksum="8269AF4657DC1255" modified="2019-06-05" version="1">MTIIASLLRAANTLHSPTARLDVELLLSAILGKSRSYLHTWPEKIVCGEDVETFTNNLQRRRTGEPVAYILGQQFFWKLDLEVTPHTLIPRPETELLVDAALELPLLDPVKLLDLGTGSGAIALALASERQAWHVTAVDRVLEAVALAERNRQRLHLKNATVLNSYWFSALQGHTFDLIVSNPPYIAGNDPHLLSGDVRFEPASALVAGHDGLEDLRVIITKSVVYLNVDGWLLLEHGYNQAVIVRDLLLSEGFEDVHSRMDLGGHERITLGRRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLR3</accession>
    <name>A0A481QLR3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polyribonucleotide nucleotidyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Polynucleotide phosphorylase</fullName>
            <shortName evidence="7">PNPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">pnp</name>
        <name evidence="9" type="ORF">C3B55_00378</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate + RNA(n)</text>
            <dbReference type="Rhea" id="RHEA:22096"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the polyribonucleotide nucleotidyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.8" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00378"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004654">
        <property type="term" value="F:polyribonucleotide nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01595">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012162">
        <property type="entry name" value="PNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015848">
        <property type="entry name" value="PNPase_PH_RNA-bd_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036456">
        <property type="entry name" value="PNPase_PH_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252">
        <property type="entry name" value="PTHR11252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03726">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005499">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46915">
        <property type="entry name" value="SSF46915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03591">
        <property type="entry name" value="polynuc_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="623"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="487"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="701" mass="75601" checksum="030D8AA7E9D65DBA" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLR4</accession>
    <name>A0A481QLR4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Exodeoxyribonuclease III</fullName>
            <ecNumber evidence="4">3.1.11.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">xthA</name>
        <name evidence="4" type="ORF">C3B55_00599</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00599"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008853">
        <property type="term" value="F:exodeoxyribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09086">
        <property type="entry name" value="ExoIII-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037493">
        <property type="entry name" value="ExoIII-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43250">
        <property type="entry name" value="PTHR43250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00727">
        <property type="entry name" value="AP_NUCLEASE_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="270" mass="31266" checksum="87D150DD0A2BB5D5" modified="2019-06-05" version="1">MKIVSFNINGLRARRHQLEALIEKHQPDVIGLQETKVHDDQFPLAEVQDLGYQVYYYGQKCHYGVAILSRKEFLNVHKGFASDKENTQRRFIWGIITDENDNSIIIMNGYFPQGESRKHPIKFPAKQRFYADLQQLLESNFSNNQALIVMGDVNISPEDSDIGIGIGNAKRWLKNGKCSFLPEEREWMVRLKNWGLVDSFRHLTPDVNNCFSWFDYRSRGFEDEPKRGLRIDVILASTGLVPRLKEAGVDYELRGLEKPSDHAPIWLELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLS0</accession>
    <name>A0A481QLS0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Riboflavin biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">Riboflavin kinase</fullName>
                <ecNumber evidence="12">2.7.1.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="12">Flavokinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">FMN adenylyltransferase</fullName>
                <ecNumber evidence="12">2.7.7.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="12">FAD pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="12">FAD synthase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="14" type="primary">ribF</name>
        <name evidence="14" type="ORF">C3B55_00419</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the phosphorylation of riboflavin to FMN followed by the adenylation of FMN to FAD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 12">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + riboflavin = ADP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 12">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ribF family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="12"/>
    <dbReference type="EC" id="2.7.7.2" evidence="12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00419"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02064">
        <property type="entry name" value="FAD_synthetase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015864">
        <property type="entry name" value="FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002606">
        <property type="entry name" value="Riboflavin_kinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="PTHR22749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06574">
        <property type="entry name" value="FAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004491">
        <property type="entry name" value="FAD_Synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00083">
        <property type="entry name" value="ribF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0274">FAD</keyword>
    <keyword evidence="6 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 12" id="KW-0288">FMN</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavokinase" evidence="13">
        <location>
            <begin position="189"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR004491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="314" mass="34736" checksum="FBEE469FC4493C17" modified="2019-06-05" version="1">MSKKRFMQLVRGLHNLRPEHRGCVATIGNFDGVHRGHQALLGRLRKRAVELGVPSCVVIFEPQPQEFFIPSMAPARLACLRDKQQLLTEEGVSHVLCLAFNQLLQSFSAVEFIDRILVHGLGVKHLEVGDDFRFGYDRDGDFNFLQNAGMSQGFTVEAAQTVKLDGVRISSTQVRNALGAADFAMAKRLLGRQFSIAGRVLRGQKLARQLGAPTANVQLKRRRVPLAGVYLVSVDIDGQSWPGVANIGVRPTVISDGAAHLEVHLLDFVGDLYDRHLTVVFHHKLREEQCFASLEALKIMINADVATARILSCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLS2</accession>
    <name>A0A481QLS2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00612"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="73" mass="8315" checksum="3902F254C9857C64" modified="2019-06-05" version="1">MPAVPSGYITYLAASNTTGNCTYSTAKLKSARFTRHPYKEVTSVLTSDIEATEDWIVKVSKRDQKIIFWQFHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLT3</accession>
    <name>A0A481QLT3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00563"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="32" mass="3668" checksum="B7D7DAF833A66C72" modified="2019-06-05" version="1">MTTVDFLYITKQITQLPHTWTLPAGLPKKPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLT8</accession>
    <name>A0A481QLT8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aminopeptidase</fullName>
            <ecNumber evidence="5">3.4.11.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">C3B55_00634</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00634"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="3">
        <location>
            <begin position="14"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="2">
        <location>
            <begin position="317"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24_C" evidence="4">
        <location>
            <begin position="542"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="602" mass="66420" checksum="4C93E66AF8B794F5" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLU5</accession>
    <name>A0A481QLU5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00646</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00646"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="38" mass="4443" checksum="18F98C740E9AD3CB" modified="2019-06-05" version="1">MFPLIHFYEHIITITNTNKLDISIKQQDLIECIKGQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLU6</accession>
    <name>A0A481QLU6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="2">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="2">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 7" type="primary">sucD</name>
        <name evidence="7" type="ORF">C3B55_00657</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00657"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA_binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2 3">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="289" mass="29713" checksum="965E4E6E6928AC9D" modified="2019-06-05" version="1">MSVLINKDTKVICQGITGAQGSFHTQQAIEYGTKMVGGVSPSKGGTKHLNLPVFNTVKDAVAITGATASVIYVPAPFCKDAILEAAFGGIKLIVCITEGIPTLDMLTVTVICDELNVVLIGPNCPGVITPGECKIGIMPGHIHLPGKVGIVSRSGTLTYEAVKQTTDAGFGQSTCVGIGGDSILGSTFIDILKLFQGDSKTEAIVMIGEIGGSAEEEAATYIKRSVTKPVVAYIAGVTAPAGRRMGHAGAIIYGGEGTADKKFVVLQDAGVKIVRSLAAIGIALADLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLU8</accession>
    <name>A0A481QLU8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NADH-quinone oxidoreductase subunit L</fullName>
            <ecNumber evidence="9">1.6.5.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nuoL</name>
        <name evidence="9" type="ORF">C3B55_00437</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.5.11" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00437"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018393">
        <property type="entry name" value="NADHpl_OxRdtase_5_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="PTHR42829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01974">
        <property type="entry name" value="NDH_I_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="594"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_N" evidence="8">
        <location>
            <begin position="67"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="7">
        <location>
            <begin position="134"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="615" mass="67125" checksum="D21C30AB086BD442" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLV7</accession>
    <name>A0A481QLV7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gltA</name>
        <name evidence="9" type="ORF">C3B55_00666</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5 8">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00666"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00717"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004108">
        <property type="term" value="F:citrate (Si)-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06114">
        <property type="entry name" value="EcCS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024176">
        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010953">
        <property type="entry name" value="Citrate_synthase_typ-I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01798">
        <property type="entry name" value="cit_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001369-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="424" mass="47186" checksum="FF717C79E8CAFDC6" modified="2019-06-05" version="1">MADKTAQLIIEGAAPVELPILTSTVGPDVIDVRGLTATGHFTFDPGFMSTASCESKITYIDGDHGILLYRGYPIEQLAEKSDYLETCYLLLNGELPTNKQKAKFVGAVKNHTMVHEQLKTFFNGFRRDAHPMAIMCGVVSALSAFYHDSLDINNQQHREISAIRLVAKIPTLAAMVYKYSMGQPMMYPRNDLTYTENFLHMMFNTPCAIKPISSVLAAALDKIFILHADHEQNASTSTVRLTGSSGANPFACIAAGIAALWGPAHGGANEAVLTMLDEISHVSNIDKFIAKAKDKNDLFKLMGFGHRVYKNRDPRATVMKKTCDEVLKELGIQNDPQLELAIRLEEIALTDSYFIERSLYPNVDFYSGIIFKAIGIPTSMFTVIFALARTVGWISHWKEMLSSPYKIGRPHQLYTGYKPRDFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLX8</accession>
    <name>A0A481QLX8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH-quinone oxidoreductase subunit I</fullName>
            <ecNumber evidence="6">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">NADH dehydrogenase I subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">NDH-1 subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">nuoI</name>
        <name evidence="8" type="ORF">C3B55_00440</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00440"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="PTHR10849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="6" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="182" mass="20647" checksum="5C5B9FD3C004C29F" modified="2019-06-05" version="1">MFKYISDIVKGTGTQLRSLLMVFSHSFHKRDTLQYPEEAVYLPPRYRGRIVLTRDPNGEERCVACNLCAVACPVDCISLQKAETEDGRWYPDFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMAEFDRQDLVYEKKDLLISGPGKNPNYNFYHVTGMAISGKSKGNAQNEAEPINVKSLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLZ2</accession>
    <name>A0A481QLZ2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal silencing factor RsfS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">rsfS</name>
        <name evidence="3" type="ORF">C3B55_00324</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with ribosomal protein L14 (rplN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00324"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01477">
        <property type="entry name" value="Iojap_RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="PTHR21043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00090">
        <property type="entry name" value="rsfS_iojap_ybeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="149" mass="16758" checksum="27C6ACA8D45A8A24" modified="2019-06-05" version="1">MKNKDVSKVMRKSIFKSAILPVKRYADYELPSEALVKVAVTALEEVKAKNIEILDIRDKQGIIDFMIIATGTSNRQLCAMLDKVSEAVKAQGVQPLGEEGKDNSDWVLLDVDNVIIHMMTSIARQFYDLEGLWNSAEKSCAVDVKDYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM02</accession>
    <name>A0A481QM02_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00641</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00641"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="31" mass="3673" checksum="A8617F39E77EEF38" modified="2019-06-05" version="1">MVLLVVFYFFKQVYSMYAAKTGYYTGINILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM06</accession>
    <name>A0A481QM06_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="8">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">trpS</name>
        <name evidence="10" type="ORF">C3B55_00541</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00541"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_B">
        <property type="entry name" value="Trp_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024109">
        <property type="entry name" value="Trp-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036913">
        <property type="entry name" value="YegP-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160113">
        <property type="entry name" value="SSF160113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="13"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="206"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="20"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="206"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="456" mass="50549" checksum="E8785476A03B5F5B" modified="2019-06-05" version="1">MTIRTHILTGITTTGTPHLGNYAGVIRPAIIASQDANTDSFYFLADYHALIKCNDPQRTKNSRMQIAATWLACGLDVNKVTFYRQSDIPEILELTWLLTCIASKGLLNRAHAYKASVDRNIENGEDQDAGISMGLYSYPVLMAADILMFNAHKVLVGRDQIQHIEMARDIGQRFNHLFGKGKECFTIPEALIENSVATLPGLDGRKMSKSYGNTIPLFTGAKDMMDAILRIVTDSSAPGKAKNPDSSHLFTLFQAFASKTQKEEFRAELLQGLSWSEAKNRLFQLLDCHLGEARERYHQLMSRPSDMEDFLLLGAKKARAVAIPFLAEIREAVGICSFVKQTVIPIEAKRNPIKVAHFVSFRENDGSFRFRLLAADGEQLLLSCKFVDGKAANAGIKHLQSGNTLDLRIEATTFSVWLSDVMVACSATFADNTSRDMAITSLRVALASIENYQAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM07</accession>
    <name>A0A481QM07_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00543</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00543"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="50" mass="5730" checksum="60C83DC49B2FFC52" modified="2019-06-05" version="1">MNIMVLRNKPCSGTEIYIELIISRVFCLLIKQIALFKYKNRLACDSGLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM11</accession>
    <name>A0A481QM11_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Ribonuclease H</fullName>
            <shortName evidence="6">RNase H</shortName>
            <ecNumber evidence="4 6">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">rnhA</name>
        <name evidence="8" type="ORF">C3B55_00487</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="4 6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00487"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09278">
        <property type="entry name" value="RNase_HI_prokaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00042">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022892">
        <property type="entry name" value="RNaseHI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="150" mass="17079" checksum="F07E1F70173A2DCA" modified="2019-06-05" version="1">MTDTVKLFTDGACKGNPGPGGWGALLVCKGVEMELWGGAVNTTNNRMELMGTIRGLEKLKRRCDVLLVTDSQYVMKGTNEWIANWKKRGWKTVTKEPVKNADLWQLLDEQCNRHNVIWKWVRGHIGYLGNERADQLANRGVDELLCYKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM18</accession>
    <name>A0A481QM18_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxoglutarate dehydrogenase (succinyl-transferring)</fullName>
            <ecNumber evidence="4">1.2.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sucA</name>
        <name evidence="8" type="ORF">C3B55_00661</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">E1 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the decarboxylation of 2-oxoglutarate, the first step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00661"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="PTHR23152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transket_pyr" evidence="7">
        <location>
            <begin position="599"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
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</entry>
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        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="36" mass="4550" checksum="DDF90D13FD097550" modified="2019-06-05" version="1">MGYMYRQNIEFLLFDIFKLKQSKNDDNFYYDIESFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM47</accession>
    <name>A0A481QM47_9PSED</name>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
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            </authorList>
            <dbReference type="PubMed" id="29860412"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="658" mass="72755" checksum="09B6380219141572" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM63</accession>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="QAX81769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="77" mass="8881" checksum="CC2D3AFB7310BC1A" modified="2019-06-05" version="1">MVSLLFWIAVITAAVSFWRKFIHQAAKQQNPNKFNTVLMVRCANCGLHLPQDCALNSRQEWYCNQAHLEQGPKSIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM64</accession>
    <name>A0A481QM64_9PSED</name>
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            <fullName evidence="8">Cytidylate kinase</fullName>
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        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
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        <lineage>
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            <taxon>Gammaproteobacteria</taxon>
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            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
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            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
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            <text>ATP + CMP = ADP + CDP</text>
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        </reaction>
    </comment>
    <comment type="catalytic activity">
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            <text>ATP + dCMP = ADP + dCDP</text>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the cytidylate kinase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00693"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00238">
        <property type="entry name" value="Cytidyl_kinase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003136">
        <property type="entry name" value="Cytidylate_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02224">
        <property type="entry name" value="Cytidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00017">
        <property type="entry name" value="cmk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidylate_kin" evidence="9">
        <location>
            <begin position="8"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="229" mass="25252" checksum="79DE29BE1A935D55" modified="2019-06-05" version="1">MNIKVPVITIDGPSGSGKGTIAGLLARRLGWCLLDSGALYRLLALAARNHGVDLANEKCMKLLAAHLDVRFVGAMEDRPQRIILESKDVTDDLRNNQIDSWSSCIAELPAVRNVLLQRQRAFQEPPGLVADGRDMGTVVFPGAPLKIFLTASVEERARRRYLQLKGKVDDVILCRVLNEIRARDERDIRRVIAPLKPAADAIYLNSTALSVEQVLKLIVSEIAIRDIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM65</accession>
    <name>A0A481QM65_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxylesterase 2</fullName>
            <ecNumber evidence="2">3.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">estB</name>
        <name evidence="2" type="ORF">C3B55_00567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00567"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080030">
        <property type="term" value="F:methyl indole-3-acetate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_2" evidence="1">
        <location>
            <begin position="4"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="218" mass="24135" checksum="DB55216FCE79F06D" modified="2019-06-05" version="1">MTEPLILQPTKPADACVIWLHGLGADQYSFLPAAKLLQETLLSMRFVLPQAPTRQVTINGGYKIPSWYDITAMSPARSINLAELEESANMVTDLIRAQEKTGIDVSRIFLAGFSQGGAVVFHTAYLGWQEPLGGVIAISTYAPTFNKMLKLSTNQQHIPVLCLHGSYDEVVLYAMGLSAFTHLKSRDVIATWHEYPIGHKVLPEEIRDIGNWLTVRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM72</accession>
    <name>A0A481QM72_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00819"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="108" mass="12024" checksum="4A28FD63159227EE" modified="2019-06-05" version="1">MKIYDSFDIRRLRPKIQSNWHLHLAASFAALLVIVGLLLAGAGGVVGLFGHFLAFSLPSGNHSGSAILLVAGLLVLWLGIYLWRRSRRRIHQALSLNIASHLLKKYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM83</accession>
    <name>A0A481QM83_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase</fullName>
            <ecNumber evidence="6 7">6.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">D-alanyl-D-alanine-adding enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 11" type="primary">murF</name>
        <name evidence="11" type="ORF">C3B55_00580</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine</text>
            <dbReference type="Rhea" id="RHEA:28374"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:83905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MurCDEF family. MurF subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.10" evidence="6 7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00580"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047480">
        <property type="term" value="F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008766">
        <property type="term" value="F:UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02019">
        <property type="entry name" value="MurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005863">
        <property type="entry name" value="UDP-N-AcMur_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01143">
        <property type="entry name" value="murF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 6 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 6 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="8">
        <location>
            <begin position="26"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="10">
        <location>
            <begin position="107"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="9">
        <location>
            <begin position="318"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="109"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="456" mass="48744" checksum="779A3080C66BF517" modified="2019-06-05" version="1">MLKAMRFNELTQTLSARILSNDCSFDGVSIDSRNIKPGQLFVALVGQSFDGHNYLNDVAAKGAAGALVQREVEDSSLAQLLVSDTRLAFGQLGALNRTAFKKPVTAITGSSGKTTVKELLASVLRTRGPVLATYGNLNNNFGVLLTLLELALEHTAAVIELGASRIGEIAYTVTLTKPHVIIINNAELAHVGEFGSPEKIVEAKGEIFEGLNASGTAVLNLDDKAFETWRIRAANRNVLTFAVLNTSADFCASNITIDARGCPSFTLHTPQGSEHVQLNLLGRHNVANALATAAAAYALGVSLFDTTIGLSTLQPIKGRTVAQLANNGMRIIDDTYNANQSSICAAIDLLNSFAGHKVLVLGDIAELGDWAEKSHREVGAYAIGKVDAFYAVGQNMVHAVNAFGRDAWHFATQAKLIQTLVTTEQEKQTTILIKGSRSAAMENVVAALCSFRQEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM84</accession>
    <name>A0A481QM84_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00339"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="39" mass="4719" checksum="B26A7E328DD0A4D6" modified="2019-06-05" version="1">MRSMPKNVTQYLLVQCIFEAYKRLEHRKNVPAKNVYYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMA4</accession>
    <name>A0A481QMA4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00619"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018721">
        <property type="entry name" value="DUF2252"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39441">
        <property type="entry name" value="PTHR39441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10009">
        <property type="entry name" value="DUF2252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="406" mass="46160" checksum="25AF871EF0D649E3" modified="2019-06-05" version="1">MEPFQYFVPSSSMKIPRPSNRMPHLIQLRNLKMASSAHAYVRGSTVQFYEWLHSQRGCRLPHGPEIWICGDCHAGNLGPTGDLRGRIDMYIRDLDQTVIGNPAHDLVRLALSLATVARGSDLPGVIIARMLEGIMLGYEYAFKDKQDKAALRPSQVKVAMHSSIEQTWKNLARKRIENTRPTIPLGKHFWSLSQAEKIALQTLCSSYEIHQLVNSFKGRHKDAKIKLLDSAYWVKGCSSLGLLRYAVLLGIGDKDDQRYCLIDIKEAVAATAPYIVKSNMPRDNSYRVVEGARHLSPGLGERMRATRFLDHVFFVRELLPQDMKLKLDELSETYAIYAAGYLASIIGIAHARQMDRDTRKDWMAVLQENRSKQLDAPSWLWTSVVQLVGNYEQGYLNHCRRYALKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMB8</accession>
    <name>A0A481QMB8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Threonine/homoserine exporter RhtA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rhtA</name>
        <name evidence="3" type="ORF">C3B55_00633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00633"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="157"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="303" mass="31970" checksum="949D3CA8E9A96BAC" modified="2019-06-05" version="1">MTSDEPTPMITPPRSLPSKLFPVGLLLIAMISIQSGASLAKSMFPVVGAQGTTTLRLIFASVIMLILLRPWRAKLTAKSLRTVVIYGIALGGMNFLFYMSLRTLPLSIAVALEFTGPLAVAIYALRKAVDFLWVSLAIIGLLLLIPTGKTDNTMDLVGVAYALGAGVCWALYILFGQKAGNDNGVQTAALGVMIAALFIAPIGIVHAGTALLTPALIPVAIGVAILSTALPYTLEMVALTRLPARTFGTLMSIEPAFGAFSGLFFLQEHLSIAQWLAIICIILASIGATLTMRNESKPLASAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMB9</accession>
    <name>A0A481QMB9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.117</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Tetrahydrodipicolinate N-succinyltransferase</fullName>
            <shortName evidence="4">THDP succinyltransferase</shortName>
            <shortName evidence="4">THP succinyltransferase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Tetrahydropicolinate succinylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">dapD</name>
        <name evidence="6" type="ORF">C3B55_00493</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the conversion of the cyclic tetrahydrodipicolinate (THDP) into the acyclic N-succinyl-L-2-amino-6-oxopimelate using succinyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + succinyl-CoA = (S)-2-succinylamino-6-oxoheptanedioate + CoA</text>
            <dbReference type="Rhea" id="RHEA:17325"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15685"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="2.3.1.117"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the type 2 tetrahydrodipicolinate N-succinyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.117" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00493"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00019"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008666">
        <property type="term" value="F:2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02122">
        <property type="entry name" value="DapD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019876">
        <property type="entry name" value="DapD_gammaproteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032784">
        <property type="entry name" value="THDPS_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038361">
        <property type="entry name" value="THDPS_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026586">
        <property type="entry name" value="Type2_DapD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14789">
        <property type="entry name" value="THDPS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03536">
        <property type="entry name" value="DapD_gpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="4" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THDPS_M" evidence="5">
        <location>
            <begin position="132"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Succinyl-CoA binding" evidence="4">
        <location>
            <begin position="279"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Succinyl-CoA binding" evidence="4">
        <location>
            <begin position="317"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-anhydride intermediate" evidence="4">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>ligand shared between trimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>succinyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>succinyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>succinyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>succinyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>succinyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>succinyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="344" mass="36434" checksum="7E54A74AF30392B6" modified="2019-06-05" version="1">MSNSLFSLGFGIGTQNREGAWLEVFYAQPLLNPSTKIIGAIAPILGYTEGNQAINFSASQSLQLTEALQSIDAAQATLLSRLAKSRTPLVATILAKDTALTSTPEAYLKLHLLSHRLVQPHGLSLADMFLQLPNVAWTNHGAINITELAERQLEARLCGELLEVFSVDKLPKMTDYVVPAGVRIADAARIRLGAYVGEGTTVMHEGFVNFNAGTEGPNMIEGRVSAGVFVHNGSDLGGGCSTMGTLSGGGNIVITVGEGCLIGANAGIGIPLGDRNTVESGLYITAGTKVALLDQQNELVKVVKARELAGQPDLLFRRNSETGVVECKTYKSAIELNKALHAHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMC3</accession>
    <name>A0A481QMC3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00624"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="39" mass="4489" checksum="7ABA41B9DD61A82F" modified="2019-06-05" version="1">MKALRKASLALEEGDLTLCMQVKYDEVIFEVICYSLYMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMF1</accession>
    <name>A0A481QMF1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00684</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00684"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021793">
        <property type="entry name" value="Oprl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11839">
        <property type="entry name" value="Alanine_zipper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019825837" evidence="2">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="83" mass="8949" checksum="6F7903521617F533" modified="2019-06-05" version="1">MNNVLKFSALALAVVLATSCSSVSKETETRLTTTEGTAVRSQARADEAYRKADDAMAAAQKAQQTADEANERALRMLEKASRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMF6</accession>
    <name>A0A481QMF6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00668"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="130" mass="14986" checksum="90E7EDA6309AD18E" modified="2019-06-05" version="1">MITFIRIIILGLVLWLSSCAIHQPSVPISQTIKWSNHLNQPRTESTHSVTPSPQPQPSSSPIFAPPLSPASHWDSKLQVYVLEEQPDTFYRQRTYYRWRNGWSCSISPNGPWKETNLYGVPQWLSKHYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMG9</accession>
    <name>A0A481QMG9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription-repair-coupling factor</fullName>
            <shortName evidence="8">TRCF</shortName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">mfd</name>
        <name evidence="11" type="ORF">C3B55_00713</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the C-terminal section; belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00713"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000716">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00969">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004576">
        <property type="entry name" value="Mfd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037235">
        <property type="entry name" value="TRCF-like_C_D7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005118">
        <property type="entry name" value="TRCF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03461">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00982">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143517">
        <property type="entry name" value="SSF143517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00580">
        <property type="entry name" value="mfd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="617"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="799"/>
            <end position="953"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="1149" mass="129214" checksum="6E6B8316B440A184" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMK5</accession>
    <name>A0A481QMK5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00729</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00729"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="50" mass="5977" checksum="C8FB2210B18567D7" modified="2019-06-05" version="1">MGKFYLLLKLIINMILLNYPTNVECLMFKSTIERKDNNNLYLNIFHLNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMK6</accession>
    <name>A0A481QMK6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">50S ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rplS</name>
        <name evidence="7" type="ORF">C3B55_00939</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00939"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00402">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="L19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_L19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018257">
        <property type="entry name" value="Ribosomal_L19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="PTHR15680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002191">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01024">
        <property type="entry name" value="rplS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01015">
        <property type="entry name" value="RIBOSOMAL_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="116" mass="13188" checksum="166412F252E03C94" modified="2019-06-05" version="1">MTNKIILALEAEQITKEMPVFAPGDTIVVQVKVKEGDRARLQAFEGIVIAKRNRGVNSAFTVRKISNGIGVERTFQTYSQQIDSITIKRRGDVRKAKLYYLRDLFGKAARIKEKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMK9</accession>
    <name>A0A481QMK9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase III subunit gamma/tau</fullName>
            <ecNumber evidence="5">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">dnaX</name>
        <name evidence="7" type="ORF">C3B55_00856</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DnaX/STICHEL family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00856"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18137">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022754">
        <property type="entry name" value="DNA_pol_III_gamma-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012763">
        <property type="entry name" value="DNA_pol_III_sug/sutau_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021029">
        <property type="entry name" value="DNA_pol_III_tau_dom-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045085">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038249">
        <property type="entry name" value="PolIII_tau_V_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12169">
        <property type="entry name" value="DNA_pol3_gamma3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12170">
        <property type="entry name" value="DNA_pol3_tau_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02397">
        <property type="entry name" value="dnaX_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="37"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="679" mass="75823" checksum="113E30EE5D1E74F4" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QML2</accession>
    <name>A0A481QML2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha</fullName>
            <shortName evidence="10">ACCase subunit alpha</shortName>
            <shortName evidence="10">Acetyl-CoA carboxylase carboxyltransferase subunit alpha</shortName>
            <ecNumber evidence="10">2.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">accA</name>
        <name evidence="12" type="ORF">C3B55_00756</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-CoA + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54728"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="EC" id="2.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the AccA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.15" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00756"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00823">
        <property type="entry name" value="AcetylCoA_CT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001095">
        <property type="entry name" value="Acetyl_CoA_COase_a_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42853">
        <property type="entry name" value="PTHR42853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03255">
        <property type="entry name" value="ACCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01069">
        <property type="entry name" value="ACCCTRFRASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00513">
        <property type="entry name" value="accA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="11">
        <location>
            <begin position="32"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="315" mass="34954" checksum="5546F7FEF9E5DE00" modified="2019-06-05" version="1">MNQNFLDFEQPIADLQAKIEELRLVGNDNLLNIDDEIARLQDKSNALTKDIFSRLTSWQIACLARHPCRPYTLDYIQYIFTEFNELHGDRHFSDDAAIVGGIARLDDQPVMVIGHQKGREVREKVRRNFGMPRPEGYRKACRLMKMAERFKMPILTFIDTPGAYPGIGAEERNQSEAIALNLRVMARLKVPIIATMIGEGGSGGALAIGVCDQLNMLQYSTYAVISPEGCASILWKTTEKASDAAEAMGVTADRLQGLGIVDKIITEPVGGAHRNPAAAAATIRTELGLQLTTLKKLSNEALLAHRYERLMHYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMM0</accession>
    <name>A0A481QMM0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cell division protein FtsB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">ftsB</name>
        <name evidence="4" type="ORF">C3B55_00751</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential cell division protein. May link together the upstream cell division proteins, which are predominantly cytoplasmic, with the downstream cell division proteins, which are predominantly periplasmic.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of a complex composed of FtsB, FtsL and FtsQ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <text evidence="3">Localizes to the division septum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FtsB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00751"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00599">
        <property type="entry name" value="FtsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023081">
        <property type="entry name" value="Cell_div_FtsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007060">
        <property type="entry name" value="FtsL/DivIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37485">
        <property type="entry name" value="PTHR37485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04977">
        <property type="entry name" value="DivIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="3">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic" evidence="3">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="92" mass="10722" checksum="D4E9BFFE144C5A9B" modified="2019-06-05" version="1">MRTPNWLFLVLFLLLINLQYRLWVGNGIYAQMKELTQQIASQHAENERLLESNRILDAEVLELKQGTETIEELAHHELGMVKNGETLYQLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMM4</accession>
    <name>A0A481QMM4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cdsA</name>
        <name evidence="9" type="ORF">C3B55_00766</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00766"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="269" mass="29129" checksum="50801CB85A74D014" modified="2019-06-05" version="1">MLKQRIITALIIIPIVWCGLFLLDGSIFALFIGLVITLGAWEWAGLAGLNACLLRVIYVAIVATLLFLMHVLSNIIVPWILCIAVLWWALAIFLVLTYPRTSNYWSSLATKLVIGLLILLPVSQGLAELKNAPLGNWLIMAVMVLVWSADIGAYFSGLAFGKRKLAPAVSPSKSWEGVYGGLALTLLITLIVGIMRDWSLKEILLALSIAVVAVFISIVGDLTESMFKRQAGIKDSSNLLPGHGGVLDRIDSLTAAIPIFAACLWMIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMM8</accession>
    <name>A0A481QMM8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyridoxine/pyridoxamine 5'-phosphate oxidase</fullName>
            <ecNumber evidence="5">1.4.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">PNP/PMP oxidase</fullName>
            <shortName evidence="5">PNPOx</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Pyridoxal 5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 10" type="primary">pdxH</name>
        <name evidence="10" type="ORF">C3B55_00743</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + O2 + pyridoxamine 5'-phosphate = H2O2 + NH4(+) + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15817"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O2 + pyridoxine 5'-phosphate = H2O2 + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15149"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 7">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="5 7">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the pyridoxamine 5'-phosphate oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00743"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00304"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine-phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01629">
        <property type="entry name" value="PdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019740">
        <property type="entry name" value="Pyridox_Oxase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019576">
        <property type="entry name" value="Pyridoxamine_oxidase_dimer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PTHR10851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10590">
        <property type="entry name" value="PNP_phzG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000190">
        <property type="entry name" value="Pyd_amn-ph_oxd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00558">
        <property type="entry name" value="pdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01064">
        <property type="entry name" value="PYRIDOX_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0288">FMN</keyword>
    <keyword evidence="4 5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="8">
        <location>
            <begin position="46"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="PNP_phzG_C" evidence="9">
        <location>
            <begin position="175"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="9"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5 6">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <begin position="64"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <begin position="79"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <begin position="143"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000190-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="215" mass="25002" checksum="ADDF24113BA5F677" modified="2019-06-05" version="1">MTKILSDMRRDYTRYSLSEAQASAEPFALFYQWFSDAMQTEQSPMEINAMTLSTVDHEGRPHCRILLLKGLDAHGFIFFTNYESAKGKQLAAWPFAAMTFFWPTLERQVRIEGLASKVTPEESDAYYEVRPLGSRLGAWASPQSEVVCDREELQGLLNATKQRFSHTQPDRPTYWGGYRLLPERIEFWQGRASRLHDRLNYRLKAGEWIRERLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMN1</accession>
    <name>A0A481QMN1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribonuclease G</fullName>
            <ecNumber evidence="7">3.1.26.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rng</name>
        <name evidence="7" type="ORF">C3B55_00530</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.1.26.-" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00530"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019307">
        <property type="entry name" value="RNA-bd_AU-1/RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004659">
        <property type="entry name" value="RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30001">
        <property type="entry name" value="PTHR30001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10150">
        <property type="entry name" value="RNase_E_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00757">
        <property type="entry name" value="RNaseEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="39"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="485" mass="55306" checksum="5DAD3E2A41958A6D" modified="2019-06-05" version="1">MSEEILINITPMESRVAVVENGILQEVHVERTRKRGIVGNIYKGKVVRVLPGMQAAFIDIGLDRAAFIHASEIFLCDGSAEASISVLVHEWQNIVVQVIRDEIGSKGARLTTQLSIPSRYLVYMPRTTHVGISLKIEAEAERERLKQIVSEYIAAENIKEMGGFILRTAAEGASATEILMDIRYLRRLWDQIDAQTKTISAPSVIYEDLCLALRALRDLVSPKIKKIRIDSRETFERATQFVAELMPEIADRLEHYHGERPIFDLYGVEDEIQKALEHKVLLKSGGYLVVDQAEAMTTIDVNTGTFVGYRNLEETIFKTNLEAATSISRQLRLRNLGGIIIIDFIDMEEKEHQRQVLRTLEKQMERDHAKTNIISITELGLVQMTRKRTRESLEQVLCEPCSSCHGRGKLKTPETVCYEIFHEILREARAYQAKGYKVLASQKVVDRLMDEESSNFAELENFIKRTISFQVETIYSQEQYDVVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMS1</accession>
    <name>A0A481QMS1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Heme exporter protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ccmB</name>
        <name evidence="14" type="ORF">C3B55_00675</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the CcmB/CycW/HelB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00675"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003544">
        <property type="entry name" value="Cyt_c_biogenesis_CcmB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026031">
        <property type="entry name" value="Cyt_c_CcmB_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30070">
        <property type="entry name" value="PTHR30070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03379">
        <property type="entry name" value="CcmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002764">
        <property type="entry name" value="CcmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01190">
        <property type="entry name" value="ccmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="11 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR002764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="217" mass="22996" checksum="9DC569BC64544A11" modified="2019-06-05" version="1">MLFTHEVRLLCRRPAELANPLIFFTIVIALFSLAIGPETNLLQTLSPGLVWVAALLSVLLSLDELFRSDFEDGSLEQWVLSSQPLPLLVLTKVLANCSFSGLMLIILSPLLATMLGLPAECLSILLLSLLLGIPVLSLLGAVGAVLTVGLKRGGLLLALLILPLYIPVLIFGSGALWAALIGMPATGYLLWLGSLTAMAVTMTPFAIAAGLKIRVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMT4</accession>
    <name>A0A481QMT4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="13">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="13">2.7.8.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pgsA</name>
        <name evidence="13" type="ORF">C3B55_00592</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="13"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00592"/>
    <dbReference type="UniPathway" id="UPA00185">
        <property type="reaction ID" value="UER00282"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00560">
        <property type="entry name" value="pgsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="189" mass="21291" checksum="D59D01A5CA19A868" modified="2019-06-05" version="1">MNIPNLITILRVLFIPIFILLFYMPYEWSYAASSSVFVFAATTDWLDGYMARRLEQSTPFGAFLDPVADKVMVAISLVLLVQEHSNFWLTLPAAVIIGREIVVSALREWMAEIGARAHVAVSNMGKWKTAAQMLALVILLANPPDFSLYVLTGYALLLIAAGLTLWSMLQYLRIALPYMRTNVEQNKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMX5</accession>
    <name>A0A481QMX5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00848"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="61" mass="6823" checksum="DDC0EDD0B9831FB8" modified="2019-06-05" version="1">MLPNISGKIFPSSFDCFSTVSRTKIALRSIAVFAYDEFNSTTGRFTAGKIFYRLNTIYGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN06</accession>
    <name>A0A481QN06_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Electron transfer flavoprotein-ubiquinone oxidoreductase</fullName>
            <shortName evidence="9">ETF-QO</shortName>
            <ecNumber evidence="9">1.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">C3B55_00871</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Accepts electrons from ETF and reduces ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a ubiquinone + reduced [electron-transfer flavoprotein] = a ubiquinol + H(+) + oxidized [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24052"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.5.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.1" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00871"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040156">
        <property type="entry name" value="ETF-QO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617">
        <property type="entry name" value="PTHR10617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="8 9 10" id="KW-0830">Ubiquinone</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="554" mass="61012" checksum="6F5E210BFF762E2C" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN21</accession>
    <name>A0A481QN21_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease E</fullName>
            <shortName evidence="5">RNase E</shortName>
            <ecNumber evidence="5">3.1.26.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">rne</name>
        <name evidence="8" type="ORF">C3B55_00884</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.</text>
            <dbReference type="EC" id="3.1.26.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions per homotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNase E/G family. RNase E subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.12" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00884"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008995">
        <property type="term" value="F:ribonuclease E activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00970">
        <property type="entry name" value="RNase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019307">
        <property type="entry name" value="RNA-bd_AU-1/RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028878">
        <property type="entry name" value="RNase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004659">
        <property type="entry name" value="RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30001">
        <property type="entry name" value="PTHR30001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10150">
        <property type="entry name" value="RNase_E_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00757">
        <property type="entry name" value="RNaseEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="55"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Required for zinc-mediated homotetramerization and catalytic activity" evidence="5">
        <location>
            <begin position="417"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="608"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="673"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="838"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="618"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="673"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="706"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="895" mass="100688" checksum="BC4F67FDE6266EEC" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN55</accession>
    <name>A0A481QN55_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Translation initiation factor IF-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">infC</name>
        <name evidence="8" type="ORF">C3B55_00791</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the IF-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00791"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00080">
        <property type="entry name" value="IF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036787">
        <property type="entry name" value="T_IF-3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019813">
        <property type="entry name" value="Translation_initiation_fac3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001288">
        <property type="entry name" value="Translation_initiation_fac_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019815">
        <property type="entry name" value="Translation_initiation_fac_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019814">
        <property type="entry name" value="Translation_initiation_fac_3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938">
        <property type="entry name" value="PTHR10938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00707">
        <property type="entry name" value="IF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05198">
        <property type="entry name" value="IF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54364">
        <property type="entry name" value="SSF54364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55200">
        <property type="entry name" value="SSF55200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00168">
        <property type="entry name" value="infC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00938">
        <property type="entry name" value="IF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF3_N" evidence="7">
        <location>
            <begin position="13"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="IF3_C" evidence="6">
        <location>
            <begin position="89"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="178" mass="20267" checksum="337E55E2DB8E96FA" modified="2019-06-05" version="1">MRQDKRAAPKAPINENISAFEVRLIGANGEQIGIVLIDEALRIAEESKLDLVEISANAVPPVCRVMDYGKSIFEKKKQIAAAKKNQKQIQVKEIKFRPGTDEWDYQVKLRNLVRFLKDGDRAKVSLRFRGREMAHQELGIELLKRVALDLLEYGSVEQLPKMEGRQLIMVIAPKKRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN78</accession>
    <name>A0A481QN78_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00722</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00722"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="66" mass="7153" checksum="53A35F0ABB2F7D0B" modified="2019-06-05" version="1">MVPLLSSRVLKVRSTNNDIITANSLLYKVAASVVDLFNGDALAYSKIAGITRSSILIVIQLICLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN84</accession>
    <name>A0A481QN84_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arginine exporter protein ArgO</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">argO</name>
        <name evidence="6" type="ORF">C3B55_00903</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00903"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="199" mass="21597" checksum="EF49AE328D4A0EC6" modified="2019-06-05" version="1">MWQSYVNGLLMAFGLIMAIGTQNTFVLTQSLRREHHLPVAALCIVCDAILVAAGVFGLAAILIQNPLLLAIARWGGAAFLLWYGALALRRAFSKQSLKEVVQLKARSLRTVLLSALAVTLLNPHVYLDTVLLIGSLGAQQTEPGAYVAGAASASFIWFTTLALGASWLAPWLKRPATWRLLDFLVVVMTFSVAYQLINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN87</accession>
    <name>A0A481QN87_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00949</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00949"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="49" mass="5343" checksum="0EA608DCA9817699" modified="2019-06-05" version="1">MCLGIGHHTSIIALQIFLVNSNLMAMKVSGEYLKVYLVSGCQAAYLINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNC1</accession>
    <name>A0A481QNC1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA(Ile)-lysidine synthase</fullName>
            <ecNumber evidence="7">6.3.4.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA(Ile)-2-lysyl-cytidine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">tRNA(Ile)-lysidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">tilS</name>
        <name evidence="10" type="ORF">C3B55_00755</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the tRNA(Ile)-lysidine synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00755"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="PP-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012796">
        <property type="entry name" value="Lysidine-tRNA-synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015262">
        <property type="entry name" value="tRNA_Ile_lys_synt_subst-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="PTHR43033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09179">
        <property type="entry name" value="TilS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11734">
        <property type="entry name" value="TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00977">
        <property type="entry name" value="TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02433">
        <property type="entry name" value="lysidine_TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="TilS_C" evidence="9">
        <location>
            <begin position="362"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="27"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="440" mass="49405" checksum="DAA1DFE205AD39DA" modified="2019-06-05" version="1">MNSTIISKALQALTPWRYAKVWHIAFSGGLDSTVLLYFLATMAKTETLPKLNVIHVNHGLQTAADAWLSHCQTVCNSLCVPLQVIHVQVQAGASLECAARNARYKAFIEVTGVGEVLITGQHCNDQAETLLLQLLRGAGVRGLSAMPMYRQLAGGHLVRPFLSISRAQLEAYANEYRLQWIEDNSNADTKFSRNYLRHCIFPLLIERWPQAIASLARAAKHIGEAQYLLDELAVIDQQTASQPSPFPWLTLPSLALAPLRKLSDTRQRNALRHWLALLTRLPDSAHWAGWYSLRDAKRDAQPTWRLTDGQLCRCGERIWWLPSASYQKFFDAPVSWSQPKKPLKLPGNGQLKLIGTIPTGPLVVRYRQGGEIIEVPGRGLRNLKRLLNESCLPSFIRRRLPLLYRGEELLAVPAIAGLWVSGPGDWQLHWIPQIYDQGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNE6</accession>
    <name>A0A481QNE6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="9">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">guaA</name>
        <name evidence="12" type="ORF">C3B55_00946</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00946"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 9 10" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="3 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="11">
        <location>
            <begin position="208"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="235"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="525" mass="59049" checksum="BE3D4438951C99A7" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNG9</accession>
    <name>A0A481QNG9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc import ATP-binding protein ZnuC</fullName>
            <ecNumber evidence="8">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">znuC</name>
        <name evidence="8" type="ORF">C3B55_00997</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.-" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00997"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015633">
        <property type="term" value="F:ABC-type zinc transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017882">
        <property type="entry name" value="ZnuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734:SF9">
        <property type="entry name" value="PTHR42734:SF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0864">Zinc transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="6"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="249" mass="27623" checksum="BBC17BAE6F0375B9" modified="2019-06-05" version="1">MSNALIRLQDISVMFARQNVLDNITISVEPGQIVSLIGPNGAGKTTLVRTVLGLIKPDNGNVWRKQKLRIGYMPQKLHIDPTLPLSVLRFLCLMPGVDRISTQAALKEVGAEQVINSPVQNISDGEMQRIFLARALLRKPELLVLDEPMQGIDVAGQAALYSLITKLRDRYECGVLMVSHDLHLVMKTTDMVVCLNHHVCCSGHPKQVSGDQAFIELFGKNAQTLAIYHHHHDHDHDLHGTIIDDPTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNH0</accession>
    <name>A0A481QNH0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="9">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glycyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="9">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">glyQ</name>
        <name evidence="10" type="ORF">C3B55_01012</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01012"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00733">
        <property type="entry name" value="GlyRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00254">
        <property type="entry name" value="Gly_tRNA_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002310">
        <property type="entry name" value="Gly-tRNA_ligase_asu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02091">
        <property type="entry name" value="tRNA-synt_2e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01044">
        <property type="entry name" value="TRNASYNTHGA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00388">
        <property type="entry name" value="glyQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="317" mass="36539" checksum="3D0766960669422B" modified="2019-06-05" version="1">MSQPTPTLYTFQYLLLALQQYWADQGCIVMQPYDMEVGAGTFHTATFLRAIGPETWNVVYIQPSRRPTDGRYGKNPNRLQHYYQLQVVMKPNPNNFQELYLDSLKHVGLDPLVHDIRFAEDNWESPTLGAWGLGWEVWLNGMEITQFTYFQQAGGIDCYPVVGELTYGLERLAMYLQDVNSVYDLVWADGPFGKVTYGDLFHQNETEQSIYNFEIANVEKLFELFDLYESEAKRLIKLDQPLPLPGYEMVLQASHIFNLLDARHAISVTSRQQYMLRIRTLAYSIAQAYLLARAKLGFPMAPQDLRDIVLPALEVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNI0</accession>
    <name>A0A481QNI0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">DNA gyrase subunit B</fullName>
            <ecNumber evidence="4 12">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">gyrB</name>
        <name evidence="14" type="ORF">C3B55_01015</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="12">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase GyrB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4 12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01015"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03366">
        <property type="entry name" value="TOPRIM_TopoIIA_GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01898">
        <property type="entry name" value="GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002288">
        <property type="entry name" value="DNA_gyrase_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011557">
        <property type="entry name" value="GyrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041423">
        <property type="entry name" value="GyrB_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034160">
        <property type="entry name" value="TOPRIM_GyrB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00986">
        <property type="entry name" value="DNA_gyraseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18053">
        <property type="entry name" value="GyrB_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01059">
        <property type="entry name" value="gyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="13">
        <location>
            <begin position="419"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX82313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="805" mass="90837" checksum="8E15286A7796CA6C" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNI6</accession>
    <name>A0A481QNI6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00979</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00979"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="47" mass="5293" checksum="72834022F581406C" modified="2019-06-05" version="1">MTSILLMLEDAFMIKHIVYLICCLRNGGMHLIISHTSAFTATPRMVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNJ8</accession>
    <name>A0A481QNJ8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00989"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="38" mass="4084" checksum="5B1B982B81E43AA9" modified="2019-06-05" version="1">MIARGQGEHAVIVARFTKYNVECLLTADTKCVSSSNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNT9</accession>
    <name>A0A481QNT9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00854</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00854"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="45" mass="5158" checksum="A16EB3BC9CA5CBFC" modified="2019-06-05" version="1">MISIAFKNCTQKQFEALYIVILKYNPDNNSCSAKNVLRADICNVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QP19</accession>
    <name>A0A481QP19_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="5">CDG synthase</shortName>
            <ecNumber evidence="5">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Queuosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">queE</name>
        <name evidence="7" type="ORF">C3B55_00911</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-deazaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="5">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00911"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027621">
        <property type="entry name" value="rSAM_QueE_gams"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000370">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04349">
        <property type="entry name" value="rSAM_QueE_gams"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="25"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="220" mass="24773" checksum="4E0BE14E3FB59A1A" modified="2019-06-05" version="1">MPVATMQNTLRITEIFYSLQGETRTAGLPTVFIRLTGCPLRCQYCDSAYAFSGGTVYTIDNIIEQVAYFKPRYACVTGGEPLAQPNSIQLLKRLCDTGYEVSLETSGALDISAVDLRVSRVVDLKTPDSKEAHRNLYDNIDLLTANDQVKFVICSRGDYNWATSKLIQYDLNRRAGEVLFSLSHDDLNARDLADWVVADNLPVRLQLQLHKYLWNNDPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QPF1</accession>
    <name>A0A481QPF1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Octaprenyl-diphosphate synthase</fullName>
            <ecNumber evidence="3">2.5.1.90</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ispB</name>
        <name evidence="3" type="ORF">C3B55_00985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.90" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00985"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106350">
        <property type="term" value="F:octaprenyl pyrophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="322" mass="34958" checksum="D0045B3028A26ED1" modified="2019-06-05" version="1">MQPQAFYRTVADDFSAVDSIIKQQLTSKVPLVSKIGDYITSAGGKHLRPLLVLLCGKALGREGGDLRLLAATIEFLHTATLLHDDVVDMSGMRRGRETAHAMWGVAPSILVGDFLYSRSFEMMVELGSIPVMKILSQATRIIAEGEVLQLSKIRDVSTTEETYMNVIRGKTAMLFEAATHSAAALCGASAEKAEALRTFGDYLGIAFQLVDDLLDYQGDTEALGKNIGDDLAEGKPTLPLIYTMREGTPEQAALIRKAIQQGGIEDLSAIRTAIEASGSLVYTAQLARNYVARAIQCLETLPTSEYRDALVELSEFAVARTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QJX4</accession>
    <name>A0A481QJX4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA modification GTPase MnmE</fullName>
            <ecNumber evidence="6">3.6.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">mnmE</name>
        <name evidence="6" type="synonym">trmE</name>
        <name evidence="9" type="ORF">C3B55_00001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="6">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00001"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51709">
        <property type="entry name" value="G_TRME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TrmE-type G" evidence="8">
        <location>
            <begin position="216"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="226"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="245"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="270"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="456" mass="49623" checksum="417E6B96FADB209F" modified="2019-06-05" version="1">MMALSETIAAVATNQGRGGIGIVRISGSLAGSVSQAITGRKLKPRFAHYGPFLDASKCVLDEGLAIYFPRPNSFTGEDVLELHGHGSPVVLNMILQRCLQLGCRLARPGEFSERAFLNDKLDLAQAEAIADLIESSSEQAARNALRSLQGLFSIRIHNLIEQLINLRLYVEAAIDFPEEEIDFLADDHVLACLNKVRDELSTVQHEAGQGALMRDGMTVVIAGRPNAGKSSLFNALTGREAAIVTEIAGTTRDIIQEHIHIDGMPIRVVDTAGLHDTANQVEKIGVDRALQAIDEADHVLLVVDATTPEAVNPFNLWPEFFKQQPDLHKVTVIRNKTDLTNESIAIKTSKDGHVTISLSIKSTNDGLELLRNHLKACVGYTQTPENSFSARQRHLKALHDASIKLEHASVQLNLTGAGELLAEDLRQAQQILSEITGVFSSDDLLGKIFSNFCVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK62</accession>
    <name>A0A481QK62_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">trmB</name>
        <name evidence="2" type="ORF">C3B55_00081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00081"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01057">
        <property type="entry name" value="tRNA_methyltr_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="PTHR23417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00091">
        <property type="entry name" value="TIGR00091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="219"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="241" mass="27575" checksum="29F8B626C23824D3" modified="2019-06-05" version="1">MIKSNEILYTVKKGNEARYRRIKSFVMRAGRMTKGQQNGLEQGTPLFVLPLANSPVDYDQIFGRLAQRSLEIGFGMGQSLLKMAASAPEQDFIGVEVYRPGVGALLNGILTQGLTNLRVYNCDAIEVLNRCIVDNSLDHLMLFFPDPWHKSRHYKRRIVQASFAELVRTKLKVNGILHMATDWKPYAEYMLKVMDIAPGYHNLAESGRCVPRPVDRPITRFECRGERLGHKIWDFKFEKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKF2</accession>
    <name>A0A481QKF2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">DNA primase</fullName>
            <ecNumber evidence="12">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 16" type="primary">dnaG</name>
        <name evidence="16" type="ORF">C3B55_00111</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 13 14">Binds 1 zinc ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer. Interacts with DnaB.</text>
    </comment>
    <comment type="domain">
        <text evidence="12">Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00111"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03364">
        <property type="entry name" value="TOPRIM_DnaG_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00974">
        <property type="entry name" value="DNA_primase_DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016136">
        <property type="entry name" value="DNA_helicase_N/primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013264">
        <property type="entry name" value="DNA_primase_core_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037068">
        <property type="entry name" value="DNA_primase_core_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019475">
        <property type="entry name" value="DNA_primase_DnaB-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006295">
        <property type="entry name" value="DNA_primase_DnaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013173">
        <property type="entry name" value="DNA_primase_DnaG_DnaB-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030846">
        <property type="entry name" value="DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034151">
        <property type="entry name" value="TOPRIM_DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002694">
        <property type="entry name" value="Znf_CHC2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10410">
        <property type="entry name" value="DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08278">
        <property type="entry name" value="DnaG_DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08275">
        <property type="entry name" value="Toprim_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01807">
        <property type="entry name" value="zf-CHC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002811">
        <property type="entry name" value="DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00766">
        <property type="entry name" value="DnaG_DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00400">
        <property type="entry name" value="ZnF_CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01391">
        <property type="entry name" value="dnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 12 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 12 13" id="KW-0639">Primosome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 12 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="15">
        <location>
            <begin position="261"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CHC2-type" evidence="12 14">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR002811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR002811-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX81481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="656" mass="73964" checksum="C235E9A13A96F819" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKI6</accession>
    <name>A0A481QKI6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putrescine-binding periplasmic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">potF</name>
        <name evidence="1" type="ORF">C3B55_00059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00059"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019808">
        <property type="term" value="F:polyamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015846">
        <property type="term" value="P:polyamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001188">
        <property type="entry name" value="Sperm_putr-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019574">
        <property type="entry name" value="Periplasmic_polyamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00909">
        <property type="entry name" value="SPERMDNBNDNG"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="370" mass="40824" checksum="2CB142377B2C100D" modified="2019-06-05" version="1">MKALCLKDAGRKTFLVLYIIGAITNTVYANDKVLHVYNWSDYIAQDTISNFEKESGVKVVYDVFDSNETLEAKLLAGKSGYDIVVPSNNFLAKQIKAGVYQELDKSKLLDYDNLNKSLLKAVSISDPDNRHAFPYMWGSIGIGYNPEKVKAVLGIDKIDSWSTILKPENLAKQKNCGVSFLDSPTEMLPVVLHYLGLPTDTQKKADLKQAEDLFLKLRPSIRYFHSSKYISDLANGNICVAVGYSGDVQQAKSRAVEAGGKIKVAYDIPREGAGSFYDMIAVPKDAENIGAAYKFMNYLFKPEVMAAITNSVHFPNGNEKATALVDKDITSDPGSYPPADVQAKLYAIADLPAATQREMTRSWTKIKSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKK9</accession>
    <name>A0A481QKK9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00020"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="74" mass="8418" checksum="5BC4F8F08FCE07DC" modified="2019-06-05" version="1">MSVYILVSMQYWLGRASYEVRNGSPSNHCIGTANTAYLVKILLEWLRINFVAIIYPLTGSQNNDVNHNNIMTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKM7</accession>
    <name>A0A481QKM7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName evidence="8">PRA-PH</shortName>
            <ecNumber evidence="8">3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">hisE</name>
        <name evidence="9" type="ORF">C3B55_00175</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PRA-PH family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00175"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="110" mass="12292" checksum="71D6A887C36DCF0F" modified="2019-06-05" version="1">MSDTLNRIAQVLEDRKGTNADNSYVTSLYHKGLNKILEKLGEESIETIIAAKDAQISGDHNDVIYETADLWFHSLVMLAQLGQHPQAVLDELDRRFGLSGYTEKASRTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKN2</accession>
    <name>A0A481QKN2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Amino-acid carrier protein AlsT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">alsT</name>
        <name evidence="9" type="ORF">C3B55_00022</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00022"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015655">
        <property type="term" value="F:alanine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001463">
        <property type="entry name" value="Na/Ala_symport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30330">
        <property type="entry name" value="PTHR30330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01235">
        <property type="entry name" value="Na_Ala_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00175">
        <property type="entry name" value="NAALASMPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00835">
        <property type="entry name" value="agcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00873">
        <property type="entry name" value="NA_ALANINE_SYMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="479" mass="51950" checksum="48E1B4F123B08075" modified="2019-06-05" version="1">MLEVINNFLSGKVLIVLIIGLGSYFTICSRFVQLRHFFHMFSVFRRSLKSSAGQITSFQALMLSLAGRVGAGNISGVGIAVTLGGPGAVFWMWVTALIGMSSSFIECSLGQLYKRTDAKGAYHGGPAYYIQHGLQKRWLGMVMAFLLLVTFGFAFNGLQAHAVTHSLNNAFSLDTTYTGFTLAVLLGLVFIGGLKRIAWIADLLVPLKTVAYISVTIYVIVLQFDHVPTMMATIVKSAFGLDQAFSGLVGSTIIIGVKRGVFANEAGLGSVPNVAAVASVQHPINQGLIQAFSVFLDTFVICTCTALLILLSGFYTPGFEGDGIALTQNSLAAVVGEWGRMFVSVALALFVFTSIIYNYYLGESNLCFLVGNNHKILISYRGIVLALIFWGSIENLSTVFAFADITMTIVAFVNLFALAFLFKIAVRILNDYDNQRAAGIKTPMFDSSQFPDLDLDRNAWPAYTVKPDVTASLQLHAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKS0</accession>
    <name>A0A481QKS0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="3">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">C3B55_00052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = 5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00052"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="PTHR23407:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="9"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="140"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="204" mass="23664" checksum="7559AA13DAC1D0E1" modified="2019-06-05" version="1">MTEPKLLSRSQLRRILRKTRNALTPSEQRQAAQGLYRQMAQHPLFRRVKHISLYISTDGEINPHLLLRAAQRRGKATYLPVLSTWPRTKIVFQRVRPEDKLLTNRFNILEPQMHANCQRCIWALDLVLLPLVGFDNAGGRLGMGGGFYDRKLAYLARRKNWHKPTLLGLAHECQKVDFLVRASWDVKLAGIVTNKKWYIVKNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKS2</accession>
    <name>A0A481QKS2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="9">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">argS</name>
        <name evidence="13" type="ORF">C3B55_00181</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00181"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arg_tRNA_synt_N" evidence="12">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="DALR_1" evidence="11">
        <location>
            <begin position="462"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="578" mass="64712" checksum="3A93216469EF261E" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKT8</accession>
    <name>A0A481QKT8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Glutamine synthetase</fullName>
            <ecNumber evidence="2 7">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glnA</name>
        <name evidence="9" type="ORF">C3B55_00168</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Oligomer of 12 subunits arranged in the form of two hexagons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="2 7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00168"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001637">
        <property type="entry name" value="Gln_synth_I_adenylation_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00182">
        <property type="entry name" value="GLNA_ADENYLATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="8">
        <location>
            <begin position="101"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="4">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="468" mass="51750" checksum="CB2E30017116F92C" modified="2019-06-05" version="1">MSKSVRLIKDHNVKWIDLHFTDTKGSQHHVTMPARDALDNALLKEGKMFDGSSIAGWKGIEDSDMILMPDDSTAVIDPFTEDPTLIIICDVIEPSTMQGYDRDPRAVAKRAEAYLKLTGIGDTILVGPEPEFFIFNSVKFKSDTSGSMFKIFSEQGSWMSDQDIEGGNKGHRPGIKGGYCPVSPFDHDYEVRASMCNAMEEMGLIIEVHHHEVATAGQNEIGVKFNTLVAKADEVQTLKYCVHNVANAYGRTATFMPKPLYGDNGSGMHVHLSIAKEGKNTFTGKGYADLSDTALYFIGGIIQHGKALNGFTNPSTNSYKRLIPGFEAPVMLAYSACNRSASIRIPYASNPHAHRIEARFPDPSANPYLAFAALIMAGLDGIQNKVHPGDAIDKNLYDLLPEEVKEIPQVCSSLKEALEELNKDRAFLTKGDVFSDDFINAYIELKSEEEVKVRTCVHPLEYELYYSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-08-12" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKV6</accession>
    <name>A0A481QKV6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enamine/imine deaminase</fullName>
            <ecNumber evidence="2">3.5.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">yabJ</name>
        <name evidence="2" type="ORF">C3B55_00025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00025"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="PTHR11803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00004">
        <property type="entry name" value="TIGR00004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="126" mass="13710" checksum="94366DE4E8745FDE" modified="2019-06-05" version="1">MTKTAITSDKAPNAIGAYSQAIRSGNTVYMSSQIPLSPKSIKLAEGFEAQAVQIFENLKSVAEAASSSLKDIVKLNIFLTDLNNLTKLNEIMGRYFKQPYPARTTISVNSLQENAQVVVDAILTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKV8</accession>
    <name>A0A481QKV8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">rplA</name>
        <name evidence="10" type="ORF">C3B55_00267</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00267"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_B">
        <property type="entry name" value="Ribosomal_L1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005878">
        <property type="entry name" value="Ribosom_L1_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_L1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01169">
        <property type="entry name" value="rplA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="8" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="231" mass="24425" checksum="CE88D45A2AE4C8E9" modified="2019-06-05" version="1">MAKLTKRQKAIAGKIEVDQAYDFISATKLLTELSTLKFIESVDVAINLGVDPRKSDQIIRSSTVLPHGTGKIVRVAVFTQGPAASAAIVAGADRVGMDDLAAEIKKGDLNYDVVIASPDAMRVVGQLGQILGPRGLMPNPKVGTLTPGIATAVKNAKAGQVRYRTDKNGIIHTSVGNIGFDAIKLKENVKALISDLKRIRPVSGKGIYIKRVTLSTTMGPGLVIDQGSLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKW4</accession>
    <name>A0A481QKW4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis accessory factor UbiK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiK</name>
        <name evidence="2" type="ORF">C3B55_00035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for efficient ubiquinone (coenzyme Q) biosynthesis. UbiK is probably an accessory factor of Ubi enzymes and facilitates ubiquinone biosynthesis by acting as an assembly factor, a targeting factor, or both.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiK family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00035"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02216">
        <property type="entry name" value="UbiK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007475">
        <property type="entry name" value="UbiK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38040">
        <property type="entry name" value="PTHR38040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04380">
        <property type="entry name" value="BMFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="92" mass="10488" checksum="67F41D374FE72D03" modified="2019-06-05" version="1">MFVPKDFLDNLSVHAARLFNGDTPLLRNEIESQLKTLLQSSFNKLDLVSRKEFDSQMIVLTRTCERLESLETKVAALETRLAPLCADHTHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKW8</accession>
    <name>A0A481QKW8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Biotin synthase</fullName>
            <ecNumber evidence="3 13">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 16" type="primary">bioB</name>
        <name evidence="16" type="ORF">C3B55_00106</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>(4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:22060"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="EC" id="2.8.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="13 14">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13 14">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="3 13"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00106"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00272">
        <property type="entry name" value="biotin_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13 14" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 13 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 13" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 13 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="15">
        <location>
            <begin position="44"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001619-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX81477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="351" mass="39006" checksum="E95813EE6DF6D9D2" modified="2019-06-05" version="1">MSASTPATLRHDWSLAEVRALFLQPFNDLLFQAQTVHRTHFDANRVQVSTLLSIKTGACPEDCKYCPQSGHYNSGLEKEKLMEVHKVLEEAVRAKAIGSTRFCMGAAWKHPSAKDMPYILQMVKGVKAMGLETCMTIGRLDWDQAKALAQAGLDYYNHNLDTSPQFYGSIITTRTYSERLQTLAYVRDAGMKICSGGILGMGESLDDRANLLIQLANLPEHPESVPINMLVKVAGTPLGNAKDTDPFDFIRMLAVTRILMQQSHVRLSAGREAMNEQMQALAFFAGANSIFYGDKLLTTTNPQINQDIQLFARLGILPEEREEHPDEVHQAAIEQALVEQKSSALFYSAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKX2</accession>
    <name>A0A481QKX2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipopolysaccharide core heptose(I) kinase</fullName>
            <ecNumber evidence="1">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rfaP</name>
        <name evidence="3" type="ORF">C3B55_00200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; LPS core biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. KdkA/rfaP family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00200"/>
    <dbReference type="UniPathway" id="UPA00958"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009244">
        <property type="term" value="P:lipopolysaccharide core region biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017172">
        <property type="entry name" value="Lsacc_core_hep_kinase_RfaP"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037318">
        <property type="entry name" value="RfaP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR037318-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="268" mass="30874" checksum="96AEF8DD328BFE93" modified="2019-06-05" version="1">MKLILAEPFKTLWSGRDVFAEVEKLQGQVFRELTVRRTLRTEVDGRQYFVKIHRSIGWGEIFKNLITAKLPVLGVGLEWAAIHRLQKLGVPTMTGVAFSEKGSNVTNRYSFIITEELAPAISLENLTVNWVAKPPAFALRYAITAELARIVGKMHRGGVNHRDCYLCHFLLDTTKPMNVRKLKLSVIDLHRAQLRTHLPLRWRNKDLSALYYSALEIGLTQRDKWRFLKGYFRQPLRQILTEQSTSLGLMQRKAYKLYARKLSLGNVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKY0</accession>
    <name>A0A481QKY0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">rplN</name>
        <name evidence="6" type="ORF">C3B55_00287</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 5">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00287"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_L14P_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="116" mass="12818" checksum="F9732066EF833D75" modified="2019-06-05" version="1">MLDVADNSGARRIMCIKVLGGSHRRYACVGNIIKVTIKEAIPRGKVKKGQVMTAIVVRTRHGVRRADGSIIRFDGNAAVLLNNKQEPIGTRIFGPVPRELRTERFMKIVSLAPEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL01</accession>
    <name>A0A481QL01_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00058"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="86" mass="10199" checksum="51B86407DFE84B95" modified="2019-06-05" version="1">MSVRLIFNISVKQHSQKAYISNNLDQLINYLIDYKIIKVKYIIGWLISITYLVKVSLTNKLVAGKGWKRHWPTRDLFIVDLNMQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL16</accession>
    <name>A0A481QL16_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="8">2.2.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">1-deoxyxylulose-5-phosphate synthase</fullName>
            <shortName evidence="8">DXP synthase</shortName>
            <shortName evidence="8">DXPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">dxs</name>
        <name evidence="10" type="ORF">C3B55_00316</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D-xylulose 5-phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:12605"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00316"/>
    <dbReference type="UniPathway" id="UPA00064">
        <property type="reaction ID" value="UER00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052865">
        <property type="term" value="P:1-deoxy-D-xylulose 5-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="PTHR43322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00204">
        <property type="entry name" value="dxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transket_pyr" evidence="9">
        <location>
            <begin position="327"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine pyrophosphate binding" evidence="8">
        <location>
            <begin position="128"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine pyrophosphate binding" evidence="8">
        <location>
            <begin position="160"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="637" mass="69572" checksum="62727924EDC5611F" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL18</accession>
    <name>A0A481QL18_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Toxin RTX-I translocation ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">apxIB</name>
        <name evidence="11" type="ORF">C3B55_00136</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00136"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017750">
        <property type="entry name" value="ATPase_T1SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005074">
        <property type="entry name" value="Peptidase_C39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03375">
        <property type="entry name" value="type_I_sec_LssB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50990">
        <property type="entry name" value="PEPTIDASE_C39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C39" evidence="10">
        <location>
            <begin position="9"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="172"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="484"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="518"/>
            <end position="525"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="718" mass="78885" checksum="9914E89E914E6A96" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL54</accession>
    <name>A0A481QL54_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Type III pantothenate kinase</fullName>
            <ecNumber evidence="6">2.7.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">PanK-III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pantothenic acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">coaX</name>
        <name evidence="7" type="ORF">C3B55_00259</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>NH4(+)</name>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </cofactor>
