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<entry dataset="Swiss-Prot" created="2017-09-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F9UST1-2</accession>
    <name>LARC_LACPL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Isoform 2 of Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein</fullName>
            <shortName evidence="4">P2TMN nickel insertion protein</shortName>
            <ecNumber evidence="1 2">4.99.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Lactate racemase accessory protein LarC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Lactate racemase activation protein LarC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Lactate racemase maturation protein LarC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Lactate racemization operon protein LarC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Nickel-pincer cofactor biosynthesis protein LarC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">larC</name>
        <name evidence="5 6" type="synonym">larC1/larC2</name>
        <name evidence="5 6" type="ordered locus">lp_0106/lp_0107</name>
    </gene>
    <organism>
        <name type="scientific">Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)</name>
        <name type="synonym">Lactobacillus plantarum</name>
        <dbReference type="NCBI Taxonomy" id="220668"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Lactiplantibacillus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2003" name="Proc. Natl. Acad. Sci. U.S.A." volume="100" first="1990" last="1995">
            <title>Complete genome sequence of Lactobacillus plantarum WCFS1.</title>
            <authorList>
                <person name="Kleerebezem M."/>
                <person name="Boekhorst J."/>
                <person name="van Kranenburg R."/>
                <person name="Molenaar D."/>
                <person name="Kuipers O.P."/>
                <person name="Leer R."/>
                <person name="Tarchini R."/>
                <person name="Peters S.A."/>
                <person name="Sandbrink H.M."/>
                <person name="Fiers M.W.E.J."/>
                <person name="Stiekema W."/>
                <person name="Klein Lankhorst R.M."/>
                <person name="Bron P.A."/>
                <person name="Hoffer S.M."/>
                <person name="Nierop Groot M.N."/>
                <person name="Kerkhoven R."/>
                <person name="De Vries M."/>
                <person name="Ursing B."/>
                <person name="De Vos W.M."/>
                <person name="Siezen R.J."/>
            </authorList>
            <dbReference type="PubMed" id="12566566"/>
            <dbReference type="DOI" id="10.1073/pnas.0337704100"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC BAA-793 / NCIMB 8826 / WCFS1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Bacteriol." volume="187" first="6750" last="6761">
            <title>Lactate racemization as a rescue pathway for supplying D-lactate to the cell wall biosynthesis machinery in Lactobacillus plantarum.</title>
            <authorList>
                <person name="Goffin P."/>
                <person name="Deghorain M."/>
                <person name="Mainardi J.L."/>
                <person name="Tytgat I."/>
                <person name="Champomier-Verges M.C."/>
                <person name="Kleerebezem M."/>
                <person name="Hols P."/>
            </authorList>
            <dbReference type="PubMed" id="16166538"/>
            <dbReference type="DOI" id="10.1128/JB.187.19.6750-6761.2005"/>
        </citation>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC BAA-793 / NCIMB 8826 / WCFS1</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2012" name="J. Bacteriol." volume="194" first="195" last="196">
            <title>Complete resequencing and reannotation of the Lactobacillus plantarum WCFS1 genome.</title>
            <authorList>
                <person name="Siezen R.J."/>
                <person name="Francke C."/>
                <person name="Renckens B."/>
                <person name="Boekhorst J."/>
                <person name="Wels M."/>
                <person name="Kleerebezem M."/>
                <person name="van Hijum S.A.F.T."/>
            </authorList>
            <dbReference type="PubMed" id="22156394"/>
            <dbReference type="DOI" id="10.1128/JB.06275-11"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <scope>GENOME REANNOTATION</scope>
        <source>
            <strain>ATCC BAA-793 / NCIMB 8826 / WCFS1</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2014" name="Nat. Commun." volume="5" first="3615" last="3615">
            <title>Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system.</title>
            <authorList>
                <person name="Desguin B."/>
                <person name="Goffin P."/>
                <person name="Viaene E."/>
                <person name="Kleerebezem M."/>
                <person name="Martin-Diaconescu V."/>
                <person name="Maroney M.J."/>
                <person name="Declercq J.P."/>
                <person name="Soumillion P."/>
                <person name="Hols P."/>
            </authorList>
            <dbReference type="PubMed" id="24710389"/>
            <dbReference type="DOI" id="10.1038/NCOMMS4615"/>
        </citation>
        <scope>RIBOSOMAL FRAMESHIFT</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>NICKEL-BINDING</scope>
        <scope>INDUCTION</scope>
        <scope>DISRUPTION PHENOTYPE</scope>
        <source>
            <strain>ATCC BAA-793 / NCIMB 8826 / WCFS1</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="5598" last="5603">
            <title>Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion.</title>
            <authorList>
                <person name="Desguin B."/>
                <person name="Soumillion P."/>
                <person name="Hols P."/>
                <person name="Hausinger R.P."/>
            </authorList>
            <dbReference type="PubMed" id="27114550"/>
            <dbReference type="DOI" id="10.1073/pnas.1600486113"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <comment type="alternative products">
        <event type="ribosomal frameshifting"/>
        <isoform>
            <id>F9UST1-2</id>
            <name>2</name>
            <sequence type="described" ref="VSP_059080"/>
        </isoform>
        <isoform>
            <id>F9UST1-1</id>
            <name>1</name>
            <sequence type="displayed"/>
        </isoform>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Produced from conventional translation of the larC1 ORF. Expression of LarC1 alone does not show lactate racemase activity.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.12" evidence="1 2"/>
    <dbReference type="Proteomes" id="UP000000432">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="24710389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="27114550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="24710389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="27114550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="CCC77662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="CCC77663.1"/>
        </source>
    </evidence>
    <sequence length="263" mass="28652" checksum="15BF6BBA77C6DBC4" modified="2017-09-27" version="2">MQTLYLDAFSGISGDMFLGALLDLGLDFEQLKTELAKLHVHGYELTQQREAQSSIYGTSFDVQVAGGKDHGFVEHHHHQHEAGHHHDHEARHLADIEALIDGSDLSDTVKHHAKAIFMEIAQAEAAVHHMPLAEVHFHEVGALDSIVDIVGCCIGLELMQIDTIMASPLSDGSGFINVAHGQMPVPVPAVMQMRVGSAIPIQQRLDVHTELITPTGMGLVKTLVREFGPLPENAVPTRVGYGFGKRDTGGFNALRAVLFEKKN</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>