<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="http://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
 xsi:schemaLocation="http://uniprot.org/uniprot http://www.uniprot.org/docs/uniprot.xsd">
<entry dataset="Swiss-Prot" created="1986-07-21" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>P03319</accession>
    <accession>Q9IZS9</accession>
    <name>PR73_MMTVC</name>
    <protein>
        <recommendedName>
            <fullName>Protein PR73</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Superantigen</fullName>
            <shortName>Sag</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">sag</name>
    </gene>
    <organism>
        <name type="scientific">Mouse mammary tumor virus (strain C3H)</name>
        <name type="common">MMTV</name>
        <dbReference type="NCBI Taxonomy" id="11759"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Pararnavirae</taxon>
            <taxon>Artverviricota</taxon>
            <taxon>Revtraviricetes</taxon>
            <taxon>Ortervirales</taxon>
            <taxon>Retroviridae</taxon>
            <taxon>Orthoretrovirinae</taxon>
            <taxon>Betaretrovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Mus musculus</name>
        <name type="common">Mouse</name>
        <dbReference type="NCBI Taxonomy" id="10090"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1983" name="J. Virol." volume="47" first="495" last="504">
            <title>Nucleotide sequencing of an apparent proviral copy of env mRNA defines determinants of expression of the mouse mammary tumor virus env gene.</title>
            <authorList>
                <person name="Majors J.E."/>
                <person name="Varmus H.E."/>
            </authorList>
            <dbReference type="PubMed" id="6312081"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Virol." volume="74" first="8876" last="8883">
            <title>Genetics of mouse mammary tumor virus-induced mammary tumors: linkage of tumor induction to the gag gene.</title>
            <authorList>
                <person name="Hook L.M."/>
                <person name="Agafonova Y."/>
                <person name="Ross S.R."/>
                <person name="Turner S.J."/>
                <person name="Golovkina T.V."/>
            </authorList>
            <dbReference type="PubMed" id="10982330"/>
            <dbReference type="DOI" id="10.1128/JVI.74.19.8876-8883.2000"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1981" name="J. Virol." volume="37" first="226" last="238">
            <title>Regulatory and coding potential of the mouse mammary tumor virus long terminal redundancy.</title>
            <authorList>
                <person name="Donehower L.A."/>
                <person name="Huang A.L."/>
                <person name="Hager G.L."/>
            </authorList>
            <dbReference type="PubMed" id="6260976"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA] OF 122-319</scope>
    </reference>
    <comment type="function">
        <text>Superantigen.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>This protein is coded in the long terminal repeat (LTR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mouse mammary tumor virus PR73 superantigen family.</text>
    </comment>
    <dbReference type="EMBL" id="K00556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF228552">
        <property type="protein sequence ID" value="AAF31476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S26389">
        <property type="entry name" value="QQMVTM"/>
    </dbReference>
    <dbReference type="SMR" id="P03319"/>
    <dbReference type="Proteomes" id="UP000006540">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001213">
        <property type="entry name" value="MMTV_SAg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01054">
        <property type="entry name" value="MMTV_SAg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0735">Signal-anchor</keyword>
    <keyword id="KW-0766">Superantigen</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000125501" description="Protein PR73">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="66"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31476." evidence="3" ref="2">
        <original>V</original>
        <variation>M</variation>
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31476." evidence="3" ref="2">
        <original>LGL</original>
        <variation>WGP</variation>
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; no nucleotide entry." evidence="3" ref="3">
        <original>P</original>
        <variation>L</variation>
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; no nucleotide entry." evidence="3" ref="3">
        <original>A</original>
        <variation>P</variation>
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="319" mass="36853" checksum="0CE30773DC7329A2" modified="2011-01-11" version="3">MPRLQQKWLNSRECPTPRGEAAKGLFPTKDDPSAHKRVSPSDKDIFILCCKLGIALLCLGLLGEVAVRARRALTLDSFNSSSVQDYNLNNSENSTFLLRQGPQPTSSYKPHRFCPSEIEIRMLAKNYIFTNKTNPIGRLLVTMLRNESLSFSTIFTQIQKLEMGIENRKRRSTSIEEQVQGLLTTGLEVKKGKKSVFVKIGDRWWQPGTYRGPYIYRPTDAPLPYTGRYDLNWDRWVTVNGYKVLYRSLPFRERLARARPPWCMLSQEEKDDMKQQVHDYIYLGTGMHFWGKIFHTKEGTVAGLIEHYSAKTYGMSYYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1989-07-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>P11283</accession>
    <accession>Q9IZT3</accession>
    <name>POL_MMTVC</name>
    <protein>
        <recommendedName>
            <fullName>Gag-Pro-Pol polyprotein</fullName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Matrix protein p10</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Phosphorylated protein pp21</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein p3</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein p8</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein n</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Capsid protein p27</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Nucleocapsid protein-dUTPase</fullName>
                <shortName>NC-dUTPase</shortName>
                <ecNumber evidence="16">3.6.1.23</ecNumber>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protease</fullName>
                <ecNumber evidence="6">3.4.23.-</ecNumber>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Reverse transcriptase/ribonuclease H</fullName>
                <shortName>RT</shortName>
                <ecNumber evidence="7">2.7.7.49</ecNumber>
                <ecNumber evidence="7">2.7.7.7</ecNumber>
                <ecNumber evidence="8">3.1.26.4</ecNumber>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Integrase</fullName>
                <shortName>IN</shortName>
                <ecNumber evidence="19">2.7.7.-</ecNumber>
                <ecNumber evidence="19">3.1.-.-</ecNumber>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">gag-pro-pol</name>
    </gene>
    <organism>
        <name type="scientific">Mouse mammary tumor virus (strain C3H)</name>
        <name type="common">MMTV</name>
        <dbReference type="NCBI Taxonomy" id="11759"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Pararnavirae</taxon>
            <taxon>Artverviricota</taxon>
            <taxon>Revtraviricetes</taxon>
            <taxon>Ortervirales</taxon>
            <taxon>Retroviridae</taxon>
            <taxon>Orthoretrovirinae</taxon>
            <taxon>Betaretrovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Mus musculus</name>
        <name type="common">Mouse</name>
        <dbReference type="NCBI Taxonomy" id="10090"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2000" name="J. Virol." volume="74" first="8876" last="8883">
            <title>Genetics of mouse mammary tumor virus-induced mammary tumors: linkage of tumor induction to the gag gene.</title>
            <authorList>
                <person name="Hook L.M."/>
                <person name="Agafonova Y."/>
                <person name="Ross S.R."/>
                <person name="Turner S.J."/>
                <person name="Golovkina T.V."/>
            </authorList>
            <dbReference type="PubMed" id="10982330"/>
            <dbReference type="DOI" id="10.1128/JVI.74.19.8876-8883.2000"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1987" name="Proc. Natl. Acad. Sci. U.S.A." volume="84" first="4298" last="4302">
            <title>Two efficient ribosomal frameshifting events are required for synthesis of mouse mammary tumor virus gag-related polyproteins.</title>
            <authorList>
                <person name="Jacks T."/>
                <person name="Townsley K."/>
                <person name="Varmus H.E."/>
                <person name="Majors J."/>
            </authorList>
            <dbReference type="PubMed" id="3035577"/>
            <dbReference type="DOI" id="10.1073/pnas.84.12.4298"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 359-591 AND 857-970</scope>
        <scope>RIBOSOMAL FRAMESHIFT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1989" name="J. Virol." volume="63" first="2543" last="2549">
            <title>Analysis of gag proteins from mouse mammary tumor virus.</title>
            <authorList>
                <person name="Hizi A."/>
                <person name="Henderson L.E."/>
                <person name="Copeland T.D."/>
                <person name="Sowder R.C."/>
                <person name="Krutzsch H.C."/>
                <person name="Oroszlan S."/>
            </authorList>