        <cofactor evidence="6">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="6">A monovalent cation. Ammonium or potassium.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the type III pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00259"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00352"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01274">
        <property type="entry name" value="Pantothen_kinase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004619">
        <property type="entry name" value="Type_III_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34265">
        <property type="entry name" value="PTHR34265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03309">
        <property type="entry name" value="Pan_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00671">
        <property type="entry name" value="baf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="100"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="6"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="249" mass="27228" checksum="9C94754629FD135E" modified="2019-06-05" version="1">MILELDCGNSFIKWRILDLYTDKISLEGVVSSNLELIENLVAIRWLSLVSCRLVSVRTSEETRKLIAILQETFGVTVSCAAPAQEMAGVRNGYKDFECLGLDRWLAILGGFKLASGACLVFDFGTAATADFIAEDGEHLGGFICPGVSLMRSQLRTYTRKIRYNDVAAKQVTACISPGRTTVEAVERGCTLMLRGFIVIQLELARSQWGDDFTVFLTGRDAYLASDAVPCARLVPDLVFVGLAIACPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-06-17" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL67</accession>
    <name>A0A481QL67_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00380"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="33" mass="3645" checksum="D13BA5DF88168DF8" modified="2019-06-05" version="1">MQPATAEDNNIVILLATYLGTRHLIDNLHIGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL68</accession>
    <name>A0A481QL68_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00214"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="8"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="390" mass="42097" checksum="59208A3F1D24D744" modified="2019-06-05" version="1">MLPIISTDILIVGAGVAGLWLNARLRRQGFSTVLVESATLGGWQSINSQGIIHGGVKYALRGVFTGASEAIADMPRRWREALAGDGELDLHGVRLLSEANYLWSPSTTAGNLTSFLASKAMRGRVDQVNGDNLPSALQSSHFNGKVYRLAEIVVDVPSLVKRLAALAGDGLLAGQHIEPLLRGDDTLVGLKVDGRDIHAQRIVLSAGNGITNLLAALGMNQPIMQKRPLHMVIAKGPGLTPLYMHCLSNSTKPHLTITTHSTINGNLVWYIGGEIAEADGVRRTKQEQISTAQKELAQLLPWIDISQTEWSTLRIDRGEPLQSGLTRPNKAFLAEHGRILTGWPTKLALAPDFADRVLNAIESCGVRPNASDALPDLPKPVFSQPAWEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL76</accession>
    <name>A0A481QL76_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">rpsC</name>
        <name evidence="12" type="ORF">C3B55_00283</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00283"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_B">
        <property type="entry name" value="Ribosomal_S3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005704">
        <property type="entry name" value="Ribosomal_S3_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_S3_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01009">
        <property type="entry name" value="rpsC_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="11">
        <location>
            <begin position="39"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="237" mass="26453" checksum="CFCFD38BC7D53672" modified="2019-06-05" version="1">MGQKVHPIGIRLGIVKEHTSVWYADGKTYAGYLFADLKVRAYLQDKLKSASVSRIDIHRPAQTARITIHTARPGILIGKKGEDVEKLRNDLIKQMGVPVNINIEEIRKPELDGMLVAQSVAQQLERRVMFRRAMKRSVQNAMRIGAKGIKIQVSGRLGGAEIARTEWYREGRVPLHTLRADIDYANYEAHTTYGVIGVKVWIFKGEVIGGRQEALKLQAPVPRKKLPSKGGAPNVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL82</accession>
    <name>A0A481QL82_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00201"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="45" mass="5040" checksum="F84A6F01B9FBEC29" modified="2019-06-05" version="1">MVTKDYTLLQLNSPSNLDTIYVSKRCAAEWISDILVMATEIACFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL85</accession>
    <name>A0A481QL85_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease R</fullName>
            <shortName evidence="6">RNase R</shortName>
            <ecNumber evidence="6">3.1.13.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">rnr</name>
        <name evidence="9" type="ORF">C3B55_00235</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.13.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RNR ribonuclease family. RNase R subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.1" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00235"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01895">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040476">
        <property type="entry name" value="CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013223">
        <property type="entry name" value="RNase_B_OB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004476">
        <property type="entry name" value="RNase_II/RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011805">
        <property type="entry name" value="RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17876">
        <property type="entry name" value="CSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08206">
        <property type="entry name" value="OB_RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00358">
        <property type="entry name" value="3_prime_RNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02063">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="667"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="764"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="771"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="804" mass="90776" checksum="729C7245EC4FF662" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL86</accession>
    <name>A0A481QL86_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">rpsK</name>
        <name evidence="9" type="ORF">C3B55_00301</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00301"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019981">
        <property type="entry name" value="Ribosomal_S11_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03632">
        <property type="entry name" value="uS11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="129" mass="13619" checksum="D29E17C188641011" modified="2019-06-05" version="1">MTKPAASPRKKVKKIVVDGIAHIHASFNNTIVTITDRQGNALSWATSGGSGFRGSRKSTPFAAQVAAERAGQAALEYGLKNLDVNVKGPGPGRESAVRALNGCSYKIASITDVTPIPHNGCRPPKKRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL95</accession>
    <name>A0A481QL95_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Shikimate kinase</fullName>
            <shortName evidence="10">SK</shortName>
            <ecNumber evidence="3 10">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">aroK</name>
        <name evidence="11" type="ORF">C3B55_00186</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the shikimate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="3 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00186"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="172" mass="19524" checksum="AFDF320DF1E6E779" modified="2019-06-05" version="1">MQNLIFVGPMGAGKSTIGRLLAKELRMPFKDSDKEIELRSGANVPWIFDKEGEIGFRNREQAMIAELCGCNGVVLATGGGAVIRDENRRTLYIGGRVVYLRASVEQQVGRTYRDYSRPLLHTIDPARTMRDLLVQRDPLYREIADLVVETDERPLRIVVCDILERLQQLPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLC2</accession>
    <name>A0A481QLC2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rhdA</name>
        <name evidence="3" type="ORF">C3B55_00222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00222"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="21"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="165"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="271" mass="29944" checksum="93B2206CCB83D7AB" modified="2019-06-05" version="1">MPDFSGLPLVIEPSDLFGQLNAEHLILVDLTRSTRYTAGHIPGARFVDPRRTQLGQPMAAGLLPHKASLETLFGELGHTQDATYVVYDDEGGGWAGRFIWILDVIGHQKYHYLDGGLLAWLEAKHPVSTKVPSRVRGPVSLTLHEGPTATREYLQSRLGATDLGIWDARDPLEYSGKIVLAAKAGHIPGAVNLEWTAGMDKLHSLRIRRNMPQILESLGLTSDKEIITHCQTHHRSGFTYLLAKAFGYPRVKGYAGSWSEWGNHPSTPIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLC5</accession>
    <name>A0A481QLC5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00343"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="29" mass="3298" checksum="68E9CC590D120A5F" modified="2019-06-05" version="1">MMFSKAEILGKSLQSRAAVRDFLDIINDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLD5</accession>
    <name>A0A481QLD5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.345</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pimB</name>
        <name evidence="3" type="ORF">C3B55_00207</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.345" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00207"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043750">
        <property type="term" value="F:phosphatidylinositol alpha-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="27"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="189"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="384" mass="43225" checksum="018F00B0889C01C5" modified="2019-06-05" version="1">MSRHFRVLQLQPDYNVKDYDCADLAEQIIKALPTDRYEVTATFLRGRPGPTEPVSRAAKSAYFELSNSALKGLRLRAMWQLYKFCRREKFDVVICNRFKPVNMMLQLNRWLKIPLCIGISHGFGEYDRLYRRKQVQCWLDEHWHFIGVSPAVTQYLLNLKCGFTYQNTHTIKNAIDIKQSVGLQHSRKRARELLGINPTVRLIGALGRLVPVKGHTYLVQAFAALKDQYPTAQLAIIGTGREKSHLITQIQDLGLAGRVQLLGFKENALQYVQAFNIWAIPSLSEGSCLALLEGMSGRLPVIASSVPAMLPLIQSAGGLAVNPANVSELTMALDAYLALSDEALARKGEQAYRCLQAQHDIMVFRKEYLELIDSKLAQACGKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLE2</accession>
    <name>A0A481QLE2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9 10">Translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">infB</name>
        <name evidence="13" type="ORF">C3B55_00374</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00374"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013575">
        <property type="entry name" value="IF2_assoc_dom_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08364">
        <property type="entry name" value="IF2_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="352"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="G-domain" evidence="9">
        <location>
            <begin position="355"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="361"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="407"/>
            <end position="411"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="461"/>
            <end position="464"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="856" mass="93105" checksum="9209F1473212FB2E" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLF6</accession>
    <name>A0A481QLF6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00506"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="1">
        <location>
            <begin position="90"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="387" mass="44101" checksum="2618B1B0EE083850" modified="2019-06-05" version="1">MGEFDTIRPYNDSEVPAVLARLFSDKAFLDILTHFRFPRFAGTLGWLLKSMIARKLRREFAGVTTVATLQDKVEYYVDHTIDRATDGVIYTGVEQLKFGTAYLFLANHRDIVMDPAFVNYGVYHLGLPTPRIAIGDNLLQKPFVSDLMRLNKSFIVHRSITGRKEKMAAYILLSTYINHSIRNDCQSIWIAQAEGRAKDGDDRTESAILKMFHVSRKDEPFAEVIQSLNLTPVSISYEYDPCDIAKARELYIRATTGTYRKAYGEDDVSIALGITGYKGRVHVNFAPSIIEKFEDTKLLAIEIDRRILGGYWLFPVHYLAYAQWRDADPQLQVPTAAEVFPADELAKAKAEWEFRLSECPNEHRPFLVAQYATPVKNQYRIKAGIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLG0</accession>
    <name>A0A481QLG0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">30S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">rpsR</name>
        <name evidence="8" type="ORF">C3B55_00238</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00238"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00270">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_S18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018275">
        <property type="entry name" value="Ribosomal_S18_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_S18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="PTHR13479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="SSF46911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00165">
        <property type="entry name" value="S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00057">
        <property type="entry name" value="RIBOSOMAL_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="76" mass="9002" checksum="8F017406C942ABCE" modified="2019-06-05" version="1">MARFFRRRKFCRFTAENVKEIDYKDLNTLRVYISETGKIVPSRITGTKARYQRQLATAIKRARFLALLSYTDSHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLG6</accession>
    <name>A0A481QLG6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phospholipid ABC transporter-binding protein MlaD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mlaD</name>
        <name evidence="2" type="ORF">C3B55_00518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00518"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030970">
        <property type="entry name" value="ABC_MlaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003399">
        <property type="entry name" value="Mce/MlaD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02470">
        <property type="entry name" value="MlaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04430">
        <property type="entry name" value="OM_asym_MlaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MlaD" evidence="1">
        <location>
            <begin position="38"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="155" mass="16648" checksum="621585E22E7B8B63" modified="2019-06-05" version="1">MKNCTVEISVGLFLLAGILSLLLLALRVSGPMTSHTADAYKLYVYFNNIAGLTVRAKVTMAGVTIGKVTAIDLDHDSFTGRVTMHIDNKVDNLPTDSTASILTAGLLGEKYIDISVGGEPSLLKDGSIIHNTQSALVLEDLISKFLFNTVHKDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLH4</accession>
    <name>A0A481QLH4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="8">3-dehydroquinase</shortName>
            <ecNumber evidence="6 8">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Type II DHQase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">aroQ</name>
        <name evidence="12" type="ORF">C3B55_00252</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Catalyzes a trans-dehydration via an enolate intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Homododecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the type-II 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="6 8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00252"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00466">
        <property type="entry name" value="DHQase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.9100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00169">
        <property type="entry name" value="AroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001874">
        <property type="entry name" value="DHquinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018509">
        <property type="entry name" value="DHquinase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036441">
        <property type="entry name" value="DHquinase_II_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272">
        <property type="entry name" value="PTHR21272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01220">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001399">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52304">
        <property type="entry name" value="SSF52304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01088">
        <property type="entry name" value="aroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01029">
        <property type="entry name" value="DEHYDROQUINASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 9">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8 11">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="155" mass="17245" checksum="849EE8F7946A3126" modified="2019-06-05" version="1">MATLLVLHGPNLNLLGTRESEIYGTVTLKQINFDLERQAHEAGHHLFCLQSNAEHELINCLHTAHSEGLDFILINPAAFTHTSIALRDALLAINIPFIEVHLSNMHNREAFRHYSYFSDLAVGVICGLGASGYRLALEAALEQIKQLAKRHLTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLJ6</accession>
    <name>A0A481QLJ6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome-binding factor A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">rbfA</name>
        <name evidence="3" type="ORF">C3B55_00375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer. Binds 30S ribosomal subunits, but not 50S ribosomal subunits or 70S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RbfA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00375"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00003">
        <property type="entry name" value="RbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000238">
        <property type="entry name" value="RbfA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023799">
        <property type="entry name" value="RbfA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020053">
        <property type="entry name" value="Ribosome-bd_factorA_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33515">
        <property type="entry name" value="PTHR33515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02033">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89919">
        <property type="entry name" value="SSF89919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00082">
        <property type="entry name" value="rbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01319">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="131" mass="14896" checksum="B73E0B5858BB1F46" modified="2019-06-05" version="1">MAKEYNRTQRIGYQIQCELAQLIYREVKDPRIGLVTITTVEVSRDVGYAKIFITVMGQNNNKEIAQNIKALNSAVGFLRMQLAREINLRSVPQLHFQYDESIARGARLSALIECAVAVDSQHPYIPKDTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLK5</accession>
    <name>A0A481QLK5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00330"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="88" mass="9877" checksum="8DD1F971293B8977" modified="2019-06-05" version="1">MINTAVEVKVMVSNIGFLNIDHSSKAMSNPNKDNKDKILSIVRKYVKINDNLADEHYTIVQLHIAATVQYQSYRLNSVPATNLACMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLK9</accession>
    <name>A0A481QLK9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chaperone SurA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase SurA</fullName>
            <shortName evidence="5">PPIase SurA</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Rotamase SurA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">surA</name>
        <name evidence="8" type="ORF">C3B55_00119</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Chaperone involved in the correct folding and assembly of outer membrane proteins. Recognizes specific patterns of aromatic residues and the orientation of their side chains, which are found more frequently in integral outer membrane proteins. May act in both early periplasmic and late outer membrane-associated steps of protein maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Periplasm</location>
        </subcellularLocation>
        <text evidence="5">Is capable of associating with the outer membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The PPIase activity resides only in the second parvulin domain. The N-terminal region and the C-terminal tail are necessary and sufficient for the chaperone activity of SurA. The PPIase activity is dispensable for SurA to function as a chaperone. The N-terminal region and the C-terminal tail are also required for porin recognition.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00119"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01183">
        <property type="entry name" value="Chaperone_SurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023034">
        <property type="entry name" value="PPIase_SurA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015391">
        <property type="entry name" value="SurA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09312">
        <property type="entry name" value="SurA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 5" id="KW-0574">Periplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5 6" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019874194" description="Chaperone SurA" evidence="5">
        <location>
            <begin position="29"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="7">
        <location>
            <begin position="179"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="7">
        <location>
            <begin position="289"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="441" mass="49946" checksum="A4DEFBCE24F72197" modified="2019-06-05" version="1" precursor="true">MNVKIKLSNCLQLLALGALLIETASTHATVRQLDKIVAIVDNDVIMQSQLDYRVKEVQHTIAKRSGNVLPTTVLIPQILEHLIIENLQLQIGERSGIHISNEELNQTLNTVAQHNNMSIDQFKATLTHDGLSYKNTLDKIKNEMIINRVRQYKVAKYIHVSEQEVQNFLTSDFVKMQLSEELHLANILIPITNSTNAEQFNAAVTKAQAVYAHLKAGADFAQTAVAVSNSDSALSGGDIGWRKAVQLPPPLDRKLSTMKVGEITQPARMPGGFIILKLLEKRNNEALLKNEVHVRHILIKPNNIRTEQHAKELATKIYNRIKNGEDFAFLAKTFSEDPSSSLDGGDLHWIDLKALVLEFQDVILKIPQGVLSKPFRTQYGWHIMEVLDHRTTDNTIKALEQQAMNILRNRKYDIELQNWLHQIRDESYVENKLLNNYQAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLL4</accession>
    <name>A0A481QLL4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome-binding ATPase YchF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">ychF</name>
        <name evidence="7" type="ORF">C3B55_00400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00400"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00092">
        <property type="entry name" value="TIGR00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="3"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="6">
        <location>
            <begin position="281"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="366" mass="40467" checksum="40F28C91DEF46325" modified="2019-06-05" version="1">MGFNCGIVGLPNAGKSTLFNALTKSGIAAENFPFCTIEPNIGIVPMPDLRLKALAAIVNPKRIITTSMEFVDIAGLVAGASKGAGLGNKFLAHIRETDAIAHVVRCFEDENVMHVSNSIDPKHDIEIINLELIFADLDSCKKQLHKVARNAKSGDKKIVIQKSLLEQLITHLAKNKPARSLIKNMDAYEKIIFKNFHLLTTKPVMYIANVTEDGFEKNPLLDIVKAISATEDVITIPVCNKIEAEIASFEEGEDKKIYLEALGLQEFGLNRLISAGYKLLNLQTYFTVGIKEVRAWTVKVGTTAPQAASVIHTDFEKGFIRAEVIAYNDFIQCNGETGAKEAGKWRLEGKEYIVRDGDIMRFRFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLM6</accession>
    <name>A0A481QLM6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Elongation factor 4</fullName>
            <shortName evidence="7">EF-4</shortName>
            <ecNumber evidence="7">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ribosomal back-translocase LepA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">lepA</name>
        <name evidence="9" type="ORF">C3B55_00559</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00559"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="PTHR43512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="5"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="599" mass="66486" checksum="EC8E32A85259D77E" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLQ1</accession>
    <name>A0A481QLQ1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipopolysaccharide export system protein LptC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lptC</name>
        <name evidence="7" type="ORF">C3B55_00522</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. Facilitates the transfer of LPS from the inner membrane to the periplasmic protein LptA. Could be a docking site for LptA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the lipopolysaccharide transport and assembly complex. Interacts with LptA and the LptBFG transporter complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the LptC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00522"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015221">
        <property type="term" value="F:lipopolysaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01915">
        <property type="entry name" value="LPS_assembly_LptC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010664">
        <property type="entry name" value="LipoPS_assembly_LptC-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026265">
        <property type="entry name" value="LptC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06835">
        <property type="entry name" value="LptC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04409">
        <property type="entry name" value="LptC_YrbK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="190" mass="21739" checksum="25D94853239C0A5F" modified="2019-06-05" version="1">MLSKKILNFLLPGISIALFVVVRYLNISPELFFDNHVAHIDENVIDYYVTHAYSMQFLPDGKVQYEMTSDKVEHLKDSKISLLTNPDMKLYRGTEFPWHVTSQHGEIKPDGTQVELIELVRVARTDQKNHNIIITSSSMTVFLQQQYAQTEQDVRIDFGGSVLIGKGMKAYLKNSSLDLLSNVQGKYEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLQ8</accession>
    <name>A0A481QLQ8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00600"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="69" mass="7832" checksum="17CE75BD83ABF491" modified="2019-06-05" version="1">MIVIYSKPDPLPLLNECGGLLANYSNSCLRNFNNSLLDASVMMWIKQSSYFLVETQLIGVVLKEFQFIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLR0</accession>
    <name>A0A481QLR0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Cell shape-determining protein MreC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 5">Cell shape protein MreC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mreC_2</name>
        <name evidence="6" type="ORF">C3B55_00532</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in formation and maintenance of cell shape.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MreC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00532"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042177">
        <property type="entry name" value="Cell/Rod_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042175">
        <property type="entry name" value="Cell/Rod_MreC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007221">
        <property type="entry name" value="MreC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34138">
        <property type="entry name" value="PTHR34138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04085">
        <property type="entry name" value="MreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038471">
        <property type="entry name" value="MreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00219">
        <property type="entry name" value="mreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="311" mass="34314" checksum="2ACBB435B5A22F35" modified="2019-06-05" version="1">MLLITLMFIDARFTLLKPVRNQMALVLMQTYWITDLPQRLYHSVANQFGSRTELVAENAKLKTENLLLQGRIQKLAAITEQNVQLRELLKSSALVNEKVEVAELIGIDPNPFTHRIILNKGERDGVVLGQPVLDARGLIGQVVELMPYTSRVLLLTDTTHSIPVQVNRNGLRAIASGTGNPERLELRHVADTADIQEGDLLVSSGLGQRFPAGYPVATVNEVIHYSGQPFAIIRAIPTAAINSSRYLLLVYSDNCATEERINYASQAKGIEDQPNNTCAKHSDHSTATDIGWPTCIYYHTDRSASCGSPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLS5</accession>
    <name>A0A481QLS5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">3-deoxy-D-manno-octulosonic acid transferase</fullName>
            <shortName evidence="9">Kdo transferase</shortName>
            <ecNumber evidence="3 9">2.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 9">Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">waaA</name>
        <name evidence="11" type="ORF">C3B55_00216</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E. coli) = alpha-Kdo-(2-&gt;6)-lipid IVA + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28066"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58603"/>
            <dbReference type="ChEBI" id="CHEBI:60364"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:85987"/>
            <dbReference type="EC" id="2.4.99.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Bacterial outer membrane biogenesis; LPS core biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.12" evidence="3 9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00216"/>
    <dbReference type="UniPathway" id="UPA00958"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043842">
        <property type="term" value="F:Kdo transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009244">
        <property type="term" value="P:lipopolysaccharide core region biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007507">
        <property type="entry name" value="Glycos_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038107">
        <property type="entry name" value="Glycos_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039901">
        <property type="entry name" value="Kdotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42755">
        <property type="entry name" value="PTHR42755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04413">
        <property type="entry name" value="Glycos_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_N" evidence="10">
        <location>
            <begin position="60"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR639901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR639901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="453" mass="50714" checksum="9E097C3272C725D6" modified="2019-06-05" version="1">MLYASRINDLSSSCRLFTEKPIRPTAMNRTLYSYLFYLALPLVLLRLWLRARKVPAYTKRVRERFAYSLPVMNHGGIWVHAVSVGEIIAAAPMIGGLIKRYPTLPITVTCMTLTGSEHIQILFAHEPRIQHCYLPYDLPCAAKRFLDHVQPELAVIMETELWPNHIHACAQRGIPVALANARMSARSVKCYARFAKLTSSMLAKINLFAVQTRTEAQYFLSLGARQETIDVTGSIKFNLTIDPQLPMRAAALREQWGASERKIWIAASTHEGEDKVILAAYRQLLENYPDALLILVPRHQERFSQILNLCRQQGFATVCRSSGEPVKARTSVLLGDTMGELLFLYALADIAFVGGSLVPAGGHNPLEPVALAIPVIMGIYSFNFFEISAMMRNAGALLEVHDAQELAEAVRQLFALPQDAHKMAEAGLKVMQANQGALKRLLDDLDELFDRYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLV5</accession>
    <name>A0A481QLV5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-deoxy-manno-octulosonate cytidylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">CMP-2-keto-3-deoxyoctulosonic acid synthase</fullName>
            <shortName evidence="2">CKS</shortName>
            <shortName evidence="2">CMP-KDO synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">kdsB</name>
        <name evidence="3" type="ORF">C3B55_00467</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3-deoxy-alpha-D-manno-oct-2-ulosonate + CTP = CMP-3-deoxy-beta-D-manno-octulosonate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23448"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:85986"/>
            <dbReference type="ChEBI" id="CHEBI:85987"/>
            <dbReference type="EC" id="2.7.7.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KdsB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.38" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00467"/>
    <dbReference type="UniPathway" id="UPA00358">
        <property type="reaction ID" value="UER00476"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008690">
        <property type="term" value="F:3-deoxy-manno-octulosonate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033468">
        <property type="term" value="P:CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02517">
        <property type="entry name" value="CMP-KDO-Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00057">
        <property type="entry name" value="KdsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003329">
        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004528">
        <property type="entry name" value="KdsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00466">
        <property type="entry name" value="kdsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="256" mass="28626" checksum="3D087807AC639A42" modified="2019-06-05" version="1">MTTAFTVIIPSRYASTRLPGKPLRFIGNKPMIQLVWEQTCKSGAERILVATDDQRIIEVCKGFGAEAVLTLEDHNSGTDRLAEVAVQLGLAADAIVVNVQVDEPLIPPCLIDQVAANLEAYGEARIATLAEPIKDIDTLFSPNVVKVVSDTNGLALTFSRSILPWSRDCFIKNLDVLPKGVLYRRHIGIYAYRAGFLYDFINWSPCWLEKTESLEQLRALWYGVRIHVDDALETLPAGVDTKEDLERIRCLLWQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLW2</accession>
    <name>A0A481QLW2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00677</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00677"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="64" mass="7798" checksum="C887FE738CFD313C" modified="2019-06-05" version="1">MNINNIKLRNQRHEVKKLFLLNFIYVVQVNLGLWDLLSKITINVDNICIKYFSIKVYRRIQYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLX4</accession>
    <name>A0A481QLX4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADH-quinone oxidoreductase subunit A</fullName>
            <ecNumber evidence="7">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">NADH dehydrogenase I subunit A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">NDH-1 subunit A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">NUO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ndhC</name>
        <name evidence="7" type="synonym">nuoA</name>
        <name evidence="9" type="ORF">C3B55_00447</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cell membrane</location>
            <topology evidence="7 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the complex I subunit 3 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00447"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01394">
        <property type="entry name" value="NDH1_NuoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023043">
        <property type="entry name" value="NAD(P)H_OxRDtase_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000440">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038430">
        <property type="entry name" value="NDAH_ubi_oxred_su3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058">
        <property type="entry name" value="PTHR11058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00507">
        <property type="entry name" value="Oxidored_q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7 8" id="KW-0874">Quinone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="129" mass="14365" checksum="2C2B1B0942E1607D" modified="2019-06-05" version="1">MNEATELIAHDWGFAIFILSAISFCILIISISSLLGSKAFGRSKNEPFESGMLPTGSARLRLSAKFYLVAMLFVIFDIETIFLLIWSVSVRESGWTGFVEALVFIAILLAGLIYLIRVGALDWTTKVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLY9</accession>
    <name>A0A481QLY9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arabinose 5-phosphate isomerase</fullName>
            <shortName evidence="2">API</shortName>
            <ecNumber evidence="2">5.3.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">kdsD</name>
        <name evidence="8" type="ORF">C3B55_00521</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-arabinose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23104"/>
            <dbReference type="ChEBI" id="CHEBI:57693"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.3.1.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SIS family. GutQ/KpsF subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00521"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019146">
        <property type="term" value="F:arabinose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05014">
        <property type="entry name" value="SIS_Kpsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004800">
        <property type="entry name" value="KdsD/KpsF-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035474">
        <property type="entry name" value="SIS_Kpsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004692">
        <property type="entry name" value="KdsD_KpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00393">
        <property type="entry name" value="kpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="2 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SIS" evidence="7">
        <location>
            <begin position="36"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="205"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="272"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR004692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004692-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR004692-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="324" mass="34548" checksum="EB4DE85B599694F0" modified="2019-06-05" version="1">MSQSSNLIRSAQRTIRLELKAVEGLLVHIDADFVRACEMILASKGRVVVVGMGKSSHVGNKIAATLASTGTMAFFVHPAEASHGDMGMIAKDDIILAISNSGMTSEIVTLLPLIKRLGIKLISLSGNQESMLTKAAEVNLNVRVAYEACPLNLAPTSSTTTALVMGDALAVALLEARGFTAQDFAFLHPGGTLGRSLLLKVENVMHSGNELPHVRLGTLLRDALIEMTCKSLGMTVIIDANGRLAGVFTDGDLRRSLDRHIDIHTAIVDAVMTPHGKTAHAEMLVTEALKIMEEYKISALVVVNSDNCPIGVLNMHDLLRAGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM09</accession>
    <name>A0A481QM09_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydro-3-deoxyphosphooctonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.55</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-deoxy-D-manno-octulosonic acid 8-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">KDO-8-phosphate synthase</fullName>
            <shortName evidence="6">KDO 8-P synthase</shortName>
            <shortName evidence="6">KDOPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyoctonate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">kdsA</name>
        <name evidence="8" type="ORF">C3B55_00753</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>D-arabinose 5-phosphate + H2O + phosphoenolpyruvate = 3-deoxy-alpha-D-manno-2-octulosonate-8-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14053"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57693"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:85985"/>
            <dbReference type="EC" id="2.5.1.55"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 6">Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the KdsA family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.55" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00753"/>
    <dbReference type="UniPathway" id="UPA00030"/>
    <dbReference type="UniPathway" id="UPA00357">
        <property type="reaction ID" value="UER00474"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008676">
        <property type="term" value="F:3-deoxy-8-phosphooctulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019294">
        <property type="term" value="P:keto-3-deoxy-D-manno-octulosonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00056">
        <property type="entry name" value="KDO8P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006269">
        <property type="entry name" value="KDO8P_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21057">
        <property type="entry name" value="PTHR21057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01362">
        <property type="entry name" value="KDO8P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP_synth_1" evidence="7">
        <location>
            <begin position="10"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="281" mass="31007" checksum="913E0D236AB69512" modified="2019-06-05" version="1">MVQKIIRVGDIEIANDKSIVLFGGINVLESGDMAMHVCEEYIKVTQKLNIPYVFKASFDKANRSSITSYRGPGLEEGMRIFQNIKQAFGVPIITDVHEPWQAAVVAEMCDIIQLPAFLSRQTDLVVAMAKTGAVINIKKAQFLAPHEIKHILNKFVEAGNDQLIFCERGSSFGYNNLVVDMLSFGIMKQFQYPVFFDVTHALQMPGSRINSAGGRRAQVLDLAKAGISQSLAGLFLEAHPDPDNAKCDGPCALRLDNLEPFLAQLKHLDALIKSFPAIETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM12</accession>
    <name>A0A481QM12_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00741"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="75" mass="8423" checksum="117E7A35A9425DDB" modified="2019-06-05" version="1">MPSSHKIIKINLYFLSTFIYFATIMAQIKIVRDYTSFKVPLQSFSKLVHSNPIAAHIAALIVKLIISSKMITGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM15</accession>
    <name>A0A481QM15_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00552"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="70" mass="7614" checksum="F4CDCA05B435C6B6" modified="2019-06-05" version="1">MAISAKSLYTISSLVILESIRIFLASYASIVVHGNIAQYEIFYTISGVVVYSVSFPVSLNKRASIAILLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM26</accession>
    <name>A0A481QM26_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00773</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00773"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="77" mass="8777" checksum="92A9632EA222FD30" modified="2019-06-05" version="1">MQCLAKQYYSVIKCDQFTPPLIQGLYLDVLVAIVIFDSARVNFVVCYPVHDMSCYALDSFILAICILPYQDANQYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM32</accession>
    <name>A0A481QM32_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatC</name>
        <name evidence="2" type="ORF">C3B55_00534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00534"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="PTHR15004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="95" mass="10501" checksum="487831DA903F544C" modified="2019-06-05" version="1">MTLDHSDVERIAHLASLGLNYTYLSQTTAALNSILRLVNQIQDIDTGSIEPLAHPLESRQRLCVDVVTEQNNRASYQAIAPAVEKGLYLVPKVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM33</accession>
    <name>A0A481QM33_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 9">Dihydroorotase</fullName>
            <shortName evidence="8">DHOase</shortName>
            <ecNumber evidence="4 8 9">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pyrC_2</name>
        <name evidence="8" type="synonym">pyrC</name>
        <name evidence="11" type="ORF">C3B55_00784</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 9">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8 9">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="4 8 9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00784"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01294">
        <property type="entry name" value="DHOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00219">
        <property type="entry name" value="PyrC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004721">
        <property type="entry name" value="DHOdimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137">
        <property type="entry name" value="PTHR43137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001237">
        <property type="entry name" value="DHOdimr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00856">
        <property type="entry name" value="pyrC_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="10">
        <location>
            <begin position="12"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="16"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="8">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="8">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="348" mass="39046" checksum="A823EF4B798D9FEC" modified="2019-06-05" version="1">MSNYITMLRPDDWHIHLRDGAALPQTVADVARTFSRAIIMPNIVPPVCNTIEAKAYRQRILAARPPGNYFEPLMVIYLTDHTQPKEISRAKATSFIHAAKLYPVGTTTNSDSGVTSLDKILLAIEAMAKVGMPLLIHGEVTRCDVDVFDREKIFIDEHMCRIVELFPTLKVVLEHITTTDAVQFVNEASANVSATITAHHLLYNRNHMLANRIRPHFYCLPILKRNTHQIALLDAATSGNPKFFLGTDSAPHARHVKEAMYGCAGCYTAHTAIALYAEAFEQRNALDKLESFASFNGPRFYGLPVNTDKITLVRENWTAPASLPFGEQTIIPLRANEILRWRLLEESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM43</accession>
    <name>A0A481QM43_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TIGR02099 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">C3B55_00529</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00529"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032712">
        <property type="entry name" value="AsmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011836">
        <property type="entry name" value="CHP02099"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025263">
        <property type="entry name" value="DUF3971"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38690">
        <property type="entry name" value="PTHR38690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13502">
        <property type="entry name" value="AsmA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13116">
        <property type="entry name" value="DUF3971"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02099">
        <property type="entry name" value="TIGR02099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1215"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3971" evidence="2">
        <location>
            <begin position="517"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="AsmA_2" evidence="3">
        <location>
            <begin position="1016"/>
            <end position="1260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="1267" mass="140510" checksum="89ECFCA2323056DF" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM58</accession>
    <name>A0A481QM58_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00549"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="66" mass="7649" checksum="75A16CC4D3D84E32" modified="2019-06-05" version="1">MSQSHVLYSCDNLCIMWYAVLTFHNLVIQKPINYLMFTSNYNFGKTLPCILMIMLSTNEAIISIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM66</accession>
    <name>A0A481QM66_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">UDP-N-acetylmuramate--L-alanine ligase</fullName>
            <ecNumber evidence="4 11">6.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">UDP-N-acetylmuramoyl-L-alanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 16" type="primary">murC</name>
        <name evidence="16" type="ORF">C3B55_00575</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine</text>
            <dbReference type="Rhea" id="RHEA:23372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.8" evidence="4 11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00575"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008763">
        <property type="term" value="F:UDP-N-acetylmuramate-L-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00046">
        <property type="entry name" value="MurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005758">
        <property type="entry name" value="UDP-N-AcMur_Ala_ligase_MurC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01082">
        <property type="entry name" value="murC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 11" id="KW-0133">Cell shape</keyword>
    <keyword evidence="10 11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase" evidence="13">
        <location>
            <begin position="18"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="15">
        <location>
            <begin position="121"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="14">
        <location>
            <begin position="321"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="123"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX81908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="478" mass="52040" checksum="F5E4D9747C8C0FFD" modified="2019-06-05" version="1">MIKSQTVVPQPEMRRIRRIHFVGIGGASMCGIAEVLLSLGYQVSGSDLKKSSVTDRLKTFNAKIFIGHRAENVAEADVLVVSSAVTTSNPEVVTALERRIPVVPRAEMLAELMRYRHGIAVAGTHGKTTTTSLIASVFAADGLDPTFVIGGCLNAVGTNAQLGTSRYLIAEADESDTSFLHLQPLVAVVTNIDLDHMRTYDGDCNKLKKAFIEFIHNLPFYGLAVVCLDDPIACEILPQIKRPTMTYGFSEVADVRAINVCQKGMQTFFTVLRQDRQPLNVSVNMPGNHNVLNSLATICIASDEGVSDAAIIKGLSCFSGVGRRFQIYGQLSIEGGNVMLVDDYGHHPTEVAAVMKAIRSGWSGRRLVMVYQPHRYSRTCDLYDDFVNVLADANVLLLMEVYSAGEEPIPGANSLRMCNSIRQCSQLDPIYIERGVDLAPIVKRLLRVGDILLCQGAGDIGSLAPKLLASPLFRIAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM67</accession>
    <name>A0A481QM67_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Lipopolysaccharide export system permease protein LptG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">lptG</name>
        <name evidence="10" type="ORF">C3B55_00560</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex LptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the lipopolysaccharide transport and assembly complex. The LptBFG transporter is composed of two ATP-binding proteins (LptB) and two transmembrane proteins (LptF and LptG).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LptF/LptG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00560"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030923">
        <property type="entry name" value="LptG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005495">
        <property type="entry name" value="LptG/LptF_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33529">
        <property type="entry name" value="PTHR33529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03739">
        <property type="entry name" value="LptF_LptG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04408">
        <property type="entry name" value="LptG_lptG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="360" mass="39972" checksum="9B510105A43D706F" modified="2019-06-05" version="1">MTKLDSYIGGSVLIYIMAVLGIILGLASLFAFIDEISNVTDTYTVIDVLSYVALTAPRRLYDLMPMAALIGCLISLGGLDSNSELIVMRTAGVSICRIVWSVMQPILLMMVFSVLIGEYVTPLTEAKAQANRTLARDSGDAQSAKHGLWHRHGNEFIHINAVQPGNLLVGVTRYTFDKERHMLASSFSKCAQYCDERRWELYEVTTTYFRNVGQGTKAYTEVINIPSEQWNIALQPELLNTVLMIPESLSISGLWGYIHYLKGQGLNNGRYWLAFWVKLSQPLVTATLVFMAISFVFGPLRAVTIGQRVFTGVLVGFTFRIAQDLLGQSSLVFGFSPLLTVLVPTIMCTLVGFWLLSRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM90</accession>
    <name>A0A481QM90_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00598"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="39" mass="4590" checksum="603A4A3E7819E154" modified="2019-06-05" version="1">MDRLYLEYHYATLWYAYLKSLYIFIAVGHVPAIIGQALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM95</accession>
    <name>A0A481QM95_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00652</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00652"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="31" mass="3553" checksum="215F3829AE9465D0" modified="2019-06-05" version="1">MDYFNPLVVTIVIVIYNFDTLDILDSHIGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMA2</accession>
    <name>A0A481QMA2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Antibiotic efflux pump outer membrane protein ArpC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">arpC</name>
        <name evidence="3" type="ORF">C3B55_00736</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00736"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="473" mass="52920" checksum="D8B000BC079CBAF9" modified="2019-06-05" version="1">MNRFLFFLAVILFLFSGCSLTPDYQRPKMPVATQYPHGQAYSLGQAAADQGWRHFFRDPSLQQLIQTALANNRDLHVASLNIDAYAAQYQIKRANLFPTFLTTSAGSHQRVPDKLLQTSKTGIIHQYSTNFSIDSYELDLLNRVHSLSEEALQKYLATEEARRSIHISLVASVANAYLTWQTGKELLKLTIDTLSTLEQGYKLTLRSNEVGMASALDVTRAHTFVENTRIQLAHYTRQVAQDENSLTMLLSTELPASIASKPLLTDLLSEVPAGLPSDLLQRRPDIMQAEYNLKAANANIGTARAAFFPSVRLTTSAGIASPSLSSLFKGNLGTWSFIPQVYIPIFSAGSLRASLDYSKIQKEINVANYEKAIQTGFQEVSNGLAARNTYKQQLDAQYSLVAANKDYYRLAKRRYHAGIDRKMTFLDAKRQLFNAQQSLITARLEQLKCEVNLYKALGGGWNEQTTKNELLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMA9</accession>
    <name>A0A481QMA9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome maturation factor RimP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">rimP</name>
        <name evidence="6" type="ORF">C3B55_00372</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for maturation of 30S ribosomal subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RimP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00372"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01734">
        <property type="entry name" value="YlxS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01077">
        <property type="entry name" value="RimP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003728">
        <property type="entry name" value="Ribosome_maturation_RimP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028998">
        <property type="entry name" value="RimP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036847">
        <property type="entry name" value="RimP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028989">
        <property type="entry name" value="RimP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035956">
        <property type="entry name" value="RimP_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33867">
        <property type="entry name" value="PTHR33867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02576">
        <property type="entry name" value="DUF150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17384">
        <property type="entry name" value="DUF150_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74942">
        <property type="entry name" value="SSF74942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75420">
        <property type="entry name" value="SSF75420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="DUF150" evidence="4">
        <location>
            <begin position="11"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="DUF150_C" evidence="5">
        <location>
            <begin position="86"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="152" mass="17304" checksum="AB2D935B8590AC0D" modified="2019-06-05" version="1">MSSKLKQLQALLAPVIVALGYEYWGIEFSAQRRHVILRIYIDKEGGVLVDNCVTVSRHISGVMDVEDLISVKYTLEVSSPGLERPLFTIEQFIKFIGKQVKIRLRSKFDGRRNFQGLLCGVEEQDIVVQVYDHELLLPIDIIDKANIVPSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMB5</accession>
    <name>A0A481QMB5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fe(3+) dicitrate transport system permease protein FecD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fecD</name>
        <name evidence="8" type="ORF">C3B55_00614</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00614"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="322" mass="34094" checksum="BBD2B3A51EA582B3" modified="2019-06-05" version="1">MTKPRWCLGLLLSLLLLALFVSLSAGTVWLKSATVLDRLLAHDALDFEVWNHRLPRSLIAILAGYAFGMAGAIVQGVIRNPLASPEILGVTQGASLALTIAIITWPQMPIVWLPLVACLGGISVALLLALYNIGVSFSGIRLALSGVAIAVILSSVTEFLILSHPLDINVALLALTGSLWSHNWHHVALVLPFLLLIPLGLCLAKPLNLIALSDEAAHSLGTTLSWTRSWAMVCAVVLTSVGVGVIGPIGFISLVAPHIARQLVGGHHQYLMPAAMLIGALLLVLADTLGRTLIAPSEIPAGIFTVVIGAPYFLWLLARFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QME9</accession>
    <name>A0A481QME9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="13">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="13">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">pheS</name>
        <name evidence="15" type="ORF">C3B55_00788</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="13"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00788"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00281">
        <property type="entry name" value="Phe_tRNA_synth_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004188">
        <property type="entry name" value="Phe-tRNA_ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022911">
        <property type="entry name" value="Phe_tRNA_ligase_alpha1_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PTHR11538:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02912">
        <property type="entry name" value="Phe_tRNA-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="14">
        <location>
            <begin position="116"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX82106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="338" mass="37889" checksum="0693B21365CE92C1" modified="2019-06-05" version="1">MKNLEAIVAQALEAAQGAVDINVLEQIRVHYLGKNGELTKVVKTLGGLPVKERPQVGALINLAKERVIEVFNVRKALIKEANLAAKLAAESIDVTLPGRGQVSGGLHPITRTLERIEQFFTCIGYGIAEGPEVEDDYHNFEALNIPSHHPARSMHDTFYFNASMLLRTHTSPVQVRTMQANKPPIRIVCPGRVYRNDSDVTHSPMFHQIEGLLIDYDINFADLKGTIEEFLRVFFEKKLAVRFRPSFFPFTEPSAEVDIECVMCGSKGCRICNQTGWLEVMGCGMVHPNVLGISKIDSEEFSGFAFGMGVERLAMLRYGVNDLRLFFDNDLRFLAQFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMG5</accession>
    <name>A0A481QMG5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Octopine transport system permease protein OccM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">occM</name>
        <name evidence="7" type="ORF">C3B55_00897</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the binding-protein-dependent transport system permease family. HisMQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00897"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071705">
        <property type="term" value="P:nitrogen compound transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="19"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="232" mass="26305" checksum="9F5EFA9610EC1B25" modified="2019-06-05" version="1">MIFEYNVIWEAMPLYLGGLLITLKLFVISLFFGLLLALLLGLMRVSKQPIVNGAAWLYTYVIRGTPMLVQLFLIYYGLAQFAVIRQSFLWPFLSNATFCVCLAFAINTSAYTAEIIAGGLRTTPNGEIEAAKAIGMSRYKLYYRILLPSALRRVLPQYSNEVIMILQTTSLSSIVTLVDITGAARTVNAQYYLPFEAYITAGVFYLCLTFILVRLFKLAERRWLSYLAPRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMG7</accession>
    <name>A0A481QMG7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00545"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="112" mass="12860" checksum="82ACE71DE2CDAFB4" modified="2019-06-05" version="1">MSYEIQVEGETSPKLLLVNDSATFTYVIASTISRRSFLIYAVGSTEENFIFARTDRPGYIVLNTLVLTNAMSVRHLWWEHIQRALTKNRIISLPSLLLMFWECASIFCSTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMI9</accession>
    <name>A0A481QMI9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DnaA regulatory inactivator Hda</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hda</name>
        <name evidence="2" type="ORF">C3B55_00835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00835"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032297">
        <property type="term" value="P:negative regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017788">
        <property type="entry name" value="Hda"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03420">
        <property type="entry name" value="DnaA_homol_Hda"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_DnaA" evidence="1">
        <location>
            <begin position="14"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="235" mass="26659" checksum="B2B750EF8CF7D68C" modified="2019-06-05" version="1">MKPIQLPLGVRLSDDATFINYYPGANAAALGYVKRLCEADAGWTESLIYLWGKHNVGRTHLLQAACLRFEQIDEPAIYLPLAELIDQGIGIFDHLEQYELVCLDDLQAIAGRTDWEEALFHLFNRLRDSGRRLLITASTSPRELPVKLADLKSRLTLALIFQMHPLSDDVDKVRALQLCALRRGLHLTDEVGYFILTRGTRSMSALFDLLEQLDQASLQAQRKLTIPFLREILGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QML3</accession>
    <name>A0A481QML3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 9">GTPase Der</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GTP-binding protein EngA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">der</name>
        <name evidence="11" type="ORF">C3B55_00951</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">GTPase that plays an essential role in the late steps of ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00951"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00195">
        <property type="entry name" value="GTPase_Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031166">
        <property type="entry name" value="G_ENGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016484">
        <property type="entry name" value="GTP-bd_EngA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032859">
        <property type="entry name" value="KH_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14714">
        <property type="entry name" value="KH_dom-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006485">
        <property type="entry name" value="GTP-binding_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03594">
        <property type="entry name" value="GTPase_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51712">
        <property type="entry name" value="G_ENGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="EngA-type G" evidence="10">
        <location>
            <begin position="3"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G" evidence="10">
        <location>
            <begin position="191"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="56"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="118"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="197"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="244"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="309"/>
            <end position="312"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="472" mass="53587" checksum="77C2A455A0979295" modified="2019-06-05" version="1">MVPVIAIVGRPNVGKSTLFNRLTRTRDAIVGNLSGLTRDRHYGSAKWQGRFYIIIDTGGIFGNEHGMDKRMATQSLLAIEEANVVLLLVDAHVGYTVVDQMIGEHLRKHNKCFYLVANKTDNINPEQVCTEFSHMGMGDAIPIAATQGYGITKMLEIALRDFFKSDVEEKKIVVESKEVGCIYNQSKTDLIKIAIIGRPNVGKSTLVNCMLGEDRVIVYEQPGTTRDSIYIQFDRQGERYTLIDTAGVRKRGKIHDEVEKFSVIKTLQAIKDSNVVIFVIDAGEGAVDQDLHLLDFAIEAGRALVIAINKWDSMTLSRRDFVKIELQRRLFFVEFADIHFISALYGAGVSNLYPSIQNSFRSAVTRWPTNYLTRILEDAVDWHMPPTVNKRRIKLRYAHLGGVNPPIIVIHGNQLEKVPKSYMRYLENTYRRVLNLVGTPIRIWFKNSENPYESQYNALADRQVNKKRQGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMM2</accession>
    <name>A0A481QMM2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="9">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="9">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="9">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">folD</name>
        <name evidence="12" type="ORF">C3B55_00610</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5,10-methenyltetrahydrofolate + H2O = (6S)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="9"/>
    <dbReference type="EC" id="3.5.4.9" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00610"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="1 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THF_DHG_CYH" evidence="10">
        <location>
            <begin position="6"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="THF_DHG_CYH_C" evidence="11">
        <location>
            <begin position="124"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="285" mass="31220" checksum="81DD2A708CAE3563" modified="2019-06-05" version="1">MTAQLIDGKSIAASLRQQITKRVTERRLQYLRTPGLAMILIGSDPASQVYVSHKRKDCKEVGFISKAYDLPSETTQQTLTNLIDSLNDDPSIDGILLQLPLPEYLDASKLLEHIRPDKDVDGFHPYNVGRLAQRIPLLRPCTPKGIMTLLKSTSVNLYGLNAVVIGASNIVGRPMAMELLLAGCTVTITHRFTRDLVNHVRRADLVIIATGKPGLVKGEWIKEGAIVIDVGINRLEGGRLVGDVVYETALPRASWITPVPGGVGPMTRVCLLENTLYAAEKLHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMM3</accession>
    <name>A0A481QMM3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">50S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rplT</name>
        <name evidence="7" type="ORF">C3B55_00789</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the bacterial ribosomal protein bL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00789"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07026">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00382">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005813">
        <property type="entry name" value="Ribosomal_L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035566">
        <property type="entry name" value="Ribosomal_protein_L20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10986">
        <property type="entry name" value="PTHR10986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00453">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00062">
        <property type="entry name" value="RIBOSOMALL20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74731">
        <property type="entry name" value="SSF74731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01032">
        <property type="entry name" value="rplT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00937">
        <property type="entry name" value="RIBOSOMAL_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="116" mass="13208" checksum="E9B5E3D853FBF5DF" modified="2019-06-05" version="1">MARIKRGVISRKRHKKILKLAKGYYGARSRVFRAAKQAVIKAGQYAYRDRRQKKRQFRSLWIARINAGARINGLSYSSFIAGLKKASIEIDRKVLAELAVNQKVVFAAIVEKAKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMP6</accession>
    <name>A0A481QMP6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rpmI</name>
        <name evidence="6" type="ORF">C3B55_00790</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bL35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00790"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00514">
        <property type="entry name" value="Ribosomal_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018265">
        <property type="entry name" value="Ribosomal_L35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001706">
        <property type="entry name" value="Ribosomal_L35_non-mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_L35_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33343">
        <property type="entry name" value="PTHR33343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00064">
        <property type="entry name" value="RIBOSOMALL35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="SSF143034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00001">
        <property type="entry name" value="rpmI_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00936">
        <property type="entry name" value="RIBOSOMAL_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="64" mass="7317" checksum="1398551D7AFBD920" modified="2019-06-05" version="1">MPKMKTKSSASKRFLKTANGIKHKHACKNHILTKMSTKRKRQLRGSSLLNPSEVAKVERILRLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMP7</accession>
    <name>A0A481QMP7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="11">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 15" type="primary">metG</name>
        <name evidence="15" type="ORF">C3B55_00782</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00782"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="PTHR45765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="SSF57770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-synt_1g" evidence="13">
        <location>
            <begin position="7"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_3" evidence="14">
        <location>
            <begin position="419"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="331"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX82100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="565" mass="63780" checksum="0901FA63F7496BE9" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMP9</accession>
    <name>A0A481QMP9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">engB</name>
        <name evidence="10" type="ORF">C3B55_00993</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00993"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03598">
        <property type="entry name" value="GTPase_YsxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0717">Septation</keyword>
    <feature type="domain" description="EngB-type G" evidence="9">
        <location>
            <begin position="30"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="213" mass="24153" checksum="6654359D5BC55637" modified="2019-06-05" version="1">MQFKNSILRLCQQSKFMLSAAKVNQYPDDQGFEIAFAGRSNAGKSSALNTLTSINLAHTSKTPGRTQLLNFFKLDDNRRLVDLPGYGYAKVPIQVKLHWQHRLEGYLNGRRSLKGLIITIDIRNLMNDFDLLMLNWAIARNMPIHILLTKADKLSYSAAKDTFLKVQSEIHQSLSDVITIQLFSAPKRIGLKDAYTILSSWMQLADKSTHITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMQ2</accession>
    <name>A0A481QMQ2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiol:disulfide interchange protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dsbA</name>
        <name evidence="6" type="ORF">C3B55_00992</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00992"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03019">
        <property type="entry name" value="DsbA_DsbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023205">
        <property type="entry name" value="DsbA/DsbL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001488">
        <property type="entry name" value="Tdi_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0574">Periplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019827200" description="Thiol:disulfide interchange protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="DSBA" evidence="5">
        <location>
            <begin position="98"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="3">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR001488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001488-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="226" mass="25083" checksum="539CC20489117524" modified="2019-06-05" version="1">MRTLILSAVLITASLCSMTAQAANNMLIEAGKTYVELAHPIPVLELDKIEVVELFWYGCSHCYALEPTINLWVRRLPSDVNFKRIPAMFGGLWDVHGQLFLTLEAMSAAHKVHDAVFSAIQKEGNSLTKLEDMVNFVITQGIDKDKFLAIFNSFAIHNQITQVKKLVQNCGVQGVPTIIINGKYRFDLGTTGSPEQTLNIADQLIIKERAAKSEMARLTGDAQRAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMQ5</accession>
    <name>A0A481QMQ5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="12">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 15" type="primary">thrS</name>
        <name evidence="15" type="ORF">C3B55_00792</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00792"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="PTHR11451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TGS" evidence="14">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="13">
        <location>
            <begin position="242"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Catalytic" evidence="12">
        <location>
            <begin position="242"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX82110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="640" mass="72839" checksum="53E085ED6EE3B046" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMR2</accession>
    <name>A0A481QMR2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease T</fullName>
            <ecNumber evidence="5">3.1.13.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Exoribonuclease T</fullName>
            <shortName evidence="5">RNase T</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rnt</name>
        <name evidence="7" type="ORF">C3B55_00785</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds two Mg(2+) per subunit. The active form of the enzyme binds two Mg(2+) ions in its active site. The first Mg(2+) forms only one salt bridge with the protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNase T family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00785"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016896">
        <property type="term" value="F:exoribonuclease activity, producing 5'-phosphomonoesters"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06134">
        <property type="entry name" value="RNaseT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00157">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005987">
        <property type="entry name" value="RNase_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30231:SF2">
        <property type="entry name" value="PTHR30231:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01298">
        <property type="entry name" value="RNaseT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="6">
        <location>
            <begin position="29"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate binding and specificity" evidence="5">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate binding and specificity" evidence="5">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate binding and specificity" evidence="5">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate binding and specificity" evidence="5">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="217" mass="23982" checksum="D8C4A27C908A9B3A" modified="2019-06-05" version="1">MSENHYNNAQKHSYSETTHSIAERFRGYLPVVIDVETGGFNCATDALLEIAATTISMDVKGFLFPKHTNFFRVEPFEDANIETAALEFTGIKLDHPLRMAVSEETALTDIFHDVRKALKTNGCNRAILVGHNSSFDLGFLNAAVARLDMKHNPFHPFSSFDTATLAGLAYGQTVLAKACQAAGIDFDGREAHSALYDTEKTAELFCEIVNRWKQMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMS6</accession>
    <name>A0A481QMS6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrofolate synthase/folylpolyglutamate synthase</fullName>
            <ecNumber evidence="6">6.3.2.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Folylpolyglutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">folC</name>
        <name evidence="15" type="ORF">C3B55_00800</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Functions in two distinct reactions of the de novo folate biosynthetic pathway. Catalyzes the addition of a glutamate residue to dihydropteroate (7,8-dihydropteroate or H2Pte) to form dihydrofolate (7,8-dihydrofolate monoglutamate or H2Pte-Glu). Also catalyzes successive additions of L-glutamate to tetrahydrofolate or 10-formyltetrahydrofolate or 5,10-methylenetetrahydrofolate, leading to folylpolyglutamate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = (6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:51912"/>
            <dbReference type="Rhea" id="RHEA-COMP:13257"/>
            <dbReference type="Rhea" id="RHEA-COMP:13258"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:136572"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>10-formyltetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = 10-formyltetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:51904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13088"/>
            <dbReference type="Rhea" id="RHEA-COMP:14300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:134413"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7,8-dihydropteroate + ATP + L-glutamate = 7,8-dihydrofolate + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00800"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00157"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="PTHR11136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="13" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="14">
        <location>
            <begin position="48"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX82115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="435" mass="48090" checksum="F8AE1A1B1B02D805" modified="2019-06-05" version="1">MIQRKLGEWLAYLEQLHPLVIDMSLERSQQVVARLGLGGQSTPRVITVTGTNGKGSTCAFVASLLQAQGLKVGVYTSPHLLRYNERVHLDGIEATDEQLCEAFAALDVGRREISLTYFEMGTLAAFWLFKRAQLDVVILEVGFGGRLDTVNLMDADLALVTSIGIDHSDYLGNTRESVSYEKAGIFRYGKPVLCGDPDPPQPLLDKVHELDCQFFLRGREFDFNISGKHWQWRGRNVRGQAVELHNLPLLNLPMENAALALQAYSLLDLAWNAGQIAATLQAVRVVGRLDRRVLEWQGRYLSLLLDVGHNQHAAEYLAQRLSLTSLAGRRLAVFGLLADKDLNGVVAPLLDHVQTWAVAPLNTLRCRPATELEIVLQTLGASVESYTSVTAALEAQCMAATAEDEILLFGSFYCVAEALEWLAKRFTQKTDSING</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMV3</accession>
    <name>A0A481QMV3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00827"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="47" mass="5133" checksum="A91149F2EFC794DB" modified="2019-06-05" version="1">MGGYHSLNQDAPSLIDRPDLNKLPGAVFNHAALFFHILLLSLFVVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMV5</accession>
    <name>A0A481QMV5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aspartokinase</fullName>
            <ecNumber evidence="13">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">lysC</name>
        <name evidence="16" type="ORF">C3B55_00891</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 13">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="13"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00891"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04261">
        <property type="entry name" value="AAK_AKii-LysC-BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041740">
        <property type="entry name" value="AKii-LysC-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00656">
        <property type="entry name" value="asp_kin_monofn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="267"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="347"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="7"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="211"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000726-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX82197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="413" mass="44587" checksum="F51CF761CD9F959A" modified="2019-06-05" version="1">MALIVQKFGGTSVGSVKRIAQVADKIKKFQDAGNHLVVVLSAMSGETNRLLNLAKAISGDEQPLPRELDVILSTGEQVTIALLAIALNKRSVPAVSYTGSQVSILTDSAYTKARILRIDDRKIRTDLQAGRVVVVAGFQGMDEQGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYTTDPRVVSAAQRMDKITFEEMLEMASLGSKVLQIRAVEFAGKYSVPLRVLHSFKEGPGTLITIDEEESMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISGANIEVDMIVQNVSHDNTTDFTFTIHRNEYDAAELILQNTASKISAREVIGDIKIAKVSIVGVGMRSHAGVASRMFEVLAKEGINIQMISTSEIKVSVVIEEKYLELAVRTLHTAFKQDAPVQQCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMW5</accession>
    <name>A0A481QMW5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Usp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00717</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00717"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="5"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="63" mass="7135" checksum="E7F66BBE24FBE496" modified="2019-06-05" version="1">MASLRQEINYFTKEQHCNLIVVGSHGRYGLTLLLGSTTNDVLYDAPCYVLAVKLEKHSRLIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMX9</accession>
    <name>A0A481QMX9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00637"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="108" mass="12110" checksum="05C1B57E418231FA" modified="2019-06-05" version="1">MLGCELLLVPLFAIIANQDFNDFVSMSDNIVVLRSVIISSNYELMASFVCTTIQKITDTVSSIERLTILTFIAKVGVNQFFMLALADLHGLRKNEVVGWYLSCEQVCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMZ1</accession>
    <name>A0A481QMZ1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00649"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032608">
        <property type="entry name" value="DUF4892"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16234">
        <property type="entry name" value="DUF4892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019864985" evidence="1">
        <location>
            <begin position="24"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="272" mass="30316" checksum="4EA28E214818694E" modified="2019-06-05" version="1">MSLRKKCLYVVIWLCCLSHSVLAVDVSGSQDLSAVSSQINAKIIDYRYVKEKKRIYPMGAIRKISGQLRYEGQTVARGQIIAITYELPAEHTASAAFTATRKALQTSGAQLLFWCQARDCGESSLWANQVFSNVKLIGADDQQEYLLIRLAAPQDNSLVVLYGITRVNHRAYLHVEQLDACTPLEDLLPTSATLMRELKSTGDLYFPVLAEPVATWLTLISRSLNFDATMRISLVGLTSEAWRQGLIDRGVCASRLHTDSSDIKGLRLHLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN03</accession>
    <name>A0A481QN03_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proline--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="9">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">proS</name>
        <name evidence="11" type="ORF">C3B55_00934</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00934"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00861">
        <property type="entry name" value="ProRS_anticodon_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00779">
        <property type="entry name" value="ProRS_core_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01569">
        <property type="entry name" value="Pro_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004500">
        <property type="entry name" value="Pro-tRNA-synth_IIa_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023717">
        <property type="entry name" value="Pro-tRNA-Synthase_IIa_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044140">
        <property type="entry name" value="ProRS_anticodon_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033730">
        <property type="entry name" value="ProRS_core_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001535">
        <property type="entry name" value="ProRS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="SSF55826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00409">
        <property type="entry name" value="proS_fam_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="10">
        <location>
            <begin position="42"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="575" mass="64545" checksum="9A17AF8D360DEEF2" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN08</accession>
    <name>A0A481QN08_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Thymidylate kinase</fullName>
            <ecNumber evidence="3 6">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">tmk</name>
        <name evidence="8" type="ORF">C3B55_00874</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3 6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00874"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate_kin" evidence="7">
        <location>
            <begin position="8"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="210" mass="23181" checksum="59B958E4C538E153" modified="2019-06-05" version="1">MTGLFITLEGLEGAGKSTNQNYLANRLRAEGIEVVLTREPGGTTLTERMRDVLLAPSAEQMNSDTELLLIFAARAQHLAKVIRPALARGVVVICDRFTDSTYAYQGGGRGLPLERIATLESFVQGDLRPDLTMLFDLPIEVGIARTSVYGRLDRFELENRAFFDAVRMVFLELAKAKPARYSLIDAAQPLTQVQQAIDALLPVCLERVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN22</accession>
    <name>A0A481QN22_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Malonyl CoA-acyl carrier protein transacylase</fullName>
            <ecNumber evidence="2 5">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fabD</name>
        <name evidence="8" type="ORF">C3B55_00879</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the fabD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="2 5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00879"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024925">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004410">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc_FabD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000446">
        <property type="entry name" value="Mct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="SSF55048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00128">
        <property type="entry name" value="fabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_AT" evidence="7">
        <location>
            <begin position="8"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000446-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="312" mass="33302" checksum="182AF3DA26A57231" modified="2019-06-05" version="1">MSATFAFVFPGQGSQSIGMLAELSAQYPLIQETFKEASDVLGYDMWSLTQHGPKKYLSQTDKTQPAILTASIALWRLWLSKGGERPAYVAGHSLGEYSALVATDSVSFVEAVKLVERRGQLMQEAVPAGHGGMAAILGLDNIVVIEVCAEAAHDEVVSVANFNSPGHVVISGTKAAVVRAIEGCKARGAKLTLPLLVSVPSHCKLMRPAAERFAESIFAINWQTPQIPLVQNVSATFAADLNTLQCDLLRQLYKPVRWVELIQTLVANSVTKLVECGPGKVLTGLNKRCADGVSVANLNTLDAFAAARAALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN29</accession>
    <name>A0A481QN29_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00886</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00886"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="45" mass="5130" checksum="675F0D022995740C" modified="2019-06-05" version="1">MRKSLVSSMSNPDLDFDLCVDTRAKHHNHAIIARMHLNEGSRLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN42</accession>
    <name>A0A481QN42_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">minD_1</name>
        <name evidence="7" type="ORF">C3B55_00781</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the MIP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00781"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="PTHR42961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="6">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="349" mass="37457" checksum="AC2A9944138549C7" modified="2019-06-05" version="1">MKAVNRAAIEAVLRQYTDPYLNLDLVSSGCVRAIDVNNDQVLVKLEFGYAAGLFKSDLAQMLQMTIEGLAGVRLAKVETRCVITSRKTQVQLHGLANVKNIIAVASCKGGVGKSTTAANLALALDREGACVGILDADIYGPSQGVMFGVPDGTRPKVKDQKWFVPIKSLGIEVMSMAFLAEDSAPMIWRGPMVSSALLQLVSQTAWGDLDYLVIDMPPGTGDIQLTLAQKVPVVGLVIVTTPQDLALRDAKKSMEMFRKVNIPVLGVVENMAVHICSSCGYAEHLFGEGGGEKLATQYSIELLASLPLSIIIREQADSGKPIVAAEPDSQIAMVYQELARHVSARISMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN49</accession>
    <name>A0A481QN49_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="7">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="7">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="7">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">aspS</name>
        <name evidence="9" type="ORF">C3B55_00917</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00917"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="PTHR22594:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="141"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="7">
        <location>
            <begin position="198"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="535"/>
            <end position="538"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="7">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="7">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="591" mass="66757" checksum="462826A42D16917B" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNA0</accession>
    <name>A0A481QNA0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2Fe-2S ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fdx_2</name>
        <name evidence="5" type="ORF">C3B55_00959</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00959"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011536">
        <property type="entry name" value="Fdx_isc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="PTHR23426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02007">
        <property type="entry name" value="fdx_isc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="113" mass="12296" checksum="AF35AB2AD7E73C1E" modified="2019-06-05" version="1">MPQVIFLPHAKHCPDGMVVEAETGQSILEVAHDNHIELESACGGVCACTTCHCVIREGFQSLEEADELEENLLDCVWGLEITSRLSCQAKIGLGDIAVEIPKYSLNHAAEILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNC5</accession>
    <name>A0A481QNC5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00981</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00981"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="52" mass="6018" checksum="B66448E839175097" modified="2019-06-05" version="1">MSLSYSASTPLICLYAANISAFQRQQHTEYCLLSLKQFYHKLITLLVDCYRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNE1</accession>
    <name>A0A481QNE1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetylmuramoyl-L-alanine amidase AmiD</fullName>
            <ecNumber evidence="2">3.5.1.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">amiD</name>
        <name evidence="2" type="ORF">C3B55_00991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.28" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00991"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="SSF55846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="262" mass="29444" checksum="7F597027D5A99500" modified="2019-06-05" version="1">MPFVMKYLQSIVVFFLLAGCADSLHLNTKYSSSSHDNRVQFVIVHYTTASLERSITLLTHGPVSSHYLISDDASGTIYKLVDESQRAWHAGESEWMGRTWLNSSSIGIEIVNPGYKDMSTGRVWYPYFESQMQSLIILLKDISNRNGIDPKNIIGHSDIAPLRKLDPGPLFPWKRLAVEDLGVWPDIQVVTKFQKQYAAGLPSITWFQEELARLGYQTPQTGELDTATRHVIAAFQMHFRPSLFDGMPDAESAAILHALSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNH4</accession>
    <name>A0A481QNH4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secF</name>
        <name evidence="10" type="ORF">C3B55_00968</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecD. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00968"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_B">
        <property type="entry name" value="SecF_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005665">
        <property type="entry name" value="SecF_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01755">
        <property type="entry name" value="SECFTRNLCASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
        <property type="entry name" value="2A0604s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00966">
        <property type="entry name" value="3a0501s07"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="255"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="291" mass="32044" checksum="EC618FC730CFB458" modified="2019-06-05" version="1">MLRTINFMGVRNIACSATLLITIFALFSWFYKGLNYGLDFTGGTLIELTYEKPTDIAMVRSELVKAGYHEVIVQSFGVTTDLLVRMSGGDPQLGHEIAKILQKISSNNPASVKRVEFVGPQVGADLREQGGLAMLMTLVSIMIYLAFRFQWKFGVGAVVSLIHDVIVTIGILTYFQITFDLTVLAAVLAIIGYSLNDTIIIFDRVRENFRILRKASLIENLNISTTQTLLRTIATSISTLLAIAALMIFGGDNLWGFSLALFVGVLAGTYSSIYIASLVLIWLHLNSEDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNL5</accession>
    <name>A0A481QNL5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fumarate hydratase class I</fullName>
            <ecNumber evidence="10">4.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fumA</name>
        <name evidence="13" type="ORF">C3B55_00926</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible hydration of fumarate to (S)-malate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(S)-malate = fumarate + H2O</text>
            <dbReference type="Rhea" id="RHEA:12460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="EC" id="4.2.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00926"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004647">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdB-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036660">
        <property type="entry name" value="Fe-S_hydroAse_TtdB_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011167">
        <property type="entry name" value="Fe_dep_fumarate_hydratase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05683">
        <property type="entry name" value="Fumerase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001394">
        <property type="entry name" value="Fe_dep_fumar_hy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117457">
        <property type="entry name" value="SSF117457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00723">
        <property type="entry name" value="ttdB_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 10 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase" evidence="11">
        <location>
            <begin position="11"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Fumerase_C" evidence="12">
        <location>
            <begin position="289"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX82229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="504" mass="55127" checksum="9C5D223101131485" modified="2019-06-05" version="1">MTMIKQEDLIQSVADTLQFISYYHPMDFIRAMHEAYLHEESLIARNAIAQILINSRMCAIGHRPICQDTGIVNVFLRIGMNVHWDSTTMSLNDMINEGVRRAYNLPENILRASVLADPAGTRKNTKDNTPAVIHYSIIPGDTIEVDVAAKGGGSENKSRMAMLNPSDSIVDWVLKTVPTMGAGWCPPGILGIGIGGTAEKAAVMAKEVLMESIDIHELKKRGPSNYIEEMRLELFKKVNQLGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHARFVLDGSGSASLEAPSLDSYPEIVWKTCPVARYVNLNTLTHEQVQSWKPGETVLLNGKMFTGRDAAHKRIVEMLNKGEVPPIDLKDRFIYYVGPVDPVREEVVGPAGPTTATRMDRFTRQLLEQTGLLGMIGKAERGKTAIEAIKDYKAVYLIAVGGAAYLVAQAIKKSRVVAFTELGMEAIYELDVTNMPATVAVDSKGESIHITGPAIWQKKISESLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNP9</accession>
    <name>A0A481QNP9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative membrane protein insertion efficiency factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yidD</name>
        <name evidence="3" type="ORF">C3B55_01020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in insertion of integral membrane proteins into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0161 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01020"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00386">
        <property type="entry name" value="UPF0161_YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002696">
        <property type="entry name" value="Membr_insert_effic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33383">
        <property type="entry name" value="PTHR33383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01809">
        <property type="entry name" value="YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01234">
        <property type="entry name" value="Haemolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00278">
        <property type="entry name" value="TIGR00278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="85" mass="9909" checksum="438BA28A16C53224" modified="2019-06-05" version="1">MLQMYKLALILIQFYCYVISPIMVNHCRFYPTCSFYALEAINNHGFLRGCWLTFCRLSRCHPYNPGGFDPVPPILISCSFLITTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNR2</accession>
