<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>P0C530</accession>
    <name>NS3B_BC133</name>
    <protein>
        <recommendedName>
            <fullName>Non-structural protein 3b</fullName>
            <shortName>ns3b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Accessory protein 3b</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">3b</name>
    </gene>
    <organism>
        <name type="scientific">Bat coronavirus 133/2005</name>
        <name type="common">BtCoV</name>
        <name type="synonym">BtCoV/133/2005</name>
        <dbReference type="NCBI Taxonomy" id="389230"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Nidovirales</taxon>
            <taxon>Cornidovirineae</taxon>
            <taxon>Coronaviridae</taxon>
            <taxon>Orthocoronavirinae</taxon>
            <taxon>Betacoronavirus</taxon>
            <taxon>Merbecovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Tylonycteris pachypus</name>
        <name type="common">Lesser bamboo bat</name>
        <name type="synonym">Vespertilio pachypus</name>
        <dbReference type="NCBI Taxonomy" id="258959"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="7481" last="7490">
            <title>Prevalence and genetic diversity of coronaviruses in bats from China.</title>
            <authorList>
                <person name="Tang X.C."/>
                <person name="Zhang J.X."/>
                <person name="Zhang S.Y."/>
                <person name="Wang P."/>
                <person name="Fan X.H."/>
                <person name="Li L.F."/>
                <person name="Li G."/>
                <person name="Dong B.Q."/>
                <person name="Liu W."/>
                <person name="Cheung C.L."/>
                <person name="Xu K.M."/>
                <person name="Song W.J."/>
                <person name="Vijaykrishna D."/>
                <person name="Poon L.L.M."/>
                <person name="Peiris J.S.M."/>
                <person name="Smith G.J."/>
                <person name="Chen H."/>
                <person name="Guan Y."/>
            </authorList>
            <dbReference type="PubMed" id="16840328"/>
            <dbReference type="DOI" id="10.1128/JVI.00697-06"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Induces host cell G0/G1 arrest and apoptosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host RUNX1 isoform b.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
            <location evidence="1">Host nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DQ648794">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="SMR" id="P0C530"/>
    <dbReference type="Proteomes" id="UP000007449">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033650">
        <property type="term" value="C:host cell mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044196">
        <property type="term" value="C:host cell nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039526">
        <property type="term" value="P:modulation by virus of host apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039646">
        <property type="term" value="P:modulation by virus of host G0/G1 transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0053">Apoptosis</keyword>
    <keyword id="KW-1077">G0/G1 host cell cycle checkpoint dysregulation by virus</keyword>
    <keyword id="KW-1045">Host mitochondrion</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1119">Modulation of host cell apoptosis by virus</keyword>
    <keyword id="KW-1121">Modulation of host cell cycle by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000290264" description="Non-structural protein 3b">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P59633"/>
        </source>
    </evidence>
    <sequence length="119" mass="13898" checksum="CD1E1E9500F17976" modified="2007-06-12" version="1">MDYVSLLNQFWQKQIKSYKETPSQYHYLYPPRFFYKPVLGNLQHPTKWCCTIKFYEYSAQATECTKASAKQDAARLICEQLQAAGLLNGMELRFRSSASDIFGQNRYDASKSYFFSKTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-06-10" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>P0C6F7</accession>
    <accession>Q0Q4F3</accession>
    <name>R1A_BC133</name>
    <protein>
        <recommendedName>
            <fullName>Replicase polyprotein 1a</fullName>
            <shortName>pp1a</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>ORF1a polyprotein</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 1</fullName>
                <shortName>nsp1</shortName>
            </recommendedName>
            <alternativeName>
                <fullName>Leader protein</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 2</fullName>
                <shortName>nsp2</shortName>
            </recommendedName>
            <alternativeName>
                <fullName>p65 homolog</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 3</fullName>
                <shortName>nsp3</shortName>
                <ecNumber>3.4.19.12</ecNumber>
                <ecNumber>3.4.22.69</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>PL2-PRO</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>Papain-like proteinase</fullName>
                <shortName>PL-PRO</shortName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 4</fullName>
                <shortName>nsp4</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>3C-like proteinase</fullName>
                <shortName>3CL-PRO</shortName>
                <shortName>3CLp</shortName>
                <ecNumber>3.4.22.-</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>nsp5</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 6</fullName>
                <shortName>nsp6</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 7</fullName>
                <shortName>nsp7</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 8</fullName>
                <shortName>nsp8</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 9</fullName>
                <shortName>nsp9</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 10</fullName>
                <shortName>nsp10</shortName>
            </recommendedName>
            <alternativeName>
                <fullName>Growth factor-like peptide</fullName>
                <shortName>GFL</shortName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 11</fullName>
                <shortName>nsp11</shortName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="ORF">1a</name>
    </gene>
    <organism>
        <name type="scientific">Bat coronavirus 133/2005</name>
        <name type="common">BtCoV</name>
        <name type="synonym">BtCoV/133/2005</name>
        <dbReference type="NCBI Taxonomy" id="389230"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Nidovirales</taxon>
            <taxon>Cornidovirineae</taxon>
            <taxon>Coronaviridae</taxon>
            <taxon>Orthocoronavirinae</taxon>
            <taxon>Betacoronavirus</taxon>
            <taxon>Merbecovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Tylonycteris pachypus</name>
        <name type="common">Lesser bamboo bat</name>
        <name type="synonym">Vespertilio pachypus</name>
        <dbReference type="NCBI Taxonomy" id="258959"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="7481" last="7490">
            <title>Prevalence and genetic diversity of coronaviruses in bats from China.</title>
            <authorList>
                <person name="Tang X.C."/>
                <person name="Zhang J.X."/>
                <person name="Zhang S.Y."/>
                <person name="Wang P."/>
                <person name="Fan X.H."/>
                <person name="Li L.F."/>
                <person name="Li G."/>
                <person name="Dong B.Q."/>
                <person name="Liu W."/>
                <person name="Cheung C.L."/>
                <person name="Xu K.M."/>
                <person name="Song W.J."/>
                <person name="Vijaykrishna D."/>
                <person name="Poon L.L.M."/>
                <person name="Peiris J.S.M."/>
                <person name="Smith G.J."/>
                <person name="Chen H."/>
                <person name="Guan Y."/>
            </authorList>
            <dbReference type="PubMed" id="16840328"/>
            <dbReference type="DOI" id="10.1128/JVI.00697-06"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="J. Virol." volume="83" first="5282" last="5288">
            <title>Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses.</title>
            <authorList>
                <person name="Tohya Y."/>
                <person name="Narayanan K."/>
                <person name="Kamitani W."/>
                <person name="Huang C."/>
                <person name="Lokugamage K."/>
                <person name="Makino S."/>
            </authorList>
            <dbReference type="PubMed" id="19264783"/>
            <dbReference type="DOI" id="10.1128/JVI.02485-08"/>
        </citation>
        <scope>FUNCTION OF NSP1</scope>
    </reference>
    <comment type="function">
        <text evidence="22">The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.</text>
    </comment>
    <comment type="function">
        <molecule>3C-like proteinase</molecule>
        <text evidence="6">Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Nsp9 is a ssRNA-binding protein.</text>
    </comment>
    <comment type="function">
        <molecule>Non-structural protein 1</molecule>
        <text evidence="22">Binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.</text>
            <dbReference type="EC" id="3.4.22.69"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 3</molecule>
        <subcellularLocation>
            <location evidence="23">Host membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 4</molecule>
        <subcellularLocation>
            <location evidence="23">Host membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 6</molecule>
        <subcellularLocation>