            <dbReference type="PubMed" id="2542570"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-58; 65-116; 194-218; 227-241; 251-252; 270-276 AND 362-591</scope>
        <scope>PROTEOLYTIC CLEAVAGE (GAG-PRO-POL POLYPROTEIN)</scope>
        <scope>MYRISTOYLATION AT GLY-2</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1994" name="Virology" volume="204" first="420" last="424">
            <title>The protein p30, encoded at the gag-pro junction of mouse mammary tumor virus, is a dUTPase fused with a nucleocapsid protein.</title>
            <authorList>
                <person name="Bergman A.C."/>
                <person name="Bjornberg O."/>
                <person name="Nord J."/>
                <person name="Nyman P.O."/>
                <person name="Rosengren A.M."/>
            </authorList>
            <dbReference type="PubMed" id="8091672"/>
            <dbReference type="DOI" id="10.1006/viro.1994.1547"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 497-512 AND 744-745</scope>
        <scope>RIBOSOMAL FRAMESHIFT</scope>
        <scope>SUBUNIT (NUCLEOCAPSID PROTEIN-DUTPASE)</scope>
        <scope>FUNCTION (NUCLEOCAPSID PROTEIN-DUTPASE)</scope>
        <scope>COFACTOR (NUCLEOCAPSID PROTEIN-DUTPASE)</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2013" name="PLoS ONE" volume="8" first="E76638" last="E76638">
            <title>Biochemical characterization of novel retroviral integrase proteins.</title>
            <authorList>
                <person name="Ballandras-Colas A."/>
                <person name="Naraharisetty H."/>
                <person name="Li X."/>
                <person name="Serrao E."/>
                <person name="Engelman A."/>
            </authorList>
            <dbReference type="PubMed" id="24124581"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0076638"/>
        </citation>
        <scope>FUNCTION (INTEGRASE)</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2017" name="Curr. Opin. Struct. Biol." volume="47" first="23" last="29">
            <title>Retroviral intasomes arising.</title>
            <authorList>
                <person name="Engelman A.N."/>
                <person name="Cherepanov P."/>
            </authorList>
            <dbReference type="PubMed" id="28458055"/>
            <dbReference type="DOI" id="10.1016/j.sbi.2017.04.005"/>
        </citation>
        <scope>REVIEW (INTEGRASE)</scope>
    </reference>
    <comment type="function">
        <molecule>Matrix protein p10</molecule>
        <text evidence="18">Matrix protein.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Nucleocapsid protein p14: Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.</text>
    </comment>
    <comment type="function">
        <molecule>Capsid protein p27</molecule>
        <text evidence="18">Capsid protein.</text>
    </comment>
    <comment type="function">
        <text evidence="20">NC-dUTPase has dUTPase activity, thereby preventing incorporation of uracil into DNA.</text>
    </comment>
    <comment type="function">
        <molecule>Protease</molecule>
        <text evidence="6">The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.</text>
    </comment>
    <comment type="function">
        <molecule>Reverse transcriptase/ribonuclease H</molecule>
        <text evidence="7">RT is a multifunctional enzyme that converts the viral dimeric RNA genome into dsDNA in the cytoplasm, shortly after virus entry into the cell. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3' to 5' endonucleasic mode. Conversion of viral genomic RNA into dsDNA requires many steps. A tRNA binds to the primer-binding site (PBS) situated at the 5' end of the viral RNA. RT uses the 3' end of the tRNA primer to perfom a short round of RNA-dependent minus-strand DNA synthesis. The reading proceeds through the U5 region and ends after the repeated (R) region which is present at both ends of viral RNA. The portion of the RNA-DNA heteroduplex is digested by the RNase H, resulting in a ssDNA product attached to the tRNA primer. This ssDNA/tRNA hybridizes with the identical R region situated at the 3' end of viral RNA. This template exchange, known as minus-strand DNA strong stop transfer, can be either intra- or intermolecular. RT uses the 3' end of this newly synthesized short ssDNA to perfom the RNA-dependent minus-strand DNA synthesis of the whole template. RNase H digests the RNA template except for a polypurine tract (PPT) situated at the 5' end of the genome. It is not clear if both polymerase and RNase H activities are simultaneous. RNase H probably can proceed both in a polymerase-dependent (RNA cut into small fragments by the same RT performing DNA synthesis) and a polymerase-independent mode (cleavage of remaining RNA fragments by free RTs). Secondly, RT performs DNA-directed plus-strand DNA synthesis using the PPT that has not been removed by RNase H as primers. PPT and tRNA primers are then removed by RNase H. The 3' and 5' ssDNA PBS regions hybridize to form a circular dsDNA intermediate. Strand displacement synthesis by RT to the PBS and PPT ends produces a blunt ended, linear dsDNA copy of the viral genome that includes long terminal repeats (LTRs) at both ends.</text>
    </comment>
    <comment type="function">
        <molecule>Integrase</molecule>
        <text evidence="13 17">Catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.49"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>dUTP + H2O = diphosphate + dUMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">The RT polymerase active site binds 2 magnesium ions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="20">Magnesium ions are required for NC-dUTPase activity.</text>
    </comment>
    <comment type="subunit">
        <molecule>Matrix protein p10</molecule>
        <text evidence="2">Homodimer; when myristoylated.</text>
    </comment>
    <comment type="subunit">
        <molecule>Protease</molecule>
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subunit">
        <molecule>Integrase</molecule>
        <text evidence="17">Homooctamer.</text>
    </comment>
    <comment type="subunit">
        <molecule>Nucleocapsid protein-dUTPase</molecule>
        <text evidence="16 17">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Matrix protein p10</molecule>
        <subcellularLocation>
            <location evidence="3">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Capsid protein p27</molecule>
        <subcellularLocation>
            <location evidence="3">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Nucleocapsid protein-dUTPase</molecule>
        <subcellularLocation>
            <location evidence="3">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="ribosomal frameshifting"/>
        <isoform>
            <id>P11283-1</id>
            <name>Gag-Pro-Pol polyprotein</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q9IZT2-1</id>
            <name>Gag-Pro polyprotein</name>
            <sequence type="external"/>
        </isoform>
        <isoform>
            <id>P11284-1</id>
            <name>Gag polyprotein</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <comment type="domain">
        <molecule>Gag-Pro-Pol polyprotein</molecule>
        <text evidence="18">Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Gag-p27 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101.</text>
    </comment>
    <comment type="PTM">
        <molecule>Protease</molecule>
        <text evidence="2">Released by autocatalytic processing.</text>
    </comment>
    <comment type="PTM">
        <molecule>Gag-Pro-Pol polyprotein</molecule>
        <text evidence="14">Specific enzymatic cleavages in vivo yield mature proteins.</text>
    </comment>
    <comment type="PTM">
        <molecule>Gag-Pro-Pol polyprotein</molecule>
        <text evidence="14">Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.</text>
    </comment>
    <comment type="miscellaneous">
        <molecule>Reverse transcriptase/ribonuclease H</molecule>
        <text evidence="7">The reverse transcriptase is an error-prone enzyme that lacks a proof-reading function. High mutations rate is a direct consequence of this characteristic. RT also displays frequent template swiching leading to high recombination rate. Recombination mostly occurs between homologous regions of the two copackaged RNA genomes. If these two RNA molecules derive from different viral strains, reverse transcription will give rise to highly recombinated proviral DNAs.</text>
    </comment>
    <comment type="miscellaneous">
        <molecule>Isoform Gag-Pro-Pol polyprotein</molecule>
        <text evidence="15 16">Produced by -1 ribosomal frameshiftings between gag-pro and pro-pol.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="16"/>
    <dbReference type="EC" id="3.4.23.-" evidence="6"/>
    <dbReference type="EC" id="2.7.7.49" evidence="7"/>
    <dbReference type="EC" id="2.7.7.7" evidence="7"/>
    <dbReference type="EC" id="3.1.26.4" evidence="8"/>
    <dbReference type="EC" id="2.7.7.-" evidence="19"/>
    <dbReference type="EC" id="3.1.-.-" evidence="19"/>
    <dbReference type="EMBL" id="AF228552">
        <property type="protein sequence ID" value="AAF31474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M16766">
        <property type="protein sequence ID" value="AAA66623.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M16766">
