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<entry dataset="Swiss-Prot" created="2011-01-11" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>Q6UBL8</accession>
    <name>PB2_ISAV8</name>
    <protein>
        <recommendedName>
            <fullName>Polymerase basic protein 2</fullName>
            <shortName>PB2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein 3</fullName>
            <shortName>P3</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>RNA-directed RNA polymerase subunit P3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Segment-1</name>
    </gene>
    <organism>
        <name type="scientific">Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)</name>
        <name type="common">ISAV</name>
        <dbReference type="NCBI Taxonomy" id="652965"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Insthoviricetes</taxon>
            <taxon>Articulavirales</taxon>
            <taxon>Orthomyxoviridae</taxon>
            <taxon>Isavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oncorhynchus kisutch</name>
        <name type="common">Coho salmon</name>
        <name type="synonym">Salmo kisutch</name>
        <dbReference type="NCBI Taxonomy" id="8019"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Oncorhynchus mykiss</name>
        <name type="common">Rainbow trout</name>
        <name type="synonym">Salmo gairdneri</name>
        <dbReference type="NCBI Taxonomy" id="8022"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo salar</name>
        <name type="common">Atlantic salmon</name>
        <dbReference type="NCBI Taxonomy" id="8030"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo trutta</name>
        <name type="common">Brown trout</name>
        <dbReference type="NCBI Taxonomy" id="8032"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pollachius virens</name>
        <name type="common">Saithe</name>
        <name type="synonym">Gadus virens</name>
        <dbReference type="NCBI Taxonomy" id="8060"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Gadus morhua</name>
        <name type="common">Atlantic cod</name>
        <dbReference type="NCBI Taxonomy" id="8049"/>
    </organismHost>
    <reference key="1">
        <citation type="submission" date="2003-08" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Kibenge F.S."/>
                <person name="Qian B."/>
                <person name="Kibenge M.J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Virus Res." volume="92" first="99" last="105">
            <title>Isolation and characterisation of segment 1 of the infectious salmon anaemia virus genome.</title>
            <authorList>
                <person name="Snow M."/>
                <person name="Ritchie R."/>
                <person name="Arnaud O."/>
                <person name="Villoing S."/>
                <person name="Aspehaug V."/>
                <person name="Cunningham C.O."/>
            </authorList>
            <dbReference type="PubMed" id="12606081"/>
            <dbReference type="DOI" id="10.1016/S0168-1702(02)00322-2"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="Virus Res." volume="155" first="10" last="19">
            <title>Infectious salmon anemia virus--genetics and pathogenesis.</title>
            <authorList>
                <person name="Cottet L."/>
                <person name="Rivas-Aravena A."/>
                <person name="Cortez-San Martin M."/>
                <person name="Sandino A.M."/>
                <person name="Spencer E."/>
            </authorList>
            <dbReference type="PubMed" id="20979983"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.10.021"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNA polymerase is composed of three subunits: PB1, PB2 and PA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY373381">
        <property type="protein sequence ID" value="AAQ81914.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145807.1">
        <property type="nucleotide sequence ID" value="NC_006505.1"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6UBL8"/>
    <dbReference type="GeneID" id="3170818"/>
    <dbReference type="KEGG" id="vg:3170818"/>
    <dbReference type="Proteomes" id="UP000008772">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000403917" description="Polymerase basic protein 2">
        <location>
            <begin position="1"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12606081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="20979983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="722" mass="79918" checksum="DAB5408D3035ECD0" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2022-02-23" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8V3T6</accession>
    <name>RDRP_ISAV8</name>
    <protein>
        <recommendedName>
            <fullName>RNA-directed RNA polymerase catalytic subunit</fullName>
            <ecNumber>2.7.7.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Polymerase basic protein 1</fullName>
            <shortName>PB1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein 1</fullName>
            <shortName>P1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>RNA-directed RNA polymerase subunit P1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Segment-2</name>
    </gene>
    <organism>
        <name type="scientific">Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)</name>
        <name type="common">ISAV</name>
        <dbReference type="NCBI Taxonomy" id="652965"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Insthoviricetes</taxon>
            <taxon>Articulavirales</taxon>
            <taxon>Orthomyxoviridae</taxon>
            <taxon>Isavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oncorhynchus kisutch</name>
        <name type="common">Coho salmon</name>
        <name type="synonym">Salmo kisutch</name>
        <dbReference type="NCBI Taxonomy" id="8019"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Oncorhynchus mykiss</name>
        <name type="common">Rainbow trout</name>
        <name type="synonym">Salmo gairdneri</name>
        <dbReference type="NCBI Taxonomy" id="8022"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo salar</name>
        <name type="common">Atlantic salmon</name>
        <dbReference type="NCBI Taxonomy" id="8030"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo trutta</name>
        <name type="common">Brown trout</name>
        <dbReference type="NCBI Taxonomy" id="8032"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pollachius virens</name>
        <name type="common">Saithe</name>
        <name type="synonym">Gadus virens</name>
        <dbReference type="NCBI Taxonomy" id="8060"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Gadus morhua</name>
        <name type="common">Atlantic cod</name>
        <dbReference type="NCBI Taxonomy" id="8049"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="J. Gen. Virol." volume="83" first="421" last="428">
            <title>Genomic organization of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="Clouthier S.C."/>
                <person name="Rector T."/>
                <person name="Brown N.E."/>
                <person name="Anderson E.D."/>
            </authorList>
            <dbReference type="PubMed" id="11807235"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2006-06" db="EMBL/GenBank/DDBJ databases">
            <title>Infectious salmon anaemia virus genomic RNA segment 2.</title>
            <authorList>
                <person name="Kibenge F.S."/>
                <person name="Kibenge M.J."/>
                <person name="Qian B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
        <source>
            <strain>RPC/NB 02-0775-14</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="Virus Res." volume="155" first="10" last="19">
            <title>Infectious salmon anemia virus--genetics and pathogenesis.</title>
            <authorList>
                <person name="Cottet L."/>
                <person name="Rivas-Aravena A."/>
                <person name="Cortez-San Martin M."/>
                <person name="Sandino A.M."/>
                <person name="Spencer E."/>
            </authorList>
            <dbReference type="PubMed" id="20979983"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.10.021"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <comment type="function">