    <name>A0A481QNR2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00833"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="38" mass="4225" checksum="881010CC3DC36E21" modified="2019-06-05" version="1">MKQPLIYVVSCLRKSAKLIDVNIVIQMLAVAQPKKATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNT6</accession>
    <name>A0A481QNT6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin reductase</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">C3B55_00980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00980"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="5">
        <location>
            <begin position="8"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="320" mass="34352" checksum="12F0CD74712EF984" modified="2019-06-05" version="1">MSDTRHSRVIILGSGPAGYSAAVYAARANLQPLLITGTQVGGQLTTTTEIDNWPGDVYGLTGPALMGRMKEHAERFKTEIVFDHIHKVNFSNQPYKLIGDISVYTCDALIITTGASASYLGLPSEEAFMGKGVSACATCDGFFYRNKPVAVVGGGNTAVEEALYLANIASTVTLVHRRETFRTEKLLRDKLYARVSEGKIILKLNATLNEVLGDSIGVTGIRLKNNHGSYDELKVDGVFIAIGHTPNTSLFEGILDIKDGYLVVQGGFQGNATQTNIKGIFAAGDVADHVYRQAITSAATGCMAALDAERYLDSLNNAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNX0</accession>
    <name>A0A481QNX0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endolytic murein transglycosylase</fullName>
            <ecNumber evidence="7">4.2.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Peptidoglycan polymerization terminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mltG</name>
        <name evidence="8" type="ORF">C3B55_00875</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transglycosylase MltG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.-" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00875"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008932">
        <property type="term" value="F:lytic endotransglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08010">
        <property type="entry name" value="MltG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02065">
        <property type="entry name" value="MltG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003770">
        <property type="entry name" value="MLTG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30518">
        <property type="entry name" value="PTHR30518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02618">
        <property type="entry name" value="YceG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00247">
        <property type="entry name" value="TIGR00247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="377" mass="42209" checksum="C4DBCAC08C8ADFB2" modified="2019-06-05" version="1">MLKLTRKIVLLLQISLVSAGLLSCLAAWKFDAILKQPLNLTQVKLLNVSVGATPTGTFNHLKNDGLLDSPFWLSLYWRLNLANQPLQCGEYRMIPGMTAKGLISLWQRGEVVQYSLTLVEGWNFNRVRLALVKHEKIVQTLSGLSNSDVMEKLGHPGVFPEGRFFPDTYRFVRGMTDVEFLKKAYNRLDYILAQEWSKRAADASYADSYQALIMATLIEKETSVPEERGQIAGVFMRRMKIGMLLQADPTVIYGLGERYTGRLTRVHLKEANPYNTYMVAGLPPTPIAMAGRKSIHAALNYVPSNHLYFVARGDGSHIFSDDLNSHNAAVREFQLKRCTYYRSSPAPTVKSMQDTILSADSAPAKQSTNFVPNTATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNY8</accession>
    <name>A0A481QNY8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Enterobactin synthase component D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">4'-phosphopantetheinyl transferase EntD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Enterochelin synthase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">npt</name>
        <name evidence="14" type="ORF">C3B55_00885</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron(2+) atoms. Plays an essential role in the assembly of the enterobactin by catalyzing the transfer of the 4'-phosphopantetheine (Ppant) moiety from coenzyme A to the apo-domains of both EntB (ArCP domain) and EntF (PCP domain) to yield their holo-forms which make them competent for the activation of 2,3-dihydroxybenzoate (DHB) and L-serine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>apo-[aryl-carrier protein] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-[aryl-carrier protein]</text>
            <dbReference type="Rhea" id="RHEA:48404"/>
            <dbReference type="Rhea" id="RHEA-COMP:15903"/>
            <dbReference type="Rhea" id="RHEA-COMP:17557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>apo-[peptidyl-carrier protein] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-[peptidyl-carrier protein]</text>
            <dbReference type="Rhea" id="RHEA:46228"/>
            <dbReference type="Rhea" id="RHEA-COMP:11479"/>
            <dbReference type="Rhea" id="RHEA-COMP:11480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Siderophore biosynthesis; enterobactin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the P-Pant transferase superfamily. EntD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00885"/>
    <dbReference type="UniPathway" id="UPA00017"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009366">
        <property type="term" value="C:enterobactin synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009239">
        <property type="term" value="P:enterobactin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041354">
        <property type="entry name" value="4PPT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003542">
        <property type="entry name" value="Enbac_synth_compD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38096">
        <property type="entry name" value="PTHR38096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17837">
        <property type="entry name" value="4PPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01399">
        <property type="entry name" value="ENTSNTHTASED"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0259">Enterobactin biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4PPT_N" evidence="13">
        <location>
            <begin position="52"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="ACPS" evidence="12">
        <location>
            <begin position="121"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX82194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="238" mass="26407" checksum="95958F411E314A22" modified="2019-06-05" version="1">MTPLPACCTPLNAHWPLPIQVPGTVLLSTIFDSTLLIDGDFQRSAVPLPVSIQRSIVKRQAEFLIGRLCARAALQKLDQLDYVPALGKDRAPVWPEHISGSITHNTGHAAAIVGHKTQWCGLGVDLENVLTINRAECLAEEILTTNELLRMATLPHEQIALLVTMTFSVKESLFKALYPIVQKHFYFEHAEVLKWSQTGLVQLQLLKDLSTTWFYGRKLEGQFAISRGQLFSLVAIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QP03</accession>
    <name>A0A481QP03_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lysine-arginine-ornithine-binding periplasmic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">argT</name>
        <name evidence="6" type="ORF">C3B55_00899</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00899"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071705">
        <property type="term" value="P:nitrogen compound transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005768">
        <property type="entry name" value="Lys_Arg_Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01096">
        <property type="entry name" value="3A0103s03R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019764897" evidence="4">
        <location>
            <begin position="19"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="5">
        <location>
            <begin position="22"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="258" mass="28604" checksum="D8E77845E32173AD" modified="2019-06-05" version="1">MKKLVLLSAIVLSMQVIAVETPVKIGIEAAYPPFSSKAPDGSIIGFDYDIGNALYAEMKVKCVWIEQEFDSLIPALKVRKIDAILSSMSITEDRKKSVDFTIRYYRTYARLVMKEATTVSDSLHELKGKKIGVQRGSIHNRFAKEVLAAKGAVVVPYSSQNEIYLDLEIGRLDGTIADVTLLKDGFLKTNAGKGYAFVGPRFTDVKYFGDGVGIAVRKGDKATLDKINSAIEAILANGEYKKIKDKYFDFDIYSFDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-08-12" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK27</accession>
    <name>A0A481QK27_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Porin-like protein NicP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nicP</name>
        <name evidence="3" type="ORF">C3B55_00057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane porin (Opr) (TC 1.B.25) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00057"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005318">
        <property type="entry name" value="OM_porin_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34596">
        <property type="entry name" value="PTHR34596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03573">
        <property type="entry name" value="OprD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019798982" evidence="2">
        <location>
            <begin position="22"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="444" mass="49110" checksum="DC3730096F2F3F73" modified="2019-06-05" version="1">MNKLIFIAAITFAVIAQQVNSAEFIEDSQATLSLRNFYINTNNYNSSLTLNSHSKNFEWGQGLDLRFMSGFTQGTLQFGIDAIGLYGLRLNSSHINYGSTKSRSTVFPSNGNQAVNDFSSLGVTGKIKISKTVAKIGTLQPNNPVIKINDGRLLPQTFQGSEIMSNEITNLTLTAGQIESVKGRNSSNNENLSVISVNKNLHYNTAKFSNKFYYAGAYYKITKDLTASYYFGELKKFYSQNFLGVVHNLAIGSGVLKSDLRYYRSRDNGAYTNTVNCYTSCYYTDGAKPVTNSKVDNDLYSYLGVYSMSGHTFGGGYQYINGSSDFMWLNQGDGSSNSTTTDMQIQKFASAGERTWQVRYAYDFAKVGVHGMTASIVYLKGTSIQMLAGNKSEWERDIIITYVIPNGPLKNFGVAWKNAMWHSGVAGHTQGENRVTASYSVPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK72</accession>
    <name>A0A481QK72_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-octaprenyl-4-hydroxybenzoate carboxy-lyase</fullName>
            <ecNumber evidence="1">4.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiD</name>
        <name evidence="1" type="ORF">C3B55_00051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00051"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="PTHR30108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00148">
        <property type="entry name" value="TIGR00148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="491" mass="55498" checksum="03C6A9057E2DE553" modified="2019-06-05" version="1">MKFKDLRDFVRQLEQRRELKRIQVPISPVLEMTEICSRVLHNKGPALLFENPTGFDIPVLGNLFGMPKRVAFGMGAESLNELREIGKLLSFLKEPEPPTGIKDAWSKLPIFRKIIAMAPKVVKDAACQEVVIEGDNVDLAMLPVQTCWPGDVGPLITWSLTITKGPHKDRQNLGIYRQQVIGRNKLIMRWLSHRGGALDFREWCEKYHDQPFPVSVAIGADPGTILSAVTPVPDSLSEYAFAGLLRGSRTELVKCRSNNLQVPATAEIILEGVIHPGEMAEEGPYGDHTGYYNEVHSFPVFTVACITHRMQPIYHSTYTGRPPDEPAILGMALNEIFVPILQKRFPEIIDFYLPPEGCSYRIAIVTMKKSYPGHAKRVMFGVWSFLRQFMYTKFIIITDEDINARDWSDVIWAITTRMDPKRDTVIINNTPIDYLDFASPISSLGSKIGMDATNKWPGETSRKWGQVIIKDKAVTARIDTIWQELGINEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK78</accession>
    <name>A0A481QK78_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0301 protein C3B55_00091</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0301 (AlgH) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00091"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00758">
        <property type="entry name" value="UPF0301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003774">
        <property type="entry name" value="UPF0301"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30327">
        <property type="entry name" value="PTHR30327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02622">
        <property type="entry name" value="DUF179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="191" mass="20740" checksum="89700E11B3991F00" modified="2019-06-05" version="1">MDMKNASSTYLKHQFLIAMPHMTDPNFTQTLTYVVEHTTNGAMGLVVNRPQELSLADILEQLRPEIDPPISCQNVPIYIGGPVQVDRGFVLHLTGPKFQATIDLEDMSLSTSQDVLFAIADGVGPKQSVITIGYAGWEAGQLEDELASNIWLTCPFDADILFNTASELRLKAAAAKLGVDLSLLTNQAGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKA8</accession>
    <name>A0A481QKA8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase subunit alpha</fullName>
            <ecNumber evidence="14">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP synthase F1 sector subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">F-ATPase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 18" type="primary">atpA</name>
        <name evidence="18" type="ORF">C3B55_00009</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has four main subunits: a(1), b(1), b'(1) and c(9-12).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ATPase alpha/beta chains family. T3SS ATPase subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="14"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00009"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1_ATPase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 14" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5 14" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_ab_N" evidence="17">
        <location>
            <begin position="30"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab" evidence="15">
        <location>
            <begin position="150"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab_C" evidence="16">
        <location>
            <begin position="383"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="170"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Required for activity" evidence="14">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="QAX81384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="514" mass="55656" checksum="46F6E67D3B5C1554" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKD4</accession>
    <name>A0A481QKD4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00095"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="37" mass="4116" checksum="E0504A4E39535ACC" modified="2019-06-05" version="1">MSKVVLFYGSEHIAVCGIRRLAACDLDPFYVLTKLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKG3</accession>
    <name>A0A481QKG3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rpmB</name>
        <name evidence="7" type="ORF">C3B55_00033</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00033"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00373">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribo_L28/L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribo_L28/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001383">
        <property type="entry name" value="Ribosomal_L28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528">
        <property type="entry name" value="PTHR13528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00009">
        <property type="entry name" value="L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="77" mass="8853" checksum="581984EC3890E65D" modified="2019-06-05" version="1">MSRVCQVTGKSPVTGNNISHANNKTRRRFLPNLQRHRFWVEEEKRFVRLRVSVKAIRIIDKLGIKAVLAAIRTVGNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKM2</accession>
    <name>A0A481QKM2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">ATP synthase epsilon chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">ATP synthase F1 sector epsilon subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 13">F-ATPase epsilon subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 16" type="primary">atpC</name>
        <name evidence="16" type="ORF">C3B55_00012</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13 14">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13 14">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00012"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036794">
        <property type="entry name" value="ATP_F1_dsu/esu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="PTHR13822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46604">
        <property type="entry name" value="SSF46604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="SSF51344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01216">
        <property type="entry name" value="ATP_synt_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13 14" id="KW-0139">CF(1)</keyword>
    <keyword evidence="13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 13 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_DE_N" evidence="15">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX81387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="136" mass="14724" checksum="AFDD63B9BD9ED17C" modified="2019-06-05" version="1">MMRIHCDIVSAEAEIFSGFVEMVIARGKLGALGITHGHTPLITSLKPGSITLTKKGGEKEVFYVSGGFLEVQPHMVKVLADTAQRAGDIDEVFAKEAIKAAKKALNKNSSDFDYSAALRLTEAAAQLHTLQQIRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKN7</accession>
    <name>A0A481QKN7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-dehydroquinate synthase</fullName>
            <shortName evidence="11">DHQS</shortName>
            <ecNumber evidence="11">4.2.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">aroB</name>
        <name evidence="13" type="ORF">C3B55_00187</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 divalent metal cation per subunit. Can use either Co(2+) or Zn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00187"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00110">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030963">
        <property type="entry name" value="DHQ_synth_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001455">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0170">Cobalt</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DHQ_synthase" evidence="12">
        <location>
            <begin position="65"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="69"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="103"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="127"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="167"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="366" mass="40153" checksum="514E6F98819E41E0" modified="2019-06-05" version="1">MQTLKVNLGERSYPIHIGEGLLDLPELFAPHIIGRQVAIVSNKIVASLYLERLSHSLAAYSVISVILPDGEINKNWKTMQLIFDALLTARHDRYTTVVALGGGVIGDMAGFAAACYQRGVGLIQVPTTLLSQVDSSVGGKTGINHQLGKNMVGAFYQPQAVLIDTTTLNTLPSRELSAGLAEVIKYGLICDKSFLVWLEKYIDDIRALSQVALTEAISRSNTAKAFVVNADERESGVRATLNLGHTFGHAIETYMGYGTWLHGEAVAVGTVIALEMSQRMGWISIQERNYGIHLFLRAGLPVIPPAEMTEADFLERMIIDKKVIDGRLRLVLLRHIGEAVLTDDYPKEILQTTLRANYNALVHCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKP2</accession>
    <name>A0A481QKP2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7,8-dihydroneopterin aldolase</fullName>
            <ecNumber evidence="1">4.1.2.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">folB</name>
        <name evidence="3" type="ORF">C3B55_00114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DHNA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00114"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00154"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00534">
        <property type="entry name" value="DHNA_DHNTPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006156">
        <property type="entry name" value="Dihydroneopterin_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844">
        <property type="entry name" value="PTHR42844"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00525">
        <property type="entry name" value="folB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00526">
        <property type="entry name" value="folB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="1 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FolB" evidence="2">
        <location>
            <begin position="4"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="118" mass="13622" checksum="1E0D7663DD3A7C0C" modified="2019-06-05" version="1">MDRLFIEGLEVDTVIGAYDWERSIKQCLRLDLSVAWDNRQAAANDDLALVLDYTSVCTRIQVFAEKSQYQLLETFAEQLAEMLMSEFQIPWLRLKLIKPGVIPTINSVVGVEIERRCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKP9</accession>
    <name>A0A481QKP9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="4">OPRT</shortName>
            <shortName evidence="4">OPRTase</shortName>
            <ecNumber evidence="2 4">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">pyrE</name>
        <name evidence="6" type="ORF">C3B55_00030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00030"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="54"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Orotate binding" evidence="4">
        <location>
            <begin position="34"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="72"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="124"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="214" mass="23514" checksum="E740B1851FFD11B3" modified="2019-06-05" version="1">MQVYQRHFIRFAVSCGVLRFGKFTLKSGRISPYFFNTGLFNSGLVLARLGSFYAAAIVNQCISFNVLFGLAYKGIPLAAATAMSLAEHYALDLPLCFNRKEFKVHGEGGNLVGATLKGNILIIDDVITAGTAIREAIQIISSQDGAKVAGVLIALNRQERGNGELSAIQEVERDFHIPVISIISLNQVLQYLEDDSQLNQHLHAVRAYHEQFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKQ1</accession>
    <name>A0A481QKQ1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-Glc:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,3-glucosyltransferase WfgD</fullName>
            <ecNumber evidence="3">2.4.1.305</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">wfgD_1</name>
        <name evidence="3" type="ORF">C3B55_00208</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.305" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00208"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="9"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="295" mass="34458" checksum="E0732A958A2FDF93" modified="2019-06-05" version="1">MSYSQPLVTVIIASYNHAPYIEESILSVLNQTYLNIELLVIDDGSTDNSGERIRYLQESHTFDFHVQHNHGLTNTLNSAISRSHGSLVVPFSSDDIMMPERIAIQVDYMNDKPEVGICAGNIELIDANGKLFQEKRQRRNVSFRRMDFDDIFLERKPYPPAPTLMIRREALEKVNRFDPQIFLEDLLIELKITNAGYFIDSLNIVMARYRKHATNSYKNHRFMIDNILRSYALFSDHPQYDKVRYKFLNSMFLKTSNRNCALARELLAQIPFLAWNKKTWRGLSRLLFSPLEKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKR5</accession>
    <name>A0A481QKR5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HIT-like protein</fullName>
            <ecNumber evidence="3">3.-.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.-.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00128"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="PTHR23089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="4"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="112" mass="12705" checksum="7C1CD61862CFA80F" modified="2019-06-05" version="1">MDTLFTKIINKEIPAKIFYEDSQVLAFHDTAPMAPVHFLVIPKKPIRNLNELTEGDKVLAGHILFIAQRLALEQGCEKGFRVVVNCNEDGGQTIYHIHMHVLGKRQMNWPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKS3</accession>
    <name>A0A481QKS3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-binding protein Hfq</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">hfq</name>
        <name evidence="2" type="ORF">C3B55_00229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Hfq family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00229"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01716">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00436">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005001">
        <property type="entry name" value="Hfq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34772">
        <property type="entry name" value="PTHR34772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17209">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02383">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="86" mass="9657" checksum="C9D8208358E45158" modified="2019-06-05" version="1">MSKRHSLQDPYLRALLKEKVGVSIYLVNGIKLQGTIESFDQFVILLKNTVSQMVYKHAISTVVPVRQIQLLNIAGDEQSDAELSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-04-07" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKS4</accession>
    <name>A0A481QKS4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulatory protein OmpR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ompR</name>
        <name evidence="6" type="ORF">C3B55_00146</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00146"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="12"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="141"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="141"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="246" mass="27792" checksum="FE2DCDA145BD101D" modified="2019-06-05" version="1">MNSTTQTVEGKKILFVDDDLGFSNLLERFFNTKGYRARAVPNTEQMDRLLGREVFNLVILDLILPGEDGLTACKRLRGINNQIPIIMLTARSDEWSRIKGLELGADDYLTKPFNPDELMARVKAVLRRQVALVPGAPGNEDASVTFGSYELSLATRELKCSKEVYMLTTGEFAVLKALVMNARQPLTRDKLMNLARGREWDALERSIDVQISRLRRIIELDPSKPRYIQTVWGVGYVFVPDGTVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKT5</accession>
    <name>A0A481QKT5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">50S ribosomal protein L9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">rplI</name>
        <name evidence="10" type="ORF">C3B55_00240</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00240"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.430.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00503">
        <property type="entry name" value="Ribosomal_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_L9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_L9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020594">
        <property type="entry name" value="Ribosomal_L9_bac/chp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020069">
        <property type="entry name" value="Ribosomal_L9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036791">
        <property type="entry name" value="Ribosomal_L9_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_L9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_L9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="PTHR21368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03948">
        <property type="entry name" value="Ribosomal_L9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55653">
        <property type="entry name" value="SSF55653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="SSF55658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00158">
        <property type="entry name" value="L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RIBOSOMAL_L9" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L9_C" evidence="9">
        <location>
            <begin position="60"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="144" mass="15364" checksum="6CF850465FA10D88" modified="2019-06-05" version="1">MQLILLEKIANLGNLGDKVKVKAGYSRNYLLPYGKATADLVAFEERRVELEKAAADKKSSAKNRAAQLAELEVTITATAGAEGKLFGSIGTHDIADALTASGVEVQKREIRLPSGTIRNLGEFNVAVHLHAEVEAIVRVVVVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKU5</accession>
    <name>A0A481QKU5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00202"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="29" mass="3560" checksum="EF84FFAB549617F0" modified="2019-06-05" version="1">MPRILMGLERPRQLLFGKRNYIKDLKLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKW6</accession>
    <name>A0A481QKW6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00172"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="36" mass="4250" checksum="766351C3E1012183" modified="2019-06-05" version="1">MMQYTLYEFIKNINYCALRLLALLDKSPTVTNRLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKY2</accession>
    <name>A0A481QKY2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">rpsQ</name>
        <name evidence="4" type="ORF">C3B55_00286</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00286"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_B">
        <property type="entry name" value="Ribosomal_S17_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019984">
        <property type="entry name" value="Ribosomal_S17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03635">
        <property type="entry name" value="uS17_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="88" mass="10048" checksum="8CEA3AAC1A355785" modified="2019-06-05" version="1">MAEAKKTIRTLTGRVISDKMDKTITILIERRVKHAIYGKYVKRSTKLHAHDETNQCHVGDKVTICETRPLAKTKSWKLVNVLECVAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKY3</accession>
    <name>A0A481QKY3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">gcvH</name>
        <name evidence="6" type="ORF">C3B55_00055</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="3">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00055"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="PTHR11715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="3 4">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="127" mass="13832" checksum="D186D54FF5658ED7" modified="2019-06-05" version="1">MSNNFAKLRFTKSHEWALLEANGTVIVGISDHAQASLGDVVFVELPEIGVNCDTKSQAGVVESVKSASDIYAPIAGEVVAVNEELSISPELLNSDPYNTWIFRIKPSNLADLEKLLDIVSYQAIINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL07</accession>
    <name>A0A481QL07_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00215"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="39" mass="4571" checksum="C51F0700AA8930FB" modified="2019-06-05" version="1">MAGKTTLDHDVSRAEIFDQQIIYITHHHHLPNHLLRAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL19</accession>
    <name>A0A481QL19_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Heme chaperone HemW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hemN</name>
        <name evidence="12" type="ORF">C3B55_00082</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00082"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010723">
        <property type="entry name" value="HemN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004559">
        <property type="entry name" value="HemW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="PTHR13932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06969">
        <property type="entry name" value="HemN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00562">
        <property type="entry name" value="HemN-like__clustered_with_heat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00539">
        <property type="entry name" value="hemN_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="27"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="405" mass="45656" checksum="985CE571A2A7D87B" modified="2019-06-05" version="1">MPKIKMTKNTASQPIASGGIKMPWAALPLLPPLALYIHIPWCVRKCPYCDFNSHTAHNVLPQEEYVNALLDDFAQDLNTVYNRKLTSIFFGGGTPSLFSAAALSYILKNIEAHIQFADEIEITLEVNPSTVEQENFVSYRKLGINRLSIGIQSFQKNKLQALGRIHDADEALRAVNMAHKAGFNNFNLDLIYGLPGQSLGDALRDLRQAIILKPTHLSWYQLTIEPNTIFWSQPPTLPEENTLWEIQKAGQSLLAKNGYTQYEVSAYAQSGYQAQHNLNYWNFGDFIGIGAGAHSKLSHPDGRIVRTWKTRMPNDYMNLDKTFQAGTKELTKEELPFEFLMNALRLTEGVDANLYTQRTNLDLDSLNKGRSQAEYSGLMQIKPSQLAATNRGQLFLNDLLQKFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL26</accession>
    <name>A0A481QL26_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidoglycan glycosyltransferase MrdB</fullName>
            <shortName evidence="9">PGT</shortName>
            <ecNumber evidence="9">2.4.1.129</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cell elongation protein RodA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Cell wall polymerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Peptidoglycan polymerase</fullName>
            <shortName evidence="9">PG polymerase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">mrdB</name>
        <name evidence="9" type="synonym">rodA</name>
        <name evidence="10" type="ORF">C3B55_00326</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Peptidoglycan polymerase that is essential for cell wall elongation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SEDS family. MrdB/RodA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00326"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02079">
        <property type="entry name" value="PGT_RodA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018365">
        <property type="entry name" value="Cell_cycle_FtsW-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011923">
        <property type="entry name" value="RodA/MrdB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474:SF1">
        <property type="entry name" value="PTHR30474:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02210">
        <property type="entry name" value="rodA_shape"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00428">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="381" mass="42315" checksum="D9424405EBB3BC45" modified="2019-06-05" version="1">MKSNFDRILSSEDLMRRRATLLQRMHIDGPLLILLLTLAAISLFVLYSASGKNWNLLIKQALSFGLGLLSMVVIAQLEPRFMARWVPLGYIIGVFLLVMVDVMGYRTMGATRWINIPGIIRFQPSEFMKILMPAIIAWYLSDRTLPPQLKHVGISLILISVPFTLILCQPDLGTSLLIFTGGVFVLFMGGLRWCWILSVLAVSILVAIVMWFFFMHEYQKQRILTFLNPESDPLGTGWNIIQSKVAIGSGGVFGKGWLLGTQSHLNFLPESHTDFIIAVMGEEFGFFGICTLLLIYLLLISRGLVITAKAQTLFGKLLAGSLTMTFFVYVFVNIGMVSGLLPVVGVPLPLISYGGTSLVTLLSAFGILMSIHTHRKLIVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL30</accession>
    <name>A0A481QL30_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Magnesium and cobalt efflux protein CorC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">corC_2</name>
        <name evidence="5" type="ORF">C3B55_00337</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00337"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="67"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="133"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="279" mass="31403" checksum="541E25FA9FF9866F" modified="2019-06-05" version="1">MSEDRSSNEQKSWLGKLTQVFAHEPKNRQELLGLLREAHQNKLLDSEALAIVEGAIQVADLQVRDIMVPRSQMISIKATQTPREFLPAVIDSAHSRYPVTSESHDDVIGILLAKDLLPLILKENGDNVRIKNLLRPATFIPESKRLNVLLREFRANHNHMAIVIDEYGGVAGLVTIEDVLEQIVGDIEDEHDVEDDSYIKPLPSGDFLVKALTPIENFNEFFGSEFSDNEFYTVGGLVMSAFGHLPKRNETTEIGAYRFRILNADSRRVHLIRLTPVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL31</accession>
    <name>A0A481QL31_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Adenylosuccinate synthetase</fullName>
            <shortName evidence="8">AMPSase</shortName>
            <shortName evidence="8">AdSS</shortName>
            <ecNumber evidence="8 10">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">purA</name>
        <name evidence="11" type="ORF">C3B55_00233</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="8 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00233"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="IMP binding" evidence="8">
        <location>
            <begin position="14"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="8">
        <location>
            <begin position="39"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="300"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="13"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="41"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="332"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="413"/>
            <end position="415"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="429" mass="46779" checksum="86A7BA2EA9CBB8A3" modified="2019-06-05" version="1">MGKNVVVLGTQWGDEGKGKIVDLLTEHTAAVVRYQGGHNAGHTLVVDGKKTVLHLIPSGILREGVQCLIGNGVVVAPDALLREIIRLEEKNVSVRERLRINPSCPLILSFHVALDRAREKARGALKIGTTGCGIGPAYEDKVARRGMRVGDLLNISSFEDKLRELTDYHNFMLVSYYKEQAIEFAKILATCNEYAEMLKPLMLDVTAELHDLRRAGKNIMFEGAQGSLLDIDHGTYPYVTSSNTTAGSVATGSGIGPMFLDYILGVTKAYTTRVGSGPFPTELSNEVGAHLAKQGHEFGATTGRVRRCGWFDAAILRRAIDINSISGVCLTKLDVLDGLETINICVSYKDAKGKNFVPTDADSYAGLYPVYKTVPGWIESTVGVKTLEDLPINARAYIRRIEELIGVPIDIISTGPDRNETIVLRHPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL45</accession>
    <name>A0A481QL45_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="10">D-erythrose-4-phosphate dehydrogenase</fullName>
            <ecNumber evidence="10">1.2.1.72</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">epd</name>
        <name evidence="10" type="ORF">C3B55_00102</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.72" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00102"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048001">
        <property type="term" value="F:erythrose-4-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006422">
        <property type="entry name" value="E4P_DH_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="PTHR43148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01532">
        <property type="entry name" value="E4PD_g-proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="9">
        <location>
            <begin position="7"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="16"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="7">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="347" mass="38018" checksum="A6A455A03BEC1D11" modified="2019-06-05" version="1">MLKLRPYKVALNGYGRIGRCVLRALFERGAAARFEIVAINDLADMASIAYLTSFDSTHGRFPGTVLVDGNFLYINGDCIKVLCSATPEGIGWASLGIDLVLECSGAYYTRTDGQRFLNAGAPRVLFSQPMASETDVDATIVYGINHDHLTGNELLVSNASCTTNCSVPLLRLLNQAIGLDYVLITTIHSAMNDQPVIDAYHHDDLRRTRSAFQSVIPVSTGLARGIERLLPELAGRIQAKAMRVPTVNVSCLDITMQTINDTDVSEVAKILRYAAETGPLKGLLAYTELPHASCDFNHDPHSAIIDASQTRVSGQRLVNILAWFDNEWSFANRMLDVAEYYLCIASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL50</accession>
    <name>A0A481QL50_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Iron-sulfur cluster insertion protein ErpA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">erpA</name>
        <name evidence="7" type="ORF">C3B55_00130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for insertion of 4Fe-4S clusters for at least IspG.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
        <text evidence="5">Binds 1 iron-sulfur cluster per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00130"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01380">
        <property type="entry name" value="Fe_S_insert_ErpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="FeS_cluster_insertion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023063">
        <property type="entry name" value="FeS_cluster_insertion_RrpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00049">
        <property type="entry name" value="TIGR00049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S_biosyn" evidence="6">
        <location>
            <begin position="9"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="116" mass="12521" checksum="574D33E502D1A396" modified="2019-06-05" version="1">MSVKFYAPMTLQVTHSAVHKVKSLVNEAGNDRLKLRIFVKGGGCSGFQYGFTVDEDIADDDTIVECEGVSLVVDSMSFQYLAGSEVDYQESLAGSRFMIKNPNATTTCGCGSSFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL51</accession>
    <name>A0A481QL51_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative inner membrane transporter YedA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yedA</name>
        <name evidence="3" type="ORF">C3B55_00346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00346"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="15"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="152"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="298" mass="32009" checksum="6B496BB12D7CA2EE" modified="2019-06-05" version="1">MLGLHRFPASLIAAFFSLYVIWGSTYLAIRIGVEYWPPLMLAGIRFLVAGLAMYSFLRWRGAPTPTWLQWKAAAKIGILLLTFGNGAVTVAEYIGVASGVAALAVATVPLFTLLFGYFWGARNTRLEWAGVVLGIIGIVMLNIGSNLQSSQTGALLLVFAAAAWAFGSVWSKHLPLPAGAMASALEMIIASALLLAGSALTGERLYAMPPIEGWFALAYLIVFGSIIAFNAYMYLLKHVRLAAATSYAYINPAAAVLLGIIFVGETIGMEEVLAMLVIISAVLLISLPQWRKPKSDIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL53</accession>
    <name>A0A481QL53_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="6">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">tsaD</name>
        <name evidence="8" type="ORF">C3B55_00113</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00113"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="PTHR11735:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03723">
        <property type="entry name" value="T6A_TsaD_YgjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TsaD" evidence="7">
        <location>
            <begin position="24"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="134"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="341" mass="36666" checksum="3C966BF7EC0D73DE" modified="2019-06-05" version="1">MLVLGIETSCDETGIALYDNERGLLSDVLFSQISLHRAYGGVVPELASRDHVKRILPLIRKVFDKACCVPTDLDAIAYTAGPGLVGALLVGASCAQALAFAWGIPVLGVHHMEGHLLAPMLEKTPPQFPFVALLVSGGHTQLVKVDGIGQYILLGESIDDAVGEAFDKTAKMMGLNYPGGPEISRLAEKGVLGRYTFSRPMCDRPGLMFSFSGLKTTALQAWKHSVSAGDNSEQARCDISMAFQLAVVETLAIKCKRAMKQTGMQRLVVAGGVSANKALRTSLKEMLGDINCDVFYARPEFCTDNGAMIAYAGCQRLQVGQHNGLTISVQARWPIEQLVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL55</accession>
    <name>A0A481QL55_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA sulfurtransferase</fullName>
            <ecNumber evidence="11">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 15" type="primary">thiI</name>
        <name evidence="15" type="ORF">C3B55_00169</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ThiI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00169"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA U4 sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026340">
        <property type="entry name" value="Thiazole_biosynth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04271">
        <property type="entry name" value="ThiI_C_thiazole"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00342">
        <property type="entry name" value="TIGR00342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8 11" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="4 11 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="14">
        <location>
            <begin position="63"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="13">
        <location>
            <begin position="405"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="11">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="185"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX81535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="485" mass="55118" checksum="B2F48759BA16927C" modified="2019-06-05" version="1">MKLIVKVFPEIIIKSRLVRMRFIRKLAKNIRAVLHDLDQAVVVSGMWDYLELETHITDAKILKSMIERLSSMPGIAYFLRVDEYPLGSLDDITEKCKQHYGESLAGKMFSVRCKRAGKHPFSSMDVEQYVGSKLHRECSAAGISLKMSQIEVRIEIRDQRLFVVHSQHNSIGGYPLGVLEQTLVLISGGFDSTVAAYQIIRRGLMSHFCFFNLGGRAHELGVMEVVHFIWKKYSSSQRVLFVSVPFEEVLGEILNKVDNSYMGVVLKRMMLRAASQIADQLHIDALVTGEAISQVSSQTLPNLSLIDCVTEKLVLRPLIASHKQDIINLAEQIGTADFAKYMPEYCGVISVSPKTHAKRYRVEHEEKKFDMAILERALKNARLVPIDRVIDELGQDLQIEEVTEILAGQVVVDIRHPDAAEYEPLKITGIDVKMLPFYALNKCFKELDNSRQYLLYCDKGVMSRLHAHHLLSEGYVNVRVYRPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL84</accession>
    <name>A0A481QL84_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">rplR</name>
        <name evidence="8" type="ORF">C3B55_00293</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00293"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_B">
        <property type="entry name" value="Ribosomal_L18_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004389">
        <property type="entry name" value="Ribosomal_L18_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="PTHR12899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00060">
        <property type="entry name" value="L18_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="116" mass="12622" checksum="52602817EC7E8758" modified="2019-06-05" version="1">MTGKKVSRLRRARKARLKMHELEIVHLCVFRSSQHIYAQVVSASGNEVMASASTLDKKLRDGATGNINAATKVGHIIAMRAKAAGVSRVAFDRSGFKYHGRVKALADAAREAGLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL89</accession>
    <name>A0A481QL89_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acyltransferase YihG</fullName>
            <ecNumber evidence="3">2.3.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yihG</name>
        <name evidence="3" type="ORF">C3B55_00416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00416"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="PlsC" evidence="2">
        <location>
            <begin position="86"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="299" mass="35021" checksum="45C504008E2172C3" modified="2019-06-05" version="1">MLDFLPTTVRGIIALLLLALNTILLCSFLFIIALFKALPFAKHFSEWLINCTHEAWITNNKGWMNLVQRTRWHLSGLEGLNYQHSYLVTSNHQSSVDIMVLQYVLNRRIRPLKFFLKQELIWVPIIGLAWWALDFPFMKRYSKAYLEKHPEKQGKDLKTTHKNCVKFRDKPVGIFNFSEGTRFTFSKHTQQKSPFRYLLKPKAGGIAFVLDAMGGQLKSLVNVTIHYPAGCPGYWDLLCGKVKDVVVHFEEVQILTEFIGKNYTRDREYRLALHSWINELWKQKDALLERQHKDFFGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL92</accession>
    <name>A0A481QL92_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00246"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="49" mass="5660" checksum="F1088DAB822F9692" modified="2019-06-05" version="1">MVRLSKLLLGKPYSMFVIDHFKAIIRQLSGSSYACWLMVLIFQSLTKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL94</accession>
    <name>A0A481QL94_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Riboflavin biosynthesis protein RibD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Diaminohydroxyphosphoribosylaminopyrimidine deaminase</fullName>
                <shortName evidence="10">DRAP deaminase</shortName>
                <ecNumber evidence="10">3.5.4.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">Riboflavin-specific deaminase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">5-amino-6-(5-phosphoribosylamino)uracil reductase</fullName>
                <ecNumber evidence="10">1.1.1.193</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">HTP reductase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="primary">ribD</name>
        <name evidence="15" type="ORF">C3B55_00311</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H(+) + H2O = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17845"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58421"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="EC" id="1.1.1.193"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 13">Binds 1 zinc ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">In the C-terminal section; belongs to the HTP reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.26" evidence="10"/>
    <dbReference type="EC" id="1.1.1.193" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00311"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00401"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008835">
        <property type="term" value="F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004794">
        <property type="entry name" value="Eubact_RibD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006769">
        <property type="entry name" value="RibD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00326">
        <property type="entry name" value="eubact_ribD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="8 10 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="14">
        <location>
            <begin position="10"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="309"/>
            <end position="315"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX81666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="379" mass="40829" checksum="BC9525703FE62EE8" modified="2019-06-05" version="1">MNLPTTEQSVIDSHYMARALELARKGLYTTHPNPRVGCIIVHDGHIIGEGWHERTGEPHAEMHALRTTGDKACGSTVYVTLEPCSYHGHTPPCVHALVTAGVARVVAGTLDPNPKVAGRGLQRLAKAGIDIHSGVLEKEARALNPGFFKRMKYGLPFVRVKLAMSLDGRTAMANGKSQWITGPATRSAVQRLRAEASVVLTGADTVLVDGARLVVCATELGLDEATTALAMSRLPIRVLIDGRLRVPLNAPFFKAGPALVVTCVPSENQFLRGDHEYLVVPCLNGYVDLHSALVALAARDVNEVLVEAGASLAGAFAQQDLVDEYVIFIAGKFLGSTARPLLDWPLEKLADAPRLKITRVRAIGDDWRITAMPVRIVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLA2</accession>
    <name>A0A481QLA2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin prenyltransferase UbiX</fullName>
            <ecNumber evidence="5">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ubiX</name>
        <name evidence="7" type="ORF">C3B55_00321</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl phosphate + FMNH2 = phosphate + prenyl-FMNH2</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00321"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="PTHR43374"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 5" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavoprotein" evidence="6">
        <location>
            <begin position="7"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="209" mass="22617" checksum="FD161C1CCD01495C" modified="2019-06-05" version="1">MSSPESVTLAMTGASGAPYGLRLLDCLVREDREVRFLISNAAQLVMAIETDVQLPSKVHMMQAFLTEYTGAAVGQIKIYGTEDWMSPVSSGSSTPSPMVVVPCSTGALSAIATGACNNLIERAADVTLKERRQLILVPREAPYSSIHLEHMLKLSNIGVTILSASPGFYHQPQTIDDLVDFVVARVLNLLNIPQDMLSCWGECHLGNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLA7</accession>
    <name>A0A481QLA7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5 7">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">rpsL</name>
        <name evidence="9" type="ORF">C3B55_00272</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5 7">Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1 5 7">With S4 and S5 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 7">Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00272"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_B">
        <property type="entry name" value="Ribosomal_S12_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_S12_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="122" mass="13680" checksum="DE8EB0D4EEBDD3D5" modified="2019-06-05" version="1">MTTINQLVRQPRKRIVEKSDVPALQNCPQRRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTIRGSLDTSGVKGRHQGRSKYGTKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLG3</accession>
    <name>A0A481QLG3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">rplB</name>
        <name evidence="7" type="ORF">C3B55_00280</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00280"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_B">
        <property type="entry name" value="Ribosomal_L2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_L2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L2_C" evidence="6">
        <location>
            <begin position="125"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="225"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="274" mass="29654" checksum="59A06CD2C33830FD" modified="2019-06-05" version="1">MAIVKCKPTSPGRRFMVKVVNHELHKGAPYAPLLEKKSKTGGRNNNGRITTRHIGGGHKQHYRLVDFCRNDKDGITATVESIEYDPNRTAHIALLLYADGERRYIIAPKGVSAGDQLIAGALAPIKPGNAQQLRNIPVGSTIHGIELKPGKGAQIARSAGASAQLIAREGTYVTIRLRSGEVRKVISECRATLGEVSNSEHSQRSLGKAGAKRWCGVRPTVRGVAMNPVDHPHGGGEGRTSGGRHPVSPWGFPTKGAKTRSNKRTDKMIVRHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLG5</accession>
    <name>A0A481QLG5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="7">GSA</shortName>
            <ecNumber evidence="7">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="7">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">hemL</name>
        <name evidence="9" type="ORF">C3B55_00342</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00342"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="432" mass="46544" checksum="85AC7F3E8CC59932" modified="2019-06-05" version="1">MSRSTTLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAEGAYITDEDDKRYVDYVGSWGQMILGHSHPNVLDAVRQQLQHGLSYGAPTAIETEMADILCSIVPSMEMVRMVSSGTEATMSAIRLARGFTGRDNIIKFEGCYHGHSDSLLVKTGSSAMTIGMPNSAGVPRAFAKHTLNLPFNDITFVKQILNEVNQNIACIIVEPVAGNMNCVPPIPGFLEGLREQCDKYGIVLIFDEVMTGFRVALGGAQAYYSVMPDLSTFGKIIGGGMPVGCFGGKREIMQQIAPLGPVYQAGTLSGNPLAMAAGLTTICLLNQPGFYGELTNYTMNLLDGLQQRADTAGIPFITTQAGGMFGLYFSDADKISNFNDVMDSNLNLFKRFFHLMLEDGVYLAPSAFEASFTSIAHGSTELKLTLNAAERAFKALNKQAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLG9</accession>
    <name>A0A481QLG9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00064"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="43" mass="4880" checksum="E6E90D29FB794999" modified="2019-06-05" version="1">MVTLRANKDMYLTHKVIVVVKSSNIAFEYVIAKALLKLLGFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLI4</accession>
    <name>A0A481QLI4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 8">Transcription elongation factor GreA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcript cleavage factor GreA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">greA</name>
        <name evidence="11" type="ORF">C3B55_00363</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7 8">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the GreA/GreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00363"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006359">
        <property type="entry name" value="Tscrpt_elong_fac_GreA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01462">
        <property type="entry name" value="greA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00829">
        <property type="entry name" value="GREAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00830">
        <property type="entry name" value="GREAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GreA_GreB_N" evidence="10">
        <location>
            <begin position="5"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="GreA_GreB" evidence="9">
        <location>
            <begin position="83"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="158" mass="17374" checksum="5A08666BC8F60247" modified="2019-06-05" version="1">MIKYPMTVEGFNALKKEHAYLIKVVRPKLSQDIGAARALGDLKENAEYHAAREQQGMVEARIRNIEGRLHNSVVIDIKTIPKTGKVLFGTTVKIINVKTDEIVVYHIVGEDEADFKLGKISSSSPLASALIARAEGDVVVVKTPGGLIEYEIIEVNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLI5</accession>
    <name>A0A481QLI5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6 7">50S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">rplJ</name>
        <name evidence="8" type="ORF">C3B55_00268</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and the large rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00268"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00362">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022973">
        <property type="entry name" value="Ribosomal_L10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002363">
        <property type="entry name" value="Ribosomal_L10_eubac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="PTHR11560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01109">
        <property type="entry name" value="RIBOSOMAL_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="168" mass="18174" checksum="C47568546E5C5965" modified="2019-06-05" version="1">MAINLEDKKVIVAEVNEAAKAALSAVVVNARGVTVGAMIKLRKEAREAGVYIRVVRNTLLKRAVAETEYSILNDVFTGPTMIAFSKEYPGAAARLFKEFAKSQNKLEIKAAAFEGKFLTVDQVDILATFTTRNEAISQLMSVIQGATSKLAYTLALVFKQKESAASLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLI8</accession>
    <name>A0A481QLI8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 7">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">rpsI</name>
        <name evidence="8" type="ORF">C3B55_00538</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00538"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_B">
        <property type="entry name" value="Ribosomal_S9_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_S9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="130" mass="14667" checksum="DACF361C5A0F4B15" modified="2019-06-05" version="1">MPTIQNYGTGRRKTATARVFLRPGTGNILINNRSLDNFFGRETAHMIVRQPLELTDTLEKFDIYVTVIGGGVSGQAGAIRHGITRALMQYDKTLRGALRKASFVTRDAREVERKKVGLRKSRKCPQYSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLJ5</accession>
    <name>A0A481QLJ5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00320"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="40" mass="4610" checksum="F918B431BA87700C" modified="2019-06-05" version="1">MCMRILGIYSTFMGLMISLLKDLGYHVINYNNDILLLTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLK4</accession>
    <name>A0A481QLK4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">rplX</name>
        <name evidence="6" type="ORF">C3B55_00288</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="3">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00288"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_L24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="PTHR12903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="110" mass="12263" checksum="59BF7E433BA65757" modified="2019-06-05" version="1">MHKIRRDDEIIVIAGKEKGKRGKVLKVFTDNRLVVSGLNLIKRHTKPNQMLGAQGGIVKTEAPLHVSNVAVFNSETNKADRIGFKVKDGKKIRVFKSTQQEVDVLTMLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLL0</accession>
    <name>A0A481QLL0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5 7">Bacterioferritin</fullName>
            <ecNumber evidence="5">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">bfr_1</name>
        <name evidence="9" type="ORF">C3B55_00305</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>4 Fe(2+) + 4 H(+) + O2 = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 7">Belongs to the bacterioferritin family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00305"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:cellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00907">
        <property type="entry name" value="Bacterioferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002024">
        <property type="entry name" value="Bacterioferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002560">
        <property type="entry name" value="Bacterioferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00601">
        <property type="entry name" value="BACFERRITIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00754">
        <property type="entry name" value="bfr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00549">
        <property type="entry name" value="BACTERIOFERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0409">Iron storage</keyword>
    <keyword evidence="5 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="8">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR002560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002560-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="154" mass="17962" checksum="84C99DB8DCA68B33" modified="2019-06-05" version="1">MQGHPDVIDYLNTLLISELVARNQYFIHSRMYEGWGFNELYKRINHEMNEESLHVDALICRILVLEGTLRVRSEDVYVGITVPDMLAADLRLEYKACAALRKGIELCEQHNDYVTSAMLQVQLNDTEIDHTYWLEKQLSLIKLIGLENYMQSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLN8</accession>
    <name>A0A481QLN8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00569"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="45" mass="5313" checksum="1ADA1DAD38186238" modified="2019-06-05" version="1">MPSMLLALNDIITVISPYRLKSSRYLRHSHKNIIVMVFWLSKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLP0</accession>
    <name>A0A481QLP0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00568"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="60" mass="7038" checksum="D177CAC6B57C92DD" modified="2019-06-05" version="1">MRFQCRYYILRCLYMFSMFRYDDLEFLRFSSKCDLVLLLTFVATAEGSTGVLETGLSDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLP9</accession>
    <name>A0A481QLP9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Protein GrpE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">HSP-70 cofactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">grpE</name>
        <name evidence="6" type="ORF">C3B55_00356</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 5">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00356"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_coiled_coil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="PTHR21237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="SSF51064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="SSF58014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 6" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="184" mass="20827" checksum="AD3A0BF88F22763E" modified="2019-06-05" version="1">MAEEQTQDTQILDANLVTDDALVTRVQLLEEQLAAAQDQSLRIAADLQNVRRRAEQDVEKAHKFSLEKFAHDLLPIIDSLERGLELSNPDDENIRPMREGIELTLKMFQDTMKRYQLEAIDLQGGEPFNAEHHQAMAMQESNDLEPNSILKVFQKGYQLNGRLLRPAMVVVSKAPAHVAPSIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLQ2</accession>
    <name>A0A481QLQ2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00185"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="52" mass="5700" checksum="E3FEAA670D7E0771" modified="2019-06-05" version="1">MLVTLALIRLVNVLKGCAGYNSAAICLNVLDNAYGAYRLSAFQFITIFWLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLQ9</accession>
    <name>A0A481QLQ9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="8">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aceF</name>
        <name evidence="11" type="ORF">C3B55_00195</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + acetyl-CoA = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:17017"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="2.3.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms a 24-polypeptide structural core with octahedral symmetry.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00195"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006256">
        <property type="entry name" value="AcTrfase_Pyrv_DH_cplx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178:SF2">
        <property type="entry name" value="PTHR43178:SF2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 8" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="96"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="10">
        <location>
            <begin position="191"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="492" mass="52773" checksum="3858A31E9C5A3A2D" modified="2019-06-05" version="1">MSELIRVPNFGNNKGRVIELLVKEGDTIQIDQSILILESNKTNIEIPALKAGLIKNLKVKPGDWLQEGDELLELETVDVVAAPASTAKKPITITNFQDILVPDIGSSNKAKIIELLVTTGDIVEVDQSLITLECDKASMEIPSPATGVVESIAVKLTDEVSAGDFILKLKTQDAVMAPAAKAATLSVTKVHAGPAVRKLARELGVELHSVSPTGPHGRVLKEDVQVYVKAMTQKATKNISANAANGGINIPSIPIAHLSCFGETEETPMTRLMQIGGPSLHRNWLNIPHVTQFDQADITDLKAFCVAQKAAAQQAGVRLTLLPMLIKACAHLLKELPDFNSSLASSGKAIIRKKYVHIGFAVDTQDGLLVPVIRSVDQKSLLKLAAEASALTTKARDKKLTADDMRGACFTVSNLGHISGTGFTPIVNAPEVAILGISKAIIQPIWDGTAFQPKLMLPLSLSYDHRVINGAAAAHFTRRLSELLNDIRTLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLR1</accession>
    <name>A0A481QLR1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00434"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="116" mass="13171" checksum="F512DD3C44FA0A5A" modified="2019-06-05" version="1">MTEQTISCLSRSGNKVKGCLHSNPFLIELNTTSVFIMHIEYRKNLTLITTVNIILPIETCDASHPTNINNNFYACKAIGFQQTTYPTNIPNYHIRILIALIKQIVKLTTAKARFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLS7</accession>
    <name>A0A481QLS7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA synthetase</fullName>
            <shortName evidence="8">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">serS</name>
        <name evidence="10" type="ORF">C3B55_00457</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00457"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43697">
        <property type="entry name" value="PTHR43697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="171"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Serine binding" evidence="8">
        <location>
            <begin position="233"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="264"/>
            <end position="266"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="351"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="426" mass="47532" checksum="E6B27D3A0BF0B24A" modified="2019-06-05" version="1">MLDLKWLRNNLYNAADRLASRGFALGVERFKVLEEQRKSVQVHTEALQAQRNACSKYIGQAKQRGEDIASLMVDVERIGSELSNSKVKLEKIHSELHSILLGIPNIPHESAPIGKDKDGNVEVRRWGIPKSFDFEIRDHVAIGKLTGDLDCETAAKMSGARFALLRGPIARMHRALAQFMINLHTNEHGYEEAYTPYLVNASALLGTSQLPKFKDDLFKISRDGASDLYLIPTAEVSLTNIVAHEILDIRLLPIKFVTHSPCFRSEAGASGRDTHGMMRQHQFDKVEIVQVVEPSTSMQALEDLTVNAERVLQLLNLPYRVLALCTGDMGFSALKTYDLEVWIPSQDKYREVSSCSNCGDFQARRMHARFRNPESGKLELVYTINGSGLAVGRTLVAVLENYQQADGSIRVPEVLKPYMAGVEVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLT4</accession>
    <name>A0A481QLT4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tsaE</name>
        <name evidence="5" type="ORF">C3B55_00226</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TsaE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00226"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003442">
        <property type="entry name" value="T6A_TsaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02367">
        <property type="entry name" value="TsaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00150">
        <property type="entry name" value="T6A_YjeE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="155" mass="17624" checksum="AF46367A27C390D7" modified="2019-06-05" version="1">MFDVTLLLADENAMVLFGHRIAQVTTGVGLVFIEGDLGVGKTTLSRGIIRGLGYIGVVKSPTFTLVEPYEIGVIRAYHFDLYRLIDHEELEYMGIRDYLEKDALCLIEWPDKNICFLPKPDLTITIIPHKYGRHLTLLPHSMRGQFWCIALALEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLT9</accession>
    <name>A0A481QLT9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">OMP decarboxylase</fullName>
            <shortName evidence="7">OMPDCase</shortName>
            <shortName evidence="7">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">pyrF</name>
        <name evidence="10" type="ORF">C3B55_00647</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7 8">
            <text>H(+) + orotidine 5'-phosphate = CO2 + UMP</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7 8">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the OMP decarboxylase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00647"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01200_B">
        <property type="entry name" value="OMPdecase_type1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="PTHR32119:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7 8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="233" mass="25163" checksum="7DC72D06E0B8394B" modified="2019-06-05" version="1">MSICQTPIIVAMDYPTSAAALNLANQLDPKLCRVKVGKELCINGATEIVKTLSDKYFEVFLDLKFHDIPNTTALAVKAATEMGVWMVSVHCSGGLRMMSACREVLEQYTGQKPLLIGVTVLTSMKREDLAEIGLDIEPQEQVLRLAALARRAGFDGLVCSALEVPALKAAYPELQVVTPGIRQAGSTQDDHRRIFTPRQALDAGSDYLVIGRPISQVVDPAQALAEIVASIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLU2</accession>
    <name>A0A481QLU2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">30S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">rpsF</name>
        <name evidence="10" type="ORF">C3B55_00237</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Binds together with S18 to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00237"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00473">
        <property type="entry name" value="bS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00360">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020815">
        <property type="entry name" value="Ribosomal_S6_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020814">
        <property type="entry name" value="Ribosomal_S6_plastid/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_S6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="PTHR21011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="SSF54995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01048">
        <property type="entry name" value="RIBOSOMAL_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="100"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="131" mass="15427" checksum="BDF0AA6F04EA2D08" modified="2019-06-05" version="1">MRYYEIIFLVHPDQSEQVGGMVERYTKLIEEDGGKIHHLEDWGRRQLAYAINNVHKAHYVMLNVECTSKSLAELKDNFRYNDAVIRNLIIRREEVFTGQSEMLKVEENRNDRRDRSGHESTDSDHSDSSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLW0</accession>
    <name>A0A481QLW0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protease HtpX</fullName>
            <ecNumber evidence="12">3.4.24.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Heat shock protein HtpX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">htpX</name>
        <name evidence="14" type="ORF">C3B55_00678</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00678"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 12 14" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0346">Stress response</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="13">
        <location>
            <begin position="82"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX82002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="296" mass="32703" checksum="E3DF4F37BE3EF68C" modified="2019-06-05" version="1">MMRILLFLATNLAVVLIANITLSLFGFNGFMTTNGIDLNLDQLLIFCAVFGFAGSLISLFISKWMAKMSTNIQVINQPRTRHEQWLLQTAEQLSREAGIKMPEVGIFPAYEANAFATGWNKNDALVAVSQGMLERFSYDEVKAVLAHEIGHVANGDMVTLALVQGVVNTFVMFFARIVGNFVDKALFKNEEGHGIAYFAATVFAEVVLGFLASAITMWFSRRREFRADEVGACLAGTRAMVAALQHLRSEQGLPVSMPNSLIAFGINSGIKQGFTRIFMSHPPLEERIDALRQLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLW3</accession>
    <name>A0A481QLW3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">30S ribosomal protein S20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">rpsT</name>
        <name evidence="10" type="ORF">C3B55_00417</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Binds directly to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00417"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_S20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_S20_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="PTHR33398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="SSF46992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="95" mass="10649" checksum="B8AAB0A8001085B4" modified="2019-06-05" version="1">MANTPSAKKRTKQIEKRRNHNASLRSMVRTYIKSVAKAIDTKNAKTAQAAYIIAVPIIDRMADKGIIHKNKAARHKSRLNSHIKALSVFAVTQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLW4</accession>
    <name>A0A481QLW4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="5">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glyoxalase II</fullName>
            <shortName evidence="5">Glx II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">gloB</name>
        <name evidence="7" type="ORF">C3B55_00489</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00489"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00676"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005457">
        <property type="entry name" value="Glx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="6">
        <location>
            <begin position="12"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="255" mass="28666" checksum="FB7ACB71FAB614ED" modified="2019-06-05" version="1">MIQISALPAFTDNYLWLLKNLKTKHCAVVDPGVASPVLAWLAQNPAWMLRDILITHYHYDHVGGVEQLKIITGAKVYCPANEKIPTWDVAIQNNDRISILGWDFDIYSLPGHTLGHIAYYHQGVLFCGDTLFAAGCGRLLEGTPEQMYSSLQRLATLPANTLVYCAHEYTQSNLKFAQAVEPDNADIAERVTNVKKLLARGKVTLPSNLALEKRTNPFLRTAEISVKQKADEWSSFNNRFGVEVFTSLRAWKDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLW5</accession>
    <name>A0A481QLW5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferases group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00688</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00688"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015393">
        <property type="entry name" value="DUF1972"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09314">
        <property type="entry name" value="DUF1972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF1972" evidence="2">
        <location>
            <begin position="12"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="199"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="374" mass="42370" checksum="02E185B99A572CE9" modified="2019-06-05" version="1">MCLQNSMIFKLAIIGTVGLPAKYGGWETLSEQLIIYLDQKYEISLYCSSMSYASQLVKYRNANLIYLPLQANGVQSIFYDIVSMLHAYRKNDVLLILGVSGCVALPFLKLLTNNKIVVNIDGYEWKRTKWSALAKKFLKFSEAVAVKYSDAIITDNKVIQDYVRSEYRRESTLITYGGDHAITSILSKSDLQRYPLLAQPYAFSVCRIEPENNIHVIIEAFNTSSLPLIIVGNWKYSEYGKVLQDKYANKQHLHLLDSIYNSDELNLLRSNATIYIHGHSVGGTNPSLVEAMWLSLPIIAFDVSFNRISTEGCAKFFSSSQALIDSAVNLFANQAERAQLSKKMHEIAVRRYRWDSISAQYDNLFKSLLLSNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLX3</accession>
    <name>A0A481QLX3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00501</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00501"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="70" mass="8408" checksum="D35EC604DC3ACF52" modified="2019-06-05" version="1">MNNDNATFSHKINKKYSGIDYYNDTRQQYAFCLLFMQIDTALYLHRLVVNMQHSLTLFNFKVAWFIHPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLX9</accession>
    <name>A0A481QLX9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">50S ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">rplQ</name>
        <name evidence="6" type="ORF">C3B55_00304</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Contacts protein L32.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00304"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_L17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="PTHR14413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="SSF64263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="120" mass="13814" checksum="13234082C62CD262" modified="2019-06-05" version="1">MRHRKSGLHLSRTSSHRKAMFQNMAVSLFEHELIKTTLPKAKELRRIAEPLITLAKIDSLANRRLAFDRTRSKAIVGKLFNYLGKRYISRHGGYLRILKCGFRSGDNAPMAYVELVDRAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLY4</accession>
    <name>A0A481QLY4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein-releasing system ATP-binding protein LolD</fullName>
            <ecNumber evidence="6">7.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">lolD</name>
        <name evidence="8" type="ORF">C3B55_00709</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the ABC transporter complex LolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone, LolA. Responsible for the formation of the LolA-lipoprotein complex in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The complex is composed of two ATP-binding proteins (LolD) and two transmembrane proteins (LolC and LolE).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC transporter superfamily. Lipoprotein translocase (TC 3.A.1.125) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.-" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00709"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044873">
        <property type="term" value="P:lipoprotein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015854">
        <property type="entry name" value="ABC_transpr_LolD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011924">
        <property type="entry name" value="LolD_lipo_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220:SF654">
        <property type="entry name" value="PTHR24220:SF654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02211">
        <property type="entry name" value="LolD_lipo_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="7"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="227" mass="24804" checksum="6C1D37BEE0A2FB0F" modified="2019-06-05" version="1">MSEKAILSCFNLGKSYDEGLESVVVLSNLHLELYPGERLAIIGSSGSGKSTLLKLLGGLDTPSQGNIWLAGEELSTLGEKSRSQLRNRSLGFVYQFHHLLPEFTALENVCMPLLIGKTVISEARHHAKTLLERVGLGHRLEHKPAELSGGECQRVAIARALINNPGLVMLDEPTGNLDSCTAQSIKDLILELSSEMCTTFLVVTHDMSLARQMDRLLHLQEGHLVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLY6</accession>
    <name>A0A481QLY6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="2">TMP kinase</shortName>
            <shortName evidence="2">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="2">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 5" type="primary">thiL</name>
        <name evidence="5" type="ORF">C3B55_00314</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + thiamine phosphate = ADP + thiamine diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00314"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02128">
        <property type="entry name" value="TMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01379">
        <property type="entry name" value="thiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AIRS" evidence="3">
        <location>
            <begin position="29"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="4">
        <location>
            <begin position="150"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="121"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="322" mass="33403" checksum="09FDEB16AD9C1660" modified="2019-06-05" version="1">MSEFDLIRKYFTAAPCAQRGEGITLGIGDDCALLALPAGEQLAISTETLVAGVHFADPCDSFLLGQRSLAVAVSDLAAMGAYPLAFTLALTIPTANIDWLQGYVHGLNVMAKSCNVGLVGGDTTRGSLSLTITVFGRVPTGQELTRSGARPGDLLCVGGTLGDASGALPLVLGQRTADIAIAQPLLAHYWSPQPQLALGLALRGKATSAIDISDGLLADCEHIAKASAVSLVVERQKLPLSYALIAFAGDDKAYMAALNGGDDYVLVFTVPPVELVALLASGWPVYVIGRVEVGHGGVTLLDAIGQDITPAVRGYQHFCETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLZ3</accession>
    <name>A0A481QLZ3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Erythronate-4-phosphate dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.290</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 10" type="primary">pdxB</name>
        <name evidence="10" type="ORF">C3B55_00719</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>4-phospho-D-erythronate + NAD(+) = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:18829"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="ChEBI" id="CHEBI:58766"/>
            <dbReference type="EC" id="1.1.1.290"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.290" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00719"/>
    <dbReference type="UniPathway" id="UPA00244">
        <property type="reaction ID" value="UER00310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033711">
        <property type="term" value="F:4-phosphoerythronate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12158">
        <property type="entry name" value="ErythrP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01825">
        <property type="entry name" value="PdxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020921">
        <property type="entry name" value="Erythronate-4-P_DHase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024531">
        <property type="entry name" value="Erythronate-4-P_DHase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038251">
        <property type="entry name" value="PdxB_dimer_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11890">
        <property type="entry name" value="DUF3410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="7">
        <location>
            <begin position="25"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="8">
        <location>
            <begin position="107"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3410" evidence="9">
        <location>
            <begin position="288"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="205"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="380" mass="41581" checksum="5E62EBDB310561BC" modified="2019-06-05" version="1">MLIVADENISLLDVFFQGFGEIHRVSGRSINHATVKSADVLLVRSVTNVDRTLLTGTKVRFVGTCTIGTDHLDLDYFQQAGIAWSSAPGCNARGVVDYVLSSLQTLAEITGANLSQCTYGIVGVGKVGGLLLKVFKGLGWNVLACDPLLQIIAGGDYVNLTQIIEQCDVISLHTPLTKSGNSSTWHLFDRQRLNQLRLGAWLINASRGQVVDNAALREVLLKRDDLQVVLDVWDGEPEVDVDLADLCALATPHIAGSSLDGKQRSTAKIYQALCVYLGQEPSIQLSDFLPQPWLAEVHLDASTDLAWALAILCRSVYDPRRDDADFRRSLVGTAETQRKAFDLLRNNYPERREIDSLKVHINGQSTQLMKIVSALGAITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM04</accession>
    <name>A0A481QM04_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe/S biogenesis protein NfuA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">nfuA</name>
        <name evidence="8" type="ORF">C3B55_00474</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in iron-sulfur cluster biogenesis. Binds a 4Fe-4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster per subunit. The cluster is presumably bound at the interface of two monomers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NfuA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00474"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01637">
        <property type="entry name" value="Fe_S_biogen_NfuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017726">
        <property type="entry name" value="Fe/S_biogenesis_protein_NfuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03341">
        <property type="entry name" value="YhgI_GntY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S_biosyn" evidence="7">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="NifU" evidence="6">
        <location>
            <begin position="114"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="194" mass="21416" checksum="F44D51B03FD47464" modified="2019-06-05" version="1">MTAITITDAAHDYLADLLSKQNTPGIGIRIFITQPGTQYAETCITYCKPGEEKSKDIALKLKSFTVYIDYFSKVFLKEAVVNYTIDRIGGQLTIKAPNAKVPSITVDSPINDRINYYLQTEINPGLANHGGQISLIDVVEDSIAVLRFGGGCQGCGQADKTLREGIERTLIERIPELKGVRDITDHAQKENAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM05</accession>
    <name>A0A481QM05_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00494"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="65" mass="7469" checksum="F9D27874A612E556" modified="2019-06-05" version="1">MFSSDAHNDTLAKINFNDRVTFFGINCDSAIIRASLCLLVLVLVVFYFRLGLLYRFSLDYIILLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM19</accession>
    <name>A0A481QM19_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enolase</fullName>
            <ecNumber evidence="8">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 13" type="primary">eno</name>
        <name evidence="13" type="ORF">C3B55_00752</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell surface</location>
        </subcellularLocation>
        <text evidence="8">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00752"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
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        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
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    </dbReference>
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        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 8 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Enolase_N" evidence="12">
        <location>
            <begin position="58"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase_C" evidence="11">
        <location>
            <begin position="195"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="420"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 9">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="393"/>
        </location>
    </feature>
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        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="8">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
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            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX82071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="481" mass="52346" checksum="7A6DE8B9509B057E" modified="2019-06-05" version="1">MFLLLRILRKMLSSISLVLVVMPTRWSCYRCYTIDGLKISLKYVIFVNFRVFTVMAKIIDIKGREVLDSRGNPTVEADVLLDNGIIRSACVPSGTSTGSREALELRDGDKSRYLGKGVLKAVSNINGPICDLLLGNDPLDQKSIDYAMIKLDGTENKSCLGANAILAVSLAVAKAASHGMPLYAYIANLNGTPGFYSMPVPMMNIINGGKHADNNVDIQEFMVQPIGAKTFSDGLRIGTEIFHNLKVVLQSYGLSTAVGDEGGFVPSLASNEDALKMISEAVVNAGYKLGTDVTMSIDCAASEFYENGTYHLFSEGYTFTSDGFSDYLKDLTERYPIISIEDGLDESDWVGWKVLTDKIGEKVQLVGDDLFVTNTKILKEGIDKKIANSILIKLNQVGTLTETLEAIQMAKVAGYTVVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVSKYNQLLRIEEQLAGNVQYNGRSEFHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM23</accession>
    <name>A0A481QM23_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lipopolysaccharide export system ATP-binding protein LptB</fullName>
            <ecNumber evidence="5">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">lptB</name>
        <name evidence="5" type="ORF">C3B55_00524</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.-" evidence="5"/>
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        <property type="protein sequence ID" value="QAX81858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pade:C3B55_00524"/>
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        <property type="component" value="Chromosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03218">
        <property type="entry name" value="ABC_YhbG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030921">
        <property type="entry name" value="LPS_export_LptB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04406">
        <property type="entry name" value="LPS_export_lptB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="241" mass="26614" checksum="D393446FD784B457" modified="2019-06-05" version="1">MATLKSQHLAKSYKSRDVLRDVSLSISSGQIVGLLGPNGAGKTTCFYIIVGLVKADYGRILIDDLDVSDQPMHGRARAGIGYLPQEASIFRKLSVSDNIMAILETRKELNRDGRRKELESLLQEFHINYIRSNLGMSLSGGERRRVEIARSLANAPKFILLDEPFAGVDPISVYDIKQIIHHLAARGIGVLITDHNVRETLDICETAYIINDGKLIAEGNSATILSNELVKAVYLGNEFHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM35</accession>
    <name>A0A481QM35_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Intermembrane phospholipid transport system permease protein MlaE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mlaE</name>
        <name evidence="11" type="ORF">C3B55_00519</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex MlaFEDB, which is involved in a phospholipid transport pathway that maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The complex is composed of two ATP-binding proteins (MlaF), two transmembrane proteins (MlaE), two cytoplasmic solute-binding proteins (MlaB) and six periplasmic solute-binding proteins (MlaD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the MlaE permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00519"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003453">
        <property type="entry name" value="ABC_MlaE_Proteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030802">
        <property type="entry name" value="Permease_MalE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30188">
        <property type="entry name" value="PTHR30188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02405">
        <property type="entry name" value="MlaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00056">
        <property type="entry name" value="TIGR00056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="265" mass="28679" checksum="03FFC4D9C1B2B680" modified="2019-06-05" version="1">MRKTSILEQVRLFGRSGLDLVKVLGRSIIFLFYTLFGRNNTCSNFSMLIKQLHSVGVMSMVIIIVSGIFIGMVLALQGFNILSSYGSERAVGQMVALTLLRELGPVVTALLFAGRAGSALTAEIGNMKSTEQLSSLEMLGVDPLKYIIAPRLLAGLISLPLLTMIFSVMGIWGGAWVAIDWLGVYDGSYWGNMQNSVTFSGDVCNGIINSIVFAFVVTWIAVFQGYNCEPTSEKISHATTKTVVYASLAVLGLDFILTALMFGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM38</accession>
    <name>A0A481QM38_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase H</fullName>
            <ecNumber evidence="6">2.1.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA m(4)C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">rRNA (cytosine-N(4)-)-methyltransferase RsmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">rsmH</name>
        <name evidence="7" type="ORF">C3B55_00584</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10287"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74506"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.199" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00584"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="PTHR11265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004486">
        <property type="entry name" value="MraW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="SSF81799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00006">
        <property type="entry name" value="TIGR00006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="25"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="303" mass="33211" checksum="6D4BDB6454DCE289" modified="2019-06-05" version="1">MLAEAVEALAIRADGYYLDGTFGSGGHSRLILSQLGLNGKLLGFDKAPQAIVAGQALAAEDGRFVVVQRSFAELGTEVAGRSMVGKVAGILFDLGVSSSQLDNAEHGFSFMKDGPLDMRMDPTRGISAAQFIATAPVEEITRVFREYGEERFASRMARAVVERRNIYPIKRTVDLAEVLKGANPAWEKGKNPATRAFQGLRIHINNELGDLEAGLEAALEALEVGGRLVVISFHSLEDRMVKLFMRRLVKGEPNNLPRNLPIRFEAFAPKIKIHGKAKFASEAELQINHRSRSAVMRVAEKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM89</accession>
    <name>A0A481QM89_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucose-1-phosphate thymidylyltransferase</fullName>
            <ecNumber evidence="8">2.7.7.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rmlA1</name>
        <name evidence="10" type="ORF">C3B55_00841</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>alpha-D-glucose 1-phosphate + dTTP + H(+) = diphosphate + dTDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:15225"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5 8">Belongs to the glucose-1-phosphate thymidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.24" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00841"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008879">
        <property type="term" value="F:glucose-1-phosphate thymidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045226">
        <property type="term" value="P:extracellular polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02538">
        <property type="entry name" value="G1P_TT_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="dTDP_sugar_isom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005907">
        <property type="entry name" value="G1P_thy_trans_s"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43532">
        <property type="entry name" value="PTHR43532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01207">
        <property type="entry name" value="rmlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="426"/>
        </location>
    </feature>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="7">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="476" mass="53690" checksum="F2F9F2639234A371" modified="2019-06-05" version="1">MLKRKGIILAGGSGTRLHPTTLAISKQLLPVYNKPMIYYPLTTLMLADIRDILIISTPQDTPRFEQLLGDGSNWGLNISYTVQNSPDGLVQAFLIGKDFIGKDLSVLVLGDNIHHGHNFNKLLKKATRHQDGASVFAYQVNDPERYGVVEFDAHGRAISLEEKPLKPKSNYAITGLYFYDQDVIEIARNIKPSQRGELEITDLNRIYMERGKLSVEIIGRSYAWLDTGTHDSLFEASSFIATLERRQNFKIACPEEIAFRKNWIDAEQLERLATPLLKNSYGQYLKCLMTEKIHSMKHSELAISGVVLIEPRIFSDKRGFFFESFNKKQFESAIGRSVKFVQDNHSHSMRNVLRGLHYQVCQPQGKLVRVVSGEIFDVAVDIRKGSATFGQWVGEVLSAENKRQLWVPEGFAHGFVVLSESAELLYKSTNYYAPAYEHCIAWDDPSLAIKWPIIGTPTLSEKDTQGQLLSAAKVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM93</accession>
    <name>A0A481QM93_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00590"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="40" mass="4393" checksum="E43D16315B5BDA04" modified="2019-06-05" version="1">MLTLLRLAVLASSIIMRVAKSRQMTHLLSNITAKIAASLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMB3</accession>
    <name>A0A481QMB3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-Glc:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,3-glucosyltransferase WfgD</fullName>
            <ecNumber evidence="3">2.4.1.305</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">wfgD_2</name>
        <name evidence="3" type="ORF">C3B55_00852</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.305" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00852"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="356" mass="41320" checksum="09774ACA3F1483DC" modified="2019-06-05" version="1">MSNTLISVIMATFNHASYVKEAITSVLNQHGELEFLIADDGSTDGTREIIESMIDPRIRFFPHYKNRGACTVTNELIALSKGQFIALINSDDFWCDNNKLAYQLQILLKNPQLGATFSQAYFIDKHGNHISKNRLTFGSVFEQQNRSRGKWLRLFFDQGNCICHPTILIRRACYNNIGVYNNNLRQLPDFDMWVRLVKKYQIYISEQQLISFRVLPRHSASSQTVANSIRSMNEHYLIATSFFQNVRAQELKDGFCDLLKYPNIPSDIHLNIEKTLLFFMPNKAFSKVYEIVGILKLNRLLSSDRHREVLEKDYKINASWYHRKMQEVAVLRPKFSAVVSNNIANLKSVYLRLFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMD1</accession>
    <name>A0A481QMD1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Outer membrane porin F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">oprF</name>
        <name evidence="12" type="ORF">C3B55_00861</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00861"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008722">
        <property type="entry name" value="OprF_membrane_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05736">
        <property type="entry name" value="OprF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0626">Porin</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019758509" evidence="10">
        <location>
            <begin position="25"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="11">
        <location>
            <begin position="204"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="323" mass="34574" checksum="E9B697FB9B9D5E6F" modified="2019-06-05" version="1">MKLKNTLSFAFGAVIVAAAFSALAQGQGAVEGQLFYEKQYNNSVTHIEDGFHPFAGIGYFLTDDVSLNLSYNNTNYSHSGNGIGNNKIKGDASSVTAQYHLGQANVNSLRPYIEGGFAHQSHTNITTHGHTGRNQTTLAIAGAGAKYYFTNNLFARAGVEADYAIDNGKLDYAALVGLGVNFGGNASAVDSISTPVHTSEPEAPVAQAIRVELDVKFDFSKSVVNYNSYGDVQRIANLMKQYPHITIVLKGHTDSVGSDAYNQKLSQHRADAIKQLLVEDGIAPGRVSSVGYGKLRPVADNSTEAGRAINRRVEATIEVQANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMH0</accession>