            <location evidence="23">Host membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 7</molecule>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
            <location evidence="1">Host perinuclear region</location>
        </subcellularLocation>
        <text evidence="1">nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 8</molecule>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
            <location evidence="1">Host perinuclear region</location>
        </subcellularLocation>
        <text evidence="1">nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 9</molecule>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
            <location evidence="1">Host perinuclear region</location>
        </subcellularLocation>
        <text evidence="1">nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 10</molecule>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
            <location evidence="1">Host perinuclear region</location>
        </subcellularLocation>
        <text evidence="1">nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).</text>
    </comment>
    <comment type="alternative products">
        <event type="ribosomal frameshifting"/>
        <isoform>
            <id>P0C6F7-1</id>
            <name>Replicase polyprotein 1a</name>
            <name>pp1a</name>
            <name>ORF1a polyprotein</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>P0C6W1-1</id>
            <name>Replicase polyprotein 1ab</name>
            <name>pp1ab</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <comment type="domain">
        <text evidence="1">The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed.</text>
    </comment>
    <comment type="miscellaneous">
        <molecule>Isoform Replicase polyprotein 1a</molecule>
        <text>Produced by conventional translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the coronaviruses polyprotein 1ab family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12"/>
    <dbReference type="EC" id="3.4.22.69"/>
    <dbReference type="EC" id="3.4.22.-"/>
    <dbReference type="EMBL" id="DQ648794">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="SMR" id="P0C6F7"/>
    <dbReference type="Proteomes" id="UP000007449">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033644">
        <property type="term" value="C:host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044220">
        <property type="term" value="C:host cell perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002151">
        <property type="term" value="F:G-quadruplex RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039595">
        <property type="term" value="P:induction by virus of catabolism of host mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039520">
        <property type="term" value="P:induction by virus of host autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039648">
        <property type="term" value="P:modulation by virus of host protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039657">
        <property type="term" value="P:suppression by virus of host gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039579">
        <property type="term" value="P:suppression by virus of host ISG15-protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039502">
        <property type="term" value="P:suppression by virus of host type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039548">
        <property type="term" value="P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019079">
        <property type="term" value="P:viral genome replication"/>
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            <begin position="1329"/>
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    </feature>
    <feature type="domain" description="DPUP" evidence="7">
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            <begin position="1453"/>
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    <feature type="domain" description="Ubiquitin-like 2" evidence="3">
        <location>
            <begin position="1531"/>
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    </feature>
    <feature type="domain" description="Peptidase C16" evidence="4">
        <location>
            <begin position="1600"/>
            <end position="1871"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleic acid-binding" evidence="8">
        <location>
            <begin position="1885"/>
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    </feature>
    <feature type="domain" description="G2M" evidence="20">
        <location>
            <begin position="2019"/>
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    </feature>
    <feature type="domain" description="CoV Nsp3 Y3" evidence="19">
        <location>
            <begin position="2686"/>
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        </location>
    </feature>
    <feature type="domain" description="Nsp4C" evidence="9">
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            <begin position="3202"/>
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    <feature type="domain" description="Peptidase C30" evidence="6">
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            <begin position="3299"/>
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    <feature type="domain" description="RdRp Nsp7 cofactor" evidence="10">
        <location>
            <begin position="3897"/>
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            <begin position="3980"/>
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    <feature type="domain" description="Nsp9 ssRNA-binding" evidence="12">
        <location>
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    <feature type="domain" description="ExoN/MTase coactivator" evidence="13">
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            <begin position="4289"/>
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            <begin position="1721"/>
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    <feature type="zinc finger region" evidence="1">
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    <feature type="zinc finger region" evidence="1">
        <location>
            <begin position="4404"/>
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    <feature type="region of interest" description="C4" evidence="16">
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            <begin position="339"/>
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            <begin position="1040"/>
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            <name>Zn(2+)</name>
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            <name>Zn(2+)</name>
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            <name>Zn(2+)</name>
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            <name>Zn(2+)</name>
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        <ligand>
            <name>Zn(2+)</name>
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        <location>
            <position position="4415"/>
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        <ligand>
            <name>Zn(2+)</name>
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        <location>
            <position position="4417"/>
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        <ligand>
            <name>Zn(2+)</name>
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    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
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            <dbReference type="PROSITE-ProRule" id="PRU00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00444"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00490"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00772"/>
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    </evidence>
    <evidence type="ECO:0000255" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01289"/>
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    </evidence>
    <evidence type="ECO:0000255" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01290"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01291"/>
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    <evidence type="ECO:0000255" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01294"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01296"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01297"/>
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    </evidence>
    <evidence type="ECO:0000255" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01307"/>
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    </evidence>
    <evidence type="ECO:0000255" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01308"/>
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    <evidence type="ECO:0000255" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="22">
        <source>
            <dbReference type="PubMed" id="19264783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="23"/>
    <sequence length="4441" mass="492464" checksum="A6E0DB4CDDA40AC7" modified="2008-06-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-06-10" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>P0C6W1</accession>
    <accession>Q0Q4F3</accession>
    <name>R1AB_BC133</name>
    <protein>
        <recommendedName>
            <fullName>Replicase polyprotein 1ab</fullName>
            <shortName>pp1ab</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>ORF1ab polyprotein</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Host translation inhibitor nsp1</fullName>
                <shortName>nsp1</shortName>
            </recommendedName>
            <alternativeName>
                <fullName>Leader protein</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 2</fullName>