        <text evidence="1">RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNA polymerase is composed of three subunits: PB1, PB2 and PA.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48"/>
    <dbReference type="EMBL" id="AF404346">
        <property type="protein sequence ID" value="AAL67962.1"/>
        <property type="molecule type" value="Viral_cRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DQ676937">
        <property type="protein sequence ID" value="ABG81414.1"/>
        <property type="molecule type" value="Viral_cRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145804.1">
        <property type="nucleotide sequence ID" value="NC_006503.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q8V3T6"/>
    <dbReference type="PRIDE" id="Q8V3T6"/>
    <dbReference type="GeneID" id="3170817"/>
    <dbReference type="KEGG" id="vg:3170817"/>
    <dbReference type="Proteomes" id="UP000008772">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007099">
        <property type="entry name" value="RNA-dir_pol_NSvirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001407">
        <property type="entry name" value="RNA_pol_PB1_influenza"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00602">
        <property type="entry name" value="Flu_PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000827">
        <property type="entry name" value="RdRPol_OMV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50525">
        <property type="entry name" value="RDRP_SSRNA_NEG_SEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0693">Viral RNA replication</keyword>
    <feature type="chain" id="PRO_0000403918" description="RNA-directed RNA polymerase catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="RdRp catalytic" evidence="2">
        <location>
            <begin position="281"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="20979983"/>
        </source>
    </evidence>
    <sequence length="708" mass="80526" checksum="3D4128B467CC899B" modified="2002-03-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8V3T7</accession>
    <name>NCAP_ISAV8</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleoprotein</fullName>
            <shortName>NP</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Nucleocapsid protein</fullName>
            <shortName>Protein N</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>vp66</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Segment-3</name>
    </gene>
    <organism>
        <name type="scientific">Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)</name>
        <name type="common">ISAV</name>
        <dbReference type="NCBI Taxonomy" id="652965"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Insthoviricetes</taxon>
            <taxon>Articulavirales</taxon>
            <taxon>Orthomyxoviridae</taxon>
            <taxon>Isavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oncorhynchus kisutch</name>
        <name type="common">Coho salmon</name>
        <name type="synonym">Salmo kisutch</name>
        <dbReference type="NCBI Taxonomy" id="8019"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Oncorhynchus mykiss</name>
        <name type="common">Rainbow trout</name>
        <name type="synonym">Salmo gairdneri</name>
        <dbReference type="NCBI Taxonomy" id="8022"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo salar</name>
        <name type="common">Atlantic salmon</name>
        <dbReference type="NCBI Taxonomy" id="8030"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo trutta</name>
        <name type="common">Brown trout</name>
        <dbReference type="NCBI Taxonomy" id="8032"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pollachius virens</name>
        <name type="common">Saithe</name>
        <name type="synonym">Gadus virens</name>
        <dbReference type="NCBI Taxonomy" id="8060"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Gadus morhua</name>
        <name type="common">Atlantic cod</name>
        <dbReference type="NCBI Taxonomy" id="8049"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="J. Gen. Virol." volume="83" first="421" last="428">
            <title>Genomic organization of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="Clouthier S.C."/>
                <person name="Rector T."/>
                <person name="Brown N.E."/>
                <person name="Anderson E.D."/>
            </authorList>
            <dbReference type="PubMed" id="11807235"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Virus Res." volume="106" first="51" last="60">
            <title>Infectious salmon anemia virus (ISAV) genomic segment 3 encodes the viral nucleoprotein (NP), an RNA-binding protein with two monopartite nuclear localization signals (NLS).</title>
            <authorList>
                <person name="Aspehaug V."/>
                <person name="Falk K."/>
                <person name="Krossoy B."/>
                <person name="Thevarajan J."/>
                <person name="Sanders L."/>
                <person name="Moore L."/>
                <person name="Endresen C."/>
                <person name="Biering E."/>
            </authorList>
            <dbReference type="PubMed" id="15522447"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2004.06.001"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2004" name="J. Virol." volume="78" first="3063" last="3071">
            <title>Identification and characterization of viral structural proteins of infectious salmon anemia virus.</title>
            <authorList>
                <person name="Falk K."/>
                <person name="Aspehaug V."/>
                <person name="Vlasak R."/>
                <person name="Endresen C."/>
            </authorList>
            <dbReference type="PubMed" id="14990725"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2008" name="Virology" volume="379" first="55" last="63">
            <title>The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells.</title>
            <authorList>
                <person name="Goic B."/>
                <person name="Bustamante J."/>
                <person name="Miquel A."/>
                <person name="Alvarez M."/>
                <person name="Vera M.I."/>
                <person name="Valenzuela P.D."/>
                <person name="Burzio L.O."/>
            </authorList>
            <dbReference type="PubMed" id="18632128"/>
            <dbReference type="DOI" id="10.1016/j.virol.2008.05.036"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2011" name="Virus Res." volume="155" first="10" last="19">
            <title>Infectious salmon anemia virus--genetics and pathogenesis.</title>
            <authorList>
                <person name="Cottet L."/>
                <person name="Rivas-Aravena A."/>
                <person name="Cortez-San Martin M."/>
                <person name="Sandino A.M."/>
                <person name="Spencer E."/>
            </authorList>
            <dbReference type="PubMed" id="20979983"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.10.021"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <comment type="function">
        <text evidence="3 7">Encapsidates the negative strand viral RNA, protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication (PubMed:15522447).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host nucleus</location>
            <location evidence="4">Host nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AF404345">
        <property type="protein sequence ID" value="AAL67961.1"/>
        <property type="molecule type" value="Viral_cRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145803.1">
        <property type="nucleotide sequence ID" value="NC_006502.1"/>
    </dbReference>
    <dbReference type="PDB" id="4EWC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A=2-600"/>
    </dbReference>
    <dbReference type="PDBsum" id="4EWC"/>
    <dbReference type="SMR" id="Q8V3T7"/>
    <dbReference type="GeneID" id="3170816"/>
    <dbReference type="KEGG" id="vg:3170816"/>
    <dbReference type="Proteomes" id="UP000008772">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1139">Helical capsid protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0543">Viral nucleoprotein</keyword>