        <property type="protein sequence ID" value="AAA66625.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="B29029">
        <property type="entry name" value="B29029"/>
    </dbReference>
    <dbReference type="BMRB" id="P11283"/>
    <dbReference type="SMR" id="P11283"/>
    <dbReference type="MEROPS" id="A02.010"/>
    <dbReference type="iPTMnet" id="P11283"/>
    <dbReference type="Proteomes" id="UP000006540">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019013">
        <property type="term" value="C:viral nucleocapsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039660">
        <property type="term" value="F:structural constituent of virion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075713">
        <property type="term" value="P:establishment of integrated proviral latency"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046718">
        <property type="term" value="P:viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044826">
        <property type="term" value="P:viral genome integration into host DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05482">
        <property type="entry name" value="HIV_retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.375.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003322">
        <property type="entry name" value="B_retro_matrix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038124">
        <property type="entry name" value="B_retro_matrix_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045345">
        <property type="entry name" value="Gag_p24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000721">
        <property type="entry name" value="Gag_p24_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036862">
        <property type="entry name" value="Integrase_C_dom_sf_retrovir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001037">
        <property type="entry name" value="Integrase_C_retrovir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003308">
        <property type="entry name" value="Integrase_Zn-bd_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034170">
        <property type="entry name" value="Retropepsin-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018061">
        <property type="entry name" value="Retropepsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008916">
        <property type="entry name" value="Retrov_capsid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008919">
        <property type="entry name" value="Retrov_capsid_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010999">
        <property type="entry name" value="Retrovr_matrix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010661">
        <property type="entry name" value="RVT_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02337">
        <property type="entry name" value="Gag_p10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00607">
        <property type="entry name" value="Gag_p24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19317">
        <property type="entry name" value="Gag_p24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00552">
        <property type="entry name" value="IN_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02022">
        <property type="entry name" value="Integrase_Zn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00077">
        <property type="entry name" value="RVP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06817">
        <property type="entry name" value="RVT_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46919">
        <property type="entry name" value="SSF46919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47836">
        <property type="entry name" value="SSF47836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47943">
        <property type="entry name" value="SSF47943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50122">
        <property type="entry name" value="SSF50122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="SSF50630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="SSF57756"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51027">
        <property type="entry name" value="INTEGRASE_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50876">
        <property type="entry name" value="ZF_INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0064">Aspartyl protease</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0229">DNA integration</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0519">Myristate</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0688">Ribosomal frameshifting</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-1179">Viral genome integration</keyword>
    <keyword id="KW-0468">Viral matrix protein</keyword>
    <keyword id="KW-0543">Viral nucleoprotein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="initiator methionine" description="Removed; by host" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000125493" description="Gag-Pro-Pol polyprotein">
        <location>
            <begin position="2"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403612" description="Matrix protein p10">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403613" description="Phosphorylated protein pp21">
        <location>
            <begin position="100"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403614" description="Protein p3">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403615" description="Protein p8">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403616" description="Protein n">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403617" description="Capsid protein p27">
        <location>
            <begin position="270"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403618" description="Nucleocapsid protein-dUTPase">
        <location>
            <begin position="497"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403619" description="Protease">
        <location>
            <begin position="746"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403620" description="Reverse transcriptase/ribonuclease H">
        <location>
            <begin position="861"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403621" description="Integrase">
        <location>
            <begin position="1438"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A2" evidence="6">
        <location>
            <begin position="766"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="7">
        <location>
            <begin position="905"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="8">
        <location>
            <begin position="1307"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="1490"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CCHC-type 1" evidence="5">
        <location>
            <begin position="525"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CCHC-type 2" evidence="5">
        <location>
            <begin position="552"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="Integrase-type" evidence="9">
        <location>
            <begin position="1436"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Integrase-type" evidence="11">
        <location>
            <begin position="1653"/>
            <end position="1702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="154"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="572"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1699"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PTAP/PSAP motif" evidence="18">
        <location>
            <begin position="305"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="581"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1715"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="active site" description="Protease; shared with dimeric partner" evidence="6">
        <location>
            <position position="771"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="970"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic; for reverse transcriptase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="1045"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic; for reverse transcriptase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="1046"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic; for reverse transcriptase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="1316"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic; for RNase H activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="1346"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic; for RNase H activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="1366"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic; for RNase H activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="1429"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic; for RNase H activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="1445"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="1449"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="1473"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="1476"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="1501"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
            <note>catalytic; for integrase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="1558"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
            <note>catalytic; for integrase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1594"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