                <shortName>nsp2</shortName>
            </recommendedName>
            <alternativeName>
                <fullName>p65 homolog</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Papain-like proteinase</fullName>
                <shortName>PL-PRO</shortName>
                <ecNumber>3.4.19.12</ecNumber>
                <ecNumber>3.4.22.-</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Non-structural protein 3</fullName>
                <shortName>nsp3</shortName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 4</fullName>
                <shortName>nsp4</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>3C-like proteinase</fullName>
                <shortName>3CL-PRO</shortName>
                <shortName>3CLp</shortName>
                <ecNumber>3.4.22.-</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>nsp5</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 6</fullName>
                <shortName>nsp6</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 7</fullName>
                <shortName>nsp7</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 8</fullName>
                <shortName>nsp8</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 9</fullName>
                <shortName>nsp9</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Non-structural protein 10</fullName>
                <shortName>nsp10</shortName>
            </recommendedName>
            <alternativeName>
                <fullName>Growth factor-like peptide</fullName>
                <shortName>GFL</shortName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>RNA-directed RNA polymerase</fullName>
                <shortName>Pol</shortName>
                <shortName>RdRp</shortName>
                <ecNumber>2.7.7.48</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>nsp12</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Helicase</fullName>
                <shortName>Hel</shortName>
                <ecNumber>3.6.4.12</ecNumber>
                <ecNumber>3.6.4.13</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>nsp13</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Guanine-N7 methyltransferase</fullName>
                <shortName>ExoN</shortName>
                <ecNumber>2.1.1.-</ecNumber>
                <ecNumber>3.1.13.-</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>nsp14</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Uridylate-specific endoribonuclease</fullName>
                <ecNumber>4.6.1.-</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>NendoU</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>nsp15</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>2'-O-methyltransferase</fullName>
                <ecNumber>2.1.1.57</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>nsp16</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name type="primary">rep</name>
        <name type="ORF">1a-1b</name>
    </gene>
    <organism>
        <name type="scientific">Bat coronavirus 133/2005</name>
        <name type="common">BtCoV</name>
        <name type="synonym">BtCoV/133/2005</name>
        <dbReference type="NCBI Taxonomy" id="389230"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Nidovirales</taxon>
            <taxon>Cornidovirineae</taxon>
            <taxon>Coronaviridae</taxon>
            <taxon>Orthocoronavirinae</taxon>
            <taxon>Betacoronavirus</taxon>
            <taxon>Merbecovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Tylonycteris pachypus</name>
        <name type="common">Lesser bamboo bat</name>
        <name type="synonym">Vespertilio pachypus</name>
        <dbReference type="NCBI Taxonomy" id="258959"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="7481" last="7490">
            <title>Prevalence and genetic diversity of coronaviruses in bats from China.</title>
            <authorList>
                <person name="Tang X.C."/>
                <person name="Zhang J.X."/>
                <person name="Zhang S.Y."/>
                <person name="Wang P."/>
                <person name="Fan X.H."/>
                <person name="Li L.F."/>
                <person name="Li G."/>
                <person name="Dong B.Q."/>
                <person name="Liu W."/>
                <person name="Cheung C.L."/>
                <person name="Xu K.M."/>
                <person name="Song W.J."/>
                <person name="Vijaykrishna D."/>
                <person name="Poon L.L.M."/>
                <person name="Peiris J.S.M."/>
                <person name="Smith G.J."/>
                <person name="Chen H."/>
                <person name="Guan Y."/>
            </authorList>
            <dbReference type="PubMed" id="16840328"/>
            <dbReference type="DOI" id="10.1128/JVI.00697-06"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="J. Virol." volume="83" first="5282" last="5288">
            <title>Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses.</title>
            <authorList>
                <person name="Tohya Y."/>
                <person name="Narayanan K."/>
                <person name="Kamitani W."/>
                <person name="Huang C."/>
                <person name="Lokugamage K."/>
                <person name="Makino S."/>
            </authorList>
            <dbReference type="PubMed" id="19264783"/>
            <dbReference type="DOI" id="10.1128/JVI.02485-08"/>
        </citation>
        <scope>FUNCTION OF NSP1</scope>
    </reference>
    <comment type="function">
        <text evidence="2">The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.</text>
    </comment>
    <comment type="function">
        <molecule>Host translation inhibitor nsp1</molecule>
        <text evidence="2 33">Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.</text>
    </comment>
    <comment type="function">
        <molecule>Non-structural protein 2</molecule>
        <text evidence="2">May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.</text>
    </comment>
    <comment type="function">
        <molecule>Papain-like proteinase</molecule>
        <text evidence="2">Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.</text>
    </comment>
    <comment type="function">
        <molecule>Non-structural protein 4</molecule>
        <text evidence="2">Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.</text>
    </comment>
    <comment type="function">
        <molecule>3C-like proteinase</molecule>
        <text evidence="2 8">Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).</text>
    </comment>
    <comment type="function">
        <molecule>Non-structural protein 6</molecule>
        <text evidence="2">Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.</text>
    </comment>
    <comment type="function">
        <molecule>Non-structural protein 7</molecule>
        <text evidence="2">Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.</text>
    </comment>
    <comment type="function">
        <molecule>Non-structural protein 8</molecule>
        <text evidence="2">Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.</text>
    </comment>
    <comment type="function">
        <molecule>Non-structural protein 9</molecule>
        <text evidence="2">May participate in viral replication by acting as a ssRNA-binding protein.</text>
    </comment>
    <comment type="function">
        <molecule>Non-structural protein 10</molecule>
        <text evidence="2">Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.</text>
    </comment>
    <comment type="function">
        <molecule>RNA-directed RNA polymerase</molecule>
        <text evidence="2">Responsible for replication and transcription of the viral RNA genome.</text>
    </comment>
    <comment type="function">
        <molecule>Helicase</molecule>
        <text evidence="2">Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.</text>
    </comment>
    <comment type="function">
        <molecule>Guanine-N7 methyltransferase</molecule>
        <text evidence="2">Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.</text>
    </comment>
    <comment type="function">
        <molecule>Uridylate-specific endoribonuclease</molecule>
        <text evidence="2">Plays a role in viral transcription/replication and prevents the simultaneous activation of host cell dsRNA sensors, such as MDA5/IFIH1, OAS, and PKR (By similarity). Acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P) (By similarity). If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails and activate host dsRNA sensors.</text>
    </comment>
    <comment type="function">
        <molecule>2'-O-methyltransferase</molecule>
        <text evidence="2">Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.</text>
    </comment>
    <comment type="catalytic activity">
        <molecule>Helicase</molecule>
        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <molecule>Helicase</molecule>
        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <molecule>RNA-directed RNA polymerase</molecule>
        <reaction evidence="7">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <molecule>Papain-like proteinase</molecule>
        <reaction>
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <molecule>2'-O-methyltransferase</molecule>
        <reaction evidence="2">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:67020"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="Rhea" id="RHEA-COMP:17168"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167609"/>
            <dbReference type="EC" id="2.1.1.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <molecule>Uridylate-specific endoribonuclease</molecule>
        <reaction evidence="2">
            <text>uridylyl-uridylyl-ribonucleotide-RNA = a 3'-end uridylyl-2',3'-cyclophospho-uridine-RNA + a 5'-end dephospho-ribonucleoside-RNA</text>
            <dbReference type="Rhea" id="RHEA:67732"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17334"/>