    <keyword id="KW-1163">Viral penetration into host nucleus</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="chain" id="PRO_0000403915" description="Nucleoprotein">
        <location>
            <begin position="1"/>
            <end position="616"/>
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</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>Q8V3T8</accession>
    <name>PA_ISAV8</name>
    <protein>
        <recommendedName>
            <fullName>Polymerase acidic protein</fullName>
            <shortName>PA</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein 2</fullName>
            <shortName>P2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>RNA-directed RNA polymerase subunit P2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Segment-4</name>
    </gene>
    <organism>
        <name type="scientific">Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)</name>
        <name type="common">ISAV</name>
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        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Insthoviricetes</taxon>
            <taxon>Articulavirales</taxon>
            <taxon>Orthomyxoviridae</taxon>
            <taxon>Isavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oncorhynchus kisutch</name>
        <name type="common">Coho salmon</name>
        <name type="synonym">Salmo kisutch</name>
        <dbReference type="NCBI Taxonomy" id="8019"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Oncorhynchus mykiss</name>
        <name type="common">Rainbow trout</name>
        <name type="synonym">Salmo gairdneri</name>
        <dbReference type="NCBI Taxonomy" id="8022"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo salar</name>
        <name type="common">Atlantic salmon</name>
        <dbReference type="NCBI Taxonomy" id="8030"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo trutta</name>
        <name type="common">Brown trout</name>
        <dbReference type="NCBI Taxonomy" id="8032"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pollachius virens</name>
        <name type="common">Saithe</name>
        <name type="synonym">Gadus virens</name>
        <dbReference type="NCBI Taxonomy" id="8060"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Gadus morhua</name>
        <name type="common">Atlantic cod</name>
        <dbReference type="NCBI Taxonomy" id="8049"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="J. Gen. Virol." volume="83" first="421" last="428">
            <title>Genomic organization of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="Clouthier S.C."/>
                <person name="Rector T."/>
                <person name="Brown N.E."/>
                <person name="Anderson E.D."/>
            </authorList>
            <dbReference type="PubMed" id="11807235"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Virus Res." volume="155" first="10" last="19">
            <title>Infectious salmon anemia virus--genetics and pathogenesis.</title>
            <authorList>
                <person name="Cottet L."/>
                <person name="Rivas-Aravena A."/>
                <person name="Cortez-San Martin M."/>
                <person name="Sandino A.M."/>
                <person name="Spencer E."/>
            </authorList>
            <dbReference type="PubMed" id="20979983"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.10.021"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Implicated in endonuclease cleavage of capped RNA primers. Displays an elongation factor activity in viral RNA synthesis. Dispensable for viral transcription, but not replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNA polymerase is composed of three subunits: PB1, PB2 and PA.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated on serines and threonines by host kinases.</text>
    </comment>
    <dbReference type="EMBL" id="AF404344">
        <property type="protein sequence ID" value="AAL67960.1"/>
        <property type="molecule type" value="Viral_cRNA"/>
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        <property type="nucleotide sequence ID" value="NC_006501.1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="578"/>
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        </source>
    </evidence>
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</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8V3T9</accession>
    <name>FUS_ISAV8</name>
    <protein>
        <recommendedName>
            <fullName>Fusion glycoprotein F0</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein 3</fullName>
            <shortName>P3</shortName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Fusion glycoprotein F1</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Fusion glycoprotein F2</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="7" type="primary">Segment-5</name>
    </gene>
    <organism>
        <name type="scientific">Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)</name>
        <name type="common">ISAV</name>
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        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Insthoviricetes</taxon>
            <taxon>Articulavirales</taxon>
            <taxon>Orthomyxoviridae</taxon>
            <taxon>Isavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oncorhynchus kisutch</name>
        <name type="common">Coho salmon</name>
        <name type="synonym">Salmo kisutch</name>
        <dbReference type="NCBI Taxonomy" id="8019"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Oncorhynchus mykiss</name>
        <name type="common">Rainbow trout</name>
        <name type="synonym">Salmo gairdneri</name>
        <dbReference type="NCBI Taxonomy" id="8022"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo salar</name>
        <name type="common">Atlantic salmon</name>
        <dbReference type="NCBI Taxonomy" id="8030"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo trutta</name>
        <name type="common">Brown trout</name>
        <dbReference type="NCBI Taxonomy" id="8032"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pollachius virens</name>
        <name type="common">Saithe</name>
        <name type="synonym">Gadus virens</name>
        <dbReference type="NCBI Taxonomy" id="8060"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Gadus morhua</name>
        <name type="common">Atlantic cod</name>
        <dbReference type="NCBI Taxonomy" id="8049"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="J. Gen. Virol." volume="83" first="421" last="428">
            <title>Genomic organization of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="Clouthier S.C."/>
                <person name="Rector T."/>
                <person name="Brown N.E."/>
                <person name="Anderson E.D."/>
            </authorList>
            <dbReference type="PubMed" id="11807235"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Virol." volume="79" first="12544" last="12553">
            <title>Characterization of the infectious salmon anemia virus fusion protein.</title>
            <authorList>
                <person name="Aspehaug V."/>
                <person name="Mikalsen A.B."/>
                <person name="Snow M."/>
                <person name="Biering E."/>
                <person name="Villoing S."/>
            </authorList>
            <dbReference type="PubMed" id="16160182"/>
            <dbReference type="DOI" id="10.1128/JVI.79.19.12544-12553.2005"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2004" name="J. Virol." volume="78" first="3063" last="3071">
            <title>Identification and characterization of viral structural proteins of infectious salmon anemia virus.</title>
            <authorList>
                <person name="Falk K."/>
                <person name="Aspehaug V."/>
                <person name="Vlasak R."/>
                <person name="Endresen C."/>
            </authorList>
            <dbReference type="PubMed" id="14990725"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2011" name="Virus Res." volume="155" first="10" last="19">
            <title>Infectious salmon anemia virus--genetics and pathogenesis.</title>
            <authorList>
                <person name="Cottet L."/>
                <person name="Rivas-Aravena A."/>
                <person name="Cortez-San Martin M."/>
                <person name="Sandino A.M."/>