            <note>catalytic; for integrase activity</note>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="14">
        <location>
            <begin position="99"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="14">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="14">
        <location>
            <begin position="228"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="14">
        <location>
            <begin position="254"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="14">
        <location>
            <begin position="269"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="14">
        <location>
            <begin position="496"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="16">
        <location>
            <begin position="745"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="16">
        <location>
            <begin position="1437"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="N-myristoyl glycine; by host" evidence="14">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="sequence variant">
        <original>E</original>
        <variation>K</variation>
        <location>
            <position position="523"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAA66625." evidence="18" ref="2">
        <original>SQDL</original>
        <variation>FTGF</variation>
        <location>
            <begin position="857"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAA66625." evidence="18" ref="2">
        <original>S</original>
        <variation>T</variation>
        <location>
            <position position="867"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAA66625." evidence="18" ref="2">
        <original>P</original>
        <variation>L</variation>
        <location>
            <position position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P03354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P03365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="P10258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4"/>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="13">
        <source>
            <dbReference type="PubMed" id="24124581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="14">
        <source>
            <dbReference type="PubMed" id="2542570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="15">
        <source>
            <dbReference type="PubMed" id="3035577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="16">
        <source>
            <dbReference type="PubMed" id="8091672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="17">
        <source>
            <dbReference type="PubMed" id="28458055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="18"/>
    <evidence type="ECO:0000305" key="19">
        <source>
            <dbReference type="PubMed" id="24124581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="20">
        <source>
            <dbReference type="PubMed" id="8091672"/>
        </source>
    </evidence>
    <sequence length="1755" mass="197329" checksum="0E99A2ADD96823A4" modified="2011-01-11" version="2">MGVSGSKGQKLFVSVLQRLLSERGLHVKESSAIEFYQFLIKVSPWFPEEGGLNLQDWKRVGREMKKYAAEHGTDSIPKQAYPIWLQLREILTEQSDLVLLSAEAKSVTEEELEEGLTGLLSASSQEKTYGTRGTAYAEIDTEVDKLSEHIYDEPYEEKEKADKNEEKDHVRKVKKIVQRKENSEHKRKEKDQKAFLATDWNNDDLSPEDWDDLEEQAAHYHDDDELILPVKRKVDKKKPLALRRKPLPPVGFAGAMAEAREKGDLTFTFPVVFMGESDDDDTPVWEPLPLKTLKELQSAVRTMGPSAPYTLQVVDMVASQWLTPSDWHQTARATLSPGDYVLWRTEYEEKSKETVQKTAGKRKGKVSLDMLLGTGQFLSPSSQIKLSKDVLKDVTTNAVLAWRAIPPPGVKKTVLAGLKQGNEESYETFISRLEEAVYRMMPRGEGSDILIKQLAWENANSLCQDLIRPMRKTGTMQDYIRACLDASPAVVQGMAYAAAMRGQKYSTFVKQTYGGGKGGQGSEGPVCFSCGKTGHIKRDCKEEKGSKRAPPGLCPRCKKGYHWKSECKSKFDKDGNPLPPLETNAENSKNLVKGQSPSPTQKGDKGKDSGLNPEAPPFTIHDLPRGTPGSAGLDLSSQKDLILSLEDGVSLVPTLVKGTLPEGTTGLIIGRSSNYKKGLEVLPGVIDSDFQGEIKVMVKAAKNAVIIHKGERIAQLLLLPYLKLPNPIIKEERGSEGFGSTSHVHWVQEISDSRPMLHISLNGRRFLGLLDTGADKTCIAGRDWPANWPIHQTESSLQGLGMACGVARSSQPLRWQHEDKSGIIHPFVIPTLPFTLWGRDIMKEIKVRLMTDSPDDSQDLMIGAIESNLFADQISWKSDQPVWLNQWPLKQEKLQALQQLVTEQLQLGHLEESNSPWNTPVFVIKKKSGKWRLLQDLRAVNATMHDMGALQPGLPSPVAVPKGWEIIIIDLQDCFFNIKLHPEDCKRFAFSVPSPNFKRPYQRFQWKVLPQGMKNSPTLCQKFVDKAILTVRDKYQDSYIVHYMDDILLAHPSRSIVDEILTSMIQALNKHGLVVSTEKIQKYDNLKYLGTHIQGDAVSYQKLQIRTDKLRTLNDFQKLLGNINWIRPFLKLTTGELKPLFEILNGDSNPISIRKLTPEACKALQLVNERLSIARVKRLDLSRPWSLCILKTEYTPTACLWQNGVLEWIHLPHISPKVITPYDIFCTQLIIKGRHRSKELFSKDPDYIVVPYTKVQFDLLLQEKEDWPISLLGFLGEVHFHLPKDPLLTFTLQTAIIFPHMTSTTPLEKGIVIFTDGSANGRSVTYIQGREPIIKENTQNTAQQAEIVAVITAFEEVSQSFNLYTDSKYVTGLFPEIETATLSPRTKIYTELRHLQRLIHKRQEKFYIGHIRGHTGLPGPLAQGNAYADSLTRILTALESAQESHALHHQNAAALRFQFHITREQAREIVKLCPNCPDWGHAPQLGVNPRGLKPRVLWQMDVTHVSEFGKLKYVHVTVDTYSHFTFATARTGEATKDVLQHLAQSFAYMGFPQKIKTDNAPAYVSRSIQEFLARWKISHVTGIPYNPQGQAIVERTHQNIKAQLNKLQKAGKYYTPHHLLAHALFVLNHVNMDNQGHTAAERHWGPISADPKPMVMWKDLLAGSWKGPDVLITAGRGYACVFPQDAETPIWVPDRFIRPFTERKEATPTPGTAEKTPPRDEKDQQKSPEDESSPHQREDGLATSAGVNLRSGGGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1989-07-01" modified="2022-02-23" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>P11284</accession>
    <accession>Q9IZT1</accession>
    <name>GAG_MMTVC</name>
    <protein>
        <recommendedName>
            <fullName>Gag polyprotein</fullName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Matrix protein p10</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Phosphorylated protein pp21</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein p3</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein p8</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein n</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Capsid protein p27</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Nucleocapsid protein p14</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">gag</name>
    </gene>
    <organism>
        <name type="scientific">Mouse mammary tumor virus (strain C3H)</name>
        <name type="common">MMTV</name>
        <dbReference type="NCBI Taxonomy" id="11759"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Pararnavirae</taxon>
            <taxon>Artverviricota</taxon>
            <taxon>Revtraviricetes</taxon>
            <taxon>Ortervirales</taxon>
            <taxon>Retroviridae</taxon>
            <taxon>Orthoretrovirinae</taxon>
            <taxon>Betaretrovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Mus musculus</name>
        <name type="common">Mouse</name>
        <dbReference type="NCBI Taxonomy" id="10090"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2000" name="J. Virol." volume="74" first="8876" last="8883">
            <title>Genetics of mouse mammary tumor virus-induced mammary tumors: linkage of tumor induction to the gag gene.</title>
            <authorList>
                <person name="Hook L.M."/>
                <person name="Agafonova Y."/>
                <person name="Ross S.R."/>
                <person name="Turner S.J."/>
                <person name="Golovkina T.V."/>
            </authorList>
            <dbReference type="PubMed" id="10982330"/>
            <dbReference type="DOI" id="10.1128/JVI.74.19.8876-8883.2000"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1987" name="Proc. Natl. Acad. Sci. U.S.A." volume="84" first="4298" last="4302">
            <title>Two efficient ribosomal frameshifting events are required for synthesis of mouse mammary tumor virus gag-related polyproteins.</title>
            <authorList>
                <person name="Jacks T."/>
                <person name="Townsley K."/>
                <person name="Varmus H.E."/>
                <person name="Majors J."/>
            </authorList>
            <dbReference type="PubMed" id="3035577"/>
            <dbReference type="DOI" id="10.1073/pnas.84.12.4298"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 359-591 AND 857-970</scope>
        <scope>RIBOSOMAL FRAMESHIFT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1989" name="J. Virol." volume="63" first="2543" last="2549">
            <title>Analysis of gag proteins from mouse mammary tumor virus.</title>
            <authorList>
                <person name="Hizi A."/>
                <person name="Henderson L.E."/>
                <person name="Copeland T.D."/>
                <person name="Sowder R.C."/>
                <person name="Krutzsch H.C."/>
                <person name="Oroszlan S."/>
            </authorList>
            <dbReference type="PubMed" id="2542570"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-58; 65-116; 194-218; 227-241; 251-252; 270-276 AND 362-591</scope>
        <scope>PROTEOLYTIC CLEAVAGE (GAG POLYPROTEIN)</scope>
        <scope>MYRISTOYLATION AT GLY-2</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="Biochemistry" volume="39" first="1604" last="1612">
            <title>The NMR structure of the nucleocapsid protein from the mouse mammary tumor virus reveals unusual folding of the C-terminal zinc knuckle.</title>
            <authorList>
                <person name="Klein D.J."/>
                <person name="Johnson P.E."/>
                <person name="Zollars E.S."/>
                <person name="De Guzman R.N."/>
                <person name="Summers M.F."/>
            </authorList>
            <dbReference type="PubMed" id="10677209"/>
            <dbReference type="DOI" id="10.1021/bi9922493"/>
        </citation>
        <scope>STRUCTURE BY NMR OF 523-543 AND 550-580</scope>
    </reference>
    <comment type="function">
        <molecule>Matrix protein p10</molecule>