            <dbReference type="Rhea" id="RHEA-COMP:17335"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173079"/>
            <dbReference type="ChEBI" id="CHEBI:173080"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <molecule>Uridylate-specific endoribonuclease</molecule>
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">Likely affects Nsp15 binding to RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Nsp2 interacts with host PHB and PHB2. 3CL-PRO exists as monomer and homodimer. Nsp4 interacts with PL-PRO and nsp6. Only the homodimer shows catalytic activity. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure. Nsp9 is a dimer. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 enzymatic activities. Nsp14 interacts (via N-terminus) with DDX1.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Papain-like proteinase</molecule>
        <subcellularLocation>
            <location>Host membrane</location>
            <topology>Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 4</molecule>
        <subcellularLocation>
            <location>Host membrane</location>
            <topology>Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location>Host cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Localizes in virally-induced cytoplasmic double-membrane vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 6</molecule>
        <subcellularLocation>
            <location evidence="34">Host membrane</location>
            <topology evidence="34">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 7</molecule>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
            <location evidence="1">Host perinuclear region</location>
        </subcellularLocation>
        <text evidence="1">nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 8</molecule>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
            <location evidence="1">Host perinuclear region</location>
        </subcellularLocation>
        <text evidence="1">nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 9</molecule>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
            <location evidence="1">Host perinuclear region</location>
        </subcellularLocation>
        <text evidence="1">nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Non-structural protein 10</molecule>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
            <location evidence="1">Host perinuclear region</location>
        </subcellularLocation>
        <text evidence="1">nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Helicase</molecule>
        <subcellularLocation>
            <location evidence="34">Host endoplasmic reticulum-Golgi intermediate compartment</location>
        </subcellularLocation>
        <text evidence="1">The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Uridylate-specific endoribonuclease</molecule>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
            <location evidence="1">Host perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="ribosomal frameshifting"/>
        <isoform>
            <id>P0C6W1-1</id>
            <name>Replicase polyprotein 1ab</name>
            <name>pp1ab</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>P0C6F7-1</id>
            <name>Replicase polyprotein 1a</name>
            <name>pp1a</name>
            <name>ORF1a polyprotein</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <comment type="domain">
        <text evidence="1">The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed.</text>
    </comment>
    <comment type="miscellaneous">
        <molecule>Isoform Replicase polyprotein 1ab</molecule>
        <text>Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.</text>
    </comment>
    <comment type="similarity">
        <text evidence="34">Belongs to the coronaviruses polyprotein 1ab family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12"/>
    <dbReference type="EC" id="3.4.22.-"/>
    <dbReference type="EC" id="2.7.7.48"/>
    <dbReference type="EC" id="3.6.4.12"/>
    <dbReference type="EC" id="3.6.4.13"/>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EC" id="3.1.13.-"/>
    <dbReference type="EC" id="4.6.1.-"/>
    <dbReference type="EC" id="2.1.1.57"/>
    <dbReference type="EMBL" id="DQ648794">
        <property type="protein sequence ID" value="ABG47051.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="SMR" id="P0C6W1"/>
    <dbReference type="Proteomes" id="UP000007449">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044172">
        <property type="term" value="C:host cell endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033644">
        <property type="term" value="C:host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044220">
        <property type="term" value="C:host cell perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004532">
        <property type="term" value="F:exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002151">
        <property type="term" value="F:G-quadruplex RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039595">
        <property type="term" value="P:induction by virus of catabolism of host mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039520">
        <property type="term" value="P:induction by virus of host autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039648">
        <property type="term" value="P:modulation by virus of host protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039657">
        <property type="term" value="P:suppression by virus of host gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039579">
        <property type="term" value="P:suppression by virus of host ISG15-protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039644">
        <property type="term" value="P:suppression by virus of host NF-kappaB cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039502">
        <property type="term" value="P:suppression by virus of host type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019082">
        <property type="term" value="P:viral protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039694">
        <property type="term" value="P:viral RNA genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21409">
        <property type="entry name" value="1B_cv_Nsp13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21560">
        <property type="entry name" value="betaCoV-Nsp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21659">
        <property type="entry name" value="betaCoV_Nsp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21666">
        <property type="entry name" value="betaCoV_Nsp5_Mpro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20762">
        <property type="entry name" value="capping_2-OMTase_Nidovirales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21473">
        <property type="entry name" value="cv_Nsp4_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21167">
        <property type="entry name" value="M_alpha_beta_cv_Nsp15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21563">
        <property type="entry name" value="Macro_cv_SUD-M_Nsp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21557">
        <property type="entry name" value="Macro_X_Nsp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21592">
        <property type="entry name" value="MERS-CoV-like_RdRp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21161">
        <property type="entry name" value="NendoU_cv_Nsp15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21171">
        <property type="entry name" value="NTD_alpha_beta_cv_Nsp15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21523">
        <property type="entry name" value="SUD_C_MERS-CoV_Nsp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21467">
        <property type="entry name" value="Ubl1_cv_Nsp3_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21466">
        <property type="entry name" value="Ubl2_cv_PLpro_N_Nsp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21401">
        <property type="entry name" value="ZBD_cv_Nsp13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11580">
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    <keyword id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0693">Viral RNA replication</keyword>
    <keyword id="KW-0862">Zinc</keyword>
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        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="6406"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>8</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="6428"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>8</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="6439"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>8</label>
        </ligand>
    </feature>
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        <location>
            <position position="6442"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>8</label>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage" evidence="3">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by PL-PRO" evidence="3">
        <location>
            <begin position="847"/>
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    <evidence type="ECO:0000250" key="1"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PubMed" id="19264783"/>
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    <sequence length="7126" mass="795781" checksum="23A0EB77D0CFD96F" modified="2008-06-10" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q0Q4E6</accession>
    <name>NCAP_BC133</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoprotein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleocapsid protein</fullName>
            <shortName evidence="1">NC</shortName>
            <shortName evidence="1">Protein N</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">N</name>