    <name>A0A481QMH0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00814"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="72" mass="8320" checksum="B82D1E4F576F2CB6" modified="2019-06-05" version="1">MVTISMYYEILSLLYCIVIKRKTIDDAVRLKLIATLKLCDELSLLLAGLLIYKKTKLLYGGHKYYEVCACRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMH6</accession>
    <name>A0A481QMH6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GTPase Era</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 12" type="primary">era</name>
        <name evidence="12" type="ORF">C3B55_00556</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00556"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00367">
        <property type="entry name" value="GTPase_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTP-bd_Era"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="PTHR42698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00436">
        <property type="entry name" value="era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51713">
        <property type="entry name" value="G_ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Era-type G" evidence="11">
        <location>
            <begin position="8"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="10">
        <location>
            <begin position="199"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="8">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="8">
        <location>
            <begin position="42"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="8">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="8">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="8">
        <location>
            <begin position="155"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="300" mass="34356" checksum="094E6E628F7765E0" modified="2019-06-05" version="1">MTNLTIKRCGYAAIVGRPNVGKSTLLNHILGQKLAITSRKPHTTRHNMLGIKTEDNVQTVYIDTPGIHRGRKKAINRYMNKTALAALKDVDVVIFMIDRNKWTDEDQIVLECVRHVTGQLIVTLNKTDRLEVRAELVPHISWLQEHLPNAQIIPISAQSGHNLDVLKRMIAKYLPENEYYFPEDEITDKSSLFLVAEMIREKIIRQIGAEVPYQITVKTEEFKQQGKVLHVHALIFVERDGQKKIIIGDKGERIKRIGTEARKDMELLFTSKIMLNLWVKVSSGWSNDAHTLRYLGYGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMI3</accession>
    <name>A0A481QMI3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00476"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="58" mass="6587" checksum="B5B0433B5605F0A0" modified="2019-06-05" version="1">MLGLMHSHNNRLAIVFVNPVWVHVEFVFANYIPNLHKQFAMAGIKMLFAVAYQASVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMI4</accession>
    <name>A0A481QMI4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lipoprotein-releasing system transmembrane protein LolC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">lolC_1</name>
        <name evidence="12" type="ORF">C3B55_00708</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC-4 integral membrane protein family. LolC/E subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00708"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011925">
        <property type="entry name" value="LolCE_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02212">
        <property type="entry name" value="lolCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="11">
        <location>
            <begin position="35"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="10">
        <location>
            <begin position="309"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="422" mass="46274" checksum="ED3EE4236FB438BB" modified="2019-06-05" version="1">MFIQRYQLMFRPLSIFIGTRYISAKRRNRFISFISMTSMIGLALGVLAMIVVLSIMNGFQREMRIRVLGIVPHAIIIGVNPINDWRPVAKAALKNLQVTAAVPFTQIDGMFSYRGMMQPIEISGVDPAQESTVSNVVQHIVRGKLEDLKPGEFGVVISDITARRFRLNIGDKLIFIVPEISTVLGGIVPHLQRLSVVGIFDVSTELYGSIALINIADAAVMRHWQSGQVQSVRLALKDIYLAPKVSRDIVARLGTGYMSDDWTHTQNSLFSAMKMEKTMIGLLLLMIIVVAAFNIIATLSMVVNDKSTDIAVLCTIGATPRQIMAIFMVQGTVIGTVGVLIGSLLGVITALNLSQLESWLESVTGQHIFSSGVYLVSNLPSELQGSDVLLICTVGFVLSFIATIYPAYIAAKIEPAHALRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMI6</accession>
    <name>A0A481QMI6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Major cold shock protein CspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cspA</name>
        <name evidence="4" type="ORF">C3B55_00919</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00919"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="131"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="200" mass="22433" checksum="9B1255474B3B5D12" modified="2019-06-05" version="1">MLKIVHLLTGVIALLLSLIPSMQPEKLPYLEQQDALYLVLFGLINMTLAPMIHYWHKSTRHQLQHLVSALLVLTVIVQAIFLLVPIPEVGGHRTILVSLMLAIVAIVLHIAINLSSSSYVPVVQHYNITNRDTGTVKWFNTSKGFGFISRDSGDDIFVHFRAIRGDGQRILIEGQRVEFSIINRDKGLQAEDVITVLPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMK0</accession>
    <name>A0A481QMK0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome-recycling factor</fullName>
            <shortName evidence="3">RRF</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Ribosome-releasing factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">frr</name>
        <name evidence="5" type="ORF">C3B55_00768</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the RRF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00768"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00520">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00040">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="PTHR20982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="SSF55194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00496">
        <property type="entry name" value="frr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRF" evidence="4">
        <location>
            <begin position="22"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="185" mass="20703" checksum="BACBF7EB2C711A5A" modified="2019-06-05" version="1">MIHGIKKDAQARMQKSMEALSYALSQIRTGKAHPSILGNIMVSYYGVNTPLSVVANITIKDSRTLQVVVFERHMLEVVNKAIQGAGLNLNPNNLGELLLISMPALTEETRKCCTKQARSAAEYARVAVRNIRRDALGDFKKLVKDKKVSEDEERRAISYIDKLTKDAEIQITKTTEEKEKDLMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMK3</accession>
    <name>A0A481QMK3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold shock protein CapB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">capB_1</name>
        <name evidence="4" type="ORF">C3B55_00745</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_002554837.1">
        <property type="nucleotide sequence ID" value="NZ_CP026512.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A481QMK3"/>
    <dbReference type="GeneID" id="61879477"/>
    <dbReference type="GeneID" id="64467183"/>
    <dbReference type="GeneID" id="67382191"/>
    <dbReference type="KEGG" id="pade:C3B55_00745"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="69" mass="7727" checksum="659F8F3EF217678B" modified="2019-06-05" version="1">MSNRQTGTVKWFNDEKGFGFITPQSGDDLFVHFKAIQSDGFKSLKEGQQVSFIATRGQKGMQAEEVQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QML7</accession>
    <name>A0A481QML7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase</fullName>
            <shortName evidence="7">UDP-N-acetylglucosamine acyltransferase</shortName>
            <ecNumber evidence="7">2.3.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">lpxA</name>
        <name evidence="9" type="ORF">C3B55_00759</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a (3R)-hydroxyacyl-[ACP] + UDP-N-acetyl-alpha-D-glucosamine = a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:67812"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="ChEBI" id="CHEBI:173225"/>
            <dbReference type="EC" id="2.3.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transferase hexapeptide repeat family. LpxA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.129" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00759"/>
    <dbReference type="UniPathway" id="UPA00359">
        <property type="reaction ID" value="UER00477"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008780">
        <property type="term" value="F:acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03351">
        <property type="entry name" value="LbH_UDP-GlcNAc_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00387">
        <property type="entry name" value="LpxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029098">
        <property type="entry name" value="Acetyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037157">
        <property type="entry name" value="Acetyltransf_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010137">
        <property type="entry name" value="Lipid_A_LpxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43480">
        <property type="entry name" value="PTHR43480"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13720">
        <property type="entry name" value="Acetyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000456">
        <property type="entry name" value="UDP-GlcNAc_acltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01852">
        <property type="entry name" value="lipid_A_lpxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Acetyltransf_11" evidence="8">
        <location>
            <begin position="176"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="258" mass="27893" checksum="741660B729217CA3" modified="2019-06-05" version="1">MSLIDSRAIIESSAVLAADVKVGPWSIIGAGVEIGEGTVIGSHVILKGPTFIGKHNRIYQFSSVGEDTPDIKYKGEETRLVIGNYNIIREGVTIHRGTVQDRAETTLGDHNLIMAYAHIGHDSVIGNHCILVNNTALAGHVHVEDRATLSGFTLVHQYCHIGAYSFSGMGTAIGKDVPAFVTVFGNPAEARSMNFEGMRRCGFSEDAIHALRRAYKVVYRQGLTVEQALIALTEPAALFSEVAVFRDSIQASTRGITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMM5</accession>
    <name>A0A481QMM5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Co-chaperone protein HscB homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">hscB</name>
        <name evidence="6" type="ORF">C3B55_00961</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward HscA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with HscA and stimulates its ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the HscB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00961"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00682">
        <property type="entry name" value="HscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036386">
        <property type="entry name" value="HscB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009073">
        <property type="entry name" value="HscB_oligo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="PTHR14021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07743">
        <property type="entry name" value="HSCB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47144">
        <property type="entry name" value="SSF47144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00714">
        <property type="entry name" value="hscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="3"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="182" mass="21283" checksum="AFFCFC1114D935E4" modified="2019-06-05" version="1">MPCYFALFDLQQSFWLDLEQLATRYRELALEVHPDLFVGASEYKQRLVLERSASLNEAYQTLKSPTKRARYLLAMNGDKLPIEVTVHDPDFLMQQMQWHEELEDLQSETNLAGLAVFKHTLKVAQNELNKSFAACWDNAAQRQHAECLMRRMQFLDKLTHEVHQLEEHLDDELDAAIHTRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMM6</accession>
    <name>A0A481QMM6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chaperone protein HscA homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">hscA</name>
        <name evidence="7" type="ORF">C3B55_00960</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00960"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10236">
        <property type="entry name" value="HscA_like_NBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00679">
        <property type="entry name" value="HscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042039">
        <property type="entry name" value="HscA_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010236">
        <property type="entry name" value="ISC_FeS_clus_asmbl_HscA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01991">
        <property type="entry name" value="HscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="537" mass="57104" checksum="AD961030AC9B7524" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMN5</accession>
    <name>A0A481QMN5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Queuine tRNA-ribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">tgt</name>
        <name evidence="7" type="ORF">C3B55_00971</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>7-aminomethyl-7-carbaguanine + guanosine(34) in tRNA = 7-aminomethyl-7-carbaguanosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:24104"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:10342"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:58703"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82833"/>
            <dbReference type="EC" id="2.4.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.29" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00971"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:queuine tRNA-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101030">
        <property type="term" value="P:tRNA-guanine transglycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TGT" evidence="6">
        <location>
            <begin position="14"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="92"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding" evidence="5">
        <location>
            <begin position="246"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding; important for wobble base 34 recognition" evidence="5">
        <location>
            <begin position="270"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="374" mass="41485" checksum="2E92825F3A8001CA" modified="2019-06-05" version="1">MSHMSFKLLAVDGKARRGRLTFPRGNVETPAFIPVGTYGTIKGILPRDIVTTGTEIILSNTFHLWQRPGTAVIKRHGDLHDFMKWQGPILTDSGGFQVFSLGAIRKIKEEYVTFISPVDGSKVFISPEESMQVQRDLGSDIVMIFDECTMYPADEGVARISMELSLRWAQRSKSAHGDNIAALFGIVQGGMHENLRKLSLEGLDKIGFDGLAIGGLSVGEPKHEMIAVLDYLPGLMPANKPRYLMGVGKPEDLVEGVRRGIDMFDCVIPTRNARNGYLFIDTGVLRIRNALHRHDDSLLDSSCDCYTCQNFSRAYLHHLNKCGEILGSMLNTIHNLRYYQVLMASLRDAIQQGTLTAIVAAFYAKRGLTVPPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMP4</accession>
    <name>A0A481QMP4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase Obg</fullName>
            <ecNumber evidence="5">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein Obg</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">obg</name>
        <name evidence="8" type="ORF">C3B55_00982</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00982"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="PTHR11702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002401">
        <property type="entry name" value="GTP_bd_Obg/CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="SSF82051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="7">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="6">
        <location>
            <begin position="160"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="166"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="191"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="213"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="283"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="314"/>
            <end position="316"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="356" mass="38832" checksum="B32EDF182FEF19E7" modified="2019-06-05" version="1">MQFVDEAFIRVKAGDGGNGCMSFRREKFIENGGPNGGDGGNGGSIYIIADANLNTLVDYRYTRYFDASCGSNGGSANCTGKKGEHLVLRVPVGTTIIDSSSQEVIGDLTRSGQNLMIVQGGWHGLGNTRFKSSTNRAPRQTTLGKPGGQRDLKLEMKVMADVGLLGLPNAGKSTLIRSVSDAKPKVADYPFTTLIPNLGVVKVDSWKSFVIADIPGLIAGASDGVGLGMRFLKHLSRTRLLLHLVDIAPLDEACASDAAEVIINELTKFSPALAQRDRWLVLNKCDQILEEEHEERVKEIVNHIEWKGPVYVISAVLKKGTERLIFDIVRYLENCAERLTNDQVYKTKLVGLRRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMR7</accession>
    <name>A0A481QMR7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00847</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00847"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="48" mass="6020" checksum="5AF50FC77DCC8125" modified="2019-06-05" version="1">MFFYQHGQNFILRLWQPFFHVVDLFNKRVISVNFFADQIKFHDSRNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMS0</accession>
    <name>A0A481QMS0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fadD</name>
        <name evidence="3" type="ORF">C3B55_00901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00901"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="31"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="470"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="562" mass="62076" checksum="075708E895FAB982" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMT1</accession>
    <name>A0A481QMT1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00913"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="77" mass="8889" checksum="552A822B60EB9C91" modified="2019-06-05" version="1">MVKLLCNKIQKRLHKIKESKILRKLQQPRKLPKKRQRKNPLLMSLKMAQEAARKSAATLATSWLPNIVMNALTVLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMT6</accession>
    <name>A0A481QMT6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00813</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00813"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018637">
        <property type="entry name" value="DUF2059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09832">
        <property type="entry name" value="DUF2059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019752688" description="DUF2059 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2059" evidence="2">
        <location>
            <begin position="91"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="171" mass="19159" checksum="69BFC70040A3CF19" modified="2019-06-05" version="1">MTCFRAICIAVILVYASRQVFADIASHNASAEAFLTLVHAYKLGAPVYIQVQQMFAQRFEQTRAPASKRYILDSYQAKAHDVIDQAIGWSKLKPEMIKLYTANFSESELNDLVAFYQSVLGKKVLEKMPELTQQSAQITQAKLESAVSAVNELLERMTNELAPKSDVPVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMU2</accession>
    <name>A0A481QMU2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="4">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fabG</name>
        <name evidence="5" type="ORF">C3B55_00878</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 4">Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00878"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011284">
        <property type="entry name" value="3oxo_ACP_reduc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01830">
        <property type="entry name" value="3oxo_ACP_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 4" id="KW-0521">NADP</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="62"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="154"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="247" mass="26047" checksum="EC5229036FCA5152" modified="2019-06-05" version="1">MSLQGRVALVTGASRGIGQAIALELGRHGAVVIGTATSVLGAERIATVLKENSIQGTGFELNVTSDESVSVILAKITAQFGAPAILVNNAGVTHDNLMIRMKDDEWHDVIDTNLNSLFRLSKGVLRDMTKMRWGRIINIGSVVGAMGRTGQVNYAAAKAGLEGFSRALAREVGSRSITVNSVVPGFIDTDMTRKLSEAQRQVLLAQIPLGRLGHAQEIANVVTFLASDGAVYVTGASIPVNGGMYMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMU3</accession>
    <name>A0A481QMU3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bifunctional chorismate mutase/prephenate dehydratase</fullName>
            <ecNumber evidence="8">4.2.1.51</ecNumber>
            <ecNumber evidence="7">5.4.99.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Chorismate mutase-prephenate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">p-protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">pheA</name>
        <name evidence="23" type="ORF">C3B55_00696</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the Claisen rearrangement of chorismate to prephenate and the decarboxylation/dehydration of prephenate to phenylpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:21648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="4.2.1.51"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="8"/>
    <dbReference type="EC" id="5.4.99.5" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00696"/>
    <dbReference type="UniPathway" id="UPA00120">
        <property type="reaction ID" value="UER00203"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008242">
        <property type="entry name" value="Chor_mutase/pphenate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
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        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010957">
        <property type="entry name" value="G/b/e-P-prot_chorismate_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001500">
        <property type="entry name" value="Chor_mut_pdt_Ppr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="SSF48600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01807">
        <property type="entry name" value="CM_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00857">
        <property type="entry name" value="PREPHENATE_DEHYDR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="20">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Prephenate dehydratase" evidence="21">
        <location>
            <begin position="95"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="22">
        <location>
            <begin position="284"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="site" description="Essential for prephenate dehydratase activity" evidence="19">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00014401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR001500-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="QAX82019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="365" mass="41424" checksum="9D91AD799D3C89E5" modified="2019-06-05" version="1">MSEQTIKDFRVRIDSLDDRVLELISERAFCAQEVARAKIASVATGEVPIFYRPEREIQVLKRMIKRNKGPLSNEEIVWLFREIMSSCLALEQPLKVAYLGSEGNFTQLAAMKYFGHAVISKPIAAIDELFCDVVAGVVKFGVVPISNSTEELVDHMLDSFFERNMVICGEVDLRIQHHLLVCGNTKTNRTTSVYSHTQSLVQCSKWLYANYPNVERVTWSSDAEAAKLAQSNEWNSASITGDMVESPYRLTSMVEKIEDLQDNFTRFLIIGSEEIPPTGDDKTSIIVSMSNRPEALYKLLVPFYEHRIDLTRIEVRLARSSKSTYMFFVDFIGHCRDSLVKDVLEQVSHEALTFKILGSYPKAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMU5</accession>
    <name>A0A481QMU5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-binding protein HU-beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hupB</name>
        <name evidence="5" type="ORF">C3B55_00602</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="90" mass="9211" checksum="FB1ED57C9548224E" modified="2019-06-05" version="1">MNKSELIDAIAISADISKAAASRALNAVIESVTGALKAGDSVILVGFGTFSVTDRRARTGRNPQTGKALQIAAAKKPAFKAGKALKEAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMW3</accession>
    <name>A0A481QMW3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Inner membrane protein YpjD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ypjD</name>
        <name evidence="3" type="ORF">C3B55_00944</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00944"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="2">
        <location>
            <begin position="44"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="270" mass="29694" checksum="3CB02ED532F6E8F1" modified="2019-06-05" version="1">MVLLSPNLLSSFAAAILYTAATFYQGANLAQGNKADKNVLIGLGVVALLAHAMSLFTHLITPAGLGLDFFNAASLIAAAVIALTMLACYCIPVENLLVLLFPLGMLTVLLAQLTPTGTVQMVNEKPGILAHILLSILAYGVFTIAVFQALLLLLQDYQLKNKHPSRLIKNFPPLQTMESLLFQFLLVGWSLLSLSLISGWLFVENLFAQHLMHKTMLACLAWAVFSVLLWGRNRLGWRGHKAIRWTLAGFCLLMLAYFGSKLVREYILHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMX0</accession>
    <name>A0A481QMX0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">6-carboxy-5,6,7,8-tetrahydropterin synthase</fullName>
            <ecNumber evidence="8">4.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">queD</name>
        <name evidence="11" type="ORF">C3B55_00625</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H(+) + triphosphate</text>
            <dbReference type="Rhea" id="RHEA:27966"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="EC" id="4.1.2.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PTPS family. QueD subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00625"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070497">
        <property type="term" value="F:6-carboxy-5,6,7,8-tetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PTHR12589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006113">
        <property type="entry name" value="PTP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03367">
        <property type="entry name" value="queuosine_QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006113-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006113-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="126" mass="14674" checksum="655F0DF281722CD9" modified="2019-06-05" version="1">MEIFKEFTFESAHRLPYVPESHKCWRLHGHSFKVAIHLVGNLDYNTGWLRDFSNIKAIFKPLHERLDHNYLNDIPGLENPTSEVLAQWIWTELKPTLPELSAVRIYETCTSGCIYRGNIATCKSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMZ7</accession>
    <name>A0A481QMZ7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome bo(3) ubiquinol oxidase subunit 1</fullName>
            <ecNumber evidence="8">7.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cytochrome o ubiquinol oxidase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Oxidase bo(3) subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Ubiquinol oxidase polypeptide I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquinol oxidase subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">cyoB</name>
        <name evidence="21" type="ORF">C3B55_00977</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a ubiquinol + n H(+)(in) + O2 = 2 a ubiquinone + n H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30251"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="EC" id="7.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a quinone</name>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>heme o</name>
            <dbReference type="ChEBI" id="CHEBI:24480"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterooctamer of two A chains, two B chains, two C chains and two D chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.3" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00977"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016682">
        <property type="term" value="F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014207">
        <property type="entry name" value="Cyt_c_ubiqinol_oxidase_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02843">
        <property type="entry name" value="CyoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="18" id="KW-0349">Heme</keyword>
    <keyword evidence="18" id="KW-0408">Iron</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="11 18 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="423"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="494"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="610"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="COX1" evidence="20">
        <location>
            <begin position="39"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00014691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="QAX82276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="668" mass="74586" checksum="E4CD8B46175CD71D" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN13</accession>
    <name>A0A481QN13_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">3-oxoacyl-[acyl-carrier-protein] synthase 2</fullName>
            <ecNumber evidence="3 10">2.3.1.179</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">fabF</name>
        <name evidence="14" type="ORF">C3B55_00876</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor. Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(9Z)-hexadecenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(11Z)-octadecenoyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:55040"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10800"/>
            <dbReference type="Rhea" id="RHEA-COMP:14074"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:83989"/>
            <dbReference type="ChEBI" id="CHEBI:138538"/>
            <dbReference type="EC" id="2.3.1.179"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 12">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.179" evidence="3 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00876"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="13">
        <location>
            <begin position="6"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX82185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="413" mass="43459" checksum="D3EA8EDF21A672D2" modified="2019-06-05" version="1">MSRRRVVVTGMGMLSPLGTDVPSSWQGVLAGHSGISLIKHTDFSAYSTRFGGSIKGFNVAEYLSIKESRKLDLFIQYGLAAGFQAVRNSGLEITDANRERIGVVMSSGIGGLTNIEETSRTLHNAGPRRISPFFVSGSIINMISGFLSIHLGAQGPNYAITTACTTGTHCIGMAARNIAYDEANVMIAGGAEMAACGLGIGGFSASRALSTRNDEPTRASRPWDRGRDGFVLSDGAGALVLEELEHAKMRGATIYAELIGFGMSGDAYHMTSPPSDGAGAARCITNALRDAKINANQVQYINAHGTSTLTGDLAEVKAIKSVFGEYAYKLAVSSTKSMTGHLLGAAGAVEAIFSMMAIKDQIAPPTINLDEPDAGCNLNFVPHEAQPMLIDVAISNSFGFGGTNGSLVFRRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNB3</accession>
    <name>A0A481QNB3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Sec translocon accessory complex subunit YajC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">yajC</name>
        <name evidence="11" type="ORF">C3B55_00970</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The SecYEG-SecDF-YajC-YidC holo-translocon (HTL) protein secretase/insertase is a supercomplex required for protein secretion, insertion of proteins into membranes, and assembly of membrane protein complexes. While the SecYEG complex is essential for assembly of a number of proteins and complexes, the SecDF-YajC-YidC subcomplex facilitates these functions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Part of the SecDF-YidC-YajC translocase complex. The SecDF-YidC-YajC translocase forms a supercomplex with SecYEG, called the holo-translocon (HTL).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the YajC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00970"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003849">
        <property type="entry name" value="Preprotein_translocase_YajC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33909">
        <property type="entry name" value="PTHR33909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02699">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01853">
        <property type="entry name" value="YAJCTRNLCASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01323">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00739">
        <property type="entry name" value="yajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0811">Translocation</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="112" mass="12481" checksum="F77F5ADB4339C46F" modified="2019-06-05" version="1">MSFFIFNVMADSAVPTVVSYMGGGFEWIFLVGFLIIFYLIIWRPQAKRVKEQKKLLGSLQKGDEVVTTSGIVGKIIKISDDFVVLEVSKTIEMKFQKSAIATTLIKGTLEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-08-12" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNJ7</accession>
    <name>A0A481QNJ7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable transcriptional regulatory protein C3B55_00916</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pmpR</name>
        <name evidence="5" type="ORF">C3B55_00916</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the TACO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00916"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="PTHR12532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="SSF75625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01033">
        <property type="entry name" value="TIGR01033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="248" mass="27214" checksum="45E13D3BAC467BFA" modified="2019-06-05" version="1">MAGHSKWTNIKHRKEHQDAKKGKIFTKLVRELTVAVRHGGSDPCYNPCLRIALDKAFRVNMRRDIIDRAIASAIGSTNTSNMMELSYEGYGPQGVAVIVECITDNRNRTAAAVRHIFSKYGGTLGTDGSVAYLFERKGQITFAPGVSEDALMEAAIKASANDVVINEDNSIVVFVSFTSFYSVYNYLEVSGFKVATAEIVMLSTVSAKVDLDNAQKVLKMLDMLEGLDDVQNVYSNANVSRSAVKQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNS4</accession>
    <name>A0A481QNS4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfotransferase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00844"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008476">
        <property type="term" value="F:protein-tyrosine sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006478">
        <property type="term" value="P:peptidyl-tyrosine sulfation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026634">
        <property type="entry name" value="TPST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788">
        <property type="entry name" value="PTHR12788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="348" mass="40480" checksum="2036DBA0333340AD" modified="2019-06-05" version="1">MSEQIKQAFIIGTGRCGTTWLGQMLNSNPVLCVPPEIQLLFEYSGNGNRLYEEYLLTKEQGLDTNQLISIIERGCPHNLYQFFDYPAFCRQHTTPKRSLREFVTAFYSAIAKSHGKLWLIEQTPWYGQRIDLATSLFPDAQFIHVVRDGRDVALSFSRTTWWHQSTRLNLSRWQREVKKIALDAQYFLKPEAYLEVKYENLVADTAVELKKICQFLGVNFDIAMLDPHGFIDYNQFCKFDLDQVSSQAYSVWYKQKNQTVFADNVEAWRKNDIAFDRSLPSEISSWLSYYGYPIEFTDEAETANLHLREYSLNALEQDNIEKSQHIQTLEQSVADYVQHTHIGNVKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QJY5</accession>
    <name>A0A481QJY5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="3">DAP decarboxylase</shortName>
            <shortName evidence="3">DAPDC</shortName>
            <ecNumber evidence="3 4">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 7" type="primary">lysA</name>
        <name evidence="7" type="ORF">C3B55_00037</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="3 4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00037"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3 4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 4 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="5">
        <location>
            <begin position="33"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="6">
        <location>
            <begin position="35"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="3">
        <location>
            <begin position="273"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="415" mass="46064" checksum="786D69B7DEBF3B7E" modified="2019-06-05" version="1">MDAFNYRDGELFAEGVALSTLAERFGTPTYVYSRAHIEAQYYSFTDPLEGVSYMVCYAVKANSNLSILNILARLGAGFDIVSRGELERVLAAGGKPNKIVFSGVGKSREDMRYALEVGVYCFNIESKDELKRLQVVAAEMGVCAPISLRVNPDVDAKTHPYISTGLKKNKFGIAITDAEDLYIHATQLANLEVLGINCHIGSQLTTLLPFLDAFDRLLILTDRLSKCNIYLKHIDFGGGLGVRYHNEKPPQITEYIQAVRKRTKGRGLTLIFEPGRYIVANASVLLTQVEYLKHTKHKNFAIVDAAMNDLIRPALYEAWMDVTAVTPRVGVSRTYDIVGPICETGDFLAKDRKLVMEEGDLLAIHSTGAYGFAMSSNYNTRGRAAEVMVDGEQAFEVRRREMVAELYASESLLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK59</accession>
    <name>A0A481QK59_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="4 11">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">hemB</name>
        <name evidence="13" type="ORF">C3B55_00046</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="4 11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00046"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="PTHR11458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="336" mass="37052" checksum="1350022188C520F4" modified="2019-06-05" version="1">MSFTLANRLFPATRLRRNRCDDFSRRLVRETVLTTNDLILPVFVLDGENCREAVASMPGVERLTIDLLLKEVANWVELGIPALAIFPVTPSKLKSLDAAEAWNPEGITQRAARTLRKYCPELGVITDVALDPFTTHGQDGILDKDGYVQNDITVDALVKQALSHATAGAQVVAPSDMMDGRIKAIRDALELAGHVNVRIMAYSVKYASAYYSPFRDAVGSALNLGKAHKISYQMDPANRQEAMHEVAADLAEGADMVMVKPGMSYIDILYLVKKEFKVPTFVYQVSGEYAMHMAAIQNGWLSERVILESLTAFKRAGADGILTYFAARAAQLLREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKE1</accession>
    <name>A0A481QKE1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Transketolase</fullName>
            <ecNumber evidence="5 10">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tktA</name>
        <name evidence="12" type="ORF">C3B55_00101</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit. Can also utilize other divalent metal cations, such as Ca(2+), Mn(2+) and Co(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="10">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="5 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00101"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005478">
        <property type="entry name" value="Transketolase_bac-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033247">
        <property type="entry name" value="Transketolase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522">
        <property type="entry name" value="PTHR43522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00232">
        <property type="entry name" value="tktlase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="665" mass="72999" checksum="6FB24CF0A1B3CCD3" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKH9</accession>
    <name>A0A481QKH9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00043"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="48" mass="5673" checksum="F2F1ECC2F8A8BACC" modified="2019-06-05" version="1">MLSFYVFFILLLSVLNTAYFLTTCMLIKLVIVIRHAKRLVVYFLLQTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKL8</accession>
    <name>A0A481QKL8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Molybdopterin biosynthesis protein MoeB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00165"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="48"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="137" mass="15100" checksum="30670948EAB8C34A" modified="2019-06-05" version="1">MVDQLIVFITTNYLLVAAFIILLTLLIAYEISLSGRSLSTSELIMLVNKNKDVIVDIRPVKDFTVGHIFGAINIPHDKLIARLVELEKYKAKTIILVDAQGRYAGINTSEMLKAGFIVAKLSGGISSWKANNLLLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKN4</accession>
    <name>A0A481QKN4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylenetetrahydrofolate reductase</fullName>
            <ecNumber evidence="10">1.5.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">metF</name>
        <name evidence="11" type="ORF">C3B55_00097</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:19821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19817"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.20" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00097"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106312">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106313">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADPH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004620">
        <property type="entry name" value="MTHF_reductase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00676">
        <property type="entry name" value="fadh2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="6 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 10 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="281" mass="31586" checksum="28B2D389CA293C39" modified="2019-06-05" version="1">MFQNRRYSFEFFPTKTDAGHKKLMTIAKQLASYNPDFFSCTYGAGGSTRNSTMKTVLQLENEIKISTAPHLSCIGDSKANLRSLLTQYKQAGIKRIVALRGDLSLDMSMSSGELCYANDLVSFIRQESGDHFHIEIAAYPEMHPQARNFEDDLKNFIRKANSGANSAITQYFFNTDSYFYFIERILAMGVDIPIVPGIMPITNYNKLARFSKACGAEIPRWICKQLEVYGEDVKSTKAFGEQVISNMCEQLLQGGAPGLHFYTMNLAEPSQAILDSLKRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKT1</accession>
    <name>A0A481QKT1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="5">2.5.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Dimethylallyl diphosphate:tRNA dimethylallyltransferase</fullName>
            <shortName evidence="5">DMAPP:tRNA dimethylallyltransferase</shortName>
            <shortName evidence="5">DMATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Isopentenyl-diphosphate:tRNA isopentenyltransferase</fullName>
            <shortName evidence="5">IPP transferase</shortName>
            <shortName evidence="5">IPPT</shortName>
            <shortName evidence="5">IPTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">miaA</name>
        <name evidence="9" type="ORF">C3B55_00228</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5 7">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5 6">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5 8">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00228"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="PTHR11088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="5">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="5">
        <location>
            <begin position="160"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="5">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="5">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="319" mass="35613" checksum="E5CA47C2BF9F70F7" modified="2019-06-05" version="1">MSVLPPAIFLMGATATGKTHLAIELTKVLPCELISVDSAMVYRDMDIGTAKPSKEFLAQYPHCLIDIIDPSESYSAADFRTDALAAMAEITSRGNIPLLVGGTMLYYKALQEGLADAPADAIVRTELKEEAVRHGWQALHNQLAVVDPVSAARIHPNDSQRLTRALEVWRVSGQTMTEHRLKQAAKNTYACASGRVQLPYKVVNLAITSVNRQVLHERITQRFAIMLEQGFIGEVIALHSRSDLHSGLPAIRAIGYRQVWDYLDNKLSFTEMHERCIIATRQLAKRQFTWLRNWSNLYWLDSDNLSCALKYLGTVSTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKU2</accession>
    <name>A0A481QKU2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ADP compounds hydrolase NudE</fullName>
            <ecNumber evidence="3">3.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nudE</name>
        <name evidence="3" type="ORF">C3B55_00150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00150"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="41"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="188" mass="21286" checksum="462A7F09EA3C214C" modified="2019-06-05" version="1">MRQKPTIFDREIVASSRLFFIEEVQLRFSNGIERTYERLVGRGAGCGAVMIVAMIDTYYALLVEEYCAGTDKYEFSLPKGLIEPGENILAAANRELREEAGYSARQLEHITELSLSPGYMSHKIQVVLATDLYEERAEGDEPEFMRVERINLRDLSMLAQNAQFSEGRALAALYLVRDLLTQYGAFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKU7</accession>
    <name>A0A481QKU7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP synthase subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP synthase F(1) sector subunit delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">F-type ATPase subunit delta</fullName>
            <shortName evidence="7">F-ATPase subunit delta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">atpH</name>
        <name evidence="8" type="ORF">C3B55_00008</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein is part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ATPase delta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00008"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="PTHR11910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="SSF47928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0139">CF(1)</keyword>
    <keyword evidence="2 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="3 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="178" mass="19450" checksum="B921308BD083717B" modified="2019-06-05" version="1">MVELKTLARPYSKAAFEYAYAHQQLTSWSTMLSLAAAVAQDNTMQCLFHDASLTSADKAAACIEVCGDKLNLKAQNFIYIVAENNRLQILSEISILFDQYKAEEEKSVNVAVTSVFALNQDQQGKLAKALTTRLNREVHLQLSEDASLIGGLIIRAGDVVIDGSIRGKISKLAEALKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKX3</accession>
    <name>A0A481QKX3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rpsJ</name>
        <name evidence="6" type="ORF">C3B55_00276</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00276"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_S10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_S10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_S10_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="PTHR11700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="SSF54999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_S10" evidence="5">
        <location>
            <begin position="7"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="103" mass="11767" checksum="31DA8D10B0A7835B" modified="2019-06-05" version="1">MQNQQIRIRLKAFDHRLIDQSTQEIVETAKRTGAQVRGPIPLPTRKERFTLLVSPHVNKDARDQYEIRTHKRVLDIVQPTDKTVDALMKLDLAAGVEVQISLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKX7</accession>
    <name>A0A481QKX7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="6">2.1.1.182</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">rsmA</name>
        <name evidence="6" type="synonym">ksgA</name>
        <name evidence="9" type="ORF">C3B55_00117</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>adenosine(1518)/adenosine(1519) in 16S rRNA + 4 S-adenosyl-L-methionine = 4 H(+) + N(6)-dimethyladenosine(1518)/N(6)-dimethyladenosine(1519) in 16S rRNA + 4 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:19609"/>
            <dbReference type="Rhea" id="RHEA-COMP:10232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10233"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.182"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6 7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.182" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00117"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052908">
        <property type="term" value="F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="rADc" evidence="8">
        <location>
            <begin position="23"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="268" mass="30257" checksum="45F8D772D0FD79CD" modified="2019-06-05" version="1">MIEYYQHRARKRFGQNFLQDASVIKQIMHSINATPEDRLLEIGPGKGALTQSLLDSGAQLDAVELDKDLIPILNQRFANKLNFNLHQGNALKFNLDSLNTAPNRLRVVGNLPYNISTPLIFHLLNKIGTICDMHFMLQKEVVKRLAASPGSSYWGRLSIMTQYHCYVEHLFNVSPGAFNPPPQIDSAIVRLVPHAVLPHPTKHYELLKRVVREAFSQRRKTLRNTLKVLLSDAEIKAASVDSDLRPERLDLAAFVRLARQLAISNPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKY8</accession>
    <name>A0A481QKY8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sodium/proline symporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Proline permease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">putP</name>
        <name evidence="11" type="ORF">C3B55_00194</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the sodium-dependent uptake of extracellular L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00194"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005298">
        <property type="term" value="F:proline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031402">
        <property type="term" value="F:sodium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11475">
        <property type="entry name" value="SLC5sbd_PutP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011851">
        <property type="entry name" value="Na/Pro_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02121">
        <property type="entry name" value="Na_Pro_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0915">Sodium</keyword>
    <keyword evidence="7 10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0769">Symport</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="494" mass="53487" checksum="86BFE191B2DB07E0" modified="2019-06-05" version="1">MSVSNPVLITFVIYIAAMVLIGFIAYRSTNNLSDYILGGRSLGSVVTALSAGASDMSGWLLMGLPGAIYMFGLSESWIAIGLIVGAYLNWFFVAGRLRVQTEHNGDALTLPDYFSSRFEDKSGLLRIISAVVILVFFTIYCASGIVAGARLFESTFGMSYALALWAGAAATIAYTFVGGFLAVSWTDTVQATLMIFALILTPIIVLLATGGIDTTFLAIEAKNPDNFNMIKNTTFIGIISLMGWGLGYFGQPHILARFMAADSVKSIAKARRISMIWMILCLGGTVAVGFFGIAYFSIHPEVAYPVTENHERVFIELTKLLFNPWIAGVLLSAILAAVMSTLSCQLLVCSSAMTEDFYKTFLRKNASQVELVWVGRAMVLLISLIAIAMAANPDNRVLGLVSYAWAGFGAAFGPTVLISVIWKGMTRNGALAGILVGAITVIVWKHFNLLGLYEIIPGFIFASLAIYLVSKIGIPTNSMVERFDAAEKNYHRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL04</accession>
    <name>A0A481QL04_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Ribosomal RNA small subunit methyltransferase D</fullName>
            <ecNumber evidence="4 8">2.1.1.171</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rsmD</name>
        <name evidence="9" type="ORF">C3B55_00078</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Specifically methylates the guanine in position 966 of 16S rRNA in the assembled 30S particle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>guanosine(966) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(2)-methylguanosine(966) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:23548"/>
            <dbReference type="Rhea" id="RHEA-COMP:10211"/>
            <dbReference type="Rhea" id="RHEA-COMP:10212"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74481"/>
            <dbReference type="EC" id="2.1.1.171"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the methyltransferase superfamily. RsmD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.171" evidence="4 8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00078"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052913">
        <property type="term" value="F:16S rRNA (guanine(966)-N(2))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004398">
        <property type="entry name" value="RNA_MeTrfase_RsmD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004553">
        <property type="entry name" value="CHP00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00095">
        <property type="entry name" value="TIGR00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 8 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR004553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="205" mass="23281" checksum="55778D761D64BF31" modified="2019-06-05" version="1">MTSLFRQKKFIHNIHRSMGQLRIIGGEWSNFKLSLPDVVDLRPTLNNVRETLFNWLAPYISGAKVLDPFTGSGALFLEALSRGAAHGLSLDTSNIAVSKLKEHLRTLHCTTGEVQTVDALRYLEIQATNRYDIVFLDPPFNQNLLPTVCALLEKRQWLAVDAWIYTESGSSLSTVSLPSSWRLHREHKSGRVYYALWHRFVEGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL33</accession>
    <name>A0A481QL33_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00253"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016936">
        <property type="entry name" value="UCP029693"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10095">
        <property type="entry name" value="DUF2333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029693">
        <property type="entry name" value="UCP029693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="349" mass="39039" checksum="4E814B4E9F8259D6" modified="2019-06-05" version="1">MLDWKNHAGMVKNPATHSKSIHLLISKVFLGVLVVYLLIISSLGWYWSKEPALFPVKKDVEIVVEREKKQVVVGCITVDTLKTVVSTLLNKPGGFISNDRFPPGLWMDNMPSWEYGVLVQVRDLTRALRKDFARSQSQSAEDTDLANAEPRFNFDNKSWVLPASESEYQDGINFLSRYEARLSDPHQKNALLYARADNLNNWLGDVATRLGSLSQRLSASVGQVQLNTALKPEVIASGKVSQVDDEVVETPWMQIDNIFYEARGQAWALYHLLSAIEVDFIDVLAKKNATVSVRQIIRELEASQEPVWSPMILSGSGFGVLANHSLVMANYISRANAAVIDLRQLLKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL42</accession>
    <name>A0A481QL42_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L7/L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">rplL</name>
        <name evidence="7" type="ORF">C3B55_00269</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Part of the ribosomal stalk of the 50S ribosomal subunit. Forms a multimeric L10(L12)X complex, where L10 forms an elongated spine to which 2 to 4 L12 dimers bind in a sequential fashion. Binds GTP-bound translation factors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00269"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_L7/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_L7/L12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_L7/L12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_L7/L12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="PTHR45987"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="SSF48300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00855">
        <property type="entry name" value="L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L12_N" evidence="6">
        <location>
            <begin position="5"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L12" evidence="5">
        <location>
            <begin position="55"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="121" mass="12670" checksum="70428AC5B041571F" modified="2019-06-05" version="1">MSISQEDILNAVAKMSALQVVELIKAFEEKFGVSAATAAVNSSIAVTPAEEQTEFSVMLLEAGEKKISVIKAVRELTGLGLKEAKAVVDGAPARVLEAVSKDSADKAKLVLEEAGAKVELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL57</accession>
    <name>A0A481QL57_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Triosephosphate isomerase</fullName>
            <shortName evidence="3">TIM</shortName>
            <shortName evidence="3">TPI</shortName>
            <ecNumber evidence="3 4">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">tpiA</name>
        <name evidence="5" type="ORF">C3B55_00368</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="3 4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00368"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3 4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="3 4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="233"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="3">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="251" mass="26412" checksum="27A1A658B2286BD4" modified="2019-06-05" version="1">MRRTMVAGNWKMHGTRSSIAELINSLRCLALPSSVDIAVFPPCLYIKQVVDGLKGNLIQVGAQNSAVEPTQGALTGEISPSQLADAGCSYVIVGHSERRRMMGEHDGIVNRKFAAAQACGLIPVLCIGEALEQRESGNTLKVVSYQLGSLIKELGMSVFANAVIAYEPIWGIGTGLTATPQQAQDVHAAIRTQLSEETSEIAQGIRLLYGGSVKAANAAELFSMPDIDGGLIGGASLNANEFGEICRAAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL63</accession>
    <name>A0A481QL63_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00142"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="38" mass="4225" checksum="E4F177F082F0054E" modified="2019-06-05" version="1">MSYDYVFIAEVYPLAASMAMLILLATTNYNVVFVGLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL73</accession>
    <name>A0A481QL73_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">K(+)/H(+) antiporter NhaP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Potassium/proton antiporter NhaP2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">nhaP2</name>
        <name evidence="9" type="ORF">C3B55_00191</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">K(+)/H(+) antiporter that extrudes potassium in exchange for external protons and maintains the internal concentration of potassium under toxic levels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>H(+)(out) + K(+)(in) = H(+)(in) + K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. NhaP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00191"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01075">
        <property type="entry name" value="NhaP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030151">
        <property type="entry name" value="NhaP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023729">
        <property type="entry name" value="NhaP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507:SF7">
        <property type="entry name" value="PTHR32507:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="2 7" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="8">
        <location>
            <begin position="402"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="576" mass="61264" checksum="49459C23C53C5AA7" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL75</accession>
    <name>A0A481QL75_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00134"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019960">
        <property type="entry name" value="T1SS_VCA0849"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03661">
        <property type="entry name" value="T1SS_VCA0849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="1171" mass="123831" checksum="896A696AC23E9ED0" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL78</accession>
    <name>A0A481QL78_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpsH</name>
        <name evidence="6" type="ORF">C3B55_00291</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit. Contacts proteins S5 and S12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00291"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_B">
        <property type="entry name" value="Ribosomal_S8_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="130" mass="14110" checksum="6253C148E3ADFB2E" modified="2019-06-05" version="1">MSMQDPLADMLTRIRNAQMAAKFTVSMPSSKLKVAVAKVLKDEGYIAEYHISSEIKPVLSIELKYFEGRAVIEELKRVSRPGLRQYKSAKNLPKVCGGLGVSIVSTNKSVMTDRAACAAGIGGEVLCTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL80</accession>
    <name>A0A481QL80_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00309"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="41" mass="4583" checksum="1D77D3C0F361E3B0" modified="2019-06-05" version="1">MAFLSDFSPEPKTHELLMVDNQHSNPTSAENVVRVISEALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL88</accession>
    <name>A0A481QL88_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00174"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="45" mass="5419" checksum="CC1F4465DD26D797" modified="2019-06-05" version="1">MNIFWLYMQIDILLIVPACNTKKPKNLEFDIKQSISSHHKIYKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLC1</accession>
    <name>A0A481QLC1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sulfurtransferase DsrE</fullName>
            <ecNumber evidence="5">2.8.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dsrE</name>
        <name evidence="5" type="ORF">C3B55_00462</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DsrE/TusD family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00462"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017463">
        <property type="entry name" value="Sulphur_relay_TusD/DsrE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03012">
        <property type="entry name" value="sulf_tusD_dsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="130" mass="14680" checksum="C443F1BF813A24BD" modified="2019-06-05" version="1">MKFVIAVFSAAHMPSSRRALLFAQALLANRHEIIRLFFYQDGAYNASNNIVVPKNEQDTALQWREFVSAHQLDGVVCITAALRRGMLNTEEATRYQRRAVNLDTTWILSGLGQLHDAVQTADRLICFRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLH8</accession>
    <name>A0A481QLH8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Transcription termination/antitermination protein NusG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nusG</name>
        <name evidence="9" type="ORF">C3B55_00265</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Participates in transcription elongation, termination and antitermination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the NusG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00265"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00948">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
        <property type="entry name" value="NusG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001062">
        <property type="entry name" value="Transcrpt_antiterm_NusG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015869">
        <property type="entry name" value="Transcrpt_antiterm_NusG_bac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
        <property type="entry name" value="PTHR30265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265:SF2">
        <property type="entry name" value="PTHR30265:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02357">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00338">
        <property type="entry name" value="NUSGTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="SSF82679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00922">
        <property type="entry name" value="nusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01014">
        <property type="entry name" value="NUSG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="3 5 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="NGN" evidence="7">
        <location>
            <begin position="2"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="8">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="177" mass="20155" checksum="C0C7D60BC60A6D1B" modified="2019-06-05" version="1">MAKRWYVVHAYSGYEKHVMRSLLERVKLSCMEDSFGDILVPTEEVVEMRNGQKCKSERKFFPGYVLVQMNMNESAWHLVRDTPRVMSFIGGAADKPAPITDKEAEAILRRVADGSDKPKPKTLFEPGEIVRVTDGPFSDFNGTVEEVNYEKNRIQVAVLIFGRPTPVELEFSQVEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLI0</accession>
    <name>A0A481QLI0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="6">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">coaD</name>
        <name evidence="8" type="ORF">C3B55_00077</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the bacterial CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="reaction ID" value="UER00355"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001980">
        <property type="entry name" value="PPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF1">
        <property type="entry name" value="PTHR21342:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01020">
        <property type="entry name" value="LPSBIOSNTHSS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01510">
        <property type="entry name" value="coaD_prev_kdtB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="3 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="123"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029346"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="159" mass="17911" checksum="5AA64DD17DC7C8B1" modified="2019-06-05" version="1">MNRVLYPGTFDPITKGHGDIVERASLLFDHMIIAVAASPKKNPLFPLEQRVELVREVTKHLPNVEVAGFSTLLAHFAREQNVNVFLRGLRAASDFEYEFQLANINRQLAPDVESLFFMPAERYSFISSTLVREIAALGGDITKFVHQDVADALSIHFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLJ8</accession>
    <name>A0A481QLJ8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpmC</name>
        <name evidence="6" type="ORF">C3B55_00285</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
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            <taxon>Gammaproteobacteria</taxon>
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            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
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    </comment>
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        <property type="protein sequence ID" value="QAX81640.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00021211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="63" mass="7332" checksum="A50368C20DC56994" modified="2019-06-05" version="1">MKTNELRKKSVQQLNEQLLSLLRDQFNLRIQKATGQLRQSHLLSQVKCDIARLKTVLNQQTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLK3</accession>
    <name>A0A481QLK3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">50S ribosomal protein L30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rpmD</name>
        <name evidence="7" type="ORF">C3B55_00295</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
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            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
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        <property type="protein sequence ID" value="QAX81650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005996">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00327">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002211">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="SSF55129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01308">
        <property type="entry name" value="rpmD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L30" evidence="6">
        <location>
            <begin position="4"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="58" mass="6735" checksum="D05A3B91DF2D4D51" modified="2019-06-05" version="1">MNTIKVTLIKSMTGRIPNHRLCVKGLGLRRIHHTVEVQDSPEIRGMINKVHYMLRVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLL5</accession>
    <name>A0A481QLL5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0434 protein C3B55_00466</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0434 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00466"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01187">
        <property type="entry name" value="UPF0434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="61" mass="6759" checksum="469732E3F6F9C9BE" modified="2019-06-05" version="1">MDTKLLDILACPICKNLLKLSADKTELISKDAGLAYPIRDGIPVMLESEARTLTVDERLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLL9</accession>
    <name>A0A481QLL9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Single-stranded DNA-binding protein</fullName>
            <shortName evidence="2">SSB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ssb</name>
        <name evidence="6" type="ORF">C3B55_00308</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00308"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00984">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="PTHR10302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="112"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Important for interaction with partner proteins" evidence="2">
        <location>
            <begin position="163"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="112"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="168" mass="18804" checksum="168776AC33A2E42F" modified="2019-06-05" version="1">MARGVNKVILIGTCGQDPEVRYLPNGNAVINLSLATNEQWIDKQTSQKIEKTEWHRVAMFGKVAEIAGEYLRKGSQVYIEGKLQTREWEKDGIKRYTTEIVVDMQGTMQLLGSRSQGDQHGHGSNVSASVPRHQQAQRESRLTPTPTLQPVTSQTATSFDSFDDDIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLQ3</accession>
    <name>A0A481QLQ3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00589</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00589"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="61" mass="6961" checksum="0906112BBA88FB5E" modified="2019-06-05" version="1">MRLFMLVLLAIKLSWLIQQIIKFAWQITLESQVVSHTLCKTLGEVYAKLATEDAVSVIKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLQ6</accession>
    <name>A0A481QLQ6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphoheptose isomerase</fullName>
            <ecNumber evidence="10">5.3.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Sedoheptulose 7-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">gmhA</name>
        <name evidence="12" type="ORF">C3B55_00588</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>2 D-sedoheptulose 7-phosphate = D-glycero-alpha-D-manno-heptose 7-phosphate + D-glycero-beta-D-manno-heptose 7-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27489"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:60203"/>
            <dbReference type="ChEBI" id="CHEBI:60204"/>
            <dbReference type="EC" id="5.3.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the SIS family. GmhA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.28" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00588"/>
    <dbReference type="UniPathway" id="UPA00041">
        <property type="reaction ID" value="UER00436"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008968">
        <property type="term" value="F:D-sedoheptulose 7-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001061">
        <property type="term" value="P:D-glycero-D-manno-heptose 7-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05006">
        <property type="entry name" value="SIS_GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00067">
        <property type="entry name" value="GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035461">
        <property type="entry name" value="GmhA/DiaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004515">
        <property type="entry name" value="Phosphoheptose_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13580">
        <property type="entry name" value="SIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SIS" evidence="11">
        <location>
            <begin position="36"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="93"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="119"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="197" mass="21309" checksum="69300670EBB4CE9E" modified="2019-06-05" version="1">MDMKSRICQIFQASISTKQQAMDILAPHIEQAGQIIVKAFLSDGKMLSCGNGGSASDAQHISSELLNRFERERPSLPAIALTTDSSTITSIANDYSYNEIFSKQIYALGHPGDVLLAISTSGNSENVIQAIQAAHDRKMIIVALTGCGGGMMASSLFKEDVEIRVPANVIARIQEVHLLTIHCLCDLIDNQLFGSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLR5</accession>
    <name>A0A481QLR5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Endoribonuclease YbeY</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">ybeY</name>
        <name evidence="10" type="ORF">C3B55_00338</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00338"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020549">
        <property type="entry name" value="YbeY_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986">
        <property type="entry name" value="PTHR46986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00043">
        <property type="entry name" value="TIGR00043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01306">
        <property type="entry name" value="UPF0054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 9" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="152" mass="17366" checksum="F5365E94BD40FD2B" modified="2019-06-05" version="1">MLELDLQLATNTPAPNKEQFHKWCSIALRQRIASSELTIRLVDELEGRALNQTWRQKNYATNVLSFPAEVPAELLDIPLLGDLVICIEVVIREAKAQDKELEAHWAHIVIHGCLHLLGYDHINDDEAREMEALEQKLLAELDYPDPYTDDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLS6</accession>
    <name>A0A481QLS6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proline-specific permease ProY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">proY</name>
        <name evidence="7" type="ORF">C3B55_00553</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00553"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="6">
        <location>
            <begin position="26"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="468" mass="51169" checksum="D341368F33CEAF21" modified="2019-06-05" version="1">MLAGNPLLHGETAQGSLLKRELSERHIRLMALGACIGAGLFFGSAKAIALAGPSIMLSCIVGGLAILVIMRALGEMAVYNPVAGSFSRYAQDYLGPLAGFLVGWNYWFLWLVTCMAEITAIAVYMGIWFQNVPRWIWAMAALASMSTINLIAVKTFGEFEFWFALIKIFTIIAMMIGGISIIAFGFGNNGVALGITNLWVHGGFMPNGVTGVLMSLQMVMFAYLGVEMIGLTAGEAKNPQRIIPNAIGSVFWRILLFYVGALFVILSIYPWNEISTQSSPFVMTFERLGIKTAAGIINFVVITAALSSCSGGIFSTGRMLYSLAQNGQAPATFGTTSSNGVPRKALLLSIFMLLLGVLLNYMVPEKVFLWVTSIATFSAIWTWLMILLSQLKFRKSLNAVEVDCLKYRMWLYPISSYMALAFLILVLGLMAYFPDTRVALYVGPVFLVLLMVLFYAFKLKPTNASVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLV9</accession>
    <name>A0A481QLV9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Elongation factor G</fullName>
            <shortName evidence="8">EF-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">fusA</name>
        <name evidence="10" type="ORF">C3B55_00274</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00274"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="8"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="89"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="143"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="701" mass="77322" checksum="7C8B41989BD3C759" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLX5</accession>
    <name>A0A481QLX5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00594"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="149" mass="16562" checksum="9926D6F0CCAE1FE9" modified="2019-06-05" version="1">MHALFVLGDLRAKLVKQFQSSFVYIAEQTPEGMYIAEIHTGSAMVVDDKPRLELKVGDHFCAAILPSREGGKLELKFRDIKLTVYGLGDYAFVSSADGQGIIFKEGHSLMLVFATNEQRQEGLIKILKAVISKVAKWRNGELVTFKASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLY0</accession>
    <name>A0A481QLY0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="6">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">hisC</name>
        <name evidence="8" type="ORF">C3B55_00511</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00511"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="7">
        <location>
            <begin position="24"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="350" mass="38924" checksum="7018E5AF50E7D3EA" modified="2019-06-05" version="1">MSKFWSQFVYDLVPYVPGEQPKLTKLIKLNTNENPYGPSPKALAAMHAELSDNLRLYPDPNGDLLKQAVAKHYGIDASKIFLGNGSDEVLAHIFHSLFQHDLPLLFPDISYSFYPVYCGLYGIKFTLVPLDTQFQIRVADYTTPNGGIIFPNPNSPTGCVLALDAVEQILKASPDSVVVVDEAYIDFGGKTAISLADCYPNLLVTQTLSKSRSLAGLRVGLAIGHPDLIRALELVKNSFNSYPLDSLAIAGAAASFKDREYFEKTCQLVIKSRNKMVTQLEGKGFEVLPTAANFIFARHLKYDAAWLVTKLREREVIVRHFKQERIAQFLRISIGTPEQNQALIDALEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLY7</accession>
    <name>A0A481QLY7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfurtransferase TusA</fullName>
            <ecNumber evidence="3">2.8.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tusA</name>
        <name evidence="3" type="ORF">C3B55_00720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00720"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="81" mass="9297" checksum="2FAB7C49BBE837A5" modified="2019-06-05" version="1">MNTDLSVNSILDATGLHCPEPLMMLHQHIYVLPVGCLLKVIATDPSTCRDIPKFCMFLDYELVEQQEQAGTYLYWIRKKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLZ0</accession>
    <name>A0A481QLZ0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative protease SohB</fullName>
            <ecNumber evidence="9">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sohB</name>
        <name evidence="9" type="ORF">C3B55_00471</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00471"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013703">
        <property type="entry name" value="Peptidase_S49_N_proteobac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08496">
        <property type="entry name" value="Peptidase_S49_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S49_N" evidence="8">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S49" evidence="7">
        <location>
            <begin position="153"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="341" mass="38483" checksum="5B0CBADD04BDAF44" modified="2019-06-05" version="1">MDFLAEYARFLAKTFTIVIAILVILISCVTLRSKGRRKSIGQLQINKLNDFYKGLRESLELSLLDKNQLQALRKSERKSEKKKCKKKIEAKQRVFVLNFDGDIKASATNNLRHEITALLSMATPKDEVVLLLESGGGMVHNYGLAASQLARLHQAGVPLTVCIDKVAASGGYMMACIGKKIISAPFAILGSIGVITQLPNINRLLKKHDIDFEVLTAGEYKRTITVFGENTEKDREKLQQDLNITHQLFKNFVARYRPQLAIDEVATGEVWLGTAALDKQLVDKLQTSDEYLSIKAETAEVFHLHYAEHKSLKERIGLAASSAVDRVLLTWWRRLTQRRFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLZ8</accession>
    <name>A0A481QLZ8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cell shape-determining protein MreB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mreB_2</name>
        <name evidence="2" type="synonym">mreB</name>
        <name evidence="3" type="ORF">C3B55_00533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms polymers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Membrane-associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the FtsA/MreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00533"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10225">
        <property type="entry name" value="MreB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02207">
        <property type="entry name" value="MreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004753">
        <property type="entry name" value="MreB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01652">
        <property type="entry name" value="SHAPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00904">
        <property type="entry name" value="mreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="214"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="294"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="345" mass="37126" checksum="33151FEF788929EA" modified="2019-06-05" version="1">MFKKLRGMFSSDLSIDLGTANTLIYVRERGIVLNEPSVVAIRTHGNQKSVVAVGTEAKRMLGRTPSNIAAIRPMKDGVIADFSVCEKMLQYFIHKAHENSFLQPSPRVLICVPCKSTQVERRAIRESALGAGAREVFLIEEPMAAAIGAGLPVEEAHGSMVVDIGGGTTEIALISLNGVVYAESVRVGGDRFDEAIITYVRRNYGNLIGESTAERIKQEIGAAYQGSEVREVDVRGRNLAEGVPRAFTLNSNEALEALQESLATIVQAVRSVLEQSPPELASDIAERGLVLTGGGALLRDLDKLLAQETSLPVIVAEDPLTCVARGGGRALEMMDKHTMDLLSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLZ9</accession>
    <name>A0A481QLZ9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rod shape-determining protein MreD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mreD_2</name>
        <name evidence="10" type="ORF">C3B55_00531</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in formation of the rod shape of the cell. May also contribute to regulation of formation of penicillin-binding proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MreD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00531"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007227">
        <property type="entry name" value="Cell_shape_determining_MreD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026034">
        <property type="entry name" value="MreD_proteobac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37484">
        <property type="entry name" value="PTHR37484"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04093">
        <property type="entry name" value="MreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018472">
        <property type="entry name" value="MreD_proteobac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03426">
        <property type="entry name" value="shape_MreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="162" mass="18581" checksum="EF13F698A7848CAF" modified="2019-06-05" version="1">MAYTNSRNSCIVWLNFVIGLLLSVSPLPQFMEILRPLWLALLLAFWSLIMPHTVGLITAMCLGLAEDILYGTLLGQNALLLTIAIFLMQSLQQRLRMFFMWQQCLMILVIFGLMQLVQFWLSALTGNRQPTLALMLPALISALLWPCISFSLRGLRRRYKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM08</accession>
    <name>A0A481QM08_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00344"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="219" mass="24610" checksum="E82F16E272D47D9E" modified="2019-06-05" version="1">MNLMNPLDILRDSFRFTQLNLGAIIQLCLPLVIPEALLQQVLNHILGQDAFPGYNVVAGLLVYPLYTGALIMFLDARTHGKSPNTKDIWTIALNLWPRFALLTAISTLLILLGLSLYVLPGLCLMVILAFAEYLLVLRSMSVLEAMKESVRLTHRRFWLILVCLLCVMTPLWLLKGASEVTYPNPTLLLGVLIDSTYSFLQLFASVVLFRLFMLIDDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM17</accession>
    <name>A0A481QM17_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Outer membrane protein assembly factor BamA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">bamA</name>
        <name evidence="9" type="ORF">C3B55_00763</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BamA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00763"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01430">
        <property type="entry name" value="OM_assembly_BamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010827">
        <property type="entry name" value="BamA/TamA_POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023707">
        <property type="entry name" value="OM_assembly_BamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="PTHR12815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF23">
        <property type="entry name" value="PTHR12815:SF23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07244">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006076">
        <property type="entry name" value="OM_assembly_OMP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03303">
        <property type="entry name" value="OM_YaeT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 6" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="POTRA" evidence="8">
        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="8">
        <location>
            <begin position="100"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="8">
        <location>
            <begin position="183"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="8">
        <location>
            <begin position="355"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="670"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="804" mass="89755" checksum="B37D230348398479" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM30</accession>
    <name>A0A481QM30_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">ftsZ</name>
        <name evidence="8" type="ORF">C3B55_00572</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00572"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 5 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="6">
        <location>
            <begin position="13"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="7">
        <location>
            <begin position="207"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="21"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="108"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="401" mass="42134" checksum="99B193FAB7F2A5D6" modified="2019-06-05" version="1">MFELVDNIPASPVIKVIGVGGGGGNAVNHMVKSDIEGVEFICANTDAQALKSISAQTILQLGTAVTKGLGAGANSEVGRQAALEDRERIAAVLQGTNMVFITTGMGGGTGTGAAPIIAEVAKEIGILTVAIVTRPFSFEGRKRMQIADEGIRLLSENVDSLITIPNEKLLTILGKDVSLLSAFAKADDVLAGAVRGISDIIKRPGMINVDFADVRTVMSQMGMAMMGTGCASGPNRAREATEAAIRNPLLEDVNLQGAHGILVNITSGPDLSLGEYSDVGSIIDAFSSEHAMVKVGTVIDPDMHDELHVTVVATGLGTKIDKPMQAIANTIHTSHSSNICQAATAHAPAHHELPPVNYRDLDHPTVMRNQAQTGVAAFRSMNSQDDLDYLDIPAFLRRQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM44</accession>
    <name>A0A481QM44_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Endonuclease III</fullName>
            <ecNumber evidence="9">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">nth</name>
        <name evidence="12" type="ORF">C3B55_00783</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00783"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01083">
        <property type="entry name" value="nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="11">
        <location>
            <begin position="38"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="10">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="211" mass="24015" checksum="092725342EB46F09" modified="2019-06-05" version="1">MNTEKRLEIFRKFHEDNPEPKTALIYSSPFELLISVILSAQSTDVSVNKATAKLYQVANTPETVYALGIDGLSEHIKTINLYKNKTKNIIKTCRMLVNLHGNKVPQNREDLEALPGVGRKTANVILNTAFQQLTIAVDTHIFRVSNKTGITRGKNVFEVEMQLMKFVPKPYLLNSHHWLVLHGQYICRARKPLCSNCSIKDLCEFKYKNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM46</accession>
    <name>A0A481QM46_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptide chain release factor 3</fullName>
            <shortName evidence="6">RF-3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">prfC</name>
        <name evidence="8" type="ORF">C3B55_00546</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. PrfC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00546"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04169">
        <property type="entry name" value="RF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00072">
        <property type="entry name" value="Rel_fac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004548">
        <property type="entry name" value="PrfC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032090">
        <property type="entry name" value="RF3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038467">
        <property type="entry name" value="RF3_dom_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041732">
        <property type="entry name" value="RF3_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43556">
        <property type="entry name" value="PTHR43556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16658">
        <property type="entry name" value="RF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00503">
        <property type="entry name" value="prfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="9"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="18"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="528" mass="59601" checksum="AE41BF96750AB48D" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM51</accession>
    <name>A0A481QM51_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6 7" type="primary">rplM</name>
        <name evidence="7" type="ORF">C3B55_00539</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 6">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00539"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_L13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_L13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="142" mass="15786" checksum="C0430CE142D06518" modified="2019-06-05" version="1">MITFTAKPKTVQRDWFVVNAAGQTLGRLATKVAIHLRGKHKPEYTPHIDTGDYIIVINAKQIRLTGNKAKHKMYYRHSGFPGGIKSSNFEGLIAKKPEAPIEIAVKGMLPRGPLGRDMLRKLRVYAGTVHPHAAQQPQALKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM54</accession>
    <name>A0A481QM54_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH-type transcriptional regulator CysB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cysB</name>
        <name evidence="3" type="ORF">C3B55_00682</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00682"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08413">
        <property type="entry name" value="PBP2_CysB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037423">
        <property type="entry name" value="CysB_PBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="324" mass="36272" checksum="215BE40BFBF5A965" modified="2019-06-05" version="1">MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGIEVFARSGKHLTRVTPAGERITITAGEILRKVESIKQIAQEFSNEKKGTLSIATTHTQALYALPSVIRDFIKQYPDVALHMHQGSPMQISEMAANGAVDFAISTEALELFGDLVMMPCYRWNRCVIVPQTHPLAKLLKLTIETLAEYPILTYVFGFAGRSKLDEAFSHHSLTPKVVFTAADADVIKTYVRLGLGVGIVAKMAVDANLDKDLVVLDASELFESSVTKIGFRRGTFMRGFMCDFIEKFAPHLTREVMAKAMQCNNKQELEKLFDMVELPVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM68</accession>
    <name>A0A481QM68_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aspartate aminotransferase</fullName>
            <ecNumber evidence="5">2.6.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">aspC</name>
        <name evidence="5" type="ORF">C3B55_00831</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00831"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="PTHR11879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="4">
        <location>
            <begin position="28"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="400" mass="44076" checksum="FB8F0F92EC67B6D1" modified="2019-06-05" version="1">MHFNAIGRVSSDPILGLMSSYSKDNNPNKFDLGVGVYKNNQGFTPILHSVKLAEQRLVETQITKTYISSHGNRTFGALISQLVLNTNSALIHDRRVSVTQTPGGTGALRLSADFINHNLKGCNVWLSNPTWPIHETIFAKAGLTVNHYQYVSTHNRLDITAMLATLATIPKGDVVLLQACCHNPTGFDLSQNNWRQVLQIVRKRQLLPLIDFAYQGFGDGVEQDAWAVRLFAEELPEVLITSSCSKNFSLYSDRVGALIVCSADAKKLSNVRSQLANIACNLWATPPDHGASVVATILADTKLKQVWSNEVEAMRLRIIQLRSELVKALALHGLVEQFAHIDTQRGMFSYTGLSAEQVKQLRKKYSVYMVSSGRVNIAGIDATRLTILAQAIADVSNCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM69</accession>
    <name>A0A481QM69_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fe(3+)-citrate-binding protein YfmC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yfmC</name>
        <name evidence="3" type="ORF">C3B55_00616</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00616"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019741748" evidence="1">
        <location>
            <begin position="25"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="44"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="306" mass="33131" checksum="5BA64BFC7AFBB155" modified="2019-06-05" version="1">MQMLHYISTLAACILACSSNVLNAAPIDLNDGQHTIHLVGAPKRVIVLEFSFLDSLAAIDVMPIGIADDGAANRVLPSAYKAIGQWESVGLRSQPNIEKIARLKPDLILADLNRHQALYDHLSSIAPTLLLPSRSEDYQGSLKSAKLIGKALGKSLQMDARIAQNCEHLKAIAQQIPAGTSVLFGVAREDSFSVHGQDSYAGSVLKTIGLKVSSVSAKAVPTEFISLEQLLALDPGWLLVSHYRRPSIIDTWSKLPLWQVLSAVRNHQVAEVNGDNWARHRGVLASEQIAEDALVILKNGKAVLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM76</accession>
    <name>A0A481QM76_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase I</fullName>
            <ecNumber evidence="6">2.1.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA 2'-O-ribose C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">rRNA (cytidine-2'-O-)-methyltransferase RsmI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">rsmI</name>
        <name evidence="8" type="ORF">C3B55_00585</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(1402) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42924"/>
            <dbReference type="Rhea" id="RHEA-COMP:10285"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.198"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the methyltransferase superfamily. RsmI family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.198" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00585"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070677">
        <property type="term" value="F:rRNA (cytosine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000453">
        <property type="term" value="P:enzyme-directed rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11648">
        <property type="entry name" value="RsmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01877">
        <property type="entry name" value="16SrRNA_methyltr_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008189">
        <property type="entry name" value="rRNA_ssu_MeTfrase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46111">
        <property type="entry name" value="PTHR46111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005917">
        <property type="entry name" value="MTase_YraL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00096">
        <property type="entry name" value="TIGR00096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="292" mass="32259" checksum="FCFDF33A85A6A408" modified="2019-06-05" version="1">MRFDCARNLLNSTAGSLFVVATPIGNLDDISTRALKVLREVKLIAAEDTRHSLRLMQHFGVSTPLASCHKHNERDASSRFIARLLTGDDIALIADAGTPLIADPGYHLVYQARAASINVVPIPGVCALIAALSAAGLSSDRFLFDGFLPAKTFARRARLRTLKEEPRTLIFYEAPHRILECLQDMELVFGGKRLALLARELTKTFETIKGLPLEELHAFVQDDTNQQRGECVVLVAGWTKLQNHNEVGSEAIRILDLLLKEMPLKCAVLLAAKITGLRRNALYQVALDKQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM80</accession>
    <name>A0A481QM80_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00626</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00626"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="52" mass="6037" checksum="7C15A6AF3DF817BB" modified="2019-06-05" version="1">MVVAYNQKTSRTQTLDANGLRQNTSLQCFIKIFASREQTKANNKIYERKSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM91</accession>
    <name>A0A481QM91_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="7">SHMT</shortName>
            <shortName evidence="7">Serine methylase</shortName>
            <ecNumber evidence="7">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">glyA2</name>
        <name evidence="7" type="synonym">glyA</name>
        <name evidence="10" type="ORF">C3B55_00349</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00349"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SHMT" evidence="9">
        <location>
            <begin position="9"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="354"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7 8">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="417" mass="45251" checksum="5CEA930F80700505" modified="2019-06-05" version="1">MFSRNLTIAEYDADLCTAMKQEATRQEEHIELIASENYTSPAVMEAQGSTLTNKYAEGYPGKRYYGGCEYIDAVEQLAINRAKKLFDADYVNVQPHAGSQANGSVYLALLQGGDTILGMSLAHGGHLTHGASVSSSGKLYNAIQYGIDANGLIDYNAVEYLAVKHKPKIIVAGFSAYSQILDFQHFRTIADKVGAYLFVDMAHVAGLVAAGVYPNPVPYADVVTTTTHKTLRGPRGGLILARANTEIEKKLNSAVFPGTQGGPMEHVIAAKAVCFKEALQPEFKTYQQQVVKNAKTMAKVFIERGFEVVSGGTKNHLFLLSLIKQNISGKDADAALGKAFITANKNSVPNDPRSPFITSGLRFGTPAITTRCFKEAECTELAAWICDILSDLHNESVIDEVREKVKVICKKLPVYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMA0</accession>
    <name>A0A481QMA0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="9">HTPA reductase</shortName>
            <ecNumber evidence="9">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">dapB</name>