        <text evidence="6">Matrix protein.</text>
    </comment>
    <comment type="function">
        <molecule>Nucleocapsid protein p14</molecule>
        <text evidence="6">Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.</text>
    </comment>
    <comment type="function">
        <molecule>Capsid protein p27</molecule>
        <text evidence="6">Capsid protein.</text>
    </comment>
    <comment type="subunit">
        <molecule>Matrix protein p10</molecule>
        <text evidence="1">Homodimer; when myristoylated.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Matrix protein p10</molecule>
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Capsid protein p27</molecule>
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Nucleocapsid protein p14</molecule>
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="ribosomal frameshifting"/>
        <isoform>
            <id>P11284-1</id>
            <name>Gag polyprotein</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q9IZT2-1</id>
            <name>Gag-Pro polyprotein</name>
            <sequence type="external"/>
        </isoform>
        <isoform>
            <id>P11283-1</id>
            <name>Gag-Pro-Pol polyprotein</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <comment type="domain">
        <molecule>Gag polyprotein</molecule>
        <text evidence="6">Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Gag-p27 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101.</text>
    </comment>
    <comment type="PTM">
        <molecule>Gag polyprotein</molecule>
        <text evidence="4">Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.</text>
    </comment>
    <comment type="PTM">
        <molecule>Gag polyprotein</molecule>
        <text evidence="4">Specific enzymatic cleavages in vivo yield mature proteins.</text>
    </comment>
    <comment type="miscellaneous">
        <molecule>Isoform Gag polyprotein</molecule>
        <text evidence="5">Produced by conventional translation.</text>
    </comment>
    <dbReference type="EMBL" id="AF228552">
        <property type="protein sequence ID" value="AAF31472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M16766">
        <property type="protein sequence ID" value="AAA66623.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="A29029">
        <property type="entry name" value="A29029"/>
    </dbReference>
    <dbReference type="PDB" id="1DSQ">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=523-543"/>
    </dbReference>
    <dbReference type="PDB" id="1DSV">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=550-580"/>
    </dbReference>
    <dbReference type="PDBsum" id="1DSQ"/>
    <dbReference type="PDBsum" id="1DSV"/>
    <dbReference type="BMRB" id="P11284"/>
    <dbReference type="SMR" id="P11284"/>
    <dbReference type="ELM" id="P11284"/>
    <dbReference type="iPTMnet" id="P11284"/>
    <dbReference type="EvolutionaryTrace" id="P11284"/>
    <dbReference type="Proteomes" id="UP000006540">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.375.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003322">
        <property type="entry name" value="B_retro_matrix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038124">
        <property type="entry name" value="B_retro_matrix_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045345">
        <property type="entry name" value="Gag_p24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000721">
        <property type="entry name" value="Gag_p24_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008916">
        <property type="entry name" value="Retrov_capsid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008919">
        <property type="entry name" value="Retrov_capsid_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010999">
        <property type="entry name" value="Retrovr_matrix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02337">
        <property type="entry name" value="Gag_p10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00607">
        <property type="entry name" value="Gag_p24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19317">
        <property type="entry name" value="Gag_p24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47836">
        <property type="entry name" value="SSF47836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47943">
        <property type="entry name" value="SSF47943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="SSF57756"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0519">Myristate</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0688">Ribosomal frameshifting</keyword>
    <keyword id="KW-1198">Viral budding</keyword>
    <keyword id="KW-1187">Viral budding via the host ESCRT complexes</keyword>
    <keyword id="KW-0468">Viral matrix protein</keyword>
    <keyword id="KW-0543">Viral nucleoprotein</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="initiator methionine" description="Removed; by host" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000442466" description="Gag polyprotein">
        <location>
            <begin position="2"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000040927" description="Matrix protein p10">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000040928" description="Phosphorylated protein pp21">
        <location>
            <begin position="100"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000040929" description="Protein p3">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000040930" description="Protein p8">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000040931" description="Protein n">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000040932" description="Capsid protein p27">
        <location>
            <begin position="270"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000040933" description="Nucleocapsid protein p14">
        <location>
            <begin position="497"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CCHC-type 1" evidence="2">
        <location>
            <begin position="525"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CCHC-type 2" evidence="2">
        <location>
            <begin position="552"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="154"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="568"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PTAP/PSAP motif" evidence="6">
        <location>
            <begin position="305"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="4">
        <location>
            <begin position="99"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="4">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="4">
        <location>
            <begin position="228"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="1">
        <location>
            <begin position="254"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="4">
        <location>
            <begin position="269"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="4">
        <location>
            <begin position="496"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="N-myristoyl glycine; by host" evidence="4">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="sequence variant">
        <original>K</original>
        <variation>E</variation>
        <location>
            <position position="523"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="6" ref="3">
        <original>S</original>
        <variation>T</variation>
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="6" ref="3">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAF31472." evidence="6" ref="1">
        <original>V</original>
        <variation>M</variation>
        <location>
            <position position="440"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="528"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="533"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="537"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="555"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="559"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="564"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="570"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="573"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P10258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="2542570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="3035577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="1DSQ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="1DSV"/>
        </source>
    </evidence>
    <sequence length="591" mass="66351" checksum="8F9E3C05D6F7B9AC" modified="2007-01-23" version="3">MGVSGSKGQKLFVSVLQRLLSERGLHVKESSAIEFYQFLIKVSPWFPEEGGLNLQDWKRVGREMKKYAAEHGTDSIPKQAYPIWLQLREILTEQSDLVLLSAEAKSVTEEELEEGLTGLLSASSQEKTYGTRGTAYAEIDTEVDKLSEHIYDEPYEEKEKADKNEEKDHVRKVKKIVQRKENSEHKRKEKDQKAFLATDWNNDDLSPEDWDDLEEQAAHYHDDDELILPVKRKVDKKKPLALRRKPLPPVGFAGAMAEAREKGDLTFTFPVVFMGESDDDDTPVWEPLPLKTLKELQSAVRTMGPSAPYTLQVVDMVASQWLTPSDWHQTARATLSPGDYVLWRTEYEEKSKETVQKTAGKRKGKVSLDMLLGTGQFLSPSSQIKLSKDVLKDVTTNAVLAWRAIPPPGVKKTVLAGLKQGNEESYETFISRLEEAVYRVMPRGEGSDILIKQLAWENANSLCQDLIRPMRKTGTMQDYIRACLDASPAVVQGMAYAAAMRGQKYSTFVKQTYGGGKGGQGSKGPVCFSCGKTGHIKRDCKEEKGSKRAPPGLCPRCKKGYHWKSECKSKFDKDGNPLPPLETNAENSKNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-07-19" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q85646</accession>
    <accession>Q9IZT0</accession>
    <name>ENV_MMTVC</name>
    <protein>