        <name type="ORF">6</name>
    </gene>
    <organism>
        <name type="scientific">Bat coronavirus 133/2005</name>
        <name type="common">BtCoV</name>
        <name type="synonym">BtCoV/133/2005</name>
        <dbReference type="NCBI Taxonomy" id="389230"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Nidovirales</taxon>
            <taxon>Cornidovirineae</taxon>
            <taxon>Coronaviridae</taxon>
            <taxon>Orthocoronavirinae</taxon>
            <taxon>Betacoronavirus</taxon>
            <taxon>Merbecovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Tylonycteris pachypus</name>
        <name type="common">Lesser bamboo bat</name>
        <name type="synonym">Vespertilio pachypus</name>
        <dbReference type="NCBI Taxonomy" id="258959"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="7481" last="7490">
            <title>Prevalence and genetic diversity of coronaviruses in bats from China.</title>
            <authorList>
                <person name="Tang X.C."/>
                <person name="Zhang J.X."/>
                <person name="Zhang S.Y."/>
                <person name="Wang P."/>
                <person name="Fan X.H."/>
                <person name="Li L.F."/>
                <person name="Li G."/>
                <person name="Dong B.Q."/>
                <person name="Liu W."/>
                <person name="Cheung C.L."/>
                <person name="Xu K.M."/>
                <person name="Song W.J."/>
                <person name="Vijaykrishna D."/>
                <person name="Poon L.L.M."/>
                <person name="Peiris J.S.M."/>
                <person name="Smith G.J."/>
                <person name="Chen H."/>
                <person name="Guan Y."/>
            </authorList>
            <dbReference type="PubMed" id="16840328"/>
            <dbReference type="DOI" id="10.1128/JVI.00697-06"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with protein M. Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host endoplasmic reticulum-Golgi intermediate compartment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus</location>
        </subcellularLocation>
        <text evidence="1">Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated on serine and threonine residues.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the betacoronavirus nucleocapsid protein family.</text>
    </comment>
    <dbReference type="EMBL" id="DQ648794">
        <property type="protein sequence ID" value="ABG47058.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q0Q4E6"/>
    <dbReference type="Proteomes" id="UP000007449">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044172">
        <property type="term" value="C:host cell endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044177">
        <property type="term" value="C:host cell Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019013">
        <property type="term" value="C:viral nucleocapsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21595">
        <property type="entry name" value="CoV_N-CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CoV_N-NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BETA_CORONA_NCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044344">
        <property type="entry name" value="N_prot_C_CoV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044345">
        <property type="entry name" value="N_prot_N_CoV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043505">
        <property type="entry name" value="NCAP_bCoV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001218">
        <property type="entry name" value="Nucleocap_CoV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037179">
        <property type="entry name" value="Nucleocapsid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037195">
        <property type="entry name" value="Nucleocapsid_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00937">
        <property type="entry name" value="CoV_nucleocap"/>
        <property type="match status" value="1"/>
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    <dbReference type="PIRSF" id="PIRSF003888">
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    </dbReference>
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        <property type="entry name" value="COV_N_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0013">ADP-ribosylation</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0543">Viral nucleoprotein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000290258" description="Nucleoprotein">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="CoV N NTD" evidence="2">
        <location>
            <begin position="39"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="CoV N CTD" evidence="3">
        <location>
            <begin position="240"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA-binding" evidence="1">
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            <begin position="33"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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            <begin position="159"/>
            <end position="207"/>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Dimerization" evidence="1">
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            <begin position="251"/>
            <end position="360"/>
        </location>
    </feature>
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        <location>
            <begin position="365"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="163"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
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            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine; by host" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine; by host" evidence="1">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="424" mass="45952" checksum="4431AC2086660AAB" modified="2006-09-05" version="1">MATPAAPRTISFADNNDNQPNQQQRGRGRNPKPRPAPNNTVSWYTGLTQHGKNPLAFPPGQGVPLNANSTTAQNAGYWRRQDRKINTGNGVKQLAPRWFFYYTGTGPEANLPFRSVKDGIVWVYEEGATDAPSVFGTRNPANDAAIVCQFAPGTLIPKNFHIEGTGGNSQSSSRASSNSRNSSRSNSRGGRSTSNSRGTSPVSHGVGSAESLAALPLLLDLQKRLADLESGKSKQPKVVTKKDAAAAKNKMRHKRVATKGFNVTQAFGLRGPGPLQGNFGDMNYNKFGTEDPRWPQMAELAPSASAFMSMSQFKLTHQSNDDKGDPIYFLSYSGAIKLDPKNPNYKKWLELLEANIDAYKTFPKKERKPKTTEDGAVVASSSASQMEDVDAKPQRKPKSRVAGSITMRSGSSPALQDVTFDSEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q0Q4E7</accession>
    <name>VME1_BC133</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Membrane protein</fullName>
            <shortName evidence="1">M protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">E1 glycoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Matrix glycoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Membrane glycoprotein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">M</name>
        <name type="ORF">5</name>
    </gene>
    <organism>
        <name type="scientific">Bat coronavirus 133/2005</name>
        <name type="common">BtCoV</name>
        <name type="synonym">BtCoV/133/2005</name>
        <dbReference type="NCBI Taxonomy" id="389230"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Nidovirales</taxon>
            <taxon>Cornidovirineae</taxon>
            <taxon>Coronaviridae</taxon>
            <taxon>Orthocoronavirinae</taxon>
            <taxon>Betacoronavirus</taxon>
            <taxon>Merbecovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Tylonycteris pachypus</name>
        <name type="common">Lesser bamboo bat</name>
        <name type="synonym">Vespertilio pachypus</name>
        <dbReference type="NCBI Taxonomy" id="258959"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="7481" last="7490">
            <title>Prevalence and genetic diversity of coronaviruses in bats from China.</title>
            <authorList>
                <person name="Tang X.C."/>
                <person name="Zhang J.X."/>
                <person name="Zhang S.Y."/>
                <person name="Wang P."/>
                <person name="Fan X.H."/>
                <person name="Li L.F."/>
                <person name="Li G."/>
                <person name="Dong B.Q."/>
                <person name="Liu W."/>
                <person name="Cheung C.L."/>
                <person name="Xu K.M."/>
                <person name="Song W.J."/>
                <person name="Vijaykrishna D."/>
                <person name="Poon L.L.M."/>
                <person name="Peiris J.S.M."/>
                <person name="Smith G.J."/>
                <person name="Chen H."/>
                <person name="Guan Y."/>
            </authorList>
            <dbReference type="PubMed" id="16840328"/>
            <dbReference type="DOI" id="10.1128/JVI.00697-06"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homomultimer. Interacts with envelope E protein in the budding compartment of the host cell, which is located between endoplasmic reticulum and the Golgi complex. Forms a complex with HE and S proteins. Interacts with nucleocapsid N protein. This interaction probably participates in RNA packaging into the virus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">Largely embedded in the lipid bilayer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the betacoronaviruses M protein family.</text>
    </comment>
    <dbReference type="EMBL" id="DQ648794">
        <property type="protein sequence ID" value="ABG47057.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007449">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044178">
        <property type="term" value="C:host cell Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019031">
        <property type="term" value="C:viral envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039660">
        <property type="term" value="F:structural constituent of virion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21567">