        <name evidence="12" type="ORF">C3B55_00360</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00360"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DapB_N" evidence="10">
        <location>
            <begin position="3"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="DapB_C" evidence="11">
        <location>
            <begin position="129"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="(S)-2,3,4,5-tetrahydrodipicolinate binding" evidence="9">
        <location>
            <begin position="166"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="99"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="123"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="268" mass="28950" checksum="E3440CEA29E018B5" modified="2019-06-05" version="1">MRRIAVIGAAGRMGKKLIKEVHQRSLVSRLTAAIIRPDSTLIGMDAGELVSLGRIGILLSGSLEHVANEFDVLIDFTLPDVMIKNLIFCRKAGKAMVIGTTGLSQDQKHLLVEASTDIPIVFAANFSVGVNLSLKLLDLAAHVLGDEADIEIIETHHRHKLDMPSGTALRMGETIADALGRDLSKVAVYGHESRTGTRARETIGFAVVRGGDVIGDHTVLFATEGERLEITHKASSRMTFASGAVRAALWLESRKPGLYDMSNVLDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMA1</accession>
    <name>A0A481QMA1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase D</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ppiD</name>
        <name evidence="4" type="ORF">C3B55_00601</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00601"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="3">
        <location>
            <begin position="263"/>
            <end position="358"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="QAX81933.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="620" mass="69978" checksum="B29E572EE8E9029A" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="12" xmlns="http://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="8">Serine transporter</fullName>
        </submittedName>
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        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
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            <authorList>
                <person name="Weglarz K.M."/>
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            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
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        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="429" mass="46990" checksum="0AAE51F37F59C0B6" modified="2019-06-05" version="1">MNHQANGVEERYITTSVTLTSWSRQDTTWMLGLFGTAIGAGTLFLPINAGLGGFWPLVILSLLAFPMTFFAHRSLTRFILSSHDGSDITGVVEEHFGLKAGMLITLLYFFAIFPILLIYSVALTNTVSSFMEHQLHIMPPPRVVMAFALILSLLAVVLCGEQVIVKVMSLMVYPFIVALLLLGVYLIPHWTGGIFSTISYVPEPSTLLNTFWLAIPVMVFSFNHSPIISAFAVDQRRQYAANADKRSSQILYRAHLLMVSIVLFFVFSCALTLSSAQLAEAKAQNLSILSYLANQFDNPAIAFAAPLIAFMAIAKSFLGHYIGASEGLKGLIIKTGSSQAAKSLDRIIAAFILITCWFVATLNPSILGMIETLCGPIIASILFLMPMYAIRKVPAMAKYRGQVSNVFVTVVGLMAITSLIYSFISIIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMC5</accession>
    <name>A0A481QMC5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acyl-coenzyme A dehydrogenase</fullName>
            <ecNumber evidence="6">1.3.8.7</ecNumber>
            <ecNumber evidence="7">1.3.8.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">fadE</name>
        <name evidence="16" type="ORF">C3B55_00645</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a long-chain 2,3-saturated fatty acyl-CoA + H(+) + oxidized [electron-transfer flavoprotein] = a long-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:17721"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:83721"/>
            <dbReference type="ChEBI" id="CHEBI:83727"/>
            <dbReference type="EC" id="1.3.8.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a medium-chain 2,3-saturated fatty acyl-CoA + H(+) + oxidized [electron-transfer flavoprotein] = a medium-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:14477"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:83723"/>
            <dbReference type="ChEBI" id="CHEBI:83726"/>
            <dbReference type="EC" id="1.3.8.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.7" evidence="6"/>
    <dbReference type="EC" id="1.3.8.8" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00645"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004466">
        <property type="term" value="F:long-chain-acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015396">
        <property type="entry name" value="DUF1974"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09317">
        <property type="entry name" value="DUF1974"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="14">
        <location>
            <begin position="142"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="13">
        <location>
            <begin position="240"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="12">
        <location>
            <begin position="363"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1974" evidence="15">
        <location>
            <begin position="517"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00020144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF09317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX81970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="815" mass="89975" checksum="E2C3F07C0296FDAC" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMD3</accession>
    <name>A0A481QMD3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 18">Succinate dehydrogenase flavoprotein subunit</fullName>
            <ecNumber evidence="7 18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">sdhA</name>
        <name evidence="21" type="ORF">C3B55_00663</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>a quinone + succinate = a quinol + fumarate</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 18">Cell inner membrane</location>
            <topology evidence="4 18">Peripheral membrane protein</topology>
            <orientation evidence="4 18">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 18">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="7 18"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00663"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="18" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 18" id="KW-0274">FAD</keyword>
    <keyword evidence="11 18" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 18" id="KW-0813">Transport</keyword>
    <keyword evidence="10 18" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="19">
        <location>
            <begin position="11"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="20">
        <location>
            <begin position="462"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="17">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="QAX81988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="590" mass="63970" checksum="72D2907796C3CF93" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMD6</accession>
    <name>A0A481QMD6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chaperone protein HtpG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Heat shock protein HtpG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">High temperature protein G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">htpG</name>
        <name evidence="7" type="ORF">C3B55_00655</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Molecular chaperone. Has ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00655"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="PTHR11528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="SSF110942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="HATPase_c" evidence="6">
        <location>
            <begin position="30"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="A; substrate-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="C" evidence="5">
        <location>
            <begin position="560"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="634" mass="72086" checksum="1392F78CC76E1391" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMD7</accession>
    <name>A0A481QMD7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative deoxyribonuclease YcfH</fullName>
            <ecNumber evidence="2">3.1.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ycfH</name>
        <name evidence="2" type="ORF">C3B55_00872</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00872"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="264" mass="29440" checksum="92DB58DADF541AFA" modified="2019-06-05" version="1">MLVDSHCHLDLLDLVQNRDSLDAVLEAARHRGVGHFLCIGVSAKNAASVKTIAERYVDVDCSVGIHPLDLKNNKMPTFDWLLNELNHQRVVAIGETGLDYHYEPKTAKLQQVSFDMHLQAAKQTGKPVIVHTRNARADTLAMLREAALPQAGVLHCFTEDINMANAALDLGFYISMSGIVTFRNANALRNVARQIPADRLLVETDSPYLTPTPHRGKQNLPEYVRDVADYLAILRGESFERLAEQTTKNFKQIFPLAHLKPALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QME3</accession>
    <name>A0A481QME3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00778</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00778"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="49" mass="5744" checksum="275EF1C87F8F340B" modified="2019-06-05" version="1">MLFINQTTTCCLVTVLIYSVVGSKWLTYSEYFWCIASKMLKTRRMTKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMF5</accession>
    <name>A0A481QMF5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytochrome c biogenesis ATP-binding export protein CcmA</fullName>
            <ecNumber evidence="6">3.6.3.41</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ccmA</name>
        <name evidence="6" type="ORF">C3B55_00676</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.41" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00676"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015439">
        <property type="term" value="F:ABC-type heme transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005895">
        <property type="entry name" value="ABC_transptr_haem_export_CcmA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43499">
        <property type="entry name" value="PTHR43499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01189">
        <property type="entry name" value="ccmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="211" mass="23244" checksum="C990A2EDDF28B599" modified="2019-06-05" version="1">MISCLLETLALTCKRDMYLLFEHIELRLTDGDMLQISGPNGSGKTSLLRILAGLMQPTSGQVRLNGKPLNEQRNELSRNLVWIGHAAGIKDILTPEENLSWLSAMHYPASRAAILQALTDSGLKGFEDVPCHALSAGQRRRVALARLYLPGPALWILDEPFTAIDKQGVTQLEKYLAQHCEQGGLVVLTTHQALAFMPVGYRELDLGCRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMG1</accession>
    <name>A0A481QMG1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Histidine transport ATP-binding protein HisP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisP</name>
        <name evidence="8" type="ORF">C3B55_00896</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00896"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="4"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="254" mass="28539" checksum="9D6CF8B4953CE2CC" modified="2019-06-05" version="1">MYKLEIQNLYKRYGSYEVLKGVSLAAAAGDVISIIGSSGSGKSTFLRCINLLEQPHAGKILLNNEELKLFTNKDRTMKAADPKQLQRMRSRLSMVFQHFNLWSHMTALENVMEAPVYVLGMSKKDTREKAEHYLAEVGVGHRKNAFPGHMSGGEQQRVAIARALAMEPEVMLFDEPTSALDPELVGEVLKVMQDLAQEGRTMLVVTHEMGFAREVSNQLVFLHKGIVEERGNPREVLRNPQSKRLQQFLSGSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMH3</accession>
    <name>A0A481QMH3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoserine aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphohydroxythreonine aminotransferase</fullName>
            <shortName evidence="8">PSAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">serC</name>
        <name evidence="10" type="ORF">C3B55_00697</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>2-oxoglutarate + 4-(phosphooxy)-L-threonine = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>2-oxoglutarate + O-phospho-L-serine = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="8">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00697"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
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        <property type="reaction ID" value="UER00311"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00611">
        <property type="entry name" value="PSAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PTHR43247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01364">
        <property type="entry name" value="serC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6 8" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="9">
        <location>
            <begin position="6"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="8">
        <location>
            <begin position="77"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="8">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="153"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="361" mass="40358" checksum="F770EE8D2E407B79" modified="2019-06-05" version="1">MSNRAYNFCAGPAALPEVVLRRVQDEFLDWHKKGLSVIEMSHRSDEFVSIATKAEQDLRDLLYIPSNYKVLFLQGGASQQFAQLPLNLLPQDSTADYIDTGIWGQKAIKEASRYGYVNVAGTAKPYYYHAIPRQNEWKLSSNAAYVHYVANETIGGLEFDWVPEVGDVPLVCDMSSNILSRPIDVSKYGMIYASAQKNIGPSGMLVNIIREDLLGCARLLCPTTLSYKVAADNSSMYNTPPVFAWYLSGLVFEWLKEQGGVAVIGKLNKEKQCMLYDFIDASGLYSNSINLPDRSWMNVPFCLADDRLNELFLTCADERGLLNLKGHRLVGGMRASIYNAIDINAINALVSYMAEFEKEYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMH4</accession>
    <name>A0A481QMH4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00464"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="41" mass="4462" checksum="BB7B08E3857E134B" modified="2019-06-05" version="1">MLLLISANSKTQHSAVIIADVFGKLGFSYLRMIPIEAATKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMJ2</accession>
    <name>A0A481QMJ2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Efflux pump membrane transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ttgB</name>
        <name evidence="7" type="ORF">C3B55_00735</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cell inner membrane</location>
            <topology evidence="2 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000288953">
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    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004764">
        <property type="entry name" value="HAE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00915">
        <property type="entry name" value="2A0602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="470"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="880"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="904"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="930"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="975"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1007"/>
            <end position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="1051" mass="113704" checksum="AE322DECDCFA3B02" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMK1</accession>
    <name>A0A481QMK1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00586"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="59" mass="6625" checksum="E94BB30265A860E6" modified="2019-06-05" version="1">MLLTKDATKSKHKKDFLALTTAMPVEQLTVSDHETCNDQISLMPAIIDRYHFGETAGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMK4</accession>
    <name>A0A481QMK4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 13 15">tRNA (guanine-N(1)-)-methyltransferase</fullName>
            <ecNumber evidence="6 13 15">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 17" type="primary">trmD</name>
        <name evidence="17" type="ORF">C3B55_00940</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13 15">Specifically methylates guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13 15">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 13 15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13 15">Belongs to the RNA methyltransferase TrmD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="6 13 15"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00940"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N(1))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18080">
        <property type="entry name" value="TrmD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00605">
        <property type="entry name" value="TrmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002649">
        <property type="entry name" value="tRNA_m1G_MeTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023148">
        <property type="entry name" value="tRNA_m1G_MeTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016009">
        <property type="entry name" value="tRNA_MeTrfase_TRMD/TRM10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46417">
        <property type="entry name" value="PTHR46417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01746">
        <property type="entry name" value="tRNA_m1G_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000386">
        <property type="entry name" value="tRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00088">
        <property type="entry name" value="trmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 13 15" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA_m1G_MT" evidence="16">
        <location>
            <begin position="26"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="13 14">
        <location>
            <begin position="136"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX82242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="249" mass="28212" checksum="7ADD3804E1038399" modified="2019-06-05" version="1">MSNLNIEVISLFPKIFSAISEYGITGKAVRHGLLRLTYWNPQDYTSDRHHTVDDRPFGGGPGMVLQIKPLEDSLVAAKAAAKQKAKVIYLSPQGRQLTQTAVRKMANEKALIMIAGRYEGIDERFIEAYVDEEWSIGDYVLSGGELPAMVLIDAITRLLPGALGHAESAEEDSFTDGLLDCPHYTRPKIYADRHVPDVLLSGNHVRIRRWRLQQSLGRTYERRADLLEDRLLSREEKKLLEEYILVHNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QML8</accession>
    <name>A0A481QML8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 10">Uracil-DNA glycosylase</fullName>
            <shortName evidence="8">UDG</shortName>
            <ecNumber evidence="4 8 10">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">ung</name>
        <name evidence="12" type="ORF">C3B55_00732</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8 10">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 10">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="4 8 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00732"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="PTHR11264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="11">
        <location>
            <begin position="62"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="237" mass="27113" checksum="A42384562DDCDD57" modified="2019-06-05" version="1">MLQNDLTMTDNIRIKLEPSWKYALLDELNKPYMVELRAFLRREYDACKEIYPPKQLIFSALNSTPLDKVKIVILGQDPYHGPGQAHGLCFSVQPGLPIPPSLVNIYKELRRDLNINIPNHGCLQSWADQGVLMLNTTMTVERANANAHAGKGWQFFTDRIIKVVSEHQRHLVFMLWGAHAQGKQNLIDMTKHLVLTSVHPSPLSAYRGFLGCGHFSKANKFIEQNNEMPIEWRLPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMN8</accession>
    <name>A0A481QMN8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rpsB</name>
        <name evidence="7" type="ORF">C3B55_00771</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00771"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_S2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_S2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="PTHR12534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="246" mass="27563" checksum="1B11EB572D4A689F" modified="2019-06-05" version="1">MYQVNMRDMLKAGVHFGHQTRYWNPKMDQYIFGTRNKIHIINLEKTLPMLNEALVLVERLTKGKNKILFVGTKRAAGKIVVEEAMRCGSPYVDHRWLGGMLTNFKTIRASIKRLRDLEVQAEDGTFAKLTKKEALMRTRTLEKLDRSLGGIKDMGGLPDVLFVIDVDHERIAITEANKMGIPVIGVVDTNSSPTGIDYIIPGNDDAIRAIQLYMGLMADAVLRGRNYAASGTEQFIEDIPLAAQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMP0</accession>
    <name>A0A481QMP0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpmA</name>
        <name evidence="6" type="ORF">C3B55_00983</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00983"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00539">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_L27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_L27_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="PTHR15893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="85" mass="9217" checksum="E4B8FBD32CA9B485" modified="2019-06-05" version="1">MAHKKAGGSSRNGRDSEAKRLGLKMYGGQKIIPGNIIVRQRGTQFYAGYGVGMGKDHTLFAKIEGVIKFETKGALSRRYVSIVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMP5</accession>
    <name>A0A481QMP5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc metalloprotease</fullName>
            <ecNumber evidence="9">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rseP</name>
        <name evidence="11" type="ORF">C3B55_00764</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00764"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004387">
        <property type="entry name" value="Pept_M50_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42837">
        <property type="entry name" value="PTHR42837"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00054">
        <property type="entry name" value="TIGR00054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="383"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="114"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="211"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="451" mass="49816" checksum="D787A8A11A12E9B4" modified="2019-06-05" version="1">MSVLYMIVGTIVALGVLITFHEFGHFWMSRRCGVKVLRFSVGFGMPLLRWHDRRGTEFVVSAIPLGGYVKMLDGRDGEVPADQLEQSFNRKTVLQHVAIVIAGPIANFLLAMVLFWVLAVIGSQQIRPIIGAVEVDSIAAKAGLVAGQEIVSIDGEKTSSWSAVNLQLVRRFGESGTMYMVVRNYDSTIETLREVFLDHWLKSVDEPDPIKSLGVRPWRPDLLPVLAWLDQKGPAQAAGLKAGDRLLAFDGQSLSDWQQVVDLVRVLPGTRIVLKVERDGNQLNVPVTLLAHKEAQSSWVGYLGAGVKGVEWPHSMVREVRVGPLVAISESVKRTWIISVLTSESLKKMLFGELSVKNLSGPITIAKVAGNSAQSSIADFLNFLAYLSISLGILNLLPIPILDGGYLLFYLIEWVRGRPLSDRIQSWWVQIGMCLMVGVMLLALINDLERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMP8</accession>
    <name>A0A481QMP8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Daunorubicin/doxorubicin resistance ATP-binding protein DrrA</fullName>
            <ecNumber evidence="5">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">drrA</name>
        <name evidence="5" type="ORF">C3B55_00644</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00644"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="310" mass="34705" checksum="EEEFD7265FB64D63" modified="2019-06-05" version="1">MSSALFIQHLTKTYSNGFKALRGIDLNVAEGDFFALLGPNGAGKSTTIGVLSTMVKKTSGTVKIFSYNLDRSPAALKRSIGVVPQEFNFNQFEKTLDIVVTQAGYYGIPAKVAKERAEQYLTRLGLWNKRDVLSRSLSGGMKRRLMIARALVHEPRLLILDEPTAGVDIELRRSMWTFLTELNEKGITIILTTHYLEEAEHLCRNIGIIDHGVIVENTSMRNLFSQLHVETFLLNLKNKLLVTPQLLGYPSRLVDSHTLEVQVDNAMGITALFAQLTVQHIEVLSLRNKTNRLEELFVSLVEKNLVKVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMQ6</accession>
    <name>A0A481QMQ6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-oxoacyl-[acyl-carrier-protein] synthase 1</fullName>
            <ecNumber evidence="5">2.3.1.41</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fabB</name>
        <name evidence="5" type="ORF">C3B55_00654</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.41" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00654"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="4">
        <location>
            <begin position="4"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="406" mass="43231" checksum="52668AEA1684958A" modified="2019-06-05" version="1">MRRVVITGLGIVSCLGNDKKTVTASLRTSRPGIRFNPEYAKMGLRSQVSGSIDLPLEKLIDRKIYRFIGHAAAYAYLTMKDAIADSGLSEDKVSNIRTGLIAGSGGASTLNQMEALDILREKGVKRVGPYRVTRTMGSTVSACLATPFQIKGVNYSISSACATSAHCIGTAVEQIKLNKQDIVFAGGGEEEHWSQSFLFDAMGALSTQYNKTPESASRAYDSKRDGFVIAGGGGMIVVEELEHALVRGAKIYAEIVGYGATSDGYDMVVPSGEGAIRCMQMAMSTISAPIDYLNTHGTSTSVGDAKEIECVRAVFGDKIPSISSTKSLSGHSLGAAGVHEIVYCLLMMENNFMAGSANIEEIDPVVANMPILTKTVENVKIDTVMSNSFGFGGTNATLVLKRWTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMQ7</accession>
    <name>A0A481QMQ7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_01004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01004"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="59" mass="6404" checksum="E912AFC03D914361" modified="2019-06-05" version="1">MSVLADFVCQLVIMSVKGAAITVLVIMFLAEAFFSKNIIFIIYSLALAITLAWLTSSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMR4</accession>
    <name>A0A481QMR4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14 15">UvrABC system protein B</fullName>
            <shortName evidence="14">Protein UvrB</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Excinuclease ABC subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 19" type="primary">uvrB</name>
        <name evidence="19" type="ORF">C3B55_00807</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12 14 15">Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The beta-hairpin motif is involved in DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 15">Belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00807"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00204">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024759">
        <property type="entry name" value="UvrB_YAD/RRR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="PTHR24029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12344">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00631">
        <property type="entry name" value="uvrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 14 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 14 15" id="KW-0228">DNA excision</keyword>
    <keyword evidence="9 14 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 14 15" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14 15" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="25"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="429"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="16">
        <location>
            <begin position="632"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Beta-hairpin" evidence="14">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="QAX82120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="671" mass="76680" checksum="ECA3187F321C4427" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMR5</accession>
    <name>A0A481QMR5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00571"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="38" mass="4155" checksum="27D8F25AB088279C" modified="2019-06-05" version="1">MKCTKAAMVICLQQMPVLLLSPAFIDTSSQFALKLAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMW9</accession>
    <name>A0A481QMW9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Adenylyl-sulfate kinase</fullName>
            <ecNumber evidence="2 3">2.7.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">APS kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">ATP adenosine-5'-phosphosulfate 3'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Adenosine-5'-phosphosulfate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 4" type="primary">cysC</name>
        <name evidence="4" type="ORF">C3B55_00840</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Catalyzes the synthesis of activated sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 2 3">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.25" evidence="2 3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00840"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00205"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Phosphoserine intermediate" evidence="2">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="199" mass="22529" checksum="86B58F4541B0D92A" modified="2019-06-05" version="1">MTNHNLRRVQNIHRQILSVTREDREKLNGHKGKVIWFTGLSGSGKSTLANALELELHAKNYRTYILDGDNLRHGLSRDLGFTDIDRAENIRRIAEVAKLMMDAGLTVLTAFISPFRRERQMAKELIGNDRFVEVYVSTTLEVCEKRDVKGLYKKARAGQLLNFSGVGSPYEVPVEATLEIDTAHINLNLALELLLDIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMX6</accession>
    <name>A0A481QMX6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">dTDP-4-dehydrorhamnose reductase</fullName>
            <ecNumber evidence="5 7">1.1.1.133</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rfbD</name>
        <name evidence="9" type="ORF">C3B55_00853</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57510"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="1.1.1.133"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the dTDP-4-dehydrorhamnose reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.133" evidence="5 7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00853"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="UniPathway" id="UPA00281"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009243">
        <property type="term" value="P:O antigen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="PTHR10491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01214">
        <property type="entry name" value="rmlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD_sub_bind" evidence="8">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="298" mass="32306" checksum="7D77AEF508DE35D8" modified="2019-06-05" version="1">MKILLFGKNGQIGWELQRSLAPLGQVLVLDSKSQNYCGDLNNLYGLAATVRQCAPNIIVNAAAYTSVDKAESEPAKALRINAEAPAVLATEALKLNALLVHYSTDYVFAGTGNIPWLENDPVGPLSVYGITKLKGERAIQTSGCAHLIFRTSWVYAARGNNFAKTMLRLAIERDSLNVIDDQFGTPTGADLLADVTAQAIRTIGLNQQLSGIYHLAAAGETTWYRYACFVLEQAQAAGVQLKVEPANVSAITTAAYPTPAKRPGNSRLNTQKLQNVLSLHLPKWQEGVELMVSKHSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMX8</accession>
    <name>A0A481QMX8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpoE</name>
        <name evidence="6" type="ORF">C3B55_00728</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00728"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014286">
        <property type="entry name" value="RNA_pol_sigma70_RpoE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02939">
        <property type="entry name" value="RpoE_Sigma70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="4">
        <location>
            <begin position="27"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="5">
        <location>
            <begin position="131"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="193" mass="22384" checksum="D7BADF84F0FEFB4D" modified="2019-06-05" version="1">MLAKAEDQQLVDCVQRGDKRAFDLLALKYQHKILGLIVRFVHDTHEAQDVTQEVLVRAYCALGNFRGDSAFYTWLYRIAINAAKNYLVSNRRHLLGIDISLEHTEFYDSNYNLKDLESPERALLRDEMKGAVYQAIQQLPEDSRTALILREFSFLSYEDIANIMQCPMGTVRSRIFRARELIDKALQPLLQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMY8</accession>
    <name>A0A481QMY8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA ligase</fullName>
            <ecNumber evidence="8">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">ligA</name>
        <name evidence="10" type="ORF">C3B55_00858</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00858"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00114">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033136">
        <property type="entry name" value="DNA_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNAligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004149">
        <property type="entry name" value="Znf_DNAligase_C4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="DNA_ligase_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03119">
        <property type="entry name" value="DNA_ligase_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="SSF52113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01056">
        <property type="entry name" value="DNA_LIGASE_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="BRCT" evidence="9">
        <location>
            <begin position="706"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="8">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="32"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="81"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="782" mass="86470" checksum="F49DBA5DC826A154" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMZ9</accession>
    <name>A0A481QMZ9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="5 15">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">lpdG</name>
        <name evidence="18" type="ORF">C3B55_00659</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="13 15">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="15">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="5 15"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00659"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13 15" id="KW-0274">FAD</keyword>
    <keyword evidence="6 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 13 15" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 15 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11 15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="17">
        <location>
            <begin position="6"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="16">
        <location>
            <begin position="353"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="188"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="325"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="14">
        <location>
            <begin position="49"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="QAX81984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="481" mass="50875" checksum="1E709CE90D8047EA" modified="2019-06-05" version="1">MSQKIDVVVIGAGPGGYVAAIKAAQLGLSTVCIEKYTNEEGRLALGGTCLNVGCIPSKALLDSSRKFYEARDSFAIHGISHTSIAIDVPAMVGRKANIIKSLTFGVTALFKANGVVSIQGHGKLISGKKVEITKPDGSVKVIEADNIVLAPGSRPISIPLAIIDHNVIVDSTDALEFQTIPKRLGVLGAGAIGLELGSVWCRLGARVTILEAMDTFLMTADAAVSQEAYKALTEQGLDIRLGARVVSLTVNDKEVIVTYTDQEGKQTIIFDKLIVAVGRSPMTIDLLASDSGVNIDDRGFIQVDDYCATTVPGVYAIGDVVRGMMLAHKASEEGIMVVERIKGHKTKINYDLIPSIIYTHPEIAWVGKNEQQLKAKGAKVNVGIFPFIASGRAIAANDTHGFVKVIADVKTDRVLGVHVIGSSAAELVQQGAIGMEFGTSAEDLSKIVFPHPTLSEALHEAALAVNGGAIHIANRNKLQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN01</accession>
    <name>A0A481QN01_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RraB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00866"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036701">
        <property type="entry name" value="RraB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009671">
        <property type="entry name" value="RraB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06877">
        <property type="entry name" value="RraB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89946">
        <property type="entry name" value="SSF89946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RraB" evidence="1">
        <location>
            <begin position="10"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="113" mass="12787" checksum="54AD1DFCE82D9A74" modified="2019-06-05" version="1">MITVYQEDISTNVLRRMKEGGFDFSRFHPIEFYAIFPDEELARRAADRFRGESLNAQVSACDNGAWHLELSKIMFATYDGIGDFEQDFEAVVGPLGGIMEGWGVKQEVRGLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN32</accession>
    <name>A0A481QN32_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Peptide deformylase</fullName>
            <shortName evidence="4">PDF</shortName>
            <ecNumber evidence="4">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 4">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">def</name>
        <name evidence="6" type="ORF">C3B55_01008</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="4">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01008"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="186" mass="21260" checksum="6BAD1C2CDD5B9BAE" modified="2019-06-05" version="1">MLICLYFIYAVITFMAILNILKFPDSRLRTIAKPVTMVNNKVIQLIDDMLETMYKAMGIGLAATQVNVHQRVIVMDLSEDQSQPLVFINPEFEPLSKKRNTYQEGCLSVPEFYENIERPVRVKVQALDRNGKLYGLIADGLLAVCIQHECDHLNGKLFVDYLSTPKRAMIKKKLQKSITSKFTAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN39</accession>
    <name>A0A481QN39_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="6">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">suhB</name>
        <name evidence="7" type="ORF">C3B55_00967</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00967"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052832">
        <property type="term" value="F:inositol monophosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052833">
        <property type="term" value="F:inositol monophosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022337">
        <property type="entry name" value="Inositol_monophosphatase_SuhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01959">
        <property type="entry name" value="SBIMPHPHTASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="271" mass="29793" checksum="4D356267AD17BDC6" modified="2019-06-05" version="1">MQPMLNIAMRAARSASELIFRSIERIDILKLNEKDAKNYASELDYAAEQKIIDALRKVYPTHGILGKGTGLHKGNSEDEGYLWIIDPLDSTMNFLRGIPHSAVSIACKYRSRLEHAVVLDPVRQEEFTASRGCGAQLNGRRLRVSSRTSLHGALLGTNFSFYYNQIDNLKNYLGMFHALTNQTAGIHCAGAASLDLAYVAAGRLDAFWELGLSEWKMAAGALLIQEAGGLVSDFTGSHNFLEKGHLVAGNTKCFKAVLTIIQSYLPSSLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN96</accession>
    <name>A0A481QN96_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease P protein component</fullName>
            <shortName evidence="5">RNase P protein</shortName>
            <shortName evidence="5">RNaseP protein</shortName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein C5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rnpA</name>
        <name evidence="6" type="ORF">C3B55_01019</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RnpA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01019"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00227">
        <property type="entry name" value="RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000100">
        <property type="entry name" value="RNase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33992">
        <property type="entry name" value="PTHR33992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00825">
        <property type="entry name" value="Ribonuclease_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00188">
        <property type="entry name" value="rnpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="117" mass="13584" checksum="CB7A3E437ECD9818" modified="2019-06-05" version="1">MTKNFGREKRLLTAQNFTAVFASPTNTIPGNSTLLLARKNDLNYPRLGLVISKKNVKLSVKRNRLKRLIRETFRLHQDKLIGWDIVIVARKGLGDIGNYKLIQHFGQLWKRLSRTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNC3</accession>
    <name>A0A481QNC3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protoheme IX farnesyltransferase</fullName>
            <ecNumber evidence="9">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Heme B farnesyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">cyoE</name>
        <name evidence="10" type="ORF">C3B55_00974</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate + Fe(II)-heme o</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00974"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PTHR43448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="295" mass="32750" checksum="4A0AB642D0BFB4FD" modified="2019-06-05" version="1">MPLKHFIQITKPGIIFSNVLSVAGGFFLASKGHVDLAIFLAVMIGTSLVVASGCVFNNYIDRDIDLKMERTKNRALVHGLLSMKLVLTYATVLGIVGIMLLYIAANLLAALFALIGFVIYVCLYSLYLKRKSVHGTLAGSLSGAMPPVIGYVAVTNHFDIAALVLMVMFSLWQMPHSYAIAIFHFNDYLAASIPVLPVKRGIQVTKKYILLYIFGFLMATLMLTFSGYTSINYLVAAATMGTYWLYIAWTGYKAADDTIWARKLFVFSIFTITTLSVMMSLDFQIPKELLLTYEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QND1</accession>
    <name>A0A481QND1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Cell division protein ZipA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">zipA</name>
        <name evidence="12" type="ORF">C3B55_00859</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Essential cell division protein that stabilizes the FtsZ protofilaments by cross-linking them and that serves as a cytoplasmic membrane anchor for the Z ring. Also required for the recruitment to the septal ring of downstream cell division proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with FtsZ via their C-terminal domains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">Localizes to the Z ring in an FtsZ-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the ZipA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00859"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090529">
        <property type="term" value="P:cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00509">
        <property type="entry name" value="ZipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011919">
        <property type="entry name" value="Cell_div_ZipA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007449">
        <property type="entry name" value="ZipA_FtsZ-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036765">
        <property type="entry name" value="ZipA_FtsZ-bd_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38685">
        <property type="entry name" value="PTHR38685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04354">
        <property type="entry name" value="ZipA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00771">
        <property type="entry name" value="ZipA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64383">
        <property type="entry name" value="SSF64383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02205">
        <property type="entry name" value="septum_zipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 8 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 8 10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 8 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="ZipA_C" evidence="11">
        <location>
            <begin position="146"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="288" mass="32426" checksum="FE41EA1EB82CD831" modified="2019-06-05" version="1">MKIGLREWLIIIIAIIIVAIIFNIWRCMCSGKGKLKFRLKRNLSNLSTDSSTELLLPACALDTHIKPQLDEHDLPSLSASVREVCKPSFKHKNRYGIQVTKSHNGNFNLYVNESLNLNGQNSTFYDENVSKQAAMPRQSVNTQSITEEVLVISVISRDAAGFKGPVLLQNILESGLRFGKMNIFHCHENISGNSKVLFSMANAVKPGTFDLNDIDLFSTPAVSFFLGLPSSCHPKQAFNMMVAAARKLSQELNGELKDDQRSVMTVQTIEHYRQRIVEFERRALTQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QND5</accession>
    <name>A0A481QND5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">1-deoxy-D-xylulose 5-phosphate reductoisomerase</fullName>
            <shortName evidence="7">DXP reductoisomerase</shortName>
            <ecNumber evidence="7">1.1.1.267</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">1-deoxyxylulose-5-phosphate reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">2-C-methyl-D-erythritol 4-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">dxr</name>
        <name evidence="11" type="ORF">C3B55_00765</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.267"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DXR family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.267" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00765"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00092"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030604">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00183">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003821">
        <property type="entry name" value="DXP_reductoisomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013644">
        <property type="entry name" value="DXP_reductoisomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013512">
        <property type="entry name" value="DXP_reductoisomerase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026877">
        <property type="entry name" value="DXPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036169">
        <property type="entry name" value="DXPR_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30525">
        <property type="entry name" value="PTHR30525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08436">
        <property type="entry name" value="DXP_redisom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02670">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13288">
        <property type="entry name" value="DXPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006205">
        <property type="entry name" value="Dxp_reductismrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69055">
        <property type="entry name" value="SSF69055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00243">
        <property type="entry name" value="Dxr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="5 7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DXP_reductoisom" evidence="8">
        <location>
            <begin position="7"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="DXP_redisom_C" evidence="9">
        <location>
            <begin position="149"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="DXPR_C" evidence="10">
        <location>
            <begin position="269"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="396" mass="42620" checksum="0A0CEFC5F051C5D9" modified="2019-06-05" version="1">MNRLQQITVLGATGSVGLSTLDVIARHPNLYQVFALTGFTRLSELLALCIRYLPRFAVVPEVELARGMQQHLQAAGLATQVLVGEEGLCQVSVDTEVDTVVAVIVGAAGLRPTLAAVNAGKKILLANKEALVMSGALFMQAVRKSGAVLLPLDSEHNAIFQCMPNNFASGLSQVGVRRILLTASGGPFWPNPSVDLEHVSPDQACAHPNWSMGRKISVDSASMMNKGLELIEACWLFDAKSDQIEVVIHPQSVIHSLVDYVDGSMLAQLGNPDMRTPIANALAWPERIDSGVAPLDLLAIARLDFEMPDEQRFPCLCLARQAAEIGNSAPVILNAANEVAVTAFLDGRIRFPQIASIIEDVLNLEPVVVVDDLGAVFEADARARTLAAQWLNRNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNF9</accession>
    <name>A0A481QNF9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rpmF</name>
        <name evidence="7" type="ORF">C3B55_00880</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00880"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00340">
        <property type="entry name" value="Ribosomal_L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_L32p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044957">
        <property type="entry name" value="Ribosomal_L32p_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35534">
        <property type="entry name" value="PTHR35534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="60" mass="6934" checksum="205D104F0D2EA5F0" modified="2019-06-05" version="1">MAVQQNKKSRSARNMRRSHDALKANTLSIEKTTGEVYRRHHVSLEGVYRGRRVIIKGTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNG6</accession>
    <name>A0A481QNG6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="8">SDH</shortName>
            <ecNumber evidence="3 8">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">aroE</name>
        <name evidence="12" type="ORF">C3B55_01005</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="3 8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01005"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate_dh_N" evidence="10">
        <location>
            <begin position="6"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate_DH" evidence="9">
        <location>
            <begin position="116"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="SDH_C" evidence="11">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate binding" evidence="8">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="126"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="150"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="268" mass="29191" checksum="49071FD43F2F87E1" modified="2019-06-05" version="1">MDRYVVFGNPISHSKSPLIQRMFAEQTGKQLHYSTMLAPLENFTGCVNEFFRQGRGANITMPFKEHAYRLANRLTKRAQRTGAVNTLSKLVDGTLFGDNTDGVGLVRDLTVNAGFSLRGKHILLLGAGGAVRGVLEPLLAKQPTLLILANRTVEKAKLLVSLFNDINSVYACGFDSLLEPVDVIINATSVSMSEIVPPISGSLVKPGKTICYDMMYGKQSTAFCCWANKQGAAVVMDGLGMLVEQAAESFFFWHGVRPDTASVLAELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNN3</accession>
    <name>A0A481QNN3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator BolA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00812"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="SSF82657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="95" mass="10969" checksum="D942FB691A42CEE2" modified="2019-06-05" version="1">MRKLIETALATFSPQYLSVLDISHMHSRGLQTHFKIVLVSLQFNRLDRVKRHQIVYATLGNLMSEFHALALHIYTPEEWLTINAAPPSPICARYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNV1</accession>
    <name>A0A481QNV1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane protein TerC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00990"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005496">
        <property type="entry name" value="Integral_membrane_TerC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03741">
        <property type="entry name" value="TerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="244" mass="27220" checksum="856C2581ABD36146" modified="2019-06-05" version="1">MEWLTNPEIWIAFFTLTTLEIVLGIDNIIMISILVSNMPKHMQTRTRIFGLALAMITRILLLLSITWVMQLTVDLFVIFGHGISSRNLILFFGGQFLLWKSSQEIYHTLTGIDKARNKPKGASNKFIYTIIQIAIIDIVFSLDSVITAVGIVSHVPVMIAAIIVAILMMMLGVGTISKFIDKHPSLKILALSFLIVVGVVLIAESFNVHIPKGYVYFTITFSLAVEIINIKMRATIAKKNSTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNY3</accession>
    <name>A0A481QNY3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lipid A biosynthesis lauroyl acyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">htrB_2</name>
        <name evidence="6" type="ORF">C3B55_01011</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01011"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07984">
        <property type="entry name" value="LPLAT_LABLAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004960">
        <property type="entry name" value="LipA_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30606">
        <property type="entry name" value="PTHR30606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03279">
        <property type="entry name" value="Lip_A_acyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026649">
        <property type="entry name" value="MsbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="295" mass="33083" checksum="B67439195C1CC58F" modified="2019-06-05" version="1">MEKLKGALLVGALRLFALLPWRAVQIIGMMIGWIMWKTHNRSREIVRINLSKCFPGMNSDARELLVGQSLMDAGKSMTESAYVWSWPAQRSINLIHEVEGLEVLHAALASGKGVVGITSHLGNWEVLNYFYCSQCKPIIFYRQPKLKAVDKLLQKQRTQLGNRVVASTTKGILSVIKEVRKGGQVGITADPEPAESAGIFVPFFATQALTSKFVPSMLADYKAVGVFLHALRLPNGSGYRIILEAAPEDMYSTDTATSCAAMSKVMERYIDAYPNQYMWSMKRFKKRPPGEERWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QP37</accession>
    <name>A0A481QP37_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00921"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007922">
        <property type="entry name" value="DUF721/UPF0232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05258">
        <property type="entry name" value="DciA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="157" mass="17809" checksum="5D07AE5AFAAF17A4" modified="2019-06-05" version="1">MAFCPPMARSLKVLLSEAKPLIAIFSHAQRISYLQRLAESQLQPAARKHCHVASWHEGNLLFIVTDGHWATRLRYQQKYLQRQLMIFTEFASLTHIQFKVQPPNMQQNLVEHNKMGLSDSAAKTIQSTACSISNPSLRVALERLASRANNPCSTDYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK12</accession>
    <name>A0A481QK12_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00021"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="29" mass="3325" checksum="F1F9EB9BADBEA8E1" modified="2019-06-05" version="1">MHAFFIASLKHHIMGVVFYVEYAAGKKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK16</accession>
    <name>A0A481QK16_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanosine-3',5'-bis(diphosphate) 3'-diphosphatase</fullName>
            <ecNumber evidence="3">3.1.7.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">spoT</name>
        <name evidence="8" type="ORF">C3B55_00026</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine 3',5'-bis(diphosphate) + H2O = diphosphate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14253"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:77828"/>
            <dbReference type="EC" id="3.1.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; ppGpp biosynthesis; ppGpp from GDP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the relA/spoT family.</text>
    </comment>
    <dbReference type="EC" id="3.1.7.2" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00026"/>
    <dbReference type="UniPathway" id="UPA00908">
        <property type="reaction ID" value="UER00886"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015970">
        <property type="term" value="P:guanosine tetraphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TGS_RSH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Beta-grasp_dom_sf"/>
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        <property type="entry name" value="HD/PDEase_dom"/>
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        <property type="entry name" value="NT_sf"/>
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    </dbReference>
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        <property type="entry name" value="RelA/SpoT_AH_RIS"/>
    </dbReference>
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        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033655">
        <property type="entry name" value="TGS_RelA/SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19296">
        <property type="entry name" value="DUF5913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00691">
        <property type="entry name" value="spoT_relA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="6">
        <location>
            <begin position="45"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="7">
        <location>
            <begin position="387"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="5">
        <location>
            <begin position="627"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="701" mass="79216" checksum="22275DB71D99C69B" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK80</accession>
    <name>A0A481QK80_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00056"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="58" mass="6904" checksum="55719CC729270B2B" modified="2019-06-05" version="1">MLIMLFNYLRAVKIFNIICEDIATCFITKLDADKFLWINANPGLIDEYNYLAYNMRIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK93</accession>
    <name>A0A481QK93_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putrescine transport system permease protein PotH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">potH</name>
        <name evidence="6" type="ORF">C3B55_00061</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00061"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="88"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="306" mass="34454" checksum="8DFB9E2BB35B11C4" modified="2019-06-05" version="1">MNMMKLQHQMQRIMPDSQYIVIGIPFFWLLLFFALPFFIVLRISFAEAAVAIPPYTEIYTFVEQKLQLVLNLGNYALLGNDELYIAAYLGSLKIAFFSTILCLLIGYPMAYAISNARKDVQTVLVLLIMMPTWTAILIRVYAWMGILSTNGLLNGFLMSIGLINEPLQILNNNFAVYIGIVYSYLPFMILPLYANLVRHDPSLLEAASDLSSSTFNSFWKITVPLSKNGIIAGCMLVFIPVVGEFVIPELLGGPETLMIGKVLWQEFFNNRDWTVASALAVVMLVLLIMPIILFNRSQAKEIEGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKB0</accession>
    <name>A0A481QKB0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor RpoH</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">RNA polymerase sigma-32 factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">rpoH</name>
        <name evidence="9" type="ORF">C3B55_00080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the RNA polymerase core enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sigma-70 factor family. RpoH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00080"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00961">
        <property type="entry name" value="Sigma70_RpoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012759">
        <property type="entry name" value="RNA_pol_sigma_RpoH_proteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02392">
        <property type="entry name" value="rpoH_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2 6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="7">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="8">
        <location>
            <begin position="252"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="6">
        <location>
            <begin position="54"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="6">
        <location>
            <begin position="78"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="284" mass="32457" checksum="AA464F365249126D" modified="2019-06-05" version="1">MITSLQPAHALVPGANLEAYLHTVNSISLLTPEKERELAESFYYEQDLGAARQIVLAHLRFVVHIARSYSGYGLAQADLIQEGNVGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEFILRNWRIVKVATTKAQRKLFFNLRGQKKRLAWLSNEEVHRMAESLGVETHEVREMESRLTGHDMAFDPAAEADDDNAFQSPVNYLEDHRYDPARQLEDADWSDNSNQNLHAALKALDDRSRDILCQRWLAEEKATLHDLAQKYNVSAERIRQLEKSAMNKLKLSIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKG8</accession>
    <name>A0A481QKG8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 15" type="primary">rpe</name>
        <name evidence="15" type="ORF">C3B55_00121</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10 13">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 10 11">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10 11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00121"/>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="144"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="177"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="199"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 12">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13 14">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX81491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="223" mass="24257" checksum="44F6FF8BBF02366E" modified="2019-06-05" version="1">MQPFVIAPSILSADFARLGEEVDKILAAGADFVHFDVMDNHYVPNLTIGPMVCAALCKYGITAPIDAHLMVSPVDRIVSDFIETGATYITFHPEATLHVDRTLQLIREGGCKAGLVFNPATTLSLLEYVIDKIDMVLLMSVNPGFGGQKFIPGIINKLREARMLIDASGRDIRLEIDGGVNINNIREIAVAGADTFVVGSAIFKAPNYQEVINKMRTELALVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKK4</accession>
    <name>A0A481QKK4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00155"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="87" mass="10167" checksum="BD2F7DF06DE316F2" modified="2019-06-05" version="1">MVLGESIAVWRPVWLAPIRLILLRFLFHNRFADVILSIYTMPTTQLYLVISYLFIIKCAPLDIITCLLSGVLWCFHFRFKDLAVSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKL1</accession>
    <name>A0A481QKL1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methionine biosynthesis protein MetW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metW</name>
        <name evidence="1" type="ORF">C3B55_00083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00083"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010743">
        <property type="entry name" value="Methionine_synth_MetW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07021">
        <property type="entry name" value="MetW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02081">
        <property type="entry name" value="metW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="202" mass="23204" checksum="2C62CF46F1153718" modified="2019-06-05" version="1">MRADLEIIQDWIPSDSRILDLGCGDGELLSWLRDNKHVTGYGLENDPDNIAKCITKGVNVIEQDLDKGLINFTSNSFDIVVMTQALQAVHYPDRILDEMLRVGRQCIITFPNFGHWRCRWYLATKGCMPVSDFLPYTWYNTPNIHFCTFEDFETLCSKRKAKVINRLAVDQQHHHGWASKLWPNLLGEIGIYHVSSPGLTDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKN1</accession>
    <name>A0A481QKN1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 10">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="2 7 10">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">pgk</name>
        <name evidence="11" type="ORF">C3B55_00103</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 10">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="2 7 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00103"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="21"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="340"/>
            <end position="343"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="390" mass="40829" checksum="6673E2210DDA7DCB" modified="2019-06-05" version="1">MTILKMTDLDLQGKRLLIRADFNVPFKNGVVTSDTRILASLPTIKLALEKGAAVMVCSHLGRPTEGEFSSENSLKPVAEYLSKALGLDVLLLANYLGGVNVKPGEIVLFENVRFNKGEKKNSDKLAKQYAALCDVFVMDAFGTAHRVESSTHGVAKFAKVAAAGPLLIAELDFLSRALASPVQPMTAIVAGSKVSTKLEALNSLSQICSQIIVGGCIANTFLAATGHPVGRSLFEPNLLVTARAIVAKVSVLLPADVVVAKKFAKNAEATIKLIADVTANDIILDIGPQTAASFAKLLKSSQTILWSGPVGVCEFDQFGCGTKVLAKAIAESSAFSIVGGGDTLAAIHKYSVADQISCVSTGGGAFLELVAGKVLPAVEVLETRAKFKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKX6</accession>
    <name>A0A481QKX6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenosylmethionine-8-amino-7-oxononanoate aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">7,8-diamino-pelargonic acid aminotransferase</fullName>
            <shortName evidence="4">DAPA AT</shortName>
            <shortName evidence="4">DAPA aminotransferase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">7,8-diaminononanoate synthase</fullName>
            <shortName evidence="4">DANS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Diaminopelargonic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">bioA</name>
        <name evidence="6" type="ORF">C3B55_00094</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate</text>
            <dbReference type="Rhea" id="RHEA:16861"/>
            <dbReference type="ChEBI" id="CHEBI:16490"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="EC" id="2.6.1.62"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.62" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00094"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="116"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="320"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM" evidence="4">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="468" mass="52912" checksum="EFEEF28EDBF31AF1" modified="2019-06-05" version="1">MNLNNQWMQRDLAVLWHPCTQMKDHQQLPLIPIKRGEGVWLEDFEGKRYIDAISSWWVNLFGHANQRINKRIKDQIDQLEHVILAGFSHQPVIELSERLIEITPESLTRCFYADNGSSCIEVALKMSFHYWLNRGLPKKKRFITLTNSYHGETIAAMSIGDVPLFTETYKALLLDSIKVPSPDCYLRPDGISWEEHSRNMFLAMEQALAENHNTVAAVIIEPLIQGAGGMRMYHPVYLELLRESCDRYNVHLIHDEIAVGFGRTGSMFACEQACICPDFLCLSKALTGGYLPLAAVVTTEDVYSAFYDDYQTLRAFLHSHSYTGNPLACAAALATLDIFKEDNVIVKNKALAQCMTKATANLIDHPHISEVRQTGMIMAIEMVQDKTNKAAYPWQERRGLKVFKHALKRGALLRPLGSVVYLLPPYVITPEQISFLVEIASEGIDIATNSKVSVAMSKDFYPNFRDPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKY7</accession>
    <name>A0A481QKY7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fructose-1,6-bisphosphate aldolase</fullName>
            <shortName evidence="8">FBP aldolase</shortName>
            <ecNumber evidence="8">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fda</name>
        <name evidence="9" type="ORF">C3B55_00104</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the class II fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00104"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00947">
        <property type="entry name" value="TBP_aldolase_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006412">
        <property type="entry name" value="Fruct_bisP_Calv"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001359">
        <property type="entry name" value="F_bP_aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00167">
        <property type="entry name" value="cbbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01521">
        <property type="entry name" value="FruBisAldo_II_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00602">
        <property type="entry name" value="ALDOLASE_CLASS_II_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00806">
        <property type="entry name" value="ALDOLASE_CLASS_II_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="6">
        <location>
            <begin position="233"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="6">
        <location>
            <begin position="275"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="348" mass="37701" checksum="D79A716B37C5585C" modified="2019-06-05" version="1">MALISMRQMLDHAAEFGYGIPAFNINNLEQMRAIMESADKTDSPVIVQASAGARKYAGAPFLRHLILAAIEEFPHIPVCMHQDHGTSPDVCQRSIQLGFSSVMMDGSLSEDGKTPTDYEYNVRVTQQTVAMAHACGVSVEGELGCLGSLETGTACEEDGIGAQGILNHSQMLTDPDEAADFVKKTQVDALAIAIGTGHGAYKFTKPPTGEVLAIDRIKEIHKSIPNTHLVMHGSSSISQEWLAIINQYGGKIKKTYGVPVDKIVEGIKHGVRKVNIDTDLRLASTGAMRSLMATKPSEFDPRKFFSATIVAMRDVCIARYEAFGTAGNASKIKPISLEAMHQYYLQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKY9</accession>
    <name>A0A481QKY9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00048"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="65" mass="7261" checksum="C8B149E41F6106AB" modified="2019-06-05" version="1">MSRFTLCVWVVWLMSITSDAVFKVLILGYLIKGVHISAVNLGVSSSILTLTLISQQHLSYFMSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKZ8</accession>
    <name>A0A481QKZ8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Multifunctional CCA protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">CCA-adding enzyme</fullName>
                <ecNumber evidence="10">2.7.7.72</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">CCA tRNA nucleotidyltransferase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="10">tRNA CCA-pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="10">tRNA adenylyl-/cytidylyl-transferase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="10">tRNA nucleotidyltransferase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="10">tRNA-NT</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">2'-nucleotidase</fullName>
                <ecNumber evidence="10">3.1.3.-</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">2',3'-cyclic phosphodiesterase</fullName>
                <ecNumber evidence="10">3.1.4.-</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">Phosphatase</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">cca</name>
        <name evidence="12" type="ORF">C3B55_00115</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Also shows phosphatase, 2'-nucleotidase and 2',3'-cyclic phosphodiesterase activities. These phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Magnesium is required for nucleotidyltransferase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="10">Nickel for phosphatase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer. Can also form homodimers and oligomers.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">Comprises two domains: an N-terminal domain containing the nucleotidyltransferase activity and a C-terminal HD domain associated with both phosphodiesterase and phosphatase activities.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="10"/>
    <dbReference type="EC" id="3.1.3.-" evidence="10"/>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00115"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052928">
        <property type="term" value="F:CTP:3'-cytidine-tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CTP:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004112">
        <property type="term" value="F:cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016437">
        <property type="term" value="F:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01261">
        <property type="entry name" value="CCA_bact_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01262">
        <property type="entry name" value="CCA_bact_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012006">
        <property type="entry name" value="CCA_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000813">
        <property type="entry name" value="CCA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-0533">Nickel</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0692">RNA repair</keyword>
    <keyword evidence="9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="HD" evidence="11">
        <location>
            <begin position="228"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="409" mass="46694" checksum="9B8BBAFD31580241" modified="2019-06-05" version="1">MKIYKVGGSVRDRLLGIKSTDIDRVVVGATTKEMLANGYKPVGTDFPVFLDPKNGDEYALARTEYKSGRGYGGFIFHTSPKVTLEEDLIRRDLTINAMAEDDDGNLIDPYNGQRDLEARTLRHVSLAFAEDPLRVLRVARFAARYKRLGFIIAPQTLELMRQISESKELTTLTPERSWKEISRALMENQPQVFIQVLRDCNALKSLTPELNTLFGITQPTANQSEIDIGTHTLNVLEQTALHQQPLTIRWACLLYNLNKDLIPMDKLPQHLTCKHINFKLIKAINNRFKVPKDCQELSLLVNQYHTYGRRALELKPSTLLELLQSFDIYRRPQRFEAFIVSCEMNARGCTDFKQRSYPQAEYLRGAAQAAREVPISHLLNQALQGKELGEALKHERLKALHAYKEQQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL11</accession>
    <name>A0A481QL11_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Farnesyl diphosphate synthase</fullName>
            <ecNumber evidence="3">2.5.1.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ispA</name>
        <name evidence="3" type="ORF">C3B55_00317</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00317"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:geranyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="295" mass="31706" checksum="B9CB06C74D01925A" modified="2019-06-05" version="1">MIDAYQASSQARVNAALEPLFVAPSPEMNRLYEIMRYSVMNGGKRIRPSLAYAACEALGAPAELANGAACAVELIHAYSLVHDDLPAMDDDDLRRGQPTPHKAFDEAYAILAGDGLQSLAFSALLDPRLNSANAEISMRMVTTLALAAGPAGMVGGQAIDMNSINLKPDRQALEHMHRHKTGALIEATTHLGALASGCAETTQLMALQAYSQAIGLAFQVQDDILDIESHTAILGKRQGADIARNKLTYPMLLGLEAAKVYVLELRDQALDALISFDAAAESLRSLARYIVARRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL25</accession>
    <name>A0A481QL25_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fructose-1,6-bisphosphatase class 1</fullName>
            <shortName evidence="5">FBPase class 1</shortName>
            <ecNumber evidence="5">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">fbp</name>
        <name evidence="9" type="ORF">C3B55_00171</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">Belongs to the FBPase class 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00171"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="PTHR11556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBPase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="FBPase_C" evidence="8">
        <location>
            <begin position="201"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="336" mass="37312" checksum="2DD8452C914D971B" modified="2019-06-05" version="1">MSRVILSRYLIEQARSNNTPDDLCFLIEAVARACKEISQVVSKGALGGILGSMGTENVQGEEQKKLDVISNEILLKANEWGGHLAAMASEEMGHAYQIPRKYPKGANLLVFDPLDGSFNIDITATVGTIFSVLHCPNEYLSQNEVLNEKAFLQPGTEQVAAGYAIYGPQTMLILTVGDGVQGFTLDREIGSFVLTHEDMNIPASTQEFAINMSNQRYWEEPVIRYVGELIAGQDGPLEKNFNMRWVAAMVADVHRILIRGGLFMYPRDRREPSKPGKLRLMYEASPMSFLVEQAGGASTNGYQRILDIQPKGLHQRVAVFLGSKEEVERITAYHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL44</accession>
    <name>A0A481QL44_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00247"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="48" mass="5370" checksum="3975D9A2F3F5CF24" modified="2019-06-05" version="1">MFNYAEYISKIEPYAALAAIIFLLTNSVCSDIAFKISAIKLKLMIDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL52</accession>
    <name>A0A481QL52_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rplW</name>
        <name evidence="6" type="ORF">C3B55_00279</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Contacts protein L29, and trigger factor when it is bound to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00279"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_L23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="PTHR11620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="99" mass="11146" checksum="B9C28665A89C51CD" modified="2019-06-05" version="1">MNQKCVFKFLLSPHVSEKATIMADKKGQFVFKVATDATKLEIKRAVESLFNVKAKRVTTINILGKSKRTARGLGKRSNWKKAMIFLQPGQDLDFSSKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL66</accession>
    <name>A0A481QL66_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">rpsG</name>
        <name evidence="10" type="ORF">C3B55_00273</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts proteins S9 and S11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00273"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_B">
        <property type="entry name" value="Ribosomal_S7_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005717">
        <property type="entry name" value="Ribosomal_S7_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01029">
        <property type="entry name" value="rpsG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S7" evidence="9">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="156" mass="17675" checksum="940E5EC1DDB586CF" modified="2019-06-05" version="1">MPRRRVAAKREVLDDPKYGSQILAKFMNHVMESGKKAVAERIVYGALEKVKERKNSDPLEVFKKALDAIAPLVEVKSRRVGGATYQVPVEVRPSRRNALAMRWLVDFARKRGEKSMALRLAGELLDAAEGKGIAVKKREDVHRMAEANKAFSHYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL71</accession>
    <name>A0A481QL71_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">dTDP-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="4 5">4.2.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rffG</name>
        <name evidence="7" type="ORF">C3B55_00152</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O</text>
            <dbReference type="Rhea" id="RHEA:17221"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="EC" id="4.2.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="4 5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00152"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01181">
        <property type="entry name" value="dTDP_gluc_dehyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="6">
        <location>
            <begin position="4"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="347" mass="39310" checksum="2E766AFF9202B219" modified="2019-06-05" version="1">MRILITGGAGFIGSALVRYLIQNTEHEVFNLDKLTYAGNLESLTSIALNSRYEFVQVDIINQATVSSILARFEPQAVMHLAAESHVDRSINRPSDFIQTNIVGTYSMLEATRAYWQTLTEPDKSVFRFHHISTDEVYGELNDADDPFTENKPYAPSSPYAASKSASDHLVRAWQRTYGLPVLLTNCSNNYGPFHFPEKLIPLVILNAIAGKPLLVYGDGLQIRDWLFVEDHARALLKVVTKGIVGETYNIGGHNEQKNIDVVHRICSLLEELTPQRTASIKNFTNLITFVKDRPGHDQRYAINASKIKNELGWVPEETFETGLRKTVQWYLDNLNWCRMIQEGSYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL98</accession>
    <name>A0A481QL98_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00167"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="71" mass="8276" checksum="0988E7E4F1722AE9" modified="2019-06-05" version="1">MLVFNSMVNSLLVIMSYLAYAVLTDYAAISKRNKLFYSLRIGTIPDSFNNVTKQHYHKCYVVYNSRIISQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLA8</accession>
    <name>A0A481QLA8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ubiB</name>
        <name evidence="11" type="ORF">C3B55_00177</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis [regulation].</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00177"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13972">
        <property type="entry name" value="UbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010232">
        <property type="entry name" value="UbiB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045308">
        <property type="entry name" value="UbiB_bact"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01982">
        <property type="entry name" value="UbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1" evidence="10">
        <location>
            <begin position="95"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="534" mass="62312" checksum="C52FE119803135E0" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLA9</accession>
    <name>A0A481QLA9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable nicotinate-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Deamido-NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Deamido-NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nicotinate mononucleotide adenylyltransferase</fullName>
            <shortName evidence="7">NaMN adenylyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">nadD</name>
        <name evidence="9" type="ORF">C3B55_00323</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the NadD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.18" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00323"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00244">
        <property type="entry name" value="NaMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00482">
        <property type="entry name" value="TIGR00482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="8">
        <location>
            <begin position="7"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="214" mass="24057" checksum="FA1D70DC26E78DA8" modified="2019-06-05" version="1">MAKRIGLLGGTFDPVHIGHMRSVLEVVDVLALDELRLMPCAQPLHRDKPQVSARQRFEMVHLAVEGIPLLVADARELTRDQPSYTIDILELIRTELAEDDQLFLLLGWDAFCVLPSWYRWQELLHYCHILVLQRQDVDNKLPDALCSLLATQSVNDPSAMTGPNGSIAFVWQTQLAVSATQIRQLLARGKSVRFLVPDAVLAYINAHGLYCALH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLB1</accession>
    <name>A0A481QLB1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber evidence="4">2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">C3B55_00393</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00393"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="285" mass="31060" checksum="2317A7A5EE93E61B" modified="2019-06-05" version="1">MRERFEERNQVSDAASLLPIDEHVEEGHDAEGHKVRYRGIYLLPNLFTTANLFAGFYSIINSMNAQGSLAAGDVASASKYFFFAAIAIFIAMVLDVLDGRVARMTNTQSTFGAEYDSLSDMVAFGVAPALLTFAWALGDMGRVGWMVAFIYVAGAALRLARFNTQVSTADKRYFIGLATPAAAGVLDGVVWAFSDYGIQGSRMSFLVALVVAAAGMLMVSNIKYSSFKQFDLKGRVPFVAILVVVLVFAVVFSDPPRILLLAFLIYATSGPIQYLLHLRRSKTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLB2</accession>
    <name>A0A481QLB2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">F0F1 ATP synthase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">C3B55_00004</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00004"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005598">
        <property type="entry name" value="ATP_synth_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03899">
        <property type="entry name" value="ATP-synt_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="136" mass="15421" checksum="9313C9CDDED39096" modified="2019-06-05" version="1">MEIRTLNTLPFHRLAVFPILLAQLVVLLIAALAIWYWYGVIAGYSGLYGGLMSFLPNIYFTHRVFRFSGAIAAQSIVRLFYTSEIKKLILTAFLFTLIFVGFQPLTPLALFGVFVLTQMVIWFAPLLMKVNLAKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLC6</accession>
    <name>A0A481QLC6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipopolysaccharide core biosynthesis protein RfaG</fullName>
            <ecNumber evidence="2">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rfaG</name>
        <name evidence="2" type="ORF">C3B55_00199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00199"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="185"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="374" mass="42556" checksum="E14366A3AEC0EFBC" modified="2019-06-05" version="1">MQLAFVLYKYFLFGGLQRDFMRIALECQRRGHKIRVYTLIWEGDVPPGFEVLKATVRAFFNHRRNEKFNAWIEADLAKHKVDRLVGFNKMPGLDIYYAADGCFEDKAQNLRHLLYKSFGRYKHFAKYESMVFAKDATTKILVISEVQQLLFIKHYNTPLVRFHLLPPGIAQDRRAPQNSTEIRKSFRKEFNVSDDDMLLVQIGSGFKTKGVGRSLKAVAALPFELKKRTRLFVIGQDNPKVFQLQSAILGLSDSVQFFKGRSDILRFLLGADLLIHPAYNENTGTVLLEALVAGLPVLASAICGYANYIAEANNGLVLDEPFKQAQLNQYLVYMLIDYAQRAAWGRNGLAYAETADLYSMPQHAADIILAELKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLC8</accession>
    <name>A0A481QLC8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">ATP phosphoribosyltransferase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">hisZ</name>
        <name evidence="8" type="ORF">C3B55_00232</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heteromultimer composed of HisG and HisZ subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">This function is generally fulfilled by the C-terminal part of HisG, which is missing in some bacteria such as this one.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00232"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA-synt_His" evidence="7">
        <location>
            <begin position="24"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="407" mass="44772" checksum="930F6FBED3CD7AF9" modified="2019-06-05" version="1">MVGRRDYRGNGVMVTVDRWLLPDGIEEVLSPEAECIEVARRQVLDLFQSWGYEFVMPPHIEYLESLLTSARYDLDLCIFKVIDPQSGRQMGFRADIAPQVARIDAHTLCRKGPSRLCYAGSVLHVQPRSLSSSRSPIQLGAELYGDTSLSSDVEVISLMLSMLQLVDVPDVHMDLGHVGIYRGLAHAAGLSNKVEQQLFIALQCKAIDKVITLTEDVPVDLADMLRALVNLCGGYEVLAVARKCLANAPAPVLTALDDILVIAERLLARFPELPLHFDLCELSGYHYQTGVVFAVFVPGIGQAIAQGGRYDYIGADFGRARPATGFSADLKTLITLRRAEFELLSGGIWMPDSTDAALWQQVCRLRGNGQRVIQALPGQSLSAALEANCDRQLILQNGFWQVSLLAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLE1</accession>
    <name>A0A481QLE1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal RNA large subunit methyltransferase E</fullName>
            <ecNumber evidence="4">2.1.1.166</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">23S rRNA Um2552 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">rRNA (uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">rlmE</name>
        <name evidence="4" type="synonym">ftsJ</name>
        <name evidence="4" type="synonym">rrmJ</name>
        <name evidence="7" type="ORF">C3B55_00364</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O-methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10202"/>
            <dbReference type="Rhea" id="RHEA-COMP:10203"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.166" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00364"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FtsJ" evidence="6">
        <location>
            <begin position="28"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4 5">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="214" mass="24058" checksum="B8658289FA26DFAB" modified="2019-06-05" version="1">MARSKTSHQWLQEHFNDPYVKKAQKNGYRSRASYKLLEIQNKDKLIRPGMSVIDLGAAPGGWSQVVSCLIGERGRLIASDILEMDSIPYVTFVHGDFTQNILLAQIFKAVGNSQVDLIISDMAPNLVGLSTVDMPRVMCLCDLALSLASQVLRPGGDFLVKIFHGEGFDRYYKNINKLFDKVQTRKPAASRNKSREQYLLCRGFLGILASEERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLF5</accession>
    <name>A0A481QLF5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein HflK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hflK</name>
        <name evidence="9" type="ORF">C3B55_00230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">HflC and HflK could encode or regulate a protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">HflC and HflK may interact to form a multimeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the band 7/mec-2 family. HflK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00230"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03404">
        <property type="entry name" value="SPFH_HflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010201">
        <property type="entry name" value="HflK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020980">
        <property type="entry name" value="Membrane_HflK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF2">
        <property type="entry name" value="PTHR43327:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12221">
        <property type="entry name" value="HflK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01933">
        <property type="entry name" value="hflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019745873" description="Protein HflK" evidence="7">
        <location>
            <begin position="22"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="8">
        <location>
            <begin position="103"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="375"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="375"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="410" mass="46026" checksum="4CE6A7BF65EAED2D" modified="2019-06-05" version="1">MALVGVLLVMSLLYQMESSMAWNEPGGNSNNQDPWGGKRRNNDDRKESPDLDEVFRRLQESLNKLFGSRKKRGSGASGRTSKSGGYGLLGLSLVVLIAVWIYSAVYVVDEQEKAVVLRFGKYYDTVGPGLNIYIPPIDTKYMENVTRERAYTKHGQMLTEDENIVEVPLTVQYKISNLQDFVLNVDKPEISLQHATESALRHVVGSTVMDQVLTEGRELMASEIKERLQRFLDTYRTGITVTQVNVQSAAAPHEVQEAFDDVIRAREDEQRSRNQAETYANGVIPEARGQAQRILEDANGYRDEVVSRAKGEADRFTKLVAEYCKAPEVTRERLYLDTMQEVFSNTSKILVTGSQSKQNNLIYLPLDKIIESSRSSTSLPSASSNATTNETNVHAASDLLQQQTRTRESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLG1</accession>
    <name>A0A481QLG1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Penicillin-binding protein 1B</fullName>
            <shortName evidence="13">PBP-1b</shortName>
            <shortName evidence="13">PBP1b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Murein polymerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">mrcB</name>
        <name evidence="19" type="ORF">C3B55_00386</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00386"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009274">
        <property type="term" value="C:peptidoglycan-based cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011813">
        <property type="entry name" value="PBP_1b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028166">
        <property type="entry name" value="UB2H"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14814">
        <property type="entry name" value="UB2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002799">
        <property type="entry name" value="PBP_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02071">
        <property type="entry name" value="PBP_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0133">Cell shape</keyword>
    <keyword evidence="13" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="UB2H" evidence="18">
        <location>
            <begin position="68"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="17">
        <location>
            <begin position="165"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="16">
        <location>
            <begin position="428"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for transglycosylase activity" evidence="14">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate; for transpeptidase activity" evidence="14">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR002799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR002799-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="QAX81738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="774" mass="85661" checksum="CD0236C89010BFA0" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLH5</accession>
    <name>A0A481QLH5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protease TldD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">C3B55_00528</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00528"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="3">
        <location>
            <begin position="38"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="5">
        <location>
            <begin position="127"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="4">
        <location>
            <begin position="244"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="479" mass="51019" checksum="C24428C75A4DE349" modified="2019-06-05" version="1">MRELLPLVSEHLLEPGGVTIENLQTVLGDLSGPDIDAADLYFQGQISESWILEDGIVKEGSFNLDQGVGVRAQSGEKTGFAYSNAITIEALGLAIRTARSISRAGQNGTVQVFSNHGITQLYAYNNPLEVISYTGKVNLLKRIDAATRALDPRIKQVIVSMTGLWEHILVASLDGGLTADIRPLVRFNISVIVEQQGRREHGRHGGGGRTDYRYFLTEDRAMSYAREALRQALINLEAIPAPAGTLPVVLGSGWPGVLLHEAVGHGLEGDFNRKGSSAYSGRIGETVVSKLCTIVDDGTIACCRGSLSIDDEGTPTERTTLIENGVLKGYMQDKLNARLMGVALTGNGRRESYAHLPMPRMTNTYMLSGESDPAEIIASVKRGIYCANLGGGQVDITSGKFVFSTSEAYLLEDGKVTAPIKGAMLIGNGPEVMSKVLMVGNDLSLDSGVGTCSKDGQLLPVGVGQPTVKIDTITVGGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLH9</accession>
    <name>A0A481QLH9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="11">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 16" type="primary">ileS</name>
        <name evidence="16" type="ORF">C3B55_00420</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00420"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="13">
        <location>
            <begin position="27"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="15">
        <location>
            <begin position="691"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="zf-FPG_IleRS" evidence="14">
        <location>
            <begin position="904"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="608"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>L-isoleucyl-5'-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:178002"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="611"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="906"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="909"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="926"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="929"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX81766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="943" mass="106721" checksum="420BF72CD2923ED6" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLI9</accession>
    <name>A0A481QLI9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydroorotase</fullName>
            <ecNumber evidence="2">3.5.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pyrC_1</name>
        <name evidence="2" type="ORF">C3B55_00088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.3" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00088"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01317">
        <property type="entry name" value="DHOase_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="56"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="423" mass="44488" checksum="2D5794ECEE4ADCBE" modified="2019-06-05" version="1">MRRSIIGARVIDPASGLDQITDLYIEAGKIISIGAAPTGFSAIESYQAQGLVAAPGLVDINVALREPGYSHKGNITSETRAAAAGGVTSLCCPPNTKPVIDTSAITKLILDRAREVGNCKVFPIGALSKDLKGEQLAELITLRDAGCIAFGGGLENFCSTRTLRYALEYAATFDLTVILHSQDRDLAEGGLAHEGTVASFLGLPGIPETAETVALTRNLLLVEQSGVRAHFSQLTSARGVALIAQAQACGLPVTADVALYQLILTDEALINFSSLYYVQPPLRTSADRDELRAAVKSGVVSAISSHHQPHERDAKLAPFGATEPGISSVELLLPLALTLVKEGLLDLPTLLARLSTGPAEALRLPAGKLTIGSAADLVLFDPTSFTVAGRRWLSSGTNCPFISHNLPATVRCTLVDGQISYQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLJ0</accession>
    <name>A0A481QLJ0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional repressor NrdR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">nrdR</name>
        <name evidence="11" type="ORF">C3B55_00310</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NrdR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00310"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00440">
        <property type="entry name" value="NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003796">
        <property type="entry name" value="RNR_NrdR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30455">
        <property type="entry name" value="PTHR30455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00244">
        <property type="entry name" value="TIGR00244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ATP-cone" evidence="10">
        <location>
            <begin position="49"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" evidence="8">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="154" mass="17464" checksum="6193AE907059001C" modified="2019-06-05" version="1">MHCSFCGANDTKVINSRLVADGSQVRRRRACLACGERFSTFETAALILPRLIKSDGSCQLFDEGKLRVGMQRALEKRPVSMQQIEAALVRIKHKLRSSGEREIKSMVVGDLVIGELHKLDEVAYIRFASVYLCFQNLNEFREEINRLSSESIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLL2</accession>
    <name>A0A481QLL2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00340"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="134" mass="15549" checksum="B7D4445AC936C819" modified="2019-06-05" version="1">MNAHIKSHNFHCKPSEACYFTNLCKQFNGHMCLTEQCLNLKVHNRGNHYKLIAGHQHTFFTKNLLCCLYRTTKASELSLKTVRLYLQQFTLKNLTSKQVTKVSVNPSKSTNSGPVQNDRKTQERTRYQRHALHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLL3</accession>
    <name>A0A481QLL3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Siroheme synthase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="15">Uroporphyrinogen-III C-methyltransferase</fullName>
                <shortName evidence="15">Urogen III methylase</shortName>
                <ecNumber evidence="15">2.1.1.107</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="15">SUMT</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="15">Uroporphyrinogen III methylase</fullName>