        <recommendedName>
            <fullName>Envelope glycoprotein gp70</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Env polyprotein</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Surface protein</fullName>
                <shortName>SU</shortName>
            </recommendedName>
            <alternativeName>
                <fullName>Glycoprotein 52</fullName>
                <shortName>gp52</shortName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Transmembrane protein</fullName>
                <shortName>TM</shortName>
            </recommendedName>
            <alternativeName>
                <fullName>Glycoprotein 36</fullName>
                <shortName>gp36</shortName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name type="primary">env</name>
    </gene>
    <organism>
        <name type="scientific">Mouse mammary tumor virus (strain C3H)</name>
        <name type="common">MMTV</name>
        <dbReference type="NCBI Taxonomy" id="11759"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Pararnavirae</taxon>
            <taxon>Artverviricota</taxon>
            <taxon>Revtraviricetes</taxon>
            <taxon>Ortervirales</taxon>
            <taxon>Retroviridae</taxon>
            <taxon>Orthoretrovirinae</taxon>
            <taxon>Betaretrovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Mus musculus</name>
        <name type="common">Mouse</name>
        <dbReference type="NCBI Taxonomy" id="10090"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1983" name="J. Virol." volume="47" first="495" last="504">
            <title>Nucleotide sequencing of an apparent proviral copy of env mRNA defines determinants of expression of the mouse mammary tumor virus env gene.</title>
            <authorList>
                <person name="Majors J.E."/>
                <person name="Varmus H.E."/>
            </authorList>
            <dbReference type="PubMed" id="6312081"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Virol." volume="74" first="8876" last="8883">
            <title>Genetics of mouse mammary tumor virus-induced mammary tumors: linkage of tumor induction to the gag gene.</title>
            <authorList>
                <person name="Hook L.M."/>
                <person name="Agafonova Y."/>
                <person name="Ross S.R."/>
                <person name="Turner S.J."/>
                <person name="Golovkina T.V."/>
            </authorList>
            <dbReference type="PubMed" id="10982330"/>
            <dbReference type="DOI" id="10.1128/JVI.74.19.8876-8883.2000"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1982" name="J. Virol." volume="41" first="414" last="422">
            <title>Processing and amino acid sequence analysis of the mouse mammary tumor virus env gene product.</title>
            <authorList>
                <person name="Arthur L.O."/>
                <person name="Copeland T.D."/>
                <person name="Oroszlan S."/>
                <person name="Schochetman G."/>
            </authorList>
            <dbReference type="PubMed" id="6281457"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 99-144</scope>
        <scope>PROTEOLYTIC PROCESSING OF POLYPROTEIN</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1983" name="J. Virol." volume="48" first="314" last="319">
            <title>Terminal amino acid sequences and proteolytic cleavage sites of mouse mammary tumor virus env gene products.</title>
            <authorList>
                <person name="Henderson L.E."/>
                <person name="Sowder R."/>
                <person name="Smythers G."/>
                <person name="Oroszlan S."/>
            </authorList>
            <dbReference type="PubMed" id="6310154"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 457-484</scope>
        <scope>PROTEOLYTIC PROCESSING OF POLYPROTEIN</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2003" name="J. Virol." volume="77" first="10468" last="10478">
            <title>Identification of the receptor binding domain of the mouse mammary tumor virus envelope protein.</title>
            <authorList>
                <person name="Zhang Y."/>
                <person name="Rassa J.C."/>
                <person name="deObaldia M.E."/>
                <person name="Albritton L.M."/>
                <person name="Ross S.R."/>
            </authorList>
            <dbReference type="PubMed" id="12970432"/>
            <dbReference type="DOI" id="10.1128/JVI.77.19.10468-10478.2003"/>
        </citation>
        <scope>MUTAGENESIS OF PHE-138 AND GLY-140</scope>
    </reference>
    <comment type="function">
        <text evidence="1">The surface protein (SU) attaches the virus to the host cell by binding to its receptor. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide. Fusion occurs at the host cell plasma membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The transmembrane protein (TM) acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The mature envelope protein (Env) consists of a trimer of SU-TM heterodimers attached by non-covalent interactions or by a labile interchain disulfide bond.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Transmembrane protein</molecule>
        <subcellularLocation>
            <location evidence="1">Virion membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Surface protein</molecule>
        <subcellularLocation>
            <location>Virion membrane</location>
            <topology>Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">The surface protein is not anchored to the viral envelope, but associates with the extravirion surface through its binding to TM. Both proteins are thought to be concentrated at the site of budding and incorporated into the virions possibly by contacts between the cytoplasmic tail of Env and the N-terminus of Gag (By similarity).</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Specific enzymatic cleavages in vivo yield mature proteins. Envelope glycoproteins are synthesized as an inactive precursor that is N-glycosylated and processed likely by host cell furin or by a furin-like protease in the Golgi to yield the mature SU and TM proteins. The cleavage site between SU and TM requires the minimal sequence [KR]-X-[KR]-R.</text>
    </comment>
    <dbReference type="EMBL" id="K00556">
        <property type="protein sequence ID" value="AAA46533.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF228552">
        <property type="protein sequence ID" value="AAF31475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S26388">
        <property type="entry name" value="S26388"/>
    </dbReference>
    <dbReference type="IntAct" id="Q85646">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006540">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019031">
        <property type="term" value="C:viral envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019064">
        <property type="term" value="P:fusion of virus membrane with host plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046718">
        <property type="term" value="P:viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019062">
        <property type="term" value="P:virion attachment to host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09909">
        <property type="entry name" value="HIV-1-like_HR1-HR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000328">
        <property type="entry name" value="GP41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00517">
        <property type="entry name" value="GP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-1169">Fusion of virus membrane with host cell membrane</keyword>
    <keyword id="KW-1168">Fusion of virus membrane with host membrane</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000239592" description="Envelope glycoprotein gp70">
        <location>
            <begin position="99"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000040779" description="Surface protein">
        <location>
            <begin position="99"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000040780" evidence="5">
        <location>
            <begin position="455"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000040781" description="Transmembrane protein">
        <location>
            <begin position="457"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="99"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="625"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="646"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fusion peptide" evidence="1">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Immunosuppression" evidence="1">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="426"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="511"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="site" description="Involved in binding to the cell receptor" evidence="6">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by host">
        <location>
            <begin position="454"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="557"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Complete loss of viral infectivity." evidence="3">
        <original>F</original>
        <variation>A</variation>
        <variation>S</variation>
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slight descrease in viral infectivity." evidence="3">
        <original>F</original>
        <variation>Y</variation>