        <property type="entry name" value="MERS-like-CoV_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04202">
        <property type="entry name" value="BETA_CORONA_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002574">
        <property type="entry name" value="M_CoV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044363">
        <property type="entry name" value="M_MERS-like-CoV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01635">
        <property type="entry name" value="CoV_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51927">
        <property type="entry name" value="COV_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0468">Viral matrix protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000290330" description="Membrane protein">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="1">
        <location>
            <begin position="71"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="1">
        <location>
            <begin position="100"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01275"/>
        </source>
    </evidence>
    <sequence length="219" mass="24690" checksum="AAB75B4D8BA32ED8" modified="2006-09-05" version="1">MSSNGSLTKDEVVNIIKDWNFSWSIIFLLITIVLQYGYPSRSMMVYVFKMFILWLLWPASMALSIFSAIYPISLASQIISGILAAICAVMWLAYFVQSIRLFMRTGSWWSFNPESNCLLNVPIGGTTVVRPLVEDSTSVTAVVNDGHLKMAGMHFGRCDYDRLPMEITVAKPSVLIALKMVKRQSYGTNSGVAIFHRYKAGNYRRPTIIQDEELALLRA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q0Q4E8</accession>
    <name>VEMP_BC133</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Envelope small membrane protein</fullName>
            <shortName evidence="1">E protein</shortName>
            <shortName evidence="1">sM protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">E</name>
        <name type="synonym">sM</name>
        <name type="ORF">4</name>
    </gene>
    <organism>
        <name type="scientific">Bat coronavirus 133/2005</name>
        <name type="common">BtCoV</name>
        <name type="synonym">BtCoV/133/2005</name>
        <dbReference type="NCBI Taxonomy" id="389230"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Nidovirales</taxon>
            <taxon>Cornidovirineae</taxon>
            <taxon>Coronaviridae</taxon>
            <taxon>Orthocoronavirinae</taxon>
            <taxon>Betacoronavirus</taxon>
            <taxon>Merbecovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Tylonycteris pachypus</name>
        <name type="common">Lesser bamboo bat</name>
        <name type="synonym">Vespertilio pachypus</name>
        <dbReference type="NCBI Taxonomy" id="258959"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="7481" last="7490">
            <title>Prevalence and genetic diversity of coronaviruses in bats from China.</title>
            <authorList>
                <person name="Tang X.C."/>
                <person name="Zhang J.X."/>
                <person name="Zhang S.Y."/>
                <person name="Wang P."/>
                <person name="Fan X.H."/>
                <person name="Li L.F."/>
                <person name="Li G."/>
                <person name="Dong B.Q."/>
                <person name="Liu W."/>
                <person name="Cheung C.L."/>
                <person name="Xu K.M."/>
                <person name="Song W.J."/>
                <person name="Vijaykrishna D."/>
                <person name="Poon L.L.M."/>
                <person name="Peiris J.S.M."/>
                <person name="Smith G.J."/>
                <person name="Chen H."/>
                <person name="Guan Y."/>
            </authorList>
            <dbReference type="PubMed" id="16840328"/>
            <dbReference type="DOI" id="10.1128/JVI.00697-06"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homopentamer. Interacts with membrane protein M in the budding compartment of the host cell, which is located between endoplasmic reticulum and the Golgi complex. Interacts with Nucleoprotein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">The cytoplasmic tail functions as a Golgi complex-targeting signal.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the betacoronaviruses E protein family.</text>
    </comment>
    <dbReference type="EMBL" id="DQ648794">
        <property type="protein sequence ID" value="ABG47056.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q0Q4E8"/>
    <dbReference type="Proteomes" id="UP000007449">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044178">
        <property type="term" value="C:host cell Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044662">
        <property type="term" value="P:disruption by virus of host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039707">
        <property type="term" value="P:pore formation by virus in membrane of host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046760">
        <property type="term" value="P:viral budding from Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21533">
        <property type="entry name" value="MERS-CoV-like_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04204">
        <property type="entry name" value="BETA_CORONA_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044379">
        <property type="entry name" value="E_MERS-CoV-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043506">
        <property type="entry name" value="E_protein_bCoV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003873">
        <property type="entry name" value="E_protein_CoV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02723">
        <property type="entry name" value="CoV_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51926">
        <property type="entry name" value="COV_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0053">Apoptosis</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000290327" description="Envelope small membrane protein">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="1">
        <location>
            <begin position="38"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04204"/>
        </source>
    </evidence>
    <sequence length="82" mass="9282" checksum="7247F5A0328E1D21" modified="2006-09-05" version="1">MLPFVHEQIGTIIVNFFILTVVCAITLLVCLAVLTAIRLCVQCASGVNTLLFVPAFYIYNTGRNAYFKFQENRPPFPPEDWV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q0Q4E9</accession>
    <name>NS3D_BC133</name>
    <protein>
        <recommendedName>
            <fullName>Non-structural protein 3d</fullName>
            <shortName>ns3d</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Accessory protein 3d</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">3d</name>
    </gene>
    <organism>
        <name type="scientific">Bat coronavirus 133/2005</name>
        <name type="common">BtCoV</name>
        <name type="synonym">BtCoV/133/2005</name>
        <dbReference type="NCBI Taxonomy" id="389230"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Nidovirales</taxon>
            <taxon>Cornidovirineae</taxon>
            <taxon>Coronaviridae</taxon>
            <taxon>Orthocoronavirinae</taxon>
            <taxon>Betacoronavirus</taxon>
            <taxon>Merbecovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Tylonycteris pachypus</name>
        <name type="common">Lesser bamboo bat</name>
        <name type="synonym">Vespertilio pachypus</name>
        <dbReference type="NCBI Taxonomy" id="258959"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="7481" last="7490">
            <title>Prevalence and genetic diversity of coronaviruses in bats from China.</title>
            <authorList>
                <person name="Tang X.C."/>
                <person name="Zhang J.X."/>
                <person name="Zhang S.Y."/>
                <person name="Wang P."/>
                <person name="Fan X.H."/>
                <person name="Li L.F."/>
                <person name="Li G."/>
                <person name="Dong B.Q."/>
                <person name="Liu W."/>
                <person name="Cheung C.L."/>
                <person name="Xu K.M."/>
                <person name="Song W.J."/>
                <person name="Vijaykrishna D."/>
                <person name="Poon L.L.M."/>
                <person name="Peiris J.S.M."/>
                <person name="Smith G.J."/>
                <person name="Chen H."/>
                <person name="Guan Y."/>
            </authorList>
            <dbReference type="PubMed" id="16840328"/>
            <dbReference type="DOI" id="10.1128/JVI.00697-06"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Host membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DQ648794">
        <property type="protein sequence ID" value="ABG47055.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007449">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033644">
        <property type="term" value="C:host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21645">
        <property type="entry name" value="MERS-CoV-like_ORF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044323">
        <property type="entry name" value="MERS-CoV-like_ORF5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51967">
        <property type="entry name" value="COV_VIROPORIN_3A_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51966">
        <property type="entry name" value="COV_VIROPORIN_3A_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000290270" description="Non-structural protein 3d">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="CoV 3a-like viroporin TM" evidence="2">
        <location>
            <begin position="27"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="CoV 3a-like viroporin CD" evidence="3">
        <location>