                <shortName evidence="15">UROM</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="15">Precorrin-2 dehydrogenase</fullName>
                <ecNumber evidence="15">1.3.1.76</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="15">Sirohydrochlorin ferrochelatase</fullName>
                <ecNumber evidence="15">4.99.1.4</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="15 21" type="primary">cysG</name>
        <name evidence="21" type="ORF">C3B55_00458</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 H(+) + siroheme = Fe(2+) + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:24360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
            <dbReference type="EC" id="4.99.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) + precorrin-2 + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:32459"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.107"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:15613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="EC" id="1.3.1.76"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="15">Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14 15">Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the C-terminal section; belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="15"/>
    <dbReference type="EC" id="1.3.1.76" evidence="15"/>
    <dbReference type="EC" id="4.99.1.4" evidence="15"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00458"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00211"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00211"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051266">
        <property type="term" value="F:sirohydrochlorin ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01646">
        <property type="entry name" value="Siroheme_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037115">
        <property type="entry name" value="Sirohaem_synt_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012409">
        <property type="entry name" value="Sirohaem_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019478">
        <property type="entry name" value="Sirohaem_synthase_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006367">
        <property type="entry name" value="Sirohaem_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10414">
        <property type="entry name" value="CysG_dimeriser"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036426">
        <property type="entry name" value="Sirohaem_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01470">
        <property type="entry name" value="cysG_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 15 17" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13 15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 15" id="KW-0520">NAD</keyword>
    <keyword evidence="9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 15" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 15 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sirohm_synth_M" evidence="20">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="CysG_dimeriser" evidence="19">
        <location>
            <begin position="151"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="TP_methylase" evidence="18">
        <location>
            <begin position="218"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Uroporphyrinogen-III C-methyltransferase" evidence="15">
        <location>
            <begin position="216"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="15">
        <location>
            <begin position="301"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="15">
        <location>
            <begin position="331"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15 16">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15 16">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="43"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="15">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR036426-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF10414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="QAX81798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="457" mass="49745" checksum="F94C038EFF301A72" modified="2019-06-05" version="1">MEFLPLFHNLRGSRVLVVGGGKISLRKSRLLAAAGALLRVVAPRIEDHLRELVLGSGGELILRGYQESDLDGHNLIIVATDNAPLNAQVSHDAQCRCLPVNVVDAPALCSVIFPAIVDRSPLVIAVSSSGVAPVLARLIRAKLETWIPSTYGQLARLAARFRTQVKGLYPDAQQRLAFWEEVFQGPIADRQLAGQSDEAERLLIEKVHGKSLHAQGEVYLVGAGPGCPDLLTFRAMRLMQQADVVLYDRLVAPTILELCRRDAERIYVGKCRADHTVPQDQINQQLVELAKQGKRVLRLKGGDPFIFGRGGEEIAELAIHGIPFQVVPGITAASGCAAYAGIPLTHRDYAQSVRFITGHLKDGTSDLPWGELIGLSQTLVFYMGLISLPIICEQLIKHGRAADTPAALIQQGSTSNQRVFTGTLADLPCIVAKHEVHAPTLVIVGEVVVLREKLKWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLM0</accession>
    <name>A0A481QLM0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTP cyclohydrolase-2</fullName>
            <ecNumber evidence="5">3.5.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP cyclohydrolase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">ribA</name>
        <name evidence="7" type="ORF">C3B55_00315</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 3 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00315"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00179">
        <property type="entry name" value="RibA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00505">
        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydro2" evidence="6">
        <location>
            <begin position="8"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="49"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="205" mass="22462" checksum="77A5A239F6ED0B2D" modified="2019-06-05" version="1">MPTVFVAASKLPTTFATFTMNGFLEQATGREHVVLSLGSIADGAPVLSRVHSECLTGDALFSQRCDCGSQLEAAMRAIASECRGVLLYLRQEGRGIGLMNKIRAYKLQDGGADTVEANELLGFAADQRNYAICLPMLEYLGVKSIRLMTNNPRKVKALSEMGITVTERVPLQTGQNQYNKLYLATKARKLDHMMGNDFHGEVDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLM4</accession>
    <name>A0A481QLM4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">C3B55_00547</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00547"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="259" mass="27525" checksum="E0FECC2FD910438E" modified="2019-06-05" version="1">MGNFSFVIAGLIVGFIVGMTGVGGGSLMTPILLWLGINPATAVGTDLLYAAITKSGGALVHSKNKNIDWTITGWLTLGSIPAVLLTLWCFACLHTDTSAINVVIKQALGLVLLLTAMAILFKKSLLIFAKRYANDDYYMSQRNLNALTSVTGAILGIMVALTSIGAGALGTVVLFILYPFLATRRLVGTEIAHAVPLTLVAGLGHASMGNMNWHLLSFLLIGSLPGIYFGSHMTEKVPDEVLRPCLAIMLMVIGYKLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLN5</accession>
    <name>A0A481QLN5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription termination/antitermination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nusA</name>
        <name evidence="9" type="ORF">C3B55_00373</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Participates in both transcription termination and antitermination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00373"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_B">
        <property type="entry name" value="NusA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036555">
        <property type="entry name" value="NusA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013735">
        <property type="entry name" value="TF_NusA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010214">
        <property type="entry name" value="Tscrpt_termin_fac_NusA_C_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010213">
        <property type="entry name" value="Tscrpt_termination_fac_NusA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08529">
        <property type="entry name" value="NusA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="SSF47794"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69705">
        <property type="entry name" value="SSF69705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01953">
        <property type="entry name" value="NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01954">
        <property type="entry name" value="nusA_Cterm_rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="5 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 7" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="137"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="493" mass="54661" checksum="4AFEAD712BDAF1DD" modified="2019-06-05" version="1">MSKEVLLVVESVSNEKGVPAIVIFEALELALTTATKKRFEDNVDLRVEINRHTGAYETFRRWIVVEEADFNNAAIEIWPNKVVKTHPGAKVGDVVEEKIESIEFGRIAAQTAKQVIVQKVREAERAQVVNAYRERLGEIISGIVKKVTRDNVIVDLGNNAEALLAREDIISRETIRVGLRLRALLKEIRIESRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEIMAASRDPGSRAKIAVRSKDKRIDPQGACIGMRGSRVQAVSSELDGERMDIVLWDDNSAQFVINAMSPAEVASIIVDEDTHAMDIAVSADNLAQAIGRGGQNVRLASQLTGWSLNVMTELDIQAKQQAETSDILCNFINELEVDEDLAQVLVDEGFTSLEEIAYVPLEELLDIDGIDEDTVNELRVRAKDRLLTKAIATEEKLADTDLAEDLLTLDGMDKSLAMELAVRGIITREDLAEQSIDDLLNIDGIDNNRAGKLIMAARAHWFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLN9</accession>
    <name>A0A481QLN9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00492"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="53" mass="6237" checksum="A5C84A601B2917B1" modified="2019-06-05" version="1">MSEANLDATKSIALFKFKQFQDELYSFFYRFISFQNTLISTVYPLLITELESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLP1</accession>
    <name>A0A481QLP1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00163"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="45" mass="5044" checksum="4517103E13E97998" modified="2019-06-05" version="1">MQSNICLNKQISIGQNYKVILPDFLVMPVSVLCLISHPVFSRAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLP6</accession>
    <name>A0A481QLP6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00173"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="37" mass="4371" checksum="A1C7C961539467E8" modified="2019-06-05" version="1">MDHIKRIQLMLCKIGIKFNGLISKRSNYLDNLSIEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLQ4</accession>
    <name>A0A481QLQ4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydropteroate synthase</fullName>
            <shortName evidence="1">DHPS</shortName>
            <ecNumber evidence="1">2.5.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dihydropteroate pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">folP</name>
        <name evidence="3" type="ORF">C3B55_00366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DHPS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00366"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00156"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="PTHR20941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="2">
        <location>
            <begin position="22"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="283" mass="30389" checksum="C3064D23719F81AA" modified="2019-06-05" version="1">MTSVLSSTRLPCGNRVLDLDHTHVMGILNVTPDSFFDSSRFSQLDAALRHAEAMVAAGTALIDVGGESTRPGARVVSSLEELERVSSIVELIAHELDVIISVDTSNPAVMREAASLGAGLINDVRSLQRDGAMDAAVATGLPVCLMHMLGEPGNMQDNPHYYDLIGEVSGFLAERMAQCINAGISPEKIILDPGFGFAKTRQHNFSLLKHMESLCYLGRPLLVGVSRKSMTGFTLNHPISERLYGSLALAALAVIRGARILRVHDVAETVDIVRMLTAVESAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLU3</accession>
    <name>A0A481QLU3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">folK</name>
        <name evidence="14" type="ORF">C3B55_00381</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of pyrophosphate from adenosine triphosphate (ATP) to 6-hydroxymethyl-7,8-dihydropterin, an enzymatic step in folate biosynthesis pathway.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HPPK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00381"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="SSF55083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPPK" evidence="13">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="161" mass="18011" checksum="2A8C4EE3F986D229" modified="2019-06-05" version="1">MERIYIGMGSNIAAPNQQLCNAIEALALVPNTTVACVSTFYQSDSLLPGQPRYTNAVAAIDSNLAPLDLLDALQAIENNQGRERLERWGPRTLDLDILLFGEHLIDKPRLKVPHYQMQLRAFVIYPLAELAPADLQLPNGPILRVLLTTCPFMNLERIRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLU4</accession>
    <name>A0A481QLU4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor IF-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 5" type="primary">infA</name>
        <name evidence="5" type="ORF">C3B55_00453</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IF-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_002553999.1">
        <property type="nucleotide sequence ID" value="NZ_CP026512.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A481QLU4"/>
    <dbReference type="GeneID" id="58767738"/>
    <dbReference type="GeneID" id="61931168"/>
    <dbReference type="GeneID" id="64443124"/>
    <dbReference type="GeneID" id="67381632"/>
    <dbReference type="KEGG" id="pade:C3B55_00453"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04451">
        <property type="entry name" value="S1_IF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00075">
        <property type="entry name" value="IF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="72" mass="8303" checksum="97E5EEFD8F3886DB" modified="2019-06-05" version="1">MSKEDSFEMEGTVVDTLPNTMFRVELENGHVVTAHISGKMRKNYIRILTGDKVRVELTPYDLSKGRITYRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLU7</accession>
    <name>A0A481QLU7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional purine biosynthesis protein PurH</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">Phosphoribosylaminoimidazolecarboxamide formyltransferase</fullName>
                <ecNumber evidence="8">2.1.2.3</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">AICAR transformylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="8">IMP cyclohydrolase</fullName>
                <ecNumber evidence="8">3.5.4.10</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">ATIC</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="8">IMP synthase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="8">Inosinicase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">purH</name>
        <name evidence="10" type="ORF">C3B55_00248</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The IMP cyclohydrolase activity resides in the N-terminal region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PurH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.3" evidence="8"/>
    <dbReference type="EC" id="3.5.4.10" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00248"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="PTHR11692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="9">
        <location>
            <begin position="6"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="535" mass="58096" checksum="3FBCE2505DBE0EC6" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLW6</accession>
    <name>A0A481QLW6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7 9">50S ribosomal protein L16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">rplP</name>
        <name evidence="11" type="ORF">C3B55_00284</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 9">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00284"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01342">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_L16_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="PTHR12220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00586">
        <property type="entry name" value="RIBOSOMAL_L16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2 7 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L16" evidence="10">
        <location>
            <begin position="4"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="137" mass="15443" checksum="7BD251779FF68985" modified="2019-06-05" version="1">MLQPKRTKFRKQMTGHNRGLAQCGSKVNFGEFALKSIARGRLTARQIESARRALTRHVKRGGKIWIRVFPDKPISKKPLEVRMGKGKGNVEYWVAQIQPGKVLYEIEGIAEELAREAFALAAAKLPLATVFIKRTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLW7</accession>
    <name>A0A481QLW7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00491"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="30" mass="3440" checksum="613BCCF358057DA7" modified="2019-06-05" version="1">MEHNNKMLTIGERVENIFFGAGANNCTQYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLX0</accession>
    <name>A0A481QLX0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00699</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00699"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="34" mass="3805" checksum="1F898EDBF9E0968D" modified="2019-06-05" version="1">MLDQLLVAEQVNAINWCDGALHLLNQSALPSWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-02-10" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLY3</accession>
    <name>A0A481QLY3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein TusB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tusB</name>
        <name evidence="1" type="ORF">C3B55_00460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00460"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007215">
        <property type="entry name" value="Sulphur_relay_TusB/DsrH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04077">
        <property type="entry name" value="DsrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03011">
        <property type="entry name" value="sulf_tusB_dsrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="92" mass="10222" checksum="33C0814F613DD640" modified="2019-06-05" version="1">MSTLHVVSYSPFTNSCLASCLRVCSAENAILLCGDGTYGLYNATLQTHGVKVFVLAEDIQARNLLLPDWAYDVDYPGFVQLSIDYDKVNIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLZ7</accession>
    <name>A0A481QLZ7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sulfite reductase [ferredoxin]</fullName>
            <ecNumber evidence="7">1.8.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sir</name>
        <name evidence="7" type="ORF">C3B55_00484</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.7.1" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00484"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050311">
        <property type="term" value="F:sulfite reductase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="PTHR11493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIR_SIR_ferr" evidence="6">
        <location>
            <begin position="53"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="NIR_SIR" evidence="5">
        <location>
            <begin position="119"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="NIR_SIR_ferr" evidence="6">
        <location>
            <begin position="330"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="NIR_SIR" evidence="5">
        <location>
            <begin position="452"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="552" mass="62599" checksum="D84073CA3A59A2A9" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM20</accession>
    <name>A0A481QM20_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA threonylcarbamoyladenosine biosynthesis protein TsaB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tsaB</name>
        <name evidence="5" type="ORF">C3B55_00497</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KAE1 / TsaD family. TsaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00497"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022496">
        <property type="entry name" value="T6A_TsaB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF11">
        <property type="entry name" value="PTHR11735:SF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03725">
        <property type="entry name" value="T6A_YeaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TsaD" evidence="4">
        <location>
            <begin position="30"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="228" mass="24704" checksum="1648D6DFA088CFB2" modified="2019-06-05" version="1">MSTLLAIDTATEACSVALLQNGKVTSHYEVVPRLHSQKLLPMIKKLLEETGTKLSTVDAIAFGRGPGAFTGVRLTIGVVQGLAFALKCPVLPVSNLAVLAQRALREYGATQVAAAIDARMDEVYWGCYREMAGEMCLVGAEVVQSPEFLVLSDNTTSDWFGAGTGWSYSKRILVKICGHDDAMLPHAIDLLTLARFAFKRGEEIPADQAAPTYLRNKVTQAKPSYEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM29</accession>
    <name>A0A481QM29_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotide-binding protein C3B55_00526</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Displays ATPase and GTPase activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RapZ-like family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00526"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00636">
        <property type="entry name" value="RapZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005337">
        <property type="entry name" value="RapZ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30448">
        <property type="entry name" value="PTHR30448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03668">
        <property type="entry name" value="ATP_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005052">
        <property type="entry name" value="P-loopkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="60"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="285" mass="32393" checksum="A6B42B00F618330E" modified="2019-06-05" version="1">MRLIIVSGRSGSGKSTALNVLEDNGFYCIDNLPAGLLPELVERAMINTELTQLLIAVSIDARNLPGHLERFPQLLEEVRAKHIHCDVLYLDADEETLLKRFSETRRRHPLGSPHRSLAEAIEDETVLLGPIIDLAGLKINTTNLNLYQLYNAMKLHLLHQPEPSTEFLIESFGFKRGIPVDANLVFDVRCLPNPYWKPELRDQSGLDQPVAKYLAEQLDVEEMFQDISKYLLKWLPRFTTSNRAYVTIAIGCTGGYHRSVYLAERLGHVLQQSLKNVQVRHRDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM45</accession>
    <name>A0A481QM45_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxine 5'-phosphate synthase</fullName>
            <shortName evidence="2">PNP synthase</shortName>
            <ecNumber evidence="2">2.6.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">pdxJ</name>
        <name evidence="3" type="ORF">C3B55_00555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = H(+) + 2 H2O + phosphate + pyridoxine 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15265"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="EC" id="2.6.99.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.99.2" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00555"/>
    <dbReference type="UniPathway" id="UPA00244">
        <property type="reaction ID" value="UER00313"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033856">
        <property type="term" value="F:pyridoxine 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00003">
        <property type="entry name" value="PNPsynthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00279">
        <property type="entry name" value="PdxJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004569">
        <property type="entry name" value="PyrdxlP_synth_PdxJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036130">
        <property type="entry name" value="Pyridoxine-5'_phos_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30456">
        <property type="entry name" value="PTHR30456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03740">
        <property type="entry name" value="PdxJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63892">
        <property type="entry name" value="SSF63892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00559">
        <property type="entry name" value="pdxJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="1-deoxy-D-xylulose 5-phosphate binding" evidence="2">
        <location>
            <begin position="14"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="region of interest" description="3-amino-2-oxopropyl phosphate binding" evidence="2">
        <location>
            <begin position="218"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="256" mass="28120" checksum="EEA806A57F17B016" modified="2019-06-05" version="1">MATSNRILLGVNIDHVATIRQARGTRYPDPIKAALDAEEAGADGITVHLREDRRHIQERDVLLLKDVLQTRMNFEMGVTEEMMVFAERIRPAHICLVPETRKELTSEGGLDVVNNEARIKAAVARLLRICSEVSLFIDADECQIVAASRVGAPAIELHTGRYANVTAPIKVLDELQRLIAGVNCGLNQGLIVNAGHGLNYQNVEDIAMINGINELNIGHALVAHALSVGLKNAIVEMKTLILACRLRHAITYVQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM57</accession>
    <name>A0A481QM57_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribonuclease 3</fullName>
            <ecNumber evidence="7">3.1.26.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ribonuclease III</fullName>
            <shortName evidence="7">RNase III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">rnc</name>
        <name evidence="11" type="ORF">C3B55_00557</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribonuclease III family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00557"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="PTHR11207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02191">
        <property type="entry name" value="RNaseIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="RNase III" evidence="10">
        <location>
            <begin position="5"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="9">
        <location>
            <begin position="154"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="226" mass="25246" checksum="CABAACF24EA10175" modified="2019-06-05" version="1">MTVLLSCLERQLGYVFKDQELMVLALTHRSFTGRNNERLEFLGDAILNFIAGQALFERFPQAHEGQLSRLRARLVKGETLAMLARSFGLGKYLRLGSGELKNGGCCRASILADTIEAIIGAIYLDAGIEAVKKHVTAWLHFELESLTLIDTHKDPKTRLQEFLQARRCELPCYTLVNIQGKPHCRTFFVECEIILLNKKSLGQGMSRRIAEQLAATAALIALGVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM62</accession>
    <name>A0A481QM62_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="5 8">3.4.21.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Endopeptidase Clp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 11" type="primary">clpP</name>
        <name evidence="11" type="ORF">C3B55_00605</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 9">Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 10">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="5 8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00605"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00493">
        <property type="entry name" value="clpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0720">Serine protease</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="213" mass="24037" checksum="1E52672D71F96471" modified="2019-06-05" version="1">MFRNFYIQQNSDIQATSSLVPMVVEQSARGERAYDIYSRLLKERVIFLVGPVEDYMANLICAQLLFLEAENPDKDIHLYINSPGGSVTAGMSIYDTMQFIKPNVSTTCIGQACSMGAFLLTAGSEGKRFCLPNSRVMIHQPLGGFHGQASDIEIHAKEILFIRERLNTLMAKHSKHTLEEIEHDTNRDNFMSAEAARKYGLIDEVIDKRPFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM70</accession>
    <name>A0A481QM70_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">grxD</name>
        <name evidence="10" type="ORF">C3B55_00618</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00618"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097573">
        <property type="term" value="F:glutathione oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014434">
        <property type="entry name" value="Monothiol_GRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="PTHR10293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005894">
        <property type="entry name" value="Monothiol_GRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00365">
        <property type="entry name" value="TIGR00365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 8" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="9">
        <location>
            <begin position="16"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glutathione binding" evidence="7">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="112" mass="12085" checksum="421DEDD7B2439790" modified="2019-06-05" version="1">MNIIDTIKEQITNNTILLYMKGAPNAPQCGFSARASRTIMACGEKFAYVDILQNPEIRANLPKYANWPTFPQLWVAGKLVGGSDIIADMAADGLLQALIKEAATNASAKTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM81</accession>
    <name>A0A481QM81_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA topoisomerase 1</fullName>
            <ecNumber evidence="10">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">topA</name>
        <name evidence="12" type="ORF">C3B55_00714</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00714"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03363">
        <property type="entry name" value="TOPRIM_TopoIA_TopoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005733">
        <property type="entry name" value="TopoI_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013263">
        <property type="entry name" value="TopoI_Znr_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034149">
        <property type="entry name" value="TOPRIM_TopoI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42785">
        <property type="entry name" value="PTHR42785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08272">
        <property type="entry name" value="Topo_Zn_Ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="topA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TOPOISOMERASE_I_PROK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="11">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="10">
        <location>
            <begin position="199"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
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            <position position="179"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="873" mass="98739" checksum="523A165952C8C5FD" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM82</accession>
    <name>A0A481QM82_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00587</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00587"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="53" mass="6131" checksum="76D8C5D5C82DB01A" modified="2019-06-05" version="1">MVIASKWHMVVKPNNNFFSKTLHSKYQLLIPVPTNTSEHTSTVYVVFAAYFNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM92</accession>
    <name>A0A481QM92_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="9">HTPA synthase</shortName>
            <ecNumber evidence="4 9">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">dapA</name>
        <name evidence="11" type="ORF">C3B55_00725</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="4 9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00725"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="292" mass="31325" checksum="82B6327646AC64B2" modified="2019-06-05" version="1">MIAGSMVALVTPMNARGHLDWDSLGKLVDLHLQEGTNAIVVVGTTGESVTLDAKEHIQVINFVVTRVAGRIAVIAGTGANSTREAIELTQNAKKAGANACLLVTPYYNRPTQEGLYQHFRVIAEEVDIPQILYNVPGRTACDMKVETVIRLSTVPNIIGIKEATGDLQRAKGILAGVSRDFLVYSGDDATAVELILLGGKGNISVTANVVPRAMSDMCAAAIAGDAAIARAIHERLMPLNKTLFIESNPIPVKWALFEMGLIPEGIRLPLTRFSEACHEPLRQALRQSGVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM99</accession>
    <name>A0A481QM99_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00650"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="40" mass="4719" checksum="5F4F6E707A416F6A" modified="2019-06-05" version="1">MHNVSNLCILQIAEYYKRVILQEIIDLIKIWTKIPTLGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMA6</accession>
    <name>A0A481QMA6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="4 14">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">Bacitracin resistance protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 14">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">uppP</name>
        <name evidence="15" type="ORF">C3B55_00481</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the UppP family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="4 14"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00481"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="PTHR30622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00753">
        <property type="entry name" value="undec_PP_bacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="6 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0133">Cell shape</keyword>
    <keyword evidence="14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX81819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="276" mass="30718" checksum="1F82C2A98566155D" modified="2019-06-05" version="1">MNFWVIIQALILGVVEGLTEFLPISSTGHQIIIAELIGFEDKHFKLFNIIIQLGAILAVIWEFRRKILNVILSLPNQRNSQRLTVNLIVALLPAVALGMIVIDLMKHDPFSPIIVAITLVVGGMIMLWVESRQHLVHAKTVYDITWKDALKVGVAQCFSIIPGTSRSGATIICGLLSGLSRTAATEFSFFLAIPTMIGAAVYSCYKYRDLCQQDALPLFAIGFVASFIFAMMTVKALLKFIASHSYAAFAWYRIAFGLIILTTWHFGWINWTTVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMB1</accession>
    <name>A0A481QMB1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c-type biogenesis protein CcmE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cytochrome c maturation protein E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Heme chaperone CcmE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">ccmE</name>
        <name evidence="4" type="synonym">cycJ</name>
        <name evidence="8" type="ORF">C3B55_00673</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CcmE/CycJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00673"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017003">
        <property type="term" value="P:protein-heme linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01959">
        <property type="entry name" value="CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004329">
        <property type="entry name" value="CcmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036127">
        <property type="entry name" value="CcmE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34128">
        <property type="entry name" value="PTHR34128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03100">
        <property type="entry name" value="CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82093">
        <property type="entry name" value="SSF82093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 4" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="1 4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="4">
        <location>
            <begin position="1"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="4">
        <location>
            <begin position="30"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="4 5">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="4 5">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604329-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="153" mass="16666" checksum="DF12CD6BFF052055" modified="2019-06-05" version="1">MNPLRRKRLLIILANLVGVSITVGLMMSALRENINLFYTPTQIANGDAPLGKRIRAGGIVEKGSLKRSSNSLDVIFVVTDFNKAVTITYRGVLPDLFREGQGVVALGKLNTNGVVIADEVLAKHDEKYMPPEINKNLKDSGPIRVSPGQVNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMC4</accession>
    <name>A0A481QMC4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Carboxynorspermidine/carboxyspermidine decarboxylase</fullName>
            <shortName evidence="10">CANS DC/CAS DC</shortName>
            <shortName evidence="10">CANSDC/CASDC</shortName>
            <ecNumber evidence="6 10">4.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nspC</name>
        <name evidence="13" type="ORF">C3B55_00503</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the decarboxylation of carboxynorspermidine and carboxyspermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>carboxynorspermidine + H(+) = CO2 + norspermidine</text>
            <dbReference type="Rhea" id="RHEA:34099"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57920"/>
            <dbReference type="ChEBI" id="CHEBI:65070"/>
            <dbReference type="EC" id="4.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>carboxyspermidine + H(+) = CO2 + spermidine</text>
            <dbReference type="Rhea" id="RHEA:34095"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:65072"/>
            <dbReference type="EC" id="4.1.1.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the Orn/Lys/Arg decarboxylase class-II family. NspC subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.96" evidence="6 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00503"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045312">
        <property type="term" value="P:nor-spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06829">
        <property type="entry name" value="PLPDE_III_CANSDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005730">
        <property type="entry name" value="Nsp_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43727:SF1">
        <property type="entry name" value="PTHR43727:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038941">
        <property type="entry name" value="NspC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0745">Spermidine biosynthesis</keyword>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="12">
        <location>
            <begin position="174"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038941-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="365" mass="41082" checksum="B559EFD09DD059F2" modified="2019-06-05" version="1">MIKTPYYLIDKQKLLVNMQKIAYLREQSGAKVLLALKCFATWSLFDLMQQYMDGTTSSSLYELKLGHQKFKGEAHAYSVAWADDEIEESLDNCDKIIFNSINQLHRFTERSEGKAIGLRVNPQISSSSYLLSDSARPFSRLGEWDPVKIESVIEQVSGLMFHNNCENSDFSLFDKMLSAIEKCFSKLLYQVQWVSLGGGIHFTGKDYALDTFCARLKAFSKKYSVQVYLEPGEAAITNSASLEVTVLDTLYNNKNLAVVDSSIEAHLPDLLIYRLNAVLAQSKGKYTYMVCGKSCLAGDIFGEYQFNCPLAIGDRLSFIDTAGYTIVKKNWFNGLKMPSIVVKQLDGTVELVCEFSYDDYLSSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMC9</accession>
    <name>A0A481QMC9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide chain release factor 1</fullName>
            <shortName evidence="5">RF-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">prfA</name>
        <name evidence="7" type="ORF">C3B55_00408</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Methylated by PrmC. Methylation increases the termination efficiency of RF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00408"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0488">Methylation</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="6">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="5">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="360" mass="40426" checksum="DDF61984FE7E9276" modified="2019-06-05" version="1">MKASLLNKLEVLQDRFEELTALLSDSAVISDQTKFRAYSKKYTEVKPIVETYKSLMKVLTRLDGAQELLKDCDPDIREMAMEEVRETKEKLTTLEGYLKLMLLPKDPDDWRNVFLEIRAGTGGDEAALFSGDLFRMYSRYAERCGWSIKILSENEGEQGGYKEVIARVEGDNVYSKLKFESGVHRVQRVPATESQGRIHTSTCTVAVLPELDEQEAIKINQADLRIDTYRSSGAGGQHVNMTDSAIRITHLPSGIVVGCQEERSQHKNRVRAMSWLLAKLNDQQVSAATNAIASERKLLVGSGDRSERIRTYHFARGRVTDHRINLTIYALDEILAGGVDAVIEPLLAEYQANQLVAQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMD0</accession>
    <name>A0A481QMD0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclohexadienyl dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00694</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00694"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="309" mass="33934" checksum="C14FE87A7F1AF3DE" modified="2019-06-05" version="1">MVDFNVLQPSAPMIGRLVVIGLGLIGGSFAKGLRKSGLCDEVIGMDLDQQSRKLAVKLGVVDRCEVDLALACNGADVIQLAVPVLAMEKLLGLLANIDLGHAILTDVGSVKSNVVRVARFAFNDMLARFVPGHPIVGSERGGIEASNEQLFRHYKVILTPLEQTDPAALAVVDRLWCELGADVEYMQVERHDKVLALTSHLPHLLAFCFVDSLAKRNEIFDMFHYAAGGLCDFTRIASSDPVMWRDIFLANREAVLCALDTFLGDLDSFRSAVDTGDGRQLLSVCTRARMAREYFSNILVHRAYIENDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMF4</accession>
    <name>A0A481QMF4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rluC</name>
        <name evidence="5" type="ORF">C3B55_00883</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00883"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="321" mass="36172" checksum="C9FBFB03C09DB3F6" modified="2019-06-05" version="1">MMTAAPQTPNVQHIEVLPEYAGQRIDNFLLSRLKGVPKTLIYRILRQGAVRVNSGRIKPKYKLYTGDIVRVPPVRMSERNESVSLSYSLLQRLEASIVFEDNKLIALNKPCGIAVHGGSGLNFGVIEAFRQLRPDATELELVHRLDRDTSGLLMIAKKRSMLRYLHNALHSDIVDKRYMALVRGNWAGSIKSVCVPLCKSSLRSGERIVTVGENGKEAKTMFKVLHRFGNFATMVEVKPMTGRTHQIRVHSLYAGHCIAGDTKYGEEYFSKKIYDLGGKRLFLHAHMLTIPLPNGGELKLQAPVDEMWAKTVERLNLEPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMF7</accession>
    <name>A0A481QMF7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="10">Glu-ADT subunit A</shortName>
            <ecNumber evidence="4 10">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">gatA</name>
        <name evidence="13" type="ORF">C3B55_00535</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="4 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00535"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="10">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="483" mass="52559" checksum="14C10F7DB0B683E4" modified="2019-06-05" version="1">MHNMTLAEIARGLADKKFSSEELIKTLLIRITELDHKVNSFISITEELAIDQARAADTRRANGENSALLGVPIAHKDLFCTKGIRTSCGSKMLDNFKAPYDATVVLKLAAAGAVTLGKTNMDEFAMGSASESSYYGAVKNPWNLQYVPGGSSGGSAAAVAARFLPATTATDTGGSIRQPAAFTNLTGLKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCATLLQGMAGFDKQDSTSIDEPMPDNNVSLNVSLKGLRIGVPKEYLGADLDPRIAELVHNSIKELEKLGAVIKDINLPSSQYAIIAYYVIALAEASSNLSRFDGVRFGYRCENPKDLEDLYKRSRSEGFGAEVQRRIMVGIYVLSAGYYNAYYIKAQKIRRMIKNDFMAAFKEVDVIIGPTTPNLAWKIGAKISNPMTEYLEDLYTINANLTGLPGLSMPAGFIDGLPVGVQLLAPYFQESRLLNIAHQYQQNTDWHTRTPTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMH5</accession>
    <name>A0A481QMH5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inner membrane-spanning protein YciB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">yciB</name>
        <name evidence="2" type="ORF">C3B55_00908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in cell envelope biogenesis, maintenance of cell envelope integrity and membrane homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00908"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00189">
        <property type="entry name" value="YciB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006008">
        <property type="entry name" value="YciB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36917">
        <property type="entry name" value="PTHR36917"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04279">
        <property type="entry name" value="IspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="198" mass="22626" checksum="D9FBA441AADE139D" modified="2019-06-05" version="1">MKKLIDFIPLLLFFIVTKLDPIVIEISRHKLLFGGIYSATAVLIISSIVIYSAIFIIQRKLEKSQWLSLFACLVFGGLTLAFHNETFLKWKAPVINWLLAMVFMVNYFIGDHLLIKKIMGHTLTLPESIWTRLSVAWIIFFIFCGAANLFVAFTFQAYWVDFKIFGSLGMTVLFLVGQGIYLSRHLHSAVLNTPKTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMH8</accession>
    <name>A0A481QMH8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00690"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="291" mass="34060" checksum="3A189C9C6852C6A5" modified="2019-06-05" version="1">MIQNTVHSKLSILIVLYKKHYKNSVAITTLLRNAVCFNKKGISVEFFIWNNTPLCSPILSKQCLTWFEGNNNGLPYIYNRIANIAFMQGATHFMISDDDTDYSSEKYTDAVVAAMIFESRLASNLFGVMLPKMYSKEKLVSPGLRFWFFCRLATRIDSGIVTSANKLAINSGIIFNKLCYKTISPMFDERLQFYATDTAFFINYESYYAHFYVLDVALQHDLSEYTSNSVELAIFRFKEMIRGFRVIFEHKSYLFRYLMEAYLALYSVKKSFLYKKIVFFRLYIFSFKEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMJ3</accession>
    <name>A0A481QMJ3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="10">2.4.1.227</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">murG</name>
        <name evidence="13" type="ORF">C3B55_00576</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-glucosamine = di-trans-octa-cis-undecaprenyl diphospho-[N-acetyl-alpha-D-glucosaminyl-(1-&gt;4)]-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:31227"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="ChEBI" id="CHEBI:61388"/>
            <dbReference type="EC" id="2.4.1.227"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 28 family. MurG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.227" evidence="10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00576"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051991">
        <property type="term" value="F:UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050511">
        <property type="term" value="F:undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00033">
        <property type="entry name" value="MurG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006009">
        <property type="entry name" value="GlcNAc_MurG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01133">
        <property type="entry name" value="murG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="9 10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 10 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="11">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_tran_28_C" evidence="12">
        <location>
            <begin position="178"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-N-acetyl-alpha-D-glucosamine binding" evidence="10">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="352" mass="37735" checksum="7DF9A0250B25EAA7" modified="2019-06-05" version="1">MLIMAGGTGGHVFPAMACAREFQNRGYIVHWLGAPHGIENAVVRSAGLPLHLINVTGLRGKSKWSLLQAPFVLLKAMWQALNIIHELKPVCVLGFGGYVTGPGGVAAKLTGVPVIVHEQNTVAGTANRMLIPLASRICEAFPATFSASDKLRTTGNPVRTELFIAIDRQVLNGRKLRLLILGGSLGAEPLNKLLPKSLALLPLALRPEVFHQAGSQYSEVTITRYREAGIQANVQSFIQDMAHAYRWADLVVCRAGALTISELAAAGLPSLLVPLPQAINNHQTRNAEYLVSEGAAFLLQQRTTDCADLAACLTGVFMQPERLNSMACNASRLAKPNATSTVVDVCLEVAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMJ4</accession>
    <name>A0A481QMJ4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-binding protein Uup</fullName>
            <ecNumber evidence="5">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">uup</name>
        <name evidence="8" type="ORF">C3B55_00718</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probably plays a role in ribosome assembly or function. May be involved in resolution of branched DNA intermediates that result from template switching in postreplication gaps. Binds DNA and has ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Associates with ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ABC transporter superfamily. ABCF family. Uup subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00718"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00848">
        <property type="entry name" value="Uup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032524">
        <property type="entry name" value="ABC_tran_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043686">
        <property type="entry name" value="Uup"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16326">
        <property type="entry name" value="ABC_tran_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="4"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="317"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <begin position="350"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="639" mass="71323" checksum="30020AD2797CAE84" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMN0</accession>
    <name>A0A481QMN0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 16">Aconitate hydratase B</fullName>
            <ecNumber evidence="5 16">4.2.1.3</ecNumber>
            <ecNumber evidence="6 16">4.2.1.99</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">2-methylisocitrate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">acnB</name>
        <name evidence="22" type="ORF">C3B55_00620</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis-aconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:17941"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="ChEBI" id="CHEBI:57872"/>
            <dbReference type="EC" id="4.2.1.99"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="17">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Organic acid metabolism; propanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="5 16"/>
    <dbReference type="EC" id="4.2.1.99" evidence="6 16"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00620"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00718"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00946"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047456">
        <property type="term" value="F:2-methylisocitrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047780">
        <property type="term" value="F:citrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01576">
        <property type="entry name" value="AcnB_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004406">
        <property type="entry name" value="Aconitase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015933">
        <property type="entry name" value="Aconitase_B_HEAT-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036288">
        <property type="entry name" value="Aconitase_B_HEAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015929">
        <property type="entry name" value="Aconitase_B_swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06434">
        <property type="entry name" value="Aconitase_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11791">
        <property type="entry name" value="Aconitase_B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036687">
        <property type="entry name" value="AcnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74778">
        <property type="entry name" value="SSF74778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00117">
        <property type="entry name" value="acnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12 17" id="KW-0408">Iron</keyword>
    <keyword evidence="13 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14 16 22" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9 16" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase_B_N" evidence="21">
        <location>
            <begin position="4"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase_2_N" evidence="20">
        <location>
            <begin position="169"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase" evidence="19">
        <location>
            <begin position="475"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="18">
        <location>
            <begin position="244"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="18">
        <location>
            <begin position="417"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="713"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="772"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="775"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="794"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="799"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR036687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR036687-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR036687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF06434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF11791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="QAX81949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="869" mass="94869" checksum="82EDA66E7F4F602E" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMN3</accession>
    <name>A0A481QMN3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName evidence="4">PRAI</shortName>
            <ecNumber evidence="2 4">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">trpF</name>
        <name evidence="6" type="ORF">C3B55_00802</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="2 4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00802"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="PTHR42894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PRAI" evidence="5">
        <location>
            <begin position="11"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="219" mass="23661" checksum="943D63DC76A201E1" modified="2019-06-05" version="1">MSAVMSVVRSKICGITRIEDALAAVDAGADAIGFVFYAKSPRAINVFQALEIVAALPPFVTTVGLFVNPSCYELNKTLDVVQLDMLQFHGDESSAECESYKRPYIKALRVKVGDDIATACNAYTGARGILLDAYVKSVPGGTGEVFDWKLIPKGLNKPIILAGGLNHANVSVAIKQVRPYAVDVSSGVEQDKGIKNHHKIHAFMQAVHKSSRINMMINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMN9</accession>
    <name>A0A481QMN9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00632</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00632"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="44" mass="5154" checksum="AE3CCBAAF1F61C38" modified="2019-06-05" version="1">MLQNYITVVNIYFLNFFFSINEKKKLIIEPHNPALALAIICRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMP1</accession>
    <name>A0A481QMP1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative 3-phenylpropionic acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hcaT</name>
        <name evidence="7" type="ORF">C3B55_00815</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00815"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026032">
        <property type="entry name" value="HcaT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004925">
        <property type="entry name" value="HcaT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="141"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="381" mass="42153" checksum="51374DF262FAAF10" modified="2019-06-05" version="1">MIALPYWRLSSFYLFYFALLGATAPFLALYFDYLGFSSARIGELLAIPMLMRCVAPSLWGWLSDYTGWHLAIVRFGAVCTLASFSLIFINKTYAWLAMVMALHAFFWHAVLPQFEVITLAHLQMQTACYSQIRLWGSVGFIFTVVIIGSLFDWLSLDIYPVVVVVMMTGIVGASLWVPNAQPVIHGNHFTGDSFLKQLRSHGVLTFYACVALMQMSHGPYYTFLTLYLKHFGYSRGVIGMLWALGVVAEVLMFLAMSRILARLSVQLVLLASFLLAAIRWLLLGSFAEIFWVLLLAQIMHAVTFASFHAAAIAFVQLSFGSRQQGQGQALYAALAGTGSALGALYSGYSWNLLGPTLTFNIASVAALAATVIISLRLQETH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMQ0</accession>
    <name>A0A481QMQ0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Cell division topological specificity factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">minE</name>
        <name evidence="5" type="ORF">C3B55_00826</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites. This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the MinE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00826"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032955">
        <property type="term" value="P:regulation of division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00262">
        <property type="entry name" value="MinE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005527">
        <property type="entry name" value="MinE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036707">
        <property type="entry name" value="MinE_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03776">
        <property type="entry name" value="MinE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55229">
        <property type="entry name" value="SSF55229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01215">
        <property type="entry name" value="minE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="84" mass="9704" checksum="2C924B5DEDD7F553" modified="2019-06-05" version="1">MKLFNSFRANKKFNTALVAKERLQIIVAHERGQRSTLNYLPILQKELVEVIRKYANINNDDVHIALEHDGSCSILELNIILSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMQ1</accession>
    <name>A0A481QMQ1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH-type transcriptional regulator MetR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cynR</name>
        <name evidence="8" type="ORF">C3B55_00551</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00551"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08441">
        <property type="entry name" value="PBP2_MetR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037406">
        <property type="entry name" value="MetR_PBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="7">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="305" mass="34320" checksum="6321DB7D7F2A432A" modified="2019-06-05" version="1">MLEIRHLKTLHALRETNSLVEAAERLHLTQSALSHQFRELEERLGVLLFVRKTKPLRFTSAGLRLLLLADATLPLLRGAERDITRLVGGTAGRLHMAIECHSCFQWIMPAINQFRDAWLEVELDLASGFAFAPLPALARGDLDLVVTSDPLELVGITYVPLFTYEVMLAVANQHQLASKPYITPEDLLIETLITYPVERDRLDIFTRFLEPANVEPAQVRTSELTVMIMQLVASGRGVCGIPHWALHEYSSRGYVQAKRLSEKGLFATLYAGIRADMLDAPYMRDFLLTAKDMSFSTLDIVNAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMR1</accession>
    <name>A0A481QMR1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="4 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nrdA</name>
        <name evidence="14" type="ORF">C3B55_00887</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="4 12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00887"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="PTHR11573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="SSF48168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="32"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="149"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX82196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="964" mass="108441" checksum="7DE78B348EBBA788" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMS8</accession>
    <name>A0A481QMS8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UDP-N-acetyl-alpha-D-glucosamine C6 dehydratase</fullName>
            <ecNumber evidence="4">4.2.1.135</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pglF</name>
        <name evidence="4" type="ORF">C3B55_00685</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.135" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00685"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05237">
        <property type="entry name" value="UDP_invert_4-6DH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003869">
        <property type="entry name" value="Polysac_CapD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02719">
        <property type="entry name" value="Polysacc_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_2" evidence="3">
        <location>
            <begin position="301"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="665" mass="73983" checksum="5D2C29EFC789DDB0" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMT2</accession>
    <name>A0A481QMT2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00868</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00868"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="62" mass="6566" checksum="93E3CDBEE47FD068" modified="2019-06-05" version="1">MRDAGVGGPPAEVVITHGCVDALQMSLRVLTHSGDLIAAEVANLLWCIAISRCAWSQGYLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-08-12" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMU1</accession>
    <name>A0A481QMU1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleoside-specific channel-forming protein, Tsx</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nucleoside-specific channel-forming outer membrane porin (Tsx) (TC 1.B.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00822"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018013">
        <property type="entry name" value="Channel_Tsx-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036777">
        <property type="entry name" value="Channel_Tsx-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03502">
        <property type="entry name" value="Channel_Tsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111364">
        <property type="entry name" value="SSF111364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019732933" evidence="2">
        <location>
            <begin position="23"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="262" mass="30435" checksum="A2DC05397B15D1A1" modified="2019-06-05" version="1">MKFMFKSLILAGSILASWQTIAGDLLQWQNNSLTYLYGNSFTINPSIQQTLTLFEHVDAWQYGDNFLFIDHVLYNGKGDNSLGSNTYYGEFTPRLSFSKIFNKKISFGLIQDVLLAMTYEFGKDNHTTYLVGPGFDLNVPGFDYFQLNFYQRHPTGDRPGDHVWQITPVWAYTMPIGNSDVLIDGYIDWEVNNDKNQRGTHHANLHFNPQIKYDLGRALHWDAKHLYVGFEYDFWKNKYGIDDNGTIKTNQNTTNFLVKYHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMW4</accession>
    <name>A0A481QMW4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Teichoic acids export ATP-binding protein TagH</fullName>
            <ecNumber evidence="6">3.6.3.40</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tagH</name>
        <name evidence="6" type="ORF">C3B55_00843</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.40" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00843"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03220">
        <property type="entry name" value="ABC_KpsT_Wzt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10147">
        <property type="entry name" value="Wzt_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015860">
        <property type="entry name" value="ABC_transpr_TagH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029439">
        <property type="entry name" value="Wzt_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14524">
        <property type="entry name" value="Wzt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="32"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="69"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="452" mass="51228" checksum="8D76172E94012FF2" modified="2019-06-05" version="1">MSSNNVITVQNLSKCYQLYAKPYDRLKQSIYPRIQEFFGIQKKQYFNEFWSLRDVSFEIKKGETVGIIGRNGSGKSTLLQVICGTLSQTAGSSKTNGRIAALLELGSGFNQELTGRENIYMNGAIMGLNRQKIEDRFDDIAAFADIGLFLEQPVKTYSNGMFVRLAFACNIMSDPEIMIIDEALSVGDMNFHAKCMTALMQIKESGATILFVSHDMGTVKSLCSRCIYLDNGKIVAVGSSADVAELYIRSMHEAMNAEHRKLSCALKPFAKSINLIEATNRLPIIAEKVFRVDHEFEARVAQHRYGTGQVKARSVILLDMNNEFITFLDFNQQVKIRVHFEAYTEKFVTLNVSVFDEKKNNITGCGFQHVNQPYLLTRPGGRYLAEYIFRMPLQEGCYSLRINISSVIVDNESVEFLDLINDAVIFHVARWENARVWSQVHQFAKLKLEELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMW8</accession>
    <name>A0A481QMW8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(R)-specific enoyl-CoA hydratase</fullName>
            <ecNumber evidence="2">4.2.1.119</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">phaJ</name>
        <name evidence="2" type="ORF">C3B55_00902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.119" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00902"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="156" mass="17299" checksum="9C5D29A11304E6C8" modified="2019-06-05" version="1">MIQITNIPYQALKISQTASYSKTVNELDIQLFSVISGDYNKVHLDAEYAKATLFKERIAHGMFSGAMISAAIACKLPGPGTIYISQQMRFKKPVKIGDTLTVRLKILEKLPKFRVRIATCVFNQSDELVVDGEAEILAPRKQQVVTLNELPSISIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMX3</accession>
    <name>A0A481QMX3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytoskeleton protein RodZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rodZ</name>
        <name evidence="3" type="ORF">C3B55_00955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00955"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025194">
        <property type="entry name" value="DUF4115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13464">
        <property type="entry name" value="DUF4115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="322" mass="34599" checksum="EF2E0AAC198F46AA" modified="2019-06-05" version="1">MKAVHQEVIAANRINPGETLRRTRESNGWSLAEVALKLNLTAISLSYLEAGAFDKLPGHTFARGYIRAYAKLLGINQTVLVQEFDQFTGTDSHGSNVHGLSCIEEPARVLHTILRIFSLLLLIVVIGGGFVWWQEQASQRGKDITNNAIEHVEVESVDGATRIHSLYEPKDQAVIQKQVKLESVAAPAIIAVVSAPALSATATIPIAQMHMRTALESALSATVLVATAAPAVLLPATPVLISDDGYIQITFIADCWTKVTDDNGQVLFSGLKHKGDTLYQGGKPPLTLRLGFARGAQVAYNGHSVNVMPFTSGETASLKLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMY9</accession>
    <name>A0A481QMY9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Adenine phosphoribosyltransferase</fullName>
            <shortName evidence="10">APRT</shortName>
            <ecNumber evidence="5 10">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">apt</name>
        <name evidence="12" type="ORF">C3B55_00855</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="5 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00855"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="11">
        <location>
            <begin position="34"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="182" mass="20174" checksum="4A8EAC7679A6842D" modified="2019-06-05" version="1">MTFDAFDIKSLIRTVIDFPKPGVIFRDITPLFQSPRAFRLTADSFAQRYVEADFTHIGAMDARGFLIGSIIAYQLNKPLILFRKQGKLPARVLAESYQTEYGEAFLEVHADSLGNGDSVLMFDDLIATGGTLIAAANLVHRIGAKIFEVAAIIDLPELGGSQRLEDMKIPTFFLTQFTLTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMZ3</accession>
    <name>A0A481QMZ3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pitA</name>
        <name evidence="7" type="ORF">C3B55_00739</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00739"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="491" mass="53244" checksum="452278FE915D6CD1" modified="2019-06-05" version="1">MIDLFSGLDAWILVGLLLALAFVLAFEFINGFHDTANAVATVIYTKAMPPHLAVFFSGVFNFLGVLLGGVGVAYAIVHLLPVDLLINVNTSHGLAMVFSLLAAAITWNLGTWYFGIPASSSHTLIGSILGVGLTNAMINDIPLADSINWQKAIDIGASLVLSPLAGFLVATIVLVGLQWWRPLSKMHKTPDQRRKFDDKKHPQFWNRLVLVISAIAVSFVHGSNDGQKGIGLIMLVLIGIVPTQFVLNLGSTTYQIERTRDATIHLSQFYQRNNATLEDFLAPSSSIRDDLPGKLQCNQQPTKMTIKALIETLQGVSDYHTLNSDNRIEVRRYLLCLDDTAKKVSKLPILDTSEKSDLDKLRKDLTATTEYAPFWIILTVALAIGLGTMVGWRRVVLTIGEKIGRQGLTYAQGMSAQITTASMIGLANIFSLPVSTTHVLSSGVAGTMVANKSGLQGNTVRTILMAWVLTLPMTAGLSAGLFWLASKALSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMZ4</accession>
    <name>A0A481QMZ4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dihydroorotate dehydrogenase (quinone)</fullName>
            <ecNumber evidence="5">1.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DHOdehase</fullName>
            <shortName evidence="5">DHOD</shortName>
            <shortName evidence="5">DHODase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Dihydroorotate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">pyrD</name>
        <name evidence="7" type="ORF">C3B55_00860</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-dihydroorotate + a quinone = a quinol + orotate</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="5">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00860"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00225">
        <property type="entry name" value="DHO_dh_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 5" id="KW-0288">FMN</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHO_dh" evidence="6">
        <location>
            <begin position="46"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="110"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="245"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="61"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="317"/>
            <end position="318"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="341" mass="37005" checksum="2ED124F818C5C3FC" modified="2019-06-05" version="1">MYTLIRKLLFKLSTESSHDLSLELIGTGGRLGLNRLICKKPAKILVSVMGLKFPNPVGLAAGLDKNGIAIDGFAQMGFGFVEIGTVTPRPQFGNPKSRMFRLQKAEAIINRMGFNNLGVDYLLSRIRTAKYNGILGINIGKNFDTPIERAIDDYLICLNKVYSWASYITVNISSPNTPSLSSLQFGDSLKRLLEALSRRQEYLAVYYGKRVPLVIKISPDIHDKEIVLIVQALINFGMDAVISTNTTISRVGVEGLRHGNEAGGLSGAPLRDKSTHIVKVLAAELAGRLPIIAVGGITEGKHAAEKIAAGASLVQLYSGFVYKGPALIRESVEAIIALLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN25</accession>
    <name>A0A481QN25_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Elongation factor P</fullName>
            <shortName evidence="7">EF-P</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">efp</name>
        <name evidence="10" type="ORF">C3B55_00680</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the elongation factor P family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00680"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00141">
        <property type="entry name" value="EF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015365">
        <property type="entry name" value="Elong-fact-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020599">
        <property type="entry name" value="Transl_elong_fac_P/YeiP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001059">
        <property type="entry name" value="Transl_elong_P/YeiP_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011768">
        <property type="entry name" value="Transl_elongation_fac_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30053">
        <property type="entry name" value="PTHR30053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01132">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09285">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005901">
        <property type="entry name" value="EF-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01185">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00841">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EFP" evidence="9">
        <location>
            <begin position="67"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Elong-fact-P_C" evidence="8">
        <location>
            <begin position="131"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX82004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="190" mass="21321" checksum="81CE9753FEE062E6" modified="2019-06-05" version="1">MKTGKELKPGIVIRLETDPWLVRKAEFTKSGRNSAIMKTKLTNLLTGYKTEVVYSADDKLDDVVLDRKEVTLSFISGGMYTFIDIMDYAMYELSAEALEAILPFIEEGMQDVCNAIFFEGRLVSIELPTTIVRKIAYTERSARGDTSGKVMKPARLANGTTLQVADFIEIDDLIEIDTREGGSYKSRIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="5" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN40</accession>
    <name>A0A481QN40_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00900"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="214" mass="24382" checksum="3677FE6A0FEA60CB" modified="2019-06-05" version="1">MLNTNHMNTKVLSVFDFDGTLTYHDSFLQFLKFAFGHEKFYIGIVKLAVPGIRFLVRQIGHNELKEQLISIFMTGLEKAWIQRQAEVYCQRYWTKLMRPDGLQSVADEVKSGAIVTLCSASPAVILQPFTDRLGIQLIGTELEVVNEVLTGKLMGNNCRCENKVLRLKSIYGDLGKYRLRAWGDTRGDLELLAAAQDAHFQSFHSKKKKNRALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-02-10" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN67</accession>
    <name>A0A481QN67_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gap</name>
        <name evidence="4" type="ORF">C3B55_00712</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00712"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="3">
        <location>
            <begin position="132"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="482" mass="53367" checksum="33E08073E1C707D7" modified="2019-06-05" version="1">MTQKPDQYLSEWIDREALAEAMIPLIGQLYRSNNVSSSIYGYSLVNQSVIAILKAHRFAHYRSFDNSELSIHETFPLLKAISELELSSVTVDLGKLAYKFRKKSASYSAKQFAYEEMADVVGQQYTIASKGADVVLYGFGRIGRLIARIMIEKAGSGGELRLRAIVVRKGAENDLVKRASLLRRDSVHGHFNGIIVVDDDNNTINANGNLIQIIYAQNPAEVDYTQYGIKNALLVDSTGAWRDAKGLSQHLACQGINRVVLTAPGKGKLKNIVYGINHNEIIADDKIVSAASCTTNAIVPVLKVVNDKFGIVNGHIETVHAYTNDQNLIDNFHKGNRRGRSAVLNMIITETSAAIAVTKVLPELAGRITGNAIRVPTPNVSMAILNLNLAKAATREEMNEYLRYIVLHTDLHKQIDLVHSHEVVSTDFIGSRHTGVVDTEATIIQDNRVVLYVWYDNEFGYSCQVVRVMENMAGINLPTFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN73</accession>
    <name>A0A481QN73_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00999"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="41" mass="4652" checksum="E9FE3C3600CAAFCF" modified="2019-06-05" version="1">MLALFNFANQINQSMINVLSIALLRINSLNFQLLLVVKADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN81</accession>
    <name>A0A481QN81_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00932</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00932"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="42" mass="4898" checksum="0403EFE7F747502D" modified="2019-06-05" version="1">MRRIDSHAEDFARPCDLVFFGKTLIDVAYASYYAYLYVSDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN85</accession>
    <name>A0A481QN85_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="4 8">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">fmt</name>
        <name evidence="11" type="ORF">C3B55_01009</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-tRNA(fMet)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="4 8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01009"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08704">
        <property type="entry name" value="Met_tRNA_FMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00182">
        <property type="entry name" value="Formyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037022">
        <property type="entry name" value="Formyl_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044135">
        <property type="entry name" value="Met-tRNA-FMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="9">
        <location>
            <begin position="6"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl_trans_C" evidence="10">
        <location>
            <begin position="207"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Tetrahydrofolate (THF) binding" evidence="8">
        <location>
            <begin position="113"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="316" mass="34042" checksum="1360651FD459480B" modified="2019-06-05" version="1">MTEPLRIVFAGTPTFAAEHLKALLASPYHVIAVYTQPDRHAGRGQKLMSSPVKQLALKHNIPVLQPVTLHNADAQLELAALMPDLLVVVAYGLILPQAVLDIPRLGGINSHASLLPRWRGAAPIQRAVEAGDSESGVTVMRMEEGLDTGPILLKVTTPIINKDTGGSLHDRLAKLGPPAMIQTITSLASNTVKHEVQDNSLVTYAHKLHKNEACINWNRPAVELERLIRAFNPWPLYHSILNGKILKVLDANLVNGKGAPGEIIGTSKDGLLVACGEQALCLTRLQLPGGKALSFNCIFNSRREKISLGTVLGVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN98</accession>
    <name>A0A481QN98_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Fifty-four homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">ffh</name>
        <name evidence="9" type="ORF">C3B55_00943</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="7">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00943"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004780">
        <property type="entry name" value="SRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF7">
        <property type="entry name" value="PTHR11564:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00959">
        <property type="entry name" value="ffh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54" evidence="8">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="190"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="248"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="453" mass="49426" checksum="ABB07C92EBBAA6B0" modified="2019-06-05" version="1">MFKNLTDRFSQTLRHVSGKAKLTENNIKDTLREVRIALLEADVALPVVKDFINSIQNRAVGTEVSRSLTPGQVFVKIVQSELESMMGVVNEDLNLSTVPPAVLLMAGLQGAGKTTTAGKLALLLKKNKKKSVMLVSVDVYRPAAIKQLEMLAIQVGATFFSSTMSQKPVDIAGDAIKEAKIKCIDVVIVDTAGRLHIDEEMMMEIKALHAVINPIETLFVVDAMTGQDSVNIAKAFGEALPLTGVILTKVDGDARGGAALSVKAITGKPIKYIGMGEKSEALELFHPKRIASRILGMGDVLSLIEQAEATFEKDKADKLIKKLKKGNGFDLEDFRDQLHQMRNIGGLSSLIDKIPKIDGVNLLQIGNVHGAAEKQLKQMEVIINSMTTYERRNPELISASRKRRIAMGSGTQVQDIGRLIKQHKQMQNMMKTFSTKGGMAKIMHSISGSVSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNB1</accession>
    <name>A0A481QNB1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-binding protein IscA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Iron-sulfur cluster assembly protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">iscA</name>
        <name evidence="6" type="ORF">C3B55_00962</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00962"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="FeS_cluster_insertion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011302">
        <property type="entry name" value="IscA_proteobacteria"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02011">
        <property type="entry name" value="IscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00049">
        <property type="entry name" value="TIGR00049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S_biosyn" evidence="5">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="107" mass="11837" checksum="63A5CA423B3B3585" modified="2019-06-05" version="1">MAISMTKAAARHVRRSLDRRGKGAGVRLVVRTAGCSGLAYMLEFVDEVVAEDQVFESHGEKVIIDHKSLTYLDNTELDFIKEGLNEGFKFNNPNVRGECGCGESFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNB2</accession>
    <name>A0A481QNB2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)</fullName>
            <ecNumber evidence="6">1.17.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">ispG</name>
        <name evidence="7" type="ORF">C3B55_00954</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:43604"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:10623"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the IspG family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.3" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00954"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00096"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046429">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00159">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016425">
        <property type="entry name" value="IspG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004588">
        <property type="entry name" value="IspG_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30454">
        <property type="entry name" value="PTHR30454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04551">
        <property type="entry name" value="GcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004640">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00612">
        <property type="entry name" value="ispG_gcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="369" mass="39927" checksum="8E7785972095C951" modified="2019-06-05" version="1">MRGEYLIKRRVSRKIWVGSVPIGGDAPIAIQSMTNSDTNDIAATVVQINRLEAAGVDIVRVSVPDMRAAEIFGCIKKLVKVPLVADIHFDYKIALRVAELGVDCLRINPGNIDRKDRVRAVVNAARDRGIPIRIGVNAGSLEKDLQKKYGKPTPAALVESALRHVEHLERLNFHDFKVSVKASDVLIAVEAYRLLAKEIMQPLHLGITEAGGLRSGTVKSAVGIGMLLAEGIGDTIRISLAADPVEEVNVGYDILKSLCLRSRGINFIACPSCSRQNFNVVKTISELELRLEDLLVPLDVAVIGCVVNGPGEAKEAHVGLTGGKPNLIYIDGKPSHKLTNNSLVNDLEKLIRKKAAEKAIADANLITLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNS6</accession>
    <name>A0A481QNS6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secD</name>
        <name evidence="11" type="ORF">C3B55_00969</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecF. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00969"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_B">
        <property type="entry name" value="SecD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005791">
        <property type="entry name" value="SecD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027398">
        <property type="entry name" value="SecD-TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13721">
        <property type="entry name" value="SecD-TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
        <property type="entry name" value="2A0604s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01129">
        <property type="entry name" value="secD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="587"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="SecD-TM1" evidence="10">
        <location>
            <begin position="2"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="620" mass="68197" checksum="015BCD11FA3D268A" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QP55</accession>
    <name>A0A481QP55_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00931"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="55" mass="6792" checksum="440505B6103C1D75" modified="2019-06-05" version="1">MRWFKEYRGAISVLTECMQRFHLNSMVLYMLNEQCSFDYPGELYLESHHNLQKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QPD3</accession>
    <name>A0A481QPD3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome bo(3) ubiquinol oxidase subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome o ubiquinol oxidase subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Oxidase bo(3) subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquinol oxidase polypeptide IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquinol oxidase subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">cyoD</name>
        <name evidence="16" type="ORF">C3B55_00975</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooctamer of two A chains, two B chains, two C chains and two D chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase bacterial subunit 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00975"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005171">
        <property type="entry name" value="Cyt_c_oxidase_su4_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014210">
        <property type="entry name" value="Cyt_o_ubiqinol_oxidase_su4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03626">
        <property type="entry name" value="COX4_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02847">
        <property type="entry name" value="CyoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX82274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="110" mass="12303" checksum="CA3F4B8278E7EC33" modified="2019-06-05" version="1">MVNSCSYNYNASHGSIVSYTIGCVLSVILTLVPFCLVIYPTLSKLSTLIIILVCAVIQVMVHLLYFLHLDNTKGQRDNLIALIFTSLVILLMVGLSIWIMFSMHTFMMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QJX7</accession>
    <name>A0A481QJX7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase subunit c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP synthase F(0) sector subunit c</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">F-type ATPase subunit c</fullName>
            <shortName evidence="13">F-ATPase subunit c</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">atpE</name>
        <name evidence="15" type="ORF">C3B55_00006</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Key component of the F(0) channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00006"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005953">
        <property type="entry name" value="ATP_synth_csu_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01260">
        <property type="entry name" value="ATP_synt_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 13" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="14">
        <location>
            <begin position="10"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="site" description="Reversibly protonated during proton transport" evidence="13">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX81381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="86" mass="8781" checksum="437DDB0FBEF5905B" modified="2019-06-05" version="1">METVVGLTAIAVALLIGLGALGTAIGFGLLGGKFLEGAARQPEMVPMLQVKMFIVAGLLDAVTMIGVGIALFFTFANPFVAQIASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK46</accession>
    <name>A0A481QK46_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00068"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="72" mass="8240" checksum="CD69E29E39DE5598" modified="2019-06-05" version="1">MAAECLNRLLIGKFNMMFMKVKLVWQGARKEPFNLDYAVYHSELLLLADIGEERYAAFFGVLIIHYLSVVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-02-10" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKC3</accession>
    <name>A0A481QKC3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">YGGT family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YggT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00085"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003425">
        <property type="entry name" value="CCB3/YggT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33219">
        <property type="entry name" value="PTHR33219"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02325">
        <property type="entry name" value="YGGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="195" mass="22068" checksum="95A11242884EEA09" modified="2019-06-05" version="1">MLGINDAAIFITQTIGSLYLLIVLMRFILQLVRANFYNPLCQFIVKATQPLLKPLRNVTPNLFGLDMSSLLLALLLQIVLFVVIMMLHGYQAFTVLLLPWGLIGIFSLFLKIIFWSMIISVILSWVTPDSRSTGAELVTQITEPVLAPFRRLIPNLSGIDISPIFVFIVMQLLQSWVIPRLAYYALMPKELFGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-04-07" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKC5</accession>
    <name>A0A481QKC5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative pre-16S rRNA nuclease</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">yqgF</name>
        <name evidence="4" type="ORF">C3B55_00090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YqgF HJR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00090"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000967">
        <property type="term" value="P:rRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16964">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00651">
        <property type="entry name" value="Nuclease_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317">
        <property type="entry name" value="PTHR33317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00250">
        <property type="entry name" value="RNAse_H_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="YqgFc" evidence="3">
        <location>
            <begin position="4"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="141" mass="15936" checksum="053D29403D310934" modified="2019-06-05" version="1">MSLRLILGFDYGTKKIGVAIGQLITGQARELCTLKAKNGTPEWNKVEALIKEWKPDAIIVGLPLNMDGTHGDMCLRAAKFARRLSRRYSLPFYTYDERLTTVEAKFERHNRSGQIGNYRNDPVDAIAATLLLQGWLNDKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKE0</accession>
    <name>A0A481QKE0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease PH</fullName>
            <shortName evidence="4">RNase PH</shortName>
            <ecNumber evidence="4">2.7.7.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">rph</name>
        <name evidence="7" type="ORF">C3B55_00029</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>phosphate + tRNA(n+1) = a ribonucleoside 5'-diphosphate + tRNA(n)</text>
            <dbReference type="Rhea" id="RHEA:10628"/>
            <dbReference type="Rhea" id="RHEA-COMP:17343"/>
            <dbReference type="Rhea" id="RHEA-COMP:17344"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:173114"/>
            <dbReference type="EC" id="2.7.7.56"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homohexameric ring arranged as a trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.56" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00029"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009022">
        <property type="term" value="F:tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11362">
        <property type="entry name" value="RNase_PH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00564">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002381">
        <property type="entry name" value="RNase_PH_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018336">
        <property type="entry name" value="RNase_PH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01966">
        <property type="entry name" value="RNasePH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01277">
        <property type="entry name" value="RIBONUCLEASE_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="RNase_PH" evidence="5">
        <location>
            <begin position="11"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="RNase_PH_C" evidence="6">
        <location>
            <begin position="159"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate (substrate) binding" evidence="4">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <note>substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="240" mass="26204" checksum="06762A2251D5A302" modified="2019-06-05" version="1">MKRLNGRHANQLRSIRITRNYTKYAEGSALIEFGDTKVICTVSVENGVPRFLKGQGQGWLTAEYGMLPRATGTRNQREAIRGKQGGRTLEIQRLIGRSLRAALDMSKLNDVTLYVDCDVIQADGGTRTASITGAMVALVDALKVMKKCGDLKANDLLKHMIASVSVGMYKGEPMLDLDYLEDSSAETDLNVVMTSTGSFIEIQGTAEITPFQLADLNAMLTLAQKGITEIFKLQNAILAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKL0</accession>
    <name>A0A481QKL0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Glucose-inhibited division protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">mnmG</name>
        <name evidence="11" type="synonym">gidA</name>
        <name evidence="14" type="ORF">C3B55_00002</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9 11">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the MnmG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00002"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PTHR11806:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00136">
        <property type="entry name" value="gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="GIDA" evidence="12">
        <location>
            <begin position="8"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="GIDA_C" evidence="13">
        <location>
            <begin position="402"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="630" mass="69789" checksum="7A7921786AD38806" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKN8</accession>
    <name>A0A481QKN8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00188"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="41" mass="4430" checksum="600518B508EDE7E9" modified="2019-06-05" version="1">MALKVSVICAYYSGIASMLRPHKELSGAKEASIQPLTNLHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKP7</accession>
    <name>A0A481QKP7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipopolysaccharide heptosyltransferase 1</fullName>
            <ecNumber evidence="2">2.-.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rfaC</name>
        <name evidence="2" type="ORF">C3B55_00198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.-.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00198"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008920">
        <property type="term" value="F:lipopolysaccharide heptosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009244">
        <property type="term" value="P:lipopolysaccharide core region biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011908">
        <property type="entry name" value="LipoPS_heptosylTferase-I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02193">
        <property type="entry name" value="heptsyl_trn_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="353" mass="39344" checksum="CF49F97A20440774" modified="2019-06-05" version="1">MRVLIIKTSSLGDVIHVLPALTEAMLAIPGIRFDWVVEEGFAELPTWHPAVDKVIPVAIRRWRKHIWWTIKSGEWQSFKKSIQSTKYDLVIDAQGLLKSAWLTRYVSAPVAGFDKDSAREPIAACFYSRRLAVPPTQHAVERLRQLFAAALGYNLADGSGNYGLSVHKLYSLTPKKPFVLLLHGSTWDTKRWPEIYWRELAKHLADHGVDVKLPWGNAVEKVRAERLAIGLENAEVLPKLNLGGVARVLTGALACVAVDTGFGHLAAALNVPTISLFSSTNPILTGTYGKRQIHLSSDFPCAPCLQKHCTYQPTYDDLRLFDMKLELPLCFTRLSPAHVASRLSMLLFTDGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKQ0</accession>
    <name>A0A481QKQ0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-hydroxythreonine-4-phosphate dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.262</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">4-(phosphohydroxy)-L-threonine dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pdxA1</name>
        <name evidence="4" type="synonym">pdxA</name>
        <name evidence="5" type="ORF">C3B55_00118</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NAD(P)-dependent oxidation of 4-(phosphooxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphooxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>4-(phosphooxy)-L-threonine + NAD(+) = 3-amino-2-oxopropyl phosphate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32275"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="EC" id="1.1.1.262"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="4">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="4">Binds 1 divalent metal cation per subunit. Can use ions such as Zn(2+), Mg(2+) or Co(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">The active site is located at the dimer interface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PdxA family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.262" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00118"/>
    <dbReference type="UniPathway" id="UPA00244">
        <property type="reaction ID" value="UER00312"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050570">
        <property type="term" value="F:4-hydroxythreonine-4-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00536">
        <property type="entry name" value="PdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037510">
        <property type="entry name" value="PdxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005255">
        <property type="entry name" value="PdxA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30004">