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on viral infectivity." evidence="3">
        <original>G</original>
        <variation>E</variation>
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31475." evidence="6" ref="2">
        <original>N</original>
        <variation>K</variation>
        <location>
            <position position="3"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31475." evidence="6" ref="2">
        <original>T</original>
        <variation>I</variation>
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31475." evidence="6" ref="2">
        <original>D</original>
        <variation>S</variation>
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31475." evidence="6" ref="2">
        <original>A</original>
        <variation>P</variation>
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31475." evidence="6" ref="2">
        <original>S</original>
        <variation>T</variation>
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31475." evidence="6" ref="2">
        <original>E</original>
        <variation>G</variation>
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31475." evidence="6" ref="2">
        <original>E</original>
        <variation>D</variation>
        <location>
            <position position="363"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31475." evidence="6" ref="2">
        <original>YV</original>
        <variation>HA</variation>
        <location>
            <begin position="375"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31475." evidence="6" ref="2">
        <original>D</original>
        <variation>E</variation>
        <location>
            <position position="526"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF31475." evidence="6" ref="2">
        <original>G</original>
        <variation>S</variation>
        <location>
            <position position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12970432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="6281457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="6310154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="688" mass="77271" checksum="E76B3D38E2A01C0A" modified="1996-11-01" version="1" precursor="true">MPNHQSGSPTGSSDLLLDGKKQRAHLALRRKRRREMRKINRKVRRMNLAPIKEKTAWQHLQALIFEAEEVLKTSQTPQTSLTLFLALLSVLGPPPVSGESYWAYLPKPPILHPVGWGNTDPIRVLTNQTIYLGGSPDFHGFRNMSGNVHFEEKSDTLPICFSFSFSTPTGCFQVDKQVFLSDTPTVDNNKPGGKGDKRRMWELWLTTLGNSGANTKLVPIKKKLPPKYPHCQIAFKKDAFWEGDESAPPRWLPCAFPDQGVSFSPKGALGLLWDFSLPSPSVDQSDQIKSKKDLFGNYTPPVNKEVHRWYEAGWVEPTWFWENSPKDPNDRDFTALVPHTELFRLVAASRYLILKRPGFQEHEMIPTSACVTYPYVILLGLPQLIDIEKRGSTFHISCSSCRLTNCLDSSAYDYAAIIVKRPPYVLLPVDIGDEPWFDDSAIQTFRYATDLIRAKRFVAAIILGISALIAIITSFAVATTALVKEMQTATFVNNLHRNVTLALSEQRIIDLKLEARLNALEEVVLDLGQDVANLKTRMSTRCHANYDFICVTPLPYNASESWERTKAHLLGIWNDNEISYNIQELTNLIGDMSKQHIDTVDLSGLAQSFANGVKALNPLDWTQYFIFIGVGALLLVIVLMIFPIVFQCLAKSLDQVQSDLNVLLLKKKKGGNAAPAAEMVELPRVSYT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q9IZT2</accession>
    <name>PRO_MMTVC</name>
    <protein>
        <recommendedName>
            <fullName>Gag-Pro polyprotein</fullName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Matrix protein p10</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Phosphorylated protein pp21</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein p3</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein p8</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein n</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Capsid protein p27</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Nucleocapsid protein-dUTPase</fullName>
                <shortName>NC-dUTPase</shortName>
                <ecNumber evidence="9">3.6.1.23</ecNumber>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protease</fullName>
                <ecNumber evidence="5">3.4.23.-</ecNumber>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">gag-pro</name>
    </gene>
    <organism>
        <name type="scientific">Mouse mammary tumor virus (strain C3H)</name>
        <name type="common">MMTV</name>
        <dbReference type="NCBI Taxonomy" id="11759"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Pararnavirae</taxon>
            <taxon>Artverviricota</taxon>
            <taxon>Revtraviricetes</taxon>
            <taxon>Ortervirales</taxon>
            <taxon>Retroviridae</taxon>
            <taxon>Orthoretrovirinae</taxon>
            <taxon>Betaretrovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Mus musculus</name>
        <name type="common">Mouse</name>
        <dbReference type="NCBI Taxonomy" id="10090"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2000" name="J. Virol." volume="74" first="8876" last="8883">
            <title>Genetics of mouse mammary tumor virus-induced mammary tumors: linkage of tumor induction to the gag gene.</title>
            <authorList>
                <person name="Hook L.M."/>
                <person name="Agafonova Y."/>
                <person name="Ross S.R."/>
                <person name="Turner S.J."/>
                <person name="Golovkina T.V."/>
            </authorList>
            <dbReference type="PubMed" id="10982330"/>
            <dbReference type="DOI" id="10.1128/JVI.74.19.8876-8883.2000"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1987" name="Proc. Natl. Acad. Sci. U.S.A." volume="84" first="4298" last="4302">
            <title>Two efficient ribosomal frameshifting events are required for synthesis of mouse mammary tumor virus gag-related polyproteins.</title>
            <authorList>
                <person name="Jacks T."/>
                <person name="Townsley K."/>
                <person name="Varmus H.E."/>
                <person name="Majors J."/>
            </authorList>
            <dbReference type="PubMed" id="3035577"/>
            <dbReference type="DOI" id="10.1073/pnas.84.12.4298"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 359-591</scope>
        <scope>RIBOSOMAL FRAMESHIFT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1989" name="J. Virol." volume="63" first="2543" last="2549">
            <title>Analysis of gag proteins from mouse mammary tumor virus.</title>
            <authorList>
                <person name="Hizi A."/>
                <person name="Henderson L.E."/>
                <person name="Copeland T.D."/>
                <person name="Sowder R.C."/>
                <person name="Krutzsch H.C."/>
                <person name="Oroszlan S."/>
            </authorList>
            <dbReference type="PubMed" id="2542570"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-58; 65-116; 194-218; 227-241; 251-252; 270-276 AND 362-591</scope>
        <scope>PROTEOLYTIC CLEAVAGE (GAG-PRO POLYPROTEIN)</scope>
        <scope>MYRISTOYLATION AT GLY-2</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1994" name="Virology" volume="204" first="420" last="424">
            <title>The protein p30, encoded at the gag-pro junction of mouse mammary tumor virus, is a dUTPase fused with a nucleocapsid protein.</title>
            <authorList>
                <person name="Bergman A.C."/>
                <person name="Bjornberg O."/>
                <person name="Nord J."/>
                <person name="Nyman P.O."/>
                <person name="Rosengren A.M."/>
            </authorList>
            <dbReference type="PubMed" id="8091672"/>
            <dbReference type="DOI" id="10.1006/viro.1994.1547"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 497-512 AND 744-745</scope>
        <scope>RIBOSOMAL FRAMESHIFT</scope>
        <scope>SUBUNIT (NUCLEOCAPSID PROTEIN-DUTPASE)</scope>
        <scope>FUNCTION (NUCLEOCAPSID PROTEIN-DUTPASE)</scope>
        <scope>COFACTOR (NUCLEOCAPSID PROTEIN-DUTPASE)</scope>
    </reference>
    <comment type="function">
        <molecule>Matrix protein p10</molecule>
        <text evidence="10">Matrix protein.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Nucleocapsid protein p14: Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.</text>
    </comment>
    <comment type="function">
        <molecule>Capsid protein p27</molecule>
        <text evidence="10">Capsid protein.</text>
    </comment>
    <comment type="function">
        <molecule>Nucleocapsid protein-dUTPase</molecule>
        <text evidence="11">NC-dUTPase has dUTPase activity, thereby preventing incorporation of uracil into DNA.</text>
    </comment>
    <comment type="function">
        <molecule>Protease</molecule>
        <text evidence="5">The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dUTP + H2O = diphosphate + dUMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Magnesium ions are required for NC-dUTPase activity.</text>
    </comment>
    <comment type="activity regulation">
        <molecule>Protease</molecule>
        <text evidence="2">Inhibited by pepstatin A.</text>
    </comment>
    <comment type="subunit">
        <molecule>Matrix protein p10</molecule>
        <text evidence="10">Homodimer; when myristoylated.</text>
    </comment>
    <comment type="subunit">
        <molecule>Protease</molecule>
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subunit">
        <molecule>Nucleocapsid protein-dUTPase</molecule>
        <text evidence="9">NC-dUTPase is a homotrimer (PubMed:8091672).</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Matrix protein p10</molecule>
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Capsid protein p27</molecule>