            <begin position="134"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="227" mass="25399" checksum="9EE7E3F66C38D958" modified="2006-09-05" version="1">MAFSASLFRTKTVHTEDALCPRSAIQAEQPPNIIDCIPVAGYEAALVTNALFLLVLFVFNPLTCKGNWIKAILFYSLLLYNMILAIFLVIDTQHFVSALLLAYVVTFLILWTADRVRLSCAVGSVLPFVDMRSSYIRVDNGNSSVVVPMNHTKHWFIRNFEQSCHCENCFYIHSSSYVECTFISRLKKSILVSVCDFSLGGNVSTVFVPSSDKTVPLHIIAPSKLYV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q0Q4F0</accession>
    <name>NS3C_BC133</name>
    <protein>
        <recommendedName>
            <fullName>Non-structural protein 3c</fullName>
            <shortName>ns3c</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Accessory protein 3c</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">3c</name>
    </gene>
    <organism>
        <name type="scientific">Bat coronavirus 133/2005</name>
        <name type="common">BtCoV</name>
        <name type="synonym">BtCoV/133/2005</name>
        <dbReference type="NCBI Taxonomy" id="389230"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Nidovirales</taxon>
            <taxon>Cornidovirineae</taxon>
            <taxon>Coronaviridae</taxon>
            <taxon>Orthocoronavirinae</taxon>
            <taxon>Betacoronavirus</taxon>
            <taxon>Merbecovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Tylonycteris pachypus</name>
        <name type="common">Lesser bamboo bat</name>
        <name type="synonym">Vespertilio pachypus</name>
        <dbReference type="NCBI Taxonomy" id="258959"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="7481" last="7490">
            <title>Prevalence and genetic diversity of coronaviruses in bats from China.</title>
            <authorList>
                <person name="Tang X.C."/>
                <person name="Zhang J.X."/>
                <person name="Zhang S.Y."/>
                <person name="Wang P."/>
                <person name="Fan X.H."/>
                <person name="Li L.F."/>
                <person name="Li G."/>
                <person name="Dong B.Q."/>
                <person name="Liu W."/>
                <person name="Cheung C.L."/>
                <person name="Xu K.M."/>
                <person name="Song W.J."/>
                <person name="Vijaykrishna D."/>
                <person name="Poon L.L.M."/>
                <person name="Peiris J.S.M."/>
                <person name="Smith G.J."/>
                <person name="Chen H."/>
                <person name="Guan Y."/>
            </authorList>
            <dbReference type="PubMed" id="16840328"/>
            <dbReference type="DOI" id="10.1128/JVI.00697-06"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <dbReference type="EMBL" id="DQ648794">
        <property type="protein sequence ID" value="ABG47054.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007449">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="CDD" id="cd21652">
        <property type="entry name" value="ORF4b_HKU4-CoV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044319">
        <property type="entry name" value="ORF4b_HKU4-CoV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000290267" description="Non-structural protein 3c">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <sequence length="285" mass="32589" checksum="8C2AFFDA42368779" modified="2006-09-05" version="1">MSIVLRLLSVLKHQQNKMQPELSVSNYRLLDYSMEWSSSSVAALPTSSEKTVMMPAKATSSRKRHRKPKLQYAKRRFSPVNPNDLVLVRQEPTHCVRLVFPLSKRWIHFDGLVYSLARLNVSMTVDYHVTLALIHAPEAGECFGNLLHLTPLLKDCLLEFKKLCILGKTLTILASEWPFFTGVKKNKDNLTFPKAVEWLKEHGYEIYHSQLPLHMSLAKLHDLPQAQFTEAVGLCHYFDPREFALPCALEVVKIGGGKVNGRSIPLVRFPINNEFKFIPYLYQCA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q0Q4F1</accession>
    <name>NS3A_BC133</name>
    <protein>
        <recommendedName>
            <fullName>Non-structural protein 3a</fullName>
            <shortName>ns3a</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Accessory protein 3a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">3a</name>
    </gene>
    <organism>
        <name type="scientific">Bat coronavirus 133/2005</name>
        <name type="common">BtCoV</name>
        <name type="synonym">BtCoV/133/2005</name>
        <dbReference type="NCBI Taxonomy" id="389230"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Nidovirales</taxon>
            <taxon>Cornidovirineae</taxon>
            <taxon>Coronaviridae</taxon>
            <taxon>Orthocoronavirinae</taxon>
            <taxon>Betacoronavirus</taxon>
            <taxon>Merbecovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Tylonycteris pachypus</name>
        <name type="common">Lesser bamboo bat</name>
        <name type="synonym">Vespertilio pachypus</name>
        <dbReference type="NCBI Taxonomy" id="258959"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="7481" last="7490">
            <title>Prevalence and genetic diversity of coronaviruses in bats from China.</title>
            <authorList>
                <person name="Tang X.C."/>
                <person name="Zhang J.X."/>
                <person name="Zhang S.Y."/>
                <person name="Wang P."/>
                <person name="Fan X.H."/>
                <person name="Li L.F."/>
                <person name="Li G."/>
                <person name="Dong B.Q."/>
                <person name="Liu W."/>
                <person name="Cheung C.L."/>
                <person name="Xu K.M."/>
                <person name="Song W.J."/>
                <person name="Vijaykrishna D."/>
                <person name="Poon L.L.M."/>
                <person name="Peiris J.S.M."/>
                <person name="Smith G.J."/>
                <person name="Chen H."/>
                <person name="Guan Y."/>
            </authorList>
            <dbReference type="PubMed" id="16840328"/>
            <dbReference type="DOI" id="10.1128/JVI.00697-06"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <dbReference type="EMBL" id="DQ648794">
        <property type="protein sequence ID" value="ABG47053.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007449">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000290261" description="Non-structural protein 3a">
        <location>
            <begin position="20"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="91" mass="9910" checksum="4547DA17F82EFD68" modified="2006-09-05" version="1" precursor="true">MVSFNATAILLVLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGMYTNSAMSYDGTTIPFDRDGIVHQDHYTDTKPTPLSDVGFSV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q0Q4F2</accession>
    <name>SPIKE_BC133</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spike glycoprotein</fullName>
            <shortName evidence="2">S glycoprotein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">E2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Peplomer protein</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="2">Spike protein S1</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="2">Spike protein S2</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="2">Spike protein S2'</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="2" type="primary">S</name>
        <name type="ORF">2</name>
    </gene>
    <organism>
        <name type="scientific">Bat coronavirus 133/2005</name>
        <name type="common">BtCoV</name>
        <name type="synonym">BtCoV/133/2005</name>
        <dbReference type="NCBI Taxonomy" id="389230"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Nidovirales</taxon>
            <taxon>Cornidovirineae</taxon>
            <taxon>Coronaviridae</taxon>
            <taxon>Orthocoronavirinae</taxon>
            <taxon>Betacoronavirus</taxon>
            <taxon>Merbecovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Tylonycteris pachypus</name>
        <name type="common">Lesser bamboo bat</name>
        <name type="synonym">Vespertilio pachypus</name>
        <dbReference type="NCBI Taxonomy" id="258959"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="7481" last="7490">
            <title>Prevalence and genetic diversity of coronaviruses in bats from China.</title>
            <authorList>
                <person name="Tang X.C."/>
                <person name="Zhang J.X."/>
                <person name="Zhang S.Y."/>
                <person name="Wang P."/>
                <person name="Fan X.H."/>
                <person name="Li L.F."/>
                <person name="Li G."/>
                <person name="Dong B.Q."/>
                <person name="Liu W."/>
                <person name="Cheung C.L."/>
                <person name="Xu K.M."/>
                <person name="Song W.J."/>
                <person name="Vijaykrishna D."/>
                <person name="Poon L.L.M."/>
                <person name="Peiris J.S.M."/>
                <person name="Smith G.J."/>
                <person name="Chen H."/>
                <person name="Guan Y."/>
            </authorList>
            <dbReference type="PubMed" id="16840328"/>
            <dbReference type="DOI" id="10.1128/JVI.00697-06"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <molecule>Spike protein S1</molecule>
        <text evidence="2">Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.</text>
    </comment>
    <comment type="function">
        <molecule>Spike protein S2</molecule>
        <text evidence="2">Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.</text>
    </comment>
    <comment type="function">
        <molecule>Spike protein S2'</molecule>
        <text evidence="2">Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="2">Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion.</text>
    </comment>
    <comment type="domain">