                <person name="Spencer E."/>
            </authorList>
            <dbReference type="PubMed" id="20979983"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.10.021"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2014" name="J. Gen. Virol." volume="95" first="1015" last="1024">
            <title>Deletions in the highly polymorphic region (HPR) of infectious salmon anaemia virus HPR0 haemagglutinin-esterase enhance viral fusion and influence the interaction with the fusion protein.</title>
            <authorList>
                <person name="Fourrier M."/>
                <person name="Lester K."/>
                <person name="Thoen E."/>
                <person name="Mikalsen A."/>
                <person name="Evensen O."/>
                <person name="Falk K."/>
                <person name="Collet B."/>
                <person name="McBeath A."/>
            </authorList>
            <dbReference type="PubMed" id="24486627"/>
            <dbReference type="DOI" id="10.1099/vir.0.061648-0"/>
        </citation>
        <scope>INTERACTION WITH HEMAGGLUTININ ESTERASE</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2015" name="J. Biol. Chem." volume="290" first="18495" last="18504">
            <title>Electrostatic Architecture of the Infectious Salmon Anemia Virus (ISAV) Core Fusion Protein Illustrates a Carboxyl-Carboxylate pH Sensor.</title>
            <authorList>
                <person name="Cook J.D."/>
                <person name="Soto-Montoya H."/>
                <person name="Korpela M.K."/>
                <person name="Lee J.E."/>
            </authorList>
            <dbReference type="PubMed" id="26082488"/>
            <dbReference type="DOI" id="10.1074/jbc.M115.644781"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="4 6">Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. The trimer of F1-F2 (F protein) probably interacts with HE at the virion surface. Upon HE binding to its cellular receptor, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between cell and virion membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homotrimer of disulfide-linked F1-F2 (PubMed:16160182). Interacts with Hemagglutinin esterase (PubMed:24486627).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Virion membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Host membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="4">The inactive precursor F0 is glycosylated and proteolytically cleaved into F1 and F2 to be functionally active. The cleavage is mediated by cellular proteases during the transport and maturation of the polypeptide.</text>
    </comment>
    <dbReference type="EMBL" id="AF404343">
        <property type="protein sequence ID" value="AAL67959.1"/>
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        <property type="nucleotide sequence ID" value="NC_006500.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q8V3T9"/>
    <dbReference type="GeneID" id="3170815"/>
    <dbReference type="KEGG" id="vg:3170815"/>
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        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025167">
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    <dbReference type="Pfam" id="PF13044">
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    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403919" description="Fusion glycoprotein F0">
        <location>
            <begin position="18"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403920" description="Fusion glycoprotein F1">
        <location>
            <begin position="18"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403921" description="Fusion glycoprotein F2">
        <location>
            <begin position="277"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="18"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fusion peptide" evidence="1">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by host" evidence="1">
        <location>
            <begin position="276"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="14990725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="16160182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="24486627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="26082488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="7">
        <source>
            <dbReference type="PubMed" id="20979983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="8"/>
    <sequence length="444" mass="48773" checksum="388C3090B65C8D41" modified="2002-03-01" version="1" precursor="true">MAFLTILVLFLFKEVLCEPCICENPTCLGITIPQAGFVRSAPGGVLLTETITERPQLTEWTTSRPKLEETLWLDGETKNGKVSQTLFEAIQGTQMENCAVKAVLDTTFVNLTKQDIVLGKIKVSEFGGDSDISKCGRKGLKVFICGGTVGYVTRGCPPEECKGKKGRMMALEPTTDCGVEKGLTTDRIKTGMLDITSCCTQHGCTKGIRVEVPSPVLVSSKCQEVTFRVVPFHSVPDKLGFARTSSFTLKANFVNKHGWSKYNFNLRGFPGEEFIKCCGFTLGVGGAWFQAYLNGMVQGDGAASADDVKEKLNGIIDQINKANTLLEGEIEAVRRIAYMNQASSLQNQVEIGLIGEYLNISSWLETTTLTKTEEGLMKNGWCQSNTHCWCPPKPTIVPTIGYVDSIKEVTGTSWWMVMIHYIIVGLIVIVVVVFGLKLWGCLRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8V3U0</accession>
    <name>HEMA_ISAV8</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hemagglutinin-esterase</fullName>
            <shortName>HE</shortName>
            <ecNumber>3.1.1.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>gp42</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Segment-6</name>
    </gene>
    <organism>
        <name type="scientific">Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)</name>
        <name type="common">ISAV</name>
        <dbReference type="NCBI Taxonomy" id="652965"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Insthoviricetes</taxon>
            <taxon>Articulavirales</taxon>
            <taxon>Orthomyxoviridae</taxon>
            <taxon>Isavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oncorhynchus kisutch</name>
        <name type="common">Coho salmon</name>
        <name type="synonym">Salmo kisutch</name>
        <dbReference type="NCBI Taxonomy" id="8019"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Oncorhynchus mykiss</name>
        <name type="common">Rainbow trout</name>
        <name type="synonym">Salmo gairdneri</name>
        <dbReference type="NCBI Taxonomy" id="8022"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo salar</name>
        <name type="common">Atlantic salmon</name>
        <dbReference type="NCBI Taxonomy" id="8030"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo trutta</name>
        <name type="common">Brown trout</name>
        <dbReference type="NCBI Taxonomy" id="8032"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pollachius virens</name>
        <name type="common">Saithe</name>
        <name type="synonym">Gadus virens</name>
        <dbReference type="NCBI Taxonomy" id="8060"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Gadus morhua</name>
        <name type="common">Atlantic cod</name>
        <dbReference type="NCBI Taxonomy" id="8049"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="J. Gen. Virol." volume="83" first="421" last="428">
            <title>Genomic organization of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="Clouthier S.C."/>
                <person name="Rector T."/>
                <person name="Brown N.E."/>
                <person name="Anderson E.D."/>
            </authorList>
            <dbReference type="PubMed" id="11807235"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Virol." volume="78" first="3055" last="3062">
            <title>Infectious salmon anemia virus specifically binds to and hydrolyzes 4-O-acetylated sialic acids.</title>
            <authorList>
                <person name="Hellebo A."/>
                <person name="Vilas U."/>
                <person name="Falk K."/>
                <person name="Vlasak R."/>
            </authorList>
            <dbReference type="PubMed" id="14990724"/>
            <dbReference type="DOI" id="10.1128/JVI.78.6.3055-3062.2004"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2004" name="J. Virol." volume="78" first="3063" last="3071">
            <title>Identification and characterization of viral structural proteins of infectious salmon anemia virus.</title>
            <authorList>
                <person name="Falk K."/>
                <person name="Aspehaug V."/>
                <person name="Vlasak R."/>