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Nucleocapsid protein-dUTPase</molecule>
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="ribosomal frameshifting"/>
        <isoform>
            <id>Q9IZT2-1</id>
            <name>Gag-Pro polyprotein</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>P11283-1</id>
            <name>Gag-Pro-Pol polyprotein</name>
            <sequence type="external"/>
        </isoform>
        <isoform>
            <id>P11284-1</id>
            <name>Gag polyprotein</name>
            <sequence type="external"/>
        </isoform>
    </comment>
    <comment type="domain">
        <molecule>Gag-Pro polyprotein</molecule>
        <text evidence="10">Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Gag-p27 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101.</text>
    </comment>
    <comment type="PTM">
        <molecule>Protease</molecule>
        <text evidence="2">Released by autocatalytic processing.</text>
    </comment>
    <comment type="PTM">
        <molecule>Gag-Pro polyprotein</molecule>
        <text evidence="7">Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.</text>
    </comment>
    <comment type="PTM">
        <molecule>Gag-Pro polyprotein</molecule>
        <text evidence="7">Specific enzymatic cleavages in vivo yield mature proteins.</text>
    </comment>
    <comment type="miscellaneous">
        <molecule>Isoform Gag-Pro polyprotein</molecule>
        <text evidence="8 9">Produced by -1 ribosomal frameshifting between gag-pro.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="9"/>
    <dbReference type="EC" id="3.4.23.-" evidence="5"/>
    <dbReference type="EMBL" id="AF228552">
        <property type="protein sequence ID" value="AAF31473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M16766">
        <property type="protein sequence ID" value="AAA66623.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="BMRB" id="Q9IZT2"/>
    <dbReference type="SMR" id="Q9IZT2"/>
    <dbReference type="MEROPS" id="A02.010"/>
    <dbReference type="iPTMnet" id="Q9IZT2"/>
    <dbReference type="Proteomes" id="UP000006540">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019013">
        <property type="term" value="C:viral nucleocapsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039660">
        <property type="term" value="F:structural constituent of virion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016032">
        <property type="term" value="P:viral process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05482">
        <property type="entry name" value="HIV_retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.375.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003322">
        <property type="entry name" value="B_retro_matrix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038124">
        <property type="entry name" value="B_retro_matrix_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045345">
        <property type="entry name" value="Gag_p24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000721">
        <property type="entry name" value="Gag_p24_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034170">
        <property type="entry name" value="Retropepsin-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018061">
        <property type="entry name" value="Retropepsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008916">
        <property type="entry name" value="Retrov_capsid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008919">
        <property type="entry name" value="Retrov_capsid_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010999">
        <property type="entry name" value="Retrovr_matrix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02337">
        <property type="entry name" value="Gag_p10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00607">
        <property type="entry name" value="Gag_p24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19317">
        <property type="entry name" value="Gag_p24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00077">
        <property type="entry name" value="RVP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47836">
        <property type="entry name" value="SSF47836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47943">
        <property type="entry name" value="SSF47943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="SSF50630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="SSF57756"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0064">Aspartyl protease</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0519">Myristate</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0688">Ribosomal frameshifting</keyword>
    <keyword id="KW-0468">Viral matrix protein</keyword>
    <keyword id="KW-0543">Viral nucleoprotein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="initiator methionine" description="Removed; by host" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403622" description="Gag-Pro polyprotein">
        <location>
            <begin position="2"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403623" description="Matrix protein p10">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403624" description="Phosphorylated protein pp21">
        <location>
            <begin position="100"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403625" description="Protein p3">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403626" description="Protein p8">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403627" description="Protein n">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403628" description="Capsid protein p27">
        <location>
            <begin position="270"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403629" description="Nucleocapsid protein-dUTPase">
        <location>
            <begin position="497"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000403630" description="Protease">
        <location>
            <begin position="746"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A2" evidence="5">
        <location>
            <begin position="766"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CCHC-type 1" evidence="4">
        <location>
            <begin position="525"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CCHC-type 2" evidence="4">
        <location>
            <begin position="552"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="572"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PTAP/PSAP motif" evidence="10">
        <location>
            <begin position="305"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="581"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="active site" description="Protease; shared with dimeric partner" evidence="5">
        <location>
            <position position="771"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="7">
        <location>
            <begin position="99"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="7">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="7">
        <location>
            <begin position="228"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="7">
        <location>
            <begin position="254"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="7">
        <location>
            <begin position="269"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="7 9">
        <location>
            <begin position="496"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by viral protease" evidence="9">
        <location>
            <begin position="745"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="N-myristoyl glycine; by host" evidence="7">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="sequence variant">
        <original>E</original>
        <variation>K</variation>
        <location>
            <position position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P10258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P10271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="2542570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="3035577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="9">
        <source>
            <dbReference type="PubMed" id="8091672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="10"/>
    <evidence type="ECO:0000305" key="11">
        <source>
            <dbReference type="PubMed" id="8091672"/>
        </source>
    </evidence>
    <sequence length="860" mass="95593" checksum="EC4015AAA30F0797" modified="2000-10-01" version="1">MGVSGSKGQKLFVSVLQRLLSERGLHVKESSAIEFYQFLIKVSPWFPEEGGLNLQDWKRVGREMKKYAAEHGTDSIPKQAYPIWLQLREILTEQSDLVLLSAEAKSVTEEELEEGLTGLLSASSQEKTYGTRGTAYAEIDTEVDKLSEHIYDEPYEEKEKADKNEEKDHVRKVKKIVQRKENSEHKRKEKDQKAFLATDWNNDDLSPEDWDDLEEQAAHYHDDDELILPVKRKVDKKKPLALRRKPLPPVGFAGAMAEAREKGDLTFTFPVVFMGESDDDDTPVWEPLPLKTLKELQSAVRTMGPSAPYTLQVVDMVASQWLTPSDWHQTARATLSPGDYVLWRTEYEEKSKETVQKTAGKRKGKVSLDMLLGTGQFLSPSSQIKLSKDVLKDVTTNAVLAWRAIPPPGVKKTVLAGLKQGNEESYETFISRLEEAVYRMMPRGEGSDILIKQLAWENANSLCQDLIRPMRKTGTMQDYIRACLDASPAVVQGMAYAAAMRGQKYSTFVKQTYGGGKGGQGSEGPVCFSCGKTGHIKRDCKEEKGSKRAPPGLCPRCKKGYHWKSECKSKFDKDGNPLPPLETNAENSKNLVKGQSPSPTQKGDKGKDSGLNPEAPPFTIHDLPRGTPGSAGLDLSSQKDLILSLEDGVSLVPTLVKGTLPEGTTGLIIGRSSNYKKGLEVLPGVIDSDFQGEIKVMVKAAKNAVIIHKGERIAQLLLLPYLKLPNPIIKEERGSEGFGSTSHVHWVQEISDSRPMLHISLNGRRFLGLLDTGADKTCIAGRDWPANWPIHQTESSLQGLGMACGVARSSQPLRWQHEDKSGIIHPFVIPTLPFTLWGRDIMKEIKVRLMTDSPDDSQDL</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>