        <text evidence="2">Fusion peptide 1 (FP1) and fusion peptide 2 (FP2) function cooperatively and have a membrane-ordering effect on lipid headgroups and shallow hydrophobic regions of target bilayers. They are considered as two domains of an extended, bipartite FP. The membrane-ordering activity is calcium-dependent and also dependent on correct folding, which is maintained by an internal disulfide bond in FP2.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Specific enzymatic cleavages in vivo yield mature proteins.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">Specific enzymatic cleavages in vivo yield mature proteins. The precursor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins. Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the betacoronaviruses spike protein family.</text>
    </comment>
    <dbReference type="EMBL" id="DQ648794">
        <property type="protein sequence ID" value="ABG47052.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q0Q4F2"/>
    <dbReference type="Proteomes" id="UP000007449">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044173">
        <property type="term" value="C:host cell endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019031">
        <property type="term" value="C:viral envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075509">
        <property type="term" value="P:endocytosis involved in viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039654">
        <property type="term" value="P:fusion of virus membrane with host endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019064">
        <property type="term" value="P:fusion of virus membrane with host plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046813">
        <property type="term" value="P:receptor-mediated virion attachment to host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21487">
        <property type="entry name" value="bat_HKU4-like_Spike_S1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21626">
        <property type="entry name" value="MERS-CoV-like_Spike_S1_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04099">
        <property type="entry name" value="BETA_CORONA_SPIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032500">
        <property type="entry name" value="bCoV_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042578">
        <property type="entry name" value="BETA_CORONA_SPIKE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043607">
        <property type="entry name" value="CoV_S1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043473">
        <property type="entry name" value="S2_sf_CoV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043002">
        <property type="entry name" value="Spike_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044337">
        <property type="entry name" value="Spike_S1_N_MERS-CoV-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018548">
        <property type="entry name" value="Spike_S1_RBD_bCoV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044378">
        <property type="entry name" value="Spike_S1_RBD_HKU4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036326">
        <property type="entry name" value="Spike_S1_RBD_sf_bCoV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002552">
        <property type="entry name" value="Spike_S2_CoV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043614">
        <property type="entry name" value="Spike_S2_CoV_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044873">
        <property type="entry name" value="Spike_S2_CoV_HR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044874">
        <property type="entry name" value="Spike_S2_CoV_HR2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16451">
        <property type="entry name" value="bCoV_S1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09408">
        <property type="entry name" value="bCoV_S1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19209">
        <property type="entry name" value="CoV_S1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01601">
        <property type="entry name" value="CoV_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19214">
        <property type="entry name" value="CoV_S2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111474">
        <property type="entry name" value="SSF111474"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143587">
        <property type="entry name" value="SSF143587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51921">
        <property type="entry name" value="BCOV_S1_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51922">
        <property type="entry name" value="BCOV_S1_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51923">
        <property type="entry name" value="COV_S2_HR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-1170">Fusion of virus membrane with host endosomal membrane</keyword>
    <keyword id="KW-1168">Fusion of virus membrane with host membrane</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0564">Palmitate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0843">Virulence</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000290318" description="Spike glycoprotein">
        <location>
            <begin position="13"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000290319" description="Spike protein S1">
        <location>
            <begin position="13"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000290320" description="Spike protein S2">
        <location>
            <begin position="750"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000444058" description="Spike protein S2'" evidence="2">
        <location>
            <begin position="885"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="13"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1296"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="1317"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="domain" description="BetaCoV S1-NTD" evidence="4">
        <location>
            <begin position="21"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="BetaCoV S1-CTD" evidence="3">
        <location>
            <begin position="386"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fusion peptide 1" evidence="2">
        <location>
            <begin position="885"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fusion peptide 2" evidence="2">
        <location>
            <begin position="904"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Heptad repeat 1" evidence="2">
        <location>
            <begin position="991"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Heptad repeat 2" evidence="2">
        <location>
            <begin position="1245"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1020"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1257"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="KxHxx" evidence="2">
        <location>
            <begin position="1348"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage" evidence="1">
        <location>
            <begin position="749"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage" evidence="2">
        <location>
            <begin position="884"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
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            <position position="132"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
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            <position position="172"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="492"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="624"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="723"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="762"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="773"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="784"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
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            <position position="869"/>
        </location>
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    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="1142"/>
        </location>
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    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
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            <position position="1145"/>
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    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="1172"/>
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    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
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    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
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    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
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            <position position="1255"/>
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    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="1276"/>
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    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="1287"/>
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            <begin position="191"/>
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    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="344"/>
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        <location>
            <begin position="388"/>
            <end position="412"/>
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    <feature type="disulfide bond" evidence="3">
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            <begin position="430"/>
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            <begin position="442"/>
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        <location>
            <begin position="909"/>
            <end position="922"/>
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    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01270"/>
        </source>
    </evidence>
    <sequence length="1350" mass="148870" checksum="33E051F5A646A74D" modified="2006-09-05" version="1" precursor="true"> </sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>