                <person name="Endresen C."/>
            </authorList>
            <dbReference type="PubMed" id="14990725"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="Arch. Virol." volume="150" first="1621" last="1637">
            <title>Expression, antigenicity and studies on cell receptor binding of the hemagglutinin of infectious salmon anemia virus.</title>
            <authorList>
                <person name="Mikalsen A.B."/>
                <person name="Sindre H."/>
                <person name="Mjaaland S."/>
                <person name="Rimstad E."/>
            </authorList>
            <dbReference type="PubMed" id="15824888"/>
            <dbReference type="DOI" id="10.1007/s00705-005-0502-4"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2010" name="Virus Res." volume="151" first="131" last="141">
            <title>Structural and functional analysis of the hemagglutinin-esterase of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="Mueller A."/>
                <person name="Markussen T."/>
                <person name="Drabloes F."/>
                <person name="Gjoeen T."/>
                <person name="Joergensen T.O."/>
                <person name="Solem S.T."/>
                <person name="Mjaaland S."/>
            </authorList>
            <dbReference type="PubMed" id="20398710"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.03.020"/>
        </citation>
        <scope>ACTIVE SITE</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2011" name="Virus Res." volume="155" first="10" last="19">
            <title>Infectious salmon anemia virus--genetics and pathogenesis.</title>
            <authorList>
                <person name="Cottet L."/>
                <person name="Rivas-Aravena A."/>
                <person name="Cortez-San Martin M."/>
                <person name="Sandino A.M."/>
                <person name="Spencer E."/>
            </authorList>
            <dbReference type="PubMed" id="20979983"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.10.021"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2012" name="J. Virol." volume="86" first="10571" last="10578">
            <title>Expression of the infectious salmon anemia virus receptor on atlantic salmon endothelial cells correlates with the cell tropism of the virus.</title>
            <authorList>
                <person name="Aamelfot M."/>
                <person name="Dale O.B."/>
                <person name="Weli S.C."/>
                <person name="Koppang E.O."/>
                <person name="Falk K."/>
            </authorList>
            <dbReference type="PubMed" id="22811536"/>
            <dbReference type="DOI" id="10.1128/JVI.00047-12"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2014" name="J. Gen. Virol." volume="95" first="1015" last="1024">
            <title>Deletions in the highly polymorphic region (HPR) of infectious salmon anaemia virus HPR0 haemagglutinin-esterase enhance viral fusion and influence the interaction with the fusion protein.</title>
            <authorList>
                <person name="Fourrier M."/>
                <person name="Lester K."/>
                <person name="Thoen E."/>
                <person name="Mikalsen A."/>
                <person name="Evensen O."/>
                <person name="Falk K."/>
                <person name="Collet B."/>
                <person name="McBeath A."/>
            </authorList>
            <dbReference type="PubMed" id="24486627"/>
            <dbReference type="DOI" id="10.1099/vir.0.061648-0"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 4 5">Performs attachment to host receptor thereby inducing virus particle entry into target cell. Binds specifically to 5-N-acetyl-4-O-acetyl neuraminic acid on host cells, which plays a major role in cell tropism of the virus (PubMed:14990724, PubMed:22811536). ALso mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid (PubMed:14990724). This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell (PubMed:14990724). The highly polymorphic region (HPR) modulates the virulence in host (PubMed:24486627). Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates (PubMed:14990724).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-acetyl-9-O-acetylneuraminate = acetate + H(+) + N-acetylneuraminate</text>
            <dbReference type="Rhea" id="RHEA:22600"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28999"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35418"/>
            <dbReference type="EC" id="3.1.1.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-acetyl-4-O-acetylneuraminate = acetate + H(+) + N-acetylneuraminate</text>
            <dbReference type="Rhea" id="RHEA:25564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29006"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35418"/>
            <dbReference type="EC" id="3.1.1.53"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Host membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 9">Virion membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.1.53"/>
    <dbReference type="EMBL" id="AF404342">
        <property type="protein sequence ID" value="AAL67958.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145800.1">
        <property type="nucleotide sequence ID" value="NC_006499.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q8V3U0"/>
    <dbReference type="GeneID" id="3170814"/>
    <dbReference type="KEGG" id="vg:3170814"/>
    <dbReference type="Proteomes" id="UP000008772">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033644">
        <property type="term" value="C:host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106331">
        <property type="term" value="F:sialate 4-O-acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106330">
        <property type="term" value="F:sialate 9-O-acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039654">
        <property type="term" value="P:fusion of virus membrane with host endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046718">
        <property type="term" value="P:viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019062">
        <property type="term" value="P:virion attachment to host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010408">
        <property type="entry name" value="Hemagglutn-estrase_ISAV-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06215">
        <property type="entry name" value="ISAV_HA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1170">Fusion of virus membrane with host endosomal membrane</keyword>
    <keyword id="KW-1168">Fusion of virus membrane with host membrane</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403914" description="Hemagglutinin-esterase">
        <location>
            <begin position="17"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="17"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Highly polymorphic region" evidence="8">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cell attachment site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="14990724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="14990725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="22811536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="24486627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="14990725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="7">
        <source>
            <dbReference type="PubMed" id="20979983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="8">
        <source>
            <dbReference type="PubMed" id="24486627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="9">
        <source>
            <dbReference type="PubMed" id="15824888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="10">
        <source>
            <dbReference type="PubMed" id="20398710"/>
        </source>
    </evidence>
    <sequence length="394" mass="43081" checksum="7E169F87E7FAEEA4" modified="2002-03-01" version="1" precursor="true">MARFIILFLLLAPVYSRLCLRNHPDTTWIGDSRSDQSRVNQQSLDLVTNFKGILQAKNGNGLMKQMSGRFPSDWYQPTTKYRILYIGTNDCTEGPNDVIIPTSMTLDNVARDLYLGACRGDVRVTPTFVGAAELGLIGRTDALTEFSVKVLTFNNPTIVVVGLNGMSGIYKVCIAASSGNVGGVNLVNGCGYFSAPLRFDNFKGQIYVSDTFEVRGTKNKCVILRSSSNAPLCTHIKRNIELDEYVDTPNTGGVYPSDGFDSLHGSASIRTFLTEALTCPGVDWDRIDAASCEYDSCPKLVKEFDQTGLGNTDTQIMRELEAQKEMIGKLGRNITDVNNRVDAIPPQLSNIFISMGVAGFGIALFLAGWKACVWIAAFMYKSRGRNPPANLSVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2022-02-23" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8V3U1</accession>
    <name>NEP_ISAV8</name>
    <protein>
        <recommendedName>
            <fullName>Nuclear export protein</fullName>
            <shortName>NEP</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P5</fullName>
            <shortName>P5</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Segment-7</name>
        <name type="ORF">s7ORF2</name>
    </gene>
    <organism>
        <name type="scientific">Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)</name>
        <name type="common">ISAV</name>
        <dbReference type="NCBI Taxonomy" id="652965"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Insthoviricetes</taxon>
            <taxon>Articulavirales</taxon>
            <taxon>Orthomyxoviridae</taxon>
            <taxon>Isavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oncorhynchus kisutch</name>
        <name type="common">Coho salmon</name>
        <name type="synonym">Salmo kisutch</name>
        <dbReference type="NCBI Taxonomy" id="8019"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Oncorhynchus mykiss</name>
        <name type="common">Rainbow trout</name>
        <name type="synonym">Salmo gairdneri</name>
        <dbReference type="NCBI Taxonomy" id="8022"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo salar</name>
        <name type="common">Atlantic salmon</name>
        <dbReference type="NCBI Taxonomy" id="8030"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo trutta</name>
        <name type="common">Brown trout</name>
        <dbReference type="NCBI Taxonomy" id="8032"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pollachius virens</name>
        <name type="common">Saithe</name>
        <name type="synonym">Gadus virens</name>
        <dbReference type="NCBI Taxonomy" id="8060"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Gadus morhua</name>
        <name type="common">Atlantic cod</name>
        <dbReference type="NCBI Taxonomy" id="8049"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="J. Gen. Virol." volume="83" first="421" last="428">
            <title>Genomic organization of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="Clouthier S.C."/>
                <person name="Rector T."/>
                <person name="Brown N.E."/>
                <person name="Anderson E.D."/>
            </authorList>
            <dbReference type="PubMed" id="11807235"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Virus Res." volume="155" first="10" last="19">
            <title>Infectious salmon anemia virus--genetics and pathogenesis.</title>
            <authorList>
                <person name="Cottet L."/>
                <person name="Rivas-Aravena A."/>
                <person name="Cortez-San Martin M."/>
                <person name="Sandino A.M."/>
                <person name="Spencer E."/>
            </authorList>
            <dbReference type="PubMed" id="20979983"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.10.021"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2013" name="Virus Res." volume="177" first="1" last="10">
            <title>Infectious salmon anaemia virus nuclear export protein is encoded by a spliced gene product of genomic segment 7.</title>
            <authorList>
                <person name="Ramly R.B."/>
                <person name="Olsen C.M."/>
                <person name="Braaen S."/>
                <person name="Rimstad E."/>
            </authorList>
            <dbReference type="PubMed" id="23850870"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2013.07.001"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ALTERNATIVE SPLICING</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2017" name="Virol. J." volume="14" first="30" last="30">
            <title>Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70.</title>
            <authorList>
                <person name="Zhang W."/>
                <person name="Cai C."/>
                <person name="Lin L."/>
                <person name="Tao Y.J."/>
                <person name="Jin M."/>
            </authorList>
            <dbReference type="PubMed" id="28202040"/>
            <dbReference type="DOI" id="10.1186/s12985-017-0702-z"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INTERACTION WITH HOST HSC70</scope>
    </reference>
    <comment type="function">
        <text evidence="4 5">May mediate the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs) (PubMed:23850870, PubMed:28202040). Interaction of viral NEP with M1-Hsc70 is thought to promote nuclear export of the viral encapsidated genomes (PubMed:28202040).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with host HSC70.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="alternative splicing"/>
        <isoform>
            <id>Q8V3U1-1</id>
            <name evidence="1">Nuclear export protein</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q8V3U2-1</id>
            <name>Non-structural protein 1</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <dbReference type="EMBL" id="AF404341">
        <property type="protein sequence ID" value="AAL67957.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145799.1">
        <property type="nucleotide sequence ID" value="NC_006498.1"/>
    </dbReference>
    <dbReference type="GeneID" id="5075849"/>
    <dbReference type="KEGG" id="vg:5075849"/>
    <dbReference type="Proteomes" id="UP000008772">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0025">Alternative splicing</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000403923" description="Nuclear export protein">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="23850870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="28202040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="20979983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="23850870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="28202040"/>
        </source>
    </evidence>
    <sequence length="146" mass="16268" checksum="A4616D7BBB30C89F" modified="2002-03-01" version="1">MNLLLLLQVASFLSDSKVPGEDGTSSTSGMLDLLRDQVDSLSINDSTTEPKTRLDPGLYPWLKWTETAYRSSTRSLASTIVMGALVQQRGSGNGITMRELELSLGLDFTSECDWLKTCYVNKNFVFLSEKEIAVNMEVEKFICNEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>Q8V3U2</accession>
    <name>NS1_ISAV8</name>
    <protein>
        <recommendedName>
            <fullName>Non-structural protein 1</fullName>
            <shortName>NS1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P4</fullName>
            <shortName>P4</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Segment-7</name>
        <name type="ORF">s7ORF1</name>
    </gene>
    <organism>
        <name type="scientific">Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)</name>
        <name type="common">ISAV</name>
        <dbReference type="NCBI Taxonomy" id="652965"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Insthoviricetes</taxon>
            <taxon>Articulavirales</taxon>
            <taxon>Orthomyxoviridae</taxon>
            <taxon>Isavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oncorhynchus kisutch</name>
        <name type="common">Coho salmon</name>
        <name type="synonym">Salmo kisutch</name>
        <dbReference type="NCBI Taxonomy" id="8019"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Oncorhynchus mykiss</name>
        <name type="common">Rainbow trout</name>
        <name type="synonym">Salmo gairdneri</name>
        <dbReference type="NCBI Taxonomy" id="8022"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo salar</name>
        <name type="common">Atlantic salmon</name>
        <dbReference type="NCBI Taxonomy" id="8030"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo trutta</name>
        <name type="common">Brown trout</name>
        <dbReference type="NCBI Taxonomy" id="8032"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pollachius virens</name>
        <name type="common">Saithe</name>
        <name type="synonym">Gadus virens</name>
        <dbReference type="NCBI Taxonomy" id="8060"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Gadus morhua</name>
        <name type="common">Atlantic cod</name>
        <dbReference type="NCBI Taxonomy" id="8049"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="J. Gen. Virol." volume="83" first="421" last="428">
            <title>Genomic organization of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="Clouthier S.C."/>
                <person name="Rector T."/>
                <person name="Brown N.E."/>
                <person name="Anderson E.D."/>
            </authorList>
            <dbReference type="PubMed" id="11807235"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Virus Res." volume="115" first="176" last="184">
            <title>Identification of an interferon antagonist protein encoded by segment 7 of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="McBeath A.J."/>
                <person name="Collet B."/>
                <person name="Paley R."/>
                <person name="Duraffour S."/>
                <person name="Aspehaug V."/>
                <person name="Biering E."/>
                <person name="Secombes C.J."/>
                <person name="Snow M."/>
            </authorList>
            <dbReference type="PubMed" id="16202469"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2005.08.005"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="Virus Res." volume="133" first="228" last="238">
            <title>Molecular and functional characterization of two infectious salmon anaemia virus (ISAV) proteins with type I interferon antagonizing activity.</title>
            <authorList>
                <person name="Garcia-Rosado E."/>
                <person name="Markussen T."/>
                <person name="Kileng O."/>
                <person name="Baekkevold E.S."/>
                <person name="Robertsen B."/>
                <person name="Mjaaland S."/>
                <person name="Rimstad E."/>
            </authorList>
            <dbReference type="PubMed" id="18304672"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2008.01.008"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2011" name="Virus Res." volume="155" first="10" last="19">
            <title>Infectious salmon anemia virus--genetics and pathogenesis.</title>
            <authorList>
                <person name="Cottet L."/>
                <person name="Rivas-Aravena A."/>
                <person name="Cortez-San Martin M."/>
                <person name="Sandino A.M."/>
                <person name="Spencer E."/>
            </authorList>
            <dbReference type="PubMed" id="20979983"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.10.021"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2013" name="Virus Res." volume="177" first="1" last="10">
            <title>Infectious salmon anaemia virus nuclear export protein is encoded by a spliced gene product of genomic segment 7.</title>
            <authorList>
                <person name="Ramly R.B."/>
                <person name="Olsen C.M."/>
                <person name="Braaen S."/>
                <person name="Rimstad E."/>
            </authorList>
            <dbReference type="PubMed" id="23850870"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2013.07.001"/>
        </citation>
        <scope>ALTERNATIVE SPLICING</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Plays a role in inhibition of the host innate immune system by counteracting the type I interferon signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host cytoplasm</location>
            <location evidence="2">Host perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 2">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="alternative splicing"/>
        <isoform>
            <id>Q8V3U2-1</id>
            <name>Non-structural protein 1</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q8V3U1-1</id>
            <name evidence="3">Nuclear export protein</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <dbReference type="EMBL" id="AF404341">
        <property type="protein sequence ID" value="AAL67956.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF429989">
        <property type="protein sequence ID" value="AAN57723.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145798.1">
        <property type="nucleotide sequence ID" value="NC_006498.1"/>
    </dbReference>
    <dbReference type="GeneID" id="5075850"/>
    <dbReference type="KEGG" id="vg:5075850"/>
    <dbReference type="Proteomes" id="UP000008772">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0025">Alternative splicing</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1114">Inhibition of host interferon signaling pathway by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="chain" id="PRO_0000403922" description="Non-structural protein 1">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="16202469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18304672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="23850870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="20979983"/>
        </source>
    </evidence>
    <sequence length="256" mass="28604" checksum="FEE8282488010E94" modified="2002-03-01" version="1">MSGFNFEVMVPEQGGKVVFSLTETGSCVSFYGDDEPGEGSCELASENMDFPSCPLGNGDDFCLSLALSTMRWSGMTKRNNFMDRFIGSFVHCTPVMIWSYGNLSKKSHHKMVCHTCPDEYKFSDKDEMQGYYEECLEASTDIFLDELATVVTGGFFPVGLKGSWGGWYLKYVRYAGPLAGSSGFIVNQRFYDRAQNKTGSRVVSMVEMDGDGLSFIYEKPSVYHSDGCTGSAARFWKRDHNERAGVELRAGLHFRM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>Q8V3U3</accession>
    <name>P7_ISAV8</name>
    <protein>
        <recommendedName>
            <fullName>Protein P7</fullName>
            <shortName>P7</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Segment-8</name>
        <name type="ORF">s8ORF2</name>
    </gene>
    <organism>
        <name type="scientific">Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)</name>
        <name type="common">ISAV</name>
        <dbReference type="NCBI Taxonomy" id="652965"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Insthoviricetes</taxon>
            <taxon>Articulavirales</taxon>
            <taxon>Orthomyxoviridae</taxon>
            <taxon>Isavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oncorhynchus kisutch</name>
        <name type="common">Coho salmon</name>
        <name type="synonym">Salmo kisutch</name>
        <dbReference type="NCBI Taxonomy" id="8019"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Oncorhynchus mykiss</name>
        <name type="common">Rainbow trout</name>
        <name type="synonym">Salmo gairdneri</name>
        <dbReference type="NCBI Taxonomy" id="8022"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo salar</name>
        <name type="common">Atlantic salmon</name>
        <dbReference type="NCBI Taxonomy" id="8030"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo trutta</name>
        <name type="common">Brown trout</name>
        <dbReference type="NCBI Taxonomy" id="8032"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pollachius virens</name>
        <name type="common">Saithe</name>
        <name type="synonym">Gadus virens</name>
        <dbReference type="NCBI Taxonomy" id="8060"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Gadus morhua</name>
        <name type="common">Atlantic cod</name>
        <dbReference type="NCBI Taxonomy" id="8049"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="J. Gen. Virol." volume="83" first="421" last="428">
            <title>Genomic organization of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="Clouthier S.C."/>
                <person name="Rector T."/>
                <person name="Brown N.E."/>
                <person name="Anderson E.D."/>
            </authorList>
            <dbReference type="PubMed" id="11807235"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Virus Res." volume="155" first="10" last="19">
            <title>Infectious salmon anemia virus--genetics and pathogenesis.</title>
            <authorList>
                <person name="Cottet L."/>
                <person name="Rivas-Aravena A."/>
                <person name="Cortez-San Martin M."/>
                <person name="Sandino A.M."/>
                <person name="Spencer E."/>
            </authorList>
            <dbReference type="PubMed" id="20979983"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.10.021"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2016" name="Fish Shellfish Immunol." volume="52" first="258" last="262">