        <property type="entry name" value="PTHR30004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04166">
        <property type="entry name" value="PdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00557">
        <property type="entry name" value="pdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="329" mass="35252" checksum="E9844B5D3A779A53" modified="2019-06-05" version="1">MKPKCFAFTPGEPAGIGPDLCLLLTSYPQPHNLIAITSLDLLIERAAQLGLAVKFLDVAPGKWPTMPAPVGSLYVWNTPLATKVVAGQLNKANVTFVLETITRAVQGCIHGDFAGMITAPVHKGIINESGIAFFGHTELIAKLTNTSQAVMMLATSGLRVALVTTHLPLRDIANAITATRLECAIRILYSDLQQKFGISQPRILVCGLNPHAGESGYLGHEEIDIIDPTLKQLRQEGIDLCGPLPADTLFTQKYLKHCDVVLAMYHDQGLPVLKYKGFGVAVNVTLGLPIIRTSVDHGTALGLAGSGKIDTGSLYLALETAYKMAEIHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKR6</accession>
    <name>A0A481QKR6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Outer membrane efflux protein BepC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bepC</name>
        <name evidence="5" type="ORF">C3B55_00135</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00135"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010130">
        <property type="entry name" value="T1SS_OMP_TolC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01844">
        <property type="entry name" value="type_I_sec_TolC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019857521" evidence="4">
        <location>
            <begin position="22"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="155"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="453" mass="50577" checksum="6414E1329FCD71A1" modified="2019-06-05" version="1">MYLHLFKAIPFVVLTVSFAQAQTLPLAMQQALDVRPEIQAGVNSRLAADYQLRAAQGGYLPHVDINASYGREGADNSTTRSNNSLNGNHWDTLNRQESSLRLQQMIFDGFATSNEVGRQQAEVNSRAYSLLSTTERTGLTVARVYLDVLTRREFVRLAEENLRNHERIYDQIKLRTQSGVGNGADQDQAEARLAQVRNNLLTEQTNLADSQVNYLSAVGQMPNQLERPASFISTLPANLNEARSQMLDNSPVLRSAESDISAAESQYEAAKSPLYPRFDAEFSTNADNNVAGDASHSSGWKAIIRMRFNLFAGGSNKADLQSKSYLVNQALDIRNNALRQLNEELCLAWNALNNANAQLPVAQQYVDHSTRVRTSYRQQFSLSQRTLLDLLDSENELFTASRRLEEIKNIQLFSQYRIKATMGELLKSQGVVAPIASVVQNYIKPKVQLPGMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKS7</accession>
    <name>A0A481QKS7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00158"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="99" mass="11170" checksum="9845642424603F0E" modified="2019-06-05" version="1">MSHGSYAIVGATFIPKCWIPIIQLLTYISESELIDYGYALFVLIIMWRTCRVAFCLLAGRLSGEAALIKISNVMIAKLHTCQYRITHSANYASYSWLCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKT3</accession>
    <name>A0A481QKT3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional protein PutA</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="2">Proline dehydrogenase</fullName>
                <ecNumber evidence="2">1.5.5.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">Proline oxidase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="2">Delta-1-pyrroline-5-carboxylate dehydrogenase</fullName>
                <shortName evidence="2">P5C dehydrogenase</shortName>
                <ecNumber evidence="2">1.2.1.88</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="9" type="primary">putA</name>
        <name evidence="9" type="ORF">C3B55_00192</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Oxidizes proline to glutamate for use as a carbon and nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + L-glutamate 5-semialdehyde + NAD(+) = 2 H(+) + L-glutamate + NADH</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a quinone + L-proline = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the proline dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2"/>
    <dbReference type="EC" id="1.2.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00192"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00373"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:1-pyrroline-5-carboxylate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006561">
        <property type="term" value="P:proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:proline catabolic process to glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025703">
        <property type="entry name" value="Bifunct_PutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005933">
        <property type="entry name" value="Delta1-pyrroline-5-COlate_DH-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041349">
        <property type="entry name" value="PRODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024090">
        <property type="entry name" value="PRODH_PutA_dom_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024089">
        <property type="entry name" value="PRODH_PutA_dom_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024082">
        <property type="entry name" value="PRODH_PutA_dom_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14850">
        <property type="entry name" value="Pro_dh-DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18327">
        <property type="entry name" value="PRODH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000197">
        <property type="entry name" value="Bifunct_PutA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81935">
        <property type="entry name" value="SSF81935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01238">
        <property type="entry name" value="D1pyr5carbox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PRODH" evidence="8">
        <location>
            <begin position="87"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Pro_dh-DNA_bdg" evidence="7">
        <location>
            <begin position="146"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Pro_dh" evidence="6">
        <location>
            <begin position="270"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="655"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="881"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000197-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="1317" mass="143441" checksum="57DE9AF9326787F5" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKU0</accession>
    <name>A0A481QKU0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Spermidine/putrescine import ATP-binding protein PotA</fullName>
            <ecNumber evidence="8">7.6.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">potA</name>
        <name evidence="10" type="ORF">C3B55_00060</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.11" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00060"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015417">
        <property type="term" value="F:ABC-type polyamine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005893">
        <property type="entry name" value="Sp_pt_ABC_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01187">
        <property type="entry name" value="potA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="23"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="55"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="380" mass="42587" checksum="D9F9DD9F4E702D73" modified="2019-06-05" version="1">MAIASGAYKKAIDGDQTPKKVLVKIDRVTKKFDETIAVDDVSLQIKKGEIFALLGGSGSGKSTLLRMLAGFECPTEGRIYLDCVDITDMPPYKRPINMMFQSYALFPHMTVAQNIAFGLQQDKISRIEIDARVAEMLKLVQMIQYAKRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRSQMQLELVEIIERVCVTCVMVTHDQEEAMTMAERIAIMHQGWIAQIGSPIDIYETPTSRLVCEFIGSVNIFDTQVVDDAEGHALLKCPDLDRDIYVGYGIATAVEDKSLIYAIRPEKLLVTIEMPTREHNWSSGKVHDIAYLGGHSVFYVELPSGKLVQSFVANAERRGQRPTWGDQVYVWWEADSGVVLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKU8</accession>
    <name>A0A481QKU8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Biotin carboxyl carrier protein of acetyl-CoA carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">accB</name>
        <name evidence="7" type="ORF">C3B55_00251</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00251"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001249">
        <property type="entry name" value="AcCoA_biotinCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01071">
        <property type="entry name" value="ACOABIOTINCC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00531">
        <property type="entry name" value="BCCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="70"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="146" mass="15824" checksum="0229B8ABEC79059D" modified="2019-06-05" version="1">MDIRKIKKLIELLEASGIDELEIKEGEASVRISRQSKASAQQFYAPQMQATTAVAPAATRVATATTMLNSVVVRSPMVGTFYRTPAPTSPAFVEVGTTVQVGDTICIVEAMKMMNYITAETSGIIESIFVENGHPVEYDQPLLTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKW9</accession>
    <name>A0A481QKW9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Guanylate kinase</fullName>
            <ecNumber evidence="2 9">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">gmk</name>
        <name evidence="11" type="ORF">C3B55_00028</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for recycling GMP and indirectly, cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + GMP = ADP + GDP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2 9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00028"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:guanylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="10">
        <location>
            <begin position="6"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="13"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="206" mass="23734" checksum="208857F2C2084AE8" modified="2019-06-05" version="1">MTYSTGTLYIISAPSGTGKSSLIKALTDADERIRISISHTTRTMRPGEINGVHYYFVEHTEFIKMIKYGDFLECAEVFGKLYGTSQNHLQQTLDKGYDLILEIDWQGAKQVRQLIPKTHSIFILPPSIDTLYQRLTNRGEDSEESIAERMREAVHNMSHYVDYDYLIINDDFTHAINDLRAIVRTNQLQKQRQQHCFSTLLTELLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKX8</accession>
    <name>A0A481QKX8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">50S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">rpmE</name>
        <name evidence="9" type="ORF">C3B55_00183</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Binds the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00183"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00501">
        <property type="entry name" value="Ribosomal_L31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002150">
        <property type="entry name" value="Ribosomal_L31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027491">
        <property type="entry name" value="Ribosomal_L31_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042105">
        <property type="entry name" value="Ribosomal_L31_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33280">
        <property type="entry name" value="PTHR33280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01197">
        <property type="entry name" value="Ribosomal_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01249">
        <property type="entry name" value="RIBOSOMALL31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00105">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01143">
        <property type="entry name" value="RIBOSOMAL_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="85" mass="9694" checksum="2FEE38E9B0B4B169" modified="2019-06-05" version="1">MWLSKKFIMKTNIHPAYEIIEVTCSCGNKFETRSNLCKPLSTDVCNECHPFYTGKQKILNTGGRIQRFADRFGAFGKTPYVVLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKY5</accession>
    <name>A0A481QKY5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-demethoxyubiquinol 3-hydroxylase</fullName>
            <shortName evidence="7">DMQ hydroxylase</shortName>
            <ecNumber evidence="7">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">coq7</name>
        <name evidence="8" type="ORF">C3B55_00127</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydroxylation of 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol + AH2 + O2 = A + a 3-demethylubiquinol + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="7">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the COQ7 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00127"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="PTHR11237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 7 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="215" mass="24452" checksum="3318564C706395EA" modified="2019-06-05" version="1">MTTQRHYSPFDRLLMQVSIAMRTLLPFSSQPYRPSPAIMQPDTQMNETDTHHVACLMRINHTGEVCAQALYQGQALTAKRLQVRTAIEHTAEEEIDHLVWCEQRIQQLGSHPSILNPLFYGLSFSIGVATGLINDNISLGFMAATEHQVCQHLDKHLKQLPTEDKKSRAILEQMRRDEEHHAESALNTNGLHFPALVKFSMSILAKIMTKSTYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKZ4</accession>
    <name>A0A481QKZ4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">rplO</name>
        <name evidence="8" type="ORF">C3B55_00296</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00296"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_L15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="PTHR12934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L18e/L15P" evidence="7">
        <location>
            <begin position="28"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="145" mass="15238" checksum="F4F53835316322A1" modified="2019-06-05" version="1">MKLNDLSPAPGSRCKKQRQGRGIGSGLGKTGGRGHKGQKSRSGGAIAPGFEGGQQPLHRRLPKFGFVSMKAMKHAEVRLSELAKVEDNIITLQTLKDANVININVQSVKIMLSGKVTHAVTIGKGIGTTKGARLVIEAAGGKFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL21</accession>
    <name>A0A481QL21_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane-bound lytic murein transglycosylase B</fullName>
            <ecNumber evidence="3">4.2.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mltB</name>
        <name evidence="3" type="ORF">C3B55_00327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00327"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011757">
        <property type="entry name" value="Lytic_transglycosylase_MltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043426">
        <property type="entry name" value="MltB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031304">
        <property type="entry name" value="SLT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30163">
        <property type="entry name" value="PTHR30163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30163:SF0">
        <property type="entry name" value="PTHR30163:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13406">
        <property type="entry name" value="SLT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02282">
        <property type="entry name" value="MltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLT_2" evidence="2">
        <location>
            <begin position="40"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR611757-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="340" mass="38477" checksum="D028A4092439229E" modified="2019-06-05" version="1">MNISIQIMCNWVARYVFWIGLIGLLGTTQEMQAGDYDSSPQVVEFIREMTRDYGFTGEQLMGVFRETQRQQAILDAISRPAERVKQWKEYRTMFLTDVRVAMGVEFWSQHEDVLNRAEQKYGVPAQIIVAIIGIETFYGLNTGSYRVIDALSTLSFDYPPRAEFFRNELREFLLLAREEQVDSLTLKGSYAGAMGLPQFIPSSVRAYAVDFDGDGHINIWSNHGDAIGSVASYFKRHGWVAGEPVVISADVTGRHVYEGLTKGIEPVKTVGELRTLGWSSQNALRDAVLVTAFCLEGINGPEYWMGLKNFYAITRYNRSVMYAMAVYQLSDMLVKTRGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-08-12" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL24</accession>
    <name>A0A481QL24_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acyl-CoA esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">C3B55_00099</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00099"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="PTHR11049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="136" mass="15395" checksum="8A8BB7218258474A" modified="2019-06-05" version="1">MTMNFHTRKWVKPEDLNPNGTLFGGSLLRWIDEEAAIYAIVQLGNQRVVTKYISEINFASTSRQGDIIELGITATEFGHTSITLTCQVRNKITRKSILTVKKIVFVNLDKDNQPVPHGCTEIKYVKDQFPEDGVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL34</accession>
    <name>A0A481QL34_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor RpoD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Sigma-70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">rpoD</name>
        <name evidence="9" type="ORF">C3B55_00110</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts transiently with the RNA polymerase catalytic core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sigma-70 factor family. RpoD/SigA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00110"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00963">
        <property type="entry name" value="Sigma70_RpoD_SigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007631">
        <property type="entry name" value="RNA_pol_sigma_70_non-ess"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007127">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042189">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012760">
        <property type="entry name" value="RNA_pol_sigma_RpoD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028630">
        <property type="entry name" value="Sigma70_RpoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04546">
        <property type="entry name" value="Sigma70_ner"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03979">
        <property type="entry name" value="Sigma70_r1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02393">
        <property type="entry name" value="RpoD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="7">
        <location>
            <begin position="398"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="8">
        <location>
            <begin position="567"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="6">
        <location>
            <begin position="374"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-3" evidence="6">
        <location>
            <begin position="453"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-4" evidence="6">
        <location>
            <begin position="542"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="6">
        <location>
            <begin position="398"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="608" mass="69188" checksum="A919050FE7B66858" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL36</accession>
    <name>A0A481QL36_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00347"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="47" mass="5364" checksum="FC0B855267452969" modified="2019-06-05" version="1">MMPLDKKRKLKLFISLICKGCQNNILVFIKTHNGINPLVEKLQVIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL62</accession>
    <name>A0A481QL62_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">rplE</name>
        <name evidence="10" type="ORF">C3B55_00289</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00289"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_B">
        <property type="entry name" value="Ribosomal_L5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_L5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L5" evidence="8">
        <location>
            <begin position="25"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L5_C" evidence="9">
        <location>
            <begin position="84"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="179" mass="20378" checksum="504A0A79B193F691" modified="2019-06-05" version="1">MARLQEIYRKTISPKLKEELKLRSMMAVPRVTKVTLNMGLSEAIGDKKVIEHAVSDLEKIASQKAVVTYARKSIAGFKVREGWPIGVKVTLRRERMYEFLDRLLSIALPRVRDFRGLSVKSFDGHGNYSMGVKEQIIFPEIDYDKTDVIRGLDITLTTTAKSDNDGLALLRGFKFPFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL69</accession>
    <name>A0A481QL69_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rpsS</name>
        <name evidence="7" type="ORF">C3B55_00281</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00281"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_S19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="91" mass="10277" checksum="2936B1704CEE1FD3" modified="2019-06-05" version="1">MPRSLKKGPFIDLHLLKKIKVASAKNDRKPVKTWSRRSMILPQMVGLTIAVHNGRQHIPVLVNEDMVGHKLGEFAGTRTCRGHMADKKAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL72</accession>
    <name>A0A481QL72_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00299"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="48" mass="5461" checksum="AB6EE912A5739C4A" modified="2019-06-05" version="1">MAWNRATNNVKAELDLFDGRVDGYLPSKLFVITAIIILRPISWLIGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL81</accession>
    <name>A0A481QL81_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Outer-membrane lipoprotein LolB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">lolB</name>
        <name evidence="14" type="ORF">C3B55_00406</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the LolA protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the LolB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00406"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044874">
        <property type="term" value="P:lipoprotein localization to outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16326">
        <property type="entry name" value="LolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00233">
        <property type="entry name" value="LolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004565">
        <property type="entry name" value="OM_lipoprot_LolB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03550">
        <property type="entry name" value="LolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="SSF89392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00548">
        <property type="entry name" value="lolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019842770" description="Outer-membrane lipoprotein LolB" evidence="13">
        <location>
            <begin position="26"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="204" mass="23081" checksum="E0F678B8D5C3FEF7" modified="2019-06-05" version="1">MLLRQIIAFSLITLLVGCASHNALKYGTGADNQTQWKQHKDQLSNINYWQIDGKIGVHTPKASGSGTLSWLQRQDYYDIRLSGPLSLGTSHLTGRNGHESLAITNNGYLATVSLETLLEQQTGWKLPVSNLVWWIRGLPAPDSNSRIHLNGDSRPAMIEQDGWQIEYRSYVKQNGYLLPERIQLHSTDLNVKLVIKEWQPHKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-08-12" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL91</accession>
    <name>A0A481QL91_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable Fe(2+)-trafficking protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be a mediator in iron transactions between iron acquisition and iron-requiring processes, such as synthesis and/or repair of Fe-S clusters in biosynthetic enzymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fe(2+)-trafficking protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00157"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3880.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00686">
        <property type="entry name" value="Fe_traffic_YggX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007457">
        <property type="entry name" value="Fe_traffick_prot_YggX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036766">
        <property type="entry name" value="Fe_traffick_prot_YggX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36965">
        <property type="entry name" value="PTHR36965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04362">
        <property type="entry name" value="Iron_traffic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029827">
        <property type="entry name" value="Fe_traffic_YggX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111148">
        <property type="entry name" value="SSF111148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="92" mass="10760" checksum="D6A8EF33D9375F56" modified="2019-06-05" version="1">MTRTIMCRKYKEKLPGLDYPPYPGASGYDIYNHVSAKAWSDWLKHQTLIINAKRLNMTNAEDRKYLTREMDKFLCSTDLCQGQSFISHLINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLB4</accession>
    <name>A0A481QLB4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA-specific 2-thiouridylase MnmA</fullName>
            <ecNumber evidence="7">2.8.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">mnmA</name>
        <name evidence="8" type="ORF">C3B55_00449</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47032"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:11728"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:87170"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.13" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00449"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00144">
        <property type="entry name" value="tRNA_thiouridyl_MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="Adenine_a_hdrlase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="tRNA-specific_2-thiouridylase"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 7" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Interaction with target base in tRNA" evidence="7">
        <location>
            <begin position="103"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with tRNA" evidence="7">
        <location>
            <begin position="154"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with tRNA" evidence="7">
        <location>
            <begin position="316"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="7">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="7">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="7">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="374" mass="41791" checksum="D55774900448D768" modified="2019-06-05" version="1">MPDPAFSDVNKKRVIVAMSGGVDSSVAAVLLVEQGYKVEGLFMKNWEEDDGTKYCTTMNDLADAQAVCDKIGIKLHTANFAAEYWDNVFEHFLTEYQAGRTPNPDIWCNREIKFKVFLKYTMMLGTDLIATGHYVRRREVDGRTELLKGLDPNKDQSYFLHAVGVEQIAKTLFPVGKLNKSTVRKIAEKHGLVTAKKKNSTGICFIGKRRFSDFLKQYLPAQPGKIKTIDGKIIGRHHGLMYHTIGQRHGLGIGGLNDAGKEPWYVISKELQHNTLIVGQGNQHSLLFSSTLLASEIYWINPMDLKTPRRLTATVRYRQSQQPCTISKIASGYRTTFDNPQRAVAPGQSVVFYDGEICLGGGVIEVAEPWSNAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLB5</accession>
    <name>A0A481QLB5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 11">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">rplV</name>
        <name evidence="12" type="ORF">C3B55_00282</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="8 11">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g., L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 10">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00282"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_B">
        <property type="entry name" value="Ribosomal_L22_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_L22/L17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005727">
        <property type="entry name" value="Ribosomal_L22_bac/chlpt-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="PTHR13501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01044">
        <property type="entry name" value="rplV_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8 10" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="110" mass="12005" checksum="C98B0BBE4FD37EAB" modified="2019-06-05" version="1">MDASAKLLSARISAQKARLVANQIRGKKVSEALNMLVFSSKKAAEIIKKVLNSAVANAEHNEGADLDTLKISNVFINEGRSLKRIMPHAKGRADRIVKRSCHITVKVADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLC4</accession>
    <name>A0A481QLC4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SsrA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Small protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">smpB</name>
        <name evidence="4" type="ORF">C3B55_00352</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3">The tmRNA-SmpB complex associates with stalled 70S ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SmpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00352"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070929">
        <property type="term" value="P:trans-translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09294">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00023">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023620">
        <property type="entry name" value="SmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000037">
        <property type="entry name" value="SsrA-bd_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020081">
        <property type="entry name" value="SsrA-bd_prot_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30308">
        <property type="entry name" value="PTHR30308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01668">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74982">
        <property type="entry name" value="SSF74982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00086">
        <property type="entry name" value="smpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01317">
        <property type="entry name" value="SSRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="164" mass="19023" checksum="EC53A8BFD9D07AE6" modified="2019-06-05" version="1">MLNLMAKQTKYPTGTIAQNKKARYDYFIEHRFEAGLVLAGWEVKSLRANKLQLIDSYVQFKDGGAWLLGTHITPLPTASTHVITESMRTRKLLLNARELTRLAAAVQQKGYACICQSWYWSRHLVKCEIALGRGKKEYDKRDTERKQDANRELNRAMRHIGWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLC9</accession>
    <name>A0A481QLC9_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="4">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nrdB</name>
        <name evidence="6" type="ORF">C3B55_00473</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00473"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="PTHR23409"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="479" mass="55075" checksum="60C05F4344125727" modified="2019-06-05" version="1">MQDTYIPYVDLPGYALYIVLLKINSTIYSAHIILISTNYYCAQYFNTIKFAKNFFGHLRRKNLMLNWDEVDSATTDALAIKGTNTGYTSKDSTSHLDNAGISAAWEARNVTDSDSAAVVRAKAALEKLDVDKGLAERESSAARVTVDEKRMINCRADLNQLVPFKYDWAWQKYLDSCANHWMPQEVNMTADIALWKNSTGLTDDERRIVMRNLGFFSTADSLVANNLVLVLYRLITNPECRQYILRQAFEEAIHTHAYQYCIESLSMDEGEIFNMYHEIPSVAKKAAWSLKYTRSISEPKFETGTLKNDKALLRNLIAYYCVLEGIFFYCGFTQILSMGRRNKMTGVAEQFQYILRDESMHLNFGIDVINQIKIENPHLWDAKIKEETTQMILQGTQLEIEYARDTMPRGVLGMNAAIMEDYLKFIANRRLSQIGLKQEYPGTTNPFPWMSEIMDLKKEKNFFETRVIEYQTGGALSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLD6</accession>
    <name>A0A481QLD6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lipid A export ATP-binding/permease protein MsbA</fullName>
            <ecNumber evidence="12">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">msbA</name>
        <name evidence="12" type="ORF">C3B55_00210</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.-" evidence="12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00210"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034040">
        <property type="term" value="F:ATPase-coupled lipid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011917">
        <property type="entry name" value="ABC_transpr_lipidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02203">
        <property type="entry name" value="MsbA_lipidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="31"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="354"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="388"/>
            <end position="395"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX81573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="603" mass="66940" checksum="20120F5600D7F74E" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLI1</accession>
    <name>A0A481QLI1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">rpsM</name>
        <name evidence="8" type="ORF">C3B55_00300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00300"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019980">
        <property type="entry name" value="Ribosomal_S13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="PTHR10871:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03631">
        <property type="entry name" value="uS13_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="118" mass="13440" checksum="B78B3FAA744DA362" modified="2019-06-05" version="1">MARIAGVNIPDNKHTVISLTYIYGIGRTTAQKICAVAGVNYIVQIKDLSDEHIEVLRGEVAKFTTEGDLRREINMKIKRLMDLGCYRGLRHRRGLPVRGQRTKTNARTRKGPRKPIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLI6</accession>
    <name>A0A481QLI6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7 8">Elongation factor Tu</fullName>
            <shortName evidence="7">EF-Tu</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tuf1</name>
        <name evidence="7" type="synonym">tuf</name>
        <name evidence="10" type="ORF">C3B55_00275</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00275"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="10"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="397" mass="43632" checksum="D3E7DDBF11975076" modified="2019-06-05" version="1">MAKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSTVVDFDQIDSAPEEKARGITINTAHVEYNSLIRHYAHVDCPGHADYIKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPHIVVYLNKADLVDDAELLELVEMEVRDLLSAYDFPGDDTPIIIGSARMALEGKDENEMGTTSIRKLVEALDSYIPNPERVIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVQDQLEIIGLRDTAATTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLVKPGSVKPHIKFEAEVYVLNKEEGGRHTPFFKGYRPQFYFRTTDVTGNCELPEGVEMVMPGDNIKMVVTLIKTIAMEDGLRFAIREGGRTIGAGIVSKIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLK1</accession>
    <name>A0A481QLK1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00108"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="49" mass="5763" checksum="0FA454DB727A5744" modified="2019-06-05" version="1">MLFYNISPCRSVISVGWYLLAETSMYQHMMLDILVFLRLQPLNFISLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLM8</accession>
    <name>A0A481QLM8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Methionine aminopeptidase</fullName>
            <shortName evidence="2">MAP</shortName>
            <shortName evidence="2">MetAP</shortName>
            <ecNumber evidence="2 3">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 5" type="primary">map</name>
        <name evidence="5" type="ORF">C3B55_00490</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2 3">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="2 3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00490"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="15"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="261" mass="28617" checksum="419991A74C542C19" modified="2019-06-05" version="1">MTTVTLKTAKDILGMRIAGKLAADVLEMIAEYVKPGITTGALDHICHDYIVNVQQAIPAPLNYKGFPKSICTSINHVVCHGIPSNKLLKDGDTLNIDITVIKDSYHGDTSRMFHVGNVPIWAERLSQIAQECMYKAIKIVKPGCCLGDIGEVIQSHAEKNGFSVVREFCGHGIGKVFHEDPQVLHYGCAGSGMELKAGMTFTIEPMINQGKAGTRVLSDGWTAITKDRKLSAQWEHTLLVTDTGYEIFTLRTDDTISRISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLN1</accession>
    <name>A0A481QLN1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidoglycan D,D-transpeptidase MrdA</fullName>
            <ecNumber evidence="3">3.4.16.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Penicillin-binding protein 2</fullName>
            <shortName evidence="3">PBP-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">spoVD</name>
        <name evidence="3" type="synonym">mrdA</name>
        <name evidence="6" type="ORF">C3B55_00325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes cross-linking of the peptidoglycan cell wall.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transpeptidase family. MrdA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00325"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02081">
        <property type="entry name" value="MrdA_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017790">
        <property type="entry name" value="Penicillin-binding_protein_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03423">
        <property type="entry name" value="pbp2_mrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="3" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_dimer" evidence="5">
        <location>
            <begin position="64"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="4">
        <location>
            <begin position="268"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="615" mass="68979" checksum="367F9350A84F52BA" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLN3</accession>
    <name>A0A481QLN3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein signal peptidase</fullName>
            <ecNumber evidence="6">3.4.23.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prolipoprotein signal peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Signal peptidase II</fullName>
            <shortName evidence="6">SPase II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">lspA</name>
        <name evidence="8" type="ORF">C3B55_00421</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein specifically catalyzes the removal of signal peptides from prolipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.</text>
            <dbReference type="EC" id="3.4.23.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; lipoprotein biosynthesis (signal peptide cleavage).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the peptidase A8 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.36" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00421"/>
    <dbReference type="UniPathway" id="UPA00665"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00161">
        <property type="entry name" value="LspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001872">
        <property type="entry name" value="Peptidase_A8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33695">
        <property type="entry name" value="PTHR33695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01252">
        <property type="entry name" value="Peptidase_A8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00781">
        <property type="entry name" value="LIPOSIGPTASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00077">
        <property type="entry name" value="lspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="183" mass="20827" checksum="507C69F9294994B2" modified="2019-06-05" version="1">MALVKFDIMSNASSRFGCLGWLVLSVLVLVIDHFSKTSVNNTLHMYQRIVVVPDYLSWTLTYNTGAAFSLLADSDGWQRWFFVVITMIVSAVLVVWLKRLGRHDTWLAIALALVLGGAQGNLYDRITIGYVIDFILLHWHNRYYFPAFNFADITITVGAIILLLDIRKRKKTREVVNDSLSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLQ7</accession>
    <name>A0A481QLQ7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endolytic peptidoglycan transglycosylase RlpA</fullName>
            <ecNumber evidence="3">4.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rlpA</name>
        <name evidence="6" type="ORF">C3B55_00328</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Lytic transglycosylase with a strong preference for naked glycan strands that lack stem peptides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the RlpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00328"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008932">
        <property type="term" value="F:lytic endotransglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042834">
        <property type="term" value="F:peptidoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000270">
        <property type="term" value="P:peptidoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02071">
        <property type="entry name" value="RlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034718">
        <property type="entry name" value="RlpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012997">
        <property type="entry name" value="RplA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007730">
        <property type="entry name" value="SPOR-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036680">
        <property type="entry name" value="SPOR-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05036">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110997">
        <property type="entry name" value="SSF110997"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="SSF50685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00413">
        <property type="entry name" value="rlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51724">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPOR" evidence="5">
        <location>
            <begin position="250"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="331" mass="37014" checksum="F488E7D3E862D532" modified="2019-06-05" version="1">MQLLLFQKMLKLVVFILLFLLVVSCSTTRFIQAYKFSDSVFNAQSSQDSNHIQKDGAPWWDVDISKIQDATPTLHSGSYKVSPYTVLGKNYFPLKESKNYVQSGIASWYGTKFNGRNTANGEVYDLYGMSAAHKTLPLPSYVRVTNLDNNRSVILRVNDRGPFYSDRIIDLSYAAAKKLGYAEIGTARVKVESINPIQYLAQSNKLVPLIRNEQQTLQLQVTASADKIEQRTPRFVAFSTVVVPHTMRGALKTHSQYLQVGAFANQDAAKLLRFKLSGIVNAPVFIGPIMRNQQILYCVRIGPISSPSEVVSIQNSIRLISLRQPSIVTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLR6</accession>
    <name>A0A481QLR6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative siderophore transport system ATP-binding protein YusV</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">yusV</name>
        <name evidence="5" type="ORF">C3B55_00613</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00613"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="261" mass="28518" checksum="C82721C9BE6A82EC" modified="2019-06-05" version="1">MSILHAQQLDIGYGATRIVQGLSFALSANRVTALIGPNGCGKSTLLKAFARILKPTHGRLTIYGQTYDSLSARQLARQIAFLPQALPVPEGVSVRQLVACGRSPYNSLWGRLSGNDQSHVTKAIQRLELDSLANRALTDLSGGQRQRAWLAMVLAQNAPVVLLDEPTTYLDISHQVELLDLMGQLVAEGKTVITVLHDINQACRYADYLAVMSCGQLVAYGTPAEVINTELMRQVFDVQVQIMREPFAGTPMCLVEKSTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLS9</accession>
    <name>A0A481QLS9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00622"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="82" mass="8963" checksum="51634C60E79FB1FA" modified="2019-06-05" version="1">MHLMAIMCSVVFRAASINLSDKLLPCVIQVIVISPIIIVYVGSIALQSLSCICAKILYGATPRVVTYLHMLCIKSDIKQLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLT2</accession>
    <name>A0A481QLT2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00635"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="93" mass="10989" checksum="5A497A2E66B78622" modified="2019-06-05" version="1">MRSKTKRKITKVSVDSQDIRLLIISIVTRPLMLWWHKSIAFFKRRNPQAFSLGTNIDIHRVSTEQNTTKTLREIFKYNPIRKNSDALEIAMNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLT5</accession>
    <name>A0A481QLT5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfate adenylyltransferase subunit 2</fullName>
            <ecNumber evidence="2">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">ATP-sulfurylase small subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Sulfate adenylate transferase</fullName>
            <shortName evidence="2">SAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 4" type="primary">cysD</name>
        <name evidence="4" type="ORF">C3B55_00414</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">With CysN forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of CysD, the smaller subunit, and CysN.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the PAPS reductase family. CysD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00414"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00204"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019419">
        <property type="term" value="P:sulfate reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00064">
        <property type="entry name" value="Sulf_adenylyltr_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011784">
        <property type="entry name" value="SO4_adenylTrfase_ssu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002936">
        <property type="entry name" value="CysDAde_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02039">
        <property type="entry name" value="CysD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAPS_reduct" evidence="3">
        <location>
            <begin position="28"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="305" mass="35458" checksum="E0CCFF4030007503" modified="2019-06-05" version="1">MVDKMTHLKQLEAESIHIIREVVAEFDNPVMLYSIGKDSAVMLHLARKSFYPGKLPFPVMHVDTCWKFQDMYKFRDKMVKELDLNLITYVNCEGVAQGISPFTHGSGKHTDIMKTQSLKQAMNKHGFDAAFGGARRDEEKSRAKERVYSFRDKKHRWDPKNQRPELWNIYNGNINNGESIRVFPLSNWTELDIWQYIYLDNIPIVPLYFAAERDVIEKNGMLIMIDDDRILKYLSNNDKARIVKKKVRFRTLGCYPLTGAVESEAETLTDIIHEMLLTRTSERQGRAIDHDGAGSMENKKRQGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLU0</accession>
    <name>A0A481QLU0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Outer membrane protein assembly factor BamD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">bamD</name>
        <name evidence="3" type="ORF">C3B55_00424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BamD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00424"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15830">
        <property type="entry name" value="BamD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00922">
        <property type="entry name" value="OM_assembly_BamD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017689">
        <property type="entry name" value="BamD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039565">
        <property type="entry name" value="BamD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13525">
        <property type="entry name" value="YfiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03302">
        <property type="entry name" value="OM_YfiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="YfiO" evidence="2">
        <location>
            <begin position="32"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="292" mass="33223" checksum="FFAB030CB00F7DDE" modified="2019-06-05" version="1">MQLKHILLVASLTMTSACSSTKTVVDESLREIELYQVVQKDLNNNNYISAIATLKTMESRYPFGRYATQAQLDLIYANYKNAEPEAAKSAADRFIHLHQQHPNVDYAYYMKGLASFNQDIGLLARFLPLDMTKRDPGAARNSYNEFAQLTSRYPNSIYASDAKKHMIYLRNLLASYEIHVAHYYLTRQAYIAAANRGRYVIEKFQETPSVGDGLAVMIEAYQYLHLNALATTSLKTLQLNYPDHPSLQNNKFTSEITKATNRSWLNQHSLSPIESIPPLPPSHTEGYLMEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLV0</accession>
    <name>A0A481QLV0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">[Ribosomal protein S18]-alanine N-acetyltransferase</fullName>
            <ecNumber evidence="5 6">2.3.1.266</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rimI</name>
        <name evidence="8" type="ORF">C3B55_00395</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Acetylates the N-terminal alanine of ribosomal protein S18.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>acetyl-CoA + N-terminal L-alanyl-[ribosomal protein bS18] = CoA + H(+) + N-terminal N(alpha)-acetyl-L-alanyl-[ribosomal protein bS18]</text>
            <dbReference type="Rhea" id="RHEA:43756"/>
            <dbReference type="Rhea" id="RHEA-COMP:10676"/>
            <dbReference type="Rhea" id="RHEA-COMP:10677"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64718"/>
            <dbReference type="ChEBI" id="CHEBI:83683"/>
            <dbReference type="EC" id="2.3.1.266"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the acetyltransferase family. RimI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.266" evidence="5 6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00395"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008999">
        <property type="term" value="F:ribosomal protein S5-alanine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017189">
        <property type="term" value="P:N-terminal peptidyl-alanine acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02210">
        <property type="entry name" value="RimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043690">
        <property type="entry name" value="RimI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="7">
        <location>
            <begin position="5"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Acetyl-CoA binding" evidence="5">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="150" mass="17030" checksum="2722D8A2297DFA06" modified="2019-06-05" version="1">MSEALSFCPLTEVNLDAVLKIEYAAFSFPWNSKIFLSKLDKHQSWLMFKGTQQVGHGVMQIIFDEAHLLNIAIKPENQGCGLGLALLEYLMSSAHASNVRECFLEVRDSNTNALRLYERYGFNRIGYRHDYYPAIGGREDAVVMAYTIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLZ1</accession>
    <name>A0A481QLZ1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lipopolysaccharide export system protein LptA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">lptA</name>
        <name evidence="5" type="ORF">C3B55_00523</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. May form a bridge between the inner membrane and the outer membrane, via interactions with LptC and LptD, thereby facilitating LPS transfer across the periplasm.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the lipopolysaccharide transport and assembly complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LptA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00523"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015920">
        <property type="term" value="P:lipopolysaccharide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01914">
        <property type="entry name" value="LPS_assembly_LptA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014340">
        <property type="entry name" value="LptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005653">
        <property type="entry name" value="OstA-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03968">
        <property type="entry name" value="LptD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03002">
        <property type="entry name" value="outer_YhbN_LptA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0574">Periplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019873717" description="Lipopolysaccharide export system protein LptA" evidence="3">
        <location>
            <begin position="25"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="OstA-like_N" evidence="4">
        <location>
            <begin position="34"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="191" mass="20691" checksum="BC88B7A8FE50C280" modified="2019-06-05" version="1" precursor="true">MKFVNILHILLGLCAALESVIICALPSDSQQSIYIQADNAQLDNKQGVATYTGAVIITQGSMKITGNTVTFTRTMAGEIDVVTAVGNLAYFEQQQSASDQTPMKGYGRIIQYLAQQNRIILIDQARVVSSDGNTTDSEKITYDTVKQIAQAGRATGIKISTPRPQIDMVIQSKQKDGVSLHALKPDTNKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLZ5</accession>
    <name>A0A481QLZ5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00627"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="38" mass="4525" checksum="DDC50A8AC63B7828" modified="2019-06-05" version="1">MADLFFVDLLLLYIIVNVVNYIIHLEAEGKHYKEIFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM10</accession>
    <name>A0A481QM10_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycerol-3-phosphate dehydrogenase [NAD(P)+]</fullName>
            <ecNumber evidence="7">1.1.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">NAD(P)H-dependent glycerol-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 16" type="primary">gpsA</name>
        <name evidence="16" type="ORF">C3B55_00651</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:11096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7 13">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 11">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.94" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00651"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004367">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00394">
        <property type="entry name" value="NAD_Glyc3P_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10 11" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 7 13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_Gly3P_dh_N" evidence="14">
        <location>
            <begin position="7"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_Gly3P_dh_C" evidence="15">
        <location>
            <begin position="180"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 9">
        <location>
            <begin position="255"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX81976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="341" mass="36546" checksum="C0AC1777A54B209C" modified="2019-06-05" version="1">MTEQRPIAVLGGGSFGTAVANLLAKNGQAVRQWMRDPEQAAAIRIYRENPRYLKGIKIHPAVEPVTDLLVTLTDCNLCFIALPSSALRLVLAPHAEYLVGKQLVSLTKGIEVHTFKLMSEILKEIAPQAGIGVLSGPSLAREVAENTLTATVVASENEELCKCVQTALHGHTFRVYTSSDRFGVELGGALKNVYAIIAGMAVALGMGENTKSMLITRILAEMTRFAVNQGAKPMTFLGLAGVGDLIVTCSSPKSRNYQVGFALGQGLSLEDAVMRLGEVAEGVNTLRVLKAKAQEVGVYMPLVAGLHAILFEGHTLIKVIELLMRAEPKTDIDFTSTSGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM13</accession>
    <name>A0A481QM13_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carboxynorspermidine synthase</fullName>
            <ecNumber evidence="3">1.5.1.43</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.5.1.43" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00504"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sacchrp_dh_NADP" evidence="1">
        <location>
            <begin position="5"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Sacchrp_dh_C" evidence="2">
        <location>
            <begin position="150"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="414" mass="45521" checksum="6DE880D899BE4CA0" modified="2019-06-05" version="1">MKKNVLIIGAGGVAKVVAHKCAQHNDELGRIAIASRNISKCQLIIDSVKAKGSFKVPADIQVFALNALNVEATKVLIRETESQIVINVGSAFLNMSVLRACINTGVAYLDTAIHEEPSKVCETPPWYGNNEWNHLEECKQKNITAILGVGFDPGVVNAYAALAQQQYFDRIDSIDIFDVNAGSHGKYFATNFDPEINFREFTGQVWSWQNSQWTSNAMFEIKRTAALPVVGSQNLYLTGHDEVHSLSKNLNVPNVRFWMSFGEHYINVFTVLKNLGLLSEKPVKTAEGMEVVPLKVVKAVLPDPSSLAPSYTGKTCIGDLVKGTKDGLPRERFIYNVACHEKAFAETDSQSISYTAGVPPVAAALLVARGEWDVQRMANVEELPAEPFLKALDVMGLPTRIKDENGDRAWDLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM22</accession>
    <name>A0A481QM22_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable cytosol aminopeptidase</fullName>
            <ecNumber evidence="8">3.4.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucine aminopeptidase</fullName>
            <shortName evidence="8">LAP</shortName>
            <ecNumber evidence="8">3.4.11.10</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Leucyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">pepA</name>
        <name evidence="10" type="ORF">C3B55_00562</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.</text>
            <dbReference type="EC" id="3.4.11.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.1" evidence="8"/>
    <dbReference type="EC" id="3.4.11.10" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00562"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00181">
        <property type="entry name" value="Cytosol_peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023042">
        <property type="entry name" value="Peptidase_M17_leu_NH2_pept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="PTHR11963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTOSOL_AP" evidence="9">
        <location>
            <begin position="354"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="504" mass="53941" checksum="C880555E24E8D286" modified="2019-06-05" version="1">MIILRDSKMELVVKSVSPETLKTATLVVSVGEWRKIGIATKKLDALSGGAIGAILKRGDLAGKVGQSLLLQSLPNLKADRVLLVGVGRDAELDDRPFRKIVSTILATLKSLGGTDAVLALDEIIVKGRDSYGKTRLLVESLIDGGYIFDQFKSQKTEPRVLQKITLLTMKITQAEVERAVTHATAIANGMSFARDLGNLPPNVCHPIYLGEQAKALGKKFKDLKIEVISEKRIKELGMGAFYAVGQGSNQQSRLIVMKYSGSIKSEKPYVLVGKGITFDTGGISLKPSASMDEMKYDMGGAASIFGALHAVLELKLPINLVCILACAENMPSGNATRPGDIVTTMSGQTVEILNTDAEGRLVLCDALTYAECFNPRAVIDIATLTGACVVALGSHTSGLLGNNGELIEQLLSAGMAADDRVWQLPMFDEYQEQLDSPFADIANVGNSKAGTITAACFLSRFAKNFHWAHLDIAGTAWTSSGKDKGATGRPVPLITQYLIDRAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM28</accession>
    <name>A0A481QM28_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00762"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="42" mass="4693" checksum="9CB37850D3F94C75" modified="2019-06-05" version="1">MVSNNFGFVQFLEPELAVTSLLLECKLQMLALALLYFVSADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM31</accession>
    <name>A0A481QM31_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cell division protein FtsQ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">ftsQ</name>
        <name evidence="10" type="ORF">C3B55_00574</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential cell division protein. May link together the upstream cell division proteins, which are predominantly cytoplasmic, with the downstream cell division proteins, which are predominantly periplasmic. May control correct divisome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of a complex composed of FtsB, FtsL and FtsQ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">Localizes to the division septum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FtsQ/DivIB family. FtsQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00574"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090529">
        <property type="term" value="P:cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00911">
        <property type="entry name" value="FtsQ_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005548">
        <property type="entry name" value="Cell_div_FtsQ/DivIB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026579">
        <property type="entry name" value="FtsQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045335">
        <property type="entry name" value="FtsQ_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013685">
        <property type="entry name" value="POTRA_FtsQ_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35851">
        <property type="entry name" value="PTHR35851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03799">
        <property type="entry name" value="FtsQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08478">
        <property type="entry name" value="POTRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 8 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="9">
        <location>
            <begin position="73"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="289" mass="32864" checksum="AD25A3D0BC5FB30F" modified="2019-06-05" version="1">MNCVSLHHQQSPVHSFKQMPRGASRIVAKEPMSVRFSKAKLAFLKALFWPVLLVAFSFCTYEGAQRLLLYIDWPITRISVHGNLSHICQQTVQQRIGQYITTSFFTIDLAGIRVELKRIPWVAHAEVYRIWPDQVVICLEEQLPVASWGDSMLLNNHGQAFVPCELTNYKHLPHLFGPQLAQQQVIQQYQALSQILQPLGFSIARLELRERGSWFLTTAASSYGPGIELLLGRDRLVEKMRRFIVIYNKTLKKQIINIASVDLRYANGLAVGWREPSASTIAKPAIAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM36</accession>
    <name>A0A481QM36_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ferredoxin--NADP(+) reductase</fullName>
            <ecNumber evidence="4">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fpr</name>
        <name evidence="7" type="ORF">C3B55_00772</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + NADP(+) + 2 reduced [2Fe-2S]-[ferredoxin] = NADPH + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferredoxin--NADP reductase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00772"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06195">
        <property type="entry name" value="FNR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033892">
        <property type="entry name" value="FNR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="2"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="259" mass="29671" checksum="F4D22B8784E047E3" modified="2019-06-05" version="1">MNNVNHEHVLSVHHWNDTLFSFKCTRDPGLRFENGQFVMIGLQQPNGYPLMRAYSIASPNWESYLEFLSIKVPDGPLTSQLQHLKEGDIIIISKKPTGTLVLDDLKPGKHLYLLGTGTGLAPFMSVIQDPETYERFEKVILCHGVRYINEVAYYEFITEHLPQNEFFGEALRDKLIYYPTVTREPFKNEGRLTDLMRSGKLFSDISQPPINHENDRAMLCGSPAMLNETSEVLNNFGLKVSLRMREPGDYVIERAFVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM40</accession>
    <name>A0A481QM40_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidoglycan D,D-transpeptidase FtsI</fullName>
            <ecNumber evidence="7">3.4.16.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Penicillin-binding protein 3</fullName>
            <shortName evidence="7">PBP-3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">ftsI</name>
        <name evidence="10" type="ORF">C3B55_00582</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes cross-linking of the peptidoglycan cell wall at the division septum.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transpeptidase family. FtsI subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00582"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02080">
        <property type="entry name" value="FtsI_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037532">
        <property type="entry name" value="FtsI_transpept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0717">Septation</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_dimer" evidence="9">
        <location>
            <begin position="56"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="8">
        <location>
            <begin position="245"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="581" mass="64023" checksum="8C0B16D922E6837F" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM41</accession>
    <name>A0A481QM41_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00387</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00387"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="61" mass="7298" checksum="8C678602F2B06F62" modified="2019-06-05" version="1">MCVLLLFDKIYTNLYHSLSLFNLIFMRLVLKKNNPYIFYLKELQQKNRGFFVLAIRGGLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM49</accession>
    <name>A0A481QM49_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UvrABC system protein C</fullName>
            <shortName evidence="7">Protein UvrC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Excinuclease ABC subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">uvrC</name>
        <name evidence="11" type="ORF">C3B55_00593</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with UvrB in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the UvrC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00593"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00203">
        <property type="entry name" value="UvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004791">
        <property type="entry name" value="UvrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038476">
        <property type="entry name" value="UvrC_RNase_H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08459">
        <property type="entry name" value="UvrC_HhH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00194">
        <property type="entry name" value="uvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 7" id="KW-0228">DNA excision</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 7" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="GIY-YIG" evidence="9">
        <location>
            <begin position="16"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="8">
        <location>
            <begin position="203"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="UVRC" evidence="10">
        <location>
            <begin position="253"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="607" mass="67788" checksum="C44C7FFA9A31B0F2" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM52</accession>
    <name>A0A481QM52_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="11">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">cysS</name>
        <name evidence="13" type="ORF">C3B55_00595</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00595"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="PTHR10890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DALR_2" evidence="12">
        <location>
            <begin position="342"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="267"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="464" mass="52588" checksum="C1B76EC359EC0FDA" modified="2019-06-05" version="1">MLTIYNTLSQTKEVFQPLNGNKVRMYVCGITVYDYCHIGHGRSMVTFDLVTRWLRFSGYDLTYVRNITDIEDKIINRAKENCEPFHVLTERMIIAMHEDEARLNILKPDTEPRATDYISSILRMIQTLIDKGYAYCAPGNSGVYYRIAKFIGYGRLSRKKIEDLRIYTRVDVDDAKQDQLDFVLWKAAKPGEPSWESPWGAGRPGWHIECSVMSTCCLGETFDIHGGGSDLKFPHHENEIAQSEAATGKTYANTWMHCGMIRINGEKMSKSSHNFVVIRDVLKKYHPEVVRYLLISSHYRSVVNYSEDSLKDAKGALERLYHSLKGLQGGVPPTGGEVFVARFTEVMNNDFSTPEACAILFKMAREINRLRESNLNAASGLAARLKELASVLGILQMEAVDFLQASAARCVDTVEVYTLIQARLVARANKDWAESDHIRKQLNSMGVLLEDGKSETLWRLTDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM60</accession>
    <name>A0A481QM60_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="6">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">GTP cyclohydrolase I</fullName>
            <shortName evidence="6">GTP-CH-I</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">folE</name>
        <name evidence="8" type="ORF">C3B55_00820</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homopolymer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00820"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035998">
        <property type="term" value="P:7,8-dihydroneopterin 3'-triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="PTHR11109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydroI" evidence="7">
        <location>
            <begin position="4"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="181" mass="20201" checksum="1A4DF168C3B3637C" modified="2019-06-05" version="1">MFLEQNYTAILDQLGEDVSREGLLDTPKRAAKAMQYLCRGYEQTLEEVTNGALFSSDNSEIVLVKDIELYSLCEHHLLPFIGKAHVAYIPSGKVLGLSKVARIVDMYARRLQIQENLSRQIANAVMQVTGALGVAVVIEAKHMCMMMRGVEKQNSSMITSVMLGEFRENAATRSEFLSLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM61</accession>
    <name>A0A481QM61_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tyrB</name>
        <name evidence="6" type="ORF">C3B55_00808</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00808"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="PTHR11879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="5">
        <location>
            <begin position="29"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="403" mass="44168" checksum="A7B863A195C20410" modified="2019-06-05" version="1">MSLFSAVKMAPRDPILGLNEAFNTDTRTTKVNLGVGVYCNEEGKIPLLRAVAKAEAIRAAQHATRGYLPIDGILAYGQAVQKLLFSADSPPLSAGRVITAQTIGGTGALKIGSDFLKQILPNAAVAISDPSWENHQALFKTAGFLVRTYRYYDAVTHDINRTGLLEDLNSLPPKSIVVLHACCHNPTGVDLSYTDWQKVLDVIRDKNLVPFLDLAYQGLGENIYEDAATVRMFAESGLTFLVSSSFSKSFSLYGERVGALSIASESKEESARILSQIKRVIRTNYSNPPTHGATIVATVLNNLKLRAQWEAELAEMRSRINSMREQMVTKLAGAALDYDFSFVGRQRGMFSYSGLTVKQVTRLRTEFGVYALDNGRICMAALNQSNINTVTKAIVQVLKTCTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM78</accession>
    <name>A0A481QM78_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inner membrane protein YbjJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ybjJ</name>
        <name evidence="7" type="ORF">C3B55_00628</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00628"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="16"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="383" mass="39291" checksum="A0EE6D590C95CC8E" modified="2019-06-05" version="1">MTIIPTTALVVPGRPEQISTRIAFFIAGFGIATWAPLVPYAKARTNINDGKLGLLLLCIGIGSIIAMPAASVLTSRYGCRRVLTIGTIMICLALPMLATVSSIPLLMTALLLFGAGLGTVDLTSNLQAVLVERTSGKTMMSGFHGLFSLGGIVGAAGVAGLLGLGLLPWQATLVVITIIATALLKAIPHLLPYGSKISGPAFSMPHGVVLFIGCLCFIVFMVEGAILDWSAVFLSAERSLDGAYAGLGYAAFALTMTAGRLTGDAIVRRLGASRVIVVGCVLATGGILLATFFPAWKTALPGYALVGAGCSNIVPVLYTAVSKQTVMPEHIAVPAITTLGYAGILAGPTIIGFIAHGSSLSIAFVLIALLLAGVAISGKILKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM96</accession>
    <name>A0A481QM96_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ppnK</name>
        <name evidence="5" type="synonym">nadK</name>
        <name evidence="6" type="ORF">C3B55_00470</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00470"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="72"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="146"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="187"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="296" mass="32398" checksum="8D234AFB2C58B713" modified="2019-06-05" version="1">MEQFRNIGIIGRLGSIQVLNTVHRLKKFLLTRHLCVILEDNIAEILPGHGLQTSSRKMLGEVCDMVIVVGGDGSLLDAARALVQHNVPVLGINRGSLGFLTDILPNDLEVEVAKVLDGYYLVGNRFLLQVEVCRYGEAIGHGDALNDVVLHPGKSTRMIEFELYIDEQFVCSQKADGLIVATPTGSTAYALSAGGPIMHPKLDAIVIVPMYPHMLSSRPIVVDGNSELKIVVSKDMHIYPQTSCDGQNHFSCAPGDTITVSKKAQQLQLIHPLDHNYYEVCRTKLGWGSSLESKCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMB2</accession>
    <name>A0A481QMB2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00629"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="40" mass="4550" checksum="7FC9394CC473E937" modified="2019-06-05" version="1">MMPIKYSFFIFFVEVFTSAIELLIAALIQSIFQIGLSFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMB7</accession>
    <name>A0A481QMB7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">yadH</name>
        <name evidence="9" type="ORF">C3B55_00643</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00643"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="8">
        <location>
            <begin position="11"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="258" mass="28682" checksum="45EEF80F694D3DE5" modified="2019-06-05" version="1">MAELQFNMVALQTIVYREVKRFTRIWPQTLLPPAITMVLYFVIFGNLIGRQIGNIGGFTYMEYIVPGLIMMSVITNSYSNVVSSFFGSKFQRSIEELMVSPVSPHIILIGYALGGMLRGLMVGIIVTLLSLFFTHLQVHHLGIILVVIVLTVTIFSLLGLLNAVFARNFDDISIIPTFVLTPLTYLGGVFYSITLLPPFWQTVSLANPVLHMVNAFRYGILGVSDIKINVAITFMLVAILVLYISCARLLVSGRGLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMD2</accession>
    <name>A0A481QMD2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thiol:disulfide interchange protein DsbE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dsbE</name>
        <name evidence="7" type="ORF">C3B55_00671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Periplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thioredoxin family. DsbE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00671"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03010">
        <property type="entry name" value="TlpA_like_DsbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004799">
        <property type="entry name" value="Periplasmic_diS_OxRdtase_DsbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00385">
        <property type="entry name" value="dsbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="34"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="178" mass="20152" checksum="C41F384456CFBE1F" modified="2019-06-05" version="1">MKRWLMVLPLVVFLVVVALLCRGLYLDQSYLPSAIIGKKLPDFSLKTVQGNQTITQTDLLGKPALVNVWATWCTSCRAEHLVLNKLAENGVVIYGINYKDNNVEALKWLTEFHDPYQLDIRDEDGTLCLDFGVYGAPETFFIDAKGVIRDKYIGVINEVVWREQLVAKYQSLVDEATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QME0</accession>
    <name>A0A481QME0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dephospho-CoA kinase</fullName>
            <ecNumber evidence="2">2.7.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Dephosphocoenzyme A kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">coaE</name>
        <name evidence="3" type="ORF">C3B55_00430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00430"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00152">
        <property type="entry name" value="TIGR00152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="18"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="205" mass="22991" checksum="E8F2D339542CBDD8" modified="2019-06-05" version="1">MTISIVIPWILGLTGGIGSGKSAAAQHFLSLGIDHVDADHAARWVVKPGQPALTSIAAYFGDDVLLPDGQLNRAALRTLIFGAPEKRRWLEALLHPLISEEIRSHLARAQSPYVILVSPLLIELGQYKMTQRILVIDAPQSLQIQRTMQRDGASEQQVHEILQAQSSRKDRLNHADDVLVNDQDLSWLHGEVERLHYFYLTLRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QME4</accession>
    <name>A0A481QME4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Heme exporter protein C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytochrome c-type biogenesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">ccmC</name>
        <name evidence="10" type="ORF">C3B55_00674</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CcmC/CycZ/HelC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00674"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003557">
        <property type="entry name" value="Cyt_c_biogenesis_CcmC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045062">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA/CcmC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071">
        <property type="entry name" value="PTHR30071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01386">
        <property type="entry name" value="CCMCBIOGNSIS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01191">
        <property type="entry name" value="ccmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="9">
        <location>
            <begin position="28"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="251" mass="27945" checksum="01842F9D8ACF6B91" modified="2019-06-05" version="1">MNWTWFRKLGSPKLFYGISSQLLPWLSAAAVLLIGIGLVWGLAFAPLDYQQGNSFRIIYIHVPTAMLAQSCYLMLAACGIVSLVWKIKLADVALQCAAPIGAWMTAVALVTGAIWGKPTWGSWWVWDARLTSMLILLFLFFGLIALDNAISNRDSSAKACAVLAIVGVINIPIIKYSVEWWNTLHQGATFTFTKKPAMPLEMWAPLLLVVLGFYCFFSAMLLIRMRLEILKREARTSWVNSAGLASLRARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMG2</accession>
    <name>A0A481QMG2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="1">1.1.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Oxalosuccinate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">icd</name>
        <name evidence="6" type="ORF">C3B55_00451</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the monomeric-type IDH family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00451"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004436">
        <property type="entry name" value="Isocitrate_DH_NADP_mono"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36999">
        <property type="entry name" value="PTHR36999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03971">
        <property type="entry name" value="IDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009407">
        <property type="entry name" value="IDH_monmr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00178">
        <property type="entry name" value="monomer_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 5" id="KW-0521">NADP</keyword>
    <keyword evidence="1 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="133"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="83"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="548"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="585"/>
            <end position="586"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="590"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="601"/>
            <end position="603"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="650"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="2">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="2">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR009407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR009407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR009407-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR009407-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="742" mass="82371" checksum="15C9831C68836ADD" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMH7</accession>
    <name>A0A481QMH7_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Fatty acid oxidation complex subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">fadB</name>
        <name evidence="14" type="ORF">C3B55_00716</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:22432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00716"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036125">
        <property type="term" value="C:fatty acid beta-oxidation multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008692">
        <property type="term" value="F:3-hydroxybutyryl-CoA epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012799">
        <property type="entry name" value="FadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02437">
        <property type="entry name" value="FadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="13">
        <location>
            <begin position="318"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="12">
        <location>
            <begin position="497"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX82038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="718" mass="78065" checksum="CD082594A0BE693E" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMI7</accession>
    <name>A0A481QMI7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis O-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-polyprenyl-6-hydroxyphenol methylase</fullName>
            <ecNumber evidence="1">2.1.1.222</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">3-demethylubiquinone 3-O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.64</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">ubiG</name>
        <name evidence="2" type="ORF">C3B55_00700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3-(all-trans-polyprenyl)benzene-1,2-diol + S-adenosyl-L-methionine = a 2-methoxy-6-(all-trans-polyprenyl)phenol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:31411"/>
            <dbReference type="Rhea" id="RHEA-COMP:9550"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:62729"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="EC" id="2.1.1.222"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.222" evidence="1"/>
    <dbReference type="EC" id="2.1.1.64" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00700"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102208">
        <property type="term" value="F:2-polyprenyl-6-hydroxyphenol methylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008689">
        <property type="term" value="F:3-demethylubiquinone-9 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="230" mass="26010" checksum="362FB3EAE63E07A5" modified="2019-06-05" version="1">MSNVDYTEIAKFEVLAHRWWDCDSEFKLLHDINPLRVNWIDERVNLAGKRVLDVGCGGGILSEAMAQRGATVTGIDMGKAPLAVAKLHQLESRVSIEYYQITAEAMAEEMHEQFDVVTCFEMLEHVPNPSSVISACFRMIKPGGQVFFSTINRNLKAYLFAIIGAEYIMQLLPRGTHNFKKFIRPSELSAWSRQAGLIVKDIIGLTYNPLTKHYKLANDVGVNYMLQTMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMJ0</accession>
    <name>A0A481QMJ0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Lipid-A-disaccharide synthase</fullName>
            <ecNumber evidence="3 11">2.4.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">lpxB</name>
        <name evidence="12" type="ORF">C3B55_00758</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a lipid A disaccharide + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:67828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:137748"/>
            <dbReference type="ChEBI" id="CHEBI:176338"/>
            <dbReference type="ChEBI" id="CHEBI:176343"/>
            <dbReference type="EC" id="2.4.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the LpxB family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.182" evidence="3 11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00758"/>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008915">
        <property type="term" value="F:lipid-A-disaccharide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00392">
        <property type="entry name" value="LpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003835">
        <property type="entry name" value="Glyco_trans_19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30372">
        <property type="entry name" value="PTHR30372"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02684">
        <property type="entry name" value="LpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00215">
        <property type="entry name" value="lpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="375" mass="41353" checksum="348149BE27745338" modified="2019-06-05" version="1">MANLCIALVAGEVSGDILGAGLMRALKAHYPVVRFIGVGGLLMQAEGLMSYFPMERLSVLGLLEVLSRLRELRARRKLLIQTLIREKPDVFVGIDAPDFTLNIELKLRQAGIKTVHYVSPSVWAWRQNRVLKIRDCCDLMLILLPFEARFYEEKGVRVRFVGHTLADTIPLHVNGAAARAELGLPSGPLVALLPGSRGSEIKRLVSIFFDAAKCLQSMKPNVRFVLPCASPLCRAQIEMLLEGCNLPLTLLDSKSHLALAACDAALITSGTATLEALLYKRPMVVVYRLAPLTFWILKRMVKSPYISLPNLLAQRLLVPELLQDDATPEALAQTLLPLIDNGEEQIGSFKDIHHTLRRDASNQAADAVLTLIGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMJ8</accession>
    <name>A0A481QMJ8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Soluble pyridine nucleotide transhydrogenase</fullName>
            <shortName evidence="11">STH</shortName>
            <ecNumber evidence="4 11">1.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">NAD(P)(+) transhydrogenase [B-specific]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 15" type="primary">sthA</name>
        <name evidence="15" type="ORF">C3B55_00711</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + NADPH = NADH + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:11692"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11 12">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.1.1" evidence="4 11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00711"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003957">
        <property type="term" value="F:NAD(P)+ transhydrogenase (B-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00247">
        <property type="entry name" value="SthA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022962">
        <property type="entry name" value="STH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12" id="KW-0274">FAD</keyword>
    <keyword evidence="11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 11 12" id="KW-0520">NAD</keyword>
    <keyword evidence="7 11" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="14">
        <location>
            <begin position="6"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="13">
        <location>
            <begin position="344"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="182"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX82033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="464" mass="51144" checksum="E7E87A8DF3601817" modified="2019-06-05" version="1">MAVYNYDVVVIGSGPAGEGAAMNAAKAGCKIAMVDSRCQVGGNCTHLGTIPSKALRHSVRQIMQFNTSQMFRAIGKPRWFSFPDVLKNAETVISKQVASRTSYYARNRIDLFFGTGSFVDEQTVEVACANGLMEKLVAKHIVIATGSHPYRPADIQFHHSRIYDSDTILNLSHTPRKLIIYGAGVIGCEYASIFSGLGVLVELVDGRDQLLNFLDSEISQALSYHFSNSNIIMRHNEKYERVECLDHGVILYLKSGKKIKADALLWCNGRAGNTDKLCIENIGIKANSRGQIEVDKNYRTCIPNIYGAGDVIGWPSLASAANDQGRSAAGSIVDNGSWRYVNDVPTGIYTIPEISSIGKNEHELTNAKVPYEVGRAFFKSMARSQIAGEPQGMLKILFHRETLEILGVHCFGYQASEIVHIGQAIMNQPGKQNTLKYFVNTTFNYPTMAEAYRVAAYDGLNRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMJ9</accession>
    <name>A0A481QMJ9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00846"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="97" mass="11353" checksum="4026D371E6D108C9" modified="2019-06-05" version="1">MNAYLLLPHSEKFNILFIFTLYQSAKLILHNIECDKRIKSFDSLCFEKVIITQQVIYHYITDQHLHQTNKIEMIFGVAMLELTSKQLLAKKSYCGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMK7</accession>
    <name>A0A481QMK7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase</fullName>
            <shortName evidence="1">MECDP-synthase</shortName>
            <shortName evidence="1">MECPP-synthase</shortName>
            <shortName evidence="1">MECPS</shortName>
            <ecNumber evidence="1 2">4.6.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 4" type="primary">ispF</name>
        <name evidence="4" type="ORF">C3B55_00749</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP</text>
            <dbReference type="Rhea" id="RHEA:23864"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="4.6.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the IspF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.12" evidence="1 2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00749"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00095"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008685">
        <property type="term" value="F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00554">
        <property type="entry name" value="MECDP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00107">
        <property type="entry name" value="IspF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003526">
        <property type="entry name" value="MECDP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036571">
        <property type="entry name" value="MECDP_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43181">
        <property type="entry name" value="PTHR43181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02542">
        <property type="entry name" value="YgbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69765">
        <property type="entry name" value="SSF69765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00151">
        <property type="entry name" value="ispF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="1 2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YgbB" evidence="3">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="1">
        <location>
            <begin position="56"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="1">
        <location>
            <begin position="61"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="1">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="158" mass="16984" checksum="4B32DB4D4BA64F33" modified="2019-06-05" version="1">MRIGHGYDVHCFVEGDFITVGGVRIAHHHGLLACSDGDIMLHALSDALLGAAALGDIGKYFPDTDLTCKGLDSRVLLRHVVDLIHANGWKVGNVDNTIVAQAPQIAQHIESMRILLAADLQTELHQVNVKATTTEKLGFTGREEGIAAHSVVLLLHAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMM1</accession>
    <name>A0A481QMM1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">C3B55_00520</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00520"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030296">
        <property type="entry name" value="MlaF/Mkl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43023:SF6">
        <property type="entry name" value="PTHR43023:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="9"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="269" mass="29418" checksum="169F93B5133E1635" modified="2019-06-05" version="1">MSVNNAYAVELKGLTFKRSSRSIFNNVEIRIPRGKVTGIMGPSGCGKTTLLCLMSMQLCPSAGEVWVNGQNLLTLSRSDLFNARKHIGVLFQSGALFTDLDVFENVAFPLRVHTRLSDEMIRDIVLLKLHAVGLRGTIDLMPEELSGGMKRRVALARAIALDPQILMYDEPFVGQDPIAMGVLVRLIRLLSDTLGITSIIVSHDLAETASIADYLYVVGNGLVLGHGTPEELMNADNPHIRQFMTGDPDGPVPFHFPAADYCSDLLGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMN4</accession>
    <name>A0A481QMN4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nicotinamide-nucleotide amidohydrolase PncC</fullName>
            <ecNumber evidence="2">3.5.1.42</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pncC</name>
        <name evidence="2" type="ORF">C3B55_00777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.42" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00777"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019159">
        <property type="term" value="F:nicotinamide-nucleotide amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="SSF142433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00199">
        <property type="entry name" value="PncC_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="164" mass="17608" checksum="5D53A42C4608B10A" modified="2019-06-05" version="1">MKEITQLATKLGRCLQVLNAHVTIAESCTGGGITEAITRIPGSSAWFETSYITYSNREKTRQLGVSETLFTKGSAVNQEVVESMVRGAQKKSLSRFVVAVSGIAGPDGGLPDTPVGTVWLAFSVSNKVTTKLAYFTGNRNEVRRQTVMAALNGLLRRAAAEIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMP3</accession>
    <name>A0A481QMP3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="14">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="14">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 19" type="primary">pheT</name>
        <name evidence="19" type="ORF">C3B55_00787</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="14"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00787"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02796">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00283">
        <property type="entry name" value="Phe_tRNA_synth_beta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004532">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033714">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00472">
        <property type="entry name" value="pheT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="16">
        <location>
            <begin position="39"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="18">
        <location>
            <begin position="400"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="17">
        <location>
            <begin position="698"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="QAX82105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="792" mass="87885" checksum="08B25E20ABB2D990" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMR3</accession>
    <name>A0A481QMR3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Succinate dehydrogenase hydrophobic membrane anchor subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">sdhD</name>
        <name evidence="17" type="ORF">C3B55_00664</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Membrane-anchoring subunit of succinate dehydrogenase (SDH).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="15">The heme is bound between the two transmembrane subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, plus two membrane-anchoring proteins, SdhC and SdhD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell inner membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00664"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014312">
        <property type="entry name" value="Succ_DH_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38689">
        <property type="entry name" value="PTHR38689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000169">
        <property type="entry name" value="SDH_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02968">
        <property type="entry name" value="succ_dehyd_anc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 15" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 13" id="KW-0813">Transport</keyword>
    <keyword evidence="13" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <note>ligand shared with second transmembrane subunit</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000169-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000169-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX81989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="122" mass="13605" checksum="97D3871DF94F5E42" modified="2019-06-05" version="1">MVTSITNLSRSGLYDWMAQRASAVVLAVYFIFLTVYLVANPGIGYEQWHGLFSHNSMRIFSLLALVALSAHAWIGMWTIATDYLTPMALGKHATAIRFLFQAVCGFAMFAYFVCGVQILWGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMR6</accession>
    <name>A0A481QMR6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">D,D-heptose 1,7-bisphosphate phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">C3B55_01014</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the gmhB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01014"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004446">
        <property type="entry name" value="Heptose_bisP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006543">
        <property type="entry name" value="Histidinol-phos"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42891">
        <property type="entry name" value="PTHR42891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004682">
        <property type="entry name" value="GmhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01656">
        <property type="entry name" value="Histidinol-ppas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Stabilizes the phosphoryl group" evidence="7">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to substrate recognition" evidence="7">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes the phosphoryl group" evidence="7">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR004682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004682-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004682-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR004682-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="177" mass="19273" checksum="6BD39003914C0F18" modified="2019-06-05" version="1">MKLLIIDRDGVINYDSKLYIKSVEEWIPLPGSIEAIAQLNNAGWTVAIATNQSGIARGYYNIATMNAIHAYLRALVAKLGSEIGLIVYCPHKPNQGCNCRKPKPGMLKTITEYYKLPLAGLWFVGDSLDDLEAAQAVDCQPVLVTTGKGKKTQAKNIPVGTLIFEDLAAVAEQLVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMV6</accession>
    <name>A0A481QMV6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fe(3+) dicitrate transport system permease protein FecC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fecC</name>
        <name evidence="8" type="ORF">C3B55_00615</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00615"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="329" mass="34094" checksum="8BA856751B0CD1E8" modified="2019-06-05" version="1">MQRSFAAAGILLLGAILFWFSLYSWSPFVITPTDAWNGLVHQGVAAGNMAYIVTLLRVPRAVCAALVGACLGLAGTLMQGITRNRMASPSLLGVTAGAALGLALFSTGLVVPPFAGGTLLMTCLGGALAWVTVFSLSRAWSPTTAQGRLVLAGMAVAALCVALTRLTVILVEVQAQSVLNWLAGSLANVNTAHVQLLWPCTLIGGLWALWCAPRLNLINLGDDTARSLGVSIGSLRLQVFIASLLLVGASVCVVGPIGFVGLIAPNIIRQFLGNDYCWLIPLSAALGAVIVLGADLLSRAMAFPAETSAGVVTALIGAPFFLFLARRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMV9</accession>
    <name>A0A481QMV9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00829"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="86" mass="9811" checksum="C32E7DA41CDD997C" modified="2019-06-05" version="1">MLAVRYGWYVDICEVCYFSCSGSMVVLNGLWYMAKLDLRLLTKVVTADQAYHWCSACFLVLVFRYRYVANQIAYSLITSLVGFAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMY0</accession>
    <name>A0A481QMY0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00914</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00914"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="46" mass="5250" checksum="D32F38609E5C8721" modified="2019-06-05" version="1">MLLFYVSLVVHFTVLDRQEFIGKAIVAANQDLATSIKMVANNYYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMZ0</accession>
    <name>A0A481QMZ0_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphorylated carbohydrates phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.-" evidence="1"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00863"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="229" mass="25254" checksum="207823928CE2465D" modified="2019-06-05" version="1">MNAPHIIVNPIKAVIFDMDGLLLDTEGIYTEVIQIIAQRYGRTYDWSIKQHIIGCGAQDLAKYIVKALDLPITPDEFLVIREPLMSERFSNTVSMPGAEELVRHLKAHNILIAVGTSSSRNLFNHKTALHREWFGLFDTIVAADDQEISAAKPAPDIFLAAAHRLSVAPEDCLVFEDSPFGVTAAKVAKMTAIAIPDEAMANSKYKHADQIIRKLADFDLAAYGLPPIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMZ2</accession>
    <name>A0A481QMZ2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Exodeoxyribonuclease I</fullName>
            <ecNumber evidence="2 12">3.1.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">sbcB</name>
        <name evidence="17" type="ORF">C3B55_00924</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 Mg(2+) ions per monomer.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.1" evidence="2 12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00924"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008852">
        <property type="term" value="F:exodeoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023607">
        <property type="entry name" value="Exodeoxyribonuclease_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034748">
        <property type="entry name" value="EXOI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034747">
        <property type="entry name" value="EXOI_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038649">
        <property type="entry name" value="EXOI_SH3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013620">
        <property type="entry name" value="Exonuc_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022894">
        <property type="entry name" value="Oligoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046">
        <property type="entry name" value="PTHR11046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08411">
        <property type="entry name" value="Exonuc_X-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000977">
        <property type="entry name" value="Exodeoxyribonuclease_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51785">
        <property type="entry name" value="EXOI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51784">
        <property type="entry name" value="EXOI_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 12 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ExoI SH3-like" evidence="15">
        <location>
            <begin position="194"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="ExoI C-terminal" evidence="16">
        <location>
            <begin position="350"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000977-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000977-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX82227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="479" mass="55593" checksum="8DCD3920696B5779" modified="2019-06-05" version="1">MTTIFWYDYEATGIHPRSDRPLQVAGIRTDLELNEVDKPVNLYCLPSDDILPHPAACMITGITPGMLAKNGLTEADFMTRVYAELAAPGTCSAGYNNLRFDDEMTRHSLYRNFFDPYTREWRCGNSRWDLINVVRTAYALRPEGIVWPQQDGRVTFKLERLTKANGIDHGQAHDALLDVRATIAMARLIRKKQPKLYEWLFQLRSKHRVMDQVHLLRPVVYISRYFSAERYYFGVVLPLAWHPHNRNALIVCDLGLDPQVLLDLDAKTLRKRLYTRRKDLAEGELPVPLKLLHINRCPVLAPLNVLWANDCRRIQFDIDACQALVLRLNDTQEVWLDKLTAIYAEEACVSSADPEQQLYNGFISNHDRRLCEQLRVAEPSQLASKQWSFDDYRLLELLFRYRARNFPDSLNSEERDRWKLFCQQRLTHVELGAPNTLLSFKRTCKELSVNATPLQCQVLDEWQMHADCLTTRLDLQSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN09</accession>
    <name>A0A481QN09_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RecBCD enzyme subunit RecB</fullName>
            <ecNumber evidence="8">3.1.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Exonuclease V subunit RecB</fullName>
            <shortName evidence="8">ExoV subunit RecB</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Helicase/nuclease RecBCD subunit RecB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">recB</name>
        <name evidence="12" type="ORF">C3B55_00987</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.</text>
            <dbReference type="EC" id="3.1.11.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding. Interacts with RecA.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The C-terminal domain has nuclease activity and interacts with RecD. It interacts with RecA, facilitating its loading onto ssDNA.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The N-terminal DNA-binding domain is a ssDNA-dependent ATPase and has ATP-dependent 3'-5' helicase function. This domain interacts with RecC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.5" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00987"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008854">
        <property type="term" value="F:exodeoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01485">
        <property type="entry name" value="RecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004586">
        <property type="entry name" value="RecB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF23">
        <property type="entry name" value="PTHR11070:SF23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00609">
        <property type="entry name" value="recB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8 9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="11">
        <location>
            <begin position="461"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="DNA-binding and helicase activity, interacts with RecC" evidence="8">
        <location>
            <begin position="1"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Nuclease activity, interacts with RecD and RecA" evidence="8">
        <location>
            <begin position="921"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="active site" description="For nuclease activity" evidence="8">
        <location>
            <position position="1114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="987"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="1101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="1114"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="1223" mass="138708" checksum="04A9096DCF5C63BA" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN18</accession>
    <name>A0A481QN18_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="8">High-affinity zinc uptake system membrane protein ZnuB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">znuB</name>
        <name evidence="8" type="ORF">C3B55_00998</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00998"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX82296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="268" mass="28109" checksum="44B5BC275619B860" modified="2019-06-05" version="1">MEIAASMVNVQLHAMLAGLALALVAGPLGSFMAWRRMAYFGDTLSHATLLGLAIGVLLDINPTISVIIGCLLLAMSLVTLQQRQSLPTDTLLGILAPSALSLGLVVLSLMHEVSIDLATYLFGDLLTISQMDLAWIIGGSATVLTILVAMWKPLLAISVHEELAIVEGLPVSTLRITIMLLIAVVIALAMKIVGVLLITSLLIIPAAATQRHASSPEQMAIGASLLGMLAVCGGLALSWFKNTPAGPSIVVTAAAMFLLSFIMPRQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN26</accession>
    <name>A0A481QN26_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dual-specificity RNA methyltransferase RlmN</fullName>
            <ecNumber evidence="14">2.1.1.192</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">23S rRNA (adenine(2503)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">23S rRNA m2A2503 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ribosomal RNA large subunit methyltransferase N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">tRNA (adenine(37)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">tRNA m2A37 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 16" type="primary">rlmN</name>
        <name evidence="16" type="ORF">C3B55_00956</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(2503) in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42916"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10282"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43332"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10485"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the radical SAM superfamily. RlmN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.192" evidence="14"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00956"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070040">
        <property type="term" value="F:rRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002935">
        <property type="term" value="F:tRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01849">
        <property type="entry name" value="RNA_methyltr_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040072">
        <property type="entry name" value="Methyltransferase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027492">
        <property type="entry name" value="RNA_MTrfase_RlmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004383">
        <property type="entry name" value="rRNA_lsu_MTrfase_RlmN/Cfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544">
        <property type="entry name" value="PTHR30544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006004">
        <property type="entry name" value="CHP00048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00275">
        <property type="entry name" value="adenosine_C2_methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00048">
        <property type="entry name" value="rRNA_mod_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14 16" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 14 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="15">
        <location>
            <begin position="102"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="14">
        <location>
            <begin position="170"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="14">
        <location>
            <begin position="224"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" description="S-methylcysteine intermediate" evidence="14">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="QAX82257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="382" mass="43113" checksum="FBFDC0FED08A0FB9" modified="2019-06-05" version="1">MTISTVKSNLLGMTKQKMEHFFDLIGEKRFRAAQVMKWIHYFGIDDFDAMTNVSKTLRNKLKIIAEVRGPEVVSEDISSDGTRKWLVRVASGSCVETVYIPQGKRGTLCISSQAGCALDCSFCFTGKQGFNSNLTVDEIIGQIWLANKSFSGVQTGVNRVVTNVVMMGMGEPLLNFDNVVAAMHLMIDDFGYCIPKRRLTLSTSGVVPMLNKLSEHINISLTLSLHAPNDKLRNQLVPINKKYPLKILLESCQRYMATLGNKRMLTIAYTMLKDINDKIEHAVEMIELLKNIPCKINLIPFNPFPYSSYERPSNNAIRRFQNRLRQAGYNVTIRTTRGEDIDAACGQLVGQIIDRTSRSENYITMHKVNVTKDMQQITLNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN27</accession>
    <name>A0A481QN27_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetylornithine aminotransferase</fullName>
            <shortName evidence="2">ACOAT</shortName>
            <ecNumber evidence="2">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">aruC</name>
        <name evidence="2" type="synonym">argD</name>
        <name evidence="4" type="ORF">C3B55_00894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + N(2)-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="2">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00894"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017652">
        <property type="entry name" value="Ac/SucOrn_transaminase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03246">
        <property type="entry name" value="arg_catab_astC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="226"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="412" mass="44347" checksum="70A69123483D715D" modified="2019-06-05" version="1">MFVEQAPVQRADFDQVMVPNYAPAAFIPVRAVGSRVWDQTGRELIDFAGGIAVNVLGHAHPVLVVALTEQANKLWHVSNVFTNEPALRLAHKLIDATFAERVFFCNSGAEANEAAFKLARRVALDCFGAKKYEIISALNSFHGRTLFTVNVGGQSKYSNGFGPKITGIIHVPYNDLDALKSVVSDHTCAIVLEPIQGEGGVLPAELTYLQGARELCDVNNALLVFDEVQTGMGRSGHLFAYQHYDVTPDILTSAKGLGGGFPIAAMLTREDLAKHLIVGTHGTTYGGNPLACAVAEAVIDAINVPEVLAGVNAKHDLFKVRLEQIGKKYGIFTKVRGIGLLLGCVLSDSFTGKAKDVFNAAEKENLMILQAGPNVVRFAPSLVIEDTDIQEGLDRFECAVKTLTQTSYHIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN44</accession>
    <name>A0A481QN44_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpmH</name>
        <name evidence="6" type="ORF">C3B55_01018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01018"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00391">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000271">
        <property type="entry name" value="Ribosomal_L34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020939">
        <property type="entry name" value="Ribosomal_L34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14503">
        <property type="entry name" value="PTHR14503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00468">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01030">
        <property type="entry name" value="rpmH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00784">
        <property type="entry name" value="RIBOSOMAL_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="44" mass="5136" checksum="A86F7F831641EAB7" modified="2019-06-05" version="1">MKRTFQPSNIKRARTHGFRARMATKNGRAILSRRRAKGRIRLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN68</accession>
    <name>A0A481QN68_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00922"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="43" mass="4684" checksum="1DB45783CB9B7DC3" modified="2019-06-05" version="1">MQPKISCSLSVVVVADVSTCWNIKLHSIQVAIGIREALDVFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN91</accession>
    <name>A0A481QN91_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Thiol:disulfide interchange protein DsbD</fullName>
            <ecNumber evidence="19">1.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein-disulfide reductase</fullName>
            <shortName evidence="19">Disulfide reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 21" type="primary">dsbD</name>
        <name evidence="21" type="ORF">C3B55_00828</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>[protein]-dithiol + NAD(+) = [protein]-disulfide + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:18749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>[protein]-dithiol + NADP(+) = [protein]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:18753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 19">Cell inner membrane</location>
            <topology evidence="4 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the thioredoxin family. DsbD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.8" evidence="19"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00828"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047134">
        <property type="term" value="F:protein-disulfide reductase (NAD(P)) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02953">
        <property type="entry name" value="DsbDgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00399">
        <property type="entry name" value="DbsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035671">
        <property type="entry name" value="DsbD_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028250">
        <property type="entry name" value="DsbDN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036929">
        <property type="entry name" value="DsbDN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022910">
        <property type="entry name" value="Thiol_diS_interchange_DbsD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32234:SF0">
        <property type="entry name" value="PTHR32234:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11412">
        <property type="entry name" value="DsbC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74863">
        <property type="entry name" value="SSF74863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 19" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 19" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="17 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12 19" id="KW-0249">Electron transport</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="15 19" id="KW-0520">NAD</keyword>
    <keyword evidence="14 19 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18 19" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-0732">Signal</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 19" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019874692" description="Thiol:disulfide interchange protein DsbD" evidence="19">
        <location>
            <begin position="19"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="277"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="20">
        <location>
            <begin position="444"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="19">
        <location>
            <begin position="117"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="19">
        <location>
            <begin position="488"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="QAX82139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="567" mass="61521" checksum="3BC3714DC59B3C90" modified="2019-06-05" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN92</accession>
    <name>A0A481QN92_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative efflux pump periplasmic linker TtgA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ttgA</name>
        <name evidence="11" type="ORF">C3B55_00734</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00734"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043602">
        <property type="entry name" value="CusB_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00529">
        <property type="entry name" value="CusB_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="HlyD_D23" evidence="10">
        <location>
            <begin position="61"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="CusB_dom_1" evidence="9">
        <location>
            <begin position="278"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX82054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="372" mass="40604" checksum="E94381B37DA5754F" modified="2019-06-05" version="1">MQLKLAIITLVTTIAFTSLLSGCKKEEEVLPTQTSEVGVVTIQPQTFILTSNLPGRTTAYRIAEVRPQVTGIILKRFFQEGSNVKEGQQLYQITSSIYDASLRVAQANLAQTRAIANRYKQLVNEQAVSRQEYDTAVSNQMISEANVQVALINVRYTKVLAPISGRIGRSSVTEGALVSNGQANAMAIIQQLDPIYVDVMQSSAEILKLRHHLESGQLKQASENNTKVKLTLEDGIAYGLDGQLEFSEVSVDQTTGSVTLRATFPNPDHTLLPGMFVHAQLQTGINNQAILVPQQGVTRNFKGTPTALIVNKDNKVEQRQLIANSTDGAYWLVEKGLDAGDRVITEGLQFIKPGIEVKVKNADNTKPASKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN99</accession>
    <name>A0A481QN99_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Outer membrane protein assembly factor BamB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">bamB</name>
        <name evidence="5" type="ORF">C3B55_00952</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BamB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00952"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00923">
        <property type="entry name" value="OM_assembly_BamB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017687">
        <property type="entry name" value="BamB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03300">
        <property type="entry name" value="assembly_YfgL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019874217" description="Outer membrane protein assembly factor BamB" evidence="4">
        <location>
            <begin position="20"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="390" mass="41701" checksum="02484FF9D709207C" modified="2019-06-05" version="1" precursor="true">MSCWKYAVLIFLAMLTVLAAGCSSNSAIKLPLAVPIRFTETVVLQKQWSRYVGDGQGALYNLLQPAIDGTNIVVTDATGVIMAMDYMNGDVKWKKDLELPVSGGVGAGYGMVLISTLKGDVVALDSTTGEEKWRTCVTREVLAAPATNGDVIVVQTQDDCVIVLDAATGKHRWSYDSTPTVLTLRGTSSPIVIDNLAVVGLSTGKVVALDIQNNIPVWETRVAIPQGRSELERIVDIDGALLLVGETIYVATYQGRVAALDLQSGRINWHRDASSYAGIAQEFGKIYVSLASGIVESVDEHSNATLWSSDSLAYCQLSAPAVFSSYVAVGDAEGYLNLLSRLDGRLIGRECIDSDGLRVRPLVIGNILYVFGNSGKLEAMTIKSITDASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNB0</accession>
    <name>A0A481QNB0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Pyruvate kinase</fullName>
            <ecNumber evidence="4 12">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pykA</name>
        <name evidence="15" type="ORF">C3B55_00925</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 12"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00925"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PTHR11817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 12 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PK" evidence="13">
        <location>
            <begin position="4"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="PK_C" evidence="14">
        <location>
            <begin position="362"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="QAX82228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="483" mass="51805" checksum="D2A3DF58477CDF83" modified="2019-06-05" version="1">MSVRRTKIIATLGPASNSPDILEQLILAGLNVARLNLSHGTPDEHKASAKLVRSLAAKHGRFVALLGDLQGPKIRLAKFINKRIELKIGDQFTFSTNHPLTKGNQTIVGVDYPDLVKDCGVGDKLLLDDGRVVMRVDTATKTELNCTVIIGGTLSDHKGINRRGGGLTAPALTEKDKSDIKLATEIEVDYLAVSFPRDAADMEYARKLSTEAGGAAWLVAKIERAEAVADDATLDGLIKASDAIMVARGDLGVEIGDAELIGIQKKIILHARRYNKAVIVATQMMESMLQNPIPTRAEVSDVANAVLDYTDAVMLSAESAAGPYPLEAIQAMARICVGAEKHPTSKTSSHRIGTMFESCDQSIALAAMYTANHFPGVKAIIALTESGYTPLIMSRIRSSVSIYAFTPHLAAQARTALFRGVYTIPFDPASLAPNEVSQKAIDELVKRGVVETGDWIILTKGDSYHTTGGTNGMKILHVGDLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNC8</accession>
    <name>A0A481QNC8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cysteine desulfurase IscS</fullName>
            <ecNumber evidence="9">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">iscS</name>
        <name evidence="12" type="ORF">C3B55_00964</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00964"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="PTHR11601:SF34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02006">
        <property type="entry name" value="IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="11">
        <location>
            <begin position="5"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="9">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="9">
        <location>
            <begin position="203"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="9">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="9">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <note>ligand shared with IscU</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="QAX82265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="404" mass="44590" checksum="DCEDB33C4068BB7F" modified="2019-06-05" version="1">MKLPIYLDYSATTPVDPRVAQKMSECLLTDGNFGNPASRSHVFGWKAEEAVENARRQVADLVGADPREIVWTSGATESDNLAIKGAAHFYATKGRHLITTKIEHKAVLDTMRQLEREGFEVTYLKPTTDGIVTPAMIEAVLRKDTILVSVIHVNNEIGAINDIASIGELTRLKGILLHVDAAQSTGKVDIDLSKLKVDLMSFSAHKTYGPKGIGALYVSRKPRVRIEAIMHGGGHERGMRSGTLATHQIVGMGEAFHLAKEDMSAENMRIKVLSDRFYNQVKSLEELYINGSMTTRVPHNLNLSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRAIDRNDELAHSSIRFTFGRFTTEEQVDFAAQKVCDAVNKLRALSPLWDMYKDGIDLSKIKWAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNI3</accession>
    <name>A0A481QNI3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="7">TC-AMP synthase</shortName>
            <ecNumber evidence="7">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">tRNA threonylcarbamoyladenosine biosynthesis protein TsaC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">tsaC</name>
        <name evidence="9" type="ORF">C3B55_01007</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + hydrogencarbonate + L-threonine = diphosphate + H2O + L-threonylcarbamoyladenylate</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SUA5 family. TsaC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01007"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01852">
        <property type="entry name" value="TsaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023535">
        <property type="entry name" value="TC-AMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF8">
        <property type="entry name" value="PTHR17490:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="8">
        <location>
            <begin position="4"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="185" mass="20618" checksum="7A66DD9B44B2781F" modified="2019-06-05" version="1">MASKWSVLEAKREIRAGAVIAYPTEAVWGLGCDPWNKETVNRLLAIKNRSVDKGLILVADNICQFNFLLDGFPNIWVERMVNTWPGPNTWLVPHQNLLPEWVTGMHDTVALRVSHHPLVRDLCALVGPIISTSANPHGRPAARTRIRVEQYFRGQVDMVLGGSLGGLENPSLIRHLATGEVVRYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNM8</accession>
    <name>A0A481QNM8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Threonine synthase</fullName>
            <ecNumber evidence="7">4.2.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">thrC</name>
        <name evidence="7" type="ORF">C3B55_00937</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="7"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00937"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thr_synth_N" evidence="6">
        <location>
            <begin position="2"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="PALP" evidence="5">
        <location>
            <begin position="94"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX82239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="469" mass="52347" checksum="1D5FDCEF3376457E" modified="2019-06-05" version="1">MRYISTRGHAPSLSLEGALLAGLASDGGLYVPENLPRFTHKEIASWVGLPYNELAFRVMRQFVTGSIPDADFKKIIEETYGVFSHKAVAPLCQLNSNEWVLELFHGPTLAFKDFSLQLLGRLLGYVLERCAKLVVILGATSGDTGSAAIEGCKHCENVDIFILHPHQRVSEIQRRQMTTVFSKKIHNIAIKGNFDDCQEMVKSSFADQSFLSGKRLIAVNSINWVRIMAQIVYYFYASLQLGGPARSVSFSVPTGNFGNIFAGYLARNMGLPINQLIVATNRNDILHRFISSNRYIKDTMHVTLSPSMDIMVASNFERLLFDIYSRNSVVLAKLVISLKQGGGFSIEQGRWIEIRKLFDSLAVDDIQTCKTIAEVYAQTGKLLDPHTAIGVKAARDCRRSLNTPMVILGTAHPIKFPEAVEKAGFNKALELPEHLADLFEREEHYTVLPNNLQVMQTFISQNFNSSQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNP5</accession>
    <name>A0A481QNP5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lipid A biosynthesis lauroyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.241</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Kdo(2)-lipid IV(A) lauroyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">htrB_1</name>
        <name evidence="8" type="synonym">lpxL</name>
        <name evidence="9" type="ORF">C3B55_00823</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of laurate from lauroyl-acyl carrier protein (ACP) to Kdo(2)-lipid IV(A) to form Kdo(2)-(lauroyl)-lipid IV(A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-Kdo-(2-&gt;4)-alpha-Kdo-(2-&gt;6)-lipid IVA + dodecanoyl-[ACP] = dodecanoyl-(Kdo)2-lipid IVA + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:28442"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:60365"/>
            <dbReference type="ChEBI" id="CHEBI:61524"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="EC" id="2.3.1.241"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LpxL/LpxM/LpxP family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.241" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00823"/>
    <dbReference type="UniPathway" id="UPA00030"/>
    <dbReference type="UniPathway" id="UPA00360">
        <property type="reaction ID" value="UER00485"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008913">
        <property type="term" value="F:lauroyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036104">
        <property type="term" value="P:Kdo2-lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07984">
        <property type="entry name" value="LPLAT_LABLAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01942">
        <property type="entry name" value="Lipid_A_LpxL_LpxP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004960">
        <property type="entry name" value="LipA_acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011920">
        <property type="entry name" value="Lipid_A_LpxL_LpxP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30606">
        <property type="entry name" value="PTHR30606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03279">
        <property type="entry name" value="Lip_A_acyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026649">
        <property type="entry name" value="MsbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02207">
        <property type="entry name" value="lipid_A_htrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXXXXD motif" evidence="8">
        <location>
            <begin position="131"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="313" mass="36588" checksum="D071AE658909BCE9" modified="2019-06-05" version="1">MDSPHFRVVFLHWRFWLLWLGLGMLWLINQLPYRVLLTIGRLLGVGMYRMASNRHCIARRNLELCFPEKSSGERKRLLKENFASTGIAFFEMAMSWWWPKSRLERLAHVEGLEHLKQAHLNGKGVILMALHFTTLEIGAALLGQKYTIDGMYREHSNLLFDFIQRRGRERHNLNSIAVERNDVRGMIKLLRTGRFIWYAPDQDYGAKQSIFVPLFGIKAATVKSTSTFARLGKALVMPFTQKRLTNGNGYCLVIHPPLTNFPSESDEIDCMRINQWIEATVRECPEQYLWAHRRFKSRPPGEVKLYEKCRHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QPI4</accession>
    <name>A0A481QPI4_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxygen-dependent coproporphyrinogen-III oxidase</fullName>
            <shortName evidence="5">CPO</shortName>
            <shortName evidence="5">Coprogen oxidase</shortName>
            <shortName evidence="5">Coproporphyrinogenase</shortName>
            <ecNumber evidence="5">1.3.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">hemF</name>
        <name evidence="6" type="ORF">C3B55_01006</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IX</text>
            <dbReference type="Rhea" id="RHEA:18257"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="1.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the aerobic coproporphyrinogen-III oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.3" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01006"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00322"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00333">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001260">
        <property type="entry name" value="Coprogen_oxidase_aer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036406">
        <property type="entry name" value="Coprogen_oxidase_aer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018375">
        <property type="entry name" value="Coprogen_oxidase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755">
        <property type="entry name" value="PTHR10755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01218">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000166">
        <property type="entry name" value="Coproporphyri_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00073">
        <property type="entry name" value="COPRGNOXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102886">
        <property type="entry name" value="SSF102886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01021">
        <property type="entry name" value="COPROGEN_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for dimerization" evidence="5">
        <location>
            <begin position="241"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="259"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for dimerization" evidence="5">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="310" mass="35814" checksum="A909DB21F680A051" modified="2019-06-05" version="1">MITRTEAVKAYLLDLQDRICNAIEAFEEDTRFIEDTWIQPAGSNGHTRVIENGLVIEKGGVNFSHVFGNKLPHSASAHRHELAGRDFESLGVSLVMHPHNPYIPTSHANVRFFITKKESEEPVWWFGGGFDLTPYYGNEEDCIHWHFVAQQACKPFGPDVYSRYKAWCDNYFYIKHRNEPRGIGGLFFDDLNEWDFDTCFAFIRTIGDAYIDAYLPIVQRRKAMAYTEQQREFQKFRRGRYVEFNLIYDRGTLFGLQAGGRTESILISLPSQVRWGYAWKAEAGSEEARLTDYFLQNHDWLGVHASKASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QPK1</accession>
    <name>A0A481QPK1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Beta sliding clamp</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">dnaN</name>
        <name evidence="14" type="ORF">C3B55_01016</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a ring-shaped head-to-tail homodimer around DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the beta sliding clamp family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_01016"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00140">
        <property type="entry name" value="beta_clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001001">
        <property type="entry name" value="DNA_polIII_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022635">
        <property type="entry name" value="DNA_polIII_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022637">
        <property type="entry name" value="DNA_polIII_beta_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022634">
        <property type="entry name" value="DNA_polIII_beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30478">
        <property type="entry name" value="PTHR30478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00712">
        <property type="entry name" value="DNA_pol3_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02767">
        <property type="entry name" value="DNA_pol3_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02768">
        <property type="entry name" value="DNA_pol3_beta_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000804">
        <property type="entry name" value="DNA_pol_III_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00480">
        <property type="entry name" value="POL3Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00663">
        <property type="entry name" value="dnan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6 10 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_beta" evidence="11">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_2" evidence="12">
        <location>
            <begin position="130"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_3" evidence="13">
        <location>
            <begin position="248"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX82314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="367" mass="41021" checksum="59D01EA0A39C665D" modified="2019-06-05" version="1">MHFTIQREVLLKPLQLVAGIVERRQALQVISNVLLVIKDQQLYLTGTDLEVELVGRVQLTSPAESGEITVPARKLMDICKSLHNDTLINIKLDQQKLLVRAGRSRFTLSTLPANNFPTVEDSPGSLYCSIMQSKFRCLIERTSFAMAQQDVRYYLNGMLLEVSDGIIRAVATDGHRLAMCSMKVDVGQPDCYQAIVPRKGILELLRLLTDPDSNINIALNQHHIRAIAGEFTFTSKLINGKFPDYKCVLPKNGDKLVIGNRQALREAFSRTAILSNEKHRGILLQISNSQLKIEAKNPEQEEAEEEVGVEYNGDSLEIGFNVSYLLDVLSVITTKQVCMVFSDSNSSALIQEPNNNDSSYVVMPMRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QK55</accession>
    <name>A0A481QK55_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.107</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hemX</name>
        <name evidence="3" type="ORF">C3B55_00041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.107" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00041"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007470">
        <property type="entry name" value="HemX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38043">
        <property type="entry name" value="PTHR38043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04375">
        <property type="entry name" value="HemX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="366" mass="41152" checksum="309A72E44C06D601" modified="2019-06-05" version="1">MSDTTIHKNEAQPLLQAPVELQLAKLHHSNILAVIGMFLGVAGAAVGIWSILQAHMLKASNQQHLNQVQTLDDQFQSLKQSQQQLTARIVQLPTTDEFENCRYLITKLQGDQQRLSDHLETVLGLSHQDWYLAEAEHLIRLASLRLSALQDINSAYLLVQSADEILRKQSDPGAYIVHEQLAKSLAALRNIDQPDRNGLYLQLIALREQAMQLASIAPEYQLTEQVPEEFQTSDTRHYLSHWWKQIFRYFRIDFKPDDNVHPLRAGQSLNQAHIALNLTLEQAQWAVLNNDKIVYSRSMGEARKMLQNNFNQDNQQSKAILAQIAELEPKTVSVVTPSLAASLAAVQAYLSVYAPKASAGVPATQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKH3</accession>
    <name>A0A481QKH3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">trxA</name>
        <name evidence="5" type="ORF">C3B55_00049</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00049"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="3">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="109" mass="12020" checksum="255EDCE8FE7032F7" modified="2019-06-05" version="1">MSNNLIKHVTDATFETEVLKAQGLVLVDYWAEWCSPCKMIAIVLDDIAIAYEGKLIVAKLNIDDNQETPAKQGVRSIPTLMLFKHGNATATKVGALSKSQLQTFLDENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKM6</accession>
    <name>A0A481QKM6_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="1">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">C3B55_00087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00087"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PTHR10146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00044">
        <property type="entry name" value="TIGR00044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01211">
        <property type="entry name" value="UPF0001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_N" evidence="4">
        <location>
            <begin position="10"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1 2">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="225" mass="24494" checksum="A6FC92A2202C6DA1" modified="2019-06-05" version="1">MSTIANNISQISKRIRAAAQAVQRDACSIHLLAVSKTKPSQAVREAYAAGMQDFGENYLQEALSKQAELTDLPLNWHFIGPIQSNKTRAIAKNFAWVHSVDRLKIAQRLSEQRPADLPPLNICIQANISGETSKSGCTPDQLVLASAISALPRLKLRGLMVIPKQTKDRAAQDAAFTTLRDLQSSLKLPLDTLSMGMSHDLESAISKGATWIRVGTALFGARDYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QKT4</accession>
    <name>A0A481QKT4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Inner membrane ABC transporter permease protein YdcV</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ydcV</name>
        <name evidence="6" type="ORF">C3B55_00062</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00062"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="60"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="294" mass="32275" checksum="63F033ACBA870746" modified="2019-06-05" version="1">MRRFSFSGFMLGAGLLFVYLPMLILVIYSFNGSKLVTVWSGWSIKWYASLLDNTQLIVSIGRSLEIACYTAVAAVALGTLAAFVLTRITNFKGRTLFCGLVTAPLVMPEVITGLSLLLLFVAMAQIIGWPQERGIVTIWIAHTTFCAAYVVVLVSARLRELDLSIEEAAMDLGARPWKVFFLITIPMIAPSLVAGGMMSFVLSLDDLVLASFVSGPGSTTLPMAVFSAMRLGVKPEINAVASLILLIVSLVTFIVWLFIRRAEEMRKKAVQQTIDEAATDNWLQSDKHSTSMFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL06</accession>
    <name>A0A481QL06_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00149"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="41" mass="4747" checksum="FEF843D0E41BD508" modified="2019-06-05" version="1">MDLNSKVDKITSGMVMIKKLIVYPEACYTYFNINNKGVKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL08</accession>
    <name>A0A481QL08_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00125"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="51" mass="5568" checksum="E86D9FD4DDB65AF9" modified="2019-06-05" version="1">MVVNFSMFSYRSISKCVPIATMSHCVKLPSHLAATVQLNFVVPSNANLVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL12</accession>
    <name>A0A481QL12_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase</fullName>
            <ecNumber evidence="2">2.5.1.145</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">lgt</name>
        <name evidence="3" type="ORF">C3B55_00070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-cysteinyl-[prolipoprotein] = H(+) + S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[prolipoprotein] + sn-glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:56712"/>
            <dbReference type="Rhea" id="RHEA-COMP:14679"/>
            <dbReference type="Rhea" id="RHEA-COMP:14680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="ChEBI" id="CHEBI:140658"/>
            <dbReference type="EC" id="2.5.1.145"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; lipoprotein biosynthesis (diacylglyceryl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Lgt family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.145" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00070"/>
    <dbReference type="UniPathway" id="UPA00664"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008961">
        <property type="term" value="F:phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01147">
        <property type="entry name" value="Lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001640">
        <property type="entry name" value="Lgt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30589">
        <property type="entry name" value="PTHR30589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01790">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00544">
        <property type="entry name" value="lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01311">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)</name>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="265" mass="30260" checksum="9B102A8F6EDBABD7" modified="2019-06-05" version="1">MLFYPKINPVVVAIGPLQIHWYGLMYLIGIGSVWLLASKRLNRLKPTWTKEEFSDIVFWMSIGMIVGGRLGYVLFYALPVYIANPMLIFAVWKGGMAFHGGFVGVIISAWLFVCRNRKLFFPLMDLIAPFVPIGLGAGRIGNFVNAELWGKPSDVPWAMVFPLFSDPAQLPRHPSQLYQFGLEGVALFLILYIFSRKPRPTMAISGMFLLLYGIFRCIVELIRMPDAQLGYLAWGWLTMGQILSMPMILFGLSLLWWAYNFRQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL37</accession>
    <name>A0A481QL37_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Primosomal protein N'</fullName>
            <ecNumber evidence="9">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-dependent helicase PriA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">priA</name>
        <name evidence="11" type="ORF">C3B55_00182</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the primosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the helicase family. PriA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="9"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00182"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00983">
        <property type="entry name" value="PriA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005259">
        <property type="entry name" value="PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041222">
        <property type="entry name" value="PriA_3primeBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042115">
        <property type="entry name" value="PriA_3primeBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041236">
        <property type="entry name" value="PriA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040498">
        <property type="entry name" value="PriA_CRR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17764">
        <property type="entry name" value="PriA_3primeBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18074">
        <property type="entry name" value="PriA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18319">
        <property type="entry name" value="PriA_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00595">
        <property type="entry name" value="priA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="215"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="440"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="467"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QAX81547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="739" mass="82722" checksum="0DD3C2EEFD97E74F" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL43</accession>
    <name>A0A481QL43_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LPS-assembly protein LptD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 5" type="primary">lptD</name>
        <name evidence="5" type="ORF">C3B55_00120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Together with LptE, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the lipopolysaccharide transport and assembly complex. Interacts with LptE and LptA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LptD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00120"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015920">
        <property type="term" value="P:lipopolysaccharide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010033">
        <property type="term" value="P:response to organic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01411">
        <property type="entry name" value="LPS_assembly_LptD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020889">
        <property type="entry name" value="LipoPS_assembly_LptD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007543">
        <property type="entry name" value="LptD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005653">
        <property type="entry name" value="OstA-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04453">
        <property type="entry name" value="LptD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03968">
        <property type="entry name" value="LptD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019875052" description="LPS-assembly protein LptD" evidence="2">
        <location>
            <begin position="28"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="domain" description="OstA-like_N" evidence="3">
        <location>
            <begin position="121"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="LptD" evidence="4">
        <location>
            <begin position="365"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="908" mass="104328" checksum="906EA21291C509BB" modified="2019-06-05" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QL65</accession>
    <name>A0A481QL65_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE</fullName>
            <ecNumber evidence="6">2.1.1.163</ecNumber>
            <ecNumber evidence="6">2.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">2-methoxy-6-polyprenyl-1,4-benzoquinol methylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Demethylmenaquinone methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">ubiE</name>
        <name evidence="7" type="ORF">C3B55_00179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9539"/>
            <dbReference type="Rhea" id="RHEA-COMP:9563"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18151"/>
            <dbReference type="ChEBI" id="CHEBI:55437"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.163"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:28286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="EC" id="2.1.1.201"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Quinol/quinone metabolism; menaquinone biosynthesis; menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.163" evidence="6"/>
    <dbReference type="EC" id="2.1.1.201" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00179"/>
    <dbReference type="UniPathway" id="UPA00079">
        <property type="reaction ID" value="UER00169"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043333">
        <property type="term" value="F:2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043770">
        <property type="term" value="F:demethylmenaquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102094">
        <property type="term" value="F:S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102955">
        <property type="term" value="F:S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102027">
        <property type="term" value="F:S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="4 6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="138"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="266" mass="29504" checksum="1FDA19EFA6AF6A78" modified="2019-06-05" version="1">MHYLLVIAIMDDQYKSSSCVEPATHFGFKSVPESQKAANVAKVFNSVAAKYDLMNDVLSGGMHRLWKRFTIELSGVRTGNRVLDIAGGTGDLAAKFSRLVGPTGQVALVDINSSMLRIGRDRLLDKGVSGNIKFIQADAEKLPFLDNYFDCVTIAFGLRNVTHKEDAIRSMLRVLKPGGRLLVLDFSKPTNALIAKVYNTYSFTLIPLLGKMITNDADSYRYLTESIRMHPDQETLKLMIVKAGFYRASYHNITSGIVALHRGIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLD3</accession>
    <name>A0A481QLD3_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00354"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX81708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="51" mass="5749" checksum="595AEE655F851219" modified="2019-06-05" version="1">MLNLEIKQITYAYTATIGLRSPEAPLQLNNRSHWRGGHVLLQSPLCNCIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLD9</accession>
    <name>A0A481QLD9_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Riboflavin synthase</fullName>
            <ecNumber evidence="4">2.5.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ribE</name>
        <name evidence="4" type="ORF">C3B55_00312</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00312"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="PTHR21098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="98"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="98"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX81667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="208" mass="22542" checksum="1E2920E1CF4B5D67" modified="2019-06-05" version="1">MFTGIIESIGSIRLITPKDGDIRLLVEAGKLYLGDVKLGDSIAVSGACLTVIDLLGNSFVADVSQETLDRTVISDFKIGSLVNLEKSIMPTTRLGGHLVSGHVDGVGEVVARSENARAVKFRIRAPNVLAKYIANKGSITVDGISLTVNAVNGPEFELTIIPYTLSKTIMAFYWAGCRVNLEVDLFARYLERLLLGEKAAEPYNGEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLG2</accession>
    <name>A0A481QLG2_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-octaprenylphenol hydroxylase</fullName>
            <ecNumber evidence="5">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ubiI</name>
        <name evidence="5" type="ORF">C3B55_00054</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00054"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="4">
        <location>
            <begin position="4"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX81429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="407" mass="45116" checksum="F6D5A3B55710124D" modified="2019-06-05" version="1">MDMYTDVLIIGAGMVGSTMALALQSSSLKVLLLDSSPLIVKPFNQNATFESRVSALSATSQRILEQLGVWDGIVSRRVSSYSKIEVWDGSGTGYVDFSVASVHAEVLGYIVENRVIQDAVLDRLHDCDLNLLANAHLEEMSRSGNDWLLTLADGCTLRASLVIAADGANSTVRRLTGAATRAWDYLHNAIVTSVRTSQSHQHTVWQRFTDTGPLAFLPLVHNKQENWCSIVWSTTPVETERLMLLNDDSFCYELERAFEGRLGAVLSTEERVCVPLRQSHAKRYVCEGLALIGDAAHVIHPLAGQGVNLSFLDVVVLAEVLLMATKRGEYLADAKVLSRYERRRMLHNITLMLAMEGFERLFQADQLSLRWLRNVGLKLINQMPESKAIFMRKALGLTGDLPSLVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLL6</accession>
    <name>A0A481QLL6_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stringent starvation protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">sspB</name>
        <name evidence="2" type="ORF">C3B55_00537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00537"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007481">
        <property type="entry name" value="SspB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036760">
        <property type="entry name" value="SspB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37486">
        <property type="entry name" value="PTHR37486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04386">
        <property type="entry name" value="SspB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005276">
        <property type="entry name" value="SspB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101738">
        <property type="entry name" value="SSF101738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="146" mass="16553" checksum="B45D2280A6079489" modified="2019-06-05" version="1">MNSIRPYLVRALYEWIIENHCTPYMLVNSTFPKVDVPQSSAIDGQIVLNISTNAVRYLHIGNEAVSFEGCFSGVPHKLFVPIRAILGIYIRENSYGMLFDLELPFEDNESIRSKDSDNLSIPKSKLSHQNDGPSLKVIRPNFKIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLL8</accession>
    <name>A0A481QLL8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UPF0114 protein C3B55_00350</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">C3B55_00350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UPF0114 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00350"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00143">
        <property type="entry name" value="UPF0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005134">
        <property type="entry name" value="UPF0114"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020761">
        <property type="entry name" value="UPF0114_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38596">
        <property type="entry name" value="PTHR38596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03350">
        <property type="entry name" value="UPF0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00645">
        <property type="entry name" value="HI0507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="162" mass="18365" checksum="6B6F5242ECE47F47" modified="2019-06-05" version="1">MERFIENAMYASRWLLAPIYFGLSLGLLALALKFFQEAIHLLPSVFSMTESELILVLLSLIDMALVGGLLVMVMISGYENFVSQLDIYEDKERLNWLGTMDSSSLKMKVAASIVAISSIHLLRIFMDAKNVDPQHLMWYVIIHITFVISAFAMGYLDKVTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-06-02" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLM5</accession>
    <name>A0A481QLM5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glutarate-semialdehyde dehydrogenase DavD</fullName>
            <ecNumber evidence="6">1.2.1.20</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">davD</name>
        <name evidence="6" type="ORF">C3B55_00141</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.20" evidence="6"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00141"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047949">
        <property type="term" value="F:glutarate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009013">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010102">
        <property type="entry name" value="Succ_semiAld_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01780">
        <property type="entry name" value="SSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="19"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX81509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="482" mass="52573" checksum="DCD889A762D565DE" modified="2019-06-05" version="1">MHLKDTKLFRQQAFIDGAWVDADNGQTIKVNNPATGEILGIVPKMGVAETRRAIEAANRALPTWRILTAKDRANKLRCWFELIIENQDDLALLITLEQGKPLAEAKSEVIYAASFIEWFAEEAKRIYGDIIPSHQLDKRFFVIKQPIGVTAAITPWNFPAAMITRKAGPALAAGCTMVLKPALQTPFSAFAMAELAQRAGIPKGVFSVIAGSAEDIGSELTSNPIVRKLSFTGSIEIGRQLMAKCTKDIKSISLELGGNAPFIVFDDANLDNAVKGAITSRYRNNGQTCIAANRLYIQNSVYDAFAEKLKVAVSKLKIGNGLEDSTTIGPLIDKNAIAKVQEHIMDAVSKGAKLLAGGKVIKDNFFEPTILVDVPKDAIFAKEETFGPVAPLFRFKDEAEVISMSNDTEFGLASYLYTCNLDRIFRVAEALEYGMVGINTGLISNETVPFGGIKASGLGREGSKYGIEEYLEIKYLCLNVFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLN0</accession>
    <name>A0A481QLN0_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Signal peptidase I</fullName>
            <ecNumber evidence="5">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">lepB</name>
        <name evidence="8" type="ORF">C3B55_00558</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00558"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019757">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Lys-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00760">
        <property type="entry name" value="SPASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S26" evidence="7">
        <location>
            <begin position="60"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="284" mass="31888" checksum="C75E2D2FCCF164DE" modified="2019-06-05" version="1">MSLNFPLLLVIAAAVCGLLALLDLLFFTPSRRAAIASYEGSVSQPDAVVIKRLNKKPLLVEYGKSFFPVLCIVLVLRSFLVEPFQIPSGSMIPTLDVGDFILVNKFSYGIRLPVINKKIISVGDPQRGDVMVFRYPSNQNINYIKRVVGLPGDVIRYTSNKRLFINGELVTEKLISTEPSTLGSAELYQEKLGAVEHEIRKEMSIYYLLSDTEWTVPAGHYFMTGDNRDNSNDSRYWDDPNIPKNLLGMVPDENIVGKAFVIWMSWSEPKLSHLPNFSRAGLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLP7</accession>
    <name>A0A481QLP7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13 14">UDP-N-acetylmuramoylalanine--D-glutamate ligase</fullName>
            <ecNumber evidence="5 13 14">6.3.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">D-glutamic acid-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 17" type="primary">murD</name>
        <name evidence="17" type="ORF">C3B55_00578</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13 14">
            <text>ATP + D-glutamate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13 14">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.9" evidence="5 13 14"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00578"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00639">
        <property type="entry name" value="MurD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005762">
        <property type="entry name" value="MurD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01087">
        <property type="entry name" value="murD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 13 14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8 13 14" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 13 14" id="KW-0133">Cell shape</keyword>
    <keyword evidence="12 13 14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 13 14" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="16">
        <location>
            <begin position="112"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="15">
        <location>
            <begin position="305"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="QAX81911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="446" mass="48129" checksum="37C5904B9150A123" modified="2019-06-05" version="1">MITADHFYIVVGLGNSGMSIVRFLVNRGMLFAVADTRNNPPGLAALRRTHPHLEVCCGELDVEFLCRADELYVSPGVDLATYALQAAAARGVKLSGDIDLFARYAKAPIVAISGSNAKSTVTTLVGEMAAMAGKRVAVGGNIGMLALDLLSDDIELYVIELSSFQLDTTHNLSAEVATVLNISEDHMNRYSSFTAYCLAKHRIFQSVKQLVVNRHDALSCPLIGENQTCWTFGLSKPDFKSFGIREKNGEQYLAFEFQNLMLVRDLKIRGAHNQSNALAALALGHAVGLPFDAMLASLRMFTGLEHRCQWVRNLNGVSYYNDSKATNVGATLAAIEGLSSDIDGKIVLIAGGDSKGANLKDLIKPVATHCRAVVLIGSDSNLIYATLGNAALQVRVMSLNDAIIHSRALAEPGDVVLLSPACASFDMFKNYEERGQLFTRAVEALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLQ5</accession>
    <name>A0A481QLQ5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-N-acetylglucosamine 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="8">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Enoylpyruvate transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">UDP-N-acetylglucosamine enolpyruvyl transferase</fullName>
            <shortName evidence="8">EPT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">murA</name>
        <name evidence="10" type="ORF">C3B55_00514</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="8"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00514"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="7 8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate binding" evidence="8">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-N-acetylglucosamine binding" evidence="8">
        <location>
            <begin position="122"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="2-(S-cysteinyl)pyruvic acid O-phosphothioketal" evidence="8">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QAX81848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="421" mass="45093" checksum="DE894763D486D5FD" modified="2019-06-05" version="1">MDKLIITGGARLDGEIRISGAKNAALPILAATLLCDGPVAVSNLPHLHDITTMIELFGRMGIEPVIDEKLAIEIDPRTIKTLIAPYELVKTMRASILVLGPMVARFGEAEVALPGGCAIGSRPVDLHIHGLQAMGVVINVVGGYIKAKAPKGRLRSANFFFDTVSVTGTENIMMAAALASGRSILQNTAREPEVVDLANFLIAMGAHIQGAGTDTITIDGVKCLHSASYKVMPDRIETGTYLVATAVTGGHIKIKNTDPTILEAVLEKLREAGAEIVTSKDKIELSMHGKRPKSVNVRTAPYPAFPTDMQAQFISLNAIAQGTGTVIETIFENRFMHVHELHRMGARIQVEGNTAIVTGINKLKGAPVMATDLRASASLVISALCADGDTLIDRIYHIDRGYECIEEKLQMLGAKIRRIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLS4</accession>
    <name>A0A481QLS4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Permease YicO</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">yicO</name>
        <name evidence="8" type="ORF">C3B55_00348</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00348"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904823">
        <property type="term" value="P:purine nucleobase transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005353">
        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="431" mass="45327" checksum="020A43435397BCF0" modified="2019-06-05" version="1">MLEKLFLLKAHRTNVRTEILAGLTTFLAMAYILFVNPSILSESGMDKGAVFVATCLAAAIGSTMMGLIANYPIALAPGMGLNAFFTYTVVLHMHHTWQVALGAVFISATLFFLLSIFHTREWIINSIPPPLRSAIAAGIGLFLALIALHNAGIVVSNQATMIGLGDLKQPAAILAALGFALIVALEALAIRGAILIGILAITITSITMGFTAFIGVTSMPPPLTPTFMQLDIRGALDIGMISVIFAFLFVDLFDNSGTLIGVAQRTDLISQDGYIPKISRVLIADSAAAMAGSLLGTSTTTSYIESAAGVSVGGRTGLTAIVVAILFLLALFFSPLAASIPAFASAPALLFVAVLMTSGLAEIDWNDITVAAPVTITALAMPFTYSIANGIAFGFIVWTTTKLLAGRYYELNSALVILSILFVIKLGWFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLU1</accession>
    <name>A0A481QLU1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">ftsA</name>
        <name evidence="8" type="ORF">C3B55_00573</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Cell division protein that is involved in the assembly of the Z ring. May serve as a membrane anchor for the Z ring.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Self-interacts. Interacts with FtsZ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="5">Localizes to the Z ring in an FtsZ-dependent manner. Targeted to the membrane through a conserved C-terminal amphipathic helix.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the FtsA/MreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00573"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02033">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020823">
        <property type="entry name" value="Cell_div_FtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02491">
        <property type="entry name" value="SHS2_FTSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003101">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01174">
        <property type="entry name" value="ftsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="7">
        <location>
            <begin position="10"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR003101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="QAX81906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="433" mass="46898" checksum="5F5CE1DAAC75E0DB" modified="2019-06-05" version="1">MANVQSDKMIVSLDIGTSKVVALVGEVSEISAANEITEATENSMIEIVGIGIYPSRGLKKGVVVNIESTVQSIQRAIEEAQIMAGCRIYSAFVGVAGNHIRSLNSHGIVAIRDREVSLADLERVLDAAQAVAIPADQRVLHTLPQDYVIDNQEGIREPLGMSGVRLEANVHVVTCAVNAAQNIEKCVRRCSLEIDDIILEQLASSYSVLTDDEKELGVCLVDIGGGTTDIAIFTDGAIRHTVVIPIAGDQVTNDIAMALRTPTQYAEEIKIRYACALVKLAGAGETIKVSSVGDRPPRELSRQALAEVVEPRYEELFNLIQAELRRSGYEDLIPAGIVLTGGTSKMEGVIELAEEIFQMQVRLGIPHGVNGFSDVVRNPIYSTGVGLLLYGLQKHTDGIFVTGSSMRDRYRSVSNKAKAPLFKRLQAWVKSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLV1</accession>
    <name>A0A481QLV1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Branched-chain amino acid transport system carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">brnQ</name>
        <name evidence="9" type="ORF">C3B55_00656</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the transport system for branched-chain amino acids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the branched chain amino acid transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00656"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004685">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_Livcs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30588">
        <property type="entry name" value="PTHR30588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05525">
        <property type="entry name" value="Branch_AA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00796">
        <property type="entry name" value="livcs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="437" mass="46304" checksum="758436B19536FB72" modified="2019-06-05" version="1">MTVLKSQDILALGFMTVALFVGAGNIIFPPIVGLQSGPHVWIAALGFLTTAVGMPVITVIAMARVGGGMEALSSPIGKIASSLLATATYLAVGPLFATPRTAMVSFEVGLAPLTGKNPLTLFIYSSIYFCVVYVVSLYPERLLDTVGRFLAPLKIIALVILGIAAFALPVGDVGVATQEYVTAPFSQGFMNGYLTMDTLGALVFSIVIVNAIRSRGVKSPQLITRYAIIAGLIAGVGLSLVYISLLRLGSGSYAVAAGASNGATVLHAYVQHTFGSLGSSFLAVLISLACLVTAVGLTCTCAEYFSKILPLSYKMLVMIMVLFSLFVSNLGLTKLIIFSTPVLTGIYPPCIVLVALSFFRSYWLNHSRVVSPVMLVSFVFGCIDALKSADLVDWIPTHLWLLPLSEQGSAWLVPSLITLFIAVVVDRVLGKSNRYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLV3</accession>
    <name>A0A481QLV3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Tetraacyldisaccharide 4'-kinase</fullName>
            <ecNumber evidence="3 13">2.7.1.130</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Lipid A 4'-kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">lpxK</name>
        <name evidence="14" type="ORF">C3B55_00465</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the LpxK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.130" evidence="3 13"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00465"/>
    <dbReference type="UniPathway" id="UPA00359">
        <property type="reaction ID" value="UER00482"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009029">
        <property type="term" value="F:tetraacyldisaccharide 4'-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00409">
        <property type="entry name" value="LpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003758">
        <property type="entry name" value="LpxK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42724">
        <property type="entry name" value="PTHR42724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02606">
        <property type="entry name" value="LpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00682">
        <property type="entry name" value="lpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="5 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13 14" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX81805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="318" mass="35444" checksum="A2C79A8D318CB7BB" modified="2019-06-05" version="1">MLLKPLESLYRYVVQRKRAHFLSGKLEIYKAPVPVVIVGNITVGGTGKTPLILWLIEHCRRSGLRVSVVSRGYGAKPPQLPWQVEACHSAEMVGDEPLLIVQRCGVPLMIDPDRGRAVKALLANKPTDLILSDDGLQHYGLARDLELVLVDYVRGLGNQRCLPAGPLREPIERLNSVDALLYNGANADCEDGFAFRLLPTVLINLKTGKRQPVDYLPIDQQVHAVAGIGNPQRFFNTLKMLHWNPIPHAFTNHTTYTAEILNFIPSLPLVMTEKDAVKCRAFAKSDWWYLVVDALPSPAFVSWFDTQLIRLIAASCLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLY8</accession>
    <name>A0A481QLY8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5 7">Bacterioferritin</fullName>
            <ecNumber evidence="5">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">bfr_2</name>
        <name evidence="9" type="ORF">C3B55_00617</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>4 Fe(2+) + 4 H(+) + O2 = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 7">Belongs to the bacterioferritin family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00617"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:cellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00907">
        <property type="entry name" value="Bacterioferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002024">
        <property type="entry name" value="Bacterioferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002560">
        <property type="entry name" value="Bacterioferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00601">
        <property type="entry name" value="BACFERRITIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00754">
        <property type="entry name" value="bfr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00549">
        <property type="entry name" value="BACTERIOFERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0409">Iron storage</keyword>
    <keyword evidence="5 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="8">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR002560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002560-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="156" mass="18020" checksum="89670C6B93751905" modified="2019-06-05" version="1">MKGNILVIKQLNKILANELIAINQYFLHARMYDNWGLEKLGRHEYDESIKAMKNADTLIKRLLFLEGLPNMQNLGKLNIGGHTQEIISSDLKFEHKSHSDITAAISHCETENDFNSRELLENIQKNQEEHIDWLETQLGLIIKVSLENYLQSQMCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="8" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QLZ4</accession>
    <name>A0A481QLZ4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">tRNA-modifying protein YgfZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ygfZ</name>
        <name evidence="1" type="ORF">C3B55_00731</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00731"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GcvT_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="PTHR22602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="SSF101790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="313" mass="34415" checksum="66B4A3266031AF17" modified="2019-06-05" version="1">MVDYAFFCPLTHEGVLAVRGSDSAEFLQGQLTCNLNYLSNSQASLGARCTQKGRMQSSFRILLQSDGVLLAMANGLLEPQLVDLKKYAMFSKSKLINESAAWVRFGLSNADQAMAAIGLALPIETDSVVRTDTLIAVRVSPGRAELWVSAKQADAIRNQLTNKLQQANLNDWLLGQIRAGIGQVMPQTSELFIPQMLNLQAIGGVSFKKGCYTGQEIVARMQYLGMCKRRLYRLSLSSTELPKPGTQLFSRNHNSTISKVVIAAKADQSIELLAVLHIEAANSCDVHIGTMDGPRLNLLDLPYLLDRDKEIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM25</accession>
    <name>A0A481QM25_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent zinc metalloprotease FtsH</fullName>
            <ecNumber evidence="5">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">ftsH</name>
        <name evidence="9" type="ORF">C3B55_00365</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="5"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00365"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="8">
        <location>
            <begin position="186"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="602"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="194"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX81719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="637" mass="69773" checksum="F1630137F84FD050" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="6" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QM88</accession>
    <name>A0A481QM88_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">C3B55_00640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00640"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX81965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="34" mass="3944" checksum="EE76B221F6B3506A" modified="2019-06-05" version="1">MRNAGFLLEYMMVRKKVVGLAINLVLLVLVTQFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMA5</accession>
    <name>A0A481QMA5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tagG</name>
        <name evidence="9" type="ORF">C3B55_00842</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00842"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="8">
        <location>
            <begin position="21"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="QAX82152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="272" mass="30676" checksum="BCEC51929AC50D99" modified="2019-06-05" version="1">MQHFSASPREIVGSLWRNRQLILTLIKHEVLGNFNGSIIGVLWSFITPTIMLAVYIFVFSGIFKARWNSESTSMSEFGMVLFVGLIIFNIFAECLNKGPTLILAHVNYVKKVVFPLEVLPWVSLGGALFRAVVSITVWLTAYVILFGLPHLTVLLLPFVVLPLLLLIMGLTWFLASLGVYLRNITQFVALVTTVTMFLSPIFYPANALPERFKILLLLNPLTSIIEGGRDVLFWGVVPDLQFLAIYFVVSLFIVWLGFAWFQKTRKGFADVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMA8</accession>
    <name>A0A481QMA8_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10">Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex</fullName>
            <ecNumber evidence="5 10">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">sucB</name>
        <name evidence="13" type="ORF">C3B55_00660</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA = (R)-N(6)-(S(8)-succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + CoA</text>
            <dbReference type="Rhea" id="RHEA:15213"/>
            <dbReference type="Rhea" id="RHEA-COMP:10581"/>
            <dbReference type="Rhea" id="RHEA-COMP:10582"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="2.3.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="10">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5 10"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00660"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="11">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="12">
        <location>
            <begin position="97"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QAX81985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="385" mass="41342" checksum="DBF08A3CB59712B9" modified="2019-06-05" version="1">MAIEINAPLFPESVADGTVATWHKKLGDTVKRDDLIVDIETDKVVLEVLAEADGILAAIFVEENATVLSNQVLGSIETGSAVPVADIVPVTDSEGLIAAPAARQLAEENGIKLSSIEGTGKGGRVTKEDVVAVVKTKSNALAAAMPVFAVGQRTERRVPMTRIRATVAKRLIEAQSNMAMLTTFNEVDMTSVITLRSKYKDLFIKSHNGVRLGFMSFFVKAATVALQRFSVMNAFIDGNDIVYHGYVDVGVAVSNDRGLVVPVLRNAELMSLAEIESSIAGLGKKARNGTLTIDEITGGTFTITNGGIFGSMMSTPIINPPQAAILGMHTIIQRPIAINSQVLIRPMMYLALSYDHRLIDGKDAVTFLVLIKNLLEDPARLLLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMD5</accession>
    <name>A0A481QMD5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BolA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">C3B55_00515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00515"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="SSF82657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX81849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="79" mass="9074" checksum="420DC3E143594956" modified="2019-06-05" version="1">MQILEIKNLLEGNLPETHIEVEGVGCNFQLNVISDELALLSPVKRQQQIYAHLNQWITDGRIHAVTMKFFSRAAWAKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-08-12" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMD8</accession>
    <name>A0A481QMD8_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">C3B55_00648</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX81973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00648"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="QAX81973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="353" mass="38759" checksum="169CCF1C74D743AB" modified="2019-06-05" version="1">MLKNDYLLVQMLLLVLFGANLWVMVPFWSALFWGAVLAFASWPLMRLLTYWLGGRESLAAGILTLCWMLLVALPLVWLVFNLADHVHDVVNLIKDIQVSGLPAAPTWLVLIPFVGERLVAMWDSIDQHGATLIISMKPYLGRVGNWLLARSAQVGGGILELTLSLVFVFFFYRDGPRLAMFVHRLLERLIGERADYYIELVAGTVQRVVNGVIGTAAAQAVLAFIGFLIAGVSGALVLGIVTFLLSLIPMGPPLVWVPATVWLVWNGEYTNAVFLGLWGTFIISGVDNLLKPYLISRGGNLPLVIVLLGVFGGLIAFGFIGLFIGPTLLAVAYSLLTDWSITHVEELLEDKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-01-19" version="9" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMG3</accession>
    <name>A0A481QMG3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycine cleavage system transcriptional repressor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gcvR</name>
        <name evidence="2" type="ORF">C3B55_00726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00726"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016867">
        <property type="entry name" value="GcvR"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028103">
        <property type="entry name" value="GcvR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR028103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="186" mass="20884" checksum="67E0811651D5A865" modified="2019-06-05" version="1">MFTPIVREQFLVISAIGTNPMELTNVIYRASHENRCAIVTSRLTRHGVCSALVLQVSGTWDSLARLETGLPGLTKKHDFTVNVVRSAALVSRPQALPYVAYVSSVYRSDIINKLCQFFIDHNIELENLICDTYQIPQTSGTMLNATFTLTLPIGVQISWLRDQFLDLADALNLDAMIEPWRPQNTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMH1</accession>
    <name>A0A481QMH1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00906</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00906"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="69" mass="7907" checksum="58051122CFBBF0F5" modified="2019-06-05" version="1">MVLVIASMLFTIILMLCPVNIKNGYCQHRRLVMLKCINIQYTCIAYIFIQPNSMSSNTVYLSAFSKLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMN2</accession>
    <name>A0A481QMN2_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="2">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">PreQ(0) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Queuosine biosynthesis protein QueC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">queC</name>
        <name evidence="3" type="ORF">C3B55_00973</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-deazaguanine + ADP + H(+) + H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00973"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="PTHR42914"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00364">
        <property type="entry name" value="TIGR00364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 2" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QAX82272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="233" mass="26034" checksum="2F132C71862CC96B" modified="2019-06-05" version="1">MNKKAVIVFSGGQDSTTCLIQALDIYDEVHCITFDYRQRNIAEIEVAQNLTKQLGVTVHKIIDIFSLSELASSSLTCNCIPIPTTMYSSVASFPNTFVPGRNILFLTMASIYAYQVNIKTVITGVCETDFSGYPDCRAEFIKALNKALVLGMEYKLRLDTPLMWLTKAETWALADSYQQLDLVQYKTLTCYNGIIGSGCGNCNACNLRTRGLNDYLQNSIDVMHNLKQKLEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMQ4</accession>
    <name>A0A481QMQ4_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00775"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="66" mass="7477" checksum="8C810B0DB817B8EE" modified="2019-06-05" version="1">MLILHLDNLNNCGALDKILEELTIGCKPESTFSASTTARCYNQRLLHVTLNFISIQIKNIALYLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMU7</accession>
    <name>A0A481QMU7_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L3 glutamine methyltransferase</fullName>
            <shortName evidence="4">L3 MTase</shortName>
            <ecNumber evidence="4">2.1.1.298</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">N5-glutamine methyltransferase PrmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">prmB</name>
        <name evidence="6" type="ORF">C3B55_00818</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Specifically methylates the 50S ribosomal protein L3 on a specific glutamine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-glutaminyl-[ribosomal protein uL3] + S-adenosyl-L-methionine = H(+) + N(5)-methyl-L-glutaminyl-[ribosomal protein uL3] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:45020"/>
            <dbReference type="Rhea" id="RHEA-COMP:11063"/>
            <dbReference type="Rhea" id="RHEA-COMP:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.298"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein N5-glutamine methyltransferase family. PrmB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.298" evidence="4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00818"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036009">
        <property type="term" value="F:protein-glutamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018364">
        <property type="term" value="P:peptidyl-glutamine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02125">
        <property type="entry name" value="L3_methyltr_PrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017127">
        <property type="entry name" value="Ribosome_L3_Gln-N5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037167">
        <property type="entry name" value="Mtase_YfcB_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03533">
        <property type="entry name" value="L3_gln_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="5">
        <location>
            <begin position="127"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="302" mass="34192" checksum="5E73E234C6595653" modified="2019-06-05" version="1">MIASCLRTLRDYIRWAVSCFNRENLFFGHGTDNAWDEARQLVLGALHLPWEIDDSYLDCNLEDREISHLQGLLYRRINERVPIAYLLKEAWFCGMSFIVDERVLIPRSPIGELIANYFEPWLARLPARILDLCTGSGCIGIACAYKFQDAEVVLGDLSFEALEVANQNIERHGVYERCCTVQSDGFDGMSGQHFDLIVSNPPYVDAEDFAKMPDEYHHEPKLGLACGDDGLNLVRRILAEAADHLTEKGLLIVEVGNSQMRVEALYHAVDFTWLDFQHGGYGVLMLTAEQCKEHQAMFAALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2021-09-29" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QMY1</accession>
    <name>A0A481QMY1_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase EpsM</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">epsM</name>
        <name evidence="2" type="ORF">C3B55_00850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00850"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03360">
        <property type="entry name" value="LbH_AT_putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041561">
        <property type="entry name" value="PglD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020019">
        <property type="entry name" value="Sia_OAcTrfase_NeuD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17836">
        <property type="entry name" value="PglD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03570">
        <property type="entry name" value="NeuD_NnaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PglD_N" evidence="1">
        <location>
            <begin position="7"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="QAX82160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="218" mass="23509" checksum="5650418DBACA2ACF" modified="2019-06-05" version="1">MKTFFGLIGAGGHGKEIMPMLRSMIESTLAGSNYELLFIVENLKTPTYINEYPVISLQAFFRLHGIKRFNITISNSKKRQRIAESCMAQGVLPFSLIAPSAILLDSNEIGEGVMISTQTIITSNVKIGRFFQVNNQCNISHDCIIGDFVTFGPGVRCNGNVTIGDHAYIGAGALIKQGKPEAPLKIGAGAFVGMGAVVIKDVEPYTTVVGNPARLMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN35</accession>
    <name>A0A481QN35_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="11">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">alaS</name>
        <name evidence="14" type="ORF">C3B55_00892</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="11"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00892"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 11 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 11" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II_ALA" evidence="13">
        <location>
            <begin position="1"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="722"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="560"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="663"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="667"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="QAX82198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="872" mass="95746" checksum="DBA0893B1B383B99" modified="2019-06-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-04-22" version="4" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QN97</accession>
    <name>A0A481QN97_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">C3B55_00915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">HWA_ENA</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00915"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="QAX82218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="37" mass="4507" checksum="17EBD6D763AE21C5" modified="2019-06-05" version="1">MHITKALADIVLRWLYLNEHVFLITWIDIYLQNNHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNA5</accession>
    <name>A0A481QNA5_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dCTP deaminase</fullName>
            <ecNumber evidence="3">3.5.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Deoxycytidine triphosphate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">dcd</name>
        <name evidence="5" type="ORF">C3B55_00744</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the deamination of dCTP to dUTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dCTP + H(+) + H2O = dUTP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="EC" id="3.5.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dCTP deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.13" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00744"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00665"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase" evidence="4">
        <location>
            <begin position="80"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="111"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="188" mass="21216" checksum="AC701686D6230662" modified="2019-06-05" version="1">MSIKSDKWIRRMAQEHGMIQPFVEQQVYGKGENRMISYGVSSYGYDVRCANEFKVFTNINSAIVDPKNFDENSFVDVKNDVCIIPPNSFALACTVEFFRIPRDMLIICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTTLPAKIYANEGVAQMLFLQSDEVCAVSYRDRAGKYQGQCGVTLPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2020-12-02" version="7" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNE5</accession>
    <name>A0A481QNE5_9PSED</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Electron transfer flavoprotein subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">etfB</name>
        <name evidence="4" type="ORF">C3B55_00870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00870"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="PTHR21294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ETF" evidence="3">
        <location>
            <begin position="23"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QAX82179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="249" mass="26742" checksum="5E12C36366C2ADDC" modified="2019-06-05" version="1">MKVFVAIKRVVDHNVKVRVKADHSGVDLLNVKMSMNPFCEIAVEEAIRLKEKGVATEIVVVSIGPSTAQEQLRAALALGADRAVLIEFAGELTSLATARLLKAIIDKEQPKLVILGKQAIDSDNNQTGQMLAALTGYGQGTFASKVKVNGENISVTREIDSGTQIVSLKLPAIITTDLRLNEPRYATLPNIMKAKKKLLETVTPDVLGISIASTNKTIKVEAPAVRSAGIKVKSVAELVEKLKNEAKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNF3</accession>
    <name>A0A481QNF3_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Homoserine kinase</fullName>
            <shortName evidence="3">HK</shortName>
            <shortName evidence="3">HSK</shortName>
            <ecNumber evidence="3">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">thrB</name>
        <name evidence="5" type="ORF">C3B55_00995</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pseudomonas-type ThrB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="3"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00995"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05153">
        <property type="entry name" value="HomoserineK_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00301">
        <property type="entry name" value="Homoser_kinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005280">
        <property type="entry name" value="Homoserine_kinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00938">
        <property type="entry name" value="thrB_alt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="4">
        <location>
            <begin position="31"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="QAX82293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="317" mass="35223" checksum="DA1C5FCCE6A99D39" modified="2019-06-05" version="1">MSVFTPISRLELEAFLIPYGLGRLLNFQGIISGSENTNFFISLEHGEFVLTLVERGQVNEMPFLIELLSVLHDANLPVPYVLRTVEGIALRELAGKPALLQPRLAGQHIKDANAQHCAQVGDLLGHLHLATQGKKVLEHKTDRGLDWMLAKGAQLILHLNNAPQRLLQAALSEIKDHKTAILTLPCANVHADLFRDNAMFEGTHLTGLIDFYNACSGPMLYDVAIALNDWCSHADGVIDGQRASALLNAYASLRPFTAKEAELWPTMLRVACVRFWLSRLIAAESFAGQDVLIHDPIEFEYRLAQRQQVSLHLPFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-06-05" modified="2022-08-03" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>A0A481QNG1</accession>
    <name>A0A481QNG1_9PSED</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="2">NDK</shortName>
            <shortName evidence="2">NDP kinase</shortName>
            <ecNumber evidence="2 4">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 6" type="primary">ndk</name>
        <name evidence="6" type="ORF">C3B55_00957</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Candidatus Pseudomonas adelgestsugas</name>
        <dbReference type="NCBI Taxonomy" id="1302376"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Gammaproteobacteria</taxon>
            <taxon>Pseudomonadales</taxon>
            <taxon>Pseudomonadaceae</taxon>
            <taxon>Pseudomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2018" name="Genome Biol. Evol." volume="10" first="1607" last="1621">
            <title>Partnering With a Pest: Genomes of Hemlock Woolly Adelgid Symbionts Reveal Atypical Nutritional Provisioning Patterns in Dual-Obligate Bacteria.</title>
            <authorList>
                <person name="Weglarz K.M."/>
                <person name="Havill N.P."/>
                <person name="Burke G.R."/>
                <person name="von Dohlen C.D."/>
            </authorList>
            <dbReference type="PubMed" id="29860412"/>
            <dbReference type="DOI" id=".1093/gbe/evy114"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">HWA_ENA</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 4">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="2 4"/>
    <dbReference type="EMBL" id="CP026512">
        <property type="protein sequence ID" value="QAX82258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="pade:C3B55_00957"/>
    <dbReference type="Proteomes" id="UP000288953">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006165">
        <property type="term" value="P:nucleoside diphosphate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="SSF54919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDP_KINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NDK" evidence="5">
        <location>
            <begin position="3"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="2">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="QAX82258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000288953"/>
        </source>
    </evidence>
    <sequence length="141" mass="15132" checksum="71F19B2A56E50C41" modified="2019-06-05" version="1">MAVQRTCSIIKPNAVVKNVIGEITTRFEKAGLKVIASKLTQLSKAEAEGFYAEHKARVFFGDLVTFMISSPIVVQVLEGENAIALNRELMGATNPKEAAAGTIRADFAESINANAVHGSDSEVSAAREIAYFFAVTEVNAR</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>