            <title>Infectious salmon anemia virus segment 7 ORF1 and segment 8 ORF2 proteins inhibit IRF mediated activation of the Atlantic salmon IFNa1 promoter.</title>
            <authorList>
                <person name="Li C."/>
                <person name="Greiner-Tollersrud L."/>
                <person name="Robertsen B."/>
            </authorList>
            <dbReference type="PubMed" id="27012395"/>
            <dbReference type="DOI" id="10.1016/j.fsi.2016.03.038"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in inhibition of the host immune system by counteracting the type I interferon response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="alternative splicing"/>
        <isoform>
            <id>Q8V3U3-1</id>
            <name>Matrix protein 2</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q8V3U4-1</id>
            <name>Matrix protein 1</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <dbReference type="EMBL" id="AF404340">
        <property type="protein sequence ID" value="AAL67955.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145797.1">
        <property type="nucleotide sequence ID" value="NC_006497.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q8V3U3"/>
    <dbReference type="GeneID" id="5075848"/>
    <dbReference type="KEGG" id="vg:5075848"/>
    <dbReference type="Proteomes" id="UP000008772">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0025">Alternative splicing</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="chain" id="PRO_0000403925" description="Protein P7">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="27012395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="20979983"/>
        </source>
    </evidence>
    <sequence length="183" mass="20340" checksum="7FE092EDC900FA6B" modified="2002-03-01" version="1">MREANPKPRELIRHALKKKKRPEVVYAMGVLLTLGGESGLTVEFPVPEGKTVKVKTLNQLVNGMISRATMTLYCVMKDPPSGGMATLMRDHIRNWLKEESGCQDADGGEEKWAMVYGMISPDMAEEKTMLKELKTMLHSRMQMYALGASSKALENLEKAIVAAVHRLPASCSTEKMVLLGYLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8V3U4</accession>
    <name>M1_ISAV8</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Matrix protein 1</fullName>
            <shortName>M1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P6</fullName>
            <shortName>P6</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>vp22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Segment-8</name>
        <name type="ORF">s8ORF1</name>
    </gene>
    <organism>
        <name type="scientific">Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)</name>
        <name type="common">ISAV</name>
        <dbReference type="NCBI Taxonomy" id="652965"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Insthoviricetes</taxon>
            <taxon>Articulavirales</taxon>
            <taxon>Orthomyxoviridae</taxon>
            <taxon>Isavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oncorhynchus kisutch</name>
        <name type="common">Coho salmon</name>
        <name type="synonym">Salmo kisutch</name>
        <dbReference type="NCBI Taxonomy" id="8019"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Oncorhynchus mykiss</name>
        <name type="common">Rainbow trout</name>
        <name type="synonym">Salmo gairdneri</name>
        <dbReference type="NCBI Taxonomy" id="8022"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo salar</name>
        <name type="common">Atlantic salmon</name>
        <dbReference type="NCBI Taxonomy" id="8030"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmo trutta</name>
        <name type="common">Brown trout</name>
        <dbReference type="NCBI Taxonomy" id="8032"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pollachius virens</name>
        <name type="common">Saithe</name>
        <name type="synonym">Gadus virens</name>
        <dbReference type="NCBI Taxonomy" id="8060"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Gadus morhua</name>
        <name type="common">Atlantic cod</name>
        <dbReference type="NCBI Taxonomy" id="8049"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="J. Gen. Virol." volume="83" first="421" last="428">
            <title>Genomic organization of infectious salmon anaemia virus.</title>
            <authorList>
                <person name="Clouthier S.C."/>
                <person name="Rector T."/>
                <person name="Brown N.E."/>
                <person name="Anderson E.D."/>
            </authorList>
            <dbReference type="PubMed" id="11807235"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Virol." volume="78" first="3063" last="3071">
            <title>Identification and characterization of viral structural proteins of infectious salmon anemia virus.</title>
            <authorList>
                <person name="Falk K."/>
                <person name="Aspehaug V."/>
                <person name="Vlasak R."/>
                <person name="Endresen C."/>
            </authorList>
            <dbReference type="PubMed" id="14990725"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="Virus Res." volume="155" first="10" last="19">
            <title>Infectious salmon anemia virus--genetics and pathogenesis.</title>
            <authorList>
                <person name="Cottet L."/>
                <person name="Rivas-Aravena A."/>
                <person name="Cortez-San Martin M."/>
                <person name="Sandino A.M."/>
                <person name="Spencer E."/>
            </authorList>
            <dbReference type="PubMed" id="20979983"/>
            <dbReference type="DOI" id="10.1016/j.virusres.2010.10.021"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2017" name="Virol. J." volume="14" first="30" last="30">
            <title>Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70.</title>
            <authorList>
                <person name="Zhang W."/>
                <person name="Cai C."/>
                <person name="Lin L."/>
                <person name="Tao Y.J."/>
                <person name="Jin M."/>
            </authorList>
            <dbReference type="PubMed" id="28202040"/>
            <dbReference type="DOI" id="10.1186/s12985-017-0702-z"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INTERACTION WITH HOST HSC70</scope>
    </reference>
    <comment type="function">
        <text evidence="6 7">May play a role in virus replication, from virus entry and uncoating to assembly and budding of the virus particle (PubMed:20979983). Interaction of viral NEP with M1-Hsc70 is thought to promote nuclear export of the viral encapsidated genomes (PubMed:28202040).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with host HSC70.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="alternative splicing"/>
        <isoform>
            <id>Q8V3U4-1</id>
            <name>Matrix protein 1</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q8V3U3-1</id>
            <name>Matrix protein 2</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <dbReference type="EMBL" id="AF404340">
        <property type="protein sequence ID" value="AAL67954.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145796.1">
        <property type="nucleotide sequence ID" value="NC_006497.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q8V3U4"/>
    <dbReference type="GeneID" id="5075847"/>
    <dbReference type="KEGG" id="vg:5075847"/>
    <dbReference type="Proteomes" id="UP000008772">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0025">Alternative splicing</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000403924" description="Matrix protein 1">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="14990725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="28202040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="14990725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="20979983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="7">
        <source>
            <dbReference type="PubMed" id="28202040"/>
        </source>
    </evidence>
    <sequence length="234" mass="26539" checksum="51072C3F92545CB0" modified="2002-03-01" version="1">MHERSKPKTTGADQTCLEEEKETRGGLRNGSSSDTGGRERTDRGVSCSRRKNCEGQNLEPIGERDDQSSDDDPLLCDERSTIGRHGNADERPHQELAEGGIRMPGRGWWRGKMGNGVWYDFTRHGRGEDDAEGAENNATQQDADVCSGCKFESPREFRKGHRRCSSSTSGILLDREDGASGVPEVSFKERMEAEKKKLKELDDKIYKLRRRLRKMEYKKMGINREIDKLEDSVQ